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 VOLUME Vol.05 Issue07 2025 

PAGE NO. 41-44 

DOI 10.37547/ajahi/Volume05Issue07-08 

 
 
 
 

Effect of The Zerox Immunostimulant On Cotton Yields 
 

Atoeva Rukhsora 

Bukhara State University, Muhammad Iqbol 11, 200118, Uzbekistan 

 

Atoeva Dilsora 

Bukhara State University, Muhammad Iqbol 11, 200118, Uzbekistan 

 

 

Received: 31 May 2025; Accepted: 27 June 2025; Published: 29 July 2025 

 

Abstract: In order to obtain high-quality yields from agricultural crops, various immunostimulants and biologically 
active substances are applied to the seeds before planting, as well as during different phenophases of the plants. 

In this article, three different norms (1-2-3 l/t) of the Zerox immunostimulant were applied to the seeds of the 
Bukhara-10 cotton variety before sowing and at different phenophases of the plant, and the effect on yield was 
evaluated. As a result, the most positive indicator was observed in variant 10, with an average three-year yield of 
46.0 q/ha. It was found that this yield was 7.6 q/ha higher than that of the control variant with the same number 
of seedlings.   

 

Keywords: Zerox immunostimulant, different number of seedlings, Bukhara-10 cotton variety, yield. 

 

Introduction: In order to obtain high and quality yields 
from crops in global agriculture, the demand for 
stimulants with various effects is increasing year by 
year. Nowadays, various stimulants and 
immunostimulants are used on 50-80% of the areas, 
where crops important for agriculture are grown [1].  

Growth regulators, as universal agents, play an 
important role not only in controlling plant growth and 
development but also in accelerating physiological and 
biochemical processes in plants. They enhance 
resistance to drought and help achieve higher crop 
yields under adverse conditions such as salinity [2]. 

Immunostimulants terminate the latent period in 
plants, accelerate hypocotyl germination in seeds, and 
increase the germination rate [3]. They accelerate stem 
growth and branching in plants during the budding, 
juvenil, virginal, and immature stages of the 
pregenerative period, as well as during the young 
generativ, mature generativ, and old generative stages 
of the generative period [4-5].  

Additionally, they enhance leaf area, dry mass, and net 
photosynthetic productivity [6-7]. Furthermore, 
immunostimulants improve valuable signs [8] and 
production in cotton [9]. One of these 

immunostimulants, Zerox, contains 3000 mg/l of 
colloidal silver and is used to enhance the immune 
system in plants [10].  

As a result of climate change and the negative impact 
of anthropogenic factors, plants are being infected with 
various phytoparasites. The most common bacterial 
disease in cotton is cotton gummosis. For example, R.O. 
Atoeva and others studied the role of 
immunostimulants in increasing the resistance of 
cotton to gummosis [11]. Furthermore, R.O. Atoeva 
investigated Zerox Immunostimulant increases 
relatively the resistance of cotton to root rot [12]. 

METHODS 

In order to determine the optimal rate of the 
immunostimulant Zeroks that positively affects the 
yield of cotton, different rates of the Zeroks 
immunostimulant (1.0-2.0-3.0 l/t; 1.0-2.0-3.0 l/ha) 
were applied to three different seedling of the 
Bukhara-10 cotton variety (80,000-90,000; 100,000-
110,000; and 120,000-130,000 plants per hectare). 
Also, the immunostimulant Fitovak was used at a rate 
of 200 ml/t and ml/ha as the standard. The control 
variant seeds was applied in drinking water, and during 
the growing season, it was suspended with water. 

 

https://doi.org/10.37547/ajahi/Volume05Issue07-08
https://doi.org/10.37547/ajahi/Volume05Issue07-08
https://doi.org/10.37547/ajahi/Volume05Issue07-08
https://doi.org/10.37547/ajahi/Volume05Issue07-08


American Journal Of Agriculture And Horticulture Innovations 42 https://theusajournals.com/index.php/ajahi 

American Journal Of Agriculture And Horticulture Innovations (ISSN: 2771-2559) 
 

 

Аll phеnological observаtions аnd analysis cаrried out 
in the invеstigation in laborаtory and fiеld condition 
were conducted on the bаsis of “Methods experiments 
of field” which written Uzbekistаn Cotton Science 
Research Institute [13] аnd Plаnt Insect Protection 
Center, аs well as, methods given in severаl other 
literature. Research dаtа wаs mаthemаtically 
processed in the method of B. Dospexov [14]. 

In scientific research, the experimental system 
consisted of 15 variants, 3 repetitions, each variant - 

270 m2 area , the total area of calculation was 4050 m2. 

In the experiment, hairy seedlings of the Bukhara-10 
cotton variety were applied with the Zerox 
immunostimulant at different norms (1-2-3 l/t of 
seedlings before sowing) and at different times (2-4 
true leaf, shading and flowering phases) together with 
1-2-3 l/ha + 0.15 l of PAV. The experimental system is 
presented in Table 1. 

 

 

Table 1. 

Experience system 

 

№ 

Variants 

and names 

of 

preparation

s 

A number 

of 

different 

seedlings 

thousand 

bushes/ha 

Application norms and terms, l/t; l/ha 

 

Applicati

on to 

seeds 

before 

planting 

  

 

2-4 true 

leaves  

 

 

Shading 

 

Flowerin

g 

 

1 

Control 

80-90 the seeds 

swell in 

water 

The above ground parts of the 

cotton plant were sprayed with 

water 

2 100-110 

3 120-130 

4 
Fitovak 

 

80-90 
200 

ml/t 

200 

ml/ha 

200  

ml/ha 

200 

ml/ha 
5 100-110 

6 120-130 

7 Zerox 

 

80-90 

1 l/t 

1 

l/ha+PАV  

0,15 l/ha 

1 

l/ha+PАV 

0,15 л/га 

1 l/ha 

+PАV 

0,15  

8 100-110 

9 120-130 

10 Zerox 80-90 

2 l/t 

2 

l/ha+PАV  

0,15 l/ha 

2 l/ha 

+PАV  

0,15 l/ha 

2 l/ha 

+PАV  

0,15 l/ha 

11 100-110 

12 120-130 

13 Zerox 80-90 3 l/t 3 

l/ha+PАV  

0,15 l/ha 

3 l/ha + 

PАV  0,15 

l/ha 

 

3 л/га 

+PАV  

0,15 l/ha 
14 100-110 

15 120-130 

 

RESULTS AND DISCUSSION 

Three different seedling thicknesses were left in the 
Bukhara-10 cotton variety in moderately saline soil 
conditions and Zeroks was applied to it at different 
norms and periods, and the effect on cotton yield was 
studied. 

It was found that with increasing seedling, the number 
of reproductive organs in shoot decreased. In the 
experiment, there were variants with seedling plants of 
80-90; 100-110 and 120-130 thousand plants/ha and 
the norms of Zerox 1-2-3 l/t; 1-2-3 l/ha + PAV 0,15 l/ha.  

Among all the variants, the highest number of shoots 
formation and development was observed in variants 

4-7-10-13 where the seedling number was left at 80-90 
thousand plants/ha. It was found that there were 3,0-
2,6-11,4-4,1 units more than control 1 variant, 
respectively. 

 It was found that the number of matured in variants 5-
8-11-14, with a seedling number of 100-110 thousand 
plants/ha, was 0,9-0,4-4,7-5,5 more bushes compared 
to the second variant.  

Also, in variants 6-9-12-15, with a seedling number of 
120-130 thousand plants/ha, the number of plants per 
hectare was 2,2-1,2-7,3-8,5 more plants compared to 
the third variant. When analyzing data on yield 
indicators for 2021-2022, control, Fitovak and Zeroks 
were used in the Bukhara-10 cotton variety with a 



American Journal Of Agriculture And Horticulture Innovations 43 https://theusajournals.com/index.php/ajahi 

American Journal Of Agriculture And Horticulture Innovations (ISSN: 2771-2559) 
 

 

seedling thickness of 80-90 thousand plants/ha.   

Table -2 

The effect of using Zeroks at different standard periods on the yield of cotton grown at different 

seedling thicknesses (2021-2023) 

  

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

As a result, the average three year productivity 
indicators in options 1-4-7-10-13 are: 38,1-41,4-39,8-
46,0-45,4 q/ha; in options 2-5-8-11-14 with a seedling 
number of 100-110 thousand plant/ha proportionally: 
36,0-39,6-38,8-43,6-43,0 q/ha; in variants 3-6-9-12-15, 
where the a seedling number was 120-130 thousand 
plants/ha, it was 35,1-38,3-37,4-42,2-42,0 q/ha, 
respectively. 

According to the data of 2023, when the Bukhara-10 
cotton variety was maintained at different seedling 
thicknesses and Zeroks was applied at different rates 
and periods (1-2-3 l/t; 1-2-3 l/ha+PAV 0,15 l/ha), it was 
observed that there was a significant difference in the 
cotton yield between the options. 

In the last phase of the cotton vegetation, the cotton 
harvest leaves a seedling number of 80-90 thousand 
plant/ha, control, standard, Zeroks 1-2-3 l/t; 1-2-3 
l/ha+PAV 0,15 l/ha used in the 1-4-7-10-13 variants,  

 

 

 

respectively: 40,8-46,3-44,1-52,5-50,5 q/ha; in variants 
2-5-8-11-14, where the seedling number is 100-110 
thousand plant/ha, respectively: 36,5-44,2-42,5-50,8-
49,5 q/ha; The seedling number was 35,9-40,4-40,1-
49,7-47,6 q/ha in the 3-6-9-12-15 variants, where 120-
130 thousand plants/ha were left. 

CONCLUSION 

According to the conducted three year studies, the 
highest cotton yield was observed in the variant with a 
seedling number of 80-90 thousand plants/ha and the 
optimal norm of application of Zerox (2 l/t: 2 l/ha + PAV 
0,15 l/ha) (Table 2). 

 The increase in seedling number and the use of the 
immunnostimulant Zerox below or above the standard 
level during all growth and development periods of the 
Bukhara-10 cotton variety led to a significant decrease 
in biological and economic cotton yields. As a result, the 
most positive indicator was observed in variant 10, with 
an average three-year yield of 46.0 q/ha. It was found 
that this yield was 7.6 q/ha higher than that of the 

№ Variants 

 

A 

number 

of 

different 

seedlings 

thousan

d 

plants/h

a 

2021 2022 2023 An 

average 

of three 

years, 

total 

harvest 

q/ha 

Produc 

tivity 

differenc

e, 

q/ha, ± 

total 

harves

t, 

q/ha 

total 

harves

t, 

q/ha 

total 

harves

t, 

q/ha 

1 Control 

(water) 

80-90     38,8 34,6 40,8 38,1 0,0 

2 100-110 37,5 33,9   36,5 36,0 0,0 

3 120-130 35,7 33,7 35,9 35,1 0,0 

4 Fitovak 

 200 ml/t; 

ml/hа 

80-90 40,5 37,5 46,3 41,4 +3,3 

5 100-110 39,7 34,9 44,2 39,6 +3,6 

6 120-130 39,0 35,6 40,4 38,3 +3,2 

7 Zerox 1 

l/t, l/hа 

80-90 40,0 35,4 44,1 39,8 +1,7 

8 100-110 39,0 35,0 42,5 38,8 +2,8 

9 120-130 38,5 33,5 40,1 37,4 +2,3 

10 Zerox 2 l/t, 

l/hа 

80-90 45,5 40,0 52,5 46,0 +7,9 

11 100-110 42,0 38,0 50,8 43,6 +7,6 

12 120-130 40,5 36,5 49,7 42,2 +7,1 

13 Zerox 3 l/t, 

l/hа 

80-90 45,2 40,4 50,5 45,4 +7,3 

14 100-110 42,3 37,3 49,5 43,0 +7,0 

15 120-130 42,0 36,5 47,6 42,0 +6,9 

 НСР05  1,69 

s/hа 

1,21 

s/hа 

1,26 

ц/hа 

1,28 ц/hа  

 P%  4,2% 3,4% 2,8% 3,2 %  



American Journal Of Agriculture And Horticulture Innovations 44 https://theusajournals.com/index.php/ajahi 

American Journal Of Agriculture And Horticulture Innovations (ISSN: 2771-2559) 
 

 

control variant with the same number of seedlings. 

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