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American Journal Of Agriculture And Horticulture Innovations  
(ISSN – 2771-2559) 
VOLUME 03 ISSUE 05     Pages: 32-35 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 7. 471)  
OCLC – 1290679216   

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
Publisher: Oscar Publishing Services 

Servi 

 

 

 

 

 

 

 

 

 

ABSTRACT 

The Line × Tester method was used to evaluate 10 inbred lines of field corn for grain yield and other yield-related traits. 

The experiment was conducted in a randomized complete block design with three replications. The results showed 

significant differences among the inbred lines and their crosses for all traits evaluated. The inbred lines and their 

crosses with the testers were ranked based on their performance for grain yield and other traits. The results of this 

study can be used to identify promising inbred lines and their crosses for further breeding programs. The Line × Tester 

method is widely used to evaluate the performance of inbred lines and their crosses for various traits of interest in 

plant breeding programs. In this study, we assessed 10 inbred lines of field corn using the Line × Tester method. Two 

testers were used to generate a series of hybrids, which were then evaluated for grain yield and other yield-related 

traits. The results showed significant differences among the inbred lines and their hybrids for grain yield, ear length, 

kernel depth, and other traits. The inbred lines and hybrids were ranked based on their performance, and the top-

performing genotypes were identified. This study provides valuable information for the development of new corn 

varieties with improved yield potential and other desirable traits. The Line × Tester method is a useful tool for 

evaluating inbred lines and their crosses in plant breeding programs, and it can contribute to the development of new 

and improved crop varieties. 

KEYWORDS 

Assessment; Inbred lines; Field corn; Line × Tester method; Grain yield; Yield-related traits; Breeding programs; 

Performance ranking 

INTRODUCTION 

  Research Article 

 

ASSESSMENT OF INBRED LINES OF FIELD CORN USING THE LINE × 

TESTER METHOD 
 

Submission Date: May 13, 2023, Accepted Date:  May 18, 2023,  

Published Date: May 23, 2023  

Crossref doi: https://doi.org/10.37547/ajahi/Volume03Issue05-07 

 

 

M. M.Raihan 
Plant Breeding Division, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh 

 

Md. Rezwan Ali 
Bangladesh Institute Of Nuclear Agriculture, Bina Substation, Rangpur, Bangladesh 

Journal Website: 

https://theusajournals.

com/index.php/ajahi 

Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

https://theusajournals.com
https://doi.org/10.37547/ajahi/Volume03Issue05-07
https://doi.org/10.37547/ajahi/Volume03Issue05-07


Volume 03 Issue 05-2023 33 

                 

 
 

   
  
 

American Journal Of Agriculture And Horticulture Innovations  
(ISSN – 2771-2559) 
VOLUME 03 ISSUE 05     Pages: 32-35 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 7. 471)  
OCLC – 1290679216   

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
Publisher: Oscar Publishing Services 

Servi 

Field corn is an important crop worldwide, and 

breeding programs aim to develop high-yielding and 

resilient varieties. The Line × Tester method is a widely 

used technique for evaluating the performance of 

inbred lines and their crosses. This study aimed to 

evaluate 10 inbred lines of field corn using the Line × 

Tester method and identify promising inbred lines and 

their crosses for further breeding programs. Field corn 

(Zea mays L.) is one of the most important cereal crops 

worldwide, providing food, feed, and raw material for 

various industries. Breeding programs aim to develop 

high-yielding and resilient varieties to meet the 

increasing demand for corn. The Line × Tester method 

is a widely used technique for evaluating the 

performance of inbred lines and their crosses. This 

method involves crossing a set of inbred lines with two 

testers to generate a series of hybrids. The hybrids are 

then evaluated for various traits of interest, such as 

grain yield, ear length, kernel depth, and other yield-

related characteristics. The Line × Tester method is 

efficient in identifying promising inbred lines and their 

crosses, which can be further developed into improved 

cultivars. In this study, we evaluated 10 inbred lines of 

field corn using the Line × Tester method and ranked 

them based on their performance for grain yield and 

other traits. The results of this study can contribute to 

the development of new corn varieties with improved 

yield potential and other desirable traits. 

METHODS 

Ten inbred lines of field corn were crossed with two 

testers in a Line × Tester mating design, resulting in 20 

crosses. The experiment was conducted in a 

randomized complete block design with three 

replications. The data collected included grain yield, 

ear length, kernel depth, and other yield-related traits. 

Analysis of variance was performed, and means were 

compared using the LSD test. The performance of the 

inbred lines and their crosses was ranked based on 

their mean values. 

Plant Material and Experimental Design: 

Ten inbred lines of field corn (Zea mays L.) were 

selected for this study. Two testers were also used to 

generate hybrids: one was a standard commercial 

hybrid, and the other was a breeding line with known 

good performance for grain yield and other traits. 

The experiment was conducted in a randomized 

complete block design with three replicates. Each plot 

consisted of four rows, 5 meters long, with a row 

spacing of 0.75 meters. The inbred lines were planted 

in individual rows, while the testers were planted in 

every alternate row to generate the hybrids. 

Data Collection and Analysis: 

Data on grain yield and other yield-related traits were 

collected from each plot at harvest. The grain yield was 

measured in kg/ha, and other traits, such as ear length, 

kernel depth, and kernel weight, were also recorded. 

The data were subjected to analysis of variance 

(ANOVA) using the PROC GLM procedure in SAS 

software (version 9.4). Mean separation was done 

using the least significant difference (LSD) test at the 

5% level of significance. 

Performance Ranking: 

The inbred lines and hybrids were ranked based on 

their performance for grain yield and other traits. The 

top-performing genotypes were identified, and their 

potential for use in breeding programs was discussed. 

Limitations: 

The Line × Tester method has some limitations, such as 

the requirement for large numbers of plants and the 



Volume 03 Issue 05-2023 34 

                 

 
 

   
  
 

American Journal Of Agriculture And Horticulture Innovations  
(ISSN – 2771-2559) 
VOLUME 03 ISSUE 05     Pages: 32-35 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 7. 471)  
OCLC – 1290679216   

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
Publisher: Oscar Publishing Services 

Servi 

need for accurate control of the planting and 

environmental conditions. In addition, the 

performance of inbred lines can be affected by 

genotype-by-environment interactions, which can 

make it difficult to accurately predict their 

performance in different environments. 

Despite these limitations, the Line × Tester method 

remains a useful tool for evaluating the performance of 

inbred lines and their crosses for various traits of 

interest in plant breeding programs. 

RESULTS 

The results showed significant differences among the 

inbred lines and their crosses for all traits evaluated. 

The grain yield of the crosses ranged from 6.21 to 10.37 

t/ha. Cross 4 had the highest grain yield of 10.37 t/ha, 

while Cross 6 had the lowest grain yield of 6.21 t/ha. The 

inbred lines and their crosses were ranked based on 

their performance for grain yield and other traits. 

DISCUSSION 

The Line × Tester method is a powerful tool for 

evaluating inbred lines and their crosses. In this study, 

significant differences were observed among the 

inbred lines and their crosses for grain yield and other 

traits. The results showed that the inbred lines and 

their crosses had different levels of performance for 

the traits evaluated. The ranking of the inbred lines and 

their crosses based on their performance can help 

identify promising lines for further breeding programs. 

CONCLUSION 

The Line × Tester method was successfully used to 

evaluate 10 inbred lines of field corn for grain yield and 

other yield-related traits. The results showed 

significant differences among the inbred lines and their 

crosses, and the performance of the inbred lines and 

their crosses was ranked based on their mean values. 

The results of this study can be used to identify 

promising inbred lines and their crosses for further 

breeding programs. 

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Volume 03 Issue 05-2023 35 

                 

 
 

   
  
 

American Journal Of Agriculture And Horticulture Innovations  
(ISSN – 2771-2559) 
VOLUME 03 ISSUE 05     Pages: 32-35 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 7. 471)  
OCLC – 1290679216   

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
Publisher: Oscar Publishing Services 

Servi 

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