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American Journal Of Agriculture And Horticulture Innovations  
(ISSN – 2771-2559) 
VOLUME 03 ISSUE 03     Pages: 11-14 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 7. 471)  
OCLC – 1290679216   

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
Publisher: Oscar Publishing Services 

Servi 

 

 

 

 

 

 

 

 

 

 

ABSTRACT 

Importance of in vitro microclonal propagation of ornamental, fruit and medicinal plants growing in our republic. 

KEYWORDS 

Micro clonal reproduction, ginkgo biloba, chakanda, flavonoids, carotene, sterols, vitamins. 

 

INTRODUCTION 

At present, great attention is being paid to in vitro 

reproduction of medicinal plants, cell culture, and 

microclonal reproduction. Research in this direction is 

being conducted in many laboratories of the 

developed countries of the world. Also, the 

development of pharmaceutical production in 

Uzbekistan, the development of the technology of 

drugs based on local raw materials, and the 

implementation of these works are currently 

considered one of the important tasks of 

representatives of this field. Currently, microclonal 

propagation of plants in vitro and short-term 

propagation of isolated tissue cells without a genetic 

variety of viruses are aimed at obtaining seedlings with 

a high coefficient. This technology is used to obtain 

millions and more somatic shoots and continuously 

grow seedlings in the laboratory year-round. In the last 

years of the last century, great success was achieved in 

this field, in the production of biologically active 

substances. Another advantage of the extraction of 

  Research Article 

 

PROSPECTS OF MICROCLONAL PROPAGATION OF MEDICINAL PLANT 

SPECIES 
 

Submission Date: March 07, 2023, Accepted Date:  March 12, 2023,  

Published Date: March 17, 2023  

Crossref doi: https://doi.org/10.37547/ajahi/Volume03Issue03-03 

 

 

D. T. Yakhshiboeva 
Doctor Of Philosophy (Phd) Teacher Of The Department Of "Biology” Navoi State Pedagogical Institute, 

Uzbekistan 

 

N.D.Jumaniyozova 
Student Of The Department Of "Biology” Navoi State Pedagogical Institute, Uzbekistan 

Journal Website: 

https://theusajournals.

com/index.php/ajahi 

Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

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https://doi.org/10.37547/ajahi/Volume03Issue03-03


Volume 03 Issue 03-2023 12 

                 

 
 

   
  
 

American Journal Of Agriculture And Horticulture Innovations  
(ISSN – 2771-2559) 
VOLUME 03 ISSUE 03     Pages: 11-14 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 7. 471)  
OCLC – 1290679216   

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
Publisher: Oscar Publishing Services 

Servi 

secondary metabolites by this method is that, under 

certain conditions, where the plant does not have the 

opportunity to grow itself (in cold or hot climate 

regions), its cells can be grown throughout the year. 

In recent years, consistent reforms have been 

implemented in our republic regarding the protection 

of medicinal plants, rational use of natural resources, 

establishment of medicinal plant plantations and their 

processing. 750 species of more than 4.3 thousand 

plants belonging to the local flora are considered 

medicinal, 112 of them are registered for use in 

scientific medicine, 70 of them are actively used in the 

pharmaceutical industry. In 2019, 48 million USD worth 

of processed medicinal plant products were exported. 

At the same time, the analyzes show the need to 

protect medicinal plants, establish their plantations, 

and create an additional value chain through 

processing. Based on this, many decisions of the 

President of the Republic of Uzbekistan regarding 

medicinal plants have been adopted. In particular, the 

decision of April 10, 2020 "On protection of wild 

medicinal plants, cultivation, processing and rational 

use of available resources"; Decree of the President of 

the Republic of Uzbekistan "On measures to expand 

the scope of scientific research on cultivation and 

processing of medicinal plants, development of their 

seed production" 26, 2020 November decision PQ-

4901; The forestry of Uzbekistan is distinguished by the 

abundance and variety of flora and fauna, including 

unique ornamental and fruit trees and medicinal 

plants. Decree of the President of the Republic of 

Uzbekistan "On the establishment of the State 

Committee of Forestry of the Republic of Uzbekistan" 

and "Organization of the State Committee of Forestry 

of the Republic of Uzbekistan" on" was announced. 

Implementation of this decree and decision: Effective 

and rational use of forest resources, their restoration, 

introduction of large-scale reproduction, protection of 

forests and their effective use, ornamental and fruit 

trees and shrubs on an industrial scale provides 

expansion of cultivation of plantations. Creation of 

modern new methods of reproduction of seedlings of 

ornamental trees and shrubs adapted to the climatic 

conditions of our republic, development of 

scientifically based promising technologies, creation of 

exportable plants, increases the efficiency of selection 

work. The area and the number of species of many 

decorative, fruit and medicinal plants growing in the 

territory of our republic is decreasing due to the misuse 

and neglect of people. In order to prevent this, many 

decisions are taken by our government. 

But due to the small number of some promising plants 

and the difficulty of their natural reproduction, there 

are problems in the creation and reproduction of 

plantations. In order to overcome these problems, 

professors and masters of Fergana State University are 

conducting scientific research in the field of 

microclonal reproduction of several useful plants. In 

particular, the biological properties of ginkgo biloba 

and chakanda (Hippophae rhamnoides L.) from 

ornamental and fruit plants are being studied and 

preliminary work on their microclonal propagation in 

vitro is being carried out. Chakanda (Hippophae 

rhamnoides L.) - a shrub called chakanda or chirkana, 

belonging to the family of cycads. It reaches 3-5 meters 

in height. It is dioecious, thorny, sertican, and its trunk 

and branches are covered with hairs. The leaves are 

located in a row, the band is short, the length is 6-8 cm. 

The fruit is round, orange or lanceolate, one-seeded, 3-

5 mm in diameter, juicy. It blooms and leaves in May-

April. The flowering of one bush lasts 10-12 days. A 

special tissue is located at the base of the calyx and 

serves to extract nectar. Bees and other insects eat the 

nectar and pollen of small flowers. Like all parts of the 

plant, honey collected from its flowers is a medicinal 

and delicious food. The fruit ripens in September-

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Volume 03 Issue 03-2023 13 

                 

 
 

   
  
 

American Journal Of Agriculture And Horticulture Innovations  
(ISSN – 2771-2559) 
VOLUME 03 ISSUE 03     Pages: 11-14 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 7. 471)  
OCLC – 1290679216   

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
Publisher: Oscar Publishing Services 

Servi 

October and remains on the plant throughout the 

winter. Chakanda fruit is included in the "Red Book" 

and is considered a nutritious source of food for rare 

birds. does. The largest and thickest groves are 

scattered in the oasis of the Zarafshan River, in the 

Zarafshan State Reserve, which was established in 

1975. 

In the Fergana valley, there are small groves in a limited 

area, and isolated bushes of small bushes are found 

above the Kampirrovot reservoir (in the Karadarya 

basin) and along some tributaries of Shakhimardonsoy. 

Its area is shrinking as a result of anthropogenic 

influence. The oil obtained from the fruit of Chakanda 

plant is mainly used in pharmaceuticals. The fruit 

contains vitamin C, E, B1, B2, carotene, fatty acids, folic 

acid and other organic acids. Chakanda fruit is very 

juicy, the kernel contains 16%, the fruit 9%, and the seed 

12% fat. The oil is dark, orange in color, with a unique 

smell and taste. Chakanda oil contains 40-100 mg% 

carotene, 180-250 mg% carotenoids. It contains high 

levels of vitamin E. This vitamin participates in the 

body's metabolism. Chakanda fruit and seed oil is used 

as pain reliever, skin tissue regeneration, wound 

healing and scurvy treatment. They are mainly used in 

the treatment of various skin and gynecological 

diseases. It has also been found that the leaves of 

Chakanda contain additives, vitamin C and other active 

substances. Herbal sources are also used for sore 

throat, toothache and inflammation of the oral cavity. 

According to ethnobotanical data, chakanda plant is 

also used in the treatment of gastritis, hepatitis, and 

bronchitis. It is necessary to use the natural reserves of 

this plant sparingly, to protect its limited areas and to 

engage in measures to increase it. Since chakanda is a 

dioecious plant, it is difficult to identify male and 

female before planting. Often, when planted in fields, 

there are many male plants, and as a result, 

productivity decreases. In order to prevent this, target 

plants can be planted with microclonal propagation 

and high yield can be achieved. Ginkgo biloba is the 

oldest of all ancient plants on the planet. It belongs to 

the ginkgo family of the gymnosperms division and is 

the last surviving representative of this family. For 

more than 300 million years, Ginko has been fighting 

for survival, and now the plant is in danger of 

extinction. Ginkgo biloba is a dioecious tree, up to 40 

m tall and up to 1 m in diameter. The seed looks like a 

round, grainy fruit. 

Fruits and seeds are eaten. The leaves are heart-

shaped, green, and turn yellow in autumn. Because of 

its beautiful appearance, it is often planted as an 

ornamental tree. It grows wild in some parts of South 

China. However, its leaf and wood remains were found 

in the Upper Permian and Triassic deposits of Central 

Asia. So, Ginkgo biloba grew in our region at that time, 

but later it disappeared. Currently, Ginkgo is cultivated 

as an ornamental plant in our country. However, due to 

the low fertility (40-50%) and unfavorable 

environmental conditions, reproduction is somewhat 

difficult. The unique feature of this plant is related to 

many elements included in its chemical composition. 

The chemical composition of plant leaves includes 

various flavonoids, biflavonoids, nonacosan, sterols, 

various amino acids, essential oils, a complex of 

vitamins, a complex of macro and microelements. The 

results of use and research show that preparations 

made on the basis of Ginkobiloba have a strong 

therapeutic effect on body systems. There are studies 

on the effectiveness of plant extract in inhibiting the 

growth of cancer cells. In addition, Ginkgo is used to 

improve brain activity, strengthen memory, 

depression, cognitive development, treat Alzheimer's 

disease, eye diseases, reduce pain, regulate blood flow, 

heart and nerve diseases, fight cancer, and in 

astrology. Since Ginkgo biloba does not reproduce 

from its vegetative organs, and its seeds have low 

https://doi.org/10.37547/ajahi/Volume03Issue03-03
https://scholar.google.co.in/scholar?q=PROSPECTS%20OF%20MICROCLONAL%20PROPAGATION%20OF%20MEDICINAL%20PLANT%20SPECIES
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Volume 03 Issue 03-2023 14 

                 

 
 

   
  
 

American Journal Of Agriculture And Horticulture Innovations  
(ISSN – 2771-2559) 
VOLUME 03 ISSUE 03     Pages: 11-14 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 7. 471)  
OCLC – 1290679216   

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 
Publisher: Oscar Publishing Services 

Servi 

germination, microclonal reproduction of this plant is 

very effective. 

It is known that ginkgo biloba and chakanda plants 

grow slowly, their seed germination is low, and 

damage by pests and high temperature is common. 

Microclonal reproduction of these plants allows to 

shorten the growth phase and obtain disease-resistant 

virus-free plants. Due to the great importance of the 

above-mentioned plants in pharmaceuticals, in the 

future, if microclonal reproduction of them is started, 

it will be possible to prepare and export high-quality 

sources of raw materials for the pharmaceutical 

industry. 

CONCLUSION 

In conclusion, microclonal propagation of plants from 

leaves, stems, buds and other vegetative organs in 

vitro is of great economic importance. 

REFERENCES 

1. R. Artikova, S. Murodova. Agricultural 

biotechnology. Tashkent-2010 

2. O'. Pratov, Q. Jumayev. Systematics of higher 

plants, 2003 

3. S. Meliboyev, M. Israilov, Z. Isomiddinov, H. 

Abdunazarov, SH. Tursunov. "Medicinal plants 

found in the flora of Uzbekistan". 

4. G`. Khomidov, M. Makhmudov, R. Maksudova. 

"Medicinal plants of the Fergana Valley, their 

effective use and protection". Fergana 2014 

5. H. Kholmatov "Medicinal plants of Uzbekistan". T. 

"Medicine", 1976. 

 

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