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 VOLUME Vol.05 Issue06 2025 

PAGE NO. 8-10 

DOI 10.37547/ajbspi/Volume05Issue06-02 

 
 
 
 

Technology and Qualitative Analysis of Liquid Extract 

Obtained on The Basis of Plant Raw Materials 

Polygonum Hydropiper L. And Hypericum Perforatum L. 
 

Yuldasheva Shakhlo Khabibullayevna 

Tashkent Pharmaceutical Institute, Uzbekistan 

 

 

Received: 14 April 2025; Accepted: 10 May 2025; Published: 12 June 2025 

 

Abstract: This research work is devoted to the study of the technology and qualitative analysis of obtaining liquid 
extract from Polygonum hydropiper and Hypéricum perforátum plant raw materials. Percolation was used in 
liquid extract extraction. Ethyl alcohol of different concentrations was used as an extract. When conducting quality 
analysis, a spectrophotometric method was used. 

 

Keywords: Liquid extract, flavonoids, Polygonum hydropiper and Hypéricum perforátum, percolation, 
spectrophotometry. 

 

Introduction: Drug preparations with a twisting effect, 
obtained from plant raw materials, are now widely 
used. Agents with a twisting effect are among the drugs 
that have a local anti-inflammatory effect. They are 
used in inflammatory processes of the skin and mucous 
membranes [1]. Therefore, when we carried out our 
research work, we selected Herba Polygoni 
hydropiperis and Hypéricum perforátum from plant 
raw materials with a twisting effect by chemical 
composition. The Polygonum hydropiper has been 
used since ancient times as a medicinal plant, with 
which the Greeks and Romans, al-Khimik, used it. 
Polygonum hydropiper contains glycosides, vitamins C, 
K, carotene, flavanoids, sugars, organic acids, 
astringent bitter compounds and other substances. In 
folk medicine, tincture is used in malaria, diarrhea, 
hemorrhoids and as a bleeding suppressor and 
painkiller and as a medicine for various wounds [5]. The 
Hypéricum perforátum product contains 10-12.8 
percent additives,0.1-0.4 percent anthracene unums, 
flavonoids (hyperoside, rutin, quercitrin, isocversitrin, 
quercetin, myricetin, etc.), 0.1 - 03 percent essential oil, 
55 mg percent carotene, 1151.8 mg percent vitamin C, 
~4 mg percent choline, very small amounts of alkaloids, 
and up to 10 percent tar. Hypéricum perforátum 
products, on the other hand, have anti-inflammatory, 
spasmolytic analgesic, antiseptic and twisting effects. 

In medicine, it is used in the treatment of 
gastrointestinal (colitis, diarrhea), oral diseases 
(gingivitis and stomatitis) and burns of II and III degrees, 
as well as for rinsing the mouth. The plant surface of 
the plant has a bactericidal effect [1,4]. 

The purpose of the work. Obtaining liquid extract from 
Polygonum hydropiper and Hypéricum perforátum 
plant raw materials and conducting its qualitative 
analysis is the goal of our research work.  

METHODS 

When obtaining a liquid extract, on the 
recommendation of pharmacologists from the 
following selected plant raw materials, herba Polygoni 
hydropiperis and herba Hypérici perforátum in the 
same proportion were pulled out and ground at a level 
of 3-5 mm spruce. Two different capacities of ethyl 
alcohol were selected as an extract (40% and 70%). The 
percolation method was used in obtaining liquid 
extract [2,3]. Liquid extracts were studied on the basis 
of methods given in their appearance, dry residue, 
alcohol concentration, density, heavy metal content 
O’zR DF, XI DF [4,5]. Quantitative analysis of the 
resulting liquid extracts was analyzed in a 
spectrophotometric way by the average amount of 
flavonoids (in the calculation of the rutin standard 
substance) [2,4].  

 

https://doi.org/10.37547/ajbspi/Volume05Issue06-02
https://doi.org/10.37547/ajbspi/Volume05Issue06-02
https://doi.org/10.37547/ajbspi/Volume05Issue06-02
https://doi.org/10.37547/ajbspi/Volume05Issue06-02


American Journal of Applied Science and Technology 9 https://theusajournals.com/index.php/ajast 

American Journal of Applied Science and Technology (ISSN: 2771-2745) 
 

 

As standard, rutin flavanoid is used, in which the 
maximum absorption corresponds to the maximum 
absorption of the sample being examined with the 
aluminum chloride complex. Measurements of optical 
density were carried out on the UB-spectrophotometer 
"Agilent Technology-8253" (Germany), at a wavelength 
of 350-450 nm, in cuvettes 10 mm thick.  

Preparation of the probing solution. 1ml of the probing 
liquid extract was placed in a 25ml volume measuring 
flask, 5ml of 96% ethyl alcohol, 5ml of 5% aluminium 
chloride was released from a 70% solution of ethyl 
alcohol. After 10 minutes, a solution of 2 ml of 5% acetic 
acid in 70% ethyl alcohol was added. The volume of the 
solution was reached and mixed with 70% ethyl alcohol 
to the line of the measuring flask. The optical density of 
the solution obtained after 30 minutes was measured 
in a 10 mm thick cuvette at a wavelength of 408nm on 
a spectrophotometer. A sample of a compensatory 
solution was prepared-this is a solution of 1 ml of the 
liquid extract, 5 ml of 96% ethyl alcohol and 2 ml of 5% 

acetic acid in 70% ethyl alcohol, put in a 25 ml flask and 
brought to the line of the measuring flask with 70% 
ethyl alcohol. 

Working standard sample solution preparation (WSS). 
Approximately 25 mg (exact drawer) of WSS rutin is 
obtained, dried to a constant weight at 135°C, placed in 
a 100 ml volume flask, dissolved in heated 80 ml of 96% 
ethyl alcohol, then cooled and transported to the line 
of the measuring flask with that 96% ethyl alcohol and 
mixed.  

At the same time, after 30 minutes, the optical density 
of 1 ml of a comparable solution (isn rutin) is measured 
using a sample of a compensatory solution (1 ml of 
liquid extract, 5 ml of 96% ethyl alcohol and 2 ml of 5% 
acetic acid in 70% ethyl alcohol, put in a 25 ml flask and 
brought to the line of the measuring solution flask with 
70% ethyl alcohol).   

The sum of flavanoids contained in the liquid extract is 
found through the following formula:  

10000125100100

251

0

0

0

0




=




=

A

PmA

A

PmA
X

in this:  

А — is the optical density of a probing solution; 

А0 — is the optical density of a comparable solution; 

m0 — is the mass of the routine standard sample, mg; 

Р — The amount of rutin on the WSS, indicated in the 
quality certificate, is %. 

RESULTS AND DISCUSSION 

Qualitative analysis of the obtained liquid extracts was 
studied on the basis of the methods given in O’zR DF, XI 

DF. In this case, a liquid extract obtained using 40% 
ethyl alcohol looked like this in appearance: a clear 
liquid of dark green, brown color; a dry residue of 
2.76%, an alcohol concentration of 35%, a density of 
0.94 g/ml, the amount of heavy metals did not exceed 
0.001%. The liquid extract obtained using 70% ethyl 
alcohol looked the following in appearance: a clear 
liquid of dark green, brown color; a dry residue of 
2.22%, an alcohol concentration of 67.5%, a density of 
0.92 g/ml, the amount of heavy metals did not exceed 
0.001%. The results are shown in Table 1. 

Liquid extract quality indicators 

Table 1 

№ 

 

Appearance Alcohol 

concentrate 

Amount of dry 

residue 

Density Total amount 

sum of 

flavonoids 

Liquid extract 

(40% ethyl 

alcohol) 

clear liquid of 

dark green, 

brown color 

35% 

 

2,76 

 

0,94 

 

2,3085% 

 

Liquid extract 

(70% ethyl 

alcohol) 

clear liquid of 

dark green, 

brown color 

67,5% 

 

2,22 

 

0,92 

 

2,5796% 

 

Quantitative analysis of the resulting liquid extracts 
was analyzed by spectrophotometry method in terms 
of the average amount of flavonoids (in the standard 
rutin calculation). Based on the results of a quantitative 
analysis, the average amount of flavonoids in liquid 
extract obtained using 40% ethyl alcohol (in standard 
rutin accounting) was 2.3085%; the average amount of 

flavonoids in liquid extract obtained using 70% ethyl 
alcohol (in standard rutin accounting) was 2.5796%. 

CONCLUSION  

Liquid extracts from Polygonum hydropiper and 
Hypéricum perforátum plant raw materials with the 
participation of an extragent of different 
concentrations were obtained by percolation and 



American Journal of Applied Science and Technology 10 https://theusajournals.com/index.php/ajast 

American Journal of Applied Science and Technology (ISSN: 2771-2745) 
 

 

qualitative analysis of it was carried out. According to 
the results, a liquid extract obtained using 70% ethyl 
alcohol was found to be optimal. 

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