







































Volume 04 Issue 05-2024 56 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 05 PAGES: 56-62 

SJIF IMPACT FACTOR (2022: 5. 705) (2023: 6.534) (2024: 7.7) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

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ABSTRACT 

Even the oldest examples of writing, created in the years before Christ, contain information about the healing 

properties of plants. Some natural drugs considered valuable were isolated in pure form decades ago. But there are 

many plants that have not yet been studied and whose medicinal properties have not been scientifically tested. 

KEYWORDS 

Phlogogens, medicinal substance, alkaloids, histamine, dextran, leaf sap. 

INTRODUCTION

Around the world, a number of scientific research 

works are being carried out in order to improve the 

effectiveness of treatment and the prevention of side 

effects of anti-inflammatory drugs. Despite the wide 

range of anti-inflammatory drugs currently used in 

practice, there is still a high demand for new drugs with 

high pharmacological activity, low toxicity and minimal 

side effects.  [1,11- 73, 36]. 

The purpose of the study.  Study of the effect of a 

hydrogel containing a field ivy extract on the exudative 

stage of inflammation. 

 

 One of such plants is field ivy belonging to the 

ivy family, widespread in all regions of Uzbekistan. The 

people of Central Asia have different names for this 

plant. Turkmens are charapechak, Kazakhs are 

  Research Article 

 

ANTI-EXUDATIVE EFFECTS OF HYDROGEL PRESERVING FIELD OVEN 

EXTRACT 
 

Submission Date: May 21, 2024, Accepted Date:  May 26, 2024,  

Published Date: May 31, 2024  

Crossref doi: https://doi.org/10.37547/ajbspi/Volume04Issue05-09 

 

 

Yakubova Umida Bakhtiyarovna  
Urganch Branch of Tashkent Medical Academy, Uzbekistan 

Journal Website: 

https://theusajournals.

com/index.php/ajbspi 

Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

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Volume 04 Issue 05-2024 57 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 05 PAGES: 56-62 

SJIF IMPACT FACTOR (2022: 5. 705) (2023: 6.534) (2024: 7.7) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

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shermatik, Turks are chermashik, and Russians are 

vyunok polevoy. The Institute of Chemistry of Plant 

Substances of the Academy of Sciences of Uzbekistan 

is of great service in finding medicinal plants and 

extracting alkaloids from them. The effect of medicinal 

plants on the body depends on the amount of chemical 

compounds in its composition. These compounds 

accumulate in different amounts in different parts of 

the plant. Medicinal substances are collected in the 

shoots, leaves or fruits of some plants, in the roots or 

bark of some plants. Therefore, the part of the plants 

with the most biologically active substances is 

harvested  [9,10- 37, 372].  

 The field ivy plant is a perennial weed growing 

in the ground or creeping, the length of the stem 

reaches 40-100 cm. The leaves are arcuate, spear-like 

with a sharp tip. Field ivy blooms in March-April. 

Blooms in March-August. The flower is white or pink, 

trumpet-shaped. The fruit ripens in June-September. 

The appearance of the fruit is wide ovoid, hairless 

length is 6-8 mm. Field ivy grows abundantly on 

irrigated land. It is spread everywhere in our republic. 

In order to prepare medicines from the plant, the 

above-ground part of the plant is collected and dried in 

a cool, shady place. The above-ground parts of the 

plant contain flavonoids, caffeic acid, carotene, vitamin 

C, resins and some alkaloids. Many medicinal 

preparations are prepared from this plant. Undried leaf 

juice of the plant mixed with beef fat was used as a 

cure for lung and ear diseases.  Bruised branches are 

washed with a decoction made from the above-ground 

part of the plant. The decoction was also used as an 

anti-scabie medicine. A powder made from the leaves 

is sprinkled on wounds and sores  [4,5-32, 284].        

 The scientific significance of the results of the 

research is that it is used for the first time in the form 

of a gel from local plant sources, the possibility of 

finding among them highly effective agents that 

stimulate anti-inflammatory properties, and the main 

aspects of the mechanism of action of the gel 

containing the extract of field ivy are highlighted [13, 

88]. 

METHOD 

As the object of the study, a gel prepared from a 

complex of biologically active substances isolated from 

the above-ground parts of the field ivy plant was taken. 

The anti-inflammatory effect of ivy extract 

preservative gel was compared to ibuprofen gel. Due 

to the complexity of the inflammatory process, it is 

difficult to choose drugs that will act against it  [6, 340]. 

 The anti-inflammatory effect of the studied 

drugs is determined by the difference between the 

growth of the paws of experimental and comparison 

group animals or the difference between the 

amputated paws of mice. White rats with a mass of 155-

180 g were used to perform the tasks set before us. 

Formalin, dextran, histamine, serotonin and 

carrageenan were used as phlogogenic agents.  It is 

known that physiologically active substances - 

inflammatory mediators (histamine, serotonin, 

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Volume 04 Issue 05-2024 58 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 05 PAGES: 56-62 

SJIF IMPACT FACTOR (2022: 5. 705) (2023: 6.534) (2024: 7.7) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

bradykinin) are released under the influence of various 

agents that injure tissues. These active substances play 

an important role in the process of inflammation. 

 It can be seen that the inflammatory process 

can be induced by introducing the above-mentioned 

agents into the body. In the initial period of 

inflammation, a significant exudation phase is 

observed. After the primary alteration, in response to 

the impact of the injurious agent, the increase in 

vascular permeability, the transfer of blood plasma and 

shaped elements from the site of inflammation to the 

surrounding tissues, causes edema, which is 

characteristic of inflammation  [2, 221-222]. 

 Histamine and serotonin are of practical 

importance for 15-20 minutes after exposure to 

inflammatory agents, and then the release of 

proteolytic enzymes in injured cells, the increase in the 

concentration of biogenic amines leads to an increase 

in the activity of the kallikrein-kinin system. Kinins are 

neurovasoactive polypeptide substances that have 

different effects on smooth muscles, kidney, lung and 

heart activity, permeability and tone of blood vessels 

wall. 

 Among the phlogogenic agents named above, 

formalin is widely used by many researchers, because 

the inflammation caused by formalin occurs quickly, 

the maximum swelling is observed after 5-6 hours and 

is quickly reabsorbed. In addition, inflammation 

induced by formalin is very similar to the inflammatory 

process that occurs in the human body  [8, 12-646, 

654]. 

 0.1 ml of 0.1% histamine and 0.2% serotonin 

solution was injected under the aponeurosis of the 

hind paw of the rat using a syringe. Paw size of rats was 

measured oncometrically before and every 60 minutes 

for 4 hours after administration of phlogogenic agents, 

and for the last time after 24 hours. Inflammation 

under the influence of histamine and serotonin 

appears very quickly, the maximum swelling of the 

paws is observed after 1-1.5 hours and is reabsorbed in 

the last 24 hours. 

RESULTS  

In experiments, we conducted studies to determine 

the antiexudative effect of field ivy extract gel in 

comparison with ibuprofen gel in aseptic models of 

histamine and serotonin. The results of these series of 

experiments show that subplantar administration of 

histamine caused a 72.3% increase in rat paw size 

compared to the initial size. 

 The anti-inflammatory activity of the ivy extract 

was 38.3%, and the anti-inflammatory activity of the 

ibuprofen gel was 28.8%, 1 hour after the start of the 

gel application in histamine-induced animals. It can be 

seen that the used gels significantly affected the 

exudation process caused by histamine. (table 1) 

Histamine (produced in basophilic and mast cells) 

exerts its effect through 2 different types of N1 and N2 

receptors. Pain occurs when histamine acts on N1 

receptors. When acting on N2 receptors, it increases 

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Volume 04 Issue 05-2024 59 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 05 PAGES: 56-62 

SJIF IMPACT FACTOR (2022: 5. 705) (2023: 6.534) (2024: 7.7) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

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the production of prostaglandins and thromboxane, 

reduces chemotaxis and phagocytic activity of 

neutrophils, and reduces the release of lysosomal 

enzymes by neutrophils. Acting through both types of 

receptors, histamine dilates precapillary arterioles at 

the site of inflammation, narrows vessels in the lungs, 

and increases the permeability of the vascular wall in 

the skin and some organs. 

Table 1 

Anti-inflammatory effects of 5% ivy extract hydrogel and 5% ibuprofen gels against 

histamine-induced inflammation 

 

Groups 
Initial paw 

size is cm3 

 

After 1 

hour, the 

size of the 

paw is cm3 

 

After 2 

hours, the 

size of the 

paw is cm3 

 

After 3 

hours, the 

size of the 

paw is cm3 

 

After 4 

hours, the 

size of the 

paw is cm3 

 

Control 

 

100% 72,3% 57,4% 38,6% 19,8% 

FI 100% 38,3% 48,3% 58,9% 65,0% 

IBP 

 

100% 28,8% 34,5% 38,5% 40,0% 

 

Thus, the gel containing the extract of field ivy had a 

significant anti-inflammatory effect induced by 

histamine. 

 The anti-inflammatory activity of the tested 

drug in the serotonin model was highly effective, and 

its effect was close to the anti-inflammatory effect 

induced by histamine. (table 2). The anti-inflammatory 

activity of the ivy extract gel is superior to ibuprofen 

gel. The results of the calculation of the anti-

inflammatory activity of these drugs showed that. 

 Serotonin (produced in fat cells of the skin and 

other tissues and delta cells of platelets) exerts its 

effects through serotoninergic receptors. These 

effects include pain, narrowing of venules, increased 

permeability of the vessel wall, and thrombus 

formation  [3, 45-46]. 

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Volume 04 Issue 05-2024 60 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 05 PAGES: 56-62 

SJIF IMPACT FACTOR (2022: 5. 705) (2023: 6.534) (2024: 7.7) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

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Table 2 

 

Serotonin-induced anti-inflammatory effects of  5% ivy extract hydrogel and 5% 

ibuprofen gel 

 

Groups 

 

Initial 

paw size 

is cm3 

 

After 30 

min, the 

size is 

cm3 

 

After 60 

min, the 

size is 

cm3 

 

After 90 

min, the 

size is 

cm3 

 

After 

120 

min, the 

size is 

cm3 

 

After 

180 

min, the 

size is 

cm3 

 

After 

240 

min, the 

size is 

cm3 

 

Control 

 

100% 74,1% 90,6% 78,8% 54,1% 38,8% 23,5% 

FI 100% 39,7% 41,5% 47,8% 47,8% 69,7% 90% 

IBP 

 

100% 28,6% 33,7% 35,8% 35,8% 63,6% 80% 

 

It is known from the literature that inflammation 

caused by dextran develops very quickly (within 1-1.5 

hours) and returns quickly. In our experiments, rats 

were injected with 0.1 ml of a 6% dextran solution under 

the aponeurosis of the hind paw. The size of the paws 

was measured by oncometric method before injection 

of dextran solution and every 60 minutes after 

injection, the last time after 24 hours (table 3). 

Table 3 

Effects of ivy extract and ibuprofen-containing gels on dextran-induced inflammation 

 

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Volume 04 Issue 05-2024 61 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 05 PAGES: 56-62 

SJIF IMPACT FACTOR (2022: 5. 705) (2023: 6.534) (2024: 7.7) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

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Groups Paw size, cm3 

Дастлабки 1 соат 2 соат 3 соат 4 соат 

Control 
0,96±0,04 1,85±0,08* 1,90±0,09* 1,77±0,08* 1,66±0,06* 

FI (1%) 0,90±0,04 1,70±0,09* 1,75±0,08* 1,67±0,08* 1,58±0,08* 

FI (3%) 0,91±0,03 1,52±0,07* 1,53±0,08* 1,43±0,07* 1,35±0,09* 

FI (5%) 0,95±0,05 1,49±0,07* 1,51±0,05* 1,41±0,04* 1,30±0,05* 

Ibuprofen  
0,95±0,05 1,53±0,08* 1,55±0,09* 1,45±0,10* 1,37±0,10* 

 

CONCLUSION 

1. Field ivy extract preservative gel showed high 

antiexudative activity in aseptic inflammations induced 

by various phlogogens when applied to the surface. At 

the same time, it is not inferior to ibuprofen, a well-

known non-steroidal anti-inflammatory agent, in terms 

of its pharmacological activity. 

2. The absence of significant changes in hematological 

and biochemical parameters during continuous use of 

the gel made from the extract of field ivy showed that 

its side effects are few. 

3. Field ivy extract preservation gel has practical value 

as a potential anti-inflammatory drug. 

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Якубова У.Б. Противовоспалительная 

активность экстракта вьюнка полевого при 

местном применении. // Назарий ва клиник 

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Volume 04 Issue 05-2024 62 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 05 PAGES: 56-62 

SJIF IMPACT FACTOR (2022: 5. 705) (2023: 6.534) (2024: 7.7) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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СВОЙСТВА И ПРИМЕНЕНИЕ В МЕДИЦИНЕ. 

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