







































Volume 04 Issue 05-2024 19 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 05 PAGES: 19-21 

SJIF IMPACT FACTOR (2022: 5. 705) (2023: 6.534) (2024: 7.7) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

 

 

 

 

 

 

 

 

 

 

ABSTRACT 

Biotransformation enzyme genes play an important role in metabolism and processing of foreign substances 

(xenobiotics) in the body. Their role in tumor progression is their ability to modulate metabolic pathways by affecting 

the processing of xenobiotics, which may affect the development of tumors. This is a current topic of research in the 

field of oncology. 

KEYWORDS 

acute leukemia, chronic leukemia, molecular genetics, gene, polymorphism, heterozygosity, wild allele, minor allele, 

MDR1, rs1045642. 

INTRODUCTION

The MDR1 gene encodes a protein called P-

glycoprotein (P-gp), which is a member of the ATP-

binding cassette (ABC) transporter family. P-gp plays a 

critical role in cellular drug efflux, actively pumping 

various drugs and toxins out of cells, thereby 

promoting drug resistance in cancer treatment. The 

  Research Article 

 

ROLE OF THE GENE FOR XENOBIOTICS BIOTRANSFORMATION ENZYME 

IN TUMOR PROGRESSION IN ACUTE LEUKEMIA 
 

Submission Date: May 07, 2024, Accepted Date:  May 12, 2024,  

Published Date: May 17, 2024  

Crossref doi: https://doi.org/10.37547/ajbspi/Volume04Issue05-04 

 

 

Z.A.Sabitova 
Master's Degree Tashkent International University Kimyo, Uzbekistan 

 

P.A.Ganijonov 
Assistant At The Department Of Physiology, Fergana Medical Institute Of Public Health, Uzbekistan 

 

K. T. Boboev 
Doctor Of Medical Sciences., Professor Doctor Of Medical Sciences Professor Republican Specialized 

Hematology Scientific And Practical Hematology And Medical Center, Uzbekistan 

 

Journal Website: 

https://theusajournals.

com/index.php/ajbspi 

Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

https://doi.org/10.37547/ajbspi/Volume03Issue03-01
https://scholar.google.co.in/scholar?q=
https://www.mendeley.com/search/?page=1&query=
https://theusajournals.com/
https://doi.org/10.37547/ajbspi/Volume04Issue05-04
https://theusajournals.com/index.php/ajbspi
https://theusajournals.com/index.php/ajbspi


Volume 04 Issue 05-2024 20 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 05 PAGES: 19-21 

SJIF IMPACT FACTOR (2022: 5. 705) (2023: 6.534) (2024: 7.7) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

C3435T polymorphism in the MDR1 gene involves a 

substitution of cytosine (C) for thymine (T) at position 

3435 of the sequence and is associated with changes in 

P-gp expression and function, potentially affecting its 

ability to efficiently pump drugs out of cells. Variations 

in P-gp expression or function due to the C3435T 

polymorphism could potentially influence the 

metabolism of endogenous substrates or 

environmental toxins, which could influence cellular 

processes associated with leukemogenesis. Moreover, 

altered P-gp expression or function due to the C3435T 

polymorphism may disrupt cellular homeostasis, 

potentially leading to increased genomic instability or 

disruption of DNA repair mechanisms, which are 

known to contribute to the development of leukemia 

[1,2,3,4, 5]. 

PURPOSE OF THE STUDY 

To develop a PCR method to detect the C3435T 

polymorphism of the MDR1 gene and evaluate its role 

in the formation of acute and chronic leukemia. 

MATERIALS AND METHODS OF RESEARCH 

The study included 103 patients suffering from acute 

leukemia, who were registered at the Republican 

Specialized Scientific and Practical Medical Center for 

Hematology of the Ministry of Health of the Republic 

of Uzbekistan, and 90 conditionally healthy individuals 

were selected as a control group, of which 50 people 

did not have oncohematological diseases or history of 

thrombosis. 

RESULTS 

Carriers of the C allele showed a significant decrease in 

the likelihood of developing the disease by 41% (OR = 

0.59, 95% CI: 0.39-0.89; p = 0.01), while the minor T 

allele of the MDR1 C3435T gene significantly increased 

the risk 1.69 times (OR = 1.69; 95% CI: 1.13-2.55; p = 0.01). 

Analysis of the C3435T polymorphism showed that the 

TT genotype increases the risk of the disease by 2.31 

times (OR = 2.31; 95% CI: 1.09-4.89; p = 0.027), while the 

CC genotype demonstrates a 44% reduction in risk (OR 

= 0.56, 95% CI: 0.31-1.03, p = 0.06). It is noteworthy that 

in this study, the TT genotype was found to be a 

significant risk factor for the development of the 

disease. 

In our study, the main group of patients (n=103) was 

divided into subgroups depending on the acute (first 

subgroup) or chronic (second subgroup) course of 

leukemia. Regarding the pathogenetic significance of 

the C3435T polymorphism of the MDR1 gene in the first 

subgroup, carriers of the C allele had a noticeable 

reduction in the likelihood of developing the disease by 

48% (OR = 0.52, 95% CI: 0.32-0.84; p = 0.008). 

Conversely, the minor T allele of the MDR1 C3435T 

polymorphism significantly increased the risk of the 

disease by 1.93 times (OR = 1.93; 95% CI: 1.19-3.15; p = 

0.008), becoming a significant risk factor for the 

development of acute leukemia in our research. On the 

other hand, our study did not reveal a statistically 

significant relationship between the second group 

(chronic leukemia) and the C3435T polymorphism of 

the MDR1 gene (χ2<3.85; p>0.05). 

https://doi.org/10.37547/ajbspi/Volume03Issue03-01
https://scholar.google.co.in/scholar?q=
https://www.mendeley.com/search/?page=1&query=


Volume 04 Issue 05-2024 21 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 05 PAGES: 19-21 

SJIF IMPACT FACTOR (2022: 5. 705) (2023: 6.534) (2024: 7.7) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

CONCLUSION 

In our study, the minor allele (T) of the C3435T 

polymorphism of the MDR1 gene demonstrated a 

significant positive association with acute leukemia 

(OR=1.93; 95% CI: 1.19-3.15, χ2=7.13; p=0.008). On the 

contrary, no statistically significant relationship was 

observed between the second group (chronic 

leukemia) and the C3435T polymorphism of the MDR1 

gene (χ2<3.85; p>0.05). 

 

REFERENCES 

1. Коркина Ю. С., Валиев Т. Т., Варфоломеева С. Р. 

Клинические и молекулярно-биологические 

особенности острых лейкозов у детей до 1 года 

//Онкогематология. – 2022. – Т. 17. – №. 2. – С. 23-

29. 

2. Лесниченко И. Ф. и др. Клиническое значение 

уровня матриксной металло-протеиназы-9 

(ММП-9) в плазме аспиратов костного мозга 

больных острым миелоидным лейкозом 

//Вестник гематологии. – 2017. – Т. 13. – №. 3. – С. 

49-49. 

3. Мамаев Н. Н. и др. Молекулярный мониторинг 

течения острых миелоидных лейкозов по 

уровню экспрессии гена WT1 после аллогенной 

трансплантации гемопоэтических стволовых 

клеток //Клиническая онкогематология. 

Фундаментальные исследования и клиническая 

практика. – 2018. – Т. 8. – №. 3. – С. 309-320. 

4. Аббасова М. Т., Гаджиев А. М. Изменения 

показателей обмена железа в крови крыс при 

электромагнитном облучении в дециметровом 

диапазоне //Гематология и трансфузиология. – 

2019. – Т. 64. – №. 3. – С. 274-282. 

5. Аманкулова А. А., Макимбетов Э. К. Лечение 

острого лимфобластного лейкоза у детей на 

современном этапе (Обзор литературы) 

//Вестник Кыргызско-Российского Славянского 

университета. – 2020. – Т. 20. – №. 1. – С. 11-15. 

 

 

https://doi.org/10.37547/ajbspi/Volume03Issue03-01
https://scholar.google.co.in/scholar?q=
https://www.mendeley.com/search/?page=1&query=

