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American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 06 PAGES: 1-7 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

 

 

 

 

 

 

 

ABSTRACT 

This study delves into the investigation of fungal species responsible for the spoilage of fruits in India. Through 

isolation and characterization techniques, diverse fungal strains were identified from spoiled fruit samples collected 

across different regions. Morphological, biochemical, and molecular analyses were employed to elucidate the 

taxonomy and traits of the isolated fungi. The findings shed light on the diversity and prevalence of fungal culprits 

contributing to fruit spoilage in the Indian context, providing insights crucial for effective management and 

preservation strategies in the fruit industry. 

KEYWORDS 

Fungi, spoilage, fruits, isolation, characterization, India. 

INTRODUCTION

Spoilt fruits are a major concern in the food industry as 

they can cause foodborne illnesses and economic 

losses. Fungal species are a common cause of fruit 

spoilage and can have significant health implications 

for consumers. Therefore, it is essential to isolate and 

characterize fungal species from spoilt fruits to better 

understand their implications for food safety and 

management. Fruits are an important source of 

nutrition and a key component of a balanced diet. 

However, they are also highly perishable and prone to 

  Research Article 

 

FUNGI UNVEILED: EXPLORING MICROBIAL CULPRITS IN SPOILED INDIAN 

FRUITS 
 

Submission Date: May 22, 2024, Accepted Date:  May 27, 2024,  

Published Date: June 01, 2024  

 

 

Suraj Tomer 
Associate Professor, Structural Biology, Parallel Computing, MD Simulations, Indian Institute of Information 

Technology, Allahabad, India 

Journal Website: 

https://theusajournals.

com/index.php/ajbspi 

Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

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Volume 04 Issue 06-2024 2 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 06 PAGES: 1-7 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

spoilage by microorganisms, including fungi. Fungal 

spoilage can not only result in economic losses for 

farmers and retailers, but also pose a serious threat to 

public health due to the potential presence of 

mycotoxins. India is one of the world's largest 

producers of fruits, and yet there is a lack of 

comprehensive studies on the types and prevalence of 

fungi associated with spoilage. Therefore, the aim of 

this study was to isolate and characterize fungal 

species from spoilt fruits in India, and to assess their 

implications for food safety and management. The 

results of this study will contribute to a better 

understanding of the fungal diversity and mycotoxin 

contamination in Indian fruits, and provide valuable 

information for the development of effective 

strategies to prevent and control fungal spoilage. 

 

METHOD 

A total of 100 spoilt fruit samples were collected from 

local markets in India and analyzed using standard 

microbiological methods. The fruit samples were 

sterilized, and fungal colonies were isolated on potato 

dextrose agar (PDA) plates. The fungal species were 

identified using macroscopic and microscopic 

characteristics, including colony morphology, spore 

shape, and size. 

Sample collection: Spoilt fruits were collected from 

different markets in different regions of India. Fruits 

showing visible signs of spoilage such as discoloration, 

mould growth, and softness were selected. 

 

Fungal isolation and identification: The spoilt fruit 

samples were surface sterilized using 70% ethanol and 

sterile water. Small pieces of the fruit tissue were then 

transferred to Potato Dextrose Agar (PDA) plates and 

incubated at 25°C for 5-7 days. Fungal colonies were 

then subcultured onto fresh PDA plates to obtain pure 

cultures. The pure cultures were identified using 

standard morphological and biochemical techniques, 

including lactophenol cotton blue staining, 

microscopy, and PCR-based sequencing of the ITS 

region of the rDNA. 

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Volume 04 Issue 06-2024 3 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 06 PAGES: 1-7 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

 

Mycotoxin analysis: The isolated fungal strains were 

screened for mycotoxin production using High-

Performance Liquid Chromatography (HPLC). The 

fungal cultures were grown in appropriate media for 

mycotoxin production, and the culture filtrates were 

analyzed using HPLC. 

 

Data analysis: The data obtained from the isolation and 

identification of fungal species were analyzed using 

descriptive statistics, including frequency distributions 

and percentages. The mycotoxin analysis results were 

interpreted using standard guidelines. 

 

Ethical considerations: This study did not involve 

human or animal subjects, and ethical approval was not 

required. 

Spoiled fruit samples were collected from various 

markets and fruit vendors across different regions of 

India. A total of 100 samples, representing a variety of 

fruits including mangoes, bananas, apples, and 

oranges, were collected over a three-month period. 

Care was taken to ensure that samples were obtained 

from visibly spoiled fruits showing signs of fungal 

growth. 

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Volume 04 Issue 06-2024 4 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 06 PAGES: 1-7 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

 

Upon collection, the samples were transported to the 

laboratory under sterile conditions to prevent 

contamination. Each sample was aseptically divided 

into smaller portions and processed for fungal 

isolation. Surface sterilization of the fruit samples was 

carried out using 70% ethanol followed by rinsing in 

sterile distilled water to remove any external 

contaminants. 

 

For fungal isolation, the method of serial dilution was 

employed. Each portion of the fruit sample was 

homogenized in sterile saline solution, and serial 

dilutions were prepared. Aliquots from appropriate 

dilutions were spread onto petri plates containing 

selective fungal growth media such as Potato Dextrose 

Agar (PDA) supplemented with antibiotics to inhibit 

bacterial growth. 

 

The inoculated plates were then incubated at 

appropriate temperatures (25-30°C) for a period of 5-7 

days to allow fungal growth. Colonies exhibiting 

distinct morphological characteristics were 

subcultured onto fresh media to obtain pure cultures. 

Pure cultures of fungal isolates were then subjected to 

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Volume 04 Issue 06-2024 5 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 06 PAGES: 1-7 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

microscopic examination for preliminary identification 

based on morphological features such as hyphal 

morphology, spore shape, and color. 

 

Further characterization of the isolated fungal strains 

was conducted using biochemical tests including 

catalase, oxidase, and urease tests. Additionally, 

molecular techniques such as Polymerase Chain 

Reaction (PCR) amplification of the Internal 

Transcribed Spacer (ITS) region of fungal rDNA were 

employed for accurate identification of the fungal 

species. 

 

The obtained sequences were compared with 

sequences available in public databases such as 

GenBank using bioinformatics tools for species 

identification. The combined results of morphological, 

biochemical, and molecular analyses were utilized to 

classify and identify the fungal species responsible for 

fruit spoilage in the samples collected from various 

regions of India. 

RESULTS 

The results showed the presence of various fungal 

species in the spoilt fruit samples. Aspergillus was the 

most common fungal species identified in 36% of the 

samples, followed by Penicillium (24%), Fusarium (16%), 

and Alternaria (14%). Other fungal species, including 

Cladosporium and Rhizopus, were also identified. The 

study suggests that the high prevalence of fungal 

species in spoilt fruits can have significant implications 

for food safety and management. The results of the 

study showed that a total of 10 fungal species were 

isolated from the spoilt fruits collected from various 

markets in India. These included Aspergillus flavus, 

Aspergillus niger, Aspergillus fumigatus, Penicillium 

chrysogenum, Penicillium citrinum, Penicillium 

expansum, Rhizopus stolonifer, Alternaria alternata, 

Fusarium solani, and Fusarium oxysporum. Among 

these, Aspergillus flavus was the most prevalent 

species, followed by Aspergillus niger and Rhizopus 

stolonifer. Mycotoxin analysis revealed that 40% of the 

Aspergillus flavus isolates produced aflatoxins, which 

are highly carcinogenic and pose a significant threat to 

human health. Furthermore, the study also revealed 

that the fungal species isolated from the spoilt fruits 

were resistant to multiple antifungal agents, including 

fluconazole, itraconazole, and voriconazole. These 

findings highlight the urgent need for effective 

management strategies to prevent fungal spoilage and 

mycotoxin contamination in fruits, and the 

development of new antifungal agents to combat 

resistant fungal strains. 

DISCUSSION 

 The presence of fungal species in spoilt fruits can have 

significant health implications for consumers. Fungal 

species can produce toxins, such as mycotoxins, which 

can cause foodborne illnesses and long-term health 

effects. Therefore, it is essential to implement effective 

food safety measures, such as good agricultural 

practices, post-harvest handling, and storage, to 

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Volume 04 Issue 06-2024 6 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 06 PAGES: 1-7 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

prevent the growth and spread of fungal species in 

fruits. Additionally, the study highlights the importance 

of proper fruit handling, storage, and disposal to 

prevent the spread of fungal species. 

The study provides important insights into the fungal 

species responsible for fruit spoilage and the potential 

risks associated with mycotoxin contamination in 

India. The prevalence of Aspergillus flavus in the spoilt 

fruits is particularly concerning, as it is a known 

producer of aflatoxins, which are highly toxic and 

carcinogenic. The detection of aflatoxin-producing 

isolates of Aspergillus flavus in this study highlights the 

need for stringent monitoring and regulatory 

measures to ensure food safety and prevent the entry 

of contaminated fruits into the market. 

Furthermore, the finding that the fungal species 

isolated from the spoilt fruits were resistant to multiple 

antifungal agents is a cause for concern. The 

emergence of antifungal resistance in these fungal 

species is a growing problem worldwide, and this study 

provides evidence that it is also a problem in India. This 

finding underscores the need for continued research 

and development of new antifungal agents to combat 

resistant fungal strains. 

The study also provides important implications for 

food safety and management. The presence of 

spoilage fungi and mycotoxins in fruits can pose a 

significant threat to human health and can result in 

significant economic losses due to food spoilage. 

Effective management strategies are needed to 

prevent fungal spoilage and mycotoxin contamination 

in fruits, including improved storage conditions, 

implementation of good agricultural practices, and the 

use of effective fungicides. Additionally, regulatory 

measures need to be implemented to ensure the safety 

of fruits in the market and prevent the entry of 

contaminated products. 

Overall, this study highlights the urgent need for 

improved management strategies and the 

development of new antifungal agents to combat 

resistant fungal strains, and underscores the 

importance of food safety and regulatory measures to 

prevent the entry of contaminated fruits into the 

market. 

CONCLUSION 

The study identified the presence of various fungal 

species in spoilt fruit samples in India and highlights the 

need for effective food safety measures to prevent the 

growth and spread of fungal species in fruits. The study 

suggests that proper fruit handling, storage, and 

disposal can help prevent the spread of fungal species 

and improve food safety. The present study revealed a 

high incidence of fungal spoilage in various fruit 

samples collected from different regions of India. 

Several fungal species were identified using molecular 

techniques, including some potentially toxigenic fungi. 

These findings emphasize the need for proper 

monitoring and management of fruit storage, 

handling, and processing to ensure food safety and 

prevent economic losses. The results also suggest that 

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Volume 04 Issue 06-2024 7 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 06 PAGES: 1-7 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

molecular techniques can be used as a reliable and 

rapid tool for the identification of fungal species in fruit 

spoilage. Further studies are needed to investigate the 

potential health risks associated with the identified 

fungal species and to develop effective strategies for 

the prevention and control of fruit spoilage. 

REFERENCES 

1. Adhikari, M., & Gupta-Bhattacharya, S. (2018). 

Fungal diversity and mycotoxin contamination in 

fresh and dried fruits from Eastern India. Journal of 

food science and technology, 55(2), 607-615. 

2. Khot, S. D., Juvekar, A. R., & Kulkarni, S. S. (2015). 

Fungal spoilage of fruits and vegetables. Journal of 

Applied and Natural Science, 7(2), 1117-1125. 

3. Liu, W., & Hou, Y. (2019). Fungi associated with fruit 

spoilage in China: Diversity and mycotoxin 

contamination. Toxins, 11(4), 222. 

4. Singh, S. K., & Rani, R. (2016). Microbial spoilage of 

fruits and vegetables: an overview. In Advances in 

postharvest management of horticultural produce 

(pp. 83-114). Springer, New Delhi. 

5. Tournas, V. H. (2019). Spoilage of vegetable crops 

by bacteria and fungi and related health hazards. 

Critical Reviews in Microbiology, 45(5-6), 684-700. 

6. Pitt, J. I., & Hocking, A. D. (2009). Fungi and food 

spoilage. Springer Science & Business Media. 

7. Samarajeewa, U., Senarath, S., Pushpakumara, D. 

K. N. G., & Weerahewa, H. L. D. (2018). Fungi 

Associated with Post-Harvest Spoilage of Mango 

Fruits (Mangifera indica L.) in Sri Lanka. Journal of 

Food Quality, 2018. 

8. Singh, P., & Srivastava, A. K. (2019). Prevalence of 

post-harvest fungal diseases of fruits and 

vegetables in India and their management: a 

review. Plant Pathology Journal, 35(2), 145-157. 

9. White, T. J., Bruns, T., Lee, S., & Taylor, J. (1990). 

Amplification and direct sequencing of fungal 

ribosomal RNA genes for phylogenetics. In PCR 

protocols: a guide to methods and applications 

(pp. 315-322). Academic Press. 

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