







































Volume 04 Issue 01-2024 113 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 113-117 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

ABSTRACT 

The article presents the results of a study of biologically active substances in a dry extract obtained from the flower 

buds of the medicinal plant saffron Crocus sativus L. The GSX method was developed to determine the amount of 

biologically active substances in the dry extract. 

KEYWORDS 

Dry extract, saffron, chromatography, standard samples. 

INTRODUCTION

  Research Article 

 

QUANTITATIVE DETERMINATION OF BIOLOGICALLY ACTIVE 

COMPOUNDS FROM DRY EXTRACT OF SAFFRON (CROCUS SATIVUS L.) 

BY GAS CHROMATOGRAPHY 
 

Submission Date: January 21, 2024, Accepted Date:  January 26, 2024,  

Published Date: January 31, 2024  

Crossref doi: https://doi.org/10.37547/ajbspi/Volume04Issue01-17 

 

 

M.O.Shomaksudova 
Tashkent Pharmaceutical Institute, Uzkfiti, Institute Of Bioorganic Chemistry Of The Academy Of Sciences Of 

The Republic Of Uzbekistan 

 

A.A.Tulaganov 
Tashkent Pharmaceutical Institute, Uzkfiti, Institute Of Bioorganic Chemistry Of The Academy Of Sciences Of 

The Republic Of Uzbekistan 

 

A.D.Matchanov 
Tashkent Pharmaceutical Institute, Uzkfiti, Institute Of Bioorganic Chemistry Of The Academy Of Sciences Of 

The Republic Of Uzbekistan 

 

D.M.Sarvarova 
Tashkent Pharmaceutical Institute, Uzkfiti, Institute Of Bioorganic Chemistry Of The Academy Of Sciences Of 

The Republic Of Uzbekistan 

 

Journal Website: 

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Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

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Volume 04 Issue 01-2024 114 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 113-117 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

Chromatography makes it possible to carry out 

qualitative and quantitative analysis of the objects 

under study, study the physical and chemical 

properties of substances, and monitor and 

automatically regulate technological processes. Gas 

chromatography is a method for separating volatile 

compounds. 

This method can analyze gaseous, liquid and solid 

substances with a molecular weight of less than 400 

that satisfy certain requirements, the main ones being 

volatility, thermal stability, inertness and ease of 

preparation. Quantitative analysis can only be carried 

out if the substance is heat-resistant, i.e. evaporates 

reproducibly in the dispenser and elutes from the 

column without decomposition (1,7). 

 In pharmaceutical analysis, the GLC method is 

widely used to determine residual organic solvents in 

pharmaceutical substances, biological active 

substances in liquid and dry extracts of medicinal plant 

materials, as well as in assessing the quality of essential 

oils, volatile medicinal substances, and their 

preparations (2,5,6). 

 The purpose of our work is to study the 

quantitative content of biological active substances in 

a dry extract obtained from plant raw materials of 

saffron using gas-liquid chromatography. Previously, 

we studied the qualitative content of biologically active 

substances in plant raw materials of saffron using gas 

chromatography-mass spectroscopy (3). 

 For the quantitative determination of 

biologically active substances in the dry extract of 

saffron, we used the dry extract we had previously 

obtained by the percolation method (4). 

 

Experimental parts. 

Quantification method: 

Gas chromatograph – Khromatek-Kristall – 5000 (Russia) 

Detector-FID 

Detector temperature – 250 0С 

Thermostat temperature (gradient) - 70 0С (6min), 1500С (6-15min), 200 0С (15-33min), 220 0С (33-38min) 

Injector temperature - 200 0С 

Carrier gas – nitrogen 

Split flow – 1/20 

Injection – 1 µl 

 

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Volume 04 Issue 01-2024 115 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 113-117 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

Fig. 1 Chromatogram of standard samples 

Figure 1 shows chromatogram data for the three main standard substances isophorone with a retention time of 26.777 

min, 2,6,6-trimethylcyclohexa-1,3-diene-1-carbaldehyde with a column retention time of 28.783 min, and 4-

ochophorone with a retention time of 30.916 min . For comparison, the concentration of standard samples was 3 

mg/ml. 

 

Fig 2 Chromatogram of hexane extract of dry extract of Saffron 

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Volume 04 Issue 01-2024 116 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 113-117 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

From the data presented in Fig. 2, it is clear that in the hexane extract of the Saffron plant all three main components 

are observed: isophorone with a retention time of 26.766 minutes, 2,6,6-trimethylcyclohexa-1,3-dien-1-carbaldehyde 

with a retention time of column 28.751 min and 4-ochophorone with a retention time of 30.875 min. The quantitative 

content of isophorone is 0.76 mg/ml, 2,6,6-trimethylcyclohexa-1,3-diene-1-carbaldehyde is 5.71 mg/ml and 4-

ochophorone is 0.68 mg/ml. 

 

Fig 3 Chromatogram of acetonitrile extract of dry extract of Saffron 

 

From the data presented in Fig. 3, it is clear that in the 

acetonite extract of the Saffron plant two main 

components are observed: 2,6,6-trimethylcyclohexa-

1,3-diene-1-carbaldehyde with a retention time on the 

column of 28.745 minutes and 4-ochophorone with 

retention time 30.877 min. The quantitative content of 

2,6,6-trimethylcyclohexa-1,3-diene-1-carbaldehyde is 

0.44 mg/ml and 4-ochophorone is 0.42 mg/ml. 

Conclusion. Thus, studies have shown that for the 

extraction of Saffron it is relatively more effective to 

use hexane as a solvent. 

REFERENCES 

1. Е.А. Кривошеев, Р.И. Саяхов. «Газовая 

хроматография» Методические указания к 

лабораторной работе Казань, 2021. 23 с.  ВВ.  

2. В. Тыжигирова «Газовая 

хроматография»Краткая характеристика 

метода и его применение в 

фармацевтическом анализе  Учебное 

пособие Иркутск ИГМУ 2016 

3. М.О. Шомахсудова, А.А. Тулаганов, 

Я.К.Назирова, С.З. Нишанбаев. «Разработка 

https://doi.org/10.37547/ajbspi/Volume03Issue03-01
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Volume 04 Issue 01-2024 117 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 113-117 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

методов анализа биологически актывних 

веществ в растительном сыре шафрана 

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хромато-спектрометрии», Pharmaceutical 

journal,  №2, том.29, 2020 Тошкент. 

4. Шомаксудова М.О., Тулаганов А.А. .XI 

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конференции.  “Современная фармация 

новые падходы в образовании актуальные 

исследования ”,приуроченной к 20-летию 

факультета Фармация. 5май 2022й Нур-

Султан. 

5. Zuparova, Z. A., Olimov, N. K., Ismoilova, G. M., 

& Khasanova, B. J. (2020). Determination of 

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grown in Uzbekistan and the prospect of 

creating immunomodulatory medicinal 

products on its base. International Journal of 

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6. Zuparova, Z. A., and G. M. Ismoilova. 

"Determining The Authenticity Of Immunacea 

Bio Tablets With Immunomodulatory Action." 

Journal of Pharmaceutical Negative Results 

(2022): 2637-2641. 

7. Sarvarova, D. M., et al. "Study Of The 

Assortment Of Antioxidant And Hemostatic 

Medicines Registered In The Republic Of 

Uzbekistan." Journal of Pharmaceutical 

Negative Results (2023): 1028-1033. 

 

 

 

 

 

 

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