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American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 39-45 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
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ABSTRACT 

In practice, feed additives with various biological properties are used, such as biostimulants, modifiers, antioxidants, 

enzymes, phytobiotics, when fed to ruminants, an optimal rumen environment is created for microbial activity and 

digestion of food substrates in the diet. The research was to study biostimulants on milk production and the state of 

the immune system in cattle. 

KEYWORDS 

Feed additives, correction, immunostimulants, biostimulants, milk, livestock, leukocytes. 

INTRODUCTION

  Research Article 

 

COMPLEX EVALUATION OF BIOSTIMULANTS FOR PREVENTION OF 

IMMUNE SYSTEM DISORDERS AND HIGHLY PRODUCTIVE COWS AND 

IMPROVEMENT OF MILK QUALITY  
 

Submission Date: January 06, 2024, Accepted Date:  January 11, 2024,  

Published Date: January 16, 2024  

Crossref doi: https://doi.org/10.37547/ajbspi/Volume04Issue01-07 

 

 

Kakhorov B.A 
National University of Uzbekistan 

 

Rasulova S.L 
National University of Uzbekistan 

 

Zhumakulova G.S. 
National University of Uzbekistan 

 

Shavkatova H.R. 
National University of Uzbekistan 

 

 

Journal Website: 

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com/index.php/ajbspi 

Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

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Volume 04 Issue 01-2024 40 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 39-45 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
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Relevance. The intensification of cattle breeding and 

the use of industrial technologies significantly 

increases the load on the cow’s body and contributes 

to the strain of its functionality. Changing 

technological conditions of detention do not always 

correspond to the physiological needs of animals, and 

in this situation diseases arise, which are based on 

metabolic disorders. Among the complex of external 

conditions that affect the physical and chemical 

parameters of milk and their biological value, special 

attention should be paid to feeding dairy cattle. In 

addition, feed not only directly affects the productivity 

and quality of milk, but also indirectly affects the 

immune system. In recent years, in livestock farming, 

much attention has been paid to the development of a 

variety of additives that can increase milk productivity, 

milk fat content, increase the digestibility of feed and 

stimulate the metabolism of mineral and probiotic feed 

additives. In practice, feed additives with various 

biological properties are used, such as biostimulants, 

modifiers, antioxidants, enzymes, phytobiotics, when 

fed to ruminants, an optimal rumen environment is 

created for microbial activity and digestion of food 

substrates in the diet. Considering the above, an 

urgent problem is the creation and introduction into 

production of biostimulants intended for inclusion in 

mixed feeds and diets that have a beneficial effect on 

metabolism, animal productivity, the quality and safety 

of products of animal origin. 

Purpose and objectives of the research. The main goal 

of the study was to study biostimulants on milk 

production and the state of the immune system in 

cattle. 

To achieve the goal, the following tasks were set: 

1. Determine the effect of a feed biostimulant on the 

milk productivity of cows and the quality of raw milk. 

2. Study the biochemical parameters of cows’ blood 

when using a biostimulator. 

3. Assess the economic efficiency of introducing 

biostimulants into the diets of highly productive cows. 

 

METHODS 

Research was carried out using clinical-physiological, 

microbiological, veterinary-sanitary, zootechnical and 

mathematical methods. The effect of biostimulants in 

different doses on metabolic processes, productivity 

and quality of milk of cows was studied. The research 

was carried out on 32 dairy cows in the Zangiota district 

of the Tashkent region in 2021. Animals in the control 

and experimental groups received a diet consisting of 

wheat straw (0.6 kg), corn silage (23.0 kg), wheat 

haylage (50.0 kg), complete feed (14.58 kg), optigen (0 

.05 kg) and alfalfa haylage (6.50 kg). The animals of the 

experimental groups received a mineral feed regulator 

in a dose of 300 g daily in addition to the main diet (BR) 

as part of a complete feed. 

 

RESULTS AND DISCUSSION 

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Volume 04 Issue 01-2024 41 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 39-45 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
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In accordance with the accepted scheme for 

zootechnical analysis of feed, the dry matter of the 

feed is the carrier of the nutritional value of the feed. 

The higher the dry matter content of the feed, the 

higher its nutritional value. It is known that the DM 

consumption of bulky feeds depends on the 

concentration of metabolic energy in them and the 

level of productivity. Animals received biostimulants 

for 30 days, 10 ml. intramuscularly. Hematological 

parameters of the blood of experimental cows. As can 

be seen from the data presented in the table, over the 

entire period of the experiment, the concentration of 

leukocytes in the control and experimental groups was 

within physiological norms and had an average value of 

9.5 109/l in the control group and 9.9 109/l in the 

experimental groups. The content of lymphocytes at 

the beginning and at the end of the experiment in the 

control and experimental groups was within 

physiological norms and no significant changes were 

observed. Control group - average content for the 

entire period - 33.9%. Experienced groups: I – 27.4%; II –

27.8%; III – 29.01%. 

          Table 1 - Hematological parameters of the blood of experimental cows. 

 

Index 

Unit of 

measurement 

Groups 

Control 
Experienced 

I II III 

Beginning of the experiment 1st day (n=12). 

1 2 3 4 5 6 

Leukocytes 109/л 9,27±0,54  9,91±0,61  10,94±0,85  9,77±0,60 

Lymphocytes % 28,39±2,20  27,05±2,58  25,87±2,77  28,41±2,19 

Basophils % 10,57±0,67 9,17±0,57 11,18±1,75 9,41±0,82 

Granulocytes % 51,15±2,72 49,57±2,36 51,77±3,24 50,12±2,94 

Red blood 

cells 
1012/л 9,45±0,58 8,70±0,30 9,65±0,62 9,16±0,60 

Hemoglobin г/дл 13,79±1,60 10,25±0,23* 10,16*±0,21 13,39±1,67 

Thrombocytes 109/л 478,83±73,28 317,58±45,01 359,92±54,26 376,92±80,15 

End of experiment, 60th day (n =12) 

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Volume 04 Issue 01-2024 42 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 39-45 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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Leukocytes 109/л  9,84±0,58  9,02±0,43  9,93±0,57  9,78±0,56 

Lymphocytes % 29,51±2,02  27,84±1,96  29,80±2,07  29,62±2,14 

Basophils % 8,89±0,65  9,08±0,33  8,56±0,50  8,63±0,51 

Granulocytes % 50,13±2,34  44,56±1,74  44,87±1,76  44,15±1,68* 

Red blood 

cells 
1012/л 

8,66±0,28  8,08±0,25  8,16±0,22  8,12±0,22 

Hemoglobin г/дл 10,41±0,24  11,14±0,45  10,03±0,23  9,95±0,23 

Thrombocytes 109/л 293,25±41,72  340,46±46  295,42±38,76  286,17±36,30 

Note: * p<0.05; ** p<0.01; compared to control 

 

The ratio of basophils at the beginning of the 

experiment and at the end tended to decrease, namely 

in the control group it decreased by 15.9%. In the 

experimental groups it was 0.99%, 23.4 and 8.3%, 

respectively. All indicators were within physiological 

norms and no significant changes were observed. The 

content of granulocytes during the entire period of the 

experiment in comparison with the control in the 

experimental groups decreased by 11.2%, 10.5 and 11.9%, 

respectively, while being within physiological norms. 

At the beginning of the experiment, the content of the 

number of erythrocytes was within the maximum 

values of physiological norms (5-10*1012/l), at the end 

of the experiment it dropped to average values, 

namely in 

in the control group - 8.66 (1012 /l), in the experimental 

group - 8.12 (1012 /l). The hemoglobin indicator of the 

experimental groups at the beginning of the 

experiment was lower than the values of the control 

group: in I - by 25.7%; in II – by 26.3%; in III – by 2.9%. The 

indicators of the control group and experimental 

group III were higher than physiological norms at the 

beginning of the experiment. At the end of the 

experiment, all indicators were within physiological 

norms and had an average value for the control group 

- 10.41 g/dl and the experimental group - 10.37 g/dl. 

Hematocrit values for the entire period of the 

experiment were within physiological norms (35-45%) 

and no significant changes were observed. The 

average value was: control group – 39.48%; I – 35.5%; II 

– 40.38%; III – 38.72%. 

The number of platelets at the beginning of the 

experiment in the experimental groups was within 

physiological norms (250-450 1012/l), and the indicators 

in the control were higher. At the end of the 

experiment, all indicators were within physiological 

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Volume 04 Issue 01-2024 43 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 39-45 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
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norms and no significant changes were observed. Data 

on milk production (MP) of animals for 100 days of 

lactation were obtained on the basis of daily records 

during each milking. The study period was divided into 

5 additional periods of 20 days each. In general, 

research results have shown that the trend in the level 

of milk productivity is increasing. The subsequent 

increase is probably associated with the stabilization of 

the energy balance in the body of dairy cows. With the 

introduction of biostimulants in milk it increases, 

however, we did not observe an excess of the upper 

limit of the norm. In addition, it was found that in 

accordance with the selected analysis criteria in the 

studied population of animals, in 17.05% of cows the 

biostimulant corresponded to optimal values. The 

biostimulant tended to decrease as the milk 

productivity of animals increased, and increased as 

soon as the milk productivity of animals began to 

decline in the dynamics of milking days. The economic 

efficiency of feeding a biostimulant feed regulator in 

the diet of cows is the calculation of the economic 

feasibility of its use. The main indicators in the 

economic assessment were: the cost of feed, average 

daily milk yield and the market price of milk. 

 

CONCLUSION 

According to the task, further research was aimed at 

studying the effect of biostimulants on the 

productivity and metabolism of high-yielding cows. It is 

known that feeding, and as a consequence, the 

introduction of biostimulants, is one of the leading 

factors in ensuring high productivity of dairy livestock. 

This is explained by the fact that during lactation the 

animal’s body is in a state of increased functional 

activity; complex processes of fermentation of feed 

through a huge number of bacteria, fungi, protozoa, as 

well as the absorption of nutrients and the synthesis of 

new ones, take place in the proventriculus. All this 

provides the animal with the necessary energy and 

nutrients, affects the physiological processes 

occurring in the body, which in turn helps to enhance 

metabolic processes, productive and reproductive 

phenomena. An important point in these processes is 

the ability to control rumen digestion by adjusting the 

diet through the use of various biostimulants. 

 

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(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 39-45 

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OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 39-45 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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