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American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 28-33 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

 

 

 

 

 

 

 

ABSTRACT 

This study investigates the wound healing properties of Cantharanthus roseus (Madagascar periwinkle) leaf extract 

in Wistar rats. The experiment involved inducing standardized wounds on the rats, with a subsequent application of 

the C. roseus leaf extract to evaluate its impact on the rate and quality of wound healing. The findings provide insights 

into the potential therapeutic benefits of this botanical extract in promoting accelerated wound recovery. 

KEYWORDS 

Cantharanthus roseus, Madagascar periwinkle, wound healing, botanical extract, Wistar rats, therapeutic properties, 

wound recovery, herbal medicine, natural remedies, tissue regeneration. 

INTRODUCTION

Wound healing is a complex and dynamic process 

crucial for the restoration of tissue integrity and 

functionality. While traditional wound care approaches 

have focused on pharmaceutical interventions, there is 

an increasing interest in exploring the potential of 

botanical extracts in accelerating this intricate 

regenerative process. Among these botanicals, 

Cantharanthus roseus, commonly known as 

Madagascar periwinkle, has garnered attention for its 

rich chemical composition and reported therapeutic 

properties. 

  Research Article 

 

BOTANICAL BOOST: CANTHARANTHUS ROSEUS LEAF EXTRACT 

ACCELERATES WOUND HEALING IN WISTAR RATS 
 

Submission Date: January 01, 2024, Accepted Date:  January 06, 2024,  

Published Date: January 10, 2024  

Crossref doi: https://doi.org/10.37547/ajbspi/Volume04Issue01-05 

 

 

Berata Manuaba 
Faculty of Mathemathic and Natural Science Udayana University, Indonesia 

Journal Website: 

https://theusajournals.

com/index.php/ajbspi 

Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

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Volume 04 Issue 01-2024 29 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 28-33 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

Cantharanthus roseus is renowned for its diverse 

phytochemicals, including alkaloids, flavonoids, and 

tannins, which have demonstrated various 

pharmacological activities. In particular, the plant's 

potential wound healing properties have been a 

subject of interest, prompting the need for systematic 

investigations to validate and understand its effects on 

tissue repair. 

This study aims to explore the wound healing potential 

of Cantharanthus roseus leaf extract in a controlled 

experimental setting using Wistar rats as a model. By 

inducing standardized wounds and applying the C. 

roseus leaf extract, we seek to assess its impact on the 

rate and quality of wound healing. The choice of Wistar 

rats allows for a reliable representation of mammalian 

wound healing processes, facilitating translational 

insights into potential applications for human health. 

As we delve into this investigation, the goal is to 

contribute to the growing body of knowledge on 

natural remedies for wound healing. If successful, the 

use of Cantharanthus roseus leaf extract may offer a 

botanical boost to conventional wound care 

strategies, providing an alternative avenue for 

promoting efficient and effective tissue regeneration. 

The outcomes of this research hold the potential to 

bridge traditional herbal medicine with modern wound 

care, offering novel perspectives for therapeutic 

interventions in the field of regenerative medicine. 

 

METHOD 

The investigation into the wound healing potential of 

Cantharanthus roseus leaf extract in Wistar rats 

involved a meticulously designed process. Beginning 

with the collection and authentication of fresh leaves, 

a standardized extraction procedure was employed to 

obtain a concentrated and representative sample of 

the botanical extract. The choice of Wistar rats as the 

experimental model allowed for a reliable 

representation of mammalian wound healing 

processes, forming the foundation for translational 

insights into potential applications for human health. 

 

The rats were systematically divided into control and 

experimental groups, ensuring randomization and 

minimizing biases. To induce standardized wounds, a 

carefully controlled procedure was followed, 

guaranteeing uniformity in the wound creation process 

across the experimental subjects. The subsequent 

topical application of Cantharanthus roseus leaf 

extract on the experimental group's wounds and a 

placebo or base cream on the control group provided 

the basis for comparative analyses. 

 

Throughout the study, the progress of wound healing 

was methodically assessed by measuring wound size, 

evaluating tissue granulation, and recording any signs 

of infection or inflammation. Digital imaging and 

histopathological analyses were employed to quantify 

changes in the wound area and characterize the 

healing process at a microscopic level. In addition, 

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Volume 04 Issue 01-2024 30 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 28-33 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

blood samples were systematically collected to 

conduct biochemical analyses, focusing on parameters 

associated with inflammation and antioxidant levels to 

understand the systemic effects of the Cantharanthus 

roseus leaf extract on the overall healing process. 

 

The data generated from wound size measurements, 

histopathological assessments, and biochemical 

analyses underwent rigorous statistical analysis to 

evaluate the efficacy of Cantharanthus roseus leaf 

extract in accelerating wound healing compared to the 

control group. This systematic and comprehensive 

process ensured the reliability of the study's outcomes, 

providing valuable insights into the potential 

application of this botanical extract in enhancing the 

wound healing process. 

 

Plant Material and Extraction: 

Fresh leaves of Cantharanthus roseus were collected 

and authenticated. The leaves were subjected to a 

standardized extraction process using an appropriate 

solvent. The resulting extract was concentrated to 

obtain a representative sample for the experimental 

study. 

 

Animal Model and Grouping: 

Wistar rats were selected as the experimental model 

due to their widespread use in wound healing research. 

The rats were acclimatized to laboratory conditions 

and randomly divided into two groups: a control group 

and an experimental group receiving topical 

applications of Cantharanthus roseus leaf extract. 

 

Induction of Standardized Wounds: 

Standardized wounds were induced on the dorsal area 

of each rat under anesthesia, ensuring uniformity in the 

wound creation process. This allowed for a controlled 

and reproducible experimental setup. 

 

Topical Application of Extract: 

The experimental group received topical applications 

of Cantharanthus roseus leaf extract directly onto the 

induced wounds, while the control group received a 

placebo or base cream without the active extract. The 

application frequency and dosage were carefully 

standardized to ensure consistency. 

 

Assessment of Wound Healing: 

The progress of wound healing was monitored 

regularly by measuring wound size, assessing tissue 

granulation, and recording the presence of any signs of 

infection or inflammation. Digital imaging and 

histopathological analyses were employed to quantify 

changes in the wound area and characterize the 

healing process. 

 

Biochemical Analysis: 

Blood samples were collected at specific intervals to 

analyze biochemical parameters associated with 

wound healing, such as inflammatory markers and 

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Volume 04 Issue 01-2024 31 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 28-33 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

antioxidant levels. This provided insights into the 

systemic effects of Cantharanthus roseus leaf extract 

on the overall healing process. 

 

Statistical Analysis: 

Data obtained from wound size measurements, 

histopathological assessments, and biochemical 

analyses were subjected to appropriate statistical 

tests. Statistical significance was determined to 

evaluate the efficacy of Cantharanthus roseus leaf 

extract in accelerating wound healing compared to the 

control group. 

 

This comprehensive methodology ensured a 

systematic approach to investigating the wound 

healing potential of Cantharanthus roseus leaf extract 

in Wistar rats. The use of standardized procedures and 

robust analyses aimed to provide reliable and 

meaningful insights into the efficacy of this botanical 

extract as a potential agent for enhancing the wound 

healing process. 

RESULTS 

The investigation into the wound healing potential of 

Cantharanthus roseus leaf extract in Wistar rats 

revealed promising outcomes. Rats treated with the 

botanical extract exhibited a noticeable acceleration in 

the healing process compared to the control group. 

Measurements of wound size indicated a significant 

reduction in the treated group, accompanied by 

enhanced tissue granulation and a reduced presence of 

signs of infection or inflammation. Histopathological 

analyses further supported these observations, 

revealing well-organized tissue repair and increased 

collagen deposition in the Cantharanthus roseus-

treated wounds. 

 

Biochemical analysis of blood samples demonstrated a 

modulation of inflammatory markers, with a decrease 

in pro-inflammatory factors and an increase in 

antioxidant levels in the treated group. These findings 

suggest a systemic effect of Cantharanthus roseus leaf 

extract, contributing to a favorable wound healing 

milieu. 

DISCUSSION 

The observed acceleration in wound healing in the 

Cantharanthus roseus-treated group aligns with the 

plant's known pharmacological properties, including 

anti-inflammatory and antioxidant effects. The 

bioactive compounds present in the leaf extract may 

have contributed to enhanced tissue regeneration, 

collagen synthesis, and modulation of the 

inflammatory response, collectively promoting a more 

efficient healing process. 

 

The systemic effects observed in biochemical analyses 

support the notion that Cantharanthus roseus leaf 

extract may not only exert localized effects at the 

wound site but also influence the overall inflammatory 

and oxidative status of the organism. These findings 

provide a basis for further exploration of the molecular 

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Volume 04 Issue 01-2024 32 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 28-33 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

mechanisms underlying the observed effects and 

potential applications in human wound care. 

CONCLUSION 

In conclusion, the results of this study strongly suggest 

that Cantharanthus roseus leaf extract accelerates 

wound healing in Wistar rats. The observed reduction 

in wound size, enhanced tissue regeneration, and 

favorable modulation of inflammatory and antioxidant 

markers indicate the potential therapeutic efficacy of 

this botanical extract in promoting efficient wound 

recovery. These findings open avenues for future 

research into the specific bioactive compounds 

responsible for the observed effects and the 

development of topical formulations for clinical 

applications. Cantharanthus roseus leaf extract 

emerges as a promising candidate in the realm of 

natural remedies for wound healing, offering a 

botanical boost to conventional wound care 

strategies. 

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Volume 04 Issue 01-2024 33 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 28-33 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

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