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American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 22-27 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

 

 

 

 

 

 

 

 

 

ABSTRACT 

Using light microscopy methods, the histological structure of the uterus of white outbred rats at the level of the body 

and cervix was clarified. It has been established that the walls of the right and left uterine horns grow together, 

forming a double body and neck. Whose cavities remain separated from each other by a thin septum and open into 

the vagina with two separate openings. The area of fusion of the medial walls is characterized by the union of the 

perimeter and the supravascular layer of the myometrium. The data obtained can be used to illuminate the relevant 

sections of reference and educational literature when writing sections on evolutionary, functional, comparative 

species morphology and reproductive biology. Based on the histological study, it was established that in rats, despite 

the general type of uterine structure, there are significant differences that mainly affect their lower segment at the 

borders. 

KEYWORDS 

Uterus, cervix, white outbred rats, myometrium, smooth myocyte. 

INTRODUCTION

  Research Article 

 

MORPHOLOGICAL CHARACTERISTICS OF THE UTERUS OF WHITE 

OUTBRED RATS 
 

Submission Date: January 01, 2024, Accepted Date:  January 05, 2024,  

Published Date: January 08, 2024  

Crossref doi: https://doi.org/10.37547/ajbspi/Volume04Issue01-04 

 

 

Ishankulova Dildora Xabibullayevna 
Bukhara State Medical Institute, Uzbekistan 

 

Ixtiyarova Gulchehra Akmalovna 
Bukhara State Medical Institute, Uzbekistan 

 

Ilyasov Aziz Saidmuratovich 
Bukhara State Medical Institute, Uzbekistan 

 

Journal Website: 

https://theusajournals.

com/index.php/ajbspi 

Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

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Volume 04 Issue 01-2024 23 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 22-27 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

The study of animal templates, the development of 

animals and the methods of their management, their 

reproduction processes, are some of the pressing 

problems of the time. Basic knowledge shows the 

structure of laboratory animals, and we can 

understand the mechanisms of differentiation, 

abnormal histo - and Organogenesis, as well as 

contribute to greater depth and purpose. A better 

understanding of morphological bases, relationships 

between tissues during development, renewal, and 

restoration can help study multi-tissue systems. From 

laboratory animals, due to the onset of the most 

favorable and rapid puberty for a century and a half, 

the short duration of embryogenesis and the short 

period of postpartum stretching involution are rats and 

rabbits.  The presence of animal material was achieved 

to ensure the purity of the samples. An analysis of the 

literature shows that there are many unanswered 

questions related to the rest the structure of some 

organs and organ systems is new, in particular, it 

concerns the genital area; that is, the uterus is the 

sections of its caudal parts associated with the 

formation of the body and neck at the level of 

insertion. There are also many unresolved problems, 

related to the structure of its caudal part, and 

especially its appearance at the microscopic level. The 

smooth muscle is still not well understood. Myometrial 

tissue in the caudal part of the uterus, as you know, is 

characterized by originality. What functions it 

performs and does it to the maximum extent during 

endocrine dependence, pregnancy and childbirth. The 

practical significance of this information can be useful 

for biologists, veterinarians and, perhaps, 

Obstetricians-Gynecologists, I think. 

Taking into account the above, the purpose and 

comparative analysis of the study, with the 

histostructural Organization of the uterine wall of the 

rat, its caudal part was taxied in modern histo-

cytological methods. 

Research objective. Expanding knowledge of the 

morphological structure of the female genital organs, 

in particular the uterus in rats. 

MATERIAL AND METHODS 

The work used 15 bats ranging from 4 months to 1 year. 

The uterus was used as an object of study. The Material 

was ivitized to a 10% buffered formalin solution. The 

placement of the material was carried out on a 

histological closed-type vacuum Leica ASP 300 

processor. The material used Histomix paraffin from 

the BioOpticamiz firm. The cuts were made in a rotor 

Microtome 6-7 µm thick. The work used methods of 

light microscopy, painting cuts with hematoxylin and 

eosin dye. Immunogystochemical testing of caudal 

tissue was also performed. The ratio of the uterine 

section to actin in smooth muscles was carried out 

using a set of monoclonal antitanours. The 

establishment of an immunogystochemical reaction 

was achieved with a one-step imaging system. 

Results and analyzes. The squid uterus contains a pair 

of horns, body and neck, and the uterine horns join in a 

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Volume 04 Issue 01-2024 24 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 22-27 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

caudal direction to form the body and neck, but their 

cavities are separated by a barrier and open with two 

independent holes in the vagina. It should be noted 

that in basic education, veterinary and monographic 

applications in development biology in rats, according 

to the basic classification of bacadon, the uterus is 

defined as bipartite, that is, the Union of bodies to 

form a single cervical hole. With complex study, this 

question becomes clear that the uterus should be 

classified as two-horned the uterus of rats. 

A histological examination found that the wall of the 

branches, body and cervix has a similar structure and 

consists of three walls: endometrium (mucous 

membrane), myometrium (muscle) and perimetry 

(serosis). 

 

(Figure 1). Horn and glands of the uterus of a white broodless rat.  Coloring: with hematoxylin and eosin. 

The mucous membrane forms a relief in the form of folds that become more pronounced on the neck (fig. 2). The 

formed endometrium forms in two layers (plates): epithelial and specific. 

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Volume 04 Issue 01-2024 25 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 22-27 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

(fig. 3) the cervix and glands of a white broodless rat. Coloring: with Van Gison. 

 

(fig. 4) the cervix and glands of a white broodless rat. Coloring: with Van Gison. 

Thus, in the lower segment, a median border is formed 

that separates the uterus from the two body and 

cervical cavities, which are vaguely expressed. The 

middle border is made up of endometrium and 

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Volume 04 Issue 01-2024 26 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 22-27 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

myometrium. At the same time, myometrium has 

submucosal and single vascular layers in its structure. 

The vascular layer is more expressed in the body. 

 

CONCLUSIONS 

Histo-morphological studies conducted are considered 

important, and its results can be used in reference and 

educational literature and reproduction biology when 

writing evolutionary, functional, relative species 

morphology sections to write relevant sections. This 

may allow a comparative study of the structure of the 

female genitalia and the observation of their 

homology. In the development of various mammals, 

people, revealing their evolutionary dynamics, is of 

great importance. Thus, in rats, the cervix has more in 

common with humans, which reveals in advance what 

should be considered when looking for an 

experimental model when conducting research. 

REFERENCES 

1. Глаголев, П. А. Анатомия сельскохозяйственных 

животных с основами гистологии и эмбриологии 

/ П. А. Глаголев, В. И. Ипполитова. – 1977. – 450 c. 

2. Григорьева, Ю. В. Особенности строения 

миометрия нижнего сегмента матки 

лабораторных крыс / Ю. В. Григорьева, Н. В. 

Ямщиков, А. В. Бормотов, К. Ф. Гарифуллина // 

Фундаментальные исследования. – 2012. – № 12-1. 

– С. 48–51. 

3. Григорьева, Ю. В. Морфологическая 

характеристика миоцитов миометрия матки 

крыс при беременности и родах / Ю. В. 

Григорьева, Н. В. Ямщиков, Н. А. Ренц, А. В. 

Бормотов // Фундаментальные исследования. – 

2013. – № 12-2. – С. 195–199. 

4. Кладовщиков, В. Ф. Стимулировать развитие 

нутриеводства и кролиководства / В. Ф. 

Кладовщиков, В. Н. Александров 

Кролиководство и нутриеводство. – 2002. – № 3. 

– С. 19–20. 

5. Малакшинова, Л. М. Гистологическая и 

гистохимическая характеристика матки 

крольчих Л. М. Малакшинова // Состояние и 

перспективы развития агропромышленного 

комплекса Забайкалья: 

6. Материалы научно-практической конференции 

(4–6 февраля 2003 г.). – Бурятская ГСХА им. В. Р. 

Филиппова. – Улан-Удэ, 2003. – С. 82–86. 

7. Ноздрачев, А. Д. Анатомия крысы 

(лабораторные животные) / А. Д. Ноздрачев, Е. 

Л. Поляков ; под ред. проф. А. Д. Ноздрачева. – 

СПб. : Лань, 2001. – 464 с. 

8. Савинов, П. А. Разработка экспериментальной 

модели эндометриоза, адаптированной к 

современным хирургическим технологиям / П. 

А. Савинов, Д. А. Ниаури, Н. В. Ковшова // Вестник 

Санкт-Петербургского университета, 2006. – 

Сер. 11. – Вып. 3. – С. 114–119. 

9. Томитова, Е. А. Гистоструктура, 

гистохимические и морфологические 

показатели слизистой матки, шейки матки и 

https://doi.org/10.37547/ajbspi/Volume03Issue03-01
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Volume 04 Issue 01-2024 27 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 01 PAGES: 22-27 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

влагалища крольчих в норме и под 

воздействием экзогенных половых гормонов / Е. 

А. Томитова // Актуальные аспекты 

экологической, сравнительно-видовой, 

возрастной и экспериментальной морфологии: 

Материалы международной 

научнопрактической конференции, 

посвященной 100-летию профессора Вениамина 

Яковлевича Суетина (24–27 июня 2004 г.) / ФГОУ 

ВПО «Бурятская ГСХА им. В. Р. Филиппова», фак. 

вет. медицины. – Улан-Удэ, 2004. – С. 186–191. 

10. Хрусталева, И. В. Анатомия домашних животных 

: учебник / И. В. Хрусталева, Н. В. Михайлов, Я. И. 

Шнейберг и др. – изд. 3-е, испр. – М. : Колос, 

2006. –704 с. 

11. Ишанкулова Д.Х, Ильясов А.С, Ихтиярова Г.А. 

Воздействие энергетических напитков на 

репродуктивную систему человека и 

животных//Тиббиётда янги кун.-2023.5(55) 341-

344 ст. 

12. Ишанкулова Д.Х, Ильясов А.С, Ихтиярова Г.А, 

Ишанкулова Ш.А. Анализ потребления 

энергетического напитка с кофеином среди 

подростков Бухарской области//Ветеринария 

медитцинаси.-2023. Махсус сон.121-122 б.  

13. 10. Bulat, R. Studies of the innervation of rabbit 

myometrium and cervix / R. Bulat, M. S. Kannan, R. 

E. Garfield // Can J Physiol Pharmacol. – 1989. – Aug; 

67(8):837–44. PubMed PMID: 2598120. 

14. Chilton, Beverly S. Isolation and characterization of 

rabbit endocervical cells / Beverly s. Chilton, Santo 

V. Nicosia, Janice M. Sowinski and Don P. Wolf // 

The journal of cell biology. – Volume 86. – July 1980. 

– P. 172–180. 

15. 12. Odor, D. L. Light and electron microscopic 

observation on the cervical epithelium of the rabbit 

/D. L. Odor, R. J. Blandau //Amer. J. Anat. – 1988. – 

V. 181. – № 3. – P. 289–319.

 

 

 

 

 

 

 

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