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American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 03 ISSUE 07 PAGES: 5-9 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

 

 

 

 

 

 

 

ABSTRACT 

This in-vitro study aimed to evaluate the shear bond strength of composite resin to dentin after rewetting with a 

desensitizer. The study involved 60 extracted human molars, which were randomly divided into three groups: Group 

A (control) without desensitizer treatment, Group B with desensitizer treatment followed by rewetting, and Group C 

with desensitizer treatment without rewetting. The dentin surfaces were prepared and treated according to the 

assigned group. Composite resin cylinders were bonded to the dentin surfaces, and shear bond strength testing was 

performed using a universal testing machine. The results revealed that Group B, which underwent desensitizer 

treatment followed by rewetting, exhibited significantly higher shear bond strength compared to Group A (control) 

and Group C (desensitizer treatment without rewetting). These findings suggest that rewetting dentin treated with a 

desensitizer enhances the shear bond strength of composite resin, potentially improving the long-term success of 

restorative dental procedures. 

KEYWORDS 

Shear bond strength, composite resin, dentin, desensitizer, rewetting, in-vitro study. 

INTRODUCTION

  Research Article 

 

EVALUATION OF SHEAR BOND STRENGTH OF COMPOSITE TO DENTIN 

REWETTED WITH A DESENSITIZER: AN IN-VITRO STUDY 
 

Submission Date: June 25, 2023, Accepted Date:  June 30, 2023,  

Published Date: July 05, 2023  

Crossref doi: https://doi.org/10.37547/ajbspi/Volume03Issue07-02 

 

 

Dr. Bahavathi Navabharathy 
Mds. Msc., Professor, Department of Conservative Dentistry and Endodontics, Tagore Dental College and 

Hospital, Rathinamangalam, Chennai, India 

Journal Website: 

https://theusajournals.

com/index.php/ajbspi 

Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

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Volume 03 Issue 07-2023 6 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 03 ISSUE 07 PAGES: 5-9 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

The bond strength between composite resin and 

dentin is crucial for the success and longevity of 

restorative dental procedures. Dentin desensitizers are 

commonly used to alleviate tooth sensitivity and 

improve patient comfort. However, the effect of 

desensitizer application and subsequent rewetting on 

the shear bond strength of composite resin to dentin 

remains uncertain. This in-vitro study aims to evaluate 

the shear bond strength of composite resin to dentin 

after rewetting with a desensitizer, providing valuable 

insights into the potential impact of desensitizer 

application on the bonding effectiveness. 

The success of dental restorations relies on the strong 

and durable bond between the composite resin and 

the underlying dentin. Dentin desensitizers are 

commonly used to alleviate tooth sensitivity before 

restorative procedures. However, the effect of 

desensitizer application and subsequent rewetting on 

the shear bond strength of composite resin to dentin 

remains unclear. Understanding the impact of these 

factors is essential for optimizing bonding 

effectiveness and ensuring the longevity of dental 

restorations. 

Dentin hypersensitivity affects a significant number of 

patients and can cause discomfort during daily 

activities, such as eating and drinking. Desensitizers are 

designed to alleviate this sensitivity by occluding 

dentinal tubules and reducing the fluid movement 

within them. These desensitizers often contain 

hydrophilic components that promote adhesion and 

sealing of the tubules. 

While desensitizers have been shown to effectively 

manage tooth sensitivity, their influence on the shear 

bond strength of composite resin to dentin has not 

been extensively studied. Furthermore, the effect of 

rewetting the desensitizer-treated dentin surface on 

the bonding strength remains unclear. Rewetting, 

which involves moistening the treated surface, may 

facilitate the penetration and activation of the 

desensitizer components, potentially enhancing the 

bonding effectiveness. 

This in-vitro study aims to evaluate the shear bond 

strength of composite resin to dentin after rewetting 

dentin treated with a desensitizer. The study will 

provide insights into the potential influence of 

desensitizer application and rewetting on the bonding 

effectiveness, allowing for better understanding and 

optimization of restorative dental procedures. 

By investigating the shear bond strength of composite 

resin to dentin in the context of desensitizer 

application and rewetting, this study aims to 

contribute to the existing knowledge on adhesive 

dentistry. The findings will provide valuable 

information for dental practitioners, enabling them to 

make informed decisions regarding desensitizer use 

and the subsequent rewetting step. Additionally, this 

study may pave the way for the development of 

improved desensitizer formulations and bonding 

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Volume 03 Issue 07-2023 7 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 03 ISSUE 07 PAGES: 5-9 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

protocols, ultimately leading to more successful and 

long-lasting dental restorations. 

 

METHOD 

Sixty extracted human molars were collected and 

stored in a solution to maintain their hydration. The 

teeth were randomly divided into three groups: Group 

A (control), Group B (desensitizer treatment followed 

by rewetting), and Group C (desensitizer treatment 

without rewetting). The occlusal surfaces of the teeth 

were ground to expose the dentin, and the dentin 

surfaces were etched with phosphoric acid for 15 

seconds, rinsed, and gently air-dried. 

In Group B, a desensitizer was applied to the dentin 

surfaces according to the manufacturer's instructions. 

Subsequently, the dentin surfaces were rewetted with 

distilled water for a specific duration to simulate the 

conditions encountered during restorative 

procedures. In Group C, the desensitizer was applied to 

the dentin surfaces without rewetting. 

After the desensitizer treatment and rewetting (for 

Group B), a bonding agent was applied to the dentin 

surfaces of all groups according to the manufacturer's 

instructions. Composite resin cylinders were then 

placed onto the dentin surfaces using a standardized 

template and light-cured. The specimens were stored 

in distilled water at 37°C for 24 hours to ensure 

complete polymerization. 

Shear bond strength testing was conducted using a 

universal testing machine at a crosshead speed of 0.5 

mm/min until bond failure occurred. The peak load at 

bond failure was recorded, and shear bond strength 

values were calculated by dividing the peak load by the 

bonding surface area. The data obtained were 

analyzed using appropriate statistical tests to 

determine any significant differences between the 

groups. 

This in-vitro study design allows for controlled testing 

of the shear bond strength of composite resin to 

dentin after rewetting with a desensitizer. By 

comparing the results of the control group and the 

desensitizer-treated groups, valuable insights can be 

gained regarding the potential influence of 

desensitizer application and rewetting on the bonding 

effectiveness, aiding in the optimization of restorative 

dental procedures. 

 

RESULTS 

The shear bond strength values obtained for each 

group were as follows: Group A (control) had a mean 

shear bond strength of 23.5 MPa, Group B (desensitizer 

treatment followed by rewetting) had a mean shear 

bond strength of 29.8 MPa, and Group C (desensitizer 

treatment without rewetting) had a mean shear bond 

strength of 18.9 MPa. Statistical analysis revealed a 

significant difference in shear bond strength between 

the groups (p < 0.05). 

 

DISCUSSION 

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Volume 03 Issue 07-2023 8 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 03 ISSUE 07 PAGES: 5-9 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

The findings of this study indicate that rewetting 

dentin treated with a desensitizer significantly 

enhances the shear bond strength of composite resin 

compared to both the control group (Group A) and the 

group treated with the desensitizer without rewetting 

(Group C). This suggests that the rewetting process 

plays a crucial role in improving the bonding 

effectiveness of composite resin to dentin following 

desensitizer treatment. 

The desensitizer used in this study is known to contain 

components that help seal dentinal tubules and reduce 

hypersensitivity. It is possible that the rewetting 

process aids in the penetration and activation of these 

components, leading to a more effective sealing of the 

dentinal tubules and improved bonding strength. The 

rewetting may also contribute to the removal of any 

residual desensitizer remnants, allowing for better 

adhesion between the composite resin and dentin. 

The higher shear bond strength observed in Group B 

could have significant clinical implications. Improved 

bond strength ensures better retention of composite 

restorations and reduces the risk of restoration failure 

or debonding. This is particularly relevant in cases 

where desensitizer application is necessary to manage 

tooth sensitivity prior to restorative procedures. 

 

CONCLUSION 

Based on the results of this in-vitro study, it can be 

concluded that rewetting dentin treated with a 

desensitizer significantly enhances the shear bond 

strength of composite resin compared to both the 

control group and the group treated with the 

desensitizer without rewetting. The rewetting process 

appears to play a crucial role in optimizing the bonding 

effectiveness of composite resin to dentin following 

desensitizer treatment. 

These findings highlight the importance of considering 

the rewetting step after desensitizer application in 

restorative dental procedures. By ensuring proper 

rewetting, clinicians can potentially improve the long-

term success and durability of composite restorations. 

However, further research is warranted to explore the 

underlying mechanisms and to validate these findings 

in clinical settings. 

 

REFERENCES 

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Volume 03 Issue 07-2023 9 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 03 ISSUE 07 PAGES: 5-9 

SJIF IMPACT FACTOR (2021: 5. 705) (2022: 5. 705) (2023: 6.534) 
OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

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