







































Volume 04 Issue 09-2024 54 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 09 PAGES: 54-57 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

 

 

 

 

 

 

 

 

ABSTRACT 

Dysbiosis is a condition characterized by the disruption of the normal ratio of microorganisms in the body, which can 

lead to various physiological disorders. This study investigates the biochemical changes in the saliva of rats under 

dysbiosis induced by antibiotics. A comparative analysis of the control and experimental groups revealed significant 

alterations in the levels of albumin, immunoglobulin IgA, electrolytes, and organic acids. The results indicate that 

dysbiosis leads to an increase in the levels of albumin and electrolytes, as well as a decrease in immunoglobulin IgA, 

suggesting potential impairments in immune response and metabolism. The findings underscore the importance of 

maintaining normal microbiota for health and may serve as a foundation for further research in the prevention and 

treatment of dysbiosis. 

KEYWORDS 

Dysbacteriosis, Saliva, Rats, Biochemical changes, Albumin, ImmunoglobulinIgA, Electrolytes, Organic acids, 

Antibiotics, Microbiota, Digestion, Immune response, Inflammation, Metabolism, Homeostasis. 

INTRODUCTION

  Research Article 

 

BIOCHEMICAL CHARACTERISTICS OF RAT SALIVA UNDER CONDITIONS 

OF DYSBACTERIOSIS 
 

Submission Date: Sep 20, 2024, Accepted Date:  Sep 25, 2024,  

Published Date: Sep 30, 2024  

Crossref doi: https://doi.org/10.37547/ajbspi/Volume04Issue09-05 

 

 

Malikov Ilkhom Rishodovich 
Associate Professor of Medicine, University of Alfraganus, Uzbekistan 

 

Abdukarimov Dilshod Isakovich 
Senior Lecturer, Department of Histology and Medical Biology, TGSI, Uzbekistan 

 

 

 

 

Journal Website: 

https://theusajournals.

com/index.php/ajbspi 

Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

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Volume 04 Issue 09-2024 55 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 09 PAGES: 54-57 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

Dysbacteriosis is a condition characterized by a 

disruption of the normal ratio of microorganisms in the 

body, which can lead to various physiological 

disorders. This condition is most often associated with 

a change in the composition of the intestinal 

microflora, which negatively affects metabolism and 

immune function. Saliva plays an important role in the 

processes of digestion and protection of the body, as 

it contains enzymes, antibodies and other components 

responsible for maintaining homeostasis. Studying 

biochemical changes in the composition of saliva in rats 

with dysbacteriosis can provide important data on the 

mechanisms underlying this condition, as well as its 

impact on the overall health of animals. 

Purpose of the study 

The aim of this study is to investigate the biochemical 

changes in the saliva composition of rats with 

antibiotic-induced dysbacteriosis. The study aims to 

identify changes in the levels of key salivary 

components such as albumin, immunoglobulin IgA , 

electrolytes and organic acids, and to understand the 

impact of dysbacteriosis on physiological functions of 

the body. 

METHODS 

Wistar rats were used for the experiment, divided into 

two groups: control and experimental. The 

experimental group received ampicillin for two weeks, 

which led to the suppression of normal microflora. 

Saliva was collected using a non-invasive method 

based on stimulation of salivation. Spectrophotometry 

and chromatography were used for analysis, allowing 

the concentration of proteins, electrolytes and organic 

acids to be determined. 

RESULTS AND DISCUSSION 

The results of the study showed significant changes in 

the biochemical composition of the saliva of rats with 

dysbacteriosis. 

1. Changes in protein levels: 

Albumin concentration increased by 30% in the 

experimental group compared to the control. This may 

indicate inflammatory processes and the body's 

systemic response to microbiota disruption. The level 

of immunoglobulin IgA decreased by 40%, indicating a 

decrease in the local immune response and potential 

vulnerability to infections. 

2. Electrolyte composition: 

Sodium and potassium levels increased by 25% in the 

experimental group, which may reflect changes in 

water and electrolyte balance. Calcium levels 

decreased by 15%, which may be associated with 

metabolic and intestinal dysfunction. 

3. Organic acids: 

Lactate levels increased by 20%, which may indicate 

metabolic changes and increased glycolysis under 

stress conditions. These changes in saliva composition 

may have a significant impact on digestion and the 

body's defenses. 

 

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Volume 04 Issue 09-2024 56 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 09 PAGES: 54-57 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

Table 1 

Indicator Control group Experimental group Change (%) 

Albumin level 

(g/L) 

30 39 +30% 

Immunoglobulin 

IgA (g/l) 

1.5 0.9 -40% 

Sodium ( mM ) 140 175 +25% 

Potassium ( mM ) 4.5 5.6 +25% 

Calcium ( mM ) 2.5 2.1 -15% 

Lactate ( mM ) 1.2 1.4 +20% 

 

The obtained results show that dysbacteriosis causes 

significant biochemical changes in the composition of 

rat saliva. Increased levels of albumin and electrolytes 

may be associated with activation of inflammatory 

systems and stress reactions. A decrease in IgA 

indicates a weakening of the local immune response, 

which may increase the risks of infectious diseases and 

aggravate the consequences of dysbacteriosis. 

It is also worth noting that changes in the level of 

organic acids, such as lactate, may indicate a metabolic 

disorder associated with changes in the microbiota. 

Lactate is a product of anaerobic metabolism and can 

accumulate under conditions of hypoxia or other 

metabolic disorders. 

It should also be mentioned that saliva has many 

functions, including participation in the initial stages of 

digestion, protection of teeth and mucous 

membranes, and participation in the immune 

response. Changes in the composition of saliva may 

indicate the need for the body to adapt to new 

conditions and may be important markers of the health 

of animals. 

CONCLUSION 

The study revealed significant biochemical changes in 

the composition of rat saliva during dysbacteriosis. 

These changes emphasize the importance of normal 

microflora for maintaining health and homeostasis of 

the body. The data obtained may be useful for 

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Volume 04 Issue 09-2024 57 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 09 PAGES: 54-57 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

developing methods for the prevention and treatment 

of dysbacteriosis in both animals and humans. Further 

research is needed to more fully understand the 

mechanisms underlying dysbacteriosis and its impact 

on various physiological systems. 

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