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American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 12 PAGES: 18-21 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

 

 

 

 

 

 

 

 

 

 

ABSTRACT 

This article investigates the biochemical characteristics of guinea pig saliva under conditions of dysbiosis. Dysbiosis, 

which represents a disruption of normal microbiota, can significantly impact the health of animals and their 

physiological processes. In the course of the experiment, key components of saliva, including proteins, carbohydrates, 

and electrolytes, were analyzed to identify changes associated with microbiota imbalance. The results showed 

significant deviations in the biochemical composition of guinea pig saliva, indicating potential mechanisms by which 

dysbiosis affects digestion and immune response. These findings emphasize the importance of monitoring microbiota 

status and biochemical indicators of saliva for maintaining the health of guinea pigs. 

KEYWORDS 

Dysbacteriosis, guinea pigs, saliva, biochemical characteristics, microflora, animal health, physiological processes. 

INTRODUCTION

  Research Article 

 

CHANGES IN THE COMPOSITION OF GUINEA PIG SALIVA DURING 

MICROBIOTA IMBALANCE 
 

Submission Date: December 01, 2024, Accepted Date:  December 06, 2024,  

Published Date: December 11, 2024  

Crossref doi: https://doi.org/10.37547/ajbspi/Volume04Issue12-04 

 

 

Babakulov Sharof Khamrokulovich 
Associate Professor of the Department of Surgery and Military Field Surgery, TGSI, Uzbekistan 

 

Babakulova Shakhlo Khamidullaevna 
Assistant of the Department of Public Health, Healthcare Management and Physical Culture of TGSI, 

Uzbekistan 

 

Abdukarimov Dilshod Isakovich 
Senior Lecturer, Department of Histology and Medical Biology, TGSI, Uzbekistan 

 

 

 

 

Journal Website: 

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com/index.php/ajbspi 

Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

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Volume 04 Issue 12-2024 19 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 12 PAGES: 18-21 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

Dysbacteriosis is a condition characterized by a 

disruption of the normal balance of microbiota in the 

body. This phenomenon can occur under the influence 

of various factors, such as poor nutrition, stress, 

infectious diseases or the use of antibiotics. In guinea 

pigs, as in other animals, dysbacteriosis can lead to 

serious disturbances in the digestive system and 

weakened immune defense. 

Saliva plays a key role in the digestive process and in 

protecting the mucous membranes of the oral cavity. 

It contains many biochemical components, including 

enzymes, proteins and antibodies, which help maintain 

the health of the body. Studying changes in the 

biochemical composition of saliva in dysbacteriosis can 

provide valuable information about the health of 

guinea pigs and the mechanisms that contribute to the 

development of diseases. 

Purpose of the study 

The aim of this study is to analyze the biochemical 

characteristics of guinea pig saliva under conditions of 

dysbacteriosis, to identify the main changes and their 

potential impact on animal health. 

METHODS 

The study involved 30 guinea pigs, aged 8-12 weeks. 

The animals were divided into two groups: a control 

group (15 guinea pigs, without signs of dysbacteriosis) 

and an experimental group (15 guinea pigs, in which 

signs of dysbacteriosis were established). Guinea pigs 

were kept in standard conditions, with access to clean 

water and balanced feed. All animals were vaccinated 

and clinically examined before the experiment. 

Saliva was collected by encouraging animals to chew 

soft food for 15 minutes. The collected material was 

placed in sterile containers and stored at -20°C until 

analysis. 

Analysis of biochemical characteristics of saliva was 

carried out using the following methods: 

• Determination of protein levels: Biuret test 

method. 

• Measurement of carbohydrate content: 

Phenolic test method. 

• Electrolyte analysis: Ion chromatography. 

• Measurement of lysozyme activity: 

Colorimetric method. 

The obtained data were processed using a statistical 

software package. Differences between groups were 

analyzed using a t-test, with a significance level of p < 

0.05. 

RESULTS AND DISCUSSION 

The study revealed significant changes in the 

biochemical composition of guinea pig saliva under 

conditions of dysbacteriosis. 

1. Protein level 

o In the experimental group, the protein level in 

saliva averaged 2.5 g/ dL , which was significantly 

higher than in the control group (1.8 g/ dL ) (p < 0.05 ). 

2. Carbohydrate content 

o The carbohydrate content in the saliva of 

guinea pigs with dysbacteriosis increased to 0.6 g/ dl , 

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Volume 04 Issue 12-2024 20 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 12 PAGES: 18-21 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

compared with 0.4 g/ dl in the control group (p < 0.05 

). 

3. Electrolyte concentration 

o Salivary sodium and potassium levels also 

showed significant differences: sodium was 140 mM /L 

in the experimental group versus 120 mM /L in the 

control group (p < 0.01 ), while potassium levels 

increased from 4.0 mM /L to 5.2 mM /L (p < 0.05). 

4. Lysozyme activity 

o The activity of lysozyme in the saliva of guinea 

pigs with dysbacteriosis was 0.25 μg/ml, which is lower 

than in the control group, where this indicator was 0.35 

μg/ml (p < 0.05 ). 

All the changes identified were statistically significant, 

confirming the impact of dysbacteriosis on the 

biochemical characteristics of guinea pig saliva. The 

results highlight the importance of saliva as an 

indicator of animal health and the potential impact of 

dysbacteriosis on their physiological functions. 

CONCLUSION 

A study of the biochemical characteristics of guinea pig 

saliva under conditions of dysbacteriosis showed 

significant changes in the composition of saliva 

compared to the control group. An increase in protein 

and carbohydrate levels, as well as a change in the 

concentration of electrolytes, indicate disturbances in 

physiological processes in the animals' bodies. A 

decrease in lysozyme activity indicates a possible 

weakening of the immune defense. 

The obtained data highlight the importance of 

monitoring the microflora status and biochemical 

parameters of saliva for the early detection of 

dysbacteriosis and other diseases. This may help in 

developing effective prevention and treatment 

strategies aimed at maintaining the health of guinea 

pigs. 

Further research is needed to better understand the 

mechanisms underlying changes in the biochemical 

composition of saliva and to assess the impact of 

various factors on the health of guinea pigs. 

REFERENCES 

1. Chekarova I.A. Comparative morphology of th 

parotid salivary gland female male castrate pigs 

/I.A. Chekarova agricultural science bulletin-2007-

№1.- P. 68-71. // Siberian 

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parotid, mandibular, sublingual and zygomatic 

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Volume 04 Issue 12-2024 21 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 12 PAGES: 18-21 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

domestic and wild animals (domestic pig, wild wild 

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glands in immature rats: morphometric and 

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