







































Volume 04 Issue 12-2024 14 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 12 PAGES: 14-17 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

ABSTRACT 

The aim of this study is to analyze the stages of morphological development of the parotid salivary glands of rabbits 

during postnatal ontogenesis. The paper considers the key stages of differentiation and formation of these glands, as 

well as their morphofunctional features at different stages of postnatal development. Using histological and 

morphometric analysis, changes in the structure of the glands, as well as their relationship with the development of 

other organs of the digestive system, were studied. The results demonstrate significant changes in the morphology 

of the parotid salivary glands of rabbits, which occur at different stages of their development. Particular attention is 

paid to age-related differences in the cellular structure and functional activity of the glands, which is important for 

further studies of the physiology and pathology of the salivary glands in animals. This study contributes to the 

understanding of the biological mechanisms of salivary gland formation in mammals. 

  Research Article 

 

STAGES OF MORPHOLOGICAL DEVELOPMENT OF THE PAROTID 

SALIVARY GLANDS OF RABBITS IN POSTNATAL ONTOGENESIS 
 

Submission Date: December 01, 2024, Accepted Date:  December 06, 2024,  

Published Date: December 11, 2024  

Crossref doi: https://doi.org/10.37547/ajbspi/Volume04Issue12-03 

 

 

Chinniyeva M.I. 
Elder Professor, Department of Histology and Medical Biology, TGSI, Uzbekistan 

 

Melibayev Orif Yunusovich 
Professor of the Tashkent State Dental Institute, Uzbekistan 

 

Nabiyeva Z.B. 
Elder Professor, Department of Histology and Medical Biology, TGSI, Uzbekistan 

 

Rixsiyeva M.A. 
Assistant of the Department of Histology and Medical Biology, TGSI, Uzbekistan 

 

Soatova M.S. 
Assistant of the Department of Histology and Medical Biology, TGSI, Uzbekistan 

 

 

 

Journal Website: 

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Copyright: Original 

content from this work 

may be used under the 

terms of the creative 

commons attributes 

4.0 licence. 

 

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Volume 04 Issue 12-2024 15 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 12 PAGES: 14-17 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

KEYWORDS 

Parotid salivary glands, rabbits, postnatal ontogenesis, morphological development, morphogenesis, salivary glands, 

differentiation, histology, morphofunctional features, cell differentiation. 

INTRODUCTION

The salivary glands play an important role in the body 

of mammals, participating in the processes of 

digestion, moistening the oral cavity and protection 

from pathogenic microorganisms. The parotid salivary 

glands, in particular, are one of the largest paired 

glands that secrete a secretion containing enzymes, 

amylose and other substances necessary for digestion 

of food. These glands, like other exocrine organs, 

undergo a complex process of morphological and 

functional development during ontogenesis, which is 

important for understanding not only their normal 

function, but also the pathology associated with 

disturbances in their development. In rabbits, as one of 

the common objects of zoological research, the 

parotid salivary glands play an important role in 

digestion, and their morphological development in the 

postnatal period is of particular interest for studying 

the mechanism of formation and differentiation of 

glandular tissues. However, despite the importance of 

these studies, issues related to the morphogenesis of 

the parotid salivary glands in rabbits in postnatal 

ontogenesis remain insufficiently covered in the 

scientific literature. The present study is aimed at 

studying the morphological features of the 

development of the parotid salivary glands in rabbits in 

the postnatal period. The main attention is paid to the 

various stages of their development, as well as the 

analysis of morphofunctional changes during growth 

and formation. The data obtained can serve as a basis 

for further research in both normal physiology and 

pathology of the salivary glands, which, in turn, is 

important for veterinary medicine and biology. 

The purpose of the study  

The aim of this study is to analyze the stages of 

morphological development of the parotid salivary 

glands in rabbits during postnatal ontogenesis, with an 

emphasis on the key stages of cell differentiation, the 

development of gland structures and their 

morphofunctional changes at different age stages. 

METHODS  

The study involved the use of rabbit carcasses of 

various age groups, from newborns to adults. The 

animals were obtained from a laboratory nursery and 

were used in accordance with the ethical standards for 

scientific research. 

Morphological examination of the parotid salivary 

glands was performed using histological methods. For 

this purpose, tissue samples were selected, fixed in 10% 

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Volume 04 Issue 12-2024 16 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 12 PAGES: 14-17 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

formalin solution and then processed with paraffin. 

Sections 5-7 μm thick were stained with hematoxylin 

and eosin to assess the general morphology of the 

glands, as well as using special methods (for example, 

staining according to the Verhoeff method) to 

highlight the structural features of the cells and ducts. 

To evaluate the morphometric parameters, a light 

microscope imaging technique was used with a digital 

camera and appropriate software. Cell sizes, duct wall 

thicknesses, cell density in different areas of the glands 

were measured, and changes in tissue structure were 

analyzed depending on the age of the animals. 

In addition, to identify functional changes in the 

structure of the salivary glands, immunohistochemical 

analysis was performed using antibodies to specific 

markers of cells involved in the production of saliva. 

RESULTS AND DISCUSSION  

Morphological examination of the parotid salivary 

glands showed that with age, rabbits experience 

significant development of the structure of these 

glands. In newborn animals, the glands were less 

differentiated, with insufficiently developed ducts and 

an insignificant number of secretory cells. While in 

adult rabbits, clear differentiation of cells, developed 

ducts and pronounced secretory activity are observed. 

The use of hematoxylin and eosin made it possible to 

isolate mature acinar cells in older individuals, 

indicating the development of functional maturity of 

the glands. While in newborns, immature cells with less 

pronounced secretory activity predominated. 

Morphometric analysis showed that the size of the 

cells and the thickness of the duct walls increase with 

age. In newborn rabbits, the cells of the parotid glands 

were significantly smaller in size, and the walls of the 

ducts were thinner. While in adult animals, the cell sizes 

increased and the duct walls thickened, indicating an 

increase in the functional activity of the glands. The cell 

density also increased with age, which may be due to 

the intensity of saliva secretion and the adaptation of 

the glands to a more active function. 

Immunohistochemical analysis using antibodies to 

markers of cells involved in saliva production (e.g., 

amylose and acid mucoprotein) showed that in adult 

rabbits the expression level of these markers was 

significantly higher than in neonates. This confirms the 

hypothesis that with age, the activity of the salivary 

glands and their ability to produce saliva increases. 

In newborn individuals, markers of saliva production 

were poorly expressed, which may be associated with 

insufficient maturity of the salivary glands at this age. 

CONCLUSION 

The obtained data indicate that the parotid salivary 

glands of rabbits undergo significant changes during 

the process of growth and development. From 

newborn to adult individuals, both morphological and 

functional changes occur, which correlate with the age 

and level of development of the animal. 

The increase in cell size and thickening of the duct walls 

is associated with an increase in the functional activity 

of the glands, which may be due to the body's need for 

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Volume 04 Issue 12-2024 17 

                 

 
 

   
  
 

American Journal Of Biomedical Science & Pharmaceutical Innovation    
(ISSN – 2771-2753) 
VOLUME 04 ISSUE 12 PAGES: 14-17 

OCLC – 1121105677     

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Publisher: Oscar Publishing Services 

Servi 

a larger volume of saliva secretion. Increased 

expression of saliva production markers in adult 

rabbits confirms that with age, the salivary glands 

become more active and specialized. 

These results can serve as a basis for further research 

aimed at studying changes in the salivary glands in 

other animal species or humans, as well as for 

developing methods to improve the functioning of the 

salivary glands in various diseases. 

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