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 VOLUME Vol.05 Issue04 2025 

PAGE NO. 27-32 

DOI 10.37547/ajbspi/Volume05Issue04-07 

 
 
 
 

Using Bidens And Achillea To Treat Acute Finger Bowel 

Ulcers and Researching Its Phenolic Characteristics 
 

 Ibragim Askarov 

Andijan State University, Andijan, Uzbekistan 

 

 Khabibullo Kodirov 

Andijan Medical Institute, Andijan, Uzbekistan 

 

 

Received: 24 February 2025; Accepted: 20 March 2025; Published: 23 April 2025 

 

Abstract: One of the most prevalent gastrointestinal disorders is duodenal ulcers. Approximately 5–10% of people 
worldwide will at some point in their lives suffer with this illness. There are two to three times as many males as 
females. Between the ages of 30 and 50, more develops. Disease rates are high in industrialized nations like 
Uzbekistan, the US, and Europe. Although the illness is very uncommon in Asia and Africa, Helicobacter pylori 
infections are quite common in places with inadequate sanitation. Ninety-five percent of cases are caused by 
Helicobacter pylori infection. Fast food that is hot, salty, and greasy causes malnutrition. Nervousness and 
psychological aspects. Bidens and Achillea are used to treat duodenal ulcers. When it comes to gastrointestinal 
disorders, the herbs Bidens and Achillea are said to be quite beneficial. They have antibacterial, anti-inflammatory, 
and wound-finishing properties. A solution containing 14.286 mg/L of each vitamin was created after the first 200 
µL of B1, B6, B3, B12, and PP were removed. This led to the creation of standard solutions with 7.143, 3.571, and 
1.786 mg/l. Standard vitamin C solutions with concentrations of 286, 143, 71.5, and 57.2 mg/l were also prepared. 
Pure water with a concentration of 0 mg/l was used to construct a calibration graph. 

 

Keywords: Gastrointestinal disorders, Helicobacter pylori infections. 

 

Introduction: In Uzbekistan, duodenal ulcers are 
among the most prevalent gastrointestinal conditions. 
Approximately 5% of people have this illness[10]. The 
condition is more prevalent in people between the ages 
of 20 and 40. It is five to six times more common in men 
than in women. Risk factors: An poor diet that is 
inconsistent might cause the condition to develop. The 
development of the condition is influenced by stress 
and psychoemotional elements, including mental 
weariness and both acute and chronic mental events. 
There are certain distinct symptoms associated with 
duodenal ulcer illness, even if they are similar to those 
of stomach ulcers[13]. These are its primary symptoms: 
Pain in the upper abdomen, just above the navel, is the 
most common symptom. "Hunger pains" are made 
worse by prolonged fasting or starvation [4,8]. 

After eating, it feels better. It may show up early in the 

morning or at night. Due to the overproduction of 
gastric juice, boiling the crust (increased acidity) would 
result in a bitter aftertaste behind the chest. It 
frequently happens after hot and fatty meals. 
collection of gas and stomach bloating. abdominal 
swelling and pain following a meal. Flatulence, or gas 
buildup, is seen as a result of the intestinal system's 
malfunction[9]. 

Some people experience nausea after eating. In 
extreme situations, excessive production of stomach 
juice results in vomiting. Some individuals experience 
an increase in appetite; they eat more to quell "hunger 
pains." Conversely, in some, weight loss and a 
reduction in appetite are seen. Constipation or 
constipation due to poor intestinal function might be 
noted. Severe consequences, such as bleeding or a 
deeper cut. vomiting that is dark or crimson (stomach 

 

https://doi.org/10.37547/ajbspi/Volume05Issue04-07
https://doi.org/10.37547/ajbspi/Volume05Issue04-07
https://orcid.org/0000-0003-1625-0330
https://orcid.org/0000-0001-6574-7576


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American Journal of Applied Science and Technology (ISSN: 2771-2745) 
 

 

hemorrhage). Feces that resemble black (blood mixed 
feces) [5]. Acute, stabbing pain is a sign of a perforation, 
or puncture of the wound. Emergency medical 
attention is required if the patient goes into a condition 
of shock! It is advised to consult a physician if the illness 
is exhibiting symptoms! Maintaining a healthy lifestyle 
and safeguarding the gastrointestinal tract are crucial 
in preventing duodenal ulcers.  

The following actions lower the chance of contracting 
the illness: a well-organized diet: eating at the same 
time each day will stop the stomach from producing too 
much liquid. Consuming meals that are easy to digest, 
such as milk and dairy products (if not tolerated). 
steamed veggies and meat. cereal-topped porridge 
(oatmeal, buckwheat). Avoid meals that are fried, salty, 
spicy, or very hot or cold[11]. Avoidance of strong 
coffee, alcohol, and carbonated beverages. The 
stomach lining can be harmed by ongoing stress, thus 
relaxation and following a sleep schedule (sleep for at 
least 7-8 hours) are important. Increasing physical 
activity (mild walking, sports), practicing yoga, 
meditation, or breathing techniques. defense against 
infection with Helicobacter pylori. hand washing before 
to eating. consuming only wholesome, properly 
prepared meals. adherence to personal hygiene 
regulations. using caution when taking medicine. 
avoiding long-term use of medications that might harm 
the stomach, such as aspirin, ibuprofen, and non-
steroidal anti-inflammatory medicines, unless 
prescribed by a physician. It is advised to take these 
medications with stomach protectors if they must be 
taken. Getting rid of negative habits. Smoking tobacco 
and consuming alcohol damage the gastric mucosa and 
increase the risk of developing a wound. Doctor 
examination. Regular visits to the doctor if you have 
digestive problems or chronic gastritis. Check for 
Helicobacter pylori bacteria if necessary. Duodenal 
ulcer with medicinal herbs davolash.An in traditional 
medicine, various medicinal plants are used to treat 
duodenal ulcers [15]. These plants help protect the 
gastric mucosa, reduce inflammation, and repair 
damaged tissue. The best plants and ways to use them 
are listed below: Bidens's effects include covering the 
stomach mucosa, protecting it, and lowering 
inflammation. It has an anti-inflammatory action, heals 
wounds fast, and repairs the stomach mucosa. One 
glass of boiling water must be used to soak one 
spoonful of Bidens baggi and rose, and the mixture 
must be allowed to brew for ten to fifteen minutes. It is 
advised to consume it on an empty stomach each 
morning.                                                       .  

Course: two to three weeks.  

Dalachoy (Hypericum perforatum). Effect: preserves 
the mucous membrane, balances the acidic 

environment of stomach juice, and speeds up wound 
healing. It reduces inflammation and has a relaxing 
impact on spasms. Use instructions: Infuse one glass of 
boiling water with one spoonful of dried dalachoy for 
half an hour. Drinking one-third cup before meals two 
to three times a day is advised. Other advice: before 
consuming therapeutic herbs, a doctor should be 
contacted. It's critical to maintain a balanced diet and 
stay away from fried, spicy, and salty meals. Stress 
should be avoided, and restful sleep should be 
prioritized. These natural and efficient techniques are 
utilized as adjuvant therapy in conjunction with 
prescription drugs[3,13].  Bidens and Achilleaare used 
to treat duodenal ulcers. When it comes to 
gastrointestinal disorders, the herbs Bidens and 
Achillea are said to be quite beneficial. They have 
antibacterial, anti-inflammatory, and wound-finishing 
properties. Bidens's beneficial qualities include 
restoring the stomach juice's equilibrium. lessens 
inflammation. For half an hour, soak one teaspoon of 
dried Bidens in one cup of hot water. Two to three 
times a day, half a glass should be consumed before 
meals. The beneficial qualities of 
Achillea(tysjachelistnik, Achillea millefolium) include its 
antibacterial and anti-inflammatory effects. restores 
and protects the stomach mucosa. 

regulates the overproduction of gastric juice. Infuse 1 
cup of hot water with 1 tablespoon of dried Achilleafor 
20 minutes. Two to three times a day, half a glass 
should be consumed before meals. A blend of 
Achilleaand Bidens. When combined, these herbs have 
a more potent effect on the digestive tract. Soak one 
teaspoon each of Achilleaand Bidens in one cup of hot 
water, then let it sit for half an hour [2]. Two to three 
times a day, you can have half a glass before meals. 

Before using these plants over an extended period of 
time, it is advised to speak with a physician. Care should 
be used when using Bidens in people who have low 
stomach acid. It is not advised for youngsters or 
pregnant women to consume these plants. Duodenal 
ulcers can also be treated with the use of natural 
healing agents like Achilleaand Bidens. Assessment of 
the extract's water-soluble vitamin content using the 
YUSSCH method Experience section. used equipment 
and a jet. Rhydburg Pharmaceuticals (Germany), Carl 
Roth GmbH (Germany), DSM Nutritional Products 
GmbH (Germany), B1, B2, B3, B6, and PP vitamins 
(BLDPharm) are the sources of vitamin B12. The 
following reagents were utilized at HPLC level purity: 
water, acetonitrile, chemically pure brand acetic acid, 
and sodium hydroxide.  

The high-performance liquid chromatograph LC-40 
Nexera Lite, manufactured by the Japanese company 
Shimadzu, was used to measure the quantity of water-



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American Journal of Applied Science and Technology (ISSN: 2771-2745) 
 

 

soluble vitamins present in the plant [1]. standard 
solutions' preparation. C (CAS 50-81-7), B1 (CAS 59-43-
8), B6 (CAS 58-56-0), B3 (CAS 59-67-6), B12 (CAS 68-19-
9), and PP (CAS 98-92-0) solutions are made by 
dissolving 50 milliliters of 0.1 n li HCl solution 

containing 5 milligrams of each vitamin (100 mg/l). 
Vitamins B2 (CAS 83-88-5) and B9 (CAS 59-30-3) were 
dissolved in 50 milliliters of a 0.025% sodium hydroxide 
solution to create standard solutions[9].  

Table 1: Vitamin identification using a phase gradient technique. 

 

Time, minutes Atsetonitril (A), % 0.5% acetic acid (B), % 

0 0 100 

3 0 100 

14 20 80 

17 50 50 

18 0 100 

25 Finish 

 

Table 2: Vitamin C levels are determined using the motile phase gradient software. 

 

Time, minutes Atsetonitril (A), % 0.5% acetic acid (B), % 

0 0 100 

2 0 100 

6 50 50 

6,01 0 100 

15 Finish 

 

 
1. Fig.. Chromatogram of a standard solution of vitamins. 

 



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American Journal of Applied Science and Technology (ISSN: 2771-2745) 
 

 

 
2. Fig. Vitamin C standard solution chromatogram. 

 

RESULTS  

Determination of vitamins in the sample extract. A 
chromatogram of the sample extract (3-4 pictures) was 
taken, and on the basis of the results, the amounts of 
vitamins contained in 100 g of the sample were 
calculated with the following formula and presented in 
Tables 3. 

𝑋 =
𝐶𝑣𝑖𝑡 ∙ 𝑉𝑒𝑘𝑠𝑡𝑟𝑎𝑐𝑡

𝑚𝑠𝑎𝑚𝑝𝑙𝑒
∙ 100 𝑔 

Here, the content of vitamins in the fruit of x – 100 
grams, mg; 

C vit is the concentration of the vitamin contained in 
the extract determined by the Yussch method,mg/l; 

V ekstract-sample extract size, l; 

Sample is the mass of a sample weighed to make an 
extract. 

 

 
3. Fig. Chromatogram for the determination of vitamins in the sample extract. 



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American Journal of Applied Science and Technology (ISSN: 2771-2745) 
 

 

 
4. Fig Chromatogram for the determination of vitamins in the sample extract. 

 

Table 3. 

The amount of vitamins in the extract and the capture Times. 

Vitamin 

Capture time, 

SEC  

 

concentration, mg 

/ l 

100 g sample 

quantity, mg 

Vitamin B1 2,927 7,576 18,940 

Vitamin B3 5,776 19,361 48,403 

Vitamin PP 7,608 303,107 757,768 

Vitamin B9 16,981 0,445 1,113 

Vitamin B2 18,853 1,044 2,610 

Vitamin B6 6,544 0,704 1,760 

Vitamin B12 Aniqlanmadi 0 0,000 

Vitamin C 4,088 2,958 7,395 

After removing the first 200 µL of B1, B6, B3, B12, and 
PP from the vitamins, a solution was made with a 
14.286 mg/L concentration of each vitamin. Standard 
solutions containing 7.143, 3.571, and 1.786 mg/l were 
thus made. Additionally, standard vitamin C solutions 
containing 286, 143, 71.5, and 57.2 mg/l were made. A 
calibration graph was created using pure water at a 
concentration of 0 mg/l. 

Getting the sample extract ready. One gram of the 
material under examination was put in a 50 ml conical 
flask, and 25 ml of 0.1 n HCL solution was added in 
order to extract the water-soluble vitamins. The 
mixture was extracted for 20 minutes at 60 oC in an 
ultrasonic bath bearing the GT SONIC-D3 (Chinese) 
trademark. After cooling and filtering, the mixture was 
transferred to a measuring flask containing 25 
milliliters of water. The extract was utilized for analysis 
after being filtered and vialed in a 1.5 ml 0.22 µm 
syringe filter. conditions for chromatography. Vitamin 
determination. The LC-40d pump, SIL-40 autocamples, 
SPD-M40 photo-diode matrix detector (PDA), LC-40 
Nexera Lite high-performance liquid chromatograph, 
and LabSolutions version include the standard 

solutions and sample extract. Software version 6.92 
was used to examine it. Both a gradient motile phase 
and a reverse phase colon of Shim pack GIST C18 (150 
× 4.6 mm; 5 µm, Shimadzu, Japan) were used (Table 1).  

A gradient motile phase (Table 1) comprising 
acetonitrile (A) and 0.25% li solution (B) of acetic acid 
in water was applied, along with a reverse phase Colon 
of Shim pack GIST C18 (150 × 4.6 mm; 5 µm, Shimadzu, 
Japan). The column thermostat was set to 40 °C, the 
injection volume was set to 10 MCL, and the flow rate 
was set to 0.6 ml/min. Three wavelengths (265, 291, 
and 550 nm) were used to record each vitamin's 
analytical signal (peak area) (1-3 photos) [12,14]. 
Vitamin C was determined using a gradient that lasted 
15 minutes (Table 2), and the analytical signal was 
measured at a wavelength of 265 nm. 

CONCLUSION  

In Uzbekistan, duodenal ulcers are prevalent and 
primarily affect young males. The condition can be 
avoided by maintaining a healthy diet, managing stress, 
and treating Helicobacter pylori infections as soon as 
possible. One of the most prevalent gastrointestinal 
conditions in Uzbekistan is the epidemiology of 



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American Journal of Applied Science and Technology (ISSN: 2771-2745) 
 

 

duodenal ulcers. Approximately 5% of people have this 
illness.  Distribution by gender and age. Most people 
with the condition are between the ages of 20 and 40. 
It is five to six times more common in men than in 
women.  

In Uzbekistan, duodenal ulcers are prevalent and 
primarily affect young males. The condition can be 
avoided by maintaining a healthy diet, managing stress, 
and treating Helicobacter pylori infections as soon as 
possible. A gastroenterologist should be consulted if 
any of the aforementioned symptoms are seen. 
Complications can be avoided with early identification 
and treatment. 

There may be a duodenal ulcer if discomfort starts at 
night or when you're hungry and goes away after you 
eat. Stomach ulcers are likely to occur if the patient has 
nausea, weight loss, and increased discomfort after 
eating. Early identification and treatment are crucial 
since stomach ulcers may be linked to an increased risk 
of cancer. The chance of getting duodenal ulcers can be 
decreased by maintaining a healthy lifestyle, eating a 
balanced diet, and managing stress. In addition to 
preventing the wound, prophylactic adherence will 
enhance general health. 

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