American Journal Of Biomedical Science & Pharmaceutical Innovation 19 https://theusajournals.com/index.php/ajbspi VOLUME Vol.05 Issue06 2025 PAGE NO. 19-24 DOI 10.37547/ajbspi/Volume05Issue06-05 Technology for Obtaining the Biologically Active Supplement “Cardioherb” Ishonkulova N.F Tashkent Scientific Research Institute of Vaccines and Serums, Uzbekistan Mahmudjanova K.S. "ALFRAGANUS UNIVERSITY" non-governmental higher education organization, Uzbekistan Ashurov A.A. Tashkent Scientific Research Institute of Vaccines and Serums, Uzbekistan Received: 22 April 2025; Accepted: 18 May 2025; Published: 20 June 2025 Abstract: Currently, cardiovascular diseases are the leading cause of death and disability among humans [1,2]. In this regard, the development of herbal medicines not only for treatment but also for prevention is considered highly relevant. Dietary supplements (biologically active food additives or BAFAs) are mainly used as preventive agents [3]. Keywords: Biologically active, preventive agents, scientific research. Introduction: It is well known that for a long time, plant-based medications such as hawthorn and Geranium collinum have proven to be effective cardioprotective agents [4]. These plants have been widely used not only in folk medicine but also in scientific research. Numerous studies indicate that these plants are rich in various biologically active compounds, making them highly valuable in medicine. Research Objects. The research utilized hawthorn fruits (Crataegus) and roots/rhizomes of Geranium collinum, which grow in the Republic of Uzbekistan. Research Objective. The literature contains data on obtaining extracts from hawthorn fruits and the underground parts of Geranium collinum [5]. However, despite numerous studies, no combined phytopreparation from these two plants currently exists. The goal of our research is to develop a technology for obtaining extracts from hawthorn fruits and Geranium collinum roots and rhizomes, and on this basis, to produce a dietary supplement in capsule form named “Cardioherb.” Research Results. During the development of extraction technology, several factors were considered: degree of raw material grinding, type of extractant, extraction method, chemical composition, etc. Hawthorn fruits were ground into sizes of 1–2 mm, 3– 5 mm, and 7–8 mm. Extracts were obtained using maceration, repercolation, and the method of VNIIF (All-Union Scientific Research Institute of Pharmacy). The content of flavonoids—dihydroquercetin, luteolin, rutin, rosavin, quercetin, salidroside—was determined in the extracts using HPLC (High Performance Liquid Chromatography) (see Figure 1). https://doi.org/10.37547/ajbspi/Volume05Issue06-05 https://doi.org/10.37547/ajbspi/Volume05Issue06-05 https://doi.org/10.37547/ajbspi/Volume05Issue06-05 https://doi.org/10.37547/ajbspi/Volume05Issue06-05 American Journal of Applied Science and Technology 20 https://theusajournals.com/index.php/ajast American Journal of Applied Science and Technology (ISSN: 2771-2745) Figure 1. Flavonoid content in hawthorn extracts Ethanol at different concentrations (30%, 50%, 70%) was used as the extractant (Tables 1– 3). Table 1. Content of extractive substances in hawthorn extract (maceration method) Article size (mm) Flavonoids Ethanol concentration, % 30 50 70 Flavonoid content mg/g 1-2 Dihydroquercetin 0,012 0,017 0,015 Luteolin 0,0098 0,012 0,010 Rutin 0,037 0,041 0,035 Rosavin 0,113 0,123 0,121 Quercetin 0,0095 0,011 0,010 Salidroside 1,62 1,65 1,58 3-5 Dihydroquercetin 0,017 0,018 0,0186 Luteolin 0,012 0,013 0,0131 Rutin 0,041 0,045 0,0453 Rosavin 0,123 0,125 0,126 Quercetin 0,011 0,013 0,0137 Salidroside 1,65 1,68 1,68 7-8 Dihydroquercetin 0,016 0,015 0,012 Luteolin 0,011 0,010 0,0098 Rutin 0,039 0,035 0,037 Rosavin 0,122 0,121 0,113 Quercetin 0,011 0,010 0,0095 Salidroside 1,62 1,58 1,62 American Journal of Applied Science and Technology 21 https://theusajournals.com/index.php/ajast American Journal of Applied Science and Technology (ISSN: 2771-2745) Table 2. Content of extractive substances in hawthorn extract (repercolation method) Article size (mm) Flavonoids Ethanol concentration, % 30 50 70 Flavonoid content mg/g 1-2 Dihydroquercetin 0,012 0,017 0,015 Luteolin 0,0098 0,011 0,010 Rutin 0,037 0,041 0,032 Rosavin 0,112 0,123 0,121 Quercetin 0,0095 0,011 0,010 Salidroside 1,62 1,65 1,59 3-5 Dihydroquercetin 0,017 0,018 0,018 Luteolin 0,012 0,013 0,013 Rutin 0,041 0,043 0,049 Rosavin 0,121 0,125 0,126 Quercetin 0,011 0,013 0,013 Salidroside 1,65 1,69 1,69 7-8 Dihydroquercetin 0,016 0,015 0,012 Luteolin 0,011 0,010 0,0098 Rutin 0,038 0,035 0,036 Rosavin 0,112 0,121 0,113 Quercetin 0,011 0,010 0,0093 Salidroside 1,62 1,58 1,54 Table 3. Content of extractive substances in hawthorn extract (VNIIF method) Аrticle size (mm) Flavonoids Ethanol concentration, % 30 50 70 Flavonoid content mg/g 1-2 Dihydroquercetin 0,015 0,016 0,008 Luteolin 0,012 0,011 0,0005 Rutin 0,041 0,03 0,016 Rosavin 0,113 0,11 0,095 Quercetin 0,011 0,012 0,0093 Salidroside 1,65 1,71 1,43 3-5 Dihydroquercetin 0,018 0,02 0,009 Luteolin 0,013 0,014 0,0005 Rutin 0,045 0,05 0,02 Rosavin 0,125 0,13 0,097 Quercetin 0,013 0,014 0,0095 Salidroside 1,68 1,77 1,45 American Journal of Applied Science and Technology 22 https://theusajournals.com/index.php/ajast American Journal of Applied Science and Technology (ISSN: 2771-2745) 7-8 Dihydroquercetin 0,013 0,014 0,008 Luteolin 0,011 0,009 0,0005 Rutin 0,038 0,02 0,02 Rosavin 0,098 0,11 0,094 Quercetin 0,087 0,011 0,0093 Salidroside 1,58 1,69 1,38 Tables 1 to 3 show the concentration of flavonoids in hawthorn extracts obtained via maceration, repercolation, and VNIIF methods, respectively. Experimental data show that the highest yield of active compounds was achieved using the VNIIF method. Optimal results were obtained using 3–5 mm particle size and 50–70% ethanol. For further research, 50% ethanol was chosen as the extractant. The second stage of the study focused on developing a technology for producing a dry extract from Geranium collinum roots and rhizomes. While previous studies (Z. Pazylbekova et al.) obtained such an extract using 40% ethanol and the percolation method [6], they did not use the VNIIF method. We applied the VNIIF method using 30%, 50%, and 70% ethanol (see Table 4). Table 4. Content of extractive substances in Geranium collinum extract (VNIIF method) Аrticle size (mm) Ethanol concentration, % 30 50 70 Gallic acid content, mkg/kg 3-5 721,64 771,74 765,84 Figure 2. Gallic acid content in Geranium collinum extract The data show that the extracts contain a high amount of bioactive substances when using 50% and 70% ethanol. Based on the experimental data and to accelerate extraction, we developed a technology for obtaining a complex extract using a 2:1 ratio of hawthorn to Geranium collinum. Both raw materials were ground to 3–5 mm, extracted via the VNIIF method with 50% ethanol, and the resulting dry extract was conditionally named “Cardioherb.” Further studies involved qualitative and quantitative analysis of the dry extract. The appearance, authenticity, moisture content, heavy metal concentration, and active substances were determined. Results are shown in Table 5. American Journal of Applied Science and Technology 23 https://theusajournals.com/index.php/ajast American Journal of Applied Science and Technology (ISSN: 2771-2745) Table 5. Quality characteristics of the dry extract “Cardioherb” Indicator Analysis Result Appearance Brown-colored extract with a distinct taste and odor Authenticity: Flavonoids 2 g of the dry extract is extracted with 20 ml of 70% ethanol for 20 minutes. After filtration, 2-3 drops of a 2% aluminum chloride solution are added to 2 ml of the extract. The appearance of yellow color indicates the presence of flavonoids. Tannins 1 g of the extract is heated and extracted in 10 ml of distilled water for 15 minutes at a temperature of 60 ° C. After filtering the extract, 3-5 drops of 1% ferric (III) chloride solution are added to 2 ml of the extract. The formation of a blue-black color indicates the presence of tannins. Moisture 4,01% Heavy metals, % 0,007% Active substances: Flavonoids, mg/g Dihydroquercetin 0,23 Luteolin 0,05 Rutin 13,76 Rosavin 26,3 Quercetin 0,097 Salidroside 11,62 Gallic acid, mkg/kg 1295,42 As can be seen from the data presented in Table 5, the dry extract of the complex composition "Cardiogerb" meets the requirements of the pharmacopoeia in all respects, and subsequently, on its basis, the technology of dietary supplements in the form of capsules was developed for use as a cardioprotective agent. CONCLUSION For the first time, a technology was developed to produce a complex dry extract named “Cardioherb”, intended as a cardioprotective agent for the prevention of cardiovascular diseases. REFERENCES Cardiovascular diseases. WHO Fact Sheet No. 310, 2015. Available: http://www.whogis.com/mediacentre/factsheets/fs31 7/ru/ Medik V.A. Public health and healthcare: a medico- http://www.whogis.com/mediacentre/factsheets/fs317/ru/ http://www.whogis.com/mediacentre/factsheets/fs317/ru/ http://www.whogis.com/mediacentre/factsheets/fs317/ru/ http://www.whogis.com/mediacentre/factsheets/fs317/ru/ American Journal of Applied Science and Technology 24 https://theusajournals.com/index.php/ajast American Journal of Applied Science and Technology (ISSN: 2771-2745) sociological analysis. Moscow: IC RIOR, INFRA-M; 2012. A.V. Pogozheva. The role of dietary supplements in preventing cardiovascular diseases. Consilium Medicum 2016, Vol. 18, No. 12; pp. 55–59. Ammon N.R.T., Raul R. 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