American Journal of Research in Humanities and Social Sciences ISSN (E): 2832-8019 Volume 6, | Nov., 2022 P a g e | 38 www.americanjournal.org ESTROPHANE SOME MORPHOGENESIS OF COW BLOOD Yakubov Muzaffar Alimjonovich Samarkand State University of Veterinary Medicine, Animal Husbandry and Biotechnology Mukhtarov Elmurad Abdiglomovich Samarkand State University of Veterinary Medicine, Animal Husbandry and Biotechnology A B S T R A C T K E Y W O R D S The article describes the dynamics of changes in the shape elements of the composition of the blood of cows as a result of the pharmacokinetic and pharmacodynamic effects of the hormonal drug estrophan based on the analysis of the literature. Estrofan, hemoglobin, erythrocyte, cyanide method, lekotsid. Relevance of the Topic Our government assigns urgent tasks to veterinary science and practice, such as personal assistants, the development of effective and cost-effective methods of introducing new modern drugs used in the fight against and treatment of diseases of farm livestock, and achieving a reduction in the cost of products through their implementation. folded Hormonal drugs with various pharmacological effects have been introduced and used in animal husbandry and veterinary practice in recent months for the treatment and prevention of infertility of farm animals and various diseases of the female reproductive system. Currently, many new hormonal preparations produced abroad and produced in our domestic pharmaceutical enterprises are entering the veterinary practice of our Republic, including surfagon, estrophan, klotoprostin and others. In addition to their widespread use in practice, there is no literature information about the mechanism of action, pharmacological properties. It has been determined that sheep in different natural conditions show specific dynamics of changes in blood serum albumin in the physiological stages of postnatal ontogeny in connection with living conditions. The author's experiments proved that the blood of animals is different at different ages and living conditions, and conclusions were drawn. In the literature, there are scientific materials that have been proven in experiments that the animal's living conditions are different in the postnatal ontogenesis of the organism. It has been found that sheep in different natural conditions exhibit specific dynamics of changes in serum triglycerine in the physiological stages of postnatal ontogeny in relation to living conditions. Changes in sheep blood were mostly reversible at 6 months and 60 months of posnotol ontogenesis American Journal of Research in Humanities and Social Sciences Volume 5, Nov., 2022 P a g e | 39 www.americanjournal.org The purpose of the study. The purpose of our scientific research is to study the laws of effects of strophan drug, which affects the uterus, on blood parameters of cows. Object and methods of testing Our experiments were conducted on cows at a livestock farm specializing in breeding in Fergana district, Fergana region. The number of erythrocytes and leukocytes (on the Goryaev counting grid), hemoglobin (hemoglobin-cyanide method) of blood samples taken from cows. Experiments were carried out on 5-6 year old cows divided into 3 groups. Cows in group 1 were given 0.5 mg/kg of estrophan, cows in group 2 were given 3 mg/kg, and cows in group 3 were given 10 mg/kg intramuscularly, and the effect of the drug on the morphological indicators of cows' blood was studied. Inspection results. Before starting experiments, experimental animals were monitored for clinical signs for 16 days. All animals in the experimental group had their blood counts checked before drug administration, and at the same time, they were considered as the control group. The experimental animals were under control for 20 days. When Estrofan was administered three times at a dose of 0.5 mg/kg (the first experimental group), significant changes in the blood parameters of cows during the entire experimental period, including the number of erythrocytes (1.90 ± 2.03%), the number of leukocytes (2.60 ± 3.08%) and hemoglobin content (2.14 ± 3.76%) did not change. Estrophane at a dose of 3 mg/kg three times (experimental group two) produced significant and legitimate changes in the level of blood parameters in cows. Compared to the initial indicator, the number of erythrocytes decreased from 2.25 to 7.50% by 3, 6, 12 and 24 hours, as well as by the 3rd day of the study, that is, three days after the last administration of the drug. The greatest decrease in their number occurred in the 3rd hour of the experiment (respectively 7.51; 6.50 and 5.27%), later (from 6th day) and at the end of the experiments, compared to the initial indicators, 3, Observing that it increased by 76%. The amount of possible Hemoglobin also decreased to 2.96-10.80% during these periods of research, but it was noted that it increased to 3.93% by the last days of the experiment (day 15). The maximum decrease in the amount of hemoglobins was observed in the first 3 and 6 hours of the experiment, that is, this amount was proportionally 10.81; 7.87; 6.89; and corresponded to 5.90%. The number of leukocytes increased by 2.15-6.70% on the 1st day of the experiment, the maximum changes were observed after 6.14 and 24 hours and returned to the previous state at the end of the experiment. After 3 doses of 10 mg/kg of Estrofan (the third experimental group), the most significant changes were observed in the quantitative index of blood-forming elements. At the end of the experiment, a significant decrease in the number of erythrocytes and the amount of hemoglobin and a significant increase in the number of leukocytes were found, that is, the number of erythrocytes decreased by 14.48-20.15%, the amount of hemoglobin decreased by 16.1-22.66%, and the number of leukocytes increased It was equal to 9.25-19.31% (R 0.02). When used in the lowest and medium doses (i.e. 1-3 mg/kg), it was noted that the blood parameters of animals changed in almost the same direction, but at different levels. When using a high dose of estrophan (10 mg/kg), significant changes in blood parameters were detected, including a significant decrease in the number of erythrocytes and the quantitative percentage of hemoglobin, while the number of leukocytes, on the contrary, increased. So, the drug estrophan has a significant effect on the morphological parameters of the blood of animals at maximum doses. American Journal of Research in Humanities and Social Sciences Volume 5, Nov., 2022 P a g e | 40 www.americanjournal.org Conclusions 1. Estrofan in small doses (1 mg/kg) has almost no effect on the morphological parameters of the blood of cows (number of erythrocytes and leukocytes, amount of hemoglobin). 2. Estrofan in medium doses (3 mg/kg) partially affects the morphological parameters of the blood of cows (the number of erythrocytes and leukocytes, the amount of hemoglobin). 3. Estrofan in large doses (10 mg/kg) has a toxic-toxic effect on the morphological parameters of the blood of cows (the number of erythrocytes and leukocytes, the amount of hemoglobin). References 1. Abdig’ulomovich M. E. et al. Dynamics of triglitsrin in blood in different conditions //E Conference Zone. – 2022. – С. 202-204. 2. Abdigulomovich M. E., Bobokulovich D. N. Changes In The Postnatal Ontogenesis Of Historological Indicators Of The Four-Headed Muscle Number Of Hisori Sheep. 3. Boboqulov Avazbek, Murodullayev Javohir, & Muxtarov Elmurod. (2022). Qondagi albuminning turli shashroitlardagi ko’rsatkichlari . World Scientific Research Journal, 2(2), 128–132. Retrieved from http://wsrjournal.com/index.php/wsrj/article/view/8 4. Dilmurodov N. The Developmental Peculiarities of Tubular Bones of Autopodies of Sheep at Postnatal Ontogenesis in Dependence on Habitat Conditions //新疆农业大学学报. – 2010. – Т. 6. 5. Doniyorov Shohrukh Zafarovich, Dilmurodov Nasriddin Babakulovich. Changes In Natural And Hygroscopic Moisture Content Of Broiler Chickens In Postnatal Ontogenesis. NVEO-NATURAL VOLATILES & ESSENTIAL OILS Journal| NVEO. 2021/12/30. C 15710-15713 6. H. B. Yunusov, N.B. Dilmurodov, B.A. Kuliev, S.M. Akhmedov The Role Of Coccal Microflora In The Etiology And Pathogenesis Of Respiratory Diseases In Lambs Of The Karakul Breed Of Uzbekistan. International Journal of Advanc Science Б. 1923-1928. 7. Hakim N., Numon D., Nasriddin D. Treatment of aseptic diseases of limb distal part joints in uzbek sport horses //Journal of Microbiology, Biotechnology and Food Sciences. – 2021. – Т. 2021. – С. 478-481. 8. Mirzoev Z. R., Rakhmonov R. A., Khudoynazarova N. E. Morphometric Properties Of The Shoulder Bone In The Postnatal Ontogenesis Of Rabbits In The Meat Direction //NVEO- NATURAL VOLATILES & ESSENTIAL OILS Journal| NVEO. – 2021. – С. 15714-15717. 9. Mirzoev Z. R., Rakhmonov R. A., Khudoynazarova N. E. Morphometric Properties Of The Shoulder Bone In The Postnatal Ontogenesis Of Rabbits In The Meat Direction //NVEO- NATURAL VOLATILES & ESSENTIAL OILS Journal| NVEO. – 2021. – С. 15714-15717. 10. MUKHTAROV B. Z., DILMURODOV N. B. Some Biochemical Indicators of Blood in Prosperous Cows in Pure Pododermatitis //JournalNX. – Т. 6. – №. 06. – С. 58-62. 11. Niyozov H. B. Etiology, incidence rate and clinical symptoms of postpateral endometrits of pedigree cows //Financed by the Erasmus+ programme of the European Union The conclusions and view expressed herein are those of the authors and do not necessarily reflect an official view of the European Commission. – 2020. 12. Niyozov H. B. Etopathogenesis, incidence rate and clinical symptoms of purulent-necrotic processes of cattle fingers //Financed by the Erasmus+ programme of the European Union The American Journal of Research in Humanities and Social Sciences Volume 5, Nov., 2022 P a g e | 41 www.americanjournal.org conclusions and view expressed herein are those of the authors and do not necessarily reflect an official view of the European Commission. – 2020. 13. Normuradova Z. F. et al. QUYONLARNING BIOLOGIK XUSUSIYATLARI //E Conference Zone. – 2022. – С. 44-47. 14. Normuradova, Z. F., & Arzikulova, S. M. (2022, May). Quyonlarning biologik xususiyatlari. In E Conference Zone (pp. 44-47). 15. Oybek A., Elmurod M. Morphometric changes of skeletal muscles of animals in the postnatal period (review of literature) //Conferencea. – 2022. – С. 161-165. 16. Shuxratovna R. G., Babakulovich D. N., Nikolayevich F. D. Anatomical Structure of Reproductive Organs of Chickens in the Egg Direction //Middle European Scientific Bulletin. – 2022. – Т. 24. – С. 240-243. 17. Shuxratovna R. G., Babakulovich D. N., Nikolayevich F. D. Anatomical Structure of Reproductive Organs of Chickens in the Egg Direction //Middle European Scientific Bulletin. – 2022. – Т. 24. – С. 240-243. 18. Ulomovich m. E. A., Babakulovich D. N. Morphogenesis of the hind leg distal muscles of hissar sheep of different breeds in different ecological conditions. 19. Yaxshiyeva S. X. et al. Ross-308 krossiga mansub broyler jo ‘jalar muskulli oshqozonning postnatal ontogenezi //Gospodarka i Innowacje. – 2022. – Т. 24. – С. 926-930. 20. Zafarovich D. S., Babakulovich D. N. Changes In Natural And Hygroscopic Moisture Content Of Broiler Chickens In Postnatal Ontogenesis //NVEO-NATURAL VOLATILES & ESSENTIAL OILS Journal| NVEO. – 2021. – С. 15710-15713. 21. Zafarovich D. S., Babakulovich D. N., Norboyevich C. O. Changes in the Amount of Calcium and Phosphorus in the Composition of the Femur Bone of Broiler Chickens in Postnatal Ontogenesis //International Journal of Innovative Analyses and Emerging Technology. – 2022. – Т. 2. – №. 2. – С. 21-25. 22. Zarpullayev P., Dilmurodov N. Ferula assafoetida o ‘simligining hayvonlarning reproduktiv faoliyatiga TA’SIRI //Conferencea. – 2022. – С. 88-90. 23. ДИЛМУРОДОВ Н. Б. Физические параметры метаподия овец гиссарской породы в постнатальном онтогенезе //Вестник ветеринарии. – 2015. – №. 4. – С. 58-60. 24. Дилмуродов Н. Б., Дониёров Ш. З., Султонов Б. А. Бройлер жўжалари узангилик (цевка) суягининг морфогенезига пробиотиклар таъсири //Вестник Ветеринарии и Животноводства. – 2021. – Т. 1. – №. 2. 25. Дилмуродов Н. Б., Дониёров Ш. З., Чориев О. Н. Бройлер жўжалар елка суяги таркибидаги кул ва умумий органик моддалар миқдорини постнатал онтогенезда ўзгариши //Вестник Ветеринарии и Животноводства. – 2022. – Т. 2. – №. 1. 26. Дилмуродов Н., Мухторов Э. Турли яшаш шароитидаги ҳисори зотли қўйлар постнатал онтогенезида оёқлар проксимал мускулларининг морфометрик хусусиятлари //Вестник Ветеринарии и Животноводства. – 2021. – Т. 1. – №. 1. 27. Кулиев Б.А., Ахмедов С.М., Зайниддинов Б.Х. Лечение т-активином ягнят каракульской породы, больных пневмонией. Витебск ВГАВМ 2019, Б. 123-125. 28. Кулиев Б.А., Ахмедов С.М., Зайниддинов Б.Х. Лечение т-активином ягнят каракульской породы, больных пневмонией. Витебск ВГАВМ 2019, Б. 123-125 American Journal of Research in Humanities and Social Sciences Volume 5, Nov., 2022 P a g e | 42 www.americanjournal.org 29. Кулиева Б. А., Акрамов К. Ш. Патоморфология пневмоний у ягнят каракульской породы. – 2021. 30. Мухторов Э. А. Действие условия содержания на морфологические показатели мускулатуры конечности постнатального онтогенеза у гиссарской породы овец //современное состояние, традиции и инновационные технологии в развитии апк. – 2020. – с. 137-140. 31. Мухторов Э. А. Ҳисори зотли қўйлар орқа оёқ мускулларининг постнатал онтогенездаги морфометрик хусусиятлари //журнал агро процессинг. – 2019. – №. 4. 32. Мухторов Э. А., Дилмуродов Н. Б. Ҳисори зотли қўйлар постнатал онтогенезида оёқ мускулларининг морфологик кўрсаткичларига яшаш шароитини таъсири //журнал агро процессинг. – 2020. – т. 2. – №. 2. 33. Мухторов Э., Дилмуродов Н. Ҳисори зотли қўйлар елканинг сонниг тўрт бошли мускули толасининг ядроси диаметрини постнатал онтогенезда ўзгариши //International Conference on Agriculture Sciences, Environment, Urban and Rural Development. – 2021. – С. 49-52. 34. Насриддин Бабакулович Дилмуродов, Шохрух Зафарович Дониёров, Отабек Норбоевич Чориев. Бройлер жўжалар елка суяги таркибидаги кул ва умумий органик моддалар миқдорини постнатал онтогенезда ўзгариши. Вестник Ветеринарии и Животноводства. 2022/2/2 35. Рахманова Г. Ш., Федотов Д. Н. особенности гистологического строения яичника у кур- молодок //научное обеспечение животноводства сибири. – 2021. – с. 466-467. 36. Раҳмонов, Ў. А., Сапаров, А. Р., & Азимова, Д. М. (2022). Катарал кератоконъюнктивитларни даволашда ноанъанавий усулларни қўллаш. Eurasian journal of medical and natural sciences, 2(6), 401-404. 37. Раҳмонов, Ў. А., Сапаров, А. Р., & Қахарова, М. К. (2022). Отларда йирингли коньюнктивитни даволаш. Eurasian journal of medical and natural sciences, 2(6), 405-408. 38. Раҳмонов, Ў., Сапаров, А., & Азимова, Д. (2022). Катарал кератоконъюнктивитларни даволашда ноанъанавий усулларни қўллаш. Eurasian journal of medical and natural sciences, 2(6), 401–404. 39. Раҳмонов, Ў., Сапаров, А., & Қахарова, М. (2022). Отларда йирингли коньюнктивитни даволаш. Eurasian journal of medical and natural sciences, 2(6), 405–408. 40. Таштемиров Р. М. и др. Teri kasalliklarini davolashda qo ‘llanadigan o ‘simlik dori vositalarining tavsifi (Adabiyot malumotlari asosida) //Вестник Ветеринарии и Животноводства. – 2022. – Т. 2. – №. 1.