



































International Journal of Biological Engineering and Agriculture


American Journal of Science and  

Learning for Development 
 

Volume 1 | No 2 | Dec-2022 

 

 

 
Published by inter-publishing.com  |  All rights reserved. © 2022 
Journal Homepage: https://inter-publishing.com/index.php/IJISE    

Page 177 

 

 

 
Chromoto-Mass Methods for Detecting Simple Esters in 

Chromatography-Mass Spectrometry Method 

 
 

Kholmirzaev Mekhroj Murodillayevich 
1
, Khaydarov Gayrat Shoyimovich 

2
,  

 

Saitkulov Foziljon Ergashevich 
3
, Kholiqova Kamola O’tkir qizi 

4
, Umarova Aziza Ikrom qizi 

5 

 

 
1, 2 

Uzbekistan-Finland Pedagogical Institute, Faculty of Natural and Physical Culture, Department 

of Natural Sciences 

3 
Tashkent State Agrarian University 

4, 5 
Student Tashkent State Agrarian University 

  
  
 

Abstract: This paper shows a detailed study of the features of the structural organization of 

compounds that determine the chemical composition of the organic substance cuminium, on the 

example of its ethanol extract, using chromatography-mass spectrometry, expanding the set of 

individual compounds, in addition to those known in the scientific literature, to calculate the 

composition of the extract of esters. 
 
Keywords: Detailed study of the structural organization of compounds, ethanol extract, 

chromatography-mass spectrometry, individual compounds, mannan, hydrolyzed to mannose, as well 

as dextrins, starch, protein substances, pentosans, methylpentosans, sugars, esters simple and 

complex, mineral salts. 

 
  
Introduction 

A detailed study of the features of the structural organization of compounds that determine the 

chemical composition of the organic substance cuminium, on the example of its ethanol extract, 

using chromatography-mass spectrometry, expanding the set of individual compounds, in addition to 

those known in the scientific literature, calculate the structural and group composition of the extract, 

in order to determine the quantitative ratio of various groups of components, to obtain their mass-

spectra and structural formulas; to make an assumption about the direction of the pharmacological 

action of cuminium preparations, taking into account the peculiarities of the structure of compounds 

of its organic matter. 

Medicinal raw materials were cuminium seeds collected by cuminium. In dried form, they are called 

salep. The chemical composition of cuminium is determined by the content of mucus, the basis of 

which is determined by the high-molecular polysaccharide mannan, hydrolyzed to mannose, as well 

as dextrins, starch, protein substances, bitterness, pentosans, methylpentosans, sugars, esters simple 

and complex, mineral salts and others [1-10]. 

Cumin is used as a seasoning in cooking. Sometimes it is used instead of pepper. Cumin is suitable 

for any dish. This is another advantage of it compared to other seasonings. It is also used in medicine 

to improve the condition of diabetes, in the treatment of anemia and pathologies of the nervous 

system. regulates carbohydrate, water-salt metabolism, the functional state of the male sex glands [1-

10]. 



 

American Journal of Science and  Learning for Development Volume 1, No 2|    
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Methods and Results 

The object of the study was an ethanol extract of cuminium obtained by exhaustive extraction of raw 

materials with ethanol with a mass of 95% in a Scraper apparatus. The extract was released from 

ethanol in a vacuum rotary evaporator model RE-52 AA Rotary Evaporator, the residue was weighed 

and its chemical composition was studied by chromatography-mass spectrometry. 

The registration of analytical signals was carried out with the following parameters of the mass 

spectrometer:the temperature of the transition line and the ion source is 250 and 280°C, respectively, 

electronic ionization (EI), the range of recorded masses is from 50 to 500 Da. 

The composition of esters is dominated by simple esters of the type: But-1-ene-3-yne-1-ethoxy, 

Cyclohexane, ethoxy, 2,7-Octadiene, 1-butoxy. 

 

Fig 1. 

 

Fig 2. 



 

American Journal of Science and  Learning for Development Volume 1, No 2|    
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Fig 3. 

It is shown (Fig. 1-3) that cumin contains esters in the spectrum. 

The given list of compounds, the features of their structural organization, the presence of various 

functional groups in them allows us to conclude about the rather complex chemical composition of 

the organic matter of the spotted hawthorn, and, consequently, about the specificity of its 

pharmacological action. 

Conclusion 

Cumin’s found in the extract are responsible for antispasmodic, anticoagulant, photosensitizing, 

antitumor effects, especially in combination with steroid compounds, aldehydes and ketones. 

Nitrogen- and sulfur-containing compounds have a highly selective pharmacological effect, 

significant amounts of which, different in structure, are found in cumin extract. 

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American Journal of Science and  Learning for Development Volume 1, No 2|    
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Journal Homepage: http://inter-publishing.com/index.php/AJSLD    

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