id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
american_scientific_journal-2511	Al-Badran, Adnan; Al-Fadal, Sabaa	Cloning, Expression of Hirudin Gene from Leech (Hirudo orientalis) in BL21(DE3) Strain	2017	18	.pdf	application/pdf	6584	298	49	Leech type was detected as H. orientalis by using two types of genes 18SrDNA and CO-I genes, Hirudin gene was amplified by specific primers from H. orientalis cDNA. Furthermore, Hirudin gene was expressed in BL21(DE3) strain under the control of T7 promoter in pET-16b vector, constructed vector pET-16-HR vector was extracted and used to amplify hirudin gene by specific primers, then hirudin gene band was appeared after digestion of extracted plasmid with Hind III and NdeI restriction enzyme.	cache/american_scientific_journal-2511.pdf	txt/american_scientific_journal-2511.txt