id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
american_scientific_journal-4355	Zakzouk, Ahmed A.A.; Osman, Mohamed S.A.; Zeaneddin, Ramadan A; Khalifa, Hamdeen A	Solving the Multi-objective Facility Location Problem Using Big Bang Big Crunch and Pigeon Inspired Optimization Techniques	2018	28	.docx	application/vnd.openxmlformats-officedocument.wordprocessingml.document	9860	580	57	It has already been demonstrated that proximal tubular epithelial cells (PTC) have the potential to contribute to the pathogenesis of renal fibrosis directly by alterations in the production of components of the extracellular matrix and indirectly by the production of profibrotic cytokines [35;36], and we can see betaglycan expression by Cytokines and TGF-β by different methods,( figures 4,5) : Figure 5: Betaglycan Expression by Conventional PCR 2.7. Conventional PCR showing betaglycan expression as bands on agarose gel following No stimulation, following IL-1 and following IL-6 stimulation compared with actin bands Figure 6: Conventional PCR Confirmation by Quantitative (Q-PCR), Concentration dependent induction (IL-1, IL-6 and TGF-β) of betaglycan expression To identify the most effective dose of IL-1, IL-6 or TGF-β that produced the greatest betaglycan expression, different concentrations of cytokines were used to stimulate HK2 cells at the optimal time which was chosen as 12 hours.	cache/american_scientific_journal-4355.docx	txt/american_scientific_journal-4355.txt