id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
american_scientific_journal-4495	Al-helfawi, Mohamed Ali; Phiillips, Aled; Steadman, Robert	Betaglycan as a Potential Regulator of TGF-?1 Signalling in the Kidney	2018	28	.pdf	application/pdf	10174	609	57	Conventional PCR showing betaglycan expression as bands on agarose gel following No stimulation, following IL-1 and following IL-6 stimulation compared with actin bands betaglycan ≈300 bp with no stimulus 123 b p DNA Ladder 10M 3H 6H 12H 24H C 3 H 6 H 12H 24H C 3H 6 H 12H 24H betaglycan ≈300 bp IL-1 IL-6 beta-Actin Bands ≈204 bp 123 b p DNA Ladder 3 H 6 H 12H 24H C betaglycan ≈300 bp IL-1 3H 6 H 12H 24H C IL-6 3 IL-1 IL-6 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2018) Volume 50, No 1, pp 22-49 31 Figure 6: Conventional PCR Confirmation by Quantitative (Q-PCR), Concentration dependent induction (IL-1, IL-6 and TGF-β) of betaglycan expression To identify the most effective dose of IL-1, IL-6 or TGF-β that produced the greatest betaglycan expression, different concentrations of cytokines were used to stimulate HK2 cells at the optimal time which was chosen as 12 hours. Figure 17: Demonstration of the HK2 cell functions in terms of betaglycan expression after being knocked- down then stimulated by TGF-β: American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2018) Volume 50, No 1, pp 22-49 39 This Q-PCR bar graph illustrates betaglycan Expression after betaglycan knock-down then TGF-β stimulation at different time points, every sample compared to the scramble.	cache/american_scientific_journal-4495.pdf	txt/american_scientific_journal-4495.txt