id	sid	tid	token	lemma	pos
american_scientific_journal-2511	1	1	25	25	NUM
american_scientific_journal-2511	1	2	american	american	ADJ
american_scientific_journal-2511	1	3	scientific	scientific	ADJ
american_scientific_journal-2511	1	4	research	research	NOUN
american_scientific_journal-2511	1	5	journal	journal	NOUN
american_scientific_journal-2511	1	6	for	for	ADP
american_scientific_journal-2511	1	7	engineering	engineering	NOUN
american_scientific_journal-2511	1	8	,	,	PUNCT
american_scientific_journal-2511	1	9	technology	technology	NOUN
american_scientific_journal-2511	1	10	,	,	PUNCT
american_scientific_journal-2511	1	11	and	and	CCONJ
american_scientific_journal-2511	1	12	sciences	science	NOUN
american_scientific_journal-2511	1	13	(	(	PUNCT
american_scientific_journal-2511	1	14	asrjets	asrjet	NOUN
american_scientific_journal-2511	1	15	)	)	PUNCT
american_scientific_journal-2511	1	16	issn	issn	PROPN
american_scientific_journal-2511	1	17	(	(	PUNCT
american_scientific_journal-2511	1	18	print	print	NOUN
american_scientific_journal-2511	1	19	)	)	PUNCT
american_scientific_journal-2511	1	20	2313	2313	NUM
american_scientific_journal-2511	1	21	-	-	SYM
american_scientific_journal-2511	1	22	4410	4410	NUM
american_scientific_journal-2511	1	23	,	,	PUNCT
american_scientific_journal-2511	1	24	issn	issn	PROPN
american_scientific_journal-2511	1	25	(	(	PUNCT
american_scientific_journal-2511	1	26	online	online	ADJ
american_scientific_journal-2511	1	27	)	)	PUNCT
american_scientific_journal-2511	1	28	2313	2313	NUM
american_scientific_journal-2511	1	29	-	-	SYM
american_scientific_journal-2511	1	30	4402	4402	NUM
american_scientific_journal-2511	1	31	©	©	PROPN
american_scientific_journal-2511	1	32	global	global	ADJ
american_scientific_journal-2511	1	33	society	society	NOUN
american_scientific_journal-2511	1	34	of	of	ADP
american_scientific_journal-2511	1	35	scientific	scientific	ADJ
american_scientific_journal-2511	1	36	research	research	NOUN
american_scientific_journal-2511	1	37	and	and	CCONJ
american_scientific_journal-2511	1	38	researchers	researcher	NOUN
american_scientific_journal-2511	1	39	http://asrjetsjournal.org/	http://asrjetsjournal.org/	NOUN
american_scientific_journal-2511	1	40	cloning	cloning	NOUN
american_scientific_journal-2511	1	41	,	,	PUNCT
american_scientific_journal-2511	1	42	expression	expression	NOUN
american_scientific_journal-2511	1	43	of	of	ADP
american_scientific_journal-2511	1	44	hirudin	hirudin	VERB
american_scientific_journal-2511	1	45	gene	gene	NOUN
american_scientific_journal-2511	1	46	from	from	ADP
american_scientific_journal-2511	1	47	leech	leech	NOUN
american_scientific_journal-2511	1	48	(	(	PUNCT
american_scientific_journal-2511	1	49	hirudo	hirudo	NOUN
american_scientific_journal-2511	1	50	orientalis	orientalis	NOUN
american_scientific_journal-2511	1	51	)	)	PUNCT
american_scientific_journal-2511	1	52	in	in	ADP
american_scientific_journal-2511	1	53	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2511	1	54	)	)	PUNCT
american_scientific_journal-2511	1	55	strain	strain	NOUN
american_scientific_journal-2511	1	56	adnan	adnan	PROPN
american_scientific_journal-2511	1	57	al	al	PROPN
american_scientific_journal-2511	1	58	-	-	PUNCT
american_scientific_journal-2511	1	59	badrana	badrana	PROPN
american_scientific_journal-2511	1	60	,	,	PUNCT
american_scientific_journal-2511	1	61	sabaa	sabaa	PROPN
american_scientific_journal-2511	1	62	al	al	PROPN
american_scientific_journal-2511	1	63	-	-	PUNCT
american_scientific_journal-2511	1	64	fadalb	fadalb	PROPN
american_scientific_journal-2511	1	65	*	*	PUNCT
american_scientific_journal-2511	1	66	auniversity	auniversity	NOUN
american_scientific_journal-2511	1	67	of	of	ADP
american_scientific_journal-2511	1	68	basrah	basrah	PROPN
american_scientific_journal-2511	1	69	,	,	PUNCT
american_scientific_journal-2511	1	70	college	college	NOUN
american_scientific_journal-2511	1	71	of	of	ADP
american_scientific_journal-2511	1	72	science	science	NOUN
american_scientific_journal-2511	1	73	,	,	PUNCT
american_scientific_journal-2511	1	74	biology	biology	NOUN
american_scientific_journal-2511	1	75	department	department	NOUN
american_scientific_journal-2511	1	76	,	,	PUNCT
american_scientific_journal-2511	1	77	basra	basra	PROPN
american_scientific_journal-2511	1	78	,	,	PUNCT
american_scientific_journal-2511	1	79	iraq	iraq	PROPN
american_scientific_journal-2511	1	80	buniversity	buniversity	NOUN
american_scientific_journal-2511	1	81	of	of	ADP
american_scientific_journal-2511	1	82	basrah	basrah	PROPN
american_scientific_journal-2511	1	83	,	,	PUNCT
american_scientific_journal-2511	1	84	college	college	NOUN
american_scientific_journal-2511	1	85	of	of	ADP
american_scientific_journal-2511	1	86	pharmacy	pharmacy	NOUN
american_scientific_journal-2511	1	87	,	,	PUNCT
american_scientific_journal-2511	1	88	pharmacognosay	pharmacognosay	PROPN
american_scientific_journal-2511	1	89	department	department	PROPN
american_scientific_journal-2511	1	90	,	,	PUNCT
american_scientific_journal-2511	1	91	basra	basra	PROPN
american_scientific_journal-2511	1	92	,	,	PUNCT
american_scientific_journal-2511	1	93	iraq	iraq	PROPN
american_scientific_journal-2511	1	94	aemail	aemail	NOUN
american_scientific_journal-2511	1	95	:	:	PUNCT
american_scientific_journal-2511	1	96	aalbadran@yahoo.com	aalbadran@yahoo.com	X
american_scientific_journal-2511	1	97	bemail	bemail	NOUN
american_scientific_journal-2511	1	98	:	:	PUNCT
american_scientific_journal-2511	1	99	sabaaal.fadal@gmail.com	sabaaal.fadal@gmail.com	X
american_scientific_journal-2511	1	100	abstract	abstract	NOUN
american_scientific_journal-2511	1	101	the	the	DET
american_scientific_journal-2511	1	102	use	use	NOUN
american_scientific_journal-2511	1	103	of	of	ADP
american_scientific_journal-2511	1	104	leeches	leech	NOUN
american_scientific_journal-2511	1	105	in	in	ADP
american_scientific_journal-2511	1	106	bloodletting	bloodlette	VERB
american_scientific_journal-2511	1	107	therapy	therapy	NOUN
american_scientific_journal-2511	1	108	is	be	AUX
american_scientific_journal-2511	1	109	one	one	NUM
american_scientific_journal-2511	1	110	of	of	ADP
american_scientific_journal-2511	1	111	the	the	DET
american_scientific_journal-2511	1	112	most	most	ADV
american_scientific_journal-2511	1	113	important	important	ADJ
american_scientific_journal-2511	1	114	techniques	technique	NOUN
american_scientific_journal-2511	1	115	in	in	ADP
american_scientific_journal-2511	1	116	ancient	ancient	ADJ
american_scientific_journal-2511	1	117	medicine	medicine	NOUN
american_scientific_journal-2511	1	118	from	from	ADP
american_scientific_journal-2511	1	119	greek	greek	ADJ
american_scientific_journal-2511	1	120	time	time	NOUN
american_scientific_journal-2511	1	121	up	up	ADP
american_scientific_journal-2511	1	122	to	to	ADP
american_scientific_journal-2511	1	123	now	now	ADV
american_scientific_journal-2511	1	124	.	.	PUNCT
american_scientific_journal-2511	2	1	leeches	leech	NOUN
american_scientific_journal-2511	2	2	saliva	saliva	NOUN
american_scientific_journal-2511	2	3	contain	contain	VERB
american_scientific_journal-2511	2	4	anticoagulant	anticoagulant	ADJ
american_scientific_journal-2511	2	5	hirudin	hirudin	NOUN
american_scientific_journal-2511	2	6	which	which	PRON
american_scientific_journal-2511	2	7	facilitate	facilitate	VERB
american_scientific_journal-2511	2	8	blood	blood	NOUN
american_scientific_journal-2511	2	9	flow	flow	NOUN
american_scientific_journal-2511	2	10	,	,	PUNCT
american_scientific_journal-2511	2	11	this	this	PRON
american_scientific_journal-2511	2	12	is	be	AUX
american_scientific_journal-2511	2	13	one	one	NUM
american_scientific_journal-2511	2	14	reason	reason	NOUN
american_scientific_journal-2511	2	15	for	for	ADP
american_scientific_journal-2511	2	16	using	use	VERB
american_scientific_journal-2511	2	17	leeches	leech	NOUN
american_scientific_journal-2511	2	18	to	to	PART
american_scientific_journal-2511	2	19	save	save	VERB
american_scientific_journal-2511	2	20	thousands	thousand	NOUN
american_scientific_journal-2511	2	21	of	of	ADP
american_scientific_journal-2511	2	22	severed	severed	ADJ
american_scientific_journal-2511	2	23	fingers	finger	NOUN
american_scientific_journal-2511	2	24	,	,	PUNCT
american_scientific_journal-2511	2	25	noses	nose	NOUN
american_scientific_journal-2511	2	26	and	and	CCONJ
american_scientific_journal-2511	2	27	ears	ear	NOUN
american_scientific_journal-2511	2	28	in	in	ADP
american_scientific_journal-2511	2	29	recent	recent	ADJ
american_scientific_journal-2511	2	30	years	year	NOUN
american_scientific_journal-2511	2	31	.	.	PUNCT
american_scientific_journal-2511	3	1	in	in	ADP
american_scientific_journal-2511	3	2	this	this	DET
american_scientific_journal-2511	3	3	study	study	NOUN
american_scientific_journal-2511	3	4	,	,	PUNCT
american_scientific_journal-2511	3	5	hirudin	hirudin	VERB
american_scientific_journal-2511	3	6	gene	gene	NOUN
american_scientific_journal-2511	3	7	successfully	successfully	ADV
american_scientific_journal-2511	3	8	cloning	clone	VERB
american_scientific_journal-2511	3	9	and	and	CCONJ
american_scientific_journal-2511	3	10	expressed	express	VERB
american_scientific_journal-2511	3	11	in	in	ADP
american_scientific_journal-2511	3	12	bl21(de3	bl21(de3	VERB
american_scientific_journal-2511	3	13	)	)	PUNCT
american_scientific_journal-2511	3	14	strain	strain	NOUN
american_scientific_journal-2511	3	15	.	.	PUNCT
american_scientific_journal-2511	4	1	leech	leech	NOUN
american_scientific_journal-2511	4	2	type	type	NOUN
american_scientific_journal-2511	4	3	was	be	AUX
american_scientific_journal-2511	4	4	detected	detect	VERB
american_scientific_journal-2511	4	5	as	as	ADP
american_scientific_journal-2511	4	6	h.	h.	NOUN
american_scientific_journal-2511	4	7	orientalis	orientali	NOUN
american_scientific_journal-2511	4	8	by	by	ADP
american_scientific_journal-2511	4	9	using	use	VERB
american_scientific_journal-2511	4	10	two	two	NUM
american_scientific_journal-2511	4	11	types	type	NOUN
american_scientific_journal-2511	4	12	of	of	ADP
american_scientific_journal-2511	4	13	genes	gene	NOUN
american_scientific_journal-2511	4	14	18srdna	18srdna	PUNCT
american_scientific_journal-2511	4	15	and	and	CCONJ
american_scientific_journal-2511	4	16	co	co	NOUN
american_scientific_journal-2511	4	17	-	-	NOUN
american_scientific_journal-2511	4	18	i	i	PRON
american_scientific_journal-2511	4	19	genes	gene	NOUN
american_scientific_journal-2511	4	20	,	,	PUNCT
american_scientific_journal-2511	4	21	hirudin	hirudin	VERB
american_scientific_journal-2511	4	22	gene	gene	NOUN
american_scientific_journal-2511	4	23	was	be	AUX
american_scientific_journal-2511	4	24	amplified	amplify	VERB
american_scientific_journal-2511	4	25	by	by	ADP
american_scientific_journal-2511	4	26	specific	specific	ADJ
american_scientific_journal-2511	4	27	primers	primer	NOUN
american_scientific_journal-2511	4	28	from	from	ADP
american_scientific_journal-2511	4	29	h.	h.	PROPN
american_scientific_journal-2511	4	30	orientalis	orientalis	PROPN
american_scientific_journal-2511	4	31	cdna	cdna	PROPN
american_scientific_journal-2511	4	32	.	.	PUNCT
american_scientific_journal-2511	5	1	furthermore	furthermore	ADV
american_scientific_journal-2511	5	2	,	,	PUNCT
american_scientific_journal-2511	5	3	hirudin	hirudin	VERB
american_scientific_journal-2511	5	4	gene	gene	NOUN
american_scientific_journal-2511	5	5	was	be	AUX
american_scientific_journal-2511	5	6	expressed	express	VERB
american_scientific_journal-2511	5	7	in	in	ADP
american_scientific_journal-2511	5	8	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2511	5	9	)	)	PUNCT
american_scientific_journal-2511	5	10	strain	strain	NOUN
american_scientific_journal-2511	5	11	under	under	ADP
american_scientific_journal-2511	5	12	the	the	DET
american_scientific_journal-2511	5	13	control	control	NOUN
american_scientific_journal-2511	5	14	of	of	ADP
american_scientific_journal-2511	5	15	t7	t7	PROPN
american_scientific_journal-2511	5	16	promoter	promoter	NOUN
american_scientific_journal-2511	5	17	in	in	ADP
american_scientific_journal-2511	5	18	pet-16b	pet-16b	NUM
american_scientific_journal-2511	5	19	vector	vector	NOUN
american_scientific_journal-2511	5	20	,	,	PUNCT
american_scientific_journal-2511	5	21	constructed	construct	VERB
american_scientific_journal-2511	5	22	vector	vector	NOUN
american_scientific_journal-2511	5	23	pet-16	pet-16	PROPN
american_scientific_journal-2511	5	24	-	-	ADJ
american_scientific_journal-2511	5	25	hr	hr	NOUN
american_scientific_journal-2511	5	26	vector	vector	NOUN
american_scientific_journal-2511	5	27	was	be	AUX
american_scientific_journal-2511	5	28	extracted	extract	VERB
american_scientific_journal-2511	5	29	and	and	CCONJ
american_scientific_journal-2511	5	30	used	use	VERB
american_scientific_journal-2511	5	31	to	to	PART
american_scientific_journal-2511	5	32	amplify	amplify	VERB
american_scientific_journal-2511	5	33	hirudin	hirudin	VERB
american_scientific_journal-2511	5	34	gene	gene	NOUN
american_scientific_journal-2511	5	35	by	by	ADP
american_scientific_journal-2511	5	36	specific	specific	ADJ
american_scientific_journal-2511	5	37	primers	primer	NOUN
american_scientific_journal-2511	5	38	,	,	PUNCT
american_scientific_journal-2511	5	39	then	then	ADV
american_scientific_journal-2511	5	40	hirudin	hirudin	VERB
american_scientific_journal-2511	5	41	gene	gene	NOUN
american_scientific_journal-2511	5	42	band	band	NOUN
american_scientific_journal-2511	5	43	was	be	AUX
american_scientific_journal-2511	5	44	appeared	appear	VERB
american_scientific_journal-2511	5	45	after	after	ADP
american_scientific_journal-2511	5	46	digestion	digestion	NOUN
american_scientific_journal-2511	5	47	of	of	ADP
american_scientific_journal-2511	5	48	extracted	extract	VERB
american_scientific_journal-2511	5	49	plasmid	plasmid	NOUN
american_scientific_journal-2511	5	50	with	with	ADP
american_scientific_journal-2511	5	51	hind	hind	NOUN
american_scientific_journal-2511	5	52	iii	iii	NOUN
american_scientific_journal-2511	5	53	and	and	CCONJ
american_scientific_journal-2511	5	54	ndei	ndei	NOUN
american_scientific_journal-2511	5	55	restriction	restriction	NOUN
american_scientific_journal-2511	5	56	enzyme	enzyme	NOUN
american_scientific_journal-2511	5	57	.	.	PUNCT
american_scientific_journal-2511	6	1	hirudin	hirudin	VERB
american_scientific_journal-2511	6	2	expression	expression	NOUN
american_scientific_journal-2511	6	3	was	be	AUX
american_scientific_journal-2511	6	4	established	establish	VERB
american_scientific_journal-2511	6	5	by	by	ADP
american_scientific_journal-2511	6	6	real	real	ADJ
american_scientific_journal-2511	6	7	-	-	PUNCT
american_scientific_journal-2511	6	8	time	time	NOUN
american_scientific_journal-2511	6	9	pcr	pcr	NOUN
american_scientific_journal-2511	6	10	.	.	PUNCT
american_scientific_journal-2511	7	1	production	production	NOUN
american_scientific_journal-2511	7	2	of	of	ADP
american_scientific_journal-2511	7	3	hirudin	hirudin	VERB
american_scientific_journal-2511	7	4	established	establish	VERB
american_scientific_journal-2511	7	5	in	in	ADP
american_scientific_journal-2511	7	6	lb	lb	NUM
american_scientific_journal-2511	7	7	medium	medium	NOUN
american_scientific_journal-2511	7	8	and	and	CCONJ
american_scientific_journal-2511	7	9	purified	purify	VERB
american_scientific_journal-2511	7	10	by	by	ADP
american_scientific_journal-2511	7	11	imac	imac	NOUN
american_scientific_journal-2511	7	12	column	column	NOUN
american_scientific_journal-2511	7	13	,	,	PUNCT
american_scientific_journal-2511	7	14	deae	deae	PROPN
american_scientific_journal-2511	7	15	sepharose	sepharose	NOUN
american_scientific_journal-2511	7	16	and	and	CCONJ
american_scientific_journal-2511	7	17	sp	sp	ADP
american_scientific_journal-2511	7	18	sepharose	sepharose	NOUN
american_scientific_journal-2511	7	19	.	.	PUNCT
american_scientific_journal-2511	8	1	concentration	concentration	NOUN
american_scientific_journal-2511	8	2	of	of	ADP
american_scientific_journal-2511	8	3	produced	produce	VERB
american_scientific_journal-2511	8	4	hirudin	hirudin	NOUN
american_scientific_journal-2511	8	5	within	within	ADP
american_scientific_journal-2511	8	6	its	its	PRON
american_scientific_journal-2511	8	7	solution	solution	NOUN
american_scientific_journal-2511	8	8	was	be	AUX
american_scientific_journal-2511	8	9	measured	measure	VERB
american_scientific_journal-2511	8	10	by	by	ADP
american_scientific_journal-2511	8	11	elisa	elisa	PROPN
american_scientific_journal-2511	8	12	kit	kit	NOUN
american_scientific_journal-2511	8	13	which	which	PRON
american_scientific_journal-2511	8	14	reached	reach	VERB
american_scientific_journal-2511	8	15	to	to	ADP
american_scientific_journal-2511	8	16	1.35ng	1.35ng	NUM
american_scientific_journal-2511	8	17	,	,	PUNCT
american_scientific_journal-2511	8	18	thrombin	thrombin	NOUN
american_scientific_journal-2511	8	19	titration	titration	NOUN
american_scientific_journal-2511	8	20	method	method	NOUN
american_scientific_journal-2511	8	21	was	be	AUX
american_scientific_journal-2511	8	22	used	use	VERB
american_scientific_journal-2511	8	23	to	to	PART
american_scientific_journal-2511	8	24	determine	determine	VERB
american_scientific_journal-2511	8	25	hirudin	hirudin	VERB
american_scientific_journal-2511	8	26	activity	activity	NOUN
american_scientific_journal-2511	8	27	which	which	PRON
american_scientific_journal-2511	8	28	showed	show	VERB
american_scientific_journal-2511	8	29	hirudin	hirudin	VERB
american_scientific_journal-2511	8	30	protein	protein	NOUN
american_scientific_journal-2511	8	31	required	require	VERB
american_scientific_journal-2511	8	32	360µl	360µl	NOUN
american_scientific_journal-2511	8	33	from	from	ADP
american_scientific_journal-2511	8	34	thrombin	thrombin	NOUN
american_scientific_journal-2511	8	35	for	for	ADP
american_scientific_journal-2511	8	36	clot	clot	NOUN
american_scientific_journal-2511	8	37	formation	formation	NOUN
american_scientific_journal-2511	8	38	.	.	PUNCT
american_scientific_journal-2511	9	1	keyword	keyword	NOUN
american_scientific_journal-2511	9	2	:	:	PUNCT
american_scientific_journal-2511	9	3	hirudin	hirudin	NOUN
american_scientific_journal-2511	9	4	;	;	PUNCT
american_scientific_journal-2511	9	5	hirudo	hirudo	NOUN
american_scientific_journal-2511	9	6	orientalis	orientali	NOUN
american_scientific_journal-2511	9	7	;	;	PUNCT
american_scientific_journal-2511	9	8	real	real	ADJ
american_scientific_journal-2511	9	9	-	-	PUNCT
american_scientific_journal-2511	9	10	time	time	NOUN
american_scientific_journal-2511	9	11	pcr	pcr	NOUN
american_scientific_journal-2511	9	12	;	;	PUNCT
american_scientific_journal-2511	9	13	deae	deae	NOUN
american_scientific_journal-2511	9	14	sepharose	sepharose	NOUN
american_scientific_journal-2511	9	15	;	;	PUNCT
american_scientific_journal-2511	9	16	sp	sp	ADP
american_scientific_journal-2511	9	17	sepharose	sepharose	NOUN
american_scientific_journal-2511	9	18	.	.	PUNCT
american_scientific_journal-2511	10	1	-----------------------------------------------------------------------	-----------------------------------------------------------------------	PUNCT
american_scientific_journal-2511	11	1	*	*	PUNCT
american_scientific_journal-2511	11	2	corresponding	correspond	VERB
american_scientific_journal-2511	11	3	author	author	NOUN
american_scientific_journal-2511	11	4	.	.	PUNCT
american_scientific_journal-2511	12	1	http://asrjetsjournal.org/	http://asrjetsjournal.org/	PROPN
american_scientific_journal-2511	12	2	american	american	PROPN
american_scientific_journal-2511	12	3	scientific	scientific	PROPN
american_scientific_journal-2511	12	4	research	research	NOUN
american_scientific_journal-2511	12	5	journal	journal	NOUN
american_scientific_journal-2511	12	6	for	for	ADP
american_scientific_journal-2511	12	7	engineering	engineering	NOUN
american_scientific_journal-2511	12	8	,	,	PUNCT
american_scientific_journal-2511	12	9	technology	technology	NOUN
american_scientific_journal-2511	12	10	,	,	PUNCT
american_scientific_journal-2511	12	11	and	and	CCONJ
american_scientific_journal-2511	12	12	sciences	science	NOUN
american_scientific_journal-2511	12	13	(	(	PUNCT
american_scientific_journal-2511	12	14	asrjets	asrjet	NOUN
american_scientific_journal-2511	12	15	)	)	PUNCT
american_scientific_journal-2511	12	16	(	(	PUNCT
american_scientific_journal-2511	12	17	2017	2017	NUM
american_scientific_journal-2511	12	18	)	)	PUNCT
american_scientific_journal-2511	12	19	volume	volume	NOUN
american_scientific_journal-2511	12	20	27	27	NUM
american_scientific_journal-2511	12	21	,	,	PUNCT
american_scientific_journal-2511	12	22	no	no	DET
american_scientific_journal-2511	12	23	1	1	NUM
american_scientific_journal-2511	12	24	,	,	PUNCT
american_scientific_journal-2511	12	25	pp	pp	ADV
american_scientific_journal-2511	12	26	25	25	NUM
american_scientific_journal-2511	12	27	-	-	SYM
american_scientific_journal-2511	12	28	42	42	NUM
american_scientific_journal-2511	12	29	26	26	NUM
american_scientific_journal-2511	12	30	1	1	NUM
american_scientific_journal-2511	12	31	.	.	PUNCT
american_scientific_journal-2511	13	1	introduction	introduction	NOUN
american_scientific_journal-2511	13	2	the	the	DET
american_scientific_journal-2511	13	3	medicinal	medicinal	ADJ
american_scientific_journal-2511	13	4	leech	leech	NOUN
american_scientific_journal-2511	13	5	is	be	AUX
american_scientific_journal-2511	13	6	a	a	DET
american_scientific_journal-2511	13	7	well	well	ADV
american_scientific_journal-2511	13	8	-	-	PUNCT
american_scientific_journal-2511	13	9	known	know	VERB
american_scientific_journal-2511	13	10	invertebrate	invertebrate	NOUN
american_scientific_journal-2511	13	11	,	,	PUNCT
american_scientific_journal-2511	13	12	widely	widely	ADV
american_scientific_journal-2511	13	13	used	use	VERB
american_scientific_journal-2511	13	14	in	in	ADP
american_scientific_journal-2511	13	15	medicine	medicine	NOUN
american_scientific_journal-2511	13	16	,	,	PUNCT
american_scientific_journal-2511	13	17	for	for	ADP
american_scientific_journal-2511	13	18	many	many	ADJ
american_scientific_journal-2511	13	19	years	year	NOUN
american_scientific_journal-2511	13	20	hirudo	hirudo	NOUN
american_scientific_journal-2511	13	21	medicinalis	medicinali	NOUN
american_scientific_journal-2511	13	22	has	have	AUX
american_scientific_journal-2511	13	23	been	be	AUX
american_scientific_journal-2511	13	24	thought	think	VERB
american_scientific_journal-2511	13	25	to	to	PART
american_scientific_journal-2511	13	26	comprise	comprise	VERB
american_scientific_journal-2511	13	27	a	a	DET
american_scientific_journal-2511	13	28	single	single	ADJ
american_scientific_journal-2511	13	29	species	specie	NOUN
american_scientific_journal-2511	13	30	with	with	ADP
american_scientific_journal-2511	13	31	several	several	ADJ
american_scientific_journal-2511	13	32	different	different	ADJ
american_scientific_journal-2511	13	33	color	color	NOUN
american_scientific_journal-2511	13	34	morph	morph	NOUN
american_scientific_journal-2511	13	35	,	,	PUNCT
american_scientific_journal-2511	13	36	but	but	CCONJ
american_scientific_journal-2511	13	37	recently	recently	ADV
american_scientific_journal-2511	13	38	uncertain	uncertain	ADJ
american_scientific_journal-2511	13	39	according	accord	VERB
american_scientific_journal-2511	13	40	to	to	ADP
american_scientific_journal-2511	13	41	taxonomic	taxonomic	ADJ
american_scientific_journal-2511	13	42	status	status	NOUN
american_scientific_journal-2511	13	43	.	.	PUNCT
american_scientific_journal-2511	14	1	h.	h.	PROPN
american_scientific_journal-2511	14	2	medicinalis	medicinali	NOUN
american_scientific_journal-2511	14	3	actually	actually	ADV
american_scientific_journal-2511	14	4	comprises	comprise	VERB
american_scientific_journal-2511	14	5	several	several	ADJ
american_scientific_journal-2511	14	6	different	different	ADJ
american_scientific_journal-2511	14	7	species	specie	NOUN
american_scientific_journal-2511	14	8	has	have	VERB
american_scientific_journal-2511	14	9	differences	difference	NOUN
american_scientific_journal-2511	14	10	in	in	ADP
american_scientific_journal-2511	14	11	morphology	morphology	NOUN
american_scientific_journal-2511	14	12	,	,	PUNCT
american_scientific_journal-2511	14	13	geographical	geographical	ADJ
american_scientific_journal-2511	14	14	distribution	distribution	NOUN
american_scientific_journal-2511	14	15	and	and	CCONJ
american_scientific_journal-2511	14	16	that	that	PRON
american_scientific_journal-2511	14	17	proved	prove	VERB
american_scientific_journal-2511	14	18	by	by	ADP
american_scientific_journal-2511	14	19	using	use	VERB
american_scientific_journal-2511	14	20	random	random	ADJ
american_scientific_journal-2511	14	21	amplified	amplify	VERB
american_scientific_journal-2511	14	22	polymorphic	polymorphic	ADJ
american_scientific_journal-2511	14	23	dna	dna	NOUN
american_scientific_journal-2511	14	24	technique	technique	NOUN
american_scientific_journal-2511	14	25	(	(	PUNCT
american_scientific_journal-2511	14	26	rapd	rapd	NOUN
american_scientific_journal-2511	14	27	)	)	PUNCT
american_scientific_journal-2511	14	28	,	,	PUNCT
american_scientific_journal-2511	14	29	nuclear	nuclear	ADJ
american_scientific_journal-2511	14	30	18s	18	NOUN
american_scientific_journal-2511	14	31	ribosomal	ribosomal	NOUN
american_scientific_journal-2511	14	32	dna	dna	PROPN
american_scientific_journal-2511	14	33	gene	gene	NOUN
american_scientific_journal-2511	14	34	(	(	PUNCT
american_scientific_journal-2511	14	35	18srdna	18srdna	NUM
american_scientific_journal-2511	14	36	)	)	PUNCT
american_scientific_journal-2511	14	37	and	and	CCONJ
american_scientific_journal-2511	14	38	mitochondrial	mitochondrial	ADJ
american_scientific_journal-2511	14	39	cytochrome	cytochrome	NOUN
american_scientific_journal-2511	14	40	c	c	NOUN
american_scientific_journal-2511	14	41	oxidase	oxidase	NOUN
american_scientific_journal-2511	14	42	subunit	subunit	NOUN
american_scientific_journal-2511	14	43	i	i	PRON
american_scientific_journal-2511	14	44	(	(	PUNCT
american_scientific_journal-2511	14	45	co	co	NOUN
american_scientific_journal-2511	14	46	-	-	PROPN
american_scientific_journal-2511	14	47	i	i	PRON
american_scientific_journal-2511	14	48	)	)	PUNCT
american_scientific_journal-2511	14	49	in	in	ADP
american_scientific_journal-2511	14	50	addition	addition	NOUN
american_scientific_journal-2511	14	51	to	to	ADP
american_scientific_journal-2511	14	52	morphology	morphology	NOUN
american_scientific_journal-2511	14	53	data	datum	NOUN
american_scientific_journal-2511	14	54	which	which	PRON
american_scientific_journal-2511	14	55	led	lead	VERB
american_scientific_journal-2511	14	56	to	to	ADP
american_scientific_journal-2511	14	57	recognition	recognition	NOUN
american_scientific_journal-2511	14	58	of	of	ADP
american_scientific_journal-2511	14	59	four	four	NUM
american_scientific_journal-2511	14	60	distinct	distinct	ADJ
american_scientific_journal-2511	14	61	species	specie	NOUN
american_scientific_journal-2511	14	62	of	of	ADP
american_scientific_journal-2511	14	63	medicinal	medicinal	ADJ
american_scientific_journal-2511	14	64	leeches	leech	NOUN
american_scientific_journal-2511	14	65	:	:	PUNCT
american_scientific_journal-2511	14	66	h.	h.	PROPN
american_scientific_journal-2511	14	67	medicinalis	medicinalis	PROPN
american_scientific_journal-2511	14	68	,	,	PUNCT
american_scientific_journal-2511	14	69	h.	h.	NOUN
american_scientific_journal-2511	14	70	verbena	verbena	NOUN
american_scientific_journal-2511	14	71	,	,	PUNCT
american_scientific_journal-2511	14	72	h.	h.	PROPN
american_scientific_journal-2511	14	73	troctina	troctina	PROPN
american_scientific_journal-2511	14	74	and	and	CCONJ
american_scientific_journal-2511	14	75	h.	h.	PROPN
american_scientific_journal-2511	14	76	orientalis	orientali	NOUN
american_scientific_journal-2511	15	1	[	[	X
american_scientific_journal-2511	15	2	1,2	1,2	NUM
american_scientific_journal-2511	15	3	]	]	PUNCT
american_scientific_journal-2511	15	4	.	.	PUNCT
american_scientific_journal-2511	16	1	the	the	DET
american_scientific_journal-2511	16	2	treatment	treatment	NOUN
american_scientific_journal-2511	16	3	of	of	ADP
american_scientific_journal-2511	16	4	disease	disease	NOUN
american_scientific_journal-2511	16	5	conditions	condition	NOUN
american_scientific_journal-2511	16	6	with	with	ADP
american_scientific_journal-2511	16	7	medicinal	medicinal	ADJ
american_scientific_journal-2511	16	8	leeches	leech	NOUN
american_scientific_journal-2511	16	9	is	be	AUX
american_scientific_journal-2511	16	10	termed	term	VERB
american_scientific_journal-2511	16	11	as	as	ADP
american_scientific_journal-2511	16	12	hirudotherapy	hirudotherapy	PROPN
american_scientific_journal-2511	16	13	(	(	PUNCT
american_scientific_journal-2511	16	14	ht	ht	PROPN
american_scientific_journal-2511	16	15	)	)	PUNCT
american_scientific_journal-2511	16	16	.	.	PUNCT
american_scientific_journal-2511	17	1	ht	ht	PROPN
american_scientific_journal-2511	17	2	takes	take	VERB
american_scientific_journal-2511	17	3	the	the	DET
american_scientific_journal-2511	17	4	advantage	advantage	NOUN
american_scientific_journal-2511	17	5	of	of	ADP
american_scientific_journal-2511	17	6	several	several	ADJ
american_scientific_journal-2511	17	7	biological	biological	ADJ
american_scientific_journal-2511	17	8	properties	property	NOUN
american_scientific_journal-2511	17	9	of	of	ADP
american_scientific_journal-2511	17	10	medicinal	medicinal	ADJ
american_scientific_journal-2511	17	11	leeches	leech	NOUN
american_scientific_journal-2511	17	12	,	,	PUNCT
american_scientific_journal-2511	17	13	among	among	ADP
american_scientific_journal-2511	17	14	these	these	PRON
american_scientific_journal-2511	17	15	,	,	PUNCT
american_scientific_journal-2511	17	16	the	the	DET
american_scientific_journal-2511	17	17	earliest	early	ADJ
american_scientific_journal-2511	17	18	known	know	VERB
american_scientific_journal-2511	17	19	fact	fact	NOUN
american_scientific_journal-2511	17	20	was	be	AUX
american_scientific_journal-2511	17	21	that	that	SCONJ
american_scientific_journal-2511	17	22	leeches	leech	NOUN
american_scientific_journal-2511	17	23	feed	feed	VERB
american_scientific_journal-2511	17	24	on	on	ADP
american_scientific_journal-2511	17	25	the	the	DET
american_scientific_journal-2511	17	26	blood	blood	NOUN
american_scientific_journal-2511	17	27	of	of	ADP
american_scientific_journal-2511	17	28	their	their	PRON
american_scientific_journal-2511	17	29	host	host	NOUN
american_scientific_journal-2511	17	30	and	and	CCONJ
american_scientific_journal-2511	17	31	,	,	PUNCT
american_scientific_journal-2511	17	32	during	during	ADP
american_scientific_journal-2511	17	33	the	the	DET
american_scientific_journal-2511	17	34	course	course	NOUN
american_scientific_journal-2511	17	35	,	,	PUNCT
american_scientific_journal-2511	17	36	release	release	VERB
american_scientific_journal-2511	17	37	pain	pain	NOUN
american_scientific_journal-2511	17	38	-	-	PUNCT
american_scientific_journal-2511	17	39	killing	kill	VERB
american_scientific_journal-2511	17	40	(	(	PUNCT
american_scientific_journal-2511	17	41	anesthetic	anesthetic	NOUN
american_scientific_journal-2511	17	42	)	)	PUNCT
american_scientific_journal-2511	17	43	and	and	CCONJ
american_scientific_journal-2511	17	44	blood	blood	NOUN
american_scientific_journal-2511	17	45	-	-	PUNCT
american_scientific_journal-2511	17	46	thinning	thin	VERB
american_scientific_journal-2511	17	47	substances	substance	NOUN
american_scientific_journal-2511	17	48	(	(	PUNCT
american_scientific_journal-2511	17	49	anticoagulants	anticoagulant	NOUN
american_scientific_journal-2511	17	50	)	)	PUNCT
american_scientific_journal-2511	17	51	along	along	ADP
american_scientific_journal-2511	17	52	with	with	ADP
american_scientific_journal-2511	17	53	their	their	PRON
american_scientific_journal-2511	17	54	saliva	saliva	NOUN
american_scientific_journal-2511	17	55	,	,	PUNCT
american_scientific_journal-2511	17	56	in	in	ADP
american_scientific_journal-2511	17	57	addition	addition	NOUN
american_scientific_journal-2511	17	58	to	to	ADP
american_scientific_journal-2511	17	59	a	a	DET
american_scientific_journal-2511	17	60	cocktail	cocktail	NOUN
american_scientific_journal-2511	17	61	of	of	ADP
american_scientific_journal-2511	17	62	several	several	ADJ
american_scientific_journal-2511	17	63	medicinal	medicinal	ADJ
american_scientific_journal-2511	17	64	molecules	molecule	NOUN
american_scientific_journal-2511	17	65	[	[	X
american_scientific_journal-2511	17	66	3	3	NUM
american_scientific_journal-2511	17	67	]	]	PUNCT
american_scientific_journal-2511	17	68	.	.	PUNCT
american_scientific_journal-2511	18	1	hirudin	hirudin	VERB
american_scientific_journal-2511	18	2	is	be	AUX
american_scientific_journal-2511	18	3	a	a	DET
american_scientific_journal-2511	18	4	naturally	naturally	ADV
american_scientific_journal-2511	18	5	occurring	occur	VERB
american_scientific_journal-2511	18	6	anticoagulant	anticoagulant	ADJ
american_scientific_journal-2511	18	7	protein	protein	NOUN
american_scientific_journal-2511	18	8	,	,	PUNCT
american_scientific_journal-2511	18	9	isolated	isolate	VERB
american_scientific_journal-2511	18	10	from	from	ADP
american_scientific_journal-2511	18	11	the	the	DET
american_scientific_journal-2511	18	12	salivary	salivary	ADJ
american_scientific_journal-2511	18	13	glands	gland	NOUN
american_scientific_journal-2511	18	14	of	of	ADP
american_scientific_journal-2511	18	15	the	the	DET
american_scientific_journal-2511	18	16	medicinal	medicinal	ADJ
american_scientific_journal-2511	18	17	leech	leech	NOUN
american_scientific_journal-2511	18	18	,	,	PUNCT
american_scientific_journal-2511	18	19	it	it	PRON
american_scientific_journal-2511	18	20	's	be	AUX
american_scientific_journal-2511	18	21	a	a	DET
american_scientific_journal-2511	18	22	single	single	ADJ
american_scientific_journal-2511	18	23	chain	chain	NOUN
american_scientific_journal-2511	18	24	poly	poly	ADJ
american_scientific_journal-2511	18	25	peptide	peptide	NOUN
american_scientific_journal-2511	18	26	containing	contain	VERB
american_scientific_journal-2511	18	27	65	65	NUM
american_scientific_journal-2511	18	28	amino	amino	NOUN
american_scientific_journal-2511	18	29	acid	acid	NOUN
american_scientific_journal-2511	18	30	residues	residue	NOUN
american_scientific_journal-2511	18	31	,	,	PUNCT
american_scientific_journal-2511	18	32	which	which	PRON
american_scientific_journal-2511	18	33	contribute	contribute	VERB
american_scientific_journal-2511	18	34	to	to	ADP
american_scientific_journal-2511	18	35	a	a	DET
american_scientific_journal-2511	18	36	molecular	molecular	ADJ
american_scientific_journal-2511	18	37	weight	weight	NOUN
american_scientific_journal-2511	18	38	of	of	ADP
american_scientific_journal-2511	18	39	7000	7000	NUM
american_scientific_journal-2511	18	40	daltons	dalton	NOUN
american_scientific_journal-2511	18	41	hirudin	hirudin	VERB
american_scientific_journal-2511	18	42	consists	consist	NOUN
american_scientific_journal-2511	18	43	of	of	ADP
american_scientific_journal-2511	18	44	six	six	NUM
american_scientific_journal-2511	18	45	cysteine	cysteine	NOUN
american_scientific_journal-2511	18	46	residues	residue	NOUN
american_scientific_journal-2511	18	47	which	which	PRON
american_scientific_journal-2511	18	48	make	make	VERB
american_scientific_journal-2511	18	49	up	up	ADP
american_scientific_journal-2511	18	50	the	the	DET
american_scientific_journal-2511	18	51	three	three	NUM
american_scientific_journal-2511	18	52	disulfide	disulfide	NOUN
american_scientific_journal-2511	18	53	linkages	linkage	NOUN
american_scientific_journal-2511	18	54	in	in	ADP
american_scientific_journal-2511	18	55	the	the	DET
american_scientific_journal-2511	18	56	molecule	molecule	NOUN
american_scientific_journal-2511	18	57	.	.	PUNCT
american_scientific_journal-2511	19	1	the	the	DET
american_scientific_journal-2511	19	2	complete	complete	ADJ
american_scientific_journal-2511	19	3	amino	amino	NOUN
american_scientific_journal-2511	19	4	acid	acid	NOUN
american_scientific_journal-2511	19	5	sequence	sequence	NOUN
american_scientific_journal-2511	19	6	of	of	ADP
american_scientific_journal-2511	19	7	hirudin	hirudin	NOUN
american_scientific_journal-2511	19	8	was	be	AUX
american_scientific_journal-2511	19	9	explained	explain	VERB
american_scientific_journal-2511	19	10	in	in	ADP
american_scientific_journal-2511	19	11	1984	1984	NUM
american_scientific_journal-2511	19	12	by	by	ADP
american_scientific_journal-2511	19	13	dodt	dodt	NOUN
american_scientific_journal-2511	19	14	and	and	CCONJ
american_scientific_journal-2511	19	15	his	his	PRON
american_scientific_journal-2511	19	16	colleagues	colleague	NOUN
american_scientific_journal-2511	19	17	[	[	X
american_scientific_journal-2511	19	18	4,5	4,5	NUM
american_scientific_journal-2511	19	19	]	]	PUNCT
american_scientific_journal-2511	19	20	.	.	PUNCT
american_scientific_journal-2511	20	1	the	the	DET
american_scientific_journal-2511	20	2	molecule	molecule	NOUN
american_scientific_journal-2511	20	3	can	can	AUX
american_scientific_journal-2511	20	4	be	be	AUX
american_scientific_journal-2511	20	5	divided	divide	VERB
american_scientific_journal-2511	20	6	into	into	ADP
american_scientific_journal-2511	20	7	two	two	NUM
american_scientific_journal-2511	20	8	,	,	PUNCT
american_scientific_journal-2511	20	9	the	the	DET
american_scientific_journal-2511	20	10	n(amino	n(amino	NOUN
american_scientific_journal-2511	20	11	)	)	PUNCT
american_scientific_journal-2511	20	12	terminal	terminal	ADJ
american_scientific_journal-2511	20	13	region	region	NOUN
american_scientific_journal-2511	20	14	which	which	PRON
american_scientific_journal-2511	20	15	is	be	AUX
american_scientific_journal-2511	20	16	a	a	DET
american_scientific_journal-2511	20	17	dense	dense	ADJ
american_scientific_journal-2511	20	18	,	,	PUNCT
american_scientific_journal-2511	20	19	hydrophobic	hydrophobic	NOUN
american_scientific_journal-2511	20	20	core	core	NOUN
american_scientific_journal-2511	20	21	region	region	NOUN
american_scientific_journal-2511	20	22	,	,	PUNCT
american_scientific_journal-2511	20	23	containing	contain	VERB
american_scientific_journal-2511	20	24	alternating	alternate	VERB
american_scientific_journal-2511	20	25	polar	polar	ADJ
american_scientific_journal-2511	20	26	and	and	CCONJ
american_scientific_journal-2511	20	27	nonpolar	nonpolar	ADJ
american_scientific_journal-2511	20	28	domains	domain	NOUN
american_scientific_journal-2511	20	29	.	.	PUNCT
american_scientific_journal-2511	21	1	three	three	NUM
american_scientific_journal-2511	21	2	disulfide	disulfide	NOUN
american_scientific_journal-2511	21	3	linkage	linkage	NOUN
american_scientific_journal-2511	21	4	are	be	AUX
american_scientific_journal-2511	21	5	also	also	ADV
american_scientific_journal-2511	21	6	present	present	ADJ
american_scientific_journal-2511	21	7	in	in	ADP
american_scientific_journal-2511	21	8	this	this	DET
american_scientific_journal-2511	21	9	region	region	NOUN
american_scientific_journal-2511	21	10	.	.	PUNCT
american_scientific_journal-2511	22	1	the	the	DET
american_scientific_journal-2511	22	2	c-(carboxy	c-(carboxy	PROPN
american_scientific_journal-2511	22	3	)	)	PUNCT
american_scientific_journal-2511	22	4	terminal	terminal	ADJ
american_scientific_journal-2511	22	5	region	region	NOUN
american_scientific_journal-2511	22	6	,	,	PUNCT
american_scientific_journal-2511	22	7	represents	represent	VERB
american_scientific_journal-2511	22	8	the	the	DET
american_scientific_journal-2511	22	9	hydrophilic	hydrophilic	PROPN
american_scientific_journal-2511	22	10	c	c	PROPN
american_scientific_journal-2511	22	11	terminal	terminal	PROPN
american_scientific_journal-2511	22	12	region	region	NOUN
american_scientific_journal-2511	22	13	.	.	PUNCT
american_scientific_journal-2511	23	1	this	this	DET
american_scientific_journal-2511	23	2	region	region	NOUN
american_scientific_journal-2511	23	3	binds	bind	VERB
american_scientific_journal-2511	23	4	to	to	ADP
american_scientific_journal-2511	23	5	the	the	DET
american_scientific_journal-2511	23	6	fibrinogen	fibrinogen	NOUN
american_scientific_journal-2511	23	7	cleft	cleft	NOUN
american_scientific_journal-2511	23	8	on	on	ADP
american_scientific_journal-2511	23	9	the	the	DET
american_scientific_journal-2511	23	10	thrombin	thrombin	NOUN
american_scientific_journal-2511	23	11	molecule	molecule	NOUN
american_scientific_journal-2511	23	12	and	and	CCONJ
american_scientific_journal-2511	23	13	causes	cause	VERB
american_scientific_journal-2511	23	14	neutralization	neutralization	NOUN
american_scientific_journal-2511	23	15	[	[	X
american_scientific_journal-2511	23	16	4	4	NUM
american_scientific_journal-2511	23	17	]	]	PUNCT
american_scientific_journal-2511	23	18	.	.	PUNCT
american_scientific_journal-2511	24	1	hirudin	hirudin	AUX
american_scientific_journal-2511	24	2	classified	classify	VERB
american_scientific_journal-2511	24	3	as	as	ADP
american_scientific_journal-2511	24	4	anticoagulant	anticoagulant	ADJ
american_scientific_journal-2511	24	5	drug	drug	NOUN
american_scientific_journal-2511	24	6	,	,	PUNCT
american_scientific_journal-2511	24	7	specifically	specifically	ADV
american_scientific_journal-2511	24	8	its	its	PRON
american_scientific_journal-2511	24	9	considered	consider	VERB
american_scientific_journal-2511	24	10	as	as	ADP
american_scientific_journal-2511	24	11	direct	direct	ADJ
american_scientific_journal-2511	24	12	thrombin	thrombin	NOUN
american_scientific_journal-2511	24	13	inhibitors	inhibitor	NOUN
american_scientific_journal-2511	24	14	(	(	PUNCT
american_scientific_journal-2511	24	15	dtis	dtis	NOUN
american_scientific_journal-2511	24	16	)	)	PUNCT
american_scientific_journal-2511	24	17	,	,	PUNCT
american_scientific_journal-2511	24	18	these	these	DET
american_scientific_journal-2511	24	19	classes	class	NOUN
american_scientific_journal-2511	24	20	of	of	ADP
american_scientific_journal-2511	24	21	drug	drug	NOUN
american_scientific_journal-2511	24	22	bind	bind	VERB
american_scientific_journal-2511	24	23	thrombin	thrombin	NOUN
american_scientific_journal-2511	24	24	directly	directly	ADV
american_scientific_journal-2511	24	25	and	and	CCONJ
american_scientific_journal-2511	24	26	block	block	VERB
american_scientific_journal-2511	24	27	its	its	PRON
american_scientific_journal-2511	24	28	interaction	interaction	NOUN
american_scientific_journal-2511	24	29	with	with	ADP
american_scientific_journal-2511	24	30	its	its	PRON
american_scientific_journal-2511	24	31	substrates	substrate	NOUN
american_scientific_journal-2511	24	32	[	[	X
american_scientific_journal-2511	24	33	6	6	NUM
american_scientific_journal-2511	24	34	]	]	PUNCT
american_scientific_journal-2511	24	35	.	.	PUNCT
american_scientific_journal-2511	25	1	molecular	molecular	ADJ
american_scientific_journal-2511	25	2	engineering	engineering	NOUN
american_scientific_journal-2511	25	3	techniques	technique	NOUN
american_scientific_journal-2511	25	4	have	have	AUX
american_scientific_journal-2511	25	5	now	now	ADV
american_scientific_journal-2511	25	6	made	make	VERB
american_scientific_journal-2511	25	7	it	it	PRON
american_scientific_journal-2511	25	8	possible	possible	ADJ
american_scientific_journal-2511	25	9	to	to	PART
american_scientific_journal-2511	25	10	obtain	obtain	VERB
american_scientific_journal-2511	25	11	several	several	ADJ
american_scientific_journal-2511	25	12	recombinant	recombinant	ADJ
american_scientific_journal-2511	25	13	variants	variant	NOUN
american_scientific_journal-2511	25	14	of	of	ADP
american_scientific_journal-2511	25	15	hirudin	hirudin	NOUN
american_scientific_journal-2511	25	16	,	,	PUNCT
american_scientific_journal-2511	25	17	three	three	NUM
american_scientific_journal-2511	25	18	are	be	AUX
american_scientific_journal-2511	25	19	easily	easily	ADV
american_scientific_journal-2511	25	20	produced	produce	VERB
american_scientific_journal-2511	25	21	rhv1	rhv1	NOUN
american_scientific_journal-2511	25	22	,	,	PUNCT
american_scientific_journal-2511	25	23	rhv2	rhv2	PROPN
american_scientific_journal-2511	25	24	,	,	PUNCT
american_scientific_journal-2511	25	25	and	and	CCONJ
american_scientific_journal-2511	25	26	rhv3	rhv3	PROPN
american_scientific_journal-2511	25	27	,	,	PUNCT
american_scientific_journal-2511	25	28	according	accord	VERB
american_scientific_journal-2511	25	29	to	to	ADP
american_scientific_journal-2511	25	30	the	the	DET
american_scientific_journal-2511	25	31	natural	natural	ADJ
american_scientific_journal-2511	25	32	variant	variant	NOUN
american_scientific_journal-2511	25	33	hv1	hv1	NOUN
american_scientific_journal-2511	25	34	,	,	PUNCT
american_scientific_journal-2511	25	35	hv2	hv2	PROPN
american_scientific_journal-2511	25	36	and	and	CCONJ
american_scientific_journal-2511	25	37	hv3	hv3	PRON
american_scientific_journal-2511	26	1	[	[	X
american_scientific_journal-2511	26	2	7	7	NUM
american_scientific_journal-2511	26	3	]	]	PUNCT
american_scientific_journal-2511	26	4	.	.	PUNCT
american_scientific_journal-2511	27	1	beside	beside	ADP
american_scientific_journal-2511	27	2	the	the	DET
american_scientific_journal-2511	27	3	hirudin	hirudin	NOUN
american_scientific_journal-2511	27	4	extraction	extraction	NOUN
american_scientific_journal-2511	27	5	from	from	ADP
american_scientific_journal-2511	27	6	hirudo	hirudo	NOUN
american_scientific_journal-2511	27	7	sp	sp	PROPN
american_scientific_journal-2511	27	8	.	.	PROPN
american_scientific_journal-2511	27	9	requires	require	VERB
american_scientific_journal-2511	27	10	killing	kill	VERB
american_scientific_journal-2511	27	11	a	a	DET
american_scientific_journal-2511	27	12	large	large	ADJ
american_scientific_journal-2511	27	13	number	number	NOUN
american_scientific_journal-2511	27	14	of	of	ADP
american_scientific_journal-2511	27	15	leeches	leech	NOUN
american_scientific_journal-2511	27	16	,	,	PUNCT
american_scientific_journal-2511	27	17	like	like	ADP
american_scientific_journal-2511	27	18	that	that	PRON
american_scientific_journal-2511	27	19	in	in	ADP
american_scientific_journal-2511	27	20	europe	europe	PROPN
american_scientific_journal-2511	27	21	about	about	ADP
american_scientific_journal-2511	27	22	12000	12000	NUM
american_scientific_journal-2511	27	23	kg	kg	NOUN
american_scientific_journal-2511	27	24	of	of	ADP
american_scientific_journal-2511	27	25	leeches	leech	NOUN
american_scientific_journal-2511	27	26	being	be	AUX
american_scientific_journal-2511	27	27	consumed	consume	VERB
american_scientific_journal-2511	27	28	every	every	DET
american_scientific_journal-2511	27	29	year	year	NOUN
american_scientific_journal-2511	27	30	for	for	ADP
american_scientific_journal-2511	27	31	the	the	DET
american_scientific_journal-2511	27	32	purpose	purpose	NOUN
american_scientific_journal-2511	27	33	of	of	ADP
american_scientific_journal-2511	27	34	production	production	NOUN
american_scientific_journal-2511	27	35	,	,	PUNCT
american_scientific_journal-2511	27	36	also	also	ADV
american_scientific_journal-2511	27	37	,	,	PUNCT
american_scientific_journal-2511	27	38	many	many	ADJ
american_scientific_journal-2511	27	39	pharmaceutical	pharmaceutical	ADJ
american_scientific_journal-2511	27	40	companies	company	NOUN
american_scientific_journal-2511	27	41	today	today	NOUN
american_scientific_journal-2511	27	42	produce	produce	VERB
american_scientific_journal-2511	27	43	manufactured	manufacture	VERB
american_scientific_journal-2511	27	44	hirudin	hirudin	NOUN
american_scientific_journal-2511	27	45	compounds	compound	NOUN
american_scientific_journal-2511	27	46	but	but	CCONJ
american_scientific_journal-2511	27	47	with	with	ADP
american_scientific_journal-2511	27	48	high	high	ADJ
american_scientific_journal-2511	27	49	cost	cost	NOUN
american_scientific_journal-2511	27	50	,	,	PUNCT
american_scientific_journal-2511	27	51	moreover	moreover	ADV
american_scientific_journal-2511	27	52	,	,	PUNCT
american_scientific_journal-2511	27	53	the	the	DET
american_scientific_journal-2511	27	54	gene	gene	NOUN
american_scientific_journal-2511	27	55	responsible	responsible	ADJ
american_scientific_journal-2511	27	56	for	for	ADP
american_scientific_journal-2511	27	57	hirudin	hirudin	NOUN
american_scientific_journal-2511	27	58	was	be	AUX
american_scientific_journal-2511	27	59	fused	fuse	VERB
american_scientific_journal-2511	27	60	with	with	ADP
american_scientific_journal-2511	27	61	other	other	ADJ
american_scientific_journal-2511	27	62	protein	protein	NOUN
american_scientific_journal-2511	27	63	genes	gene	NOUN
american_scientific_journal-2511	27	64	and	and	CCONJ
american_scientific_journal-2511	27	65	expressed	express	VERB
american_scientific_journal-2511	27	66	in	in	ADP
american_scientific_journal-2511	27	67	bacteria	bacteria	NOUN
american_scientific_journal-2511	27	68	(	(	PUNCT
american_scientific_journal-2511	27	69	escherichia	escherichia	NOUN
american_scientific_journal-2511	27	70	coli	coli	NOUN
american_scientific_journal-2511	27	71	or	or	CCONJ
american_scientific_journal-2511	27	72	bacillus	bacillus	NOUN
american_scientific_journal-2511	27	73	subtilis	subtili	NOUN
american_scientific_journal-2511	27	74	)	)	PUNCT
american_scientific_journal-2511	27	75	or	or	CCONJ
american_scientific_journal-2511	27	76	yeast	yeast	NOUN
american_scientific_journal-2511	27	77	cell	cell	NOUN
american_scientific_journal-2511	27	78	like	like	ADP
american_scientific_journal-2511	27	79	saccharomyces	saccharomyce	NOUN
american_scientific_journal-2511	27	80	cerevisae	cerevisae	NOUN
american_scientific_journal-2511	27	81	or	or	CCONJ
american_scientific_journal-2511	27	82	pichia	pichia	NOUN
american_scientific_journal-2511	27	83	pastoris	pastori	NOUN
american_scientific_journal-2511	27	84	)	)	PUNCT
american_scientific_journal-2511	27	85	,	,	PUNCT
american_scientific_journal-2511	27	86	thus	thus	ADV
american_scientific_journal-2511	27	87	the	the	DET
american_scientific_journal-2511	27	88	recombinant	recombinant	ADJ
american_scientific_journal-2511	27	89	dna	dna	NOUN
american_scientific_journal-2511	27	90	technology	technology	NOUN
american_scientific_journal-2511	27	91	provides	provide	VERB
american_scientific_journal-2511	27	92	a	a	DET
american_scientific_journal-2511	27	93	mean	mean	NOUN
american_scientific_journal-2511	27	94	to	to	ADP
american_scientific_journal-2511	27	95	a	a	DET
american_scientific_journal-2511	27	96	produce	produce	NOUN
american_scientific_journal-2511	27	97	r	r	NOUN
american_scientific_journal-2511	27	98	-	-	PUNCT
american_scientific_journal-2511	27	99	hirudin	hirudin	VERB
american_scientific_journal-2511	27	100	on	on	ADP
american_scientific_journal-2511	27	101	a	a	DET
american_scientific_journal-2511	27	102	large	large	ADJ
american_scientific_journal-2511	27	103	scale	scale	NOUN
american_scientific_journal-2511	27	104	for	for	ADP
american_scientific_journal-2511	27	105	the	the	DET
american_scientific_journal-2511	27	106	use	use	NOUN
american_scientific_journal-2511	27	107	in	in	ADP
american_scientific_journal-2511	27	108	clinical	clinical	ADJ
american_scientific_journal-2511	27	109	medicine	medicine	NOUN
american_scientific_journal-2511	27	110	.	.	PUNCT
american_scientific_journal-2511	28	1	the	the	DET
american_scientific_journal-2511	28	2	large	large	ADJ
american_scientific_journal-2511	28	3	-	-	PUNCT
american_scientific_journal-2511	28	4	scale	scale	NOUN
american_scientific_journal-2511	28	5	production	production	NOUN
american_scientific_journal-2511	28	6	of	of	ADP
american_scientific_journal-2511	28	7	bacterial	bacterial	ADJ
american_scientific_journal-2511	28	8	or	or	CCONJ
american_scientific_journal-2511	28	9	yeast	yeast	NOUN
american_scientific_journal-2511	28	10	hirudin	hirudin	NOUN
american_scientific_journal-2511	28	11	would	would	AUX
american_scientific_journal-2511	28	12	save	save	VERB
american_scientific_journal-2511	28	13	the	the	DET
american_scientific_journal-2511	28	14	lives	life	NOUN
american_scientific_journal-2511	28	15	of	of	ADP
american_scientific_journal-2511	28	16	large	large	ADJ
american_scientific_journal-2511	28	17	numbers	number	NOUN
american_scientific_journal-2511	28	18	of	of	ADP
american_scientific_journal-2511	28	19	medicinal	medicinal	ADJ
american_scientific_journal-2511	28	20	leeches	leech	NOUN
american_scientific_journal-2511	28	21	[	[	X
american_scientific_journal-2511	28	22	8	8	NUM
american_scientific_journal-2511	28	23	]	]	PUNCT
american_scientific_journal-2511	28	24	.	.	PUNCT
american_scientific_journal-2511	29	1	thus	thus	ADV
american_scientific_journal-2511	29	2	,	,	PUNCT
american_scientific_journal-2511	29	3	the	the	DET
american_scientific_journal-2511	29	4	aim	aim	NOUN
american_scientific_journal-2511	29	5	of	of	ADP
american_scientific_journal-2511	29	6	this	this	DET
american_scientific_journal-2511	29	7	study	study	NOUN
american_scientific_journal-2511	29	8	was	be	AUX
american_scientific_journal-2511	29	9	production	production	NOUN
american_scientific_journal-2511	29	10	of	of	ADP
american_scientific_journal-2511	29	11	anticoagulant	anticoagulant	ADJ
american_scientific_journal-2511	29	12	drug	drug	NOUN
american_scientific_journal-2511	29	13	(	(	PUNCT
american_scientific_journal-2511	29	14	hirudin	hirudin	NOUN
american_scientific_journal-2511	29	15	)	)	PUNCT
american_scientific_journal-2511	29	16	from	from	ADP
american_scientific_journal-2511	29	17	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2511	29	18	)	)	PUNCT
american_scientific_journal-2511	29	19	strain	strain	NOUN
american_scientific_journal-2511	29	20	by	by	ADP
american_scientific_journal-2511	29	21	genetic	genetic	ADJ
american_scientific_journal-2511	29	22	engineering	engineering	NOUN
american_scientific_journal-2511	29	23	methods	method	NOUN
american_scientific_journal-2511	29	24	.	.	PUNCT
american_scientific_journal-2511	30	1	2	2	X
american_scientific_journal-2511	30	2	.	.	X
american_scientific_journal-2511	30	3	materials	material	NOUN
american_scientific_journal-2511	30	4	and	and	CCONJ
american_scientific_journal-2511	30	5	methods	method	NOUN
american_scientific_journal-2511	30	6	2.1leeches	2.1leeches	NUM
american_scientific_journal-2511	30	7	collection	collection	NOUN
american_scientific_journal-2511	30	8	live	live	ADJ
american_scientific_journal-2511	30	9	medical	medical	ADJ
american_scientific_journal-2511	30	10	leeches	leech	NOUN
american_scientific_journal-2511	30	11	were	be	AUX
american_scientific_journal-2511	30	12	collected	collect	VERB
american_scientific_journal-2511	30	13	from	from	ADP
american_scientific_journal-2511	30	14	the	the	DET
american_scientific_journal-2511	30	15	islamic	islamic	PROPN
american_scientific_journal-2511	30	16	republic	republic	PROPN
american_scientific_journal-2511	30	17	of	of	ADP
american_scientific_journal-2511	30	18	iran	iran	PROPN
american_scientific_journal-2511	30	19	/	/	SYM
american_scientific_journal-2511	30	20	province	province	PROPN
american_scientific_journal-2511	30	21	khuzestankhorramshahr	khuzestankhorramshahr	PROPN
american_scientific_journal-2511	30	22	american	american	PROPN
american_scientific_journal-2511	30	23	scientific	scientific	PROPN
american_scientific_journal-2511	30	24	research	research	PROPN
american_scientific_journal-2511	30	25	journal	journal	NOUN
american_scientific_journal-2511	30	26	for	for	ADP
american_scientific_journal-2511	30	27	engineering	engineering	NOUN
american_scientific_journal-2511	30	28	,	,	PUNCT
american_scientific_journal-2511	30	29	technology	technology	NOUN
american_scientific_journal-2511	30	30	,	,	PUNCT
american_scientific_journal-2511	30	31	and	and	CCONJ
american_scientific_journal-2511	30	32	sciences	science	NOUN
american_scientific_journal-2511	30	33	(	(	PUNCT
american_scientific_journal-2511	30	34	asrjets	asrjet	NOUN
american_scientific_journal-2511	30	35	)	)	PUNCT
american_scientific_journal-2511	30	36	(	(	PUNCT
american_scientific_journal-2511	30	37	2017	2017	NUM
american_scientific_journal-2511	30	38	)	)	PUNCT
american_scientific_journal-2511	30	39	volume	volume	NOUN
american_scientific_journal-2511	30	40	27	27	NUM
american_scientific_journal-2511	30	41	,	,	PUNCT
american_scientific_journal-2511	30	42	no	no	DET
american_scientific_journal-2511	30	43	1	1	NUM
american_scientific_journal-2511	30	44	,	,	PUNCT
american_scientific_journal-2511	30	45	pp	pp	ADV
american_scientific_journal-2511	30	46	25	25	NUM
american_scientific_journal-2511	30	47	-	-	SYM
american_scientific_journal-2511	30	48	42	42	NUM
american_scientific_journal-2511	30	49	27	27	NUM
american_scientific_journal-2511	30	50	(	(	PUNCT
american_scientific_journal-2511	30	51	mohammerah	mohammerah	PROPN
american_scientific_journal-2511	30	52	)	)	PUNCT
american_scientific_journal-2511	30	53	.	.	PUNCT
american_scientific_journal-2511	31	1	medical	medical	ADJ
american_scientific_journal-2511	31	2	leeches	leech	NOUN
american_scientific_journal-2511	31	3	were	be	AUX
american_scientific_journal-2511	31	4	cut	cut	VERB
american_scientific_journal-2511	31	5	into	into	ADP
american_scientific_journal-2511	31	6	pieces	piece	NOUN
american_scientific_journal-2511	31	7	and	and	CCONJ
american_scientific_journal-2511	31	8	rolled	roll	VERB
american_scientific_journal-2511	31	9	by	by	ADP
american_scientific_journal-2511	31	10	aluminum	aluminum	NOUN
american_scientific_journal-2511	31	11	foil	foil	NOUN
american_scientific_journal-2511	31	12	then	then	ADV
american_scientific_journal-2511	31	13	placed	place	VERB
american_scientific_journal-2511	31	14	directly	directly	ADV
american_scientific_journal-2511	31	15	into	into	ADP
american_scientific_journal-2511	31	16	liquid	liquid	ADJ
american_scientific_journal-2511	31	17	nitrogen	nitrogen	NOUN
american_scientific_journal-2511	31	18	then	then	ADV
american_scientific_journal-2511	31	19	,	,	PUNCT
american_scientific_journal-2511	31	20	these	these	DET
american_scientific_journal-2511	31	21	pieces	piece	NOUN
american_scientific_journal-2511	31	22	’	'	PUNCT
american_scientific_journal-2511	31	23	grind	grind	NOUN
american_scientific_journal-2511	31	24	into	into	ADP
american_scientific_journal-2511	31	25	fine	fine	ADJ
american_scientific_journal-2511	31	26	powder	powder	NOUN
american_scientific_journal-2511	31	27	by	by	ADP
american_scientific_journal-2511	31	28	a	a	DET
american_scientific_journal-2511	31	29	pestle	pestle	NOUN
american_scientific_journal-2511	31	30	in	in	ADP
american_scientific_journal-2511	31	31	mortar	mortar	NOUN
american_scientific_journal-2511	31	32	filled	fill	VERB
american_scientific_journal-2511	31	33	by	by	ADP
american_scientific_journal-2511	31	34	liquid	liquid	ADJ
american_scientific_journal-2511	31	35	nitrogen	nitrogen	NOUN
american_scientific_journal-2511	31	36	and	and	CCONJ
american_scientific_journal-2511	31	37	used	use	VERB
american_scientific_journal-2511	31	38	directly	directly	ADV
american_scientific_journal-2511	31	39	to	to	PART
american_scientific_journal-2511	31	40	extract	extract	VERB
american_scientific_journal-2511	31	41	rna	rna	NOUN
american_scientific_journal-2511	31	42	and	and	CCONJ
american_scientific_journal-2511	31	43	dna	dna	PROPN
american_scientific_journal-2511	31	44	,	,	PUNCT
american_scientific_journal-2511	31	45	while	while	SCONJ
american_scientific_journal-2511	31	46	the	the	DET
american_scientific_journal-2511	31	47	rest	rest	NOUN
american_scientific_journal-2511	31	48	was	be	AUX
american_scientific_journal-2511	31	49	saved	save	VERB
american_scientific_journal-2511	31	50	in	in	ADP
american_scientific_journal-2511	31	51	deep	deep	ADJ
american_scientific_journal-2511	31	52	freeze	freeze	NOUN
american_scientific_journal-2511	31	53	.	.	PUNCT
american_scientific_journal-2511	32	1	2.2	2.2	NUM
american_scientific_journal-2511	32	2	classification	classification	NOUN
american_scientific_journal-2511	32	3	of	of	ADP
american_scientific_journal-2511	32	4	leeches	leech	NOUN
american_scientific_journal-2511	32	5	genomic	genomic	NOUN
american_scientific_journal-2511	32	6	dna	dna	PROPN
american_scientific_journal-2511	32	7	was	be	AUX
american_scientific_journal-2511	32	8	extracted	extract	VERB
american_scientific_journal-2511	32	9	from	from	ADP
american_scientific_journal-2511	32	10	leeches	leech	NOUN
american_scientific_journal-2511	32	11	using	use	VERB
american_scientific_journal-2511	32	12	gsync	gsync	NOUN
american_scientific_journal-2511	32	13	™	™	ADJ
american_scientific_journal-2511	32	14	dna	dna	NOUN
american_scientific_journal-2511	32	15	extraction	extraction	NOUN
american_scientific_journal-2511	32	16	kit	kit	NOUN
american_scientific_journal-2511	32	17	(	(	PUNCT
american_scientific_journal-2511	32	18	geneaid	geneaid	PROPN
american_scientific_journal-2511	32	19	,	,	PUNCT
american_scientific_journal-2511	32	20	tiawan	tiawan	PROPN
american_scientific_journal-2511	32	21	)	)	PUNCT
american_scientific_journal-2511	32	22	catalog	catalog	NOUN
american_scientific_journal-2511	32	23	no	no	INTJ
american_scientific_journal-2511	32	24	.	.	PUNCT
american_scientific_journal-2511	33	1	gs100	gs100	NOUN
american_scientific_journal-2511	33	2	.	.	PUNCT
american_scientific_journal-2511	34	1	molecular	molecular	ADJ
american_scientific_journal-2511	34	2	characters	character	NOUN
american_scientific_journal-2511	34	3	have	have	AUX
american_scientific_journal-2511	34	4	been	be	AUX
american_scientific_journal-2511	34	5	obtained	obtain	VERB
american_scientific_journal-2511	34	6	for	for	ADP
american_scientific_journal-2511	34	7	the	the	DET
american_scientific_journal-2511	34	8	phylogenetic	phylogenetic	ADJ
american_scientific_journal-2511	34	9	inference	inference	NOUN
american_scientific_journal-2511	34	10	from	from	ADP
american_scientific_journal-2511	34	11	the	the	DET
american_scientific_journal-2511	34	12	nuclear	nuclear	ADJ
american_scientific_journal-2511	34	13	small	small	ADJ
american_scientific_journal-2511	34	14	subunit	subunit	NOUN
american_scientific_journal-2511	34	15	18s	18	NOUN
american_scientific_journal-2511	34	16	r	r	NOUN
american_scientific_journal-2511	34	17	dna	dna	PROPN
american_scientific_journal-2511	34	18	gene	gene	NOUN
american_scientific_journal-2511	34	19	by	by	ADP
american_scientific_journal-2511	34	20	using	use	VERB
american_scientific_journal-2511	34	21	the	the	DET
american_scientific_journal-2511	34	22	following	follow	VERB
american_scientific_journal-2511	34	23	primers	primer	NOUN
american_scientific_journal-2511	34	24	:	:	PUNCT
american_scientific_journal-2511	34	25	d1	d1	NOUN
american_scientific_journal-2511	34	26	(	(	PUNCT
american_scientific_journal-2511	34	27	5´-aacctggttgatcctgccagt-3	5´-aacctggttgatcctgccagt-3	NUM
american_scientific_journal-2511	34	28	´	´	NOUN
american_scientific_journal-2511	34	29	)	)	PUNCT
american_scientific_journal-2511	34	30	and	and	CCONJ
american_scientific_journal-2511	34	31	d2	d2	PROPN
american_scientific_journal-2511	34	32	(	(	PUNCT
american_scientific_journal-2511	34	33	5´tgatccttccgcag	5´tgatccttccgcag	NUM
american_scientific_journal-2511	34	34	gttcacct-3	gttcacct-3	NUM
american_scientific_journal-2511	34	35	´	´	NOUN
american_scientific_journal-2511	34	36	)	)	PUNCT
american_scientific_journal-2511	34	37	and	and	CCONJ
american_scientific_journal-2511	34	38	from	from	ADP
american_scientific_journal-2511	34	39	cytochrome	cytochrome	PROPN
american_scientific_journal-2511	34	40	c	c	NOUN
american_scientific_journal-2511	34	41	oxidase	oxidase	NOUN
american_scientific_journal-2511	34	42	i	i	PRON
american_scientific_journal-2511	34	43	(	(	PUNCT
american_scientific_journal-2511	34	44	co	co	NOUN
american_scientific_journal-2511	34	45	-	-	PROPN
american_scientific_journal-2511	34	46	i	i	NOUN
american_scientific_journal-2511	34	47	)	)	PUNCT
american_scientific_journal-2511	34	48	gene	gene	NOUN
american_scientific_journal-2511	34	49	by	by	ADP
american_scientific_journal-2511	34	50	using	use	VERB
american_scientific_journal-2511	34	51	the	the	DET
american_scientific_journal-2511	34	52	m1(5´ggtcaacaaatcataaagatattgg-3	m1(5´ggtcaacaaatcataaagatattgg-3	NOUN
american_scientific_journal-2511	34	53	´	´	NOUN
american_scientific_journal-2511	34	54	)	)	PUNCT
american_scientific_journal-2511	34	55	and	and	CCONJ
american_scientific_journal-2511	34	56	m2(5´-taaacttcagggtgaccaaaaaatca-3	m2(5´-taaacttcagggtgaccaaaaaatca-3	NOUN
american_scientific_journal-2511	34	57	´	´	NOUN
american_scientific_journal-2511	34	58	)	)	PUNCT
american_scientific_journal-2511	34	59	primers	primer	NOUN
american_scientific_journal-2511	34	60	.	.	PUNCT
american_scientific_journal-2511	35	1	gene	gene	NOUN
american_scientific_journal-2511	35	2	amplification	amplification	NOUN
american_scientific_journal-2511	35	3	was	be	AUX
american_scientific_journal-2511	35	4	performed	perform	VERB
american_scientific_journal-2511	35	5	in	in	ADP
american_scientific_journal-2511	35	6	a	a	DET
american_scientific_journal-2511	35	7	final	final	ADJ
american_scientific_journal-2511	35	8	volume	volume	NOUN
american_scientific_journal-2511	35	9	of	of	ADP
american_scientific_journal-2511	35	10	25µl	25µl	NOUN
american_scientific_journal-2511	35	11	of	of	ADP
american_scientific_journal-2511	35	12	reaction	reaction	NOUN
american_scientific_journal-2511	35	13	mixtures	mixture	NOUN
american_scientific_journal-2511	35	14	contained	contain	VERB
american_scientific_journal-2511	35	15	5µl	5µl	ADJ
american_scientific_journal-2511	35	16	dna	dna	NOUN
american_scientific_journal-2511	35	17	template	template	NOUN
american_scientific_journal-2511	35	18	,	,	PUNCT
american_scientific_journal-2511	35	19	1µ	1µ	NUM
american_scientific_journal-2511	35	20	d1	d1	PROPN
american_scientific_journal-2511	35	21	or	or	CCONJ
american_scientific_journal-2511	35	22	m1	m1	PROPN
american_scientific_journal-2511	35	23	primer	primer	NOUN
american_scientific_journal-2511	35	24	,	,	PUNCT
american_scientific_journal-2511	35	25	1µl	1µl	ADJ
american_scientific_journal-2511	35	26	d2	d2	PROPN
american_scientific_journal-2511	35	27	or	or	CCONJ
american_scientific_journal-2511	35	28	m2	m2	PROPN
american_scientific_journal-2511	35	29	primer	primer	NOUN
american_scientific_journal-2511	35	30	,	,	PUNCT
american_scientific_journal-2511	35	31	12.5	12.5	NUM
american_scientific_journal-2511	35	32	pcr	pcr	NOUN
american_scientific_journal-2511	35	33	master	master	NOUN
american_scientific_journal-2511	35	34	mix	mix	NOUN
american_scientific_journal-2511	35	35	promega	promega	PROPN
american_scientific_journal-2511	35	36	and	and	CCONJ
american_scientific_journal-2511	35	37	5.5µl	5.5µl	NUM
american_scientific_journal-2511	35	38	nuclease	nuclease	NOUN
american_scientific_journal-2511	35	39	free	free	ADJ
american_scientific_journal-2511	35	40	water	water	NOUN
american_scientific_journal-2511	35	41	.	.	PUNCT
american_scientific_journal-2511	36	1	the	the	DET
american_scientific_journal-2511	36	2	pcr	pcr	ADJ
american_scientific_journal-2511	36	3	condition	condition	NOUN
american_scientific_journal-2511	36	4	for	for	ADP
american_scientific_journal-2511	36	5	18s	18	NOUN
american_scientific_journal-2511	36	6	rdna	rdna	NOUN
american_scientific_journal-2511	36	7	gene	gene	NOUN
american_scientific_journal-2511	36	8	were	be	AUX
american_scientific_journal-2511	36	9	:	:	PUNCT
american_scientific_journal-2511	36	10	initial	initial	ADJ
american_scientific_journal-2511	36	11	denaturation	denaturation	NOUN
american_scientific_journal-2511	36	12	at	at	ADP
american_scientific_journal-2511	36	13	94	94	NUM
american_scientific_journal-2511	36	14	c	c	NOUN
american_scientific_journal-2511	36	15	°	°	NUM
american_scientific_journal-2511	36	16	for	for	ADP
american_scientific_journal-2511	36	17	4min	4min	NOUN
american_scientific_journal-2511	36	18	.	.	PROPN
american_scientific_journal-2511	36	19	followed	follow	VERB
american_scientific_journal-2511	36	20	by	by	ADP
american_scientific_journal-2511	36	21	35	35	NUM
american_scientific_journal-2511	36	22	cycle	cycle	NOUN
american_scientific_journal-2511	36	23	of	of	ADP
american_scientific_journal-2511	36	24	denaturation	denaturation	NOUN
american_scientific_journal-2511	36	25	at	at	ADP
american_scientific_journal-2511	36	26	94	94	NUM
american_scientific_journal-2511	36	27	c	c	NOUN
american_scientific_journal-2511	36	28	°	°	NUM
american_scientific_journal-2511	36	29	for	for	ADP
american_scientific_journal-2511	36	30	20	20	NUM
american_scientific_journal-2511	36	31	sec	sec	PROPN
american_scientific_journal-2511	36	32	.	.	PROPN
american_scientific_journal-2511	36	33	,	,	PUNCT
american_scientific_journal-2511	36	34	annealing	anneal	VERB
american_scientific_journal-2511	36	35	at	at	ADP
american_scientific_journal-2511	36	36	47	47	NUM
american_scientific_journal-2511	36	37	c	c	NOUN
american_scientific_journal-2511	36	38	°	°	NUM
american_scientific_journal-2511	36	39	for	for	ADP
american_scientific_journal-2511	36	40	20	20	NUM
american_scientific_journal-2511	36	41	sec	sec	PROPN
american_scientific_journal-2511	36	42	.	.	PROPN
american_scientific_journal-2511	37	1	and	and	CCONJ
american_scientific_journal-2511	37	2	extension	extension	NOUN
american_scientific_journal-2511	37	3	at	at	ADP
american_scientific_journal-2511	37	4	68c	68c	NOUN
american_scientific_journal-2511	37	5	°	°	NOUN
american_scientific_journal-2511	37	6	for	for	ADP
american_scientific_journal-2511	37	7	105	105	NUM
american_scientific_journal-2511	37	8	sec	sec	PROPN
american_scientific_journal-2511	37	9	.	.	PROPN
american_scientific_journal-2511	38	1	with	with	ADP
american_scientific_journal-2511	38	2	final	final	ADJ
american_scientific_journal-2511	38	3	extension	extension	NOUN
american_scientific_journal-2511	38	4	at	at	ADP
american_scientific_journal-2511	38	5	72	72	NUM
american_scientific_journal-2511	38	6	c	c	NOUN
american_scientific_journal-2511	38	7	°	°	NOUN
american_scientific_journal-2511	38	8	for	for	ADP
american_scientific_journal-2511	38	9	7min	7min	NUM
american_scientific_journal-2511	38	10	.	.	PUNCT
american_scientific_journal-2511	39	1	whereas	whereas	ADV
american_scientific_journal-2511	39	2	,	,	PUNCT
american_scientific_journal-2511	39	3	the	the	DET
american_scientific_journal-2511	39	4	pcr	pcr	ADJ
american_scientific_journal-2511	39	5	condition	condition	NOUN
american_scientific_journal-2511	39	6	for	for	ADP
american_scientific_journal-2511	39	7	coi	coi	NOUN
american_scientific_journal-2511	39	8	gene	gene	NOUN
american_scientific_journal-2511	39	9	were	be	AUX
american_scientific_journal-2511	39	10	:	:	PUNCT
american_scientific_journal-2511	39	11	initial	initial	ADJ
american_scientific_journal-2511	39	12	denaturation	denaturation	NOUN
american_scientific_journal-2511	39	13	at	at	ADP
american_scientific_journal-2511	39	14	94	94	NUM
american_scientific_journal-2511	39	15	c	c	NOUN
american_scientific_journal-2511	39	16	°	°	NUM
american_scientific_journal-2511	39	17	for	for	ADP
american_scientific_journal-2511	39	18	4min	4min	NOUN
american_scientific_journal-2511	39	19	.	.	PROPN
american_scientific_journal-2511	39	20	followed	follow	VERB
american_scientific_journal-2511	39	21	by	by	ADP
american_scientific_journal-2511	39	22	35	35	NUM
american_scientific_journal-2511	39	23	cycle	cycle	NOUN
american_scientific_journal-2511	39	24	of	of	ADP
american_scientific_journal-2511	39	25	denaturation	denaturation	NOUN
american_scientific_journal-2511	39	26	at	at	ADP
american_scientific_journal-2511	39	27	94	94	NUM
american_scientific_journal-2511	39	28	c	c	NOUN
american_scientific_journal-2511	39	29	°	°	NUM
american_scientific_journal-2511	39	30	for	for	ADP
american_scientific_journal-2511	39	31	15	15	NUM
american_scientific_journal-2511	39	32	sec	sec	PROPN
american_scientific_journal-2511	39	33	.	.	PROPN
american_scientific_journal-2511	39	34	,	,	PUNCT
american_scientific_journal-2511	39	35	annealing	anneal	VERB
american_scientific_journal-2511	39	36	at	at	ADP
american_scientific_journal-2511	39	37	44	44	NUM
american_scientific_journal-2511	39	38	c	c	NOUN
american_scientific_journal-2511	39	39	°	°	NUM
american_scientific_journal-2511	39	40	for	for	ADP
american_scientific_journal-2511	39	41	20	20	NUM
american_scientific_journal-2511	39	42	sec	sec	NOUN
american_scientific_journal-2511	39	43	and	and	CCONJ
american_scientific_journal-2511	39	44	extension	extension	NOUN
american_scientific_journal-2511	39	45	at	at	ADP
american_scientific_journal-2511	39	46	70	70	NUM
american_scientific_journal-2511	39	47	c	c	NOUN
american_scientific_journal-2511	39	48	°	°	NUM
american_scientific_journal-2511	39	49	for	for	ADP
american_scientific_journal-2511	39	50	90	90	NUM
american_scientific_journal-2511	39	51	sec	sec	PROPN
american_scientific_journal-2511	39	52	.	.	PUNCT
american_scientific_journal-2511	40	1	with	with	ADP
american_scientific_journal-2511	40	2	final	final	ADJ
american_scientific_journal-2511	40	3	extension	extension	NOUN
american_scientific_journal-2511	40	4	72	72	NUM
american_scientific_journal-2511	40	5	c	c	NOUN
american_scientific_journal-2511	40	6	°	°	NOUN
american_scientific_journal-2511	40	7	7min	7min	NUM
american_scientific_journal-2511	40	8	.	.	PUNCT
american_scientific_journal-2511	41	1	[	[	X
american_scientific_journal-2511	41	2	1	1	NUM
american_scientific_journal-2511	41	3	]	]	PUNCT
american_scientific_journal-2511	41	4	.	.	PUNCT
american_scientific_journal-2511	42	1	2.3	2.3	NUM
american_scientific_journal-2511	42	2	purification	purification	NOUN
american_scientific_journal-2511	42	3	of	of	ADP
american_scientific_journal-2511	42	4	amplified	amplify	VERB
american_scientific_journal-2511	42	5	pcr	pcr	ADJ
american_scientific_journal-2511	42	6	products	product	NOUN
american_scientific_journal-2511	42	7	from	from	ADP
american_scientific_journal-2511	42	8	the	the	DET
american_scientific_journal-2511	42	9	agarose	agarose	NOUN
american_scientific_journal-2511	42	10	gel	gel	NOUN
american_scientific_journal-2511	42	11	the	the	DET
american_scientific_journal-2511	42	12	mega	mega	ADJ
american_scientific_journal-2511	42	13	quick	quick	ADJ
american_scientific_journal-2511	42	14	-	-	PUNCT
american_scientific_journal-2511	42	15	spintm	spintm	ADJ
american_scientific_journal-2511	42	16	purification	purification	NOUN
american_scientific_journal-2511	42	17	kit	kit	NOUN
american_scientific_journal-2511	42	18	(	(	PUNCT
american_scientific_journal-2511	42	19	intron	intron	PROPN
american_scientific_journal-2511	42	20	,	,	PUNCT
american_scientific_journal-2511	42	21	usa	usa	PROPN
american_scientific_journal-2511	42	22	)	)	PUNCT
american_scientific_journal-2511	42	23	catalog	catalog	NOUN
american_scientific_journal-2511	42	24	no	no	NOUN
american_scientific_journal-2511	42	25	.	.	NOUN
american_scientific_journal-2511	42	26	17286	17286	NUM
american_scientific_journal-2511	42	27	,	,	PUNCT
american_scientific_journal-2511	42	28	was	be	AUX
american_scientific_journal-2511	42	29	used	use	VERB
american_scientific_journal-2511	42	30	for	for	ADP
american_scientific_journal-2511	42	31	the	the	DET
american_scientific_journal-2511	42	32	purification	purification	NOUN
american_scientific_journal-2511	42	33	of	of	ADP
american_scientific_journal-2511	42	34	pcr	pcr	ADJ
american_scientific_journal-2511	42	35	product	product	NOUN
american_scientific_journal-2511	42	36	from	from	ADP
american_scientific_journal-2511	42	37	the	the	DET
american_scientific_journal-2511	42	38	gel	gel	NOUN
american_scientific_journal-2511	42	39	.	.	PUNCT
american_scientific_journal-2511	43	1	2.4	2.4	NUM
american_scientific_journal-2511	43	2	sequencing	sequencing	NOUN
american_scientific_journal-2511	43	3	of	of	ADP
american_scientific_journal-2511	43	4	the	the	DET
american_scientific_journal-2511	43	5	18s	18	NOUN
american_scientific_journal-2511	43	6	rdna	rdna	NOUN
american_scientific_journal-2511	43	7	and	and	CCONJ
american_scientific_journal-2511	43	8	co	co	NOUN
american_scientific_journal-2511	43	9	-	-	NOUN
american_scientific_journal-2511	43	10	i	i	PRON
american_scientific_journal-2511	43	11	genes	gene	VERB
american_scientific_journal-2511	43	12	the	the	DET
american_scientific_journal-2511	43	13	purified	purified	ADJ
american_scientific_journal-2511	43	14	18s	18	NOUN
american_scientific_journal-2511	43	15	rdna	rdna	PROPN
american_scientific_journal-2511	43	16	and	and	CCONJ
american_scientific_journal-2511	43	17	co	co	NOUN
american_scientific_journal-2511	43	18	-	-	NOUN
american_scientific_journal-2511	43	19	i	i	PRON
american_scientific_journal-2511	43	20	genes	gene	NOUN
american_scientific_journal-2511	43	21	sequencing	sequence	VERB
american_scientific_journal-2511	43	22	was	be	AUX
american_scientific_journal-2511	43	23	carried	carry	VERB
american_scientific_journal-2511	43	24	out	out	ADP
american_scientific_journal-2511	43	25	at	at	ADP
american_scientific_journal-2511	43	26	macrogen	macrogen	NOUN
american_scientific_journal-2511	43	27	company	company	NOUN
american_scientific_journal-2511	43	28	http://dna.macrogen.com	http://dna.macrogen.com	NOUN
american_scientific_journal-2511	43	29	.	.	PUNCT
american_scientific_journal-2511	44	1	2.5	2.5	NUM
american_scientific_journal-2511	44	2	rna	rna	NOUN
american_scientific_journal-2511	44	3	extraction	extraction	NOUN
american_scientific_journal-2511	44	4	total	total	NOUN
american_scientific_journal-2511	44	5	rna	rna	NOUN
american_scientific_journal-2511	44	6	from	from	ADP
american_scientific_journal-2511	44	7	medical	medical	ADJ
american_scientific_journal-2511	44	8	leeches	leech	NOUN
american_scientific_journal-2511	44	9	was	be	AUX
american_scientific_journal-2511	44	10	extracted	extract	VERB
american_scientific_journal-2511	44	11	using	use	VERB
american_scientific_journal-2511	44	12	rneasy	rneasy	NOUN
american_scientific_journal-2511	44	13	plus	plus	CCONJ
american_scientific_journal-2511	44	14	mini	mini	ADJ
american_scientific_journal-2511	44	15	kit	kit	NOUN
american_scientific_journal-2511	44	16	from	from	ADP
american_scientific_journal-2511	44	17	(	(	PUNCT
american_scientific_journal-2511	44	18	qiagen	qiagen	NOUN
american_scientific_journal-2511	44	19	company	company	NOUN
american_scientific_journal-2511	44	20	,	,	PUNCT
american_scientific_journal-2511	44	21	usa	usa	PROPN
american_scientific_journal-2511	44	22	)	)	PUNCT
american_scientific_journal-2511	44	23	catalog	catalog	NOUN
american_scientific_journal-2511	44	24	no	no	NOUN
american_scientific_journal-2511	44	25	.	.	PROPN
american_scientific_journal-2511	44	26	74134	74134	NUM
american_scientific_journal-2511	44	27	.	.	PUNCT
american_scientific_journal-2511	45	1	2.6	2.6	NUM
american_scientific_journal-2511	45	2	reverse	reverse	ADJ
american_scientific_journal-2511	45	3	transcription	transcription	NOUN
american_scientific_journal-2511	45	4	-	-	PUNCT
american_scientific_journal-2511	45	5	polymerase	polymerase	NOUN
american_scientific_journal-2511	45	6	chain	chain	NOUN
american_scientific_journal-2511	45	7	reaction	reaction	NOUN
american_scientific_journal-2511	45	8	(	(	PUNCT
american_scientific_journal-2511	45	9	rt	rt	NOUN
american_scientific_journal-2511	45	10	-	-	PUNCT
american_scientific_journal-2511	45	11	pcr	pcr	NOUN
american_scientific_journal-2511	45	12	)	)	PUNCT
american_scientific_journal-2511	45	13	the	the	DET
american_scientific_journal-2511	45	14	goscript	goscript	NOUN
american_scientific_journal-2511	45	15	™	™	VERB
american_scientific_journal-2511	45	16	reverse	reverse	VERB
american_scientific_journal-2511	45	17	transcription	transcription	NOUN
american_scientific_journal-2511	45	18	system	system	NOUN
american_scientific_journal-2511	45	19	from	from	ADP
american_scientific_journal-2511	45	20	(	(	PUNCT
american_scientific_journal-2511	45	21	promega	promega	PROPN
american_scientific_journal-2511	45	22	,	,	PUNCT
american_scientific_journal-2511	45	23	usa	usa	PROPN
american_scientific_journal-2511	45	24	)	)	PUNCT
american_scientific_journal-2511	45	25	catalog	catalog	NOUN
american_scientific_journal-2511	45	26	no	no	NOUN
american_scientific_journal-2511	45	27	.	.	PUNCT
american_scientific_journal-2511	46	1	a5000	a5000	PROPN
american_scientific_journal-2511	46	2	was	be	AUX
american_scientific_journal-2511	46	3	used	use	VERB
american_scientific_journal-2511	46	4	for	for	ADP
american_scientific_journal-2511	46	5	the	the	DET
american_scientific_journal-2511	46	6	synthesis	synthesis	NOUN
american_scientific_journal-2511	46	7	of	of	ADP
american_scientific_journal-2511	46	8	cdna	cdna	PROPN
american_scientific_journal-2511	46	9	(	(	PUNCT
american_scientific_journal-2511	46	10	to	to	PART
american_scientific_journal-2511	46	11	amplify	amplify	VERB
american_scientific_journal-2511	46	12	hirudin	hirudin	VERB
american_scientific_journal-2511	46	13	gene	gene	NOUN
american_scientific_journal-2511	46	14	)	)	PUNCT
american_scientific_journal-2511	46	15	.	.	PUNCT
american_scientific_journal-2511	47	1	2.7	2.7	NUM
american_scientific_journal-2511	47	2	amplification	amplification	NOUN
american_scientific_journal-2511	47	3	of	of	ADP
american_scientific_journal-2511	47	4	hirudin	hirudin	VERB
american_scientific_journal-2511	47	5	gene	gene	NOUN
american_scientific_journal-2511	47	6	hr1	hr1	NOUN
american_scientific_journal-2511	47	7	(	(	PUNCT
american_scientific_journal-2511	47	8	5´-agtgcatattgggttctaatgga-3	5´-agtgcatattgggttctaatgga-3	NUM
american_scientific_journal-2511	47	9	´	´	NOUN
american_scientific_journal-2511	47	10	)	)	PUNCT
american_scientific_journal-2511	47	11	and	and	CCONJ
american_scientific_journal-2511	47	12	hr2	hr2	PROPN
american_scientific_journal-2511	47	13	(	(	PUNCT
american_scientific_journal-2511	47	14	5´-tggtaaatagctgaatatga	5´-tggtaaatagctgaatatga	NUM
american_scientific_journal-2511	47	15	ttgagag-3	ttgagag-3	NUM
american_scientific_journal-2511	47	16	´	´	NOUN
american_scientific_journal-2511	47	17	)	)	PUNCT
american_scientific_journal-2511	47	18	primers	primer	NOUN
american_scientific_journal-2511	47	19	were	be	AUX
american_scientific_journal-2511	47	20	designed	design	VERB
american_scientific_journal-2511	47	21	to	to	PART
american_scientific_journal-2511	47	22	amplify	amplify	VERB
american_scientific_journal-2511	47	23	hirudin	hirudin	VERB
american_scientific_journal-2511	47	24	gene	gene	NOUN
american_scientific_journal-2511	47	25	named	name	VERB
american_scientific_journal-2511	47	26	as	as	ADP
american_scientific_journal-2511	47	27	hr	hr	NOUN
american_scientific_journal-2511	47	28	.	.	PUNCT
american_scientific_journal-2511	48	1	gene	gene	NOUN
american_scientific_journal-2511	48	2	amplification	amplification	NOUN
american_scientific_journal-2511	48	3	was	be	AUX
american_scientific_journal-2511	48	4	american	american	ADJ
american_scientific_journal-2511	48	5	scientific	scientific	ADJ
american_scientific_journal-2511	48	6	research	research	NOUN
american_scientific_journal-2511	48	7	journal	journal	NOUN
american_scientific_journal-2511	48	8	for	for	ADP
american_scientific_journal-2511	48	9	engineering	engineering	NOUN
american_scientific_journal-2511	48	10	,	,	PUNCT
american_scientific_journal-2511	48	11	technology	technology	NOUN
american_scientific_journal-2511	48	12	,	,	PUNCT
american_scientific_journal-2511	48	13	and	and	CCONJ
american_scientific_journal-2511	48	14	sciences	science	NOUN
american_scientific_journal-2511	48	15	(	(	PUNCT
american_scientific_journal-2511	48	16	asrjets	asrjet	NOUN
american_scientific_journal-2511	48	17	)	)	PUNCT
american_scientific_journal-2511	48	18	(	(	PUNCT
american_scientific_journal-2511	48	19	2017	2017	NUM
american_scientific_journal-2511	48	20	)	)	PUNCT
american_scientific_journal-2511	48	21	volume	volume	NOUN
american_scientific_journal-2511	48	22	27	27	NUM
american_scientific_journal-2511	48	23	,	,	PUNCT
american_scientific_journal-2511	48	24	no	no	DET
american_scientific_journal-2511	48	25	1	1	NUM
american_scientific_journal-2511	48	26	,	,	PUNCT
american_scientific_journal-2511	48	27	pp	pp	ADV
american_scientific_journal-2511	48	28	25	25	NUM
american_scientific_journal-2511	48	29	-	-	SYM
american_scientific_journal-2511	48	30	42	42	NUM
american_scientific_journal-2511	48	31	28	28	NUM
american_scientific_journal-2511	48	32	performed	perform	VERB
american_scientific_journal-2511	48	33	in	in	ADP
american_scientific_journal-2511	48	34	a	a	DET
american_scientific_journal-2511	48	35	final	final	ADJ
american_scientific_journal-2511	48	36	volume	volume	NOUN
american_scientific_journal-2511	48	37	of	of	ADP
american_scientific_journal-2511	48	38	25µl	25µl	NOUN
american_scientific_journal-2511	48	39	of	of	ADP
american_scientific_journal-2511	48	40	reaction	reaction	NOUN
american_scientific_journal-2511	48	41	mixtures	mixture	NOUN
american_scientific_journal-2511	48	42	contained	contain	VERB
american_scientific_journal-2511	48	43	5µl	5µl	ADJ
american_scientific_journal-2511	48	44	cdna	cdna	PROPN
american_scientific_journal-2511	48	45	,	,	PUNCT
american_scientific_journal-2511	48	46	1µl	1µl	PROPN
american_scientific_journal-2511	48	47	hr1	hr1	PROPN
american_scientific_journal-2511	48	48	primer	primer	NOUN
american_scientific_journal-2511	48	49	,	,	PUNCT
american_scientific_journal-2511	48	50	1µ	1µ	NUM
american_scientific_journal-2511	48	51	hr2	hr2	PROPN
american_scientific_journal-2511	48	52	primer	primer	NOUN
american_scientific_journal-2511	48	53	,	,	PUNCT
american_scientific_journal-2511	48	54	12.5µl	12.5µl	NUM
american_scientific_journal-2511	48	55	pcr	pcr	NOUN
american_scientific_journal-2511	48	56	master	master	NOUN
american_scientific_journal-2511	48	57	mix	mix	NOUN
american_scientific_journal-2511	48	58	promega	promega	PROPN
american_scientific_journal-2511	48	59	and	and	CCONJ
american_scientific_journal-2511	48	60	5.5µl	5.5µl	NUM
american_scientific_journal-2511	48	61	nuclease	nuclease	NOUN
american_scientific_journal-2511	48	62	-	-	PUNCT
american_scientific_journal-2511	48	63	free	free	ADJ
american_scientific_journal-2511	48	64	water	water	NOUN
american_scientific_journal-2511	48	65	.	.	PUNCT
american_scientific_journal-2511	49	1	pcr	pcr	ADJ
american_scientific_journal-2511	49	2	condition	condition	NOUN
american_scientific_journal-2511	49	3	for	for	ADP
american_scientific_journal-2511	49	4	amplification	amplification	NOUN
american_scientific_journal-2511	49	5	of	of	ADP
american_scientific_journal-2511	49	6	hirudin	hirudin	VERB
american_scientific_journal-2511	49	7	gene	gene	NOUN
american_scientific_journal-2511	49	8	were	be	AUX
american_scientific_journal-2511	49	9	:	:	PUNCT
american_scientific_journal-2511	49	10	initial	initial	ADJ
american_scientific_journal-2511	49	11	denaturation	denaturation	NOUN
american_scientific_journal-2511	49	12	at	at	ADP
american_scientific_journal-2511	49	13	95	95	NUM
american_scientific_journal-2511	49	14	c	c	NOUN
american_scientific_journal-2511	49	15	°	°	NUM
american_scientific_journal-2511	49	16	for	for	ADP
american_scientific_journal-2511	49	17	1min	1min	NUM
american_scientific_journal-2511	49	18	.	.	PUNCT
american_scientific_journal-2511	49	19	followed	follow	VERB
american_scientific_journal-2511	49	20	by	by	ADP
american_scientific_journal-2511	49	21	30	30	NUM
american_scientific_journal-2511	49	22	cycle	cycle	NOUN
american_scientific_journal-2511	49	23	of	of	ADP
american_scientific_journal-2511	49	24	denaturation	denaturation	NOUN
american_scientific_journal-2511	49	25	at	at	ADP
american_scientific_journal-2511	49	26	95	95	NUM
american_scientific_journal-2511	49	27	c	c	NOUN
american_scientific_journal-2511	49	28	°	°	NUM
american_scientific_journal-2511	49	29	30	30	NUM
american_scientific_journal-2511	49	30	sec	sec	PROPN
american_scientific_journal-2511	49	31	.	.	PROPN
american_scientific_journal-2511	49	32	,	,	PUNCT
american_scientific_journal-2511	49	33	annealing	anneal	VERB
american_scientific_journal-2511	49	34	at	at	ADP
american_scientific_journal-2511	49	35	63.5	63.5	NUM
american_scientific_journal-2511	49	36	c	c	NOUN
american_scientific_journal-2511	49	37	°	°	NUM
american_scientific_journal-2511	49	38	30	30	NUM
american_scientific_journal-2511	49	39	sec	sec	PROPN
american_scientific_journal-2511	49	40	.	.	PROPN
american_scientific_journal-2511	50	1	and	and	CCONJ
american_scientific_journal-2511	50	2	extension	extension	NOUN
american_scientific_journal-2511	50	3	at	at	ADP
american_scientific_journal-2511	50	4	72	72	NUM
american_scientific_journal-2511	50	5	c	c	NOUN
american_scientific_journal-2511	50	6	°	°	NUM
american_scientific_journal-2511	50	7	30	30	NUM
american_scientific_journal-2511	50	8	sec	sec	PROPN
american_scientific_journal-2511	50	9	.	.	PROPN
american_scientific_journal-2511	51	1	with	with	ADP
american_scientific_journal-2511	51	2	final	final	ADJ
american_scientific_journal-2511	51	3	extension	extension	NOUN
american_scientific_journal-2511	51	4	at	at	ADP
american_scientific_journal-2511	51	5	72	72	NUM
american_scientific_journal-2511	51	6	c	c	NOUN
american_scientific_journal-2511	51	7	°	°	NUM
american_scientific_journal-2511	51	8	for	for	ADP
american_scientific_journal-2511	51	9	7	7	NUM
american_scientific_journal-2511	51	10	min	min	NOUN
american_scientific_journal-2511	51	11	.	.	PUNCT
american_scientific_journal-2511	52	1	the	the	DET
american_scientific_journal-2511	52	2	product	product	NOUN
american_scientific_journal-2511	52	3	was	be	AUX
american_scientific_journal-2511	52	4	also	also	ADV
american_scientific_journal-2511	52	5	purified	purify	VERB
american_scientific_journal-2511	52	6	from	from	ADP
american_scientific_journal-2511	52	7	the	the	DET
american_scientific_journal-2511	52	8	gel	gel	NOUN
american_scientific_journal-2511	52	9	.	.	PUNCT
american_scientific_journal-2511	53	1	2.8	2.8	NUM
american_scientific_journal-2511	53	2	cloning	cloning	NOUN
american_scientific_journal-2511	53	3	project	project	NOUN
american_scientific_journal-2511	53	4	the	the	DET
american_scientific_journal-2511	53	5	cloning	cloning	NOUN
american_scientific_journal-2511	53	6	technique	technique	NOUN
american_scientific_journal-2511	53	7	was	be	AUX
american_scientific_journal-2511	53	8	done	do	VERB
american_scientific_journal-2511	53	9	between	between	ADP
american_scientific_journal-2511	53	10	amplified	amplify	VERB
american_scientific_journal-2511	53	11	product	product	NOUN
american_scientific_journal-2511	53	12	by	by	ADP
american_scientific_journal-2511	53	13	(	(	PUNCT
american_scientific_journal-2511	53	14	promega	promega	PROPN
american_scientific_journal-2511	53	15	master	master	PROPN
american_scientific_journal-2511	53	16	mix	mix	PROPN
american_scientific_journal-2511	53	17	)	)	PUNCT
american_scientific_journal-2511	53	18	to	to	PART
american_scientific_journal-2511	53	19	provide	provide	VERB
american_scientific_journal-2511	53	20	3ʼa	3ʼa	ADJ
american_scientific_journal-2511	53	21	-	-	PUNCT
american_scientific_journal-2511	53	22	overhang	overhang	NOUN
american_scientific_journal-2511	53	23	,	,	PUNCT
american_scientific_journal-2511	53	24	with	with	ADP
american_scientific_journal-2511	53	25	pet-16b	pet-16b	NUM
american_scientific_journal-2511	53	26	(	(	PUNCT
american_scientific_journal-2511	53	27	novagen	novagen	NOUN
american_scientific_journal-2511	53	28	company	company	NOUN
american_scientific_journal-2511	53	29	,	,	PUNCT
american_scientific_journal-2511	53	30	usa	usa	PROPN
american_scientific_journal-2511	53	31	)	)	PUNCT
american_scientific_journal-2511	53	32	catalog	catalog	NOUN
american_scientific_journal-2511	53	33	no	no	NOUN
american_scientific_journal-2511	53	34	.	.	PROPN
american_scientific_journal-2511	53	35	69662	69662	NUM
american_scientific_journal-2511	53	36	-	-	SYM
american_scientific_journal-2511	53	37	3	3	NUM
american_scientific_journal-2511	53	38	.	.	NUM
american_scientific_journal-2511	53	39	which	which	PRON
american_scientific_journal-2511	53	40	restricted	restrict	VERB
american_scientific_journal-2511	53	41	with	with	ADP
american_scientific_journal-2511	53	42	bamhi	bamhi	NOUN
american_scientific_journal-2511	53	43	restriction	restriction	NOUN
american_scientific_journal-2511	53	44	enzyme	enzyme	NOUN
american_scientific_journal-2511	53	45	(	(	PUNCT
american_scientific_journal-2511	53	46	promega	promega	PROPN
american_scientific_journal-2511	53	47	,	,	PUNCT
american_scientific_journal-2511	53	48	usa	usa	PROPN
american_scientific_journal-2511	53	49	)	)	PUNCT
american_scientific_journal-2511	53	50	and	and	CCONJ
american_scientific_journal-2511	53	51	ligation	ligation	NOUN
american_scientific_journal-2511	53	52	kit	kit	NOUN
american_scientific_journal-2511	53	53	from	from	ADP
american_scientific_journal-2511	53	54	(	(	PUNCT
american_scientific_journal-2511	53	55	bioneer	bioneer	NOUN
american_scientific_journal-2511	53	56	,	,	PUNCT
american_scientific_journal-2511	53	57	korea	korea	PROPN
american_scientific_journal-2511	53	58	)	)	PUNCT
american_scientific_journal-2511	53	59	catalog	catalog	NOUN
american_scientific_journal-2511	53	60	no	no	INTJ
american_scientific_journal-2511	53	61	.	.	PUNCT
american_scientific_journal-2511	54	1	k-7103	k-7103	AUX
american_scientific_journal-2511	54	2	.	.	NOUN
american_scientific_journal-2511	54	3	2.9	2.9	NUM
american_scientific_journal-2511	54	4	transformation	transformation	NOUN
american_scientific_journal-2511	54	5	process	process	NOUN
american_scientific_journal-2511	54	6	transformation	transformation	NOUN
american_scientific_journal-2511	54	7	process	process	NOUN
american_scientific_journal-2511	54	8	has	have	AUX
american_scientific_journal-2511	54	9	been	be	AUX
american_scientific_journal-2511	54	10	done	do	VERB
american_scientific_journal-2511	54	11	by	by	ADP
american_scientific_journal-2511	54	12	using	use	VERB
american_scientific_journal-2511	54	13	dh5α	dh5α	PROPN
american_scientific_journal-2511	54	14	tm	tm	DET
american_scientific_journal-2511	54	15	competent	competent	ADJ
american_scientific_journal-2511	54	16	cells	cell	NOUN
american_scientific_journal-2511	54	17	(	(	PUNCT
american_scientific_journal-2511	54	18	invitrogen	invitrogen	PROPN
american_scientific_journal-2511	54	19	,	,	PUNCT
american_scientific_journal-2511	54	20	usa	usa	PROPN
american_scientific_journal-2511	54	21	)	)	PUNCT
american_scientific_journal-2511	54	22	catalog	catalog	NOUN
american_scientific_journal-2511	54	23	no	no	NOUN
american_scientific_journal-2511	54	24	.	.	PROPN
american_scientific_journal-2511	54	25	18258012	18258012	NUM
american_scientific_journal-2511	54	26	and	and	CCONJ
american_scientific_journal-2511	54	27	according	accord	VERB
american_scientific_journal-2511	54	28	the	the	DET
american_scientific_journal-2511	54	29	company	company	NOUN
american_scientific_journal-2511	54	30	protocol	protocol	NOUN
american_scientific_journal-2511	54	31	and	and	CCONJ
american_scientific_journal-2511	54	32	cultured	cultured	ADJ
american_scientific_journal-2511	54	33	on	on	ADP
american_scientific_journal-2511	54	34	gal	gal	PROPN
american_scientific_journal-2511	54	35	/	/	SYM
american_scientific_journal-2511	54	36	iptg	iptg	NOUN
american_scientific_journal-2511	54	37	lb	lb	ADP
american_scientific_journal-2511	54	38	agar	agar	NOUN
american_scientific_journal-2511	54	39	plates	plate	NOUN
american_scientific_journal-2511	54	40	then	then	ADV
american_scientific_journal-2511	54	41	plasmid	plasmid	NOUN
american_scientific_journal-2511	54	42	was	be	AUX
american_scientific_journal-2511	54	43	extracted	extract	VERB
american_scientific_journal-2511	54	44	from	from	ADP
american_scientific_journal-2511	54	45	dh5α	dh5α	PROPN
american_scientific_journal-2511	54	46	by	by	ADP
american_scientific_journal-2511	54	47	using	use	VERB
american_scientific_journal-2511	54	48	accuprep	accuprep	NOUN
american_scientific_journal-2511	54	49	®	®	NOUN
american_scientific_journal-2511	54	50	plasmid	plasmid	NOUN
american_scientific_journal-2511	54	51	mini	mini	ADJ
american_scientific_journal-2511	54	52	extraction	extraction	NOUN
american_scientific_journal-2511	54	53	kit	kit	NOUN
american_scientific_journal-2511	54	54	(	(	PUNCT
american_scientific_journal-2511	54	55	bioneer	bioneer	NOUN
american_scientific_journal-2511	54	56	,	,	PUNCT
american_scientific_journal-2511	54	57	korea	korea	PROPN
american_scientific_journal-2511	54	58	)	)	PUNCT
american_scientific_journal-2511	55	1	catalog	catalog	NOUN
american_scientific_journal-2511	55	2	no	no	INTJ
american_scientific_journal-2511	55	3	.	.	PUNCT
american_scientific_journal-2511	56	1	k-3030	k-3030	PROPN
american_scientific_journal-2511	57	1	and	and	CCONJ
american_scientific_journal-2511	58	1	inserted	insert	VERB
american_scientific_journal-2511	58	2	it	it	PRON
american_scientific_journal-2511	58	3	into	into	ADP
american_scientific_journal-2511	58	4	bl21(de3	bl21(de3	VERB
american_scientific_journal-2511	58	5	)	)	PUNCT
american_scientific_journal-2511	58	6	strain	strain	NOUN
american_scientific_journal-2511	58	7	(	(	PUNCT
american_scientific_journal-2511	58	8	novagen	novagen	PROPN
american_scientific_journal-2511	58	9	,	,	PUNCT
american_scientific_journal-2511	58	10	usa	usa	PROPN
american_scientific_journal-2511	58	11	)	)	PUNCT
american_scientific_journal-2511	58	12	catalog	catalog	NOUN
american_scientific_journal-2511	58	13	no	no	NOUN
american_scientific_journal-2511	58	14	.	.	PUNCT
american_scientific_journal-2511	58	15	69450	69450	NUM
american_scientific_journal-2511	58	16	,	,	PUNCT
american_scientific_journal-2511	58	17	for	for	ADP
american_scientific_journal-2511	58	18	protein	protein	NOUN
american_scientific_journal-2511	58	19	expression	expression	NOUN
american_scientific_journal-2511	58	20	.	.	PUNCT
american_scientific_journal-2511	59	1	then	then	ADV
american_scientific_journal-2511	59	2	,	,	PUNCT
american_scientific_journal-2511	59	3	bl21(de3	bl21(de3	ADJ
american_scientific_journal-2511	59	4	)	)	PUNCT
american_scientific_journal-2511	59	5	cells	cell	NOUN
american_scientific_journal-2511	59	6	cultured	cultured	ADJ
american_scientific_journal-2511	59	7	on	on	ADP
american_scientific_journal-2511	59	8	lb	lb	NUM
american_scientific_journal-2511	59	9	agar	agar	NOUN
american_scientific_journal-2511	59	10	medium	medium	NOUN
american_scientific_journal-2511	59	11	.	.	PUNCT
american_scientific_journal-2511	60	1	plasmid	plasmid	NOUN
american_scientific_journal-2511	60	2	was	be	AUX
american_scientific_journal-2511	60	3	extracted	extract	VERB
american_scientific_journal-2511	60	4	from	from	ADP
american_scientific_journal-2511	60	5	transformed	transform	VERB
american_scientific_journal-2511	60	6	cells	cell	NOUN
american_scientific_journal-2511	60	7	and	and	CCONJ
american_scientific_journal-2511	60	8	analyzed	analyze	VERB
american_scientific_journal-2511	60	9	by	by	ADP
american_scientific_journal-2511	60	10	2	2	NUM
american_scientific_journal-2511	60	11	%	%	NOUN
american_scientific_journal-2511	60	12	agarose	agarose	NOUN
american_scientific_journal-2511	60	13	gel	gel	NOUN
american_scientific_journal-2511	60	14	containing	contain	VERB
american_scientific_journal-2511	60	15	1	1	NUM
american_scientific_journal-2511	60	16	%	%	NOUN
american_scientific_journal-2511	60	17	ethidium	ethidium	NOUN
american_scientific_journal-2511	60	18	bromide	bromide	NOUN
american_scientific_journal-2511	60	19	,	,	PUNCT
american_scientific_journal-2511	60	20	also	also	ADV
american_scientific_journal-2511	60	21	,	,	PUNCT
american_scientific_journal-2511	60	22	hirudin	hirudin	VERB
american_scientific_journal-2511	60	23	gene	gene	NOUN
american_scientific_journal-2511	60	24	was	be	AUX
american_scientific_journal-2511	60	25	amplified	amplify	VERB
american_scientific_journal-2511	60	26	by	by	ADP
american_scientific_journal-2511	60	27	hr1&hr2	hr1&hr2	NOUN
american_scientific_journal-2511	60	28	and	and	CCONJ
american_scientific_journal-2511	60	29	analyzed	analyze	VERB
american_scientific_journal-2511	60	30	by	by	ADP
american_scientific_journal-2511	60	31	2	2	NUM
american_scientific_journal-2511	60	32	%	%	NOUN
american_scientific_journal-2511	60	33	agarose	agarose	X
american_scientific_journal-2511	60	34	gel	gel	NOUN
american_scientific_journal-2511	60	35	.	.	PUNCT
american_scientific_journal-2511	61	1	2.10	2.10	NUM
american_scientific_journal-2511	61	2	hirudin	hirudin	NOUN
american_scientific_journal-2511	61	3	expression	expression	NOUN
american_scientific_journal-2511	61	4	in	in	ADP
american_scientific_journal-2511	61	5	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2511	61	6	)	)	PUNCT
american_scientific_journal-2511	61	7	the	the	DET
american_scientific_journal-2511	61	8	protein	protein	NOUN
american_scientific_journal-2511	61	9	expression	expression	NOUN
american_scientific_journal-2511	61	10	and	and	CCONJ
american_scientific_journal-2511	61	11	production	production	NOUN
american_scientific_journal-2511	61	12	in	in	ADP
american_scientific_journal-2511	61	13	bacteria	bacteria	NOUN
american_scientific_journal-2511	61	14	was	be	AUX
american_scientific_journal-2511	61	15	carried	carry	VERB
american_scientific_journal-2511	61	16	out	out	ADP
american_scientific_journal-2511	61	17	according	accord	VERB
american_scientific_journal-2511	61	18	to	to	ADP
american_scientific_journal-2511	61	19	novagen	novagen	NOUN
american_scientific_journal-2511	61	20	[	[	X
american_scientific_journal-2511	61	21	9	9	NUM
american_scientific_journal-2511	61	22	]	]	X
american_scientific_journal-2511	61	23	protocol	protocol	NOUN
american_scientific_journal-2511	61	24	and	and	CCONJ
american_scientific_journal-2511	61	25	the	the	DET
american_scientific_journal-2511	61	26	fermentation	fermentation	NOUN
american_scientific_journal-2511	61	27	media	medium	NOUN
american_scientific_journal-2511	61	28	:	:	PUNCT
american_scientific_journal-2511	61	29	lb	lb	DET
american_scientific_journal-2511	61	30	broth	broth	ADJ
american_scientific_journal-2511	61	31	medium	medium	NOUN
american_scientific_journal-2511	61	32	was	be	AUX
american_scientific_journal-2511	61	33	also	also	ADV
american_scientific_journal-2511	61	34	prepared	prepare	VERB
american_scientific_journal-2511	61	35	according	accord	VERB
american_scientific_journal-2511	61	36	to	to	ADP
american_scientific_journal-2511	61	37	novagen	novagen	NOUN
american_scientific_journal-2511	61	38	[	[	X
american_scientific_journal-2511	61	39	9	9	NUM
american_scientific_journal-2511	61	40	]	]	PUNCT
american_scientific_journal-2511	61	41	.	.	PUNCT
american_scientific_journal-2511	62	1	2.11	2.11	NUM
american_scientific_journal-2511	62	2	fast	fast	ADJ
american_scientific_journal-2511	62	3	screening	screening	NOUN
american_scientific_journal-2511	62	4	for	for	ADP
american_scientific_journal-2511	62	5	hr	hr	NOUN
american_scientific_journal-2511	62	6	gene	gene	NOUN
american_scientific_journal-2511	62	7	by	by	ADP
american_scientific_journal-2511	62	8	digestion	digestion	NOUN
american_scientific_journal-2511	62	9	with	with	ADP
american_scientific_journal-2511	62	10	restriction	restriction	NOUN
american_scientific_journal-2511	62	11	enzyme	enzyme	NOUN
american_scientific_journal-2511	62	12	to	to	PART
american_scientific_journal-2511	62	13	determine	determine	VERB
american_scientific_journal-2511	62	14	the	the	DET
american_scientific_journal-2511	62	15	presence	presence	NOUN
american_scientific_journal-2511	62	16	of	of	ADP
american_scientific_journal-2511	62	17	hr	hr	NOUN
american_scientific_journal-2511	62	18	gene	gene	NOUN
american_scientific_journal-2511	62	19	which	which	PRON
american_scientific_journal-2511	62	20	constructed	construct	VERB
american_scientific_journal-2511	62	21	in	in	ADP
american_scientific_journal-2511	62	22	pet-16b	pet-16b	NUM
american_scientific_journal-2511	62	23	vector	vector	NOUN
american_scientific_journal-2511	62	24	,	,	PUNCT
american_scientific_journal-2511	62	25	constructive	constructive	ADJ
american_scientific_journal-2511	62	26	vector	vector	NOUN
american_scientific_journal-2511	62	27	was	be	AUX
american_scientific_journal-2511	62	28	digested	digest	VERB
american_scientific_journal-2511	62	29	with	with	ADP
american_scientific_journal-2511	62	30	two	two	NUM
american_scientific_journal-2511	62	31	restriction	restriction	NOUN
american_scientific_journal-2511	62	32	enzyme	enzyme	NOUN
american_scientific_journal-2511	62	33	hindiii	hindiii	NOUN
american_scientific_journal-2511	62	34	(	(	PUNCT
american_scientific_journal-2511	62	35	bioneer	bioneer	NOUN
american_scientific_journal-2511	62	36	,	,	PUNCT
american_scientific_journal-2511	62	37	korea	korea	PROPN
american_scientific_journal-2511	62	38	)	)	PUNCT
american_scientific_journal-2511	62	39	,	,	PUNCT
american_scientific_journal-2511	62	40	nde1(biolab	nde1(biolab	NOUN
american_scientific_journal-2511	62	41	,	,	PUNCT
american_scientific_journal-2511	62	42	u.k	u.k	PROPN
american_scientific_journal-2511	62	43	.	.	PROPN
american_scientific_journal-2511	62	44	)	)	PUNCT
american_scientific_journal-2511	62	45	.	.	PUNCT
american_scientific_journal-2511	63	1	also	also	ADV
american_scientific_journal-2511	63	2	,	,	PUNCT
american_scientific_journal-2511	63	3	this	this	DET
american_scientific_journal-2511	63	4	procedure	procedure	NOUN
american_scientific_journal-2511	63	5	repeated	repeat	VERB
american_scientific_journal-2511	63	6	with	with	ADP
american_scientific_journal-2511	63	7	control	control	NOUN
american_scientific_journal-2511	63	8	vector	vector	NOUN
american_scientific_journal-2511	63	9	(	(	PUNCT
american_scientific_journal-2511	63	10	without	without	ADP
american_scientific_journal-2511	63	11	inserted	insert	VERB
american_scientific_journal-2511	63	12	hirudin	hirudin	NOUN
american_scientific_journal-2511	63	13	)	)	PUNCT
american_scientific_journal-2511	63	14	.	.	PUNCT
american_scientific_journal-2511	64	1	2.12	2.12	NUM
american_scientific_journal-2511	64	2	fast	fast	ADJ
american_scientific_journal-2511	64	3	screening	screening	NOUN
american_scientific_journal-2511	64	4	for	for	ADP
american_scientific_journal-2511	64	5	hr	hr	NOUN
american_scientific_journal-2511	64	6	gene	gene	NOUN
american_scientific_journal-2511	64	7	by	by	ADP
american_scientific_journal-2511	64	8	digestion	digestion	NOUN
american_scientific_journal-2511	64	9	with	with	ADP
american_scientific_journal-2511	64	10	restriction	restriction	NOUN
american_scientific_journal-2511	64	11	enzyme	enzyme	NOUN
american_scientific_journal-2511	64	12	to	to	PART
american_scientific_journal-2511	64	13	determine	determine	VERB
american_scientific_journal-2511	64	14	the	the	DET
american_scientific_journal-2511	64	15	presence	presence	NOUN
american_scientific_journal-2511	64	16	of	of	ADP
american_scientific_journal-2511	64	17	hr	hr	NOUN
american_scientific_journal-2511	64	18	gene	gene	NOUN
american_scientific_journal-2511	64	19	which	which	PRON
american_scientific_journal-2511	64	20	constructed	construct	VERB
american_scientific_journal-2511	64	21	in	in	ADP
american_scientific_journal-2511	64	22	pet-16b	pet-16b	NUM
american_scientific_journal-2511	64	23	vector	vector	NOUN
american_scientific_journal-2511	64	24	,	,	PUNCT
american_scientific_journal-2511	64	25	constructive	constructive	ADJ
american_scientific_journal-2511	64	26	vector	vector	NOUN
american_scientific_journal-2511	64	27	was	be	AUX
american_scientific_journal-2511	64	28	digested	digest	VERB
american_scientific_journal-2511	64	29	with	with	ADP
american_scientific_journal-2511	64	30	two	two	NUM
american_scientific_journal-2511	64	31	restriction	restriction	NOUN
american_scientific_journal-2511	64	32	enzyme	enzyme	NOUN
american_scientific_journal-2511	64	33	hindiii	hindiii	NOUN
american_scientific_journal-2511	64	34	(	(	PUNCT
american_scientific_journal-2511	64	35	bioneer	bioneer	NOUN
american_scientific_journal-2511	64	36	,	,	PUNCT
american_scientific_journal-2511	64	37	korea	korea	PROPN
american_scientific_journal-2511	64	38	)	)	PUNCT
american_scientific_journal-2511	64	39	,	,	PUNCT
american_scientific_journal-2511	64	40	nde1	nde1	PROPN
american_scientific_journal-2511	64	41	(	(	PUNCT
american_scientific_journal-2511	64	42	biolab	biolab	PROPN
american_scientific_journal-2511	64	43	,	,	PUNCT
american_scientific_journal-2511	64	44	u.k	u.k	PROPN
american_scientific_journal-2511	64	45	.	.	PROPN
american_scientific_journal-2511	64	46	)	)	PUNCT
american_scientific_journal-2511	64	47	.	.	PUNCT
american_scientific_journal-2511	65	1	also	also	ADV
american_scientific_journal-2511	65	2	,	,	PUNCT
american_scientific_journal-2511	65	3	this	this	DET
american_scientific_journal-2511	65	4	procedure	procedure	NOUN
american_scientific_journal-2511	65	5	repeated	repeat	VERB
american_scientific_journal-2511	65	6	with	with	ADP
american_scientific_journal-2511	65	7	control	control	NOUN
american_scientific_journal-2511	65	8	vector	vector	NOUN
american_scientific_journal-2511	65	9	(	(	PUNCT
american_scientific_journal-2511	65	10	without	without	ADP
american_scientific_journal-2511	65	11	inserted	insert	VERB
american_scientific_journal-2511	65	12	hirudin	hirudin	NOUN
american_scientific_journal-2511	65	13	)	)	PUNCT
american_scientific_journal-2511	65	14	.	.	PUNCT
american_scientific_journal-2511	66	1	2.13	2.13	NUM
american_scientific_journal-2511	66	2	detection	detection	NOUN
american_scientific_journal-2511	66	3	of	of	ADP
american_scientific_journal-2511	66	4	hirudin	hirudin	VERB
american_scientific_journal-2511	66	5	expression	expression	NOUN
american_scientific_journal-2511	66	6	by	by	ADP
american_scientific_journal-2511	66	7	real	real	ADJ
american_scientific_journal-2511	66	8	time	time	NOUN
american_scientific_journal-2511	66	9	pcr	pcr	PROPN
american_scientific_journal-2511	66	10	this	this	DET
american_scientific_journal-2511	66	11	process	process	NOUN
american_scientific_journal-2511	66	12	has	have	AUX
american_scientific_journal-2511	66	13	been	be	AUX
american_scientific_journal-2511	66	14	done	do	VERB
american_scientific_journal-2511	66	15	by	by	ADP
american_scientific_journal-2511	66	16	extraction	extraction	NOUN
american_scientific_journal-2511	66	17	of	of	ADP
american_scientific_journal-2511	66	18	total	total	ADJ
american_scientific_journal-2511	66	19	rna	rna	NOUN
american_scientific_journal-2511	66	20	from	from	ADP
american_scientific_journal-2511	66	21	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2511	66	22	)	)	PUNCT
american_scientific_journal-2511	66	23	strain	strain	NOUN
american_scientific_journal-2511	66	24	from	from	ADP
american_scientific_journal-2511	66	25	fermentation	fermentation	NOUN
american_scientific_journal-2511	66	26	medium	medium	NOUN
american_scientific_journal-2511	66	27	by	by	ADP
american_scientific_journal-2511	66	28	using	use	VERB
american_scientific_journal-2511	66	29	accuzoltm	accuzoltm	NOUN
american_scientific_journal-2511	66	30	reagent	reagent	NOUN
american_scientific_journal-2511	66	31	(	(	PUNCT
american_scientific_journal-2511	66	32	bioneer	bioneer	NOUN
american_scientific_journal-2511	66	33	,	,	PUNCT
american_scientific_journal-2511	66	34	korea	korea	PROPN
american_scientific_journal-2511	66	35	)	)	PUNCT
american_scientific_journal-2511	66	36	,	,	PUNCT
american_scientific_journal-2511	66	37	the	the	DET
american_scientific_journal-2511	66	38	concentration	concentration	NOUN
american_scientific_journal-2511	66	39	of	of	ADP
american_scientific_journal-2511	66	40	extracted	extract	VERB
american_scientific_journal-2511	66	41	rna	rna	NOUN
american_scientific_journal-2511	66	42	was	be	AUX
american_scientific_journal-2511	66	43	determined	determine	VERB
american_scientific_journal-2511	66	44	according	accord	VERB
american_scientific_journal-2511	66	45	to	to	ADP
american_scientific_journal-2511	66	46	american	american	ADJ
american_scientific_journal-2511	66	47	scientific	scientific	ADJ
american_scientific_journal-2511	66	48	research	research	NOUN
american_scientific_journal-2511	66	49	journal	journal	NOUN
american_scientific_journal-2511	66	50	for	for	ADP
american_scientific_journal-2511	66	51	engineering	engineering	NOUN
american_scientific_journal-2511	66	52	,	,	PUNCT
american_scientific_journal-2511	66	53	technology	technology	NOUN
american_scientific_journal-2511	66	54	,	,	PUNCT
american_scientific_journal-2511	66	55	and	and	CCONJ
american_scientific_journal-2511	66	56	sciences	science	NOUN
american_scientific_journal-2511	66	57	(	(	PUNCT
american_scientific_journal-2511	66	58	asrjets	asrjet	NOUN
american_scientific_journal-2511	66	59	)	)	PUNCT
american_scientific_journal-2511	66	60	(	(	PUNCT
american_scientific_journal-2511	66	61	2017	2017	NUM
american_scientific_journal-2511	66	62	)	)	PUNCT
american_scientific_journal-2511	66	63	volume	volume	NOUN
american_scientific_journal-2511	66	64	27	27	NUM
american_scientific_journal-2511	66	65	,	,	PUNCT
american_scientific_journal-2511	66	66	no	no	DET
american_scientific_journal-2511	66	67	1	1	NUM
american_scientific_journal-2511	66	68	,	,	PUNCT
american_scientific_journal-2511	66	69	pp	pp	ADV
american_scientific_journal-2511	66	70	25	25	NUM
american_scientific_journal-2511	66	71	-	-	SYM
american_scientific_journal-2511	66	72	42	42	NUM
american_scientific_journal-2511	66	73	29	29	NUM
american_scientific_journal-2511	67	1	[	[	X
american_scientific_journal-2511	67	2	10	10	NUM
american_scientific_journal-2511	67	3	]	]	PUNCT
american_scientific_journal-2511	67	4	.	.	PUNCT
american_scientific_journal-2511	68	1	the	the	DET
american_scientific_journal-2511	68	2	goscript	goscript	NOUN
american_scientific_journal-2511	68	3	™	™	ADJ
american_scientific_journal-2511	68	4	reverse	reverse	VERB
american_scientific_journal-2511	68	5	transcription	transcription	NOUN
american_scientific_journal-2511	68	6	system	system	NOUN
american_scientific_journal-2511	68	7	was	be	AUX
american_scientific_journal-2511	68	8	used	use	VERB
american_scientific_journal-2511	68	9	for	for	ADP
american_scientific_journal-2511	68	10	the	the	DET
american_scientific_journal-2511	68	11	synthesis	synthesis	NOUN
american_scientific_journal-2511	68	12	of	of	ADP
american_scientific_journal-2511	68	13	cdna	cdna	PROPN
american_scientific_journal-2511	68	14	from	from	ADP
american_scientific_journal-2511	68	15	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2511	68	16	)	)	PUNCT
american_scientific_journal-2511	68	17	rna	rna	NOUN
american_scientific_journal-2511	68	18	.	.	PUNCT
american_scientific_journal-2511	69	1	the	the	DET
american_scientific_journal-2511	69	2	accupower	accupower	PROPN
american_scientific_journal-2511	69	3	®	®	NOUN
american_scientific_journal-2511	69	4	greenstartm	greenstartm	VERB
american_scientific_journal-2511	69	5	qpcr	qpcr	ADV
american_scientific_journal-2511	69	6	premix	premix	NOUN
american_scientific_journal-2511	69	7	kit	kit	NOUN
american_scientific_journal-2511	69	8	(	(	PUNCT
american_scientific_journal-2511	69	9	bioneer	bioneer	NOUN
american_scientific_journal-2511	69	10	,	,	PUNCT
american_scientific_journal-2511	69	11	korea	korea	PROPN
american_scientific_journal-2511	69	12	)	)	PUNCT
american_scientific_journal-2511	69	13	catalog	catalog	NOUN
american_scientific_journal-2511	69	14	no	no	NOUN
american_scientific_journal-2511	69	15	.	.	PUNCT
american_scientific_journal-2511	70	1	k-6210	k-6210	PROPN
american_scientific_journal-2511	70	2	was	be	AUX
american_scientific_journal-2511	70	3	used	use	VERB
american_scientific_journal-2511	70	4	for	for	ADP
american_scientific_journal-2511	70	5	real	real	ADJ
american_scientific_journal-2511	70	6	time	time	NOUN
american_scientific_journal-2511	70	7	pcr	pcr	ADJ
american_scientific_journal-2511	70	8	reaction	reaction	NOUN
american_scientific_journal-2511	70	9	.	.	PUNCT
american_scientific_journal-2511	71	1	gene	gene	NOUN
american_scientific_journal-2511	71	2	amplification	amplification	NOUN
american_scientific_journal-2511	71	3	in	in	ADP
american_scientific_journal-2511	71	4	real	real	ADJ
american_scientific_journal-2511	71	5	time	time	NOUN
american_scientific_journal-2511	71	6	pcr	pcr	PROPN
american_scientific_journal-2511	71	7	was	be	AUX
american_scientific_journal-2511	71	8	performed	perform	VERB
american_scientific_journal-2511	71	9	in	in	ADP
american_scientific_journal-2511	71	10	a	a	DET
american_scientific_journal-2511	71	11	final	final	ADJ
american_scientific_journal-2511	71	12	volume	volume	NOUN
american_scientific_journal-2511	71	13	of	of	ADP
american_scientific_journal-2511	71	14	20µl	20µl	NOUN
american_scientific_journal-2511	71	15	of	of	ADP
american_scientific_journal-2511	71	16	reaction	reaction	NOUN
american_scientific_journal-2511	71	17	mixtures	mixture	NOUN
american_scientific_journal-2511	71	18	contained	contain	VERB
american_scientific_journal-2511	71	19	1µl	1µl	ADJ
american_scientific_journal-2511	71	20	hr1	hr1	PROPN
american_scientific_journal-2511	71	21	primer	primer	NOUN
american_scientific_journal-2511	71	22	,	,	PUNCT
american_scientific_journal-2511	71	23	1µl	1µl	ADJ
american_scientific_journal-2511	71	24	hr2	hr2	PROPN
american_scientific_journal-2511	71	25	primer	primer	NOUN
american_scientific_journal-2511	71	26	,	,	PUNCT
american_scientific_journal-2511	71	27	6	6	NUM
american_scientific_journal-2511	71	28	µl	µl	ADP
american_scientific_journal-2511	71	29	cdna	cdna	PROPN
american_scientific_journal-2511	71	30	,	,	PUNCT
american_scientific_journal-2511	71	31	10	10	NUM
american_scientific_journal-2511	71	32	µl	µl	ADP
american_scientific_journal-2511	71	33	accupower	accupower	NOUN
american_scientific_journal-2511	71	34	®	®	NOUN
american_scientific_journal-2511	71	35	greenstartm	greenstartm	VERB
american_scientific_journal-2511	71	36	qpcr	qpcr	ADJ
american_scientific_journal-2511	71	37	premix	premix	NOUN
american_scientific_journal-2511	71	38	(	(	PUNCT
american_scientific_journal-2511	71	39	bioneer	bioneer	NOUN
american_scientific_journal-2511	71	40	,	,	PUNCT
american_scientific_journal-2511	71	41	korea	korea	PROPN
american_scientific_journal-2511	71	42	)	)	PUNCT
american_scientific_journal-2511	71	43	and	and	CCONJ
american_scientific_journal-2511	71	44	2	2	NUM
american_scientific_journal-2511	71	45	µl	µl	ADP
american_scientific_journal-2511	71	46	depec	depec	NOUN
american_scientific_journal-2511	71	47	-	-	PUNCT
american_scientific_journal-2511	71	48	distilled	distil	VERB
american_scientific_journal-2511	71	49	water	water	NOUN
american_scientific_journal-2511	71	50	.	.	PUNCT
american_scientific_journal-2511	72	1	the	the	DET
american_scientific_journal-2511	72	2	real	real	ADJ
american_scientific_journal-2511	72	3	-	-	PUNCT
american_scientific_journal-2511	72	4	time	time	NOUN
american_scientific_journal-2511	72	5	pcr	pcr	ADJ
american_scientific_journal-2511	72	6	conditions	condition	NOUN
american_scientific_journal-2511	72	7	were	be	AUX
american_scientific_journal-2511	72	8	:	:	PUNCT
american_scientific_journal-2511	72	9	95	95	NUM
american_scientific_journal-2511	72	10	c	c	NUM
american_scientific_journal-2511	72	11	°	°	NUM
american_scientific_journal-2511	72	12	for	for	ADP
american_scientific_journal-2511	72	13	10	10	NUM
american_scientific_journal-2511	72	14	min	min	NOUN
american_scientific_journal-2511	72	15	.	.	PROPN
american_scientific_journal-2511	72	16	,	,	PUNCT
american_scientific_journal-2511	72	17	95	95	NUM
american_scientific_journal-2511	72	18	c	c	NOUN
american_scientific_journal-2511	72	19	°	°	NUM
american_scientific_journal-2511	72	20	for	for	ADP
american_scientific_journal-2511	72	21	35	35	NUM
american_scientific_journal-2511	72	22	sec	sec	PROPN
american_scientific_journal-2511	72	23	,	,	PUNCT
american_scientific_journal-2511	72	24	63.5	63.5	NUM
american_scientific_journal-2511	72	25	c	c	NOUN
american_scientific_journal-2511	72	26	°	°	NUM
american_scientific_journal-2511	72	27	for	for	ADP
american_scientific_journal-2511	72	28	1	1	NUM
american_scientific_journal-2511	72	29	min	min	NOUN
american_scientific_journal-2511	72	30	and	and	CCONJ
american_scientific_journal-2511	72	31	72	72	NUM
american_scientific_journal-2511	72	32	c	c	NOUN
american_scientific_journal-2511	72	33	°	°	NUM
american_scientific_journal-2511	72	34	for	for	ADP
american_scientific_journal-2511	72	35	1	1	NUM
american_scientific_journal-2511	72	36	min	min	NOUN
american_scientific_journal-2511	72	37	for	for	ADP
american_scientific_journal-2511	72	38	45	45	NUM
american_scientific_journal-2511	72	39	cycles	cycle	NOUN
american_scientific_journal-2511	72	40	.	.	PUNCT
american_scientific_journal-2511	73	1	melting	melt	VERB
american_scientific_journal-2511	73	2	62	62	NUM
american_scientific_journal-2511	73	3	-	-	SYM
american_scientific_journal-2511	73	4	96	96	NUM
american_scientific_journal-2511	73	5	c	c	NOUN
american_scientific_journal-2511	73	6	°	°	NOUN
american_scientific_journal-2511	73	7	every	every	DET
american_scientific_journal-2511	73	8	1	1	NUM
american_scientific_journal-2511	73	9	sec	sec	PROPN
american_scientific_journal-2511	73	10	.	.	PROPN
american_scientific_journal-2511	73	11	2.14	2.14	NUM
american_scientific_journal-2511	73	12	purification	purification	NOUN
american_scientific_journal-2511	73	13	by	by	ADP
american_scientific_journal-2511	73	14	using	use	VERB
american_scientific_journal-2511	73	15	hislink	hislink	NOUN
american_scientific_journal-2511	73	16	™	™	ADJ
american_scientific_journal-2511	73	17	protein	protein	NOUN
american_scientific_journal-2511	73	18	purification	purification	NOUN
american_scientific_journal-2511	73	19	resin	resin	NOUN
american_scientific_journal-2511	73	20	(	(	PUNCT
american_scientific_journal-2511	73	21	promega	promega	PROPN
american_scientific_journal-2511	73	22	,	,	PUNCT
american_scientific_journal-2511	73	23	usa	usa	PROPN
american_scientific_journal-2511	73	24	)	)	PUNCT
american_scientific_journal-2511	73	25	(	(	PUNCT
american_scientific_journal-2511	73	26	immobilized	immobilize	VERB
american_scientific_journal-2511	73	27	metal	metal	NOUN
american_scientific_journal-2511	73	28	affinity	affinity	NOUN
american_scientific_journal-2511	73	29	chromatography	chromatography	NOUN
american_scientific_journal-2511	73	30	with	with	ADP
american_scientific_journal-2511	73	31	modified	modify	VERB
american_scientific_journal-2511	73	32	)	)	PUNCT
american_scientific_journal-2511	73	33	because	because	SCONJ
american_scientific_journal-2511	73	34	the	the	DET
american_scientific_journal-2511	73	35	produced	produce	VERB
american_scientific_journal-2511	73	36	hirudin	hirudin	NOUN
american_scientific_journal-2511	73	37	contain	contain	VERB
american_scientific_journal-2511	73	38	his	his	PRON
american_scientific_journal-2511	73	39	-	-	PUNCT
american_scientific_journal-2511	73	40	tag	tag	NOUN
american_scientific_journal-2511	73	41	as	as	ADP
american_scientific_journal-2511	73	42	a	a	DET
american_scientific_journal-2511	73	43	result	result	NOUN
american_scientific_journal-2511	73	44	for	for	ADP
american_scientific_journal-2511	73	45	using	use	VERB
american_scientific_journal-2511	73	46	pet-16b	pet-16b	NUM
american_scientific_journal-2511	73	47	vector	vector	NOUN
american_scientific_journal-2511	73	48	,	,	PUNCT
american_scientific_journal-2511	73	49	therefore	therefore	ADV
american_scientific_journal-2511	73	50	,	,	PUNCT
american_scientific_journal-2511	73	51	using	use	VERB
american_scientific_journal-2511	73	52	of	of	ADP
american_scientific_journal-2511	73	53	imac	imac	NOUN
american_scientific_journal-2511	73	54	is	be	AUX
american_scientific_journal-2511	73	55	very	very	ADV
american_scientific_journal-2511	73	56	effective	effective	ADJ
american_scientific_journal-2511	73	57	.	.	PUNCT
american_scientific_journal-2511	74	1	this	this	DET
american_scientific_journal-2511	74	2	process	process	NOUN
american_scientific_journal-2511	74	3	has	have	AUX
american_scientific_journal-2511	74	4	been	be	AUX
american_scientific_journal-2511	74	5	done	do	VERB
american_scientific_journal-2511	74	6	after	after	ADP
american_scientific_journal-2511	74	7	cell	cell	NOUN
american_scientific_journal-2511	74	8	preparation	preparation	NOUN
american_scientific_journal-2511	74	9	of	of	ADP
american_scientific_journal-2511	74	10	cell	cell	NOUN
american_scientific_journal-2511	74	11	lysate	lysate	NOUN
american_scientific_journal-2511	74	12	by	by	ADP
american_scientific_journal-2511	74	13	using	use	VERB
american_scientific_journal-2511	74	14	suspension	suspension	NOUN
american_scientific_journal-2511	74	15	buffer	buffer	NOUN
american_scientific_journal-2511	74	16	(	(	PUNCT
american_scientific_journal-2511	74	17	100	100	NUM
american_scientific_journal-2511	74	18	mm	mm	NUM
american_scientific_journal-2511	74	19	tris	tris	PROPN
american_scientific_journal-2511	74	20	-	-	PUNCT
american_scientific_journal-2511	74	21	hcl	hcl	NOUN
american_scientific_journal-2511	74	22	,	,	PUNCT
american_scientific_journal-2511	74	23	8	8	NUM
american_scientific_journal-2511	74	24	mm	mm	NOUN
american_scientific_journal-2511	74	25	urea	urea	NOUN
american_scientific_journal-2511	74	26	,	,	PUNCT
american_scientific_journal-2511	74	27	300	300	NUM
american_scientific_journal-2511	74	28	mm	mm	NOUN
american_scientific_journal-2511	74	29	nacl	nacl	NOUN
american_scientific_journal-2511	74	30	,	,	PUNCT
american_scientific_journal-2511	74	31	ph	ph	NOUN
american_scientific_journal-2511	74	32	7.5	7.5	NUM
american_scientific_journal-2511	74	33	)	)	PUNCT
american_scientific_journal-2511	74	34	with	with	ADP
american_scientific_journal-2511	74	35	(	(	PUNCT
american_scientific_journal-2511	74	36	0.1	0.1	NUM
american_scientific_journal-2511	74	37	%	%	NOUN
american_scientific_journal-2511	74	38	lysosyme	lysosyme	NOUN
american_scientific_journal-2511	74	39	,	,	PUNCT
american_scientific_journal-2511	74	40	20	20	NUM
american_scientific_journal-2511	74	41	mm	mm	NOUN
american_scientific_journal-2511	74	42	pmsf	pmsf	NOUN
american_scientific_journal-2511	74	43	and	and	CCONJ
american_scientific_journal-2511	74	44	β	β	NOUN
american_scientific_journal-2511	74	45	-	-	NOUN
american_scientific_journal-2511	74	46	mercaptoethanol	mercaptoethanol	NOUN
american_scientific_journal-2511	74	47	)	)	PUNCT
american_scientific_journal-2511	74	48	then	then	ADV
american_scientific_journal-2511	74	49	,	,	PUNCT
american_scientific_journal-2511	74	50	incubated	incubate	VERB
american_scientific_journal-2511	74	51	for	for	ADP
american_scientific_journal-2511	74	52	20min	20min	NOUN
american_scientific_journal-2511	74	53	.	.	PUNCT
american_scientific_journal-2511	75	1	at	at	ADP
american_scientific_journal-2511	75	2	37c	37c	NUM
american_scientific_journal-2511	75	3	°	°	NOUN
american_scientific_journal-2511	75	4	.	.	PUNCT
american_scientific_journal-2511	76	1	for	for	ADP
american_scientific_journal-2511	76	2	homogenizing	homogenize	VERB
american_scientific_journal-2511	76	3	the	the	DET
american_scientific_journal-2511	76	4	sample	sample	NOUN
american_scientific_journal-2511	76	5	,	,	PUNCT
american_scientific_journal-2511	76	6	the	the	DET
american_scientific_journal-2511	76	7	sonication	sonication	NOUN
american_scientific_journal-2511	76	8	burst	burst	NOUN
american_scientific_journal-2511	76	9	was	be	AUX
american_scientific_journal-2511	76	10	given	give	VERB
american_scientific_journal-2511	76	11	for	for	ADP
american_scientific_journal-2511	76	12	15	15	NUM
american_scientific_journal-2511	76	13	min	min	NOUN
american_scientific_journal-2511	76	14	.	.	PROPN
american_scientific_journal-2511	76	15	at	at	ADP
american_scientific_journal-2511	76	16	4c	4c	NOUN
american_scientific_journal-2511	76	17	°	°	NOUN
american_scientific_journal-2511	76	18	at	at	ADP
american_scientific_journal-2511	76	19	60	60	NUM
american_scientific_journal-2511	76	20	hz	hz	NOUN
american_scientific_journal-2511	76	21	.	.	PUNCT
american_scientific_journal-2511	76	22	intensity	intensity	NOUN
american_scientific_journal-2511	76	23	then	then	ADV
american_scientific_journal-2511	76	24	centrifuged	centrifuge	VERB
american_scientific_journal-2511	76	25	to	to	PART
american_scientific_journal-2511	76	26	remove	remove	VERB
american_scientific_journal-2511	76	27	unbroken	unbroken	ADJ
american_scientific_journal-2511	76	28	cells	cell	NOUN
american_scientific_journal-2511	76	29	debris	debris	NOUN
american_scientific_journal-2511	76	30	.	.	PUNCT
american_scientific_journal-2511	77	1	the	the	DET
american_scientific_journal-2511	77	2	supernatant	supernatant	NOUN
american_scientific_journal-2511	77	3	was	be	AUX
american_scientific_journal-2511	77	4	loaded	load	VERB
american_scientific_journal-2511	77	5	onto	onto	ADP
american_scientific_journal-2511	77	6	column	column	NOUN
american_scientific_journal-2511	77	7	packed	pack	VERB
american_scientific_journal-2511	77	8	with	with	ADP
american_scientific_journal-2511	77	9	ni2	ni2	NOUN
american_scientific_journal-2511	77	10	+	+	ADJ
american_scientific_journal-2511	77	11	-chelating	-chelating	ADJ
american_scientific_journal-2511	77	12	resin	resin	NOUN
american_scientific_journal-2511	77	13	(	(	PUNCT
american_scientific_journal-2511	77	14	promega	promega	NOUN
american_scientific_journal-2511	77	15	)	)	PUNCT
american_scientific_journal-2511	77	16	by	by	ADP
american_scientific_journal-2511	77	17	using	use	VERB
american_scientific_journal-2511	77	18	binding	bind	VERB
american_scientific_journal-2511	77	19	buffer	buffer	NOUN
american_scientific_journal-2511	77	20	(	(	PUNCT
american_scientific_journal-2511	77	21	100	100	NUM
american_scientific_journal-2511	77	22	mm	mm	NUM
american_scientific_journal-2511	77	23	tris	tris	PROPN
american_scientific_journal-2511	77	24	-	-	PUNCT
american_scientific_journal-2511	77	25	hcl	hcl	NOUN
american_scientific_journal-2511	77	26	,	,	PUNCT
american_scientific_journal-2511	77	27	8	8	NUM
american_scientific_journal-2511	77	28	mm	mm	NOUN
american_scientific_journal-2511	77	29	urea	urea	NOUN
american_scientific_journal-2511	77	30	,	,	PUNCT
american_scientific_journal-2511	77	31	300	300	NUM
american_scientific_journal-2511	77	32	mm	mm	NOUN
american_scientific_journal-2511	77	33	nacl	nacl	NOUN
american_scientific_journal-2511	77	34	,	,	PUNCT
american_scientific_journal-2511	77	35	10	10	NUM
american_scientific_journal-2511	77	36	mm	mm	NOUN
american_scientific_journal-2511	77	37	imidazol	imidazol	NOUN
american_scientific_journal-2511	77	38	,	,	PUNCT
american_scientific_journal-2511	77	39	ph	ph	ADJ
american_scientific_journal-2511	77	40	7.5	7.5	NUM
american_scientific_journal-2511	77	41	)	)	PUNCT
american_scientific_journal-2511	77	42	and	and	CCONJ
american_scientific_journal-2511	77	43	protein	protein	NOUN
american_scientific_journal-2511	77	44	was	be	AUX
american_scientific_journal-2511	77	45	eluted	elute	VERB
american_scientific_journal-2511	77	46	with	with	ADP
american_scientific_journal-2511	77	47	buffer	buffer	NOUN
american_scientific_journal-2511	77	48	(	(	PUNCT
american_scientific_journal-2511	77	49	100	100	NUM
american_scientific_journal-2511	77	50	mm	mm	NUM
american_scientific_journal-2511	77	51	tris	tris	PROPN
american_scientific_journal-2511	77	52	-	-	PUNCT
american_scientific_journal-2511	77	53	hcl	hcl	NOUN
american_scientific_journal-2511	77	54	,	,	PUNCT
american_scientific_journal-2511	77	55	500	500	NUM
american_scientific_journal-2511	77	56	mm	mm	NOUN
american_scientific_journal-2511	77	57	imidazol	imidazol	NOUN
american_scientific_journal-2511	77	58	,	,	PUNCT
american_scientific_journal-2511	77	59	ph	ph	ADJ
american_scientific_journal-2511	77	60	7.5	7.5	NUM
american_scientific_journal-2511	77	61	)	)	PUNCT
american_scientific_journal-2511	77	62	.	.	PUNCT
american_scientific_journal-2511	78	1	the	the	DET
american_scientific_journal-2511	78	2	eluted	eluted	ADJ
american_scientific_journal-2511	78	3	protein	protein	NOUN
american_scientific_journal-2511	78	4	collected	collect	VERB
american_scientific_journal-2511	78	5	and	and	CCONJ
american_scientific_journal-2511	78	6	analyzed	analyze	VERB
american_scientific_journal-2511	78	7	by	by	ADP
american_scientific_journal-2511	78	8	15%sds	15%sds	PROPN
american_scientific_journal-2511	78	9	-	-	PUNCT
american_scientific_journal-2511	78	10	page	page	NOUN
american_scientific_journal-2511	78	11	.	.	PUNCT
american_scientific_journal-2511	79	1	2.15	2.15	NUM
american_scientific_journal-2511	79	2	dialysis	dialysis	NOUN
american_scientific_journal-2511	79	3	protein	protein	NOUN
american_scientific_journal-2511	79	4	produced	produce	VERB
american_scientific_journal-2511	79	5	after	after	SCONJ
american_scientific_journal-2511	79	6	affinity	affinity	NOUN
american_scientific_journal-2511	79	7	chromatography	chromatography	NOUN
american_scientific_journal-2511	79	8	was	be	AUX
american_scientific_journal-2511	79	9	dialyzed	dialyze	VERB
american_scientific_journal-2511	79	10	with	with	ADP
american_scientific_journal-2511	79	11	10x	10x	NOUN
american_scientific_journal-2511	79	12	solution	solution	NOUN
american_scientific_journal-2511	79	13	(	(	PUNCT
american_scientific_journal-2511	79	14	100	100	NUM
american_scientific_journal-2511	79	15	mm	mm	NOUN
american_scientific_journal-2511	79	16	tris	tris	NOUN
american_scientific_journal-2511	79	17	-	-	PUNCT
american_scientific_journal-2511	79	18	hcl	hcl	NOUN
american_scientific_journal-2511	79	19	,	,	PUNCT
american_scientific_journal-2511	79	20	100	100	NUM
american_scientific_journal-2511	79	21	mm	mm	NOUN
american_scientific_journal-2511	79	22	nacl	nacl	NOUN
american_scientific_journal-2511	79	23	,	,	PUNCT
american_scientific_journal-2511	79	24	10	10	NUM
american_scientific_journal-2511	79	25	%	%	NOUN
american_scientific_journal-2511	79	26	v	v	ADP
american_scientific_journal-2511	79	27	/	/	SYM
american_scientific_journal-2511	79	28	v	v	NOUN
american_scientific_journal-2511	79	29	glycerol	glycerol	NOUN
american_scientific_journal-2511	79	30	,	,	PUNCT
american_scientific_journal-2511	79	31	0.1	0.1	NUM
american_scientific_journal-2511	79	32	mm	mm	NOUN
american_scientific_journal-2511	79	33	edta	edta	NOUN
american_scientific_journal-2511	79	34	,	,	PUNCT
american_scientific_journal-2511	79	35	ph	ph	VERB
american_scientific_journal-2511	79	36	7.5	7.5	NUM
american_scientific_journal-2511	79	37	for	for	ADP
american_scientific_journal-2511	79	38	4h	4h	NUM
american_scientific_journal-2511	79	39	at	at	ADP
american_scientific_journal-2511	79	40	4c	4c	NOUN
american_scientific_journal-2511	79	41	°	°	NOUN
american_scientific_journal-2511	79	42	and	and	CCONJ
american_scientific_journal-2511	79	43	with	with	ADP
american_scientific_journal-2511	79	44	100x	100x	NUM
american_scientific_journal-2511	79	45	from	from	ADP
american_scientific_journal-2511	79	46	the	the	DET
american_scientific_journal-2511	79	47	same	same	ADJ
american_scientific_journal-2511	79	48	solution	solution	NOUN
american_scientific_journal-2511	79	49	for	for	ADP
american_scientific_journal-2511	79	50	16h	16h	NUM
american_scientific_journal-2511	79	51	at	at	ADP
american_scientific_journal-2511	79	52	4c	4c	NOUN
american_scientific_journal-2511	79	53	°	°	NOUN
american_scientific_journal-2511	80	1	[	[	X
american_scientific_journal-2511	80	2	11	11	NUM
american_scientific_journal-2511	80	3	]	]	PUNCT
american_scientific_journal-2511	80	4	.	.	PUNCT
american_scientific_journal-2511	81	1	2.16	2.16	NUM
american_scientific_journal-2511	81	2	treatment	treatment	NOUN
american_scientific_journal-2511	81	3	with	with	ADP
american_scientific_journal-2511	81	4	factor	factor	NOUN
american_scientific_journal-2511	81	5	xa	xa	PROPN
american_scientific_journal-2511	81	6	to	to	PART
american_scientific_journal-2511	81	7	remove	remove	VERB
american_scientific_journal-2511	81	8	his	his	PRON
american_scientific_journal-2511	81	9	-	-	PUNCT
american_scientific_journal-2511	81	10	tag	tag	NOUN
american_scientific_journal-2511	81	11	from	from	ADP
american_scientific_journal-2511	81	12	produced	produce	VERB
american_scientific_journal-2511	81	13	protein	protein	NOUN
american_scientific_journal-2511	81	14	,	,	PUNCT
american_scientific_journal-2511	81	15	factor	factor	NOUN
american_scientific_journal-2511	81	16	xa	xa	PROPN
american_scientific_journal-2511	81	17	(	(	PUNCT
american_scientific_journal-2511	81	18	biolab	biolab	PROPN
american_scientific_journal-2511	81	19	,	,	PUNCT
american_scientific_journal-2511	81	20	u.k	u.k	PROPN
american_scientific_journal-2511	81	21	.	.	PROPN
american_scientific_journal-2511	81	22	)	)	PUNCT
american_scientific_journal-2511	81	23	was	be	AUX
american_scientific_journal-2511	81	24	used	use	VERB
american_scientific_journal-2511	81	25	by	by	ADP
american_scientific_journal-2511	81	26	incubation	incubation	NOUN
american_scientific_journal-2511	81	27	for	for	ADP
american_scientific_journal-2511	81	28	6h	6h	NUM
american_scientific_journal-2511	81	29	at	at	ADP
american_scientific_journal-2511	81	30	23c	23c	NOUN
american_scientific_journal-2511	81	31	°	°	ADP
american_scientific_journal-2511	81	32	to	to	PART
american_scientific_journal-2511	81	33	obtain	obtain	VERB
american_scientific_journal-2511	81	34	hirudin	hirudin	NOUN
american_scientific_journal-2511	81	35	without	without	ADP
american_scientific_journal-2511	81	36	his	his	PRON
american_scientific_journal-2511	81	37	-	-	PUNCT
american_scientific_journal-2511	81	38	tag	tag	NOUN
american_scientific_journal-2511	81	39	.	.	PUNCT
american_scientific_journal-2511	82	1	2.17	2.17	NUM
american_scientific_journal-2511	82	2	purification	purification	NOUN
american_scientific_journal-2511	82	3	by	by	ADP
american_scientific_journal-2511	82	4	deae	deae	NOUN
american_scientific_journal-2511	82	5	sepharose	sepharose	NOUN
american_scientific_journal-2511	82	6	column	column	NOUN
american_scientific_journal-2511	82	7	(	(	PUNCT
american_scientific_journal-2511	82	8	anion	anion	NOUN
american_scientific_journal-2511	82	9	-	-	PUNCT
american_scientific_journal-2511	82	10	exchange	exchange	NOUN
american_scientific_journal-2511	82	11	chromatography	chromatography	NOUN
american_scientific_journal-2511	82	12	)	)	PUNCT
american_scientific_journal-2511	82	13	hitrap	hitrap	NOUN
american_scientific_journal-2511	82	14	™	™	ADJ
american_scientific_journal-2511	82	15	deae	deae	ADP
american_scientific_journal-2511	82	16	ff,1ml	ff,1ml	ADJ
american_scientific_journal-2511	82	17	column	column	NOUN
american_scientific_journal-2511	82	18	supplied	supply	VERB
american_scientific_journal-2511	82	19	by	by	ADP
american_scientific_journal-2511	82	20	(	(	PUNCT
american_scientific_journal-2511	82	21	gehealthcare	gehealthcare	PROPN
american_scientific_journal-2511	82	22	,	,	PUNCT
american_scientific_journal-2511	82	23	sweden	sweden	PROPN
american_scientific_journal-2511	82	24	)	)	PUNCT
american_scientific_journal-2511	82	25	was	be	AUX
american_scientific_journal-2511	82	26	used	use	VERB
american_scientific_journal-2511	82	27	which	which	PRON
american_scientific_journal-2511	82	28	equilibrated	equilibrate	VERB
american_scientific_journal-2511	82	29	with	with	ADP
american_scientific_journal-2511	82	30	(	(	PUNCT
american_scientific_journal-2511	82	31	20	20	NUM
american_scientific_journal-2511	82	32	mm	mm	PROPN
american_scientific_journal-2511	82	33	tris	tris	NOUN
american_scientific_journal-2511	82	34	-	-	PUNCT
american_scientific_journal-2511	82	35	hcl	hcl	NOUN
american_scientific_journal-2511	82	36	ph=8	ph=8	NOUN
american_scientific_journal-2511	82	37	)	)	PUNCT
american_scientific_journal-2511	82	38	then	then	ADV
american_scientific_journal-2511	82	39	,	,	PUNCT
american_scientific_journal-2511	82	40	hirudin	hirudin	VERB
american_scientific_journal-2511	82	41	eluted	elute	VERB
american_scientific_journal-2511	82	42	with	with	ADP
american_scientific_journal-2511	82	43	[	[	X
american_scientific_journal-2511	82	44	20	20	NUM
american_scientific_journal-2511	82	45	mm	mm	NOUN
american_scientific_journal-2511	82	46	tris	tris	NOUN
american_scientific_journal-2511	82	47	-	-	PUNCT
american_scientific_journal-2511	82	48	hcl	hcl	PROPN
american_scientific_journal-2511	82	49	with	with	ADP
american_scientific_journal-2511	82	50	gradients	gradient	NOUN
american_scientific_journal-2511	82	51	of	of	ADP
american_scientific_journal-2511	82	52	(	(	PUNCT
american_scientific_journal-2511	82	53	0.1	0.1	NUM
american_scientific_journal-2511	82	54	-	-	SYM
american_scientific_journal-2511	82	55	0.5	0.5	NUM
american_scientific_journal-2511	82	56	m	m	NOUN
american_scientific_journal-2511	82	57	nacl	nacl	NOUN
american_scientific_journal-2511	82	58	)	)	PUNCT
american_scientific_journal-2511	82	59	,	,	PUNCT
american_scientific_journal-2511	82	60	ph	ph	VERB
american_scientific_journal-2511	82	61	8	8	NUM
american_scientific_journal-2511	82	62	]	]	PUNCT
american_scientific_journal-2511	82	63	.	.	PUNCT
american_scientific_journal-2511	83	1	the	the	DET
american_scientific_journal-2511	83	2	product	product	NOUN
american_scientific_journal-2511	83	3	analyzed	analyze	VERB
american_scientific_journal-2511	83	4	by	by	ADP
american_scientific_journal-2511	83	5	15	15	NUM
american_scientific_journal-2511	83	6	%	%	NOUN
american_scientific_journal-2511	83	7	sds	sds	NOUN
american_scientific_journal-2511	83	8	-	-	PUNCT
american_scientific_journal-2511	83	9	page	page	NOUN
american_scientific_journal-2511	83	10	.	.	PUNCT
american_scientific_journal-2511	84	1	2.18	2.18	NUM
american_scientific_journal-2511	84	2	purification	purification	NOUN
american_scientific_journal-2511	84	3	by	by	ADP
american_scientific_journal-2511	84	4	sp	sp	ADP
american_scientific_journal-2511	84	5	sepharose	sepharose	NOUN
american_scientific_journal-2511	84	6	column	column	NOUN
american_scientific_journal-2511	84	7	(	(	PUNCT
american_scientific_journal-2511	84	8	cation	cation	NOUN
american_scientific_journal-2511	84	9	-	-	PUNCT
american_scientific_journal-2511	84	10	exchange	exchange	NOUN
american_scientific_journal-2511	84	11	chromatography	chromatography	NOUN
american_scientific_journal-2511	84	12	)	)	PUNCT
american_scientific_journal-2511	84	13	hitrap	hitrap	NOUN
american_scientific_journal-2511	84	14	™	™	NOUN
american_scientific_journal-2511	84	15	sp	sp	ADP
american_scientific_journal-2511	84	16	ff,1ml	ff,1ml	NOUN
american_scientific_journal-2511	84	17	column	column	NOUN
american_scientific_journal-2511	84	18	supplied	supply	VERB
american_scientific_journal-2511	84	19	by	by	ADP
american_scientific_journal-2511	84	20	(	(	PUNCT
american_scientific_journal-2511	84	21	gehealthcare	gehealthcare	PROPN
american_scientific_journal-2511	84	22	,	,	PUNCT
american_scientific_journal-2511	84	23	sweden	sweden	PROPN
american_scientific_journal-2511	84	24	)	)	PUNCT
american_scientific_journal-2511	84	25	was	be	AUX
american_scientific_journal-2511	84	26	used	use	VERB
american_scientific_journal-2511	84	27	,	,	PUNCT
american_scientific_journal-2511	84	28	the	the	DET
american_scientific_journal-2511	84	29	penetration	penetration	NOUN
american_scientific_journal-2511	84	30	collected	collect	VERB
american_scientific_journal-2511	84	31	from	from	ADP
american_scientific_journal-2511	84	32	deae	deae	NOUN
american_scientific_journal-2511	84	33	column	column	NOUN
american_scientific_journal-2511	84	34	and	and	CCONJ
american_scientific_journal-2511	84	35	loaded	load	VERB
american_scientific_journal-2511	84	36	to	to	ADP
american_scientific_journal-2511	84	37	sp	sp	ADP
american_scientific_journal-2511	84	38	column	column	NOUN
american_scientific_journal-2511	84	39	which	which	PRON
american_scientific_journal-2511	84	40	equilibrated	equilibrate	VERB
american_scientific_journal-2511	84	41	with	with	ADP
american_scientific_journal-2511	84	42	(	(	PUNCT
american_scientific_journal-2511	84	43	ammonium	ammonium	NOUN
american_scientific_journal-2511	84	44	acetate	acetate	NOUN
american_scientific_journal-2511	84	45	ph	ph	PROPN
american_scientific_journal-2511	84	46	4.5	4.5	NUM
american_scientific_journal-2511	84	47	)	)	PUNCT
american_scientific_journal-2511	84	48	,	,	PUNCT
american_scientific_journal-2511	84	49	washed	wash	VERB
american_scientific_journal-2511	84	50	with	with	ADP
american_scientific_journal-2511	84	51	(	(	PUNCT
american_scientific_journal-2511	84	52	ammonium	ammonium	NOUN
american_scientific_journal-2511	84	53	acetate	acetate	NOUN
american_scientific_journal-2511	84	54	ph	ph	PROPN
american_scientific_journal-2511	84	55	7	7	NUM
american_scientific_journal-2511	84	56	)	)	PUNCT
american_scientific_journal-2511	84	57	then	then	ADV
american_scientific_journal-2511	84	58	protein	protein	NOUN
american_scientific_journal-2511	84	59	eluted	elute	VERB
american_scientific_journal-2511	84	60	with	with	ADP
american_scientific_journal-2511	84	61	(	(	PUNCT
american_scientific_journal-2511	84	62	ammonium	ammonium	NOUN
american_scientific_journal-2511	84	63	acetate	acetate	NOUN
american_scientific_journal-2511	84	64	ph	ph	PROPN
american_scientific_journal-2511	84	65	8)	8)	PROPN
american_scientific_journal-2511	84	66	.	.	PUNCT
american_scientific_journal-2511	85	1	the	the	DET
american_scientific_journal-2511	85	2	product	product	NOUN
american_scientific_journal-2511	85	3	analyzed	analyze	VERB
american_scientific_journal-2511	85	4	by	by	ADP
american_scientific_journal-2511	85	5	15	15	NUM
american_scientific_journal-2511	85	6	%	%	NOUN
american_scientific_journal-2511	85	7	sds	sds	NOUN
american_scientific_journal-2511	85	8	-	-	PUNCT
american_scientific_journal-2511	85	9	page	page	NOUN
american_scientific_journal-2511	85	10	.	.	PUNCT
american_scientific_journal-2511	86	1	american	american	ADJ
american_scientific_journal-2511	86	2	scientific	scientific	ADJ
american_scientific_journal-2511	86	3	research	research	PROPN
american_scientific_journal-2511	86	4	journal	journal	NOUN
american_scientific_journal-2511	86	5	for	for	ADP
american_scientific_journal-2511	86	6	engineering	engineering	NOUN
american_scientific_journal-2511	86	7	,	,	PUNCT
american_scientific_journal-2511	86	8	technology	technology	NOUN
american_scientific_journal-2511	86	9	,	,	PUNCT
american_scientific_journal-2511	86	10	and	and	CCONJ
american_scientific_journal-2511	86	11	sciences	science	NOUN
american_scientific_journal-2511	86	12	(	(	PUNCT
american_scientific_journal-2511	86	13	asrjets	asrjet	NOUN
american_scientific_journal-2511	86	14	)	)	PUNCT
american_scientific_journal-2511	86	15	(	(	PUNCT
american_scientific_journal-2511	86	16	2017	2017	NUM
american_scientific_journal-2511	86	17	)	)	PUNCT
american_scientific_journal-2511	86	18	volume	volume	NOUN
american_scientific_journal-2511	86	19	27	27	NUM
american_scientific_journal-2511	86	20	,	,	PUNCT
american_scientific_journal-2511	86	21	no	no	DET
american_scientific_journal-2511	86	22	1	1	NUM
american_scientific_journal-2511	86	23	,	,	PUNCT
american_scientific_journal-2511	86	24	pp	pp	ADV
american_scientific_journal-2511	86	25	25	25	NUM
american_scientific_journal-2511	86	26	-	-	SYM
american_scientific_journal-2511	86	27	42	42	NUM
american_scientific_journal-2511	86	28	30	30	NUM
american_scientific_journal-2511	86	29	2.19	2.19	NUM
american_scientific_journal-2511	86	30	hirudin	hirudin	VERB
american_scientific_journal-2511	86	31	enzyme	enzyme	NOUN
american_scientific_journal-2511	86	32	immunoassay	immunoassay	ADJ
american_scientific_journal-2511	86	33	test	test	NOUN
american_scientific_journal-2511	86	34	human	human	ADJ
american_scientific_journal-2511	86	35	hirudin	hirudin	NOUN
american_scientific_journal-2511	86	36	elisa	elisa	NOUN
american_scientific_journal-2511	86	37	kit	kit	NOUN
american_scientific_journal-2511	86	38	was	be	AUX
american_scientific_journal-2511	86	39	used	use	VERB
american_scientific_journal-2511	86	40	for	for	ADP
american_scientific_journal-2511	86	41	the	the	DET
american_scientific_journal-2511	86	42	quantitative	quantitative	NOUN
american_scientific_journal-2511	86	43	in	in	ADP
american_scientific_journal-2511	86	44	vitro	vitro	X
american_scientific_journal-2511	86	45	diagnostic	diagnostic	ADJ
american_scientific_journal-2511	86	46	measurement	measurement	NOUN
american_scientific_journal-2511	86	47	of	of	ADP
american_scientific_journal-2511	86	48	hirudin	hirudin	VERB
american_scientific_journal-2511	86	49	in	in	ADP
american_scientific_journal-2511	86	50	the	the	DET
american_scientific_journal-2511	86	51	solution	solution	NOUN
american_scientific_journal-2511	86	52	by	by	ADP
american_scientific_journal-2511	86	53	an	an	DET
american_scientific_journal-2511	86	54	enzyme	enzyme	NOUN
american_scientific_journal-2511	86	55	immunoassay	immunoassay	NOUN
american_scientific_journal-2511	86	56	,	,	PUNCT
american_scientific_journal-2511	86	57	according	accord	VERB
american_scientific_journal-2511	86	58	to	to	ADP
american_scientific_journal-2511	86	59	the	the	DET
american_scientific_journal-2511	86	60	manufacture	manufacture	NOUN
american_scientific_journal-2511	86	61	protocol	protocol	NOUN
american_scientific_journal-2511	86	62	(	(	PUNCT
american_scientific_journal-2511	86	63	mybiosource	mybiosource	PROPN
american_scientific_journal-2511	86	64	,	,	PUNCT
american_scientific_journal-2511	86	65	usa	usa	PROPN
american_scientific_journal-2511	86	66	)	)	PUNCT
american_scientific_journal-2511	86	67	catolog	catolog	NOUN
american_scientific_journal-2511	86	68	no	no	INTJ
american_scientific_journal-2511	86	69	.	.	PUNCT
american_scientific_journal-2511	86	70	mbs262224	mbs262224	X
american_scientific_journal-2511	86	71	2.20	2.20	NUM
american_scientific_journal-2511	86	72	bioassay	bioassay	VERB
american_scientific_journal-2511	86	73	the	the	DET
american_scientific_journal-2511	86	74	activity	activity	NOUN
american_scientific_journal-2511	86	75	of	of	ADP
american_scientific_journal-2511	86	76	hirudin	hirudin	VERB
american_scientific_journal-2511	86	77	(	(	PUNCT
american_scientific_journal-2511	86	78	hirudin	hirudin	VERB
american_scientific_journal-2511	86	79	titration	titration	NOUN
american_scientific_journal-2511	86	80	with	with	ADP
american_scientific_journal-2511	86	81	thrombin	thrombin	NOUN
american_scientific_journal-2511	86	82	)	)	PUNCT
american_scientific_journal-2511	86	83	the	the	DET
american_scientific_journal-2511	86	84	experiment	experiment	NOUN
american_scientific_journal-2511	86	85	has	have	AUX
american_scientific_journal-2511	86	86	been	be	AUX
american_scientific_journal-2511	86	87	done	do	VERB
american_scientific_journal-2511	86	88	according	accord	VERB
american_scientific_journal-2511	86	89	to	to	ADP
american_scientific_journal-2511	86	90	markwardt	markwardt	ADJ
american_scientific_journal-2511	86	91	[	[	X
american_scientific_journal-2511	86	92	12	12	NUM
american_scientific_journal-2511	86	93	]	]	PUNCT
american_scientific_journal-2511	86	94	by	by	ADP
american_scientific_journal-2511	86	95	using	use	VERB
american_scientific_journal-2511	86	96	0.5	0.5	NUM
american_scientific_journal-2511	86	97	%	%	NOUN
american_scientific_journal-2511	86	98	fibrinogen	fibrinogen	NOUN
american_scientific_journal-2511	86	99	(	(	PUNCT
american_scientific_journal-2511	86	100	bioworld	bioworld	NOUN
american_scientific_journal-2511	86	101	,	,	PUNCT
american_scientific_journal-2511	86	102	usa	usa	PROPN
american_scientific_journal-2511	86	103	)	)	PUNCT
american_scientific_journal-2511	86	104	and	and	CCONJ
american_scientific_journal-2511	86	105	(	(	PUNCT
american_scientific_journal-2511	86	106	100	100	NUM
american_scientific_journal-2511	86	107	nih	nih	PROPN
american_scientific_journal-2511	86	108	unit	unit	PROPN
american_scientific_journal-2511	86	109	/	/	SYM
american_scientific_journal-2511	86	110	ml	ml	PROPN
american_scientific_journal-2511	86	111	)	)	PUNCT
american_scientific_journal-2511	86	112	thrombin	thrombin	NOUN
american_scientific_journal-2511	86	113	solution	solution	NOUN
american_scientific_journal-2511	86	114	(	(	PUNCT
american_scientific_journal-2511	86	115	sigma	sigma	PROPN
american_scientific_journal-2511	86	116	,	,	PUNCT
american_scientific_journal-2511	86	117	usa	usa	PROPN
american_scientific_journal-2511	86	118	)	)	PUNCT
american_scientific_journal-2511	86	119	.	.	PUNCT
american_scientific_journal-2511	87	1	3	3	X
american_scientific_journal-2511	87	2	.	.	NOUN
american_scientific_journal-2511	87	3	results	result	VERB
american_scientific_journal-2511	87	4	3.1	3.1	NUM
american_scientific_journal-2511	87	5	visualization	visualization	NOUN
american_scientific_journal-2511	87	6	of	of	ADP
american_scientific_journal-2511	87	7	purified	purified	ADJ
american_scientific_journal-2511	87	8	18srdna	18srdna	PROPN
american_scientific_journal-2511	87	9	and	and	CCONJ
american_scientific_journal-2511	87	10	co	co	NOUN
american_scientific_journal-2511	87	11	-	-	NOUN
american_scientific_journal-2511	87	12	i	i	PRON
american_scientific_journal-2511	87	13	genes	gene	NOUN
american_scientific_journal-2511	87	14	on	on	ADP
american_scientific_journal-2511	87	15	agarose	agarose	NOUN
american_scientific_journal-2511	87	16	gels	gel	NOUN
american_scientific_journal-2511	87	17	the	the	DET
american_scientific_journal-2511	87	18	amplified	amplified	ADJ
american_scientific_journal-2511	87	19	products	product	NOUN
american_scientific_journal-2511	87	20	of	of	ADP
american_scientific_journal-2511	87	21	18s	18	NOUN
american_scientific_journal-2511	87	22	rdna	rdna	PROPN
american_scientific_journal-2511	87	23	and	and	CCONJ
american_scientific_journal-2511	87	24	co	co	NOUN
american_scientific_journal-2511	87	25	-	-	PROPN
american_scientific_journal-2511	87	26	i	i	PRON
american_scientific_journal-2511	87	27	genes	gene	NOUN
american_scientific_journal-2511	87	28	were	be	AUX
american_scientific_journal-2511	87	29	purified	purify	VERB
american_scientific_journal-2511	87	30	from	from	ADP
american_scientific_journal-2511	87	31	agarose	agarose	NOUN
american_scientific_journal-2511	87	32	gel	gel	NOUN
american_scientific_journal-2511	87	33	and	and	CCONJ
american_scientific_journal-2511	87	34	analyzed	analyze	VERB
american_scientific_journal-2511	87	35	by	by	ADP
american_scientific_journal-2511	87	36	agarose	agarose	NOUN
american_scientific_journal-2511	87	37	gel	gel	NOUN
american_scientific_journal-2511	87	38	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	87	39	as	as	SCONJ
american_scientific_journal-2511	87	40	shown	show	VERB
american_scientific_journal-2511	87	41	below	below	ADP
american_scientific_journal-2511	87	42	in	in	ADP
american_scientific_journal-2511	87	43	figures	figure	NOUN
american_scientific_journal-2511	87	44	2	2	NUM
american_scientific_journal-2511	87	45	and	and	CCONJ
american_scientific_journal-2511	87	46	3	3	NUM
american_scientific_journal-2511	87	47	for	for	ADP
american_scientific_journal-2511	87	48	18srdna	18srdna	PROPN
american_scientific_journal-2511	87	49	and	and	CCONJ
american_scientific_journal-2511	87	50	co	co	NOUN
american_scientific_journal-2511	87	51	-	-	NOUN
american_scientific_journal-2511	87	52	i	i	PRON
american_scientific_journal-2511	87	53	genes	gene	NOUN
american_scientific_journal-2511	87	54	respectively	respectively	ADV
american_scientific_journal-2511	87	55	.	.	PUNCT
american_scientific_journal-2511	88	1	figure	figure	VERB
american_scientific_journal-2511	88	2	1	1	NUM
american_scientific_journal-2511	88	3	:	:	PUNCT
american_scientific_journal-2511	88	4	the	the	DET
american_scientific_journal-2511	88	5	analysis	analysis	NOUN
american_scientific_journal-2511	88	6	of	of	ADP
american_scientific_journal-2511	88	7	2	2	NUM
american_scientific_journal-2511	88	8	%	%	NOUN
american_scientific_journal-2511	88	9	agarose	agarose	NOUN
american_scientific_journal-2511	88	10	gel	gel	NOUN
american_scientific_journal-2511	88	11	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	88	12	for	for	ADP
american_scientific_journal-2511	88	13	purified	purified	ADJ
american_scientific_journal-2511	88	14	18s	18	NOUN
american_scientific_journal-2511	88	15	rdna	rdna	NOUN
american_scientific_journal-2511	88	16	gene	gene	NOUN
american_scientific_journal-2511	88	17	.	.	PUNCT
american_scientific_journal-2511	89	1	m	m	PROPN
american_scientific_journal-2511	89	2	100bp	100bp	ADJ
american_scientific_journal-2511	89	3	.	.	PUNCT
american_scientific_journal-2511	90	1	dna	dna	PROPN
american_scientific_journal-2511	90	2	marker	marker	NOUN
american_scientific_journal-2511	90	3	,	,	PUNCT
american_scientific_journal-2511	90	4	lanes	lane	VERB
american_scientific_journal-2511	90	5	1	1	NUM
american_scientific_journal-2511	90	6	and	and	CCONJ
american_scientific_journal-2511	90	7	2	2	NUM
american_scientific_journal-2511	90	8	:	:	PUNCT
american_scientific_journal-2511	90	9	18s	18s	NUM
american_scientific_journal-2511	90	10	rdna	rdna	NOUN
american_scientific_journal-2511	90	11	gene	gene	NOUN
american_scientific_journal-2511	90	12	(	(	PUNCT
american_scientific_journal-2511	90	13	1800	1800	NUM
american_scientific_journal-2511	90	14	bp	bp	NOUN
american_scientific_journal-2511	90	15	)	)	PUNCT
american_scientific_journal-2511	90	16	figure	figure	NOUN
american_scientific_journal-2511	90	17	2	2	NUM
american_scientific_journal-2511	90	18	:	:	PUNCT
american_scientific_journal-2511	90	19	the	the	DET
american_scientific_journal-2511	90	20	analysis	analysis	NOUN
american_scientific_journal-2511	90	21	of	of	ADP
american_scientific_journal-2511	90	22	2	2	NUM
american_scientific_journal-2511	90	23	%	%	NOUN
american_scientific_journal-2511	90	24	agarose	agarose	NOUN
american_scientific_journal-2511	90	25	gel	gel	NOUN
american_scientific_journal-2511	90	26	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	90	27	for	for	ADP
american_scientific_journal-2511	90	28	purified	purified	ADJ
american_scientific_journal-2511	90	29	co	co	NOUN
american_scientific_journal-2511	90	30	-	-	PROPN
american_scientific_journal-2511	90	31	i	i	PRON
american_scientific_journal-2511	90	32	gene	gene	NOUN
american_scientific_journal-2511	90	33	.	.	PUNCT
american_scientific_journal-2511	91	1	m	m	VERB
american_scientific_journal-2511	91	2	100bp	100bp	ADJ
american_scientific_journal-2511	91	3	.	.	PUNCT
american_scientific_journal-2511	92	1	dna	dna	PROPN
american_scientific_journal-2511	92	2	marker	marker	NOUN
american_scientific_journal-2511	92	3	,	,	PUNCT
american_scientific_journal-2511	92	4	lanes	lane	VERB
american_scientific_journal-2511	92	5	1	1	NUM
american_scientific_journal-2511	92	6	:	:	PUNCT
american_scientific_journal-2511	92	7	co	co	NOUN
american_scientific_journal-2511	92	8	-	-	PROPN
american_scientific_journal-2511	92	9	i	i	PRON
american_scientific_journal-2511	92	10	gene	gene	NOUN
american_scientific_journal-2511	92	11	(	(	PUNCT
american_scientific_journal-2511	92	12	659	659	NUM
american_scientific_journal-2511	92	13	bp	bp	NOUN
american_scientific_journal-2511	92	14	)	)	PUNCT
american_scientific_journal-2511	92	15	.	.	PUNCT
american_scientific_journal-2511	93	1	american	american	PROPN
american_scientific_journal-2511	93	2	scientific	scientific	ADJ
american_scientific_journal-2511	93	3	research	research	PROPN
american_scientific_journal-2511	93	4	journal	journal	NOUN
american_scientific_journal-2511	93	5	for	for	ADP
american_scientific_journal-2511	93	6	engineering	engineering	NOUN
american_scientific_journal-2511	93	7	,	,	PUNCT
american_scientific_journal-2511	93	8	technology	technology	NOUN
american_scientific_journal-2511	93	9	,	,	PUNCT
american_scientific_journal-2511	93	10	and	and	CCONJ
american_scientific_journal-2511	93	11	sciences	science	NOUN
american_scientific_journal-2511	93	12	(	(	PUNCT
american_scientific_journal-2511	93	13	asrjets	asrjet	NOUN
american_scientific_journal-2511	93	14	)	)	PUNCT
american_scientific_journal-2511	93	15	(	(	PUNCT
american_scientific_journal-2511	93	16	2017	2017	NUM
american_scientific_journal-2511	93	17	)	)	PUNCT
american_scientific_journal-2511	93	18	volume	volume	NOUN
american_scientific_journal-2511	93	19	27	27	NUM
american_scientific_journal-2511	93	20	,	,	PUNCT
american_scientific_journal-2511	93	21	no	no	DET
american_scientific_journal-2511	93	22	1	1	NUM
american_scientific_journal-2511	93	23	,	,	PUNCT
american_scientific_journal-2511	93	24	pp	pp	ADV
american_scientific_journal-2511	93	25	25	25	NUM
american_scientific_journal-2511	93	26	-	-	SYM
american_scientific_journal-2511	93	27	42	42	NUM
american_scientific_journal-2511	93	28	31	31	NUM
american_scientific_journal-2511	93	29	18s	18	NOUN
american_scientific_journal-2511	93	30	rdna	rdna	PROPN
american_scientific_journal-2511	93	31	and	and	CCONJ
american_scientific_journal-2511	93	32	co	co	NOUN
american_scientific_journal-2511	93	33	-	-	PROPN
american_scientific_journal-2511	93	34	i	i	PRON
american_scientific_journal-2511	93	35	genes	gene	NOUN
american_scientific_journal-2511	93	36	were	be	AUX
american_scientific_journal-2511	93	37	successfully	successfully	ADV
american_scientific_journal-2511	93	38	sequenced	sequence	VERB
american_scientific_journal-2511	93	39	,	,	PUNCT
american_scientific_journal-2511	93	40	aligned	align	VERB
american_scientific_journal-2511	93	41	with	with	ADP
american_scientific_journal-2511	93	42	blast	blast	NOUN
american_scientific_journal-2511	93	43	,	,	PUNCT
american_scientific_journal-2511	93	44	and	and	CCONJ
american_scientific_journal-2511	93	45	leeches	leech	NOUN
american_scientific_journal-2511	93	46	were	be	AUX
american_scientific_journal-2511	93	47	identified	identify	VERB
american_scientific_journal-2511	93	48	as	as	ADP
american_scientific_journal-2511	93	49	h.	h.	NOUN
american_scientific_journal-2511	93	50	orientalis	orientalis	PROPN
american_scientific_journal-2511	93	51	.	.	PUNCT
american_scientific_journal-2511	94	1	3.2	3.2	NUM
american_scientific_journal-2511	94	2	extraction	extraction	NOUN
american_scientific_journal-2511	94	3	of	of	ADP
american_scientific_journal-2511	94	4	total	total	ADJ
american_scientific_journal-2511	94	5	rna	rna	NOUN
american_scientific_journal-2511	94	6	from	from	ADP
american_scientific_journal-2511	94	7	medical	medical	ADJ
american_scientific_journal-2511	94	8	leeches	leech	NOUN
american_scientific_journal-2511	94	9	agarose	agarose	NOUN
american_scientific_journal-2511	94	10	gel	gel	NOUN
american_scientific_journal-2511	94	11	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	94	12	was	be	AUX
american_scientific_journal-2511	94	13	performed	perform	VERB
american_scientific_journal-2511	94	14	to	to	PART
american_scientific_journal-2511	94	15	detect	detect	VERB
american_scientific_journal-2511	94	16	the	the	DET
american_scientific_journal-2511	94	17	purified	purified	ADJ
american_scientific_journal-2511	94	18	total	total	ADJ
american_scientific_journal-2511	94	19	rna	rna	NOUN
american_scientific_journal-2511	94	20	from	from	ADP
american_scientific_journal-2511	94	21	medical	medical	ADJ
american_scientific_journal-2511	94	22	leeches	leech	NOUN
american_scientific_journal-2511	94	23	figure	figure	VERB
american_scientific_journal-2511	94	24	3	3	NUM
american_scientific_journal-2511	94	25	.	.	PUNCT
american_scientific_journal-2511	94	26	with	with	ADP
american_scientific_journal-2511	94	27	concentration	concentration	NOUN
american_scientific_journal-2511	94	28	about	about	ADV
american_scientific_journal-2511	94	29	210	210	NUM
american_scientific_journal-2511	94	30	µg	µg	ADV
american_scientific_journal-2511	94	31	in	in	ADP
american_scientific_journal-2511	94	32	total	total	ADJ
american_scientific_journal-2511	94	33	volume	volume	NOUN
american_scientific_journal-2511	94	34	35µl	35µl	NOUN
american_scientific_journal-2511	94	35	.	.	PUNCT
american_scientific_journal-2511	95	1	figure	figure	NOUN
american_scientific_journal-2511	95	2	3	3	NUM
american_scientific_journal-2511	95	3	:	:	PUNCT
american_scientific_journal-2511	95	4	the	the	DET
american_scientific_journal-2511	95	5	analysis	analysis	NOUN
american_scientific_journal-2511	95	6	of	of	ADP
american_scientific_journal-2511	95	7	0.8	0.8	NUM
american_scientific_journal-2511	95	8	%	%	NOUN
american_scientific_journal-2511	95	9	agarose	agarose	NOUN
american_scientific_journal-2511	95	10	gel	gel	NOUN
american_scientific_journal-2511	95	11	electrophoresis	electrophoresis	PROPN
american_scientific_journal-2511	95	12	total	total	NOUN
american_scientific_journal-2511	95	13	rna	rna	NOUN
american_scientific_journal-2511	95	14	purified	purify	VERB
american_scientific_journal-2511	95	15	from	from	ADP
american_scientific_journal-2511	95	16	medical	medical	ADJ
american_scientific_journal-2511	95	17	leeches	leech	NOUN
american_scientific_journal-2511	95	18	.	.	PUNCT
american_scientific_journal-2511	96	1	m	m	VERB
american_scientific_journal-2511	96	2	500bp	500bp	NOUN
american_scientific_journal-2511	96	3	.	.	PUNCT
american_scientific_journal-2511	97	1	dna	dna	PROPN
american_scientific_journal-2511	97	2	marker	marker	NOUN
american_scientific_journal-2511	97	3	,	,	PUNCT
american_scientific_journal-2511	97	4	lanes	lane	VERB
american_scientific_journal-2511	97	5	1	1	NUM
american_scientific_journal-2511	97	6	:	:	PUNCT
american_scientific_journal-2511	97	7	total	total	ADJ
american_scientific_journal-2511	97	8	rna	rna	NOUN
american_scientific_journal-2511	97	9	sample	sample	NOUN
american_scientific_journal-2511	97	10	3.3	3.3	NUM
american_scientific_journal-2511	97	11	reverse	reverse	NOUN
american_scientific_journal-2511	97	12	transcriptase	transcriptase	NOUN
american_scientific_journal-2511	97	13	cdna	cdna	PROPN
american_scientific_journal-2511	97	14	synthesis	synthesis	NOUN
american_scientific_journal-2511	97	15	figure	figure	NOUN
american_scientific_journal-2511	97	16	4	4	NUM
american_scientific_journal-2511	97	17	shows	show	VERB
american_scientific_journal-2511	97	18	the	the	DET
american_scientific_journal-2511	97	19	cdna	cdna	PROPN
american_scientific_journal-2511	97	20	band	band	NOUN
american_scientific_journal-2511	97	21	on	on	ADP
american_scientific_journal-2511	97	22	0.8	0.8	NUM
american_scientific_journal-2511	97	23	%	%	NOUN
american_scientific_journal-2511	97	24	agarose	agarose	NOUN
american_scientific_journal-2511	97	25	gel	gel	NOUN
american_scientific_journal-2511	97	26	containing	contain	VERB
american_scientific_journal-2511	97	27	1	1	NUM
american_scientific_journal-2511	97	28	%	%	NOUN
american_scientific_journal-2511	97	29	ethidium	ethidium	NOUN
american_scientific_journal-2511	97	30	bromide	bromide	NOUN
american_scientific_journal-2511	97	31	.	.	PUNCT
american_scientific_journal-2511	98	1	figure	figure	VERB
american_scientific_journal-2511	98	2	4	4	NUM
american_scientific_journal-2511	98	3	:	:	PUNCT
american_scientific_journal-2511	98	4	the	the	DET
american_scientific_journal-2511	98	5	analysis	analysis	NOUN
american_scientific_journal-2511	98	6	of	of	ADP
american_scientific_journal-2511	98	7	0.8	0.8	NUM
american_scientific_journal-2511	98	8	%	%	NOUN
american_scientific_journal-2511	98	9	agarose	agarose	NOUN
american_scientific_journal-2511	98	10	gel	gel	NOUN
american_scientific_journal-2511	98	11	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	98	12	for	for	ADP
american_scientific_journal-2511	98	13	cdna	cdna	PROPN
american_scientific_journal-2511	98	14	synthesis	synthesis	NOUN
american_scientific_journal-2511	98	15	by	by	ADP
american_scientific_journal-2511	98	16	rt	rt	PROPN
american_scientific_journal-2511	98	17	process	process	NOUN
american_scientific_journal-2511	98	18	m	m	PROPN
american_scientific_journal-2511	98	19	100bp	100bp	ADJ
american_scientific_journal-2511	98	20	.	.	PUNCT
american_scientific_journal-2511	99	1	dna	dna	PROPN
american_scientific_journal-2511	99	2	marker	marker	NOUN
american_scientific_journal-2511	99	3	,	,	PUNCT
american_scientific_journal-2511	99	4	lanes	lane	NOUN
american_scientific_journal-2511	99	5	1	1	NUM
american_scientific_journal-2511	99	6	:	:	PUNCT
american_scientific_journal-2511	99	7	synthesized	synthesize	VERB
american_scientific_journal-2511	99	8	cdna	cdna	PROPN
american_scientific_journal-2511	99	9	.	.	PUNCT
american_scientific_journal-2511	100	1	american	american	PROPN
american_scientific_journal-2511	100	2	scientific	scientific	ADJ
american_scientific_journal-2511	100	3	research	research	PROPN
american_scientific_journal-2511	100	4	journal	journal	NOUN
american_scientific_journal-2511	100	5	for	for	ADP
american_scientific_journal-2511	100	6	engineering	engineering	NOUN
american_scientific_journal-2511	100	7	,	,	PUNCT
american_scientific_journal-2511	100	8	technology	technology	NOUN
american_scientific_journal-2511	100	9	,	,	PUNCT
american_scientific_journal-2511	100	10	and	and	CCONJ
american_scientific_journal-2511	100	11	sciences	science	NOUN
american_scientific_journal-2511	100	12	(	(	PUNCT
american_scientific_journal-2511	100	13	asrjets	asrjet	NOUN
american_scientific_journal-2511	100	14	)	)	PUNCT
american_scientific_journal-2511	100	15	(	(	PUNCT
american_scientific_journal-2511	100	16	2017	2017	NUM
american_scientific_journal-2511	100	17	)	)	PUNCT
american_scientific_journal-2511	100	18	volume	volume	NOUN
american_scientific_journal-2511	100	19	27	27	NUM
american_scientific_journal-2511	100	20	,	,	PUNCT
american_scientific_journal-2511	100	21	no	no	DET
american_scientific_journal-2511	100	22	1	1	NUM
american_scientific_journal-2511	100	23	,	,	PUNCT
american_scientific_journal-2511	100	24	pp	pp	ADV
american_scientific_journal-2511	100	25	25	25	NUM
american_scientific_journal-2511	100	26	-	-	SYM
american_scientific_journal-2511	100	27	42	42	NUM
american_scientific_journal-2511	100	28	32	32	NUM
american_scientific_journal-2511	100	29	3.4	3.4	NUM
american_scientific_journal-2511	100	30	polymerase	polymerase	NOUN
american_scientific_journal-2511	100	31	chain	chain	NOUN
american_scientific_journal-2511	100	32	reaction	reaction	NOUN
american_scientific_journal-2511	100	33	(	(	PUNCT
american_scientific_journal-2511	100	34	pcr	pcr	NOUN
american_scientific_journal-2511	100	35	)	)	PUNCT
american_scientific_journal-2511	100	36	amplification	amplification	NOUN
american_scientific_journal-2511	100	37	of	of	ADP
american_scientific_journal-2511	100	38	hirudin	hirudin	VERB
american_scientific_journal-2511	100	39	gene	gene	NOUN
american_scientific_journal-2511	100	40	the	the	DET
american_scientific_journal-2511	100	41	hirudin	hirudin	VERB
american_scientific_journal-2511	100	42	gene	gene	NOUN
american_scientific_journal-2511	100	43	was	be	AUX
american_scientific_journal-2511	100	44	amplified	amplify	VERB
american_scientific_journal-2511	100	45	from	from	ADP
american_scientific_journal-2511	100	46	leeches	leech	NOUN
american_scientific_journal-2511	100	47	’	'	PUNCT
american_scientific_journal-2511	100	48	cdna	cdna	PROPN
american_scientific_journal-2511	100	49	,	,	PUNCT
american_scientific_journal-2511	100	50	then	then	ADV
american_scientific_journal-2511	100	51	subjected	subject	VERB
american_scientific_journal-2511	100	52	to	to	ADP
american_scientific_journal-2511	100	53	gel	gel	NOUN
american_scientific_journal-2511	100	54	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	100	55	analysis	analysis	NOUN
american_scientific_journal-2511	100	56	figure	figure	NOUN
american_scientific_journal-2511	100	57	5	5	NUM
american_scientific_journal-2511	100	58	,	,	PUNCT
american_scientific_journal-2511	100	59	the	the	DET
american_scientific_journal-2511	100	60	amplified	amplify	VERB
american_scientific_journal-2511	100	61	individual	individual	ADJ
american_scientific_journal-2511	100	62	gene	gene	NOUN
american_scientific_journal-2511	100	63	band	band	NOUN
american_scientific_journal-2511	100	64	about	about	ADP
american_scientific_journal-2511	100	65	255bp	255bp	NOUN
american_scientific_journal-2511	100	66	with	with	ADP
american_scientific_journal-2511	100	67	a´-overhang	a´-overhang	PROPN
american_scientific_journal-2511	100	68	.	.	PUNCT
american_scientific_journal-2511	101	1	figure	figure	NOUN
american_scientific_journal-2511	101	2	5	5	NUM
american_scientific_journal-2511	101	3	:	:	PUNCT
american_scientific_journal-2511	101	4	the	the	DET
american_scientific_journal-2511	101	5	analysis	analysis	NOUN
american_scientific_journal-2511	101	6	of	of	ADP
american_scientific_journal-2511	101	7	2	2	NUM
american_scientific_journal-2511	101	8	%	%	NOUN
american_scientific_journal-2511	101	9	agarose	agarose	NOUN
american_scientific_journal-2511	101	10	gel	gel	NOUN
american_scientific_journal-2511	101	11	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	101	12	for	for	ADP
american_scientific_journal-2511	101	13	purified	purified	ADJ
american_scientific_journal-2511	101	14	hirudin	hirudin	VERB
american_scientific_journal-2511	101	15	gene	gene	NOUN
american_scientific_journal-2511	101	16	(	(	PUNCT
american_scientific_journal-2511	101	17	255	255	NUM
american_scientific_journal-2511	101	18	bp	bp	PROPN
american_scientific_journal-2511	101	19	)	)	PUNCT
american_scientific_journal-2511	102	1	m	m	VERB
american_scientific_journal-2511	102	2	100bp	100bp	ADJ
american_scientific_journal-2511	102	3	.	.	PUNCT
american_scientific_journal-2511	103	1	dna	dna	PROPN
american_scientific_journal-2511	103	2	marker	marker	NOUN
american_scientific_journal-2511	103	3	,	,	PUNCT
american_scientific_journal-2511	103	4	lanes	lane	VERB
american_scientific_journal-2511	103	5	1	1	NUM
american_scientific_journal-2511	103	6	and	and	CCONJ
american_scientific_journal-2511	103	7	2	2	NUM
american_scientific_journal-2511	103	8	:	:	PUNCT
american_scientific_journal-2511	103	9	hirudin	hirudin	VERB
american_scientific_journal-2511	103	10	gene	gene	NOUN
american_scientific_journal-2511	103	11	.	.	PUNCT
american_scientific_journal-2511	104	1	3.5	3.5	NUM
american_scientific_journal-2511	104	2	transformation	transformation	NOUN
american_scientific_journal-2511	104	3	of	of	ADP
american_scientific_journal-2511	104	4	competent	competent	ADJ
american_scientific_journal-2511	104	5	cells	cell	NOUN
american_scientific_journal-2511	104	6	(	(	PUNCT
american_scientific_journal-2511	104	7	a	a	X
american_scientific_journal-2511	104	8	)	)	PUNCT
american_scientific_journal-2511	104	9	(	(	PUNCT
american_scientific_journal-2511	104	10	b	b	X
american_scientific_journal-2511	104	11	)	)	PUNCT
american_scientific_journal-2511	104	12	figure	figure	NOUN
american_scientific_journal-2511	104	13	6	6	NUM
american_scientific_journal-2511	104	14	:	:	PUNCT
american_scientific_journal-2511	104	15	the	the	DET
american_scientific_journal-2511	104	16	transformed	transform	VERB
american_scientific_journal-2511	104	17	cells	cell	NOUN
american_scientific_journal-2511	104	18	.	.	PUNCT
american_scientific_journal-2511	105	1	(	(	PUNCT
american_scientific_journal-2511	105	2	a	a	X
american_scientific_journal-2511	105	3	):	):	PUNCT
american_scientific_journal-2511	105	4	dh5α	dh5α	PROPN
american_scientific_journal-2511	105	5	and	and	CCONJ
american_scientific_journal-2511	105	6	(	(	PUNCT
american_scientific_journal-2511	105	7	b	b	NOUN
american_scientific_journal-2511	105	8	):	):	PUNCT
american_scientific_journal-2511	105	9	bl21(de3	bl21(de3	VERB
american_scientific_journal-2511	105	10	)	)	PUNCT
american_scientific_journal-2511	105	11	american	american	ADJ
american_scientific_journal-2511	105	12	scientific	scientific	ADJ
american_scientific_journal-2511	105	13	research	research	NOUN
american_scientific_journal-2511	105	14	journal	journal	NOUN
american_scientific_journal-2511	105	15	for	for	ADP
american_scientific_journal-2511	105	16	engineering	engineering	NOUN
american_scientific_journal-2511	105	17	,	,	PUNCT
american_scientific_journal-2511	105	18	technology	technology	NOUN
american_scientific_journal-2511	105	19	,	,	PUNCT
american_scientific_journal-2511	105	20	and	and	CCONJ
american_scientific_journal-2511	105	21	sciences	science	NOUN
american_scientific_journal-2511	105	22	(	(	PUNCT
american_scientific_journal-2511	105	23	asrjets	asrjet	NOUN
american_scientific_journal-2511	105	24	)	)	PUNCT
american_scientific_journal-2511	105	25	(	(	PUNCT
american_scientific_journal-2511	105	26	2017	2017	NUM
american_scientific_journal-2511	105	27	)	)	PUNCT
american_scientific_journal-2511	105	28	volume	volume	NOUN
american_scientific_journal-2511	105	29	27	27	NUM
american_scientific_journal-2511	105	30	,	,	PUNCT
american_scientific_journal-2511	105	31	no	no	DET
american_scientific_journal-2511	105	32	1	1	NUM
american_scientific_journal-2511	105	33	,	,	PUNCT
american_scientific_journal-2511	105	34	pp	pp	ADV
american_scientific_journal-2511	105	35	25	25	NUM
american_scientific_journal-2511	105	36	-	-	SYM
american_scientific_journal-2511	105	37	42	42	NUM
american_scientific_journal-2511	105	38	33	33	NUM
american_scientific_journal-2511	105	39	two	two	NUM
american_scientific_journal-2511	105	40	types	type	NOUN
american_scientific_journal-2511	105	41	of	of	ADP
american_scientific_journal-2511	105	42	competent	competent	ADJ
american_scientific_journal-2511	105	43	genetic	genetic	ADJ
american_scientific_journal-2511	105	44	engineering	engineering	NOUN
american_scientific_journal-2511	105	45	cells	cell	NOUN
american_scientific_journal-2511	105	46	were	be	AUX
american_scientific_journal-2511	105	47	transformed	transform	VERB
american_scientific_journal-2511	105	48	with	with	ADP
american_scientific_journal-2511	105	49	vector	vector	NOUN
american_scientific_journal-2511	105	50	pet-16b	pet-16b	PROPN
american_scientific_journal-2511	105	51	-	-	PUNCT
american_scientific_journal-2511	105	52	hr	hr	NOUN
american_scientific_journal-2511	105	53	,	,	PUNCT
american_scientific_journal-2511	105	54	the	the	DET
american_scientific_journal-2511	105	55	results	result	NOUN
american_scientific_journal-2511	105	56	in	in	ADP
american_scientific_journal-2511	105	57	figure	figure	NOUN
american_scientific_journal-2511	105	58	6	6	NUM
american_scientific_journal-2511	105	59	(	(	PUNCT
american_scientific_journal-2511	105	60	a&b	a&b	PROPN
american_scientific_journal-2511	105	61	)	)	PUNCT
american_scientific_journal-2511	105	62	,	,	PUNCT
american_scientific_journal-2511	105	63	show	show	VERB
american_scientific_journal-2511	105	64	the	the	DET
american_scientific_journal-2511	105	65	transformed	transform	VERB
american_scientific_journal-2511	105	66	cells	cell	NOUN
american_scientific_journal-2511	105	67	of	of	ADP
american_scientific_journal-2511	105	68	dh5α	dh5α	PROPN
american_scientific_journal-2511	105	69	and	and	CCONJ
american_scientific_journal-2511	105	70	bl21(de3	bl21(de3	VERB
american_scientific_journal-2511	105	71	)	)	PUNCT
american_scientific_journal-2511	105	72	respectively	respectively	ADV
american_scientific_journal-2511	105	73	.	.	PUNCT
american_scientific_journal-2511	106	1	3.6	3.6	NUM
american_scientific_journal-2511	106	2	screening	screen	VERB
american_scientific_journal-2511	106	3	by	by	ADP
american_scientific_journal-2511	106	4	extraction	extraction	NOUN
american_scientific_journal-2511	106	5	pet-16	pet-16	NOUN
american_scientific_journal-2511	106	6	-	-	ADJ
american_scientific_journal-2511	106	7	hr	hr	ADJ
american_scientific_journal-2511	106	8	(	(	PUNCT
american_scientific_journal-2511	106	9	constructive	constructive	ADJ
american_scientific_journal-2511	106	10	vector	vector	NOUN
american_scientific_journal-2511	106	11	)	)	PUNCT
american_scientific_journal-2511	106	12	figure	figure	NOUN
american_scientific_journal-2511	106	13	7	7	NUM
american_scientific_journal-2511	106	14	shows	show	VERB
american_scientific_journal-2511	106	15	the	the	DET
american_scientific_journal-2511	106	16	band	band	NOUN
american_scientific_journal-2511	106	17	of	of	ADP
american_scientific_journal-2511	106	18	constructive	constructive	ADJ
american_scientific_journal-2511	106	19	plasmid	plasmid	NOUN
american_scientific_journal-2511	106	20	of	of	ADP
american_scientific_journal-2511	106	21	about	about	ADP
american_scientific_journal-2511	106	22	(	(	PUNCT
american_scientific_journal-2511	106	23	5966bp	5966bp	ADJ
american_scientific_journal-2511	106	24	)	)	PUNCT
american_scientific_journal-2511	106	25	after	after	ADP
american_scientific_journal-2511	106	26	extraction	extraction	NOUN
american_scientific_journal-2511	106	27	it	it	PRON
american_scientific_journal-2511	106	28	from	from	ADP
american_scientific_journal-2511	106	29	competent	competent	ADJ
american_scientific_journal-2511	106	30	cells	cell	NOUN
american_scientific_journal-2511	106	31	,	,	PUNCT
american_scientific_journal-2511	106	32	also	also	ADV
american_scientific_journal-2511	106	33	,	,	PUNCT
american_scientific_journal-2511	106	34	figure	figure	VERB
american_scientific_journal-2511	106	35	8	8	NUM
american_scientific_journal-2511	106	36	shows	show	VERB
american_scientific_journal-2511	106	37	the	the	DET
american_scientific_journal-2511	106	38	band	band	NOUN
american_scientific_journal-2511	106	39	of	of	ADP
american_scientific_journal-2511	106	40	target	target	NOUN
american_scientific_journal-2511	106	41	gene	gene	NOUN
american_scientific_journal-2511	106	42	hirudin	hirudin	VERB
american_scientific_journal-2511	106	43	255bp	255bp	NOUN
american_scientific_journal-2511	106	44	after	after	SCONJ
american_scientific_journal-2511	106	45	amplified	amplify	VERB
american_scientific_journal-2511	106	46	it	it	PRON
american_scientific_journal-2511	106	47	from	from	ADP
american_scientific_journal-2511	106	48	extraction	extraction	NOUN
american_scientific_journal-2511	106	49	constructive	constructive	ADJ
american_scientific_journal-2511	106	50	plasmid	plasmid	NOUN
american_scientific_journal-2511	106	51	by	by	ADP
american_scientific_journal-2511	106	52	(	(	PUNCT
american_scientific_journal-2511	106	53	hr1	hr1	PROPN
american_scientific_journal-2511	106	54	,	,	PUNCT
american_scientific_journal-2511	106	55	hr2	hr2	PROPN
american_scientific_journal-2511	106	56	primers	primer	NOUN
american_scientific_journal-2511	106	57	)	)	PUNCT
american_scientific_journal-2511	106	58	.	.	PUNCT
american_scientific_journal-2511	107	1	figure	figure	VERB
american_scientific_journal-2511	107	2	7	7	NUM
american_scientific_journal-2511	107	3	:	:	PUNCT
american_scientific_journal-2511	107	4	the	the	DET
american_scientific_journal-2511	107	5	analysis	analysis	NOUN
american_scientific_journal-2511	107	6	of	of	ADP
american_scientific_journal-2511	107	7	0.8	0.8	NUM
american_scientific_journal-2511	107	8	%	%	NOUN
american_scientific_journal-2511	107	9	agarose	agarose	NOUN
american_scientific_journal-2511	107	10	gel	gel	NOUN
american_scientific_journal-2511	107	11	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	107	12	for	for	ADP
american_scientific_journal-2511	107	13	constructive	constructive	ADJ
american_scientific_journal-2511	107	14	pet-16b	pet-16b	PROPN
american_scientific_journal-2511	107	15	-	-	PUNCT
american_scientific_journal-2511	107	16	hr	hr	NOUN
american_scientific_journal-2511	107	17	extracted	extract	VERB
american_scientific_journal-2511	107	18	from	from	ADP
american_scientific_journal-2511	107	19	competent	competent	ADJ
american_scientific_journal-2511	107	20	cells	cell	NOUN
american_scientific_journal-2511	107	21	.	.	PUNCT
american_scientific_journal-2511	108	1	m	m	VERB
american_scientific_journal-2511	108	2	:	:	PUNCT
american_scientific_journal-2511	108	3	500	500	NUM
american_scientific_journal-2511	108	4	bp	bp	PROPN
american_scientific_journal-2511	108	5	dna	dna	PROPN
american_scientific_journal-2511	108	6	marker	marker	NOUN
american_scientific_journal-2511	108	7	,	,	PUNCT
american_scientific_journal-2511	108	8	lane	lane	NOUN
american_scientific_journal-2511	108	9	1	1	NUM
american_scientific_journal-2511	108	10	:	:	PUNCT
american_scientific_journal-2511	108	11	control	control	NOUN
american_scientific_journal-2511	108	12	with	with	ADP
american_scientific_journal-2511	108	13	5711bp	5711bp	NOUN
american_scientific_journal-2511	108	14	and	and	CCONJ
american_scientific_journal-2511	108	15	lane	lane	NOUN
american_scientific_journal-2511	108	16	2	2	NUM
american_scientific_journal-2511	108	17	:	:	PUNCT
american_scientific_journal-2511	108	18	constructive	constructive	ADJ
american_scientific_journal-2511	108	19	vector	vector	NOUN
american_scientific_journal-2511	108	20	pet-16bhr	pet-16bhr	X
american_scientific_journal-2511	108	21	(	(	PUNCT
american_scientific_journal-2511	108	22	5966bp	5966bp	ADJ
american_scientific_journal-2511	108	23	)	)	PUNCT
american_scientific_journal-2511	108	24	figure	figure	NOUN
american_scientific_journal-2511	108	25	8	8	NUM
american_scientific_journal-2511	108	26	:	:	PUNCT
american_scientific_journal-2511	108	27	analysis	analysis	NOUN
american_scientific_journal-2511	108	28	of	of	ADP
american_scientific_journal-2511	108	29	2	2	NUM
american_scientific_journal-2511	108	30	%	%	NOUN
american_scientific_journal-2511	108	31	agarose	agarose	NOUN
american_scientific_journal-2511	108	32	gel	gel	NOUN
american_scientific_journal-2511	108	33	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	108	34	for	for	ADP
american_scientific_journal-2511	108	35	hirudin	hirudin	VERB
american_scientific_journal-2511	108	36	gene	gene	NOUN
american_scientific_journal-2511	108	37	amplified	amplify	VERB
american_scientific_journal-2511	108	38	from	from	ADP
american_scientific_journal-2511	108	39	constructive	constructive	ADJ
american_scientific_journal-2511	108	40	vector	vector	NOUN
american_scientific_journal-2511	108	41	pet16b	pet16b	NOUN
american_scientific_journal-2511	108	42	-	-	PUNCT
american_scientific_journal-2511	108	43	hr	hr	NOUN
american_scientific_journal-2511	108	44	after	after	SCONJ
american_scientific_journal-2511	108	45	extracted	extract	VERB
american_scientific_journal-2511	108	46	from	from	ADP
american_scientific_journal-2511	108	47	competent	competent	ADJ
american_scientific_journal-2511	108	48	cells	cell	NOUN
american_scientific_journal-2511	108	49	.	.	PUNCT
american_scientific_journal-2511	109	1	m	m	VERB
american_scientific_journal-2511	109	2	:	:	PUNCT
american_scientific_journal-2511	109	3	100bp	100bp	ADJ
american_scientific_journal-2511	109	4	dna	dna	PROPN
american_scientific_journal-2511	109	5	marker	marker	NOUN
american_scientific_journal-2511	109	6	.	.	PUNCT
american_scientific_journal-2511	109	7	,	,	PUNCT
american_scientific_journal-2511	109	8	lanes	lane	VERB
american_scientific_journal-2511	109	9	1	1	NUM
american_scientific_journal-2511	109	10	(	(	PUNCT
american_scientific_journal-2511	109	11	hirudin	hirudin	NOUN
american_scientific_journal-2511	109	12	)	)	PUNCT
american_scientific_journal-2511	109	13	gene	gene	NOUN
american_scientific_journal-2511	109	14	amplifying	amplify	VERB
american_scientific_journal-2511	109	15	from	from	ADP
american_scientific_journal-2511	109	16	cdna	cdna	PROPN
american_scientific_journal-2511	109	17	and	and	CCONJ
american_scientific_journal-2511	109	18	2	2	NUM
american_scientific_journal-2511	109	19	:	:	PUNCT
american_scientific_journal-2511	109	20	(	(	PUNCT
american_scientific_journal-2511	109	21	hirudin	hirudin	VERB
american_scientific_journal-2511	109	22	gene	gene	NOUN
american_scientific_journal-2511	109	23	amplifying	amplify	VERB
american_scientific_journal-2511	109	24	from	from	ADP
american_scientific_journal-2511	109	25	constructive	constructive	ADJ
american_scientific_journal-2511	109	26	vector	vector	NOUN
american_scientific_journal-2511	109	27	pet-16b	pet-16b	NOUN
american_scientific_journal-2511	109	28	-	-	PUNCT
american_scientific_journal-2511	109	29	hirudin	hirudin	NOUN
american_scientific_journal-2511	109	30	)	)	PUNCT
american_scientific_journal-2511	109	31	255bp	255bp	NOUN
american_scientific_journal-2511	109	32	.	.	PUNCT
american_scientific_journal-2511	110	1	2	2	NUM
american_scientific_journal-2511	110	2	1	1	NUM
american_scientific_journal-2511	110	3	m	m	NOUN
american_scientific_journal-2511	110	4	american	american	ADJ
american_scientific_journal-2511	110	5	scientific	scientific	ADJ
american_scientific_journal-2511	110	6	research	research	NOUN
american_scientific_journal-2511	110	7	journal	journal	NOUN
american_scientific_journal-2511	110	8	for	for	ADP
american_scientific_journal-2511	110	9	engineering	engineering	NOUN
american_scientific_journal-2511	110	10	,	,	PUNCT
american_scientific_journal-2511	110	11	technology	technology	NOUN
american_scientific_journal-2511	110	12	,	,	PUNCT
american_scientific_journal-2511	110	13	and	and	CCONJ
american_scientific_journal-2511	110	14	sciences	science	NOUN
american_scientific_journal-2511	110	15	(	(	PUNCT
american_scientific_journal-2511	110	16	asrjets	asrjet	NOUN
american_scientific_journal-2511	110	17	)	)	PUNCT
american_scientific_journal-2511	110	18	(	(	PUNCT
american_scientific_journal-2511	110	19	2017	2017	NUM
american_scientific_journal-2511	110	20	)	)	PUNCT
american_scientific_journal-2511	110	21	volume	volume	NOUN
american_scientific_journal-2511	110	22	27	27	NUM
american_scientific_journal-2511	110	23	,	,	PUNCT
american_scientific_journal-2511	110	24	no	no	DET
american_scientific_journal-2511	110	25	1	1	NUM
american_scientific_journal-2511	110	26	,	,	PUNCT
american_scientific_journal-2511	110	27	pp	pp	ADV
american_scientific_journal-2511	110	28	25	25	NUM
american_scientific_journal-2511	110	29	-	-	SYM
american_scientific_journal-2511	110	30	42	42	NUM
american_scientific_journal-2511	110	31	34	34	NUM
american_scientific_journal-2511	110	32	3.7	3.7	NUM
american_scientific_journal-2511	110	33	fast	fast	ADJ
american_scientific_journal-2511	110	34	screening	screening	NOUN
american_scientific_journal-2511	110	35	of	of	ADP
american_scientific_journal-2511	110	36	hr	hr	NOUN
american_scientific_journal-2511	110	37	gene	gene	NOUN
american_scientific_journal-2511	110	38	by	by	ADP
american_scientific_journal-2511	110	39	digestion	digestion	NOUN
american_scientific_journal-2511	110	40	with	with	ADP
american_scientific_journal-2511	110	41	restriction	restriction	NOUN
american_scientific_journal-2511	110	42	enzyme	enzyme	NOUN
american_scientific_journal-2511	110	43	figure	figure	NOUN
american_scientific_journal-2511	110	44	9	9	NUM
american_scientific_journal-2511	110	45	illustrates	illustrate	VERB
american_scientific_journal-2511	110	46	hirudin	hirudin	VERB
american_scientific_journal-2511	110	47	gene	gene	NOUN
american_scientific_journal-2511	110	48	band	band	NOUN
american_scientific_journal-2511	110	49	about	about	ADP
american_scientific_journal-2511	110	50	(	(	PUNCT
american_scientific_journal-2511	110	51	255bp	255bp	PROPN
american_scientific_journal-2511	110	52	)	)	PUNCT
american_scientific_journal-2511	110	53	after	after	ADP
american_scientific_journal-2511	110	54	digestion	digestion	NOUN
american_scientific_journal-2511	110	55	with	with	ADP
american_scientific_journal-2511	110	56	hindiii	hindiii	NOUN
american_scientific_journal-2511	110	57	and	and	CCONJ
american_scientific_journal-2511	110	58	ndei	ndei	NOUN
american_scientific_journal-2511	110	59	restriction	restriction	NOUN
american_scientific_journal-2511	110	60	enzymes	enzyme	NOUN
american_scientific_journal-2511	110	61	.	.	PUNCT
american_scientific_journal-2511	111	1	figure	figure	VERB
american_scientific_journal-2511	111	2	9	9	NUM
american_scientific_journal-2511	111	3	:	:	PUNCT
american_scientific_journal-2511	111	4	hirudin	hirudin	VERB
american_scientific_journal-2511	111	5	gene	gene	NOUN
american_scientific_journal-2511	111	6	band	band	NOUN
american_scientific_journal-2511	111	7	after	after	ADP
american_scientific_journal-2511	111	8	digestion	digestion	NOUN
american_scientific_journal-2511	111	9	with	with	ADP
american_scientific_journal-2511	111	10	restriction	restriction	NOUN
american_scientific_journal-2511	111	11	enzymes	enzyme	NOUN
american_scientific_journal-2511	111	12	(	(	PUNCT
american_scientific_journal-2511	111	13	hindiii	hindiii	ADJ
american_scientific_journal-2511	111	14	and	and	CCONJ
american_scientific_journal-2511	111	15	bamhi	bamhi	VERB
american_scientific_journal-2511	111	16	)	)	PUNCT
american_scientific_journal-2511	111	17	.	.	PUNCT
american_scientific_journal-2511	112	1	m:100bp	m:100bp	PROPN
american_scientific_journal-2511	112	2	dna	dna	PROPN
american_scientific_journal-2511	112	3	marker	marker	NOUN
american_scientific_journal-2511	112	4	,	,	PUNCT
american_scientific_journal-2511	112	5	lane	lane	NOUN
american_scientific_journal-2511	112	6	1	1	NUM
american_scientific_journal-2511	112	7	:	:	PUNCT
american_scientific_journal-2511	112	8	control	control	NOUN
american_scientific_journal-2511	112	9	lane	lane	NOUN
american_scientific_journal-2511	112	10	2	2	NUM
american_scientific_journal-2511	112	11	:	:	PUNCT
american_scientific_journal-2511	112	12	hirudin	hirudin	VERB
american_scientific_journal-2511	112	13	gene	gene	NOUN
american_scientific_journal-2511	112	14	band	band	NOUN
american_scientific_journal-2511	112	15	255	255	NUM
american_scientific_journal-2511	112	16	bp	bp	PROPN
american_scientific_journal-2511	112	17	.	.	PUNCT
american_scientific_journal-2511	113	1	3.8	3.8	NUM
american_scientific_journal-2511	113	2	hirudin	hirudin	VERB
american_scientific_journal-2511	113	3	gene	gene	NOUN
american_scientific_journal-2511	113	4	expression	expression	NOUN
american_scientific_journal-2511	113	5	detection	detection	NOUN
american_scientific_journal-2511	113	6	by	by	ADP
american_scientific_journal-2511	113	7	real	real	ADJ
american_scientific_journal-2511	113	8	time	time	NOUN
american_scientific_journal-2511	113	9	pcr	pcr	ADJ
american_scientific_journal-2511	113	10	hirudin	hirudin	NOUN
american_scientific_journal-2511	113	11	expression	expression	NOUN
american_scientific_journal-2511	113	12	was	be	AUX
american_scientific_journal-2511	113	13	detected	detect	VERB
american_scientific_journal-2511	113	14	by	by	ADP
american_scientific_journal-2511	113	15	real	real	ADJ
american_scientific_journal-2511	113	16	time	time	NOUN
american_scientific_journal-2511	113	17	pcr	pcr	PROPN
american_scientific_journal-2511	113	18	,	,	PUNCT
american_scientific_journal-2511	113	19	by	by	ADP
american_scientific_journal-2511	113	20	extraction	extraction	NOUN
american_scientific_journal-2511	113	21	total	total	NOUN
american_scientific_journal-2511	113	22	rna	rna	NOUN
american_scientific_journal-2511	113	23	from	from	ADP
american_scientific_journal-2511	113	24	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2511	113	25	)	)	PUNCT
american_scientific_journal-2511	113	26	strain	strain	NOUN
american_scientific_journal-2511	113	27	harboring	harbor	VERB
american_scientific_journal-2511	113	28	constructive	constructive	ADJ
american_scientific_journal-2511	113	29	vector	vector	NOUN
american_scientific_journal-2511	113	30	pet-16b	pet-16b	NOUN
american_scientific_journal-2511	113	31	-	-	NOUN
american_scientific_journal-2511	113	32	hr	hr	NOUN
american_scientific_journal-2511	113	33	with	with	ADP
american_scientific_journal-2511	113	34	concentration	concentration	NOUN
american_scientific_journal-2511	113	35	760µg	760µg	PROPN
american_scientific_journal-2511	113	36	/	/	SYM
american_scientific_journal-2511	113	37	ml	ml	PROPN
american_scientific_journal-2511	113	38	.	.	PUNCT
american_scientific_journal-2511	114	1	then	then	ADV
american_scientific_journal-2511	114	2	cdna	cdna	PROPN
american_scientific_journal-2511	114	3	synthesized	synthesize	VERB
american_scientific_journal-2511	114	4	from	from	ADP
american_scientific_journal-2511	114	5	total	total	ADJ
american_scientific_journal-2511	114	6	rna	rna	NOUN
american_scientific_journal-2511	114	7	for	for	ADP
american_scientific_journal-2511	114	8	real	real	ADJ
american_scientific_journal-2511	114	9	time	time	NOUN
american_scientific_journal-2511	114	10	pcr	pcr	ADJ
american_scientific_journal-2511	114	11	reaction	reaction	NOUN
american_scientific_journal-2511	114	12	,	,	PUNCT
american_scientific_journal-2511	114	13	the	the	DET
american_scientific_journal-2511	114	14	results	result	NOUN
american_scientific_journal-2511	114	15	from	from	ADP
american_scientific_journal-2511	114	16	real	real	ADJ
american_scientific_journal-2511	114	17	time	time	NOUN
american_scientific_journal-2511	114	18	pcr	pcr	PROPN
american_scientific_journal-2511	114	19	illustrate	illustrate	VERB
american_scientific_journal-2511	114	20	in	in	ADP
american_scientific_journal-2511	114	21	figure	figure	NOUN
american_scientific_journal-2511	114	22	10	10	NUM
american_scientific_journal-2511	114	23	which	which	PRON
american_scientific_journal-2511	114	24	is	be	AUX
american_scientific_journal-2511	114	25	threshold	threshold	NOUN
american_scientific_journal-2511	114	26	curve	curve	NOUN
american_scientific_journal-2511	114	27	,	,	PUNCT
american_scientific_journal-2511	114	28	ct	ct	NUM
american_scientific_journal-2511	114	29	value	value	NOUN
american_scientific_journal-2511	114	30	for	for	ADP
american_scientific_journal-2511	114	31	hirudin	hirudin	VERB
american_scientific_journal-2511	114	32	gene	gene	NOUN
american_scientific_journal-2511	114	33	was	be	AUX
american_scientific_journal-2511	114	34	(	(	PUNCT
american_scientific_journal-2511	114	35	22.64	22.64	NUM
american_scientific_journal-2511	114	36	)	)	PUNCT
american_scientific_journal-2511	114	37	,	,	PUNCT
american_scientific_journal-2511	114	38	melting	melt	VERB
american_scientific_journal-2511	114	39	curve	curve	NOUN
american_scientific_journal-2511	114	40	illustrated	illustrate	VERB
american_scientific_journal-2511	114	41	in	in	ADP
american_scientific_journal-2511	114	42	figure	figure	NOUN
american_scientific_journal-2511	114	43	11	11	NUM
american_scientific_journal-2511	114	44	.	.	PUNCT
american_scientific_journal-2511	115	1	figure	figure	VERB
american_scientific_journal-2511	115	2	10	10	NUM
american_scientific_journal-2511	115	3	:	:	PUNCT
american_scientific_journal-2511	115	4	threshold	threshold	NOUN
american_scientific_journal-2511	115	5	curve	curve	NOUN
american_scientific_journal-2511	115	6	for	for	ADP
american_scientific_journal-2511	115	7	real	real	ADJ
american_scientific_journal-2511	115	8	time	time	NOUN
american_scientific_journal-2511	115	9	pcr	pcr	PROPN
american_scientific_journal-2511	115	10	.	.	PUNCT
american_scientific_journal-2511	116	1	american	american	PROPN
american_scientific_journal-2511	116	2	scientific	scientific	ADJ
american_scientific_journal-2511	116	3	research	research	PROPN
american_scientific_journal-2511	116	4	journal	journal	NOUN
american_scientific_journal-2511	116	5	for	for	ADP
american_scientific_journal-2511	116	6	engineering	engineering	NOUN
american_scientific_journal-2511	116	7	,	,	PUNCT
american_scientific_journal-2511	116	8	technology	technology	NOUN
american_scientific_journal-2511	116	9	,	,	PUNCT
american_scientific_journal-2511	116	10	and	and	CCONJ
american_scientific_journal-2511	116	11	sciences	science	NOUN
american_scientific_journal-2511	116	12	(	(	PUNCT
american_scientific_journal-2511	116	13	asrjets	asrjet	NOUN
american_scientific_journal-2511	116	14	)	)	PUNCT
american_scientific_journal-2511	116	15	(	(	PUNCT
american_scientific_journal-2511	116	16	2017	2017	NUM
american_scientific_journal-2511	116	17	)	)	PUNCT
american_scientific_journal-2511	116	18	volume	volume	NOUN
american_scientific_journal-2511	116	19	27	27	NUM
american_scientific_journal-2511	116	20	,	,	PUNCT
american_scientific_journal-2511	116	21	no	no	DET
american_scientific_journal-2511	116	22	1	1	NUM
american_scientific_journal-2511	116	23	,	,	PUNCT
american_scientific_journal-2511	116	24	pp	pp	ADV
american_scientific_journal-2511	116	25	25	25	NUM
american_scientific_journal-2511	116	26	-	-	SYM
american_scientific_journal-2511	116	27	42	42	NUM
american_scientific_journal-2511	116	28	35	35	NUM
american_scientific_journal-2511	116	29	figure	figure	NOUN
american_scientific_journal-2511	116	30	11	11	NUM
american_scientific_journal-2511	116	31	:	:	PUNCT
american_scientific_journal-2511	116	32	melting	melt	VERB
american_scientific_journal-2511	116	33	curve	curve	NOUN
american_scientific_journal-2511	116	34	for	for	ADP
american_scientific_journal-2511	116	35	real	real	ADJ
american_scientific_journal-2511	116	36	time	time	NOUN
american_scientific_journal-2511	116	37	pcr	pcr	PROPN
american_scientific_journal-2511	116	38	.	.	PUNCT
american_scientific_journal-2511	117	1	3.9	3.9	NUM
american_scientific_journal-2511	117	2	purification	purification	NOUN
american_scientific_journal-2511	117	3	by	by	ADP
american_scientific_journal-2511	117	4	imac	imac	NOUN
american_scientific_journal-2511	117	5	and	and	CCONJ
american_scientific_journal-2511	117	6	deae	deae	NOUN
american_scientific_journal-2511	117	7	sepharose	sepharose	VERB
american_scientific_journal-2511	117	8	the	the	DET
american_scientific_journal-2511	117	9	presence	presence	NOUN
american_scientific_journal-2511	117	10	of	of	ADP
american_scientific_journal-2511	117	11	his	his	PRON
american_scientific_journal-2511	117	12	-	-	PUNCT
american_scientific_journal-2511	117	13	tag	tag	NOUN
american_scientific_journal-2511	117	14	in	in	ADP
american_scientific_journal-2511	117	15	the	the	DET
american_scientific_journal-2511	117	16	protein	protein	NOUN
american_scientific_journal-2511	117	17	studied	study	VERB
american_scientific_journal-2511	117	18	in	in	ADP
american_scientific_journal-2511	117	19	this	this	DET
american_scientific_journal-2511	117	20	study	study	NOUN
american_scientific_journal-2511	117	21	lead	lead	NOUN
american_scientific_journal-2511	117	22	to	to	PART
american_scientific_journal-2511	117	23	use	use	VERB
american_scientific_journal-2511	117	24	imac	imac	NOUN
american_scientific_journal-2511	117	25	technique	technique	NOUN
american_scientific_journal-2511	117	26	,	,	PUNCT
american_scientific_journal-2511	117	27	which	which	PRON
american_scientific_journal-2511	117	28	make	make	VERB
american_scientific_journal-2511	117	29	easy	easy	ADJ
american_scientific_journal-2511	117	30	to	to	PART
american_scientific_journal-2511	117	31	elute	elute	VERB
american_scientific_journal-2511	117	32	his	his	PRON
american_scientific_journal-2511	117	33	-	-	PUNCT
american_scientific_journal-2511	117	34	tag	tag	NOUN
american_scientific_journal-2511	117	35	protein	protein	NOUN
american_scientific_journal-2511	117	36	in	in	ADP
american_scientific_journal-2511	117	37	elution	elution	NOUN
american_scientific_journal-2511	117	38	buffer	buffer	NOUN
american_scientific_journal-2511	117	39	but	but	CCONJ
american_scientific_journal-2511	117	40	for	for	ADP
american_scientific_journal-2511	117	41	more	more	ADJ
american_scientific_journal-2511	117	42	purification	purification	NOUN
american_scientific_journal-2511	117	43	to	to	PART
american_scientific_journal-2511	117	44	reduce	reduce	VERB
american_scientific_journal-2511	117	45	undesirable	undesirable	ADJ
american_scientific_journal-2511	117	46	band	band	NOUN
american_scientific_journal-2511	117	47	,	,	PUNCT
american_scientific_journal-2511	117	48	deae	deae	PROPN
american_scientific_journal-2511	117	49	sepharose	sepharose	NOUN
american_scientific_journal-2511	117	50	column	column	NOUN
american_scientific_journal-2511	117	51	was	be	AUX
american_scientific_journal-2511	117	52	used	use	VERB
american_scientific_journal-2511	117	53	after	after	ADP
american_scientific_journal-2511	117	54	treatment	treatment	NOUN
american_scientific_journal-2511	117	55	with	with	ADP
american_scientific_journal-2511	117	56	factor	factor	NOUN
american_scientific_journal-2511	117	57	xa	xa	PROPN
american_scientific_journal-2511	117	58	which	which	PRON
american_scientific_journal-2511	117	59	lead	lead	VERB
american_scientific_journal-2511	117	60	to	to	PART
american_scientific_journal-2511	117	61	remove	remove	VERB
american_scientific_journal-2511	117	62	his	his	PRON
american_scientific_journal-2511	117	63	-	-	PUNCT
american_scientific_journal-2511	117	64	tag	tag	NOUN
american_scientific_journal-2511	117	65	and	and	CCONJ
american_scientific_journal-2511	117	66	reduced	reduce	VERB
american_scientific_journal-2511	117	67	protein	protein	NOUN
american_scientific_journal-2511	117	68	molecular	molecular	ADJ
american_scientific_journal-2511	117	69	weight	weight	NOUN
american_scientific_journal-2511	117	70	to	to	ADP
american_scientific_journal-2511	117	71	~9.2	~9.2	PROPN
american_scientific_journal-2511	117	72	kda	kda	NOUN
american_scientific_journal-2511	117	73	.	.	PUNCT
american_scientific_journal-2511	118	1	samples	sample	NOUN
american_scientific_journal-2511	118	2	collected	collect	VERB
american_scientific_journal-2511	118	3	from	from	ADP
american_scientific_journal-2511	118	4	deae	deae	NOUN
american_scientific_journal-2511	118	5	sepharose	sepharose	NOUN
american_scientific_journal-2511	118	6	ion	ion	NOUN
american_scientific_journal-2511	118	7	exchange	exchange	NOUN
american_scientific_journal-2511	118	8	chromatography	chromatography	NOUN
american_scientific_journal-2511	118	9	detected	detect	VERB
american_scientific_journal-2511	118	10	the	the	DET
american_scientific_journal-2511	118	11	isolation	isolation	NOUN
american_scientific_journal-2511	118	12	of	of	ADP
american_scientific_journal-2511	118	13	protein	protein	NOUN
american_scientific_journal-2511	118	14	patterns	pattern	NOUN
american_scientific_journal-2511	118	15	,	,	PUNCT
american_scientific_journal-2511	118	16	by	by	ADP
american_scientific_journal-2511	118	17	the	the	DET
american_scientific_journal-2511	118	18	analysis	analysis	NOUN
american_scientific_journal-2511	118	19	of	of	ADP
american_scientific_journal-2511	118	20	the	the	DET
american_scientific_journal-2511	118	21	result	result	NOUN
american_scientific_journal-2511	118	22	with	with	ADP
american_scientific_journal-2511	118	23	15	15	NUM
american_scientific_journal-2511	118	24	%	%	NOUN
american_scientific_journal-2511	118	25	sds	sds	NOUN
american_scientific_journal-2511	118	26	-	-	PUNCT
american_scientific_journal-2511	118	27	page	page	NOUN
american_scientific_journal-2511	118	28	,	,	PUNCT
american_scientific_journal-2511	118	29	hirudin	hirudin	VERB
american_scientific_journal-2511	118	30	give	give	VERB
american_scientific_journal-2511	118	31	a	a	DET
american_scientific_journal-2511	118	32	sharp	sharp	ADJ
american_scientific_journal-2511	118	33	band	band	NOUN
american_scientific_journal-2511	118	34	on	on	ADP
american_scientific_journal-2511	118	35	concentration	concentration	NOUN
american_scientific_journal-2511	118	36	of	of	ADP
american_scientific_journal-2511	118	37	nacl	nacl	NOUN
american_scientific_journal-2511	118	38	0.2	0.2	NUM
american_scientific_journal-2511	118	39	-	-	SYM
american_scientific_journal-2511	118	40	0.4	0.4	NUM
american_scientific_journal-2511	118	41	m	m	NOUN
american_scientific_journal-2511	118	42	.	.	PUNCT
american_scientific_journal-2511	119	1	3.10	3.10	NUM
american_scientific_journal-2511	119	2	purification	purification	NOUN
american_scientific_journal-2511	119	3	by	by	ADP
american_scientific_journal-2511	119	4	sp	sp	ADP
american_scientific_journal-2511	119	5	sepharose	sepharose	NOUN
american_scientific_journal-2511	119	6	column	column	NOUN
american_scientific_journal-2511	119	7	the	the	DET
american_scientific_journal-2511	119	8	analysis	analysis	NOUN
american_scientific_journal-2511	119	9	on	on	ADP
american_scientific_journal-2511	119	10	15	15	NUM
american_scientific_journal-2511	119	11	%	%	NOUN
american_scientific_journal-2511	119	12	sds	sds	NOUN
american_scientific_journal-2511	119	13	-	-	PUNCT
american_scientific_journal-2511	119	14	page	page	NOUN
american_scientific_journal-2511	119	15	for	for	ADP
american_scientific_journal-2511	119	16	the	the	DET
american_scientific_journal-2511	119	17	sp	sp	NOUN
american_scientific_journal-2511	119	18	sepharose	sepharose	NOUN
american_scientific_journal-2511	119	19	column	column	NOUN
american_scientific_journal-2511	119	20	results	result	NOUN
american_scientific_journal-2511	119	21	shown	show	VERB
american_scientific_journal-2511	119	22	the	the	DET
american_scientific_journal-2511	119	23	presence	presence	NOUN
american_scientific_journal-2511	119	24	of	of	ADP
american_scientific_journal-2511	119	25	hirudin	hirudin	VERB
american_scientific_journal-2511	119	26	protein	protein	NOUN
american_scientific_journal-2511	119	27	in	in	ADP
american_scientific_journal-2511	119	28	elution	elution	NOUN
american_scientific_journal-2511	119	29	buffer	buffer	NOUN
american_scientific_journal-2511	119	30	ph=8	ph=8	NOUN
american_scientific_journal-2511	119	31	,	,	PUNCT
american_scientific_journal-2511	119	32	figure	figure	NOUN
american_scientific_journal-2511	119	33	12	12	NUM
american_scientific_journal-2511	119	34	.	.	PUNCT
american_scientific_journal-2511	120	1	figure	figure	NOUN
american_scientific_journal-2511	120	2	12	12	NUM
american_scientific_journal-2511	120	3	:	:	PUNCT
american_scientific_journal-2511	120	4	coomassie	coomassie	PROPN
american_scientific_journal-2511	120	5	blue	blue	PROPN
american_scientific_journal-2511	120	6	stained	stain	VERB
american_scientific_journal-2511	120	7	sds	sds	PROPN
american_scientific_journal-2511	120	8	/	/	SYM
american_scientific_journal-2511	120	9	polyacrylamide	polyacrylamide	NOUN
american_scientific_journal-2511	120	10	gel	gel	NOUN
american_scientific_journal-2511	120	11	profile	profile	NOUN
american_scientific_journal-2511	120	12	of	of	ADP
american_scientific_journal-2511	120	13	protein	protein	NOUN
american_scientific_journal-2511	120	14	from	from	ADP
american_scientific_journal-2511	120	15	sp	sp	ADP
american_scientific_journal-2511	120	16	sepharose	sepharose	NOUN
american_scientific_journal-2511	120	17	column	column	NOUN
american_scientific_journal-2511	120	18	for	for	ADP
american_scientific_journal-2511	120	19	hirudin	hirudin	VERB
american_scientific_journal-2511	120	20	protein	protein	NOUN
american_scientific_journal-2511	120	21	m	m	NOUN
american_scientific_journal-2511	120	22	:	:	PUNCT
american_scientific_journal-2511	120	23	marker	marker	NOUN
american_scientific_journal-2511	120	24	protein	protein	NOUN
american_scientific_journal-2511	120	25	10kda	10kda	NOUN
american_scientific_journal-2511	120	26	.	.	PUNCT
american_scientific_journal-2511	121	1	lane1	lane1	PROPN
american_scientific_journal-2511	121	2	:	:	PUNCT
american_scientific_journal-2511	122	1	ph=4.5,lane2	ph=4.5,lane2	PRON
american_scientific_journal-2511	122	2	:	:	PUNCT
american_scientific_journal-2511	122	3	ph=7,lane3	ph=7,lane3	ADJ
american_scientific_journal-2511	122	4	:	:	PUNCT
american_scientific_journal-2511	122	5	ph=8	ph=8	NOUN
american_scientific_journal-2511	122	6	hirudin	hirudin	VERB
american_scientific_journal-2511	122	7	band	band	NOUN
american_scientific_journal-2511	122	8	~9.2	~9.2	X
american_scientific_journal-2511	122	9	appear	appear	VERB
american_scientific_journal-2511	122	10	]	]	PUNCT
american_scientific_journal-2511	122	11	.	.	PUNCT
american_scientific_journal-2511	123	1	american	american	PROPN
american_scientific_journal-2511	123	2	scientific	scientific	ADJ
american_scientific_journal-2511	123	3	research	research	PROPN
american_scientific_journal-2511	123	4	journal	journal	NOUN
american_scientific_journal-2511	123	5	for	for	ADP
american_scientific_journal-2511	123	6	engineering	engineering	NOUN
american_scientific_journal-2511	123	7	,	,	PUNCT
american_scientific_journal-2511	123	8	technology	technology	NOUN
american_scientific_journal-2511	123	9	,	,	PUNCT
american_scientific_journal-2511	123	10	and	and	CCONJ
american_scientific_journal-2511	123	11	sciences	science	NOUN
american_scientific_journal-2511	123	12	(	(	PUNCT
american_scientific_journal-2511	123	13	asrjets	asrjet	NOUN
american_scientific_journal-2511	123	14	)	)	PUNCT
american_scientific_journal-2511	123	15	(	(	PUNCT
american_scientific_journal-2511	123	16	2017	2017	NUM
american_scientific_journal-2511	123	17	)	)	PUNCT
american_scientific_journal-2511	123	18	volume	volume	NOUN
american_scientific_journal-2511	123	19	27	27	NUM
american_scientific_journal-2511	123	20	,	,	PUNCT
american_scientific_journal-2511	123	21	no	no	DET
american_scientific_journal-2511	123	22	1	1	NUM
american_scientific_journal-2511	123	23	,	,	PUNCT
american_scientific_journal-2511	123	24	pp	pp	ADV
american_scientific_journal-2511	123	25	25	25	NUM
american_scientific_journal-2511	123	26	-	-	SYM
american_scientific_journal-2511	123	27	42	42	NUM
american_scientific_journal-2511	123	28	36	36	NUM
american_scientific_journal-2511	123	29	3.11	3.11	NUM
american_scientific_journal-2511	123	30	elisa	elisa	NOUN
american_scientific_journal-2511	123	31	immunoassay	immunoassay	NOUN
american_scientific_journal-2511	123	32	for	for	ADP
american_scientific_journal-2511	123	33	hirudin	hirudin	VERB
american_scientific_journal-2511	123	34	the	the	DET
american_scientific_journal-2511	123	35	amount	amount	NOUN
american_scientific_journal-2511	123	36	of	of	ADP
american_scientific_journal-2511	123	37	hirudin	hirudin	NOUN
american_scientific_journal-2511	123	38	in	in	ADP
american_scientific_journal-2511	123	39	the	the	DET
american_scientific_journal-2511	123	40	solution	solution	NOUN
american_scientific_journal-2511	123	41	was	be	AUX
american_scientific_journal-2511	123	42	easy	easy	ADV
american_scientific_journal-2511	123	43	assayed	assayed	ADJ
american_scientific_journal-2511	123	44	by	by	ADP
american_scientific_journal-2511	123	45	elisa	elisa	NOUN
american_scientific_journal-2511	123	46	,	,	PUNCT
american_scientific_journal-2511	123	47	by	by	ADP
american_scientific_journal-2511	123	48	drawing	draw	VERB
american_scientific_journal-2511	123	49	standard	standard	ADJ
american_scientific_journal-2511	123	50	curve	curve	NOUN
american_scientific_journal-2511	123	51	,	,	PUNCT
american_scientific_journal-2511	123	52	the	the	DET
american_scientific_journal-2511	123	53	concentration	concentration	NOUN
american_scientific_journal-2511	123	54	value	value	NOUN
american_scientific_journal-2511	123	55	as	as	ADP
american_scientific_journal-2511	123	56	abscissa	abscissa	ADJ
american_scientific_journal-2511	123	57	and	and	CCONJ
american_scientific_journal-2511	123	58	od	od	NUM
american_scientific_journal-2511	123	59	value	value	NOUN
american_scientific_journal-2511	123	60	as	as	ADP
american_scientific_journal-2511	123	61	vertical	vertical	ADJ
american_scientific_journal-2511	123	62	coordinate	coordinate	NOUN
american_scientific_journal-2511	123	63	,	,	PUNCT
american_scientific_journal-2511	123	64	the	the	DET
american_scientific_journal-2511	123	65	concentration	concentration	NOUN
american_scientific_journal-2511	123	66	determined	determine	VERB
american_scientific_journal-2511	123	67	which	which	PRON
american_scientific_journal-2511	123	68	reached	reach	VERB
american_scientific_journal-2511	123	69	1.35ng	1.35ng	NUM
american_scientific_journal-2511	123	70	as	as	SCONJ
american_scientific_journal-2511	123	71	showed	show	VERB
american_scientific_journal-2511	123	72	in	in	ADP
american_scientific_journal-2511	123	73	figure	figure	NOUN
american_scientific_journal-2511	123	74	13	13	NUM
american_scientific_journal-2511	123	75	.	.	PUNCT
american_scientific_journal-2511	124	1	figure	figure	VERB
american_scientific_journal-2511	124	2	13	13	NUM
american_scientific_journal-2511	124	3	:	:	PUNCT
american_scientific_journal-2511	124	4	the	the	DET
american_scientific_journal-2511	124	5	standard	standard	ADJ
american_scientific_journal-2511	124	6	curve	curve	NOUN
american_scientific_journal-2511	124	7	with	with	ADP
american_scientific_journal-2511	124	8	hirudin	hirudin	VERB
american_scientific_journal-2511	124	9	elisa	elisa	NOUN
american_scientific_journal-2511	124	10	■	■	X
american_scientific_journal-2511	124	11	1.35ng	1.35ng	NUM
american_scientific_journal-2511	124	12	hirudin	hirudin	VERB
american_scientific_journal-2511	124	13	3.12	3.12	NUM
american_scientific_journal-2511	124	14	detection	detection	NOUN
american_scientific_journal-2511	124	15	of	of	ADP
american_scientific_journal-2511	124	16	hirudin	hirudin	VERB
american_scientific_journal-2511	124	17	activity	activity	NOUN
american_scientific_journal-2511	124	18	by	by	ADP
american_scientific_journal-2511	124	19	titration	titration	NOUN
american_scientific_journal-2511	124	20	with	with	ADP
american_scientific_journal-2511	124	21	thrombin	thrombin	NOUN
american_scientific_journal-2511	124	22	table	table	NOUN
american_scientific_journal-2511	124	23	1	1	NUM
american_scientific_journal-2511	124	24	illustrate	illustrate	VERB
american_scientific_journal-2511	124	25	the	the	DET
american_scientific_journal-2511	124	26	result	result	NOUN
american_scientific_journal-2511	124	27	for	for	ADP
american_scientific_journal-2511	124	28	hirudin	hirudin	VERB
american_scientific_journal-2511	124	29	activity	activity	NOUN
american_scientific_journal-2511	124	30	according	accord	VERB
american_scientific_journal-2511	124	31	to	to	ADP
american_scientific_journal-2511	124	32	thrombin	thrombin	PROPN
american_scientific_journal-2511	124	33	titration	titration	NOUN
american_scientific_journal-2511	124	34	method	method	NOUN
american_scientific_journal-2511	124	35	table	table	NOUN
american_scientific_journal-2511	124	36	1	1	NUM
american_scientific_journal-2511	124	37	:	:	PUNCT
american_scientific_journal-2511	124	38	thrombin	thrombin	NOUN
american_scientific_journal-2511	124	39	volume	volume	NOUN
american_scientific_journal-2511	124	40	for	for	ADP
american_scientific_journal-2511	124	41	clot	clot	NOUN
american_scientific_journal-2511	124	42	formation	formation	NOUN
american_scientific_journal-2511	124	43	in	in	ADP
american_scientific_journal-2511	124	44	thrombin	thrombin	NOUN
american_scientific_journal-2511	124	45	titration	titration	NOUN
american_scientific_journal-2511	124	46	with	with	ADP
american_scientific_journal-2511	124	47	hirudin	hirudin	VERB
american_scientific_journal-2511	124	48	100µl	100µl	ADJ
american_scientific_journal-2511	124	49	hirudin	hirudin	NOUN
american_scientific_journal-2511	124	50	r+200µl	r+200µl	NOUN
american_scientific_journal-2511	124	51	fibrinogen	fibrinogen	NOUN
american_scientific_journal-2511	124	52	control1/	control1/	ADJ
american_scientific_journal-2511	124	53	200µl	200µl	ADJ
american_scientific_journal-2511	124	54	fibrinogen+100µl	fibrinogen+100µl	NOUN
american_scientific_journal-2511	124	55	water	water	NOUN
american_scientific_journal-2511	124	56	control2/	control2/	NOUN
american_scientific_journal-2511	124	57	200µl	200µl	ADJ
american_scientific_journal-2511	124	58	fibrinogen	fibrinogen	NOUN
american_scientific_journal-2511	124	59	t	t	NOUN
american_scientific_journal-2511	124	60	hrom	hrom	PROPN
american_scientific_journal-2511	124	61	bin	bin	PROPN
american_scientific_journal-2511	124	62	titration	titration	PROPN
american_scientific_journal-2511	124	63	volum	volum	NOUN
american_scientific_journal-2511	124	64	e/µl	e/µl	PROPN
american_scientific_journal-2511	124	65	360	360	NUM
american_scientific_journal-2511	124	66	µl	µl	ADP
american_scientific_journal-2511	124	67	5	5	NUM
american_scientific_journal-2511	124	68	µl	µl	ADP
american_scientific_journal-2511	124	69	5	5	NUM
american_scientific_journal-2511	124	70	µl	µl	ADP
american_scientific_journal-2511	124	71	4	4	NUM
american_scientific_journal-2511	124	72	.	.	PUNCT
american_scientific_journal-2511	125	1	discussion	discussion	NOUN
american_scientific_journal-2511	125	2	the	the	DET
american_scientific_journal-2511	125	3	medicinal	medicinal	ADJ
american_scientific_journal-2511	125	4	leech	leech	NOUN
american_scientific_journal-2511	125	5	is	be	AUX
american_scientific_journal-2511	125	6	the	the	DET
american_scientific_journal-2511	125	7	most	most	ADV
american_scientific_journal-2511	125	8	famous	famous	ADJ
american_scientific_journal-2511	125	9	representative	representative	NOUN
american_scientific_journal-2511	125	10	of	of	ADP
american_scientific_journal-2511	125	11	the	the	DET
american_scientific_journal-2511	125	12	hirudinea	hirudinea	NOUN
american_scientific_journal-2511	125	13	,	,	PUNCT
american_scientific_journal-2511	125	14	it	it	PRON
american_scientific_journal-2511	125	15	is	be	AUX
american_scientific_journal-2511	125	16	one	one	NUM
american_scientific_journal-2511	125	17	of	of	ADP
american_scientific_journal-2511	125	18	few	few	ADJ
american_scientific_journal-2511	125	19	invertebrates	invertebrate	NOUN
american_scientific_journal-2511	125	20	widely	widely	ADV
american_scientific_journal-2511	125	21	used	use	VERB
american_scientific_journal-2511	125	22	in	in	ADP
american_scientific_journal-2511	125	23	medicine	medicine	NOUN
american_scientific_journal-2511	125	24	and	and	CCONJ
american_scientific_journal-2511	125	25	as	as	ADP
american_scientific_journal-2511	125	26	a	a	DET
american_scientific_journal-2511	125	27	scientific	scientific	ADJ
american_scientific_journal-2511	125	28	model	model	NOUN
american_scientific_journal-2511	125	29	object	object	NOUN
american_scientific_journal-2511	125	30	.	.	PUNCT
american_scientific_journal-2511	126	1	the	the	DET
american_scientific_journal-2511	126	2	medicinal	medicinal	ADJ
american_scientific_journal-2511	126	3	leech	leech	NOUN
american_scientific_journal-2511	126	4	hirudo	hirudo	NOUN
american_scientific_journal-2511	126	5	medicinalis	medicinali	NOUN
american_scientific_journal-2511	126	6	is	be	AUX
american_scientific_journal-2511	126	7	the	the	DET
american_scientific_journal-2511	126	8	most	most	ADV
american_scientific_journal-2511	126	9	prominent	prominent	ADJ
american_scientific_journal-2511	126	10	representative	representative	NOUN
american_scientific_journal-2511	126	11	of	of	ADP
american_scientific_journal-2511	126	12	the	the	DET
american_scientific_journal-2511	126	13	class	class	NOUN
american_scientific_journal-2511	126	14	hirudinea	hirudinea	NOUN
american_scientific_journal-2511	126	15	and	and	CCONJ
american_scientific_journal-2511	126	16	one	one	NUM
american_scientific_journal-2511	126	17	of	of	ADP
american_scientific_journal-2511	126	18	the	the	DET
american_scientific_journal-2511	126	19	most	most	ADV
american_scientific_journal-2511	126	20	renowned	renowned	ADJ
american_scientific_journal-2511	126	21	invertebrates	invertebrate	NOUN
american_scientific_journal-2511	126	22	infamous	infamous	ADJ
american_scientific_journal-2511	126	23	for	for	ADP
american_scientific_journal-2511	126	24	its	its	PRON
american_scientific_journal-2511	126	25	ectoparasitic	ectoparasitic	ADJ
american_scientific_journal-2511	126	26	bloodsucking	bloodsucking	NOUN
american_scientific_journal-2511	126	27	,	,	PUNCT
american_scientific_journal-2511	126	28	but	but	CCONJ
american_scientific_journal-2511	126	29	it	it	PRON
american_scientific_journal-2511	126	30	's	be	AUX
american_scientific_journal-2511	126	31	not	not	PART
american_scientific_journal-2511	126	32	the	the	DET
american_scientific_journal-2511	126	33	only	only	ADJ
american_scientific_journal-2511	126	34	species	specie	NOUN
american_scientific_journal-2511	126	35	,	,	PUNCT
american_scientific_journal-2511	126	36	this	this	DET
american_scientific_journal-2511	126	37	class	class	NOUN
american_scientific_journal-2511	126	38	contain	contain	VERB
american_scientific_journal-2511	126	39	many	many	ADJ
american_scientific_journal-2511	126	40	medical	medical	ADJ
american_scientific_journal-2511	126	41	species	specie	NOUN
american_scientific_journal-2511	126	42	spread	spread	VERB
american_scientific_journal-2511	126	43	in	in	ADP
american_scientific_journal-2511	126	44	1.35	1.35	NUM
american_scientific_journal-2511	126	45	0	0	NUM
american_scientific_journal-2511	126	46	0.5	0.5	NUM
american_scientific_journal-2511	126	47	1	1	NUM
american_scientific_journal-2511	126	48	1.5	1.5	NUM
american_scientific_journal-2511	126	49	2	2	NUM
american_scientific_journal-2511	126	50	2.5	2.5	NUM
american_scientific_journal-2511	126	51	3	3	NUM
american_scientific_journal-2511	126	52	3.5	3.5	NUM
american_scientific_journal-2511	126	53	4	4	NUM
american_scientific_journal-2511	126	54	0	0	NUM
american_scientific_journal-2511	126	55	5	5	NUM
american_scientific_journal-2511	126	56	10	10	NUM
american_scientific_journal-2511	126	57	15	15	NUM
american_scientific_journal-2511	126	58	20	20	NUM
american_scientific_journal-2511	126	59	25	25	NUM
american_scientific_journal-2511	126	60	ab	ab	PROPN
american_scientific_journal-2511	126	61	s.	s.	PROPN
american_scientific_journal-2511	126	62	45	45	NUM
american_scientific_journal-2511	126	63	0	0	NUM
american_scientific_journal-2511	126	64	nm	nm	NOUN
american_scientific_journal-2511	126	65	.	.	PUNCT
american_scientific_journal-2511	127	1	con.stand.hirudin	con.stand.hirudin	VERB
american_scientific_journal-2511	127	2	ng	ng	PROPN
american_scientific_journal-2511	127	3	/	/	SYM
american_scientific_journal-2511	127	4	ml	ml	PROPN
american_scientific_journal-2511	127	5	american	american	ADJ
american_scientific_journal-2511	127	6	scientific	scientific	ADJ
american_scientific_journal-2511	127	7	research	research	NOUN
american_scientific_journal-2511	127	8	journal	journal	NOUN
american_scientific_journal-2511	127	9	for	for	ADP
american_scientific_journal-2511	127	10	engineering	engineering	NOUN
american_scientific_journal-2511	127	11	,	,	PUNCT
american_scientific_journal-2511	127	12	technology	technology	NOUN
american_scientific_journal-2511	127	13	,	,	PUNCT
american_scientific_journal-2511	127	14	and	and	CCONJ
american_scientific_journal-2511	127	15	sciences	science	NOUN
american_scientific_journal-2511	127	16	(	(	PUNCT
american_scientific_journal-2511	127	17	asrjets	asrjet	NOUN
american_scientific_journal-2511	127	18	)	)	PUNCT
american_scientific_journal-2511	127	19	(	(	PUNCT
american_scientific_journal-2511	127	20	2017	2017	NUM
american_scientific_journal-2511	127	21	)	)	PUNCT
american_scientific_journal-2511	127	22	volume	volume	NOUN
american_scientific_journal-2511	127	23	27	27	NUM
american_scientific_journal-2511	127	24	,	,	PUNCT
american_scientific_journal-2511	127	25	no	no	DET
american_scientific_journal-2511	127	26	1	1	NUM
american_scientific_journal-2511	127	27	,	,	PUNCT
american_scientific_journal-2511	127	28	pp	pp	ADV
american_scientific_journal-2511	127	29	25	25	NUM
american_scientific_journal-2511	127	30	-	-	SYM
american_scientific_journal-2511	127	31	42	42	NUM
american_scientific_journal-2511	127	32	37	37	NUM
american_scientific_journal-2511	127	33	transcaucasia	transcaucasia	NOUN
american_scientific_journal-2511	127	34	and	and	CCONJ
american_scientific_journal-2511	127	35	iran	iran	PROPN
american_scientific_journal-2511	127	36	as	as	ADP
american_scientific_journal-2511	127	37	h.	h.	NOUN
american_scientific_journal-2511	127	38	orientalis	orientalis	PROPN
american_scientific_journal-2511	127	39	and	and	CCONJ
american_scientific_journal-2511	127	40	in	in	ADP
american_scientific_journal-2511	127	41	south	south	PROPN
american_scientific_journal-2511	127	42	eastern	eastern	PROPN
american_scientific_journal-2511	127	43	europe	europe	PROPN
american_scientific_journal-2511	127	44	and	and	CCONJ
american_scientific_journal-2511	127	45	turkey	turkey	PROPN
american_scientific_journal-2511	127	46	as	as	ADP
american_scientific_journal-2511	127	47	h.	h.	PROPN
american_scientific_journal-2511	127	48	verbana	verbana	PROPN
american_scientific_journal-2511	127	49	.	.	PUNCT
american_scientific_journal-2511	128	1	despite	despite	SCONJ
american_scientific_journal-2511	128	2	all	all	DET
american_scientific_journal-2511	128	3	attention	attention	NOUN
american_scientific_journal-2511	128	4	,	,	PUNCT
american_scientific_journal-2511	128	5	there	there	PRON
american_scientific_journal-2511	128	6	is	be	VERB
american_scientific_journal-2511	128	7	confusion	confusion	NOUN
american_scientific_journal-2511	128	8	regarding	regard	VERB
american_scientific_journal-2511	128	9	the	the	DET
american_scientific_journal-2511	128	10	taxonomic	taxonomic	ADJ
american_scientific_journal-2511	128	11	status	status	NOUN
american_scientific_journal-2511	128	12	of	of	ADP
american_scientific_journal-2511	128	13	different	different	ADJ
american_scientific_journal-2511	128	14	morphological	morphological	ADJ
american_scientific_journal-2511	128	15	forms	form	NOUN
american_scientific_journal-2511	128	16	[	[	X
american_scientific_journal-2511	128	17	13	13	NUM
american_scientific_journal-2511	128	18	]	]	PUNCT
american_scientific_journal-2511	128	19	.	.	PUNCT
american_scientific_journal-2511	129	1	the	the	DET
american_scientific_journal-2511	129	2	small	small	ADJ
american_scientific_journal-2511	129	3	subunit	subunit	NOUN
american_scientific_journal-2511	129	4	(	(	PUNCT
american_scientific_journal-2511	129	5	18s	18s	NUM
american_scientific_journal-2511	129	6	)	)	PUNCT
american_scientific_journal-2511	129	7	ribosomal	ribosomal	NOUN
american_scientific_journal-2511	129	8	gene	gene	NOUN
american_scientific_journal-2511	129	9	is	be	AUX
american_scientific_journal-2511	129	10	one	one	NUM
american_scientific_journal-2511	129	11	of	of	ADP
american_scientific_journal-2511	129	12	the	the	DET
american_scientific_journal-2511	129	13	most	most	ADV
american_scientific_journal-2511	129	14	frequently	frequently	ADV
american_scientific_journal-2511	129	15	used	use	VERB
american_scientific_journal-2511	129	16	genes	gene	NOUN
american_scientific_journal-2511	129	17	in	in	ADP
american_scientific_journal-2511	129	18	phylogenetic	phylogenetic	ADJ
american_scientific_journal-2511	129	19	studies	study	NOUN
american_scientific_journal-2511	129	20	and	and	CCONJ
american_scientific_journal-2511	129	21	an	an	DET
american_scientific_journal-2511	129	22	important	important	ADJ
american_scientific_journal-2511	129	23	marker	marker	NOUN
american_scientific_journal-2511	129	24	for	for	ADP
american_scientific_journal-2511	129	25	random	random	ADJ
american_scientific_journal-2511	129	26	target	target	NOUN
american_scientific_journal-2511	129	27	pcr	pcr	NOUN
american_scientific_journal-2511	129	28	in	in	ADP
american_scientific_journal-2511	129	29	environmental	environmental	ADJ
american_scientific_journal-2511	129	30	biodiversity	biodiversity	NOUN
american_scientific_journal-2511	129	31	screening	screening	NOUN
american_scientific_journal-2511	129	32	,	,	PUNCT
american_scientific_journal-2511	129	33	in	in	ADP
american_scientific_journal-2511	129	34	general	general	ADJ
american_scientific_journal-2511	129	35	this	this	DET
american_scientific_journal-2511	129	36	gene	gene	NOUN
american_scientific_journal-2511	129	37	sequences	sequence	NOUN
american_scientific_journal-2511	129	38	are	be	AUX
american_scientific_journal-2511	129	39	easy	easy	ADJ
american_scientific_journal-2511	129	40	to	to	PART
american_scientific_journal-2511	129	41	access	access	VERB
american_scientific_journal-2511	129	42	due	due	ADJ
american_scientific_journal-2511	129	43	to	to	ADP
american_scientific_journal-2511	129	44	highly	highly	ADV
american_scientific_journal-2511	129	45	conserved	conserved	ADJ
american_scientific_journal-2511	129	46	flanking	flank	VERB
american_scientific_journal-2511	129	47	regions	region	NOUN
american_scientific_journal-2511	129	48	allowing	allow	VERB
american_scientific_journal-2511	129	49	for	for	ADP
american_scientific_journal-2511	129	50	the	the	DET
american_scientific_journal-2511	129	51	use	use	NOUN
american_scientific_journal-2511	129	52	universal	universal	ADJ
american_scientific_journal-2511	129	53	primers	primer	NOUN
american_scientific_journal-2511	129	54	,	,	PUNCT
american_scientific_journal-2511	129	55	their	their	PRON
american_scientific_journal-2511	129	56	repetitive	repetitive	ADJ
american_scientific_journal-2511	129	57	arrangement	arrangement	NOUN
american_scientific_journal-2511	129	58	within	within	ADP
american_scientific_journal-2511	129	59	the	the	DET
american_scientific_journal-2511	129	60	genome	genome	NOUN
american_scientific_journal-2511	129	61	provides	provide	VERB
american_scientific_journal-2511	129	62	excessive	excessive	ADJ
american_scientific_journal-2511	129	63	amounts	amount	NOUN
american_scientific_journal-2511	129	64	of	of	ADP
american_scientific_journal-2511	129	65	template	template	NOUN
american_scientific_journal-2511	129	66	dna	dna	NOUN
american_scientific_journal-2511	129	67	for	for	ADP
american_scientific_journal-2511	129	68	pcr	pcr	NOUN
american_scientific_journal-2511	129	69	,	,	PUNCT
american_scientific_journal-2511	129	70	even	even	ADV
american_scientific_journal-2511	129	71	in	in	ADP
american_scientific_journal-2511	129	72	smallest	small	ADJ
american_scientific_journal-2511	129	73	organisms	organism	NOUN
american_scientific_journal-2511	129	74	.	.	PUNCT
american_scientific_journal-2511	130	1	the	the	DET
american_scientific_journal-2511	130	2	18s	18s	NUM
american_scientific_journal-2511	130	3	rrna	rrna	PROPN
american_scientific_journal-2511	130	4	gene	gene	NOUN
american_scientific_journal-2511	130	5	is	be	AUX
american_scientific_journal-2511	130	6	part	part	NOUN
american_scientific_journal-2511	130	7	of	of	ADP
american_scientific_journal-2511	130	8	the	the	DET
american_scientific_journal-2511	130	9	ribosomal	ribosomal	ADJ
american_scientific_journal-2511	130	10	functional	functional	ADJ
american_scientific_journal-2511	130	11	core	core	NOUN
american_scientific_journal-2511	130	12	and	and	CCONJ
american_scientific_journal-2511	130	13	is	be	AUX
american_scientific_journal-2511	130	14	exposed	expose	VERB
american_scientific_journal-2511	130	15	to	to	ADP
american_scientific_journal-2511	130	16	similar	similar	ADJ
american_scientific_journal-2511	130	17	selective	selective	ADJ
american_scientific_journal-2511	130	18	forces	force	NOUN
american_scientific_journal-2511	130	19	in	in	ADP
american_scientific_journal-2511	130	20	all	all	DET
american_scientific_journal-2511	130	21	living	live	VERB
american_scientific_journal-2511	130	22	beings	being	NOUN
american_scientific_journal-2511	130	23	,	,	PUNCT
american_scientific_journal-2511	130	24	for	for	ADP
american_scientific_journal-2511	130	25	that	that	DET
american_scientific_journal-2511	130	26	reasons	reason	NOUN
american_scientific_journal-2511	130	27	biodiversity	biodiversity	NOUN
american_scientific_journal-2511	130	28	studies	study	NOUN
american_scientific_journal-2511	130	29	are	be	AUX
american_scientific_journal-2511	130	30	commonly	commonly	ADV
american_scientific_journal-2511	130	31	conducted	conduct	VERB
american_scientific_journal-2511	130	32	using	use	VERB
american_scientific_journal-2511	130	33	18s	18	NOUN
american_scientific_journal-2511	130	34	rrna	rrna	PROPN
american_scientific_journal-2511	130	35	genes	gene	NOUN
american_scientific_journal-2511	130	36	[	[	X
american_scientific_journal-2511	130	37	14,15	14,15	NUM
american_scientific_journal-2511	130	38	]	]	PUNCT
american_scientific_journal-2511	130	39	.	.	PUNCT
american_scientific_journal-2511	131	1	moreover	moreover	ADV
american_scientific_journal-2511	131	2	the	the	DET
american_scientific_journal-2511	131	3	mitochondrial	mitochondrial	ADJ
american_scientific_journal-2511	131	4	gene	gene	NOUN
american_scientific_journal-2511	131	5	,	,	PUNCT
american_scientific_journal-2511	131	6	cytochrome	cytochrome	NOUN
american_scientific_journal-2511	131	7	c	c	NOUN
american_scientific_journal-2511	131	8	oxidase	oxidase	NOUN
american_scientific_journal-2511	131	9	subunit	subunit	NOUN
american_scientific_journal-2511	131	10	1	1	NUM
american_scientific_journal-2511	131	11	(	(	PUNCT
american_scientific_journal-2511	131	12	coi	coi	NOUN
american_scientific_journal-2511	131	13	)	)	PUNCT
american_scientific_journal-2511	131	14	,	,	PUNCT
american_scientific_journal-2511	131	15	is	be	AUX
american_scientific_journal-2511	131	16	also	also	ADV
american_scientific_journal-2511	131	17	important	important	ADJ
american_scientific_journal-2511	131	18	gene	gene	NOUN
american_scientific_journal-2511	131	19	used	use	VERB
american_scientific_journal-2511	131	20	in	in	ADP
american_scientific_journal-2511	131	21	animal	animal	NOUN
american_scientific_journal-2511	131	22	classification	classification	NOUN
american_scientific_journal-2511	131	23	,	,	PUNCT
american_scientific_journal-2511	131	24	while	while	SCONJ
american_scientific_journal-2511	131	25	past	past	ADJ
american_scientific_journal-2511	131	26	work	work	NOUN
american_scientific_journal-2511	131	27	has	have	AUX
american_scientific_journal-2511	131	28	validated	validate	VERB
american_scientific_journal-2511	131	29	the	the	DET
american_scientific_journal-2511	131	30	ability	ability	NOUN
american_scientific_journal-2511	131	31	of	of	ADP
american_scientific_journal-2511	131	32	coi	coi	NOUN
american_scientific_journal-2511	131	33	sequences	sequence	NOUN
american_scientific_journal-2511	131	34	to	to	PART
american_scientific_journal-2511	131	35	diagnose	diagnose	VERB
american_scientific_journal-2511	131	36	species	specie	NOUN
american_scientific_journal-2511	131	37	in	in	ADP
american_scientific_journal-2511	131	38	certain	certain	ADJ
american_scientific_journal-2511	131	39	taxonomic	taxonomic	ADJ
american_scientific_journal-2511	131	40	groups	group	NOUN
american_scientific_journal-2511	131	41	,	,	PUNCT
american_scientific_journal-2511	131	42	the	the	DET
american_scientific_journal-2511	131	43	present	present	ADJ
american_scientific_journal-2511	131	44	study	study	NOUN
american_scientific_journal-2511	131	45	extends	extend	VERB
american_scientific_journal-2511	131	46	these	these	DET
american_scientific_journal-2511	131	47	analyses	analysis	NOUN
american_scientific_journal-2511	131	48	across	across	ADP
american_scientific_journal-2511	131	49	the	the	DET
american_scientific_journal-2511	131	50	animal	animal	NOUN
american_scientific_journal-2511	131	51	kingdom	kingdom	NOUN
american_scientific_journal-2511	131	52	.	.	PUNCT
american_scientific_journal-2511	132	1	the	the	DET
american_scientific_journal-2511	132	2	result	result	NOUN
american_scientific_journal-2511	132	3	indicates	indicate	VERB
american_scientific_journal-2511	132	4	that	that	SCONJ
american_scientific_journal-2511	132	5	sequence	sequence	NOUN
american_scientific_journal-2511	132	6	divergences	divergence	NOUN
american_scientific_journal-2511	132	7	at	at	ADP
american_scientific_journal-2511	132	8	coi	coi	NOUN
american_scientific_journal-2511	132	9	regularly	regularly	ADV
american_scientific_journal-2511	132	10	enable	enable	VERB
american_scientific_journal-2511	132	11	the	the	DET
american_scientific_journal-2511	132	12	discrimination	discrimination	NOUN
american_scientific_journal-2511	132	13	of	of	ADP
american_scientific_journal-2511	132	14	closely	closely	ADV
american_scientific_journal-2511	132	15	allied	ally	VERB
american_scientific_journal-2511	132	16	species	specie	NOUN
american_scientific_journal-2511	132	17	in	in	ADP
american_scientific_journal-2511	132	18	all	all	DET
american_scientific_journal-2511	132	19	animal	animal	NOUN
american_scientific_journal-2511	132	20	phyla	phyla	NOUN
american_scientific_journal-2511	132	21	this	this	DET
american_scientific_journal-2511	132	22	success	success	NOUN
american_scientific_journal-2511	132	23	in	in	ADP
american_scientific_journal-2511	132	24	species	specie	NOUN
american_scientific_journal-2511	132	25	diagnosis	diagnosis	NOUN
american_scientific_journal-2511	132	26	reflects	reflect	VERB
american_scientific_journal-2511	132	27	both	both	CCONJ
american_scientific_journal-2511	132	28	the	the	DET
american_scientific_journal-2511	132	29	high	high	ADJ
american_scientific_journal-2511	132	30	rates	rate	NOUN
american_scientific_journal-2511	132	31	of	of	ADP
american_scientific_journal-2511	132	32	sequence	sequence	NOUN
american_scientific_journal-2511	132	33	change	change	NOUN
american_scientific_journal-2511	132	34	at	at	ADP
american_scientific_journal-2511	132	35	coi	coi	NOUN
american_scientific_journal-2511	132	36	in	in	ADP
american_scientific_journal-2511	132	37	most	most	ADJ
american_scientific_journal-2511	132	38	animal	animal	NOUN
american_scientific_journal-2511	132	39	groups	group	NOUN
american_scientific_journal-2511	132	40	.	.	PUNCT
american_scientific_journal-2511	133	1	the	the	DET
american_scientific_journal-2511	133	2	diversity	diversity	NOUN
american_scientific_journal-2511	133	3	in	in	ADP
american_scientific_journal-2511	133	4	the	the	DET
american_scientific_journal-2511	133	5	amino	amino	NOUN
american_scientific_journal-2511	133	6	acid	acid	NOUN
american_scientific_journal-2511	133	7	sequences	sequence	NOUN
american_scientific_journal-2511	133	8	coded	code	VERB
american_scientific_journal-2511	133	9	by	by	ADP
american_scientific_journal-2511	133	10	the	the	DET
american_scientific_journal-2511	133	11	5	5	NUM
american_scientific_journal-2511	133	12	´	´	NOUN
american_scientific_journal-2511	133	13	section	section	NOUN
american_scientific_journal-2511	133	14	of	of	ADP
american_scientific_journal-2511	133	15	this	this	DET
american_scientific_journal-2511	133	16	mitochondrial	mitochondrial	ADJ
american_scientific_journal-2511	133	17	gene	gene	NOUN
american_scientific_journal-2511	133	18	was	be	AUX
american_scientific_journal-2511	133	19	sufficient	sufficient	ADJ
american_scientific_journal-2511	133	20	to	to	PART
american_scientific_journal-2511	133	21	reliably	reliably	ADV
american_scientific_journal-2511	133	22	place	place	VERB
american_scientific_journal-2511	133	23	species	specie	NOUN
american_scientific_journal-2511	133	24	into	into	ADP
american_scientific_journal-2511	133	25	higher	high	ADJ
american_scientific_journal-2511	133	26	taxonomic	taxonomic	ADJ
american_scientific_journal-2511	133	27	categories	category	NOUN
american_scientific_journal-2511	133	28	(	(	PUNCT
american_scientific_journal-2511	133	29	from	from	ADP
american_scientific_journal-2511	133	30	phyla	phyla	NOUN
american_scientific_journal-2511	133	31	to	to	ADP
american_scientific_journal-2511	133	32	order	order	VERB
american_scientific_journal-2511	133	33	)	)	PUNCT
american_scientific_journal-2511	134	1	[	[	X
american_scientific_journal-2511	134	2	16	16	NUM
american_scientific_journal-2511	134	3	]	]	PUNCT
american_scientific_journal-2511	134	4	.	.	PUNCT
american_scientific_journal-2511	135	1	results	result	NOUN
american_scientific_journal-2511	135	2	from	from	ADP
american_scientific_journal-2511	135	3	18s	18	NOUN
american_scientific_journal-2511	135	4	rdna	rdna	PROPN
american_scientific_journal-2511	135	5	and	and	CCONJ
american_scientific_journal-2511	135	6	co	co	NOUN
american_scientific_journal-2511	135	7	-	-	NOUN
american_scientific_journal-2511	135	8	i	i	PRON
american_scientific_journal-2511	135	9	genes	gene	NOUN
american_scientific_journal-2511	135	10	figures	figure	VERB
american_scientific_journal-2511	135	11	1	1	NUM
american_scientific_journal-2511	135	12	and	and	CCONJ
american_scientific_journal-2511	135	13	2	2	NUM
american_scientific_journal-2511	135	14	and	and	CCONJ
american_scientific_journal-2511	135	15	sequencing	sequencing	NOUN
american_scientific_journal-2511	135	16	approved	approve	VERB
american_scientific_journal-2511	135	17	the	the	DET
american_scientific_journal-2511	135	18	type	type	NOUN
american_scientific_journal-2511	135	19	of	of	ADP
american_scientific_journal-2511	135	20	leeches	leech	NOUN
american_scientific_journal-2511	135	21	was	be	AUX
american_scientific_journal-2511	135	22	used	use	VERB
american_scientific_journal-2511	135	23	in	in	ADP
american_scientific_journal-2511	135	24	current	current	ADJ
american_scientific_journal-2511	135	25	study	study	NOUN
american_scientific_journal-2511	135	26	is	be	AUX
american_scientific_journal-2511	135	27	h.	h.	PROPN
american_scientific_journal-2511	135	28	orientalis	orientalis	PROPN
american_scientific_journal-2511	135	29	,	,	PUNCT
american_scientific_journal-2511	135	30	which	which	PRON
american_scientific_journal-2511	135	31	is	be	AUX
american_scientific_journal-2511	135	32	medical	medical	ADJ
american_scientific_journal-2511	135	33	type	type	NOUN
american_scientific_journal-2511	135	34	spread	spread	VERB
american_scientific_journal-2511	135	35	in	in	ADP
american_scientific_journal-2511	135	36	iran	iran	PROPN
american_scientific_journal-2511	135	37	and	and	CCONJ
american_scientific_journal-2511	135	38	transcaucasia	transcaucasia	NOUN
american_scientific_journal-2511	135	39	,	,	PUNCT
american_scientific_journal-2511	135	40	this	this	DET
american_scientific_journal-2511	135	41	type	type	NOUN
american_scientific_journal-2511	135	42	of	of	ADP
american_scientific_journal-2511	135	43	leeches	leech	NOUN
american_scientific_journal-2511	135	44	used	use	VERB
american_scientific_journal-2511	135	45	in	in	ADP
american_scientific_journal-2511	135	46	these	these	DET
american_scientific_journal-2511	135	47	countries	country	NOUN
american_scientific_journal-2511	135	48	to	to	PART
american_scientific_journal-2511	135	49	treat	treat	VERB
american_scientific_journal-2511	135	50	a	a	DET
american_scientific_journal-2511	135	51	dangerous	dangerous	ADJ
american_scientific_journal-2511	135	52	complication	complication	NOUN
american_scientific_journal-2511	135	53	from	from	ADP
american_scientific_journal-2511	135	54	reconstructive	reconstructive	ADJ
american_scientific_journal-2511	135	55	microvascular	microvascular	ADJ
american_scientific_journal-2511	135	56	surgery	surgery	NOUN
american_scientific_journal-2511	135	57	is	be	AUX
american_scientific_journal-2511	135	58	venous	venous	ADJ
american_scientific_journal-2511	135	59	congestion	congestion	NOUN
american_scientific_journal-2511	135	60	,	,	PUNCT
american_scientific_journal-2511	135	61	reducing	reduce	VERB
american_scientific_journal-2511	135	62	blood	blood	NOUN
american_scientific_journal-2511	135	63	flow	flow	NOUN
american_scientific_journal-2511	135	64	,	,	PUNCT
american_scientific_journal-2511	135	65	consequently	consequently	ADV
american_scientific_journal-2511	135	66	leading	lead	VERB
american_scientific_journal-2511	135	67	to	to	ADP
american_scientific_journal-2511	135	68	tissue	tissue	NOUN
american_scientific_journal-2511	135	69	necrosis	necrosis	NOUN
american_scientific_journal-2511	135	70	and	and	CCONJ
american_scientific_journal-2511	135	71	a	a	DET
american_scientific_journal-2511	135	72	localized	localize	VERB
american_scientific_journal-2511	135	73	immune	immune	ADJ
american_scientific_journal-2511	135	74	compromised	compromise	VERB
american_scientific_journal-2511	135	75	condition	condition	NOUN
american_scientific_journal-2511	135	76	.	.	PUNCT
american_scientific_journal-2511	136	1	in	in	ADP
american_scientific_journal-2511	136	2	spite	spite	NOUN
american_scientific_journal-2511	136	3	of	of	ADP
american_scientific_journal-2511	136	4	these	these	DET
american_scientific_journal-2511	136	5	country	country	NOUN
american_scientific_journal-2511	136	6	used	use	VERB
american_scientific_journal-2511	136	7	this	this	DET
american_scientific_journal-2511	136	8	type	type	NOUN
american_scientific_journal-2511	136	9	without	without	ADP
american_scientific_journal-2511	136	10	any	any	DET
american_scientific_journal-2511	136	11	interest	interest	NOUN
american_scientific_journal-2511	136	12	about	about	ADP
american_scientific_journal-2511	136	13	the	the	DET
american_scientific_journal-2511	136	14	species	specie	NOUN
american_scientific_journal-2511	136	15	but	but	CCONJ
american_scientific_journal-2511	136	16	in	in	ADP
american_scientific_journal-2511	136	17	europe	europe	PROPN
american_scientific_journal-2511	136	18	or	or	CCONJ
american_scientific_journal-2511	136	19	united	united	ADJ
american_scientific_journal-2511	136	20	kingdom	kingdom	PROPN
american_scientific_journal-2511	136	21	specially	specially	ADV
american_scientific_journal-2511	136	22	in	in	ADP
american_scientific_journal-2511	136	23	pharmaceutical	pharmaceutical	ADJ
american_scientific_journal-2511	136	24	company	company	NOUN
american_scientific_journal-2511	136	25	which	which	PRON
american_scientific_journal-2511	136	26	insist	insist	VERB
american_scientific_journal-2511	136	27	on	on	ADP
american_scientific_journal-2511	136	28	this	this	DET
american_scientific_journal-2511	136	29	point	point	NOUN
american_scientific_journal-2511	136	30	because	because	SCONJ
american_scientific_journal-2511	136	31	using	use	VERB
american_scientific_journal-2511	136	32	of	of	ADP
american_scientific_journal-2511	136	33	leeches	leech	NOUN
american_scientific_journal-2511	136	34	directly	directly	ADV
american_scientific_journal-2511	136	35	in	in	ADP
american_scientific_journal-2511	136	36	the	the	DET
american_scientific_journal-2511	136	37	surgery	surgery	NOUN
american_scientific_journal-2511	136	38	may	may	AUX
american_scientific_journal-2511	136	39	lead	lead	VERB
american_scientific_journal-2511	136	40	to	to	ADP
american_scientific_journal-2511	136	41	infections	infection	NOUN
american_scientific_journal-2511	136	42	by	by	ADP
american_scientific_journal-2511	136	43	bacteria	bacteria	NOUN
american_scientific_journal-2511	136	44	in	in	ADP
american_scientific_journal-2511	136	45	its	its	PRON
american_scientific_journal-2511	136	46	digestive	digestive	ADJ
american_scientific_journal-2511	136	47	system	system	NOUN
american_scientific_journal-2511	136	48	because	because	SCONJ
american_scientific_journal-2511	136	49	of	of	ADP
american_scientific_journal-2511	136	50	that	that	DET
american_scientific_journal-2511	136	51	antibiotic	antibiotic	ADJ
american_scientific_journal-2511	136	52	treatment	treatment	NOUN
american_scientific_journal-2511	136	53	is	be	AUX
american_scientific_journal-2511	136	54	very	very	ADV
american_scientific_journal-2511	136	55	important	important	ADJ
american_scientific_journal-2511	136	56	step	step	NOUN
american_scientific_journal-2511	136	57	after	after	ADP
american_scientific_journal-2511	136	58	leech	leech	NOUN
american_scientific_journal-2511	136	59	therapy	therapy	NOUN
american_scientific_journal-2511	136	60	and	and	CCONJ
american_scientific_journal-2511	136	61	the	the	DET
american_scientific_journal-2511	136	62	type	type	NOUN
american_scientific_journal-2511	136	63	of	of	ADP
american_scientific_journal-2511	136	64	antibiotic	antibiotic	NOUN
american_scientific_journal-2511	136	65	used	use	VERB
american_scientific_journal-2511	136	66	in	in	ADP
american_scientific_journal-2511	136	67	this	this	DET
american_scientific_journal-2511	136	68	treatment	treatment	NOUN
american_scientific_journal-2511	136	69	depend	depend	VERB
american_scientific_journal-2511	136	70	on	on	ADP
american_scientific_journal-2511	136	71	the	the	DET
american_scientific_journal-2511	136	72	type	type	NOUN
american_scientific_journal-2511	136	73	of	of	ADP
american_scientific_journal-2511	136	74	bacteria	bacteria	NOUN
american_scientific_journal-2511	136	75	which	which	PRON
american_scientific_journal-2511	136	76	different	different	ADJ
american_scientific_journal-2511	136	77	depending	depend	VERB
american_scientific_journal-2511	136	78	on	on	ADP
american_scientific_journal-2511	136	79	the	the	DET
american_scientific_journal-2511	136	80	leeches	leech	NOUN
american_scientific_journal-2511	136	81	type	type	NOUN
american_scientific_journal-2511	136	82	[	[	X
american_scientific_journal-2511	136	83	17	17	NUM
american_scientific_journal-2511	136	84	]	]	PUNCT
american_scientific_journal-2511	136	85	.	.	PUNCT
american_scientific_journal-2511	137	1	in	in	ADP
american_scientific_journal-2511	137	2	this	this	DET
american_scientific_journal-2511	137	3	study	study	NOUN
american_scientific_journal-2511	137	4	the	the	DET
american_scientific_journal-2511	137	5	extraction	extraction	NOUN
american_scientific_journal-2511	137	6	process	process	NOUN
american_scientific_journal-2511	137	7	has	have	AUX
american_scientific_journal-2511	137	8	been	be	AUX
american_scientific_journal-2511	137	9	done	do	VERB
american_scientific_journal-2511	137	10	by	by	ADP
american_scientific_journal-2511	137	11	using	use	VERB
american_scientific_journal-2511	137	12	rneasy	rneasy	NOUN
american_scientific_journal-2511	137	13	plus	plus	CCONJ
american_scientific_journal-2511	137	14	mini	mini	ADJ
american_scientific_journal-2511	137	15	kit	kit	NOUN
american_scientific_journal-2511	137	16	from	from	ADP
american_scientific_journal-2511	137	17	qiagen	qiagen	PROPN
american_scientific_journal-2511	137	18	,	,	PUNCT
american_scientific_journal-2511	137	19	this	this	DET
american_scientific_journal-2511	137	20	kit	kit	NOUN
american_scientific_journal-2511	137	21	make	make	VERB
american_scientific_journal-2511	137	22	the	the	DET
american_scientific_journal-2511	137	23	rna	rna	NOUN
american_scientific_journal-2511	137	24	extraction	extraction	NOUN
american_scientific_journal-2511	137	25	easy	easy	ADJ
american_scientific_journal-2511	137	26	because	because	SCONJ
american_scientific_journal-2511	137	27	first	first	ADV
american_scientific_journal-2511	137	28	of	of	ADP
american_scientific_journal-2511	137	29	all	all	DET
american_scientific_journal-2511	137	30	the	the	DET
american_scientific_journal-2511	137	31	tissue	tissue	NOUN
american_scientific_journal-2511	137	32	sample	sample	NOUN
american_scientific_journal-2511	137	33	lysed	lyse	VERB
american_scientific_journal-2511	137	34	and	and	CCONJ
american_scientific_journal-2511	137	35	homogenized	homogenize	VERB
american_scientific_journal-2511	137	36	in	in	ADP
american_scientific_journal-2511	137	37	a	a	DET
american_scientific_journal-2511	137	38	highly	highly	ADV
american_scientific_journal-2511	137	39	denaturing	denature	VERB
american_scientific_journal-2511	137	40	guanidine	guanidine	NOUN
american_scientific_journal-2511	137	41	-	-	PUNCT
american_scientific_journal-2511	137	42	isothiocyanate	isothiocyanate	NOUN
american_scientific_journal-2511	137	43	–	–	PUNCT
american_scientific_journal-2511	137	44	containing	contain	VERB
american_scientific_journal-2511	137	45	buffer	buffer	NOUN
american_scientific_journal-2511	137	46	which	which	PRON
american_scientific_journal-2511	137	47	immediately	immediately	ADV
american_scientific_journal-2511	137	48	inactivates	inactivate	VERB
american_scientific_journal-2511	137	49	rnases	rnase	NOUN
american_scientific_journal-2511	137	50	to	to	PART
american_scientific_journal-2511	137	51	ensure	ensure	VERB
american_scientific_journal-2511	137	52	isolation	isolation	NOUN
american_scientific_journal-2511	137	53	of	of	ADP
american_scientific_journal-2511	137	54	intact	intact	ADJ
american_scientific_journal-2511	137	55	rna	rna	NOUN
american_scientific_journal-2511	137	56	,	,	PUNCT
american_scientific_journal-2511	137	57	the	the	DET
american_scientific_journal-2511	137	58	lysate	lysate	NOUN
american_scientific_journal-2511	137	59	is	be	AUX
american_scientific_journal-2511	137	60	then	then	ADV
american_scientific_journal-2511	137	61	passed	pass	VERB
american_scientific_journal-2511	137	62	through	through	ADP
american_scientific_journal-2511	137	63	a	a	DET
american_scientific_journal-2511	137	64	gdna	gdna	ADJ
american_scientific_journal-2511	137	65	eliminator	eliminator	NOUN
american_scientific_journal-2511	137	66	spin	spin	NOUN
american_scientific_journal-2511	137	67	column	column	NOUN
american_scientific_journal-2511	137	68	.	.	PUNCT
american_scientific_journal-2511	138	1	this	this	DET
american_scientific_journal-2511	138	2	column	column	NOUN
american_scientific_journal-2511	138	3	,	,	PUNCT
american_scientific_journal-2511	138	4	in	in	ADP
american_scientific_journal-2511	138	5	combination	combination	NOUN
american_scientific_journal-2511	138	6	with	with	ADP
american_scientific_journal-2511	138	7	the	the	DET
american_scientific_journal-2511	138	8	optimized	optimize	VERB
american_scientific_journal-2511	138	9	high	high	ADJ
american_scientific_journal-2511	138	10	-	-	PUNCT
american_scientific_journal-2511	138	11	salt	salt	NOUN
american_scientific_journal-2511	138	12	buffer	buffer	NOUN
american_scientific_journal-2511	138	13	,	,	PUNCT
american_scientific_journal-2511	138	14	allows	allow	VERB
american_scientific_journal-2511	138	15	efficient	efficient	ADJ
american_scientific_journal-2511	138	16	removal	removal	NOUN
american_scientific_journal-2511	138	17	of	of	ADP
american_scientific_journal-2511	138	18	genomic	genomic	ADJ
american_scientific_journal-2511	138	19	dna	dna	PROPN
american_scientific_journal-2511	138	20	.	.	PUNCT
american_scientific_journal-2511	139	1	the	the	DET
american_scientific_journal-2511	139	2	integrity	integrity	NOUN
american_scientific_journal-2511	139	3	of	of	ADP
american_scientific_journal-2511	139	4	rna	rna	PROPN
american_scientific_journal-2511	139	5	is	be	AUX
american_scientific_journal-2511	139	6	best	well	ADV
american_scientific_journal-2511	139	7	determined	determine	VERB
american_scientific_journal-2511	139	8	by	by	ADP
american_scientific_journal-2511	139	9	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	139	10	as	as	SCONJ
american_scientific_journal-2511	139	11	in	in	ADP
american_scientific_journal-2511	139	12	figure	figure	NOUN
american_scientific_journal-2511	139	13	3	3	NUM
american_scientific_journal-2511	139	14	rna	rna	NOUN
american_scientific_journal-2511	139	15	was	be	AUX
american_scientific_journal-2511	139	16	separated	separate	VERB
american_scientific_journal-2511	139	17	as	as	ADP
american_scientific_journal-2511	139	18	distinct	distinct	ADJ
american_scientific_journal-2511	139	19	band	band	NOUN
american_scientific_journal-2511	139	20	on	on	ADP
american_scientific_journal-2511	139	21	agarose	agarose	NOUN
american_scientific_journal-2511	139	22	gel	gel	NOUN
american_scientific_journal-2511	139	23	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	139	24	of	of	ADP
american_scientific_journal-2511	139	25	about	about	ADV
american_scientific_journal-2511	139	26	1200	1200	NUM
american_scientific_journal-2511	139	27	bp	bp	PROPN
american_scientific_journal-2511	139	28	and	and	CCONJ
american_scientific_journal-2511	139	29	this	this	DET
american_scientific_journal-2511	139	30	result	result	NOUN
american_scientific_journal-2511	139	31	compatible	compatible	ADJ
american_scientific_journal-2511	139	32	with	with	ADP
american_scientific_journal-2511	139	33	rneasy	rneasy	NOUN
american_scientific_journal-2511	139	34	plus	plus	CCONJ
american_scientific_journal-2511	139	35	kit	kit	NOUN
american_scientific_journal-2511	139	36	recommendation	recommendation	NOUN
american_scientific_journal-2511	139	37	which	which	PRON
american_scientific_journal-2511	139	38	is	be	AUX
american_scientific_journal-2511	139	39	all	all	DET
american_scientific_journal-2511	139	40	rna	rna	NOUN
american_scientific_journal-2511	139	41	molecules	molecule	NOUN
american_scientific_journal-2511	139	42	longer	long	ADV
american_scientific_journal-2511	139	43	than	than	SCONJ
american_scientific_journal-2511	139	44	200	200	NUM
american_scientific_journal-2511	139	45	nucleotides	nucleotide	NOUN
american_scientific_journal-2511	139	46	are	be	AUX
american_scientific_journal-2511	139	47	isolated	isolate	VERB
american_scientific_journal-2511	139	48	.	.	PUNCT
american_scientific_journal-2511	140	1	the	the	DET
american_scientific_journal-2511	140	2	procedure	procedure	NOUN
american_scientific_journal-2511	140	3	provides	provide	VERB
american_scientific_journal-2511	140	4	an	an	DET
american_scientific_journal-2511	140	5	enrichment	enrichment	NOUN
american_scientific_journal-2511	140	6	for	for	ADP
american_scientific_journal-2511	140	7	mrna	mrna	PROPN
american_scientific_journal-2511	140	8	since	since	SCONJ
american_scientific_journal-2511	140	9	most	most	ADJ
american_scientific_journal-2511	140	10	rnas	rna	NOUN
american_scientific_journal-2511	140	11	<	<	X
american_scientific_journal-2511	140	12	200	200	NUM
american_scientific_journal-2511	140	13	nucleotides	nucleotide	NOUN
american_scientific_journal-2511	140	14	(	(	PUNCT
american_scientific_journal-2511	140	15	such	such	ADJ
american_scientific_journal-2511	140	16	as	as	ADP
american_scientific_journal-2511	140	17	5.8s	5.8s	PROPN
american_scientific_journal-2511	140	18	rrna	rrna	PROPN
american_scientific_journal-2511	140	19	,	,	PUNCT
american_scientific_journal-2511	140	20	5s	5s	NUM
american_scientific_journal-2511	140	21	rrna	rrna	NOUN
american_scientific_journal-2511	140	22	,	,	PUNCT
american_scientific_journal-2511	140	23	and	and	CCONJ
american_scientific_journal-2511	140	24	trnas	trnas	NOUN
american_scientific_journal-2511	140	25	which	which	PRON
american_scientific_journal-2511	140	26	together	together	ADV
american_scientific_journal-2511	140	27	comprise	comprise	VERB
american_scientific_journal-2511	140	28	15–20	15–20	NUM
american_scientific_journal-2511	140	29	%	%	NOUN
american_scientific_journal-2511	140	30	of	of	ADP
american_scientific_journal-2511	140	31	total	total	ADJ
american_scientific_journal-2511	140	32	rna	rna	NOUN
american_scientific_journal-2511	140	33	)	)	PUNCT
american_scientific_journal-2511	140	34	are	be	AUX
american_scientific_journal-2511	140	35	selectively	selectively	ADV
american_scientific_journal-2511	140	36	excluded	exclude	VERB
american_scientific_journal-2511	140	37	[	[	PUNCT
american_scientific_journal-2511	140	38	18	18	NUM
american_scientific_journal-2511	140	39	]	]	PUNCT
american_scientific_journal-2511	140	40	.	.	PUNCT
american_scientific_journal-2511	141	1	two	two	NUM
american_scientific_journal-2511	141	2	types	type	NOUN
american_scientific_journal-2511	141	3	of	of	ADP
american_scientific_journal-2511	141	4	competent	competent	ADJ
american_scientific_journal-2511	141	5	genetic	genetic	ADJ
american_scientific_journal-2511	141	6	engineering	engineering	NOUN
american_scientific_journal-2511	141	7	strains	strain	NOUN
american_scientific_journal-2511	141	8	,	,	PUNCT
american_scientific_journal-2511	141	9	dh5α	dh5α	PROPN
american_scientific_journal-2511	141	10	and	and	CCONJ
american_scientific_journal-2511	141	11	bl21(de3	bl21(de3	VERB
american_scientific_journal-2511	141	12	)	)	PUNCT
american_scientific_journal-2511	141	13	were	be	AUX
american_scientific_journal-2511	141	14	transformed	transform	VERB
american_scientific_journal-2511	141	15	with	with	ADP
american_scientific_journal-2511	141	16	constructive	constructive	ADJ
american_scientific_journal-2511	141	17	vector	vector	NOUN
american_scientific_journal-2511	141	18	pet-16b	pet-16b	NOUN
american_scientific_journal-2511	141	19	-	-	PUNCT
american_scientific_journal-2511	141	20	hr	hr	NOUN
american_scientific_journal-2511	141	21	,	,	PUNCT
american_scientific_journal-2511	141	22	figure	figure	NOUN
american_scientific_journal-2511	141	23	6	6	NUM
american_scientific_journal-2511	141	24	(	(	PUNCT
american_scientific_journal-2511	141	25	a	a	PRON
american_scientific_journal-2511	141	26	&	&	CCONJ
american_scientific_journal-2511	141	27	b	b	NOUN
american_scientific_journal-2511	141	28	)	)	PUNCT
american_scientific_journal-2511	141	29	.	.	PUNCT
american_scientific_journal-2511	142	1	dh5α	dh5α	PROPN
american_scientific_journal-2511	142	2	strain	strain	NOUN
american_scientific_journal-2511	142	3	these	these	DET
american_scientific_journal-2511	142	4	cells	cell	NOUN
american_scientific_journal-2511	142	5	are	be	AUX
american_scientific_journal-2511	142	6	compatible	compatible	ADJ
american_scientific_journal-2511	142	7	with	with	ADP
american_scientific_journal-2511	142	8	lacz	lacz	NOUN
american_scientific_journal-2511	142	9	blue	blue	ADJ
american_scientific_journal-2511	142	10	/	/	SYM
american_scientific_journal-2511	142	11	white	white	ADJ
american_scientific_journal-2511	142	12	selection	selection	NOUN
american_scientific_journal-2511	142	13	procedures	procedure	NOUN
american_scientific_journal-2511	142	14	,	,	PUNCT
american_scientific_journal-2511	142	15	may	may	AUX
american_scientific_journal-2511	142	16	contain	contain	VERB
american_scientific_journal-2511	142	17	color	color	NOUN
american_scientific_journal-2511	142	18	selection	selection	NOUN
american_scientific_journal-2511	142	19	markers	marker	NOUN
american_scientific_journal-2511	142	20	which	which	PRON
american_scientific_journal-2511	142	21	provide	provide	VERB
american_scientific_journal-2511	142	22	blue	blue	ADJ
american_scientific_journal-2511	142	23	/	/	SYM
american_scientific_journal-2511	142	24	white	white	ADJ
american_scientific_journal-2511	142	25	screening	screening	NOUN
american_scientific_journal-2511	142	26	(	(	PUNCT
american_scientific_journal-2511	142	27	via	via	ADP
american_scientific_journal-2511	142	28	αfactor	αfactor	NOUN
american_scientific_journal-2511	142	29	american	american	PROPN
american_scientific_journal-2511	142	30	scientific	scientific	PROPN
american_scientific_journal-2511	142	31	research	research	PROPN
american_scientific_journal-2511	142	32	journal	journal	NOUN
american_scientific_journal-2511	142	33	for	for	ADP
american_scientific_journal-2511	142	34	engineering	engineering	NOUN
american_scientific_journal-2511	142	35	,	,	PUNCT
american_scientific_journal-2511	142	36	technology	technology	NOUN
american_scientific_journal-2511	142	37	,	,	PUNCT
american_scientific_journal-2511	142	38	and	and	CCONJ
american_scientific_journal-2511	142	39	sciences	science	NOUN
american_scientific_journal-2511	142	40	(	(	PUNCT
american_scientific_journal-2511	142	41	asrjets	asrjet	NOUN
american_scientific_journal-2511	142	42	)	)	PUNCT
american_scientific_journal-2511	142	43	(	(	PUNCT
american_scientific_journal-2511	142	44	2017	2017	NUM
american_scientific_journal-2511	142	45	)	)	PUNCT
american_scientific_journal-2511	142	46	volume	volume	NOUN
american_scientific_journal-2511	142	47	27	27	NUM
american_scientific_journal-2511	142	48	,	,	PUNCT
american_scientific_journal-2511	142	49	no	no	DET
american_scientific_journal-2511	142	50	1	1	NUM
american_scientific_journal-2511	142	51	,	,	PUNCT
american_scientific_journal-2511	142	52	pp	pp	ADV
american_scientific_journal-2511	142	53	25	25	NUM
american_scientific_journal-2511	142	54	-	-	SYM
american_scientific_journal-2511	142	55	42	42	NUM
american_scientific_journal-2511	142	56	38	38	NUM
american_scientific_journal-2511	142	57	complementation	complementation	NOUN
american_scientific_journal-2511	142	58	)	)	PUNCT
american_scientific_journal-2511	142	59	,	,	PUNCT
american_scientific_journal-2511	142	60	on	on	ADP
american_scientific_journal-2511	142	61	x	x	ADJ
american_scientific_journal-2511	142	62	-	-	ADJ
american_scientific_journal-2511	142	63	gal	gal	ADJ
american_scientific_journal-2511	142	64	medium	medium	NOUN
american_scientific_journal-2511	142	65	the	the	DET
american_scientific_journal-2511	142	66	molecular	molecular	ADJ
american_scientific_journal-2511	142	67	mechanism	mechanism	NOUN
american_scientific_journal-2511	142	68	for	for	ADP
american_scientific_journal-2511	142	69	blue	blue	ADJ
american_scientific_journal-2511	142	70	/	/	SYM
american_scientific_journal-2511	142	71	white	white	ADJ
american_scientific_journal-2511	142	72	screening	screening	NOUN
american_scientific_journal-2511	142	73	is	be	AUX
american_scientific_journal-2511	142	74	based	base	VERB
american_scientific_journal-2511	142	75	on	on	ADP
american_scientific_journal-2511	142	76	a	a	DET
american_scientific_journal-2511	142	77	genetic	genetic	ADJ
american_scientific_journal-2511	142	78	engineering	engineering	NOUN
american_scientific_journal-2511	142	79	of	of	ADP
american_scientific_journal-2511	142	80	the	the	DET
american_scientific_journal-2511	142	81	lac	lac	PROPN
american_scientific_journal-2511	142	82	operon	operon	PROPN
american_scientific_journal-2511	142	83	in	in	ADP
american_scientific_journal-2511	142	84	the	the	DET
american_scientific_journal-2511	142	85	e.	e.	PROPN
american_scientific_journal-2511	142	86	coli	coli	NOUN
american_scientific_journal-2511	142	87	as	as	ADP
american_scientific_journal-2511	142	88	a	a	DET
american_scientific_journal-2511	142	89	host	host	NOUN
american_scientific_journal-2511	142	90	cell	cell	NOUN
american_scientific_journal-2511	142	91	combined	combine	VERB
american_scientific_journal-2511	142	92	with	with	ADP
american_scientific_journal-2511	142	93	a	a	DET
american_scientific_journal-2511	142	94	subunit	subunit	NOUN
american_scientific_journal-2511	142	95	complementation	complementation	NOUN
american_scientific_journal-2511	142	96	achieved	achieve	VERB
american_scientific_journal-2511	142	97	with	with	ADP
american_scientific_journal-2511	142	98	the	the	DET
american_scientific_journal-2511	142	99	cloning	cloning	NOUN
american_scientific_journal-2511	142	100	vector	vector	NOUN
american_scientific_journal-2511	142	101	.	.	PUNCT
american_scientific_journal-2511	143	1	in	in	ADP
american_scientific_journal-2511	143	2	the	the	DET
american_scientific_journal-2511	143	3	case	case	NOUN
american_scientific_journal-2511	143	4	when	when	SCONJ
american_scientific_journal-2511	143	5	this	this	DET
american_scientific_journal-2511	143	6	complementation	complementation	NOUN
american_scientific_journal-2511	143	7	between	between	ADP
american_scientific_journal-2511	143	8	vector	vector	NOUN
american_scientific_journal-2511	143	9	and	and	CCONJ
american_scientific_journal-2511	143	10	strain	strain	NOUN
american_scientific_journal-2511	143	11	present	present	ADJ
american_scientific_journal-2511	143	12	in	in	ADP
american_scientific_journal-2511	143	13	the	the	DET
american_scientific_journal-2511	143	14	presence	presence	NOUN
american_scientific_journal-2511	143	15	of	of	ADP
american_scientific_journal-2511	143	16	iptg	iptg	NOUN
american_scientific_journal-2511	143	17	(	(	PUNCT
american_scientific_journal-2511	143	18	isopropyl	isopropyl	NOUN
american_scientific_journal-2511	143	19	β	β	NOUN
american_scientific_journal-2511	143	20	-	-	PUNCT
american_scientific_journal-2511	143	21	d-1	d-1	NOUN
american_scientific_journal-2511	143	22	-	-	PUNCT
american_scientific_journal-2511	143	23	thiogalactopyranoside	thiogalactopyranoside	NOUN
american_scientific_journal-2511	143	24	)	)	PUNCT
american_scientific_journal-2511	143	25	as	as	ADP
american_scientific_journal-2511	143	26	an	an	DET
american_scientific_journal-2511	143	27	inducer	inducer	NOUN
american_scientific_journal-2511	143	28	for	for	ADP
american_scientific_journal-2511	143	29	lac	lac	PROPN
american_scientific_journal-2511	143	30	operon	operon	PROPN
american_scientific_journal-2511	143	31	and	and	CCONJ
american_scientific_journal-2511	143	32	x	x	NOUN
american_scientific_journal-2511	143	33	-	-	NOUN
american_scientific_journal-2511	143	34	gal	gal	NOUN
american_scientific_journal-2511	143	35	as	as	ADP
american_scientific_journal-2511	143	36	an	an	DET
american_scientific_journal-2511	143	37	indicator	indicator	NOUN
american_scientific_journal-2511	143	38	which	which	PRON
american_scientific_journal-2511	143	39	is	be	AUX
american_scientific_journal-2511	143	40	a	a	DET
american_scientific_journal-2511	143	41	colorless	colorless	ADV
american_scientific_journal-2511	143	42	modified	modify	VERB
american_scientific_journal-2511	143	43	galactose	galactose	NOUN
american_scientific_journal-2511	143	44	sugar	sugar	NOUN
american_scientific_journal-2511	143	45	that	that	PRON
american_scientific_journal-2511	143	46	is	be	AUX
american_scientific_journal-2511	143	47	metabolized	metabolize	VERB
american_scientific_journal-2511	143	48	by	by	ADP
american_scientific_journal-2511	143	49	β	β	NOUN
american_scientific_journal-2511	143	50	-	-	NOUN
american_scientific_journal-2511	143	51	galactosidase	galactosidase	NOUN
american_scientific_journal-2511	143	52	(	(	PUNCT
american_scientific_journal-2511	143	53	a	a	DET
american_scientific_journal-2511	143	54	functional	functional	ADJ
american_scientific_journal-2511	143	55	enzyme	enzyme	NOUN
american_scientific_journal-2511	143	56	form	form	NOUN
american_scientific_journal-2511	143	57	upon	upon	SCONJ
american_scientific_journal-2511	143	58	complementation	complementation	NOUN
american_scientific_journal-2511	143	59	)	)	PUNCT
american_scientific_journal-2511	143	60	to	to	PART
american_scientific_journal-2511	143	61	form	form	VERB
american_scientific_journal-2511	143	62	5	5	NUM
american_scientific_journal-2511	143	63	-	-	PUNCT
american_scientific_journal-2511	143	64	bromo-4	bromo-4	NOUN
american_scientific_journal-2511	143	65	-	-	NOUN
american_scientific_journal-2511	143	66	chloroindoxyl	chloroindoxyl	NOUN
american_scientific_journal-2511	143	67	which	which	PRON
american_scientific_journal-2511	143	68	is	be	AUX
american_scientific_journal-2511	143	69	spontaneously	spontaneously	ADV
american_scientific_journal-2511	143	70	oxidized	oxidize	VERB
american_scientific_journal-2511	143	71	to	to	ADP
american_scientific_journal-2511	143	72	the	the	DET
american_scientific_journal-2511	143	73	bright	bright	ADJ
american_scientific_journal-2511	143	74	blue	blue	ADJ
american_scientific_journal-2511	143	75	insoluble	insoluble	ADJ
american_scientific_journal-2511	143	76	pigment	pigment	NOUN
american_scientific_journal-2511	143	77	5,5´-dibromo-4,4´-dichloro	5,5´-dibromo-4,4´-dichloro	NUM
american_scientific_journal-2511	143	78	-	-	PUNCT
american_scientific_journal-2511	143	79	indigo	indigo	NOUN
american_scientific_journal-2511	143	80	and	and	CCONJ
american_scientific_journal-2511	143	81	thus	thus	ADV
american_scientific_journal-2511	143	82	function	function	NOUN
american_scientific_journal-2511	143	83	as	as	ADP
american_scientific_journal-2511	143	84	an	an	DET
american_scientific_journal-2511	143	85	indicator	indicator	NOUN
american_scientific_journal-2511	143	86	,	,	PUNCT
american_scientific_journal-2511	143	87	because	because	SCONJ
american_scientific_journal-2511	143	88	pet-16b	pet-16b	NUM
american_scientific_journal-2511	143	89	vector	vector	NOUN
american_scientific_journal-2511	143	90	lack	lack	VERB
american_scientific_journal-2511	143	91	the	the	DET
american_scientific_journal-2511	143	92	complementation	complementation	NOUN
american_scientific_journal-2511	143	93	with	with	ADP
american_scientific_journal-2511	143	94	dh5α	dh5α	PROPN
american_scientific_journal-2511	143	95	strain	strain	NOUN
american_scientific_journal-2511	143	96	the	the	DET
american_scientific_journal-2511	143	97	colony	colony	NOUN
american_scientific_journal-2511	143	98	remain	remain	VERB
american_scientific_journal-2511	143	99	white	white	ADJ
american_scientific_journal-2511	143	100	because	because	SCONJ
american_scientific_journal-2511	143	101	of	of	ADP
american_scientific_journal-2511	143	102	that	that	DET
american_scientific_journal-2511	143	103	addition	addition	NOUN
american_scientific_journal-2511	143	104	antibiotic	antibiotic	ADJ
american_scientific_journal-2511	143	105	(	(	PUNCT
american_scientific_journal-2511	143	106	ampicillin	ampicillin	NOUN
american_scientific_journal-2511	143	107	)	)	PUNCT
american_scientific_journal-2511	143	108	to	to	ADP
american_scientific_journal-2511	143	109	the	the	DET
american_scientific_journal-2511	143	110	medium	medium	NOUN
american_scientific_journal-2511	143	111	is	be	AUX
american_scientific_journal-2511	143	112	very	very	ADV
american_scientific_journal-2511	143	113	important	important	ADJ
american_scientific_journal-2511	143	114	step	step	NOUN
american_scientific_journal-2511	143	115	because	because	SCONJ
american_scientific_journal-2511	143	116	dh5α	dh5α	PROPN
american_scientific_journal-2511	143	117	strain	strain	NOUN
american_scientific_journal-2511	143	118	sensitive	sensitive	ADJ
american_scientific_journal-2511	143	119	to	to	ADP
american_scientific_journal-2511	143	120	the	the	DET
american_scientific_journal-2511	143	121	ampicillin	ampicillin	NOUN
american_scientific_journal-2511	143	122	and	and	CCONJ
american_scientific_journal-2511	143	123	vector	vector	NOUN
american_scientific_journal-2511	143	124	include	include	VERB
american_scientific_journal-2511	143	125	this	this	DET
american_scientific_journal-2511	143	126	selective	selective	ADJ
american_scientific_journal-2511	143	127	marker	marker	NOUN
american_scientific_journal-2511	143	128	(	(	PUNCT
american_scientific_journal-2511	143	129	ampicillin	ampicillin	NOUN
american_scientific_journal-2511	143	130	resistance	resistance	NOUN
american_scientific_journal-2511	143	131	)	)	PUNCT
american_scientific_journal-2511	143	132	this	this	DET
american_scientific_journal-2511	143	133	selection	selection	NOUN
american_scientific_journal-2511	143	134	step	step	NOUN
american_scientific_journal-2511	143	135	give	give	VERB
american_scientific_journal-2511	143	136	evidence	evidence	NOUN
american_scientific_journal-2511	143	137	that	that	SCONJ
american_scientific_journal-2511	143	138	the	the	DET
american_scientific_journal-2511	143	139	ability	ability	NOUN
american_scientific_journal-2511	143	140	of	of	ADP
american_scientific_journal-2511	143	141	this	this	DET
american_scientific_journal-2511	143	142	cells	cell	NOUN
american_scientific_journal-2511	143	143	grown	grow	VERB
american_scientific_journal-2511	143	144	on	on	ADP
american_scientific_journal-2511	143	145	the	the	DET
american_scientific_journal-2511	143	146	medium	medium	ADJ
american_scientific_journal-2511	143	147	contain	contain	NOUN
american_scientific_journal-2511	143	148	ampicillin	ampicillin	NOUN
american_scientific_journal-2511	143	149	due	due	ADP
american_scientific_journal-2511	143	150	to	to	ADP
american_scientific_journal-2511	143	151	harboring	harbor	VERB
american_scientific_journal-2511	143	152	pet-16b	pet-16b	NUM
american_scientific_journal-2511	143	153	vector	vector	NOUN
american_scientific_journal-2511	143	154	,	,	PUNCT
american_scientific_journal-2511	143	155	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2511	143	156	)	)	PUNCT
american_scientific_journal-2511	143	157	strain	strain	NOUN
american_scientific_journal-2511	143	158	also	also	ADV
american_scientific_journal-2511	143	159	sensitive	sensitive	ADJ
american_scientific_journal-2511	143	160	to	to	ADP
american_scientific_journal-2511	143	161	the	the	DET
american_scientific_journal-2511	143	162	ampicillin	ampicillin	NOUN
american_scientific_journal-2511	143	163	while	while	SCONJ
american_scientific_journal-2511	143	164	pet-16b	pet-16b	NUM
american_scientific_journal-2511	143	165	vector	vector	NOUN
american_scientific_journal-2511	143	166	carry	carry	VERB
american_scientific_journal-2511	143	167	ampicillin	ampicillin	NOUN
american_scientific_journal-2511	143	168	resistance	resistance	NOUN
american_scientific_journal-2511	143	169	as	as	ADP
american_scientific_journal-2511	143	170	selective	selective	ADJ
american_scientific_journal-2511	143	171	marker	marker	NOUN
american_scientific_journal-2511	143	172	because	because	SCONJ
american_scientific_journal-2511	143	173	of	of	ADP
american_scientific_journal-2511	143	174	that	that	SCONJ
american_scientific_journal-2511	143	175	the	the	DET
american_scientific_journal-2511	143	176	addition	addition	NOUN
american_scientific_journal-2511	143	177	of	of	ADP
american_scientific_journal-2511	143	178	ampicillin	ampicillin	NOUN
american_scientific_journal-2511	143	179	to	to	ADP
american_scientific_journal-2511	143	180	the	the	DET
american_scientific_journal-2511	143	181	medium	medium	NOUN
american_scientific_journal-2511	143	182	and	and	CCONJ
american_scientific_journal-2511	143	183	the	the	DET
american_scientific_journal-2511	143	184	ability	ability	NOUN
american_scientific_journal-2511	143	185	of	of	ADP
american_scientific_journal-2511	143	186	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2511	143	187	)	)	PUNCT
american_scientific_journal-2511	143	188	strain	strain	NOUN
american_scientific_journal-2511	143	189	grown	grow	VERB
american_scientific_journal-2511	143	190	on	on	ADP
american_scientific_journal-2511	143	191	it	it	PRON
american_scientific_journal-2511	143	192	due	due	ADP
american_scientific_journal-2511	143	193	to	to	ADP
american_scientific_journal-2511	143	194	harboaring	harboaring	NOUN
american_scientific_journal-2511	143	195	pet-16b	pet-16b	NUM
american_scientific_journal-2511	143	196	plasmid	plasmid	ADJ
american_scientific_journal-2511	143	197	[	[	X
american_scientific_journal-2511	143	198	19	19	NUM
american_scientific_journal-2511	143	199	]	]	PUNCT
american_scientific_journal-2511	143	200	.	.	PUNCT
american_scientific_journal-2511	144	1	bl21(de3	bl21(de3	VERB
american_scientific_journal-2511	144	2	)	)	PUNCT
american_scientific_journal-2511	144	3	strain	strain	NOUN
american_scientific_journal-2511	144	4	is	be	AUX
american_scientific_journal-2511	144	5	one	one	NUM
american_scientific_journal-2511	144	6	of	of	ADP
american_scientific_journal-2511	144	7	the	the	DET
american_scientific_journal-2511	144	8	widely	widely	ADV
american_scientific_journal-2511	144	9	-	-	PUNCT
american_scientific_journal-2511	144	10	used	use	VERB
american_scientific_journal-2511	144	11	strain	strain	NOUN
american_scientific_journal-2511	144	12	to	to	PART
american_scientific_journal-2511	144	13	check	check	VERB
american_scientific_journal-2511	144	14	the	the	DET
american_scientific_journal-2511	144	15	basic	basic	ADJ
american_scientific_journal-2511	144	16	protein	protein	NOUN
american_scientific_journal-2511	144	17	expression	expression	NOUN
american_scientific_journal-2511	144	18	in	in	ADP
american_scientific_journal-2511	144	19	e.	e.	PROPN
american_scientific_journal-2511	144	20	coli	coli	PROPN
american_scientific_journal-2511	144	21	.	.	PUNCT
american_scientific_journal-2511	145	1	chromosomal	chromosomal	ADJ
american_scientific_journal-2511	145	2	de3	de3	PROPN
american_scientific_journal-2511	145	3	prophase	prophase	PROPN
american_scientific_journal-2511	145	4	expresses	express	VERB
american_scientific_journal-2511	145	5	t7	t7	PROPN
american_scientific_journal-2511	145	6	rna	rna	PROPN
american_scientific_journal-2511	145	7	polymerase	polymerase	NOUN
american_scientific_journal-2511	145	8	under	under	ADP
american_scientific_journal-2511	145	9	control	control	NOUN
american_scientific_journal-2511	145	10	of	of	ADP
american_scientific_journal-2511	145	11	lac	lac	PROPN
american_scientific_journal-2511	145	12	promoter	promoter	NOUN
american_scientific_journal-2511	145	13	also	also	ADV
american_scientific_journal-2511	145	14	this	this	DET
american_scientific_journal-2511	145	15	strain	strain	NOUN
american_scientific_journal-2511	145	16	lack	lack	NOUN
american_scientific_journal-2511	145	17	lon	lon	NOUN
american_scientific_journal-2511	145	18	and	and	CCONJ
american_scientific_journal-2511	145	19	ompt	ompt	NOUN
american_scientific_journal-2511	145	20	proteases	protease	NOUN
american_scientific_journal-2511	145	21	which	which	PRON
american_scientific_journal-2511	145	22	will	will	AUX
american_scientific_journal-2511	145	23	stabilize	stabilize	VERB
american_scientific_journal-2511	145	24	expression	expression	NOUN
american_scientific_journal-2511	145	25	of	of	ADP
american_scientific_journal-2511	145	26	some	some	DET
american_scientific_journal-2511	145	27	recombinant	recombinant	ADJ
american_scientific_journal-2511	145	28	proteins	protein	NOUN
american_scientific_journal-2511	145	29	,	,	PUNCT
american_scientific_journal-2511	145	30	this	this	DET
american_scientific_journal-2511	145	31	strain	strain	NOUN
american_scientific_journal-2511	145	32	form	form	VERB
american_scientific_journal-2511	145	33	a	a	DET
american_scientific_journal-2511	145	34	compatible	compatible	ADJ
american_scientific_journal-2511	145	35	host	host	NOUN
american_scientific_journal-2511	145	36	for	for	ADP
american_scientific_journal-2511	145	37	pet	pet	ADJ
american_scientific_journal-2511	145	38	vector	vector	NOUN
american_scientific_journal-2511	145	39	,	,	PUNCT
american_scientific_journal-2511	145	40	constructive	constructive	ADJ
american_scientific_journal-2511	145	41	vector	vector	NOUN
american_scientific_journal-2511	145	42	transferred	transfer	VERB
american_scientific_journal-2511	145	43	to	to	ADP
american_scientific_journal-2511	145	44	the	the	DET
american_scientific_journal-2511	145	45	host	host	NOUN
american_scientific_journal-2511	145	46	strain	strain	NOUN
american_scientific_journal-2511	145	47	[	[	X
american_scientific_journal-2511	145	48	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2511	145	49	)	)	PUNCT
american_scientific_journal-2511	145	50	]	]	PUNCT
american_scientific_journal-2511	145	51	,	,	PUNCT
american_scientific_journal-2511	145	52	pet	pet	ADJ
american_scientific_journal-2511	145	53	vector	vector	NOUN
american_scientific_journal-2511	145	54	contain	contain	NOUN
american_scientific_journal-2511	145	55	t7	t7	PROPN
american_scientific_journal-2511	145	56	promoter	promoter	NOUN
american_scientific_journal-2511	145	57	which	which	PRON
american_scientific_journal-2511	145	58	is	be	AUX
american_scientific_journal-2511	145	59	strong	strong	ADJ
american_scientific_journal-2511	145	60	promoter	promoter	NOUN
american_scientific_journal-2511	145	61	produced	produce	VERB
american_scientific_journal-2511	145	62	their	their	PRON
american_scientific_journal-2511	145	63	own	own	ADJ
american_scientific_journal-2511	145	64	rna	rna	NOUN
american_scientific_journal-2511	145	65	polymerase	polymerase	NOUN
american_scientific_journal-2511	145	66	[	[	PUNCT
american_scientific_journal-2511	145	67	introduced	introduce	VERB
american_scientific_journal-2511	145	68	into	into	ADP
american_scientific_journal-2511	145	69	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2511	145	70	)	)	PUNCT
american_scientific_journal-2511	145	71	]	]	PUNCT
american_scientific_journal-2511	145	72	under	under	ADP
american_scientific_journal-2511	145	73	the	the	DET
american_scientific_journal-2511	145	74	control	control	NOUN
american_scientific_journal-2511	145	75	of	of	ADP
american_scientific_journal-2511	145	76	lac	lac	PROPN
american_scientific_journal-2511	145	77	promoter	promoter	NOUN
american_scientific_journal-2511	145	78	,	,	PUNCT
american_scientific_journal-2511	145	79	as	as	ADV
american_scientific_journal-2511	145	80	long	long	ADV
american_scientific_journal-2511	145	81	as	as	SCONJ
american_scientific_journal-2511	145	82	t7	t7	PROPN
american_scientific_journal-2511	145	83	polymerase	polymerase	NOUN
american_scientific_journal-2511	145	84	is	be	AUX
american_scientific_journal-2511	145	85	a	a	DET
american_scientific_journal-2511	145	86	highly	highly	ADV
american_scientific_journal-2511	145	87	selective	selective	ADJ
american_scientific_journal-2511	145	88	enzyme	enzyme	NOUN
american_scientific_journal-2511	145	89	because	because	SCONJ
american_scientific_journal-2511	145	90	of	of	ADP
american_scientific_journal-2511	145	91	that	that	PRON
american_scientific_journal-2511	145	92	when	when	SCONJ
american_scientific_journal-2511	145	93	inserted	insert	VERB
american_scientific_journal-2511	145	94	plasmid	plasmid	NOUN
american_scientific_journal-2511	145	95	with	with	ADP
american_scientific_journal-2511	145	96	t7	t7	PROPN
american_scientific_journal-2511	145	97	promoter	promoter	NOUN
american_scientific_journal-2511	145	98	into	into	ADP
american_scientific_journal-2511	145	99	strain	strain	NOUN
american_scientific_journal-2511	145	100	without	without	ADP
american_scientific_journal-2511	145	101	t7	t7	PROPN
american_scientific_journal-2511	145	102	polymerase	polymerase	NOUN
american_scientific_journal-2511	145	103	the	the	DET
american_scientific_journal-2511	145	104	result	result	NOUN
american_scientific_journal-2511	145	105	is	be	AUX
american_scientific_journal-2511	145	106	no	no	DET
american_scientific_journal-2511	145	107	expression	expression	NOUN
american_scientific_journal-2511	145	108	,	,	PUNCT
american_scientific_journal-2511	145	109	alsot7	alsot7	NOUN
american_scientific_journal-2511	145	110	promoter	promoter	NOUN
american_scientific_journal-2511	145	111	is	be	AUX
american_scientific_journal-2511	145	112	under	under	ADP
american_scientific_journal-2511	145	113	the	the	DET
american_scientific_journal-2511	145	114	control	control	NOUN
american_scientific_journal-2511	145	115	of	of	ADP
american_scientific_journal-2511	145	116	lac	lac	PROPN
american_scientific_journal-2511	145	117	promoter	promoter	NOUN
american_scientific_journal-2511	145	118	,	,	PUNCT
american_scientific_journal-2511	145	119	if	if	SCONJ
american_scientific_journal-2511	145	120	iptg	iptg	PROPN
american_scientific_journal-2511	145	121	is	be	AUX
american_scientific_journal-2511	145	122	added	add	VERB
american_scientific_journal-2511	145	123	to	to	ADP
american_scientific_journal-2511	145	124	the	the	DET
american_scientific_journal-2511	145	125	culture	culture	NOUN
american_scientific_journal-2511	145	126	,	,	PUNCT
american_scientific_journal-2511	145	127	the	the	DET
american_scientific_journal-2511	145	128	lac	lac	PROPN
american_scientific_journal-2511	145	129	promoter	promoter	NOUN
american_scientific_journal-2511	145	130	is	be	AUX
american_scientific_journal-2511	145	131	derepressed	derepresse	VERB
american_scientific_journal-2511	145	132	t7	t7	PROPN
american_scientific_journal-2511	145	133	rna	rna	PROPN
american_scientific_journal-2511	145	134	polymerase	polymerase	NOUN
american_scientific_journal-2511	145	135	is	be	AUX
american_scientific_journal-2511	145	136	synthesized	synthesize	VERB
american_scientific_journal-2511	145	137	,	,	PUNCT
american_scientific_journal-2511	145	138	and	and	CCONJ
american_scientific_journal-2511	145	139	the	the	DET
american_scientific_journal-2511	145	140	gene	gene	NOUN
american_scientific_journal-2511	145	141	is	be	AUX
american_scientific_journal-2511	145	142	highly	highly	ADV
american_scientific_journal-2511	145	143	expressed	express	VERB
american_scientific_journal-2511	145	144	,	,	PUNCT
american_scientific_journal-2511	145	145	expression	expression	NOUN
american_scientific_journal-2511	145	146	vectors	vector	NOUN
american_scientific_journal-2511	145	147	usually	usually	ADV
american_scientific_journal-2511	145	148	contain	contain	VERB
american_scientific_journal-2511	145	149	a	a	DET
american_scientific_journal-2511	145	150	region	region	NOUN
american_scientific_journal-2511	145	151	of	of	ADP
american_scientific_journal-2511	145	152	dna	dna	PROPN
american_scientific_journal-2511	145	153	,	,	PUNCT
american_scientific_journal-2511	145	154	referred	refer	VERB
american_scientific_journal-2511	145	155	to	to	ADP
american_scientific_journal-2511	145	156	as	as	ADP
american_scientific_journal-2511	145	157	a	a	DET
american_scientific_journal-2511	145	158	transcriptional	transcriptional	ADJ
american_scientific_journal-2511	145	159	terminator	terminator	NOUN
american_scientific_journal-2511	145	160	downstream	downstream	ADV
american_scientific_journal-2511	145	161	from	from	ADP
american_scientific_journal-2511	145	162	the	the	DET
american_scientific_journal-2511	145	163	multiple	multiple	ADJ
american_scientific_journal-2511	145	164	cloning	cloning	NOUN
american_scientific_journal-2511	145	165	site	site	NOUN
american_scientific_journal-2511	145	166	where	where	SCONJ
american_scientific_journal-2511	145	167	the	the	DET
american_scientific_journal-2511	145	168	cloned	cloned	ADJ
american_scientific_journal-2511	145	169	gene	gene	NOUN
american_scientific_journal-2511	145	170	will	will	AUX
american_scientific_journal-2511	145	171	be	be	AUX
american_scientific_journal-2511	145	172	inserted	insert	VERB
american_scientific_journal-2511	145	173	.	.	PUNCT
american_scientific_journal-2511	146	1	rna	rna	NOUN
american_scientific_journal-2511	146	2	polymerase	polymerase	NOUN
american_scientific_journal-2511	146	3	reaching	reach	VERB
american_scientific_journal-2511	146	4	this	this	DET
american_scientific_journal-2511	146	5	site	site	NOUN
american_scientific_journal-2511	146	6	ceases	cease	VERB
american_scientific_journal-2511	146	7	transcription	transcription	NOUN
american_scientific_journal-2511	146	8	,	,	PUNCT
american_scientific_journal-2511	146	9	so	so	CCONJ
american_scientific_journal-2511	146	10	only	only	ADV
american_scientific_journal-2511	146	11	the	the	DET
american_scientific_journal-2511	146	12	cloned	cloned	ADJ
american_scientific_journal-2511	146	13	gene	gene	NOUN
american_scientific_journal-2511	146	14	itself	itself	PRON
american_scientific_journal-2511	146	15	will	will	AUX
american_scientific_journal-2511	146	16	be	be	AUX
american_scientific_journal-2511	146	17	transcribed	transcribe	VERB
american_scientific_journal-2511	146	18	from	from	ADP
american_scientific_journal-2511	146	19	the	the	DET
american_scientific_journal-2511	146	20	strong	strong	ADJ
american_scientific_journal-2511	146	21	promoter	promoter	NOUN
american_scientific_journal-2511	147	1	[	[	X
american_scientific_journal-2511	147	2	20,9	20,9	NUM
american_scientific_journal-2511	147	3	]	]	PUNCT
american_scientific_journal-2511	147	4	.	.	PUNCT
american_scientific_journal-2511	148	1	in	in	ADP
american_scientific_journal-2511	148	2	this	this	DET
american_scientific_journal-2511	148	3	study	study	NOUN
american_scientific_journal-2511	148	4	,	,	PUNCT
american_scientific_journal-2511	148	5	constructive	constructive	ADJ
american_scientific_journal-2511	148	6	vector	vector	NOUN
american_scientific_journal-2511	148	7	pet16b	pet16b	NOUN
american_scientific_journal-2511	148	8	-	-	PUNCT
american_scientific_journal-2511	148	9	hr	hr	NOUN
american_scientific_journal-2511	148	10	was	be	AUX
american_scientific_journal-2511	148	11	extracted	extract	VERB
american_scientific_journal-2511	148	12	figure	figure	NOUN
american_scientific_journal-2511	148	13	7	7	NUM
american_scientific_journal-2511	148	14	and	and	CCONJ
american_scientific_journal-2511	148	15	by	by	ADP
american_scientific_journal-2511	148	16	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	148	17	with	with	ADP
american_scientific_journal-2511	148	18	control	control	NOUN
american_scientific_journal-2511	148	19	the	the	DET
american_scientific_journal-2511	148	20	resulted	resulted	ADJ
american_scientific_journal-2511	148	21	band	band	NOUN
american_scientific_journal-2511	148	22	of	of	ADP
american_scientific_journal-2511	148	23	constructive	constructive	ADJ
american_scientific_journal-2511	148	24	vector	vector	NOUN
american_scientific_journal-2511	148	25	about	about	ADP
american_scientific_journal-2511	148	26	5966bp	5966bp	ADJ
american_scientific_journal-2511	148	27	in	in	ADP
american_scientific_journal-2511	148	28	comparison	comparison	NOUN
american_scientific_journal-2511	148	29	with	with	ADP
american_scientific_journal-2511	148	30	control	control	NOUN
american_scientific_journal-2511	148	31	about	about	ADP
american_scientific_journal-2511	148	32	5711bp	5711bp	NOUN
american_scientific_journal-2511	148	33	that	that	PRON
american_scientific_journal-2511	148	34	’s	’	VERB
american_scientific_journal-2511	148	35	mean	mean	ADJ
american_scientific_journal-2511	148	36	presence	presence	NOUN
american_scientific_journal-2511	148	37	of	of	ADP
american_scientific_journal-2511	148	38	target	target	NOUN
american_scientific_journal-2511	148	39	gene	gene	NOUN
american_scientific_journal-2511	148	40	in	in	ADP
american_scientific_journal-2511	148	41	constructive	constructive	ADJ
american_scientific_journal-2511	148	42	vector	vector	NOUN
american_scientific_journal-2511	148	43	,	,	PUNCT
american_scientific_journal-2511	148	44	for	for	ADP
american_scientific_journal-2511	148	45	more	more	ADJ
american_scientific_journal-2511	148	46	detection	detection	NOUN
american_scientific_journal-2511	148	47	from	from	ADP
american_scientific_journal-2511	148	48	the	the	DET
american_scientific_journal-2511	148	49	constructive	constructive	ADJ
american_scientific_journal-2511	148	50	vector	vector	NOUN
american_scientific_journal-2511	148	51	which	which	PRON
american_scientific_journal-2511	148	52	extracted	extract	VERB
american_scientific_journal-2511	148	53	from	from	ADP
american_scientific_journal-2511	148	54	transformed	transform	VERB
american_scientific_journal-2511	148	55	cell	cell	NOUN
american_scientific_journal-2511	148	56	,	,	PUNCT
american_scientific_journal-2511	148	57	hirudin	hirudin	VERB
american_scientific_journal-2511	148	58	genes	gene	NOUN
american_scientific_journal-2511	148	59	was	be	AUX
american_scientific_journal-2511	148	60	amplifying	amplify	VERB
american_scientific_journal-2511	148	61	with	with	ADP
american_scientific_journal-2511	148	62	pcr	pcr	ADJ
american_scientific_journal-2511	148	63	cycle	cycle	NOUN
american_scientific_journal-2511	148	64	,	,	PUNCT
american_scientific_journal-2511	148	65	the	the	DET
american_scientific_journal-2511	148	66	resulted	resulted	ADJ
american_scientific_journal-2511	148	67	band	band	NOUN
american_scientific_journal-2511	148	68	from	from	ADP
american_scientific_journal-2511	148	69	gel	gel	NOUN
american_scientific_journal-2511	148	70	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	148	71	about	about	ADP
american_scientific_journal-2511	148	72	255bp	255bp	ADJ
american_scientific_journal-2511	148	73	figure	figure	NOUN
american_scientific_journal-2511	148	74	8	8	NUM
american_scientific_journal-2511	148	75	.	.	PUNCT
american_scientific_journal-2511	149	1	the	the	DET
american_scientific_journal-2511	149	2	classic	classic	ADJ
american_scientific_journal-2511	149	3	method	method	NOUN
american_scientific_journal-2511	149	4	for	for	ADP
american_scientific_journal-2511	149	5	screening	screen	VERB
american_scientific_journal-2511	149	6	involves	involve	VERB
american_scientific_journal-2511	149	7	extraction	extraction	NOUN
american_scientific_journal-2511	149	8	of	of	ADP
american_scientific_journal-2511	149	9	constructive	constructive	ADJ
american_scientific_journal-2511	149	10	plasmid	plasmid	NOUN
american_scientific_journal-2511	149	11	from	from	ADP
american_scientific_journal-2511	149	12	transformed	transform	VERB
american_scientific_journal-2511	149	13	bacteria	bacteria	NOUN
american_scientific_journal-2511	149	14	followed	follow	VERB
american_scientific_journal-2511	149	15	by	by	ADP
american_scientific_journal-2511	149	16	restriction	restriction	NOUN
american_scientific_journal-2511	149	17	digestion	digestion	NOUN
american_scientific_journal-2511	149	18	,	,	PUNCT
american_scientific_journal-2511	149	19	after	after	ADP
american_scientific_journal-2511	149	20	that	that	PRON
american_scientific_journal-2511	149	21	a	a	DET
american_scientific_journal-2511	149	22	restriction	restriction	NOUN
american_scientific_journal-2511	149	23	enzyme	enzyme	NOUN
american_scientific_journal-2511	149	24	was	be	AUX
american_scientific_journal-2511	149	25	chosen	choose	VERB
american_scientific_journal-2511	149	26	,	,	PUNCT
american_scientific_journal-2511	149	27	in	in	ADP
american_scientific_journal-2511	149	28	this	this	DET
american_scientific_journal-2511	149	29	research	research	NOUN
american_scientific_journal-2511	149	30	hidiii	hidiii	NOUN
american_scientific_journal-2511	149	31	and	and	CCONJ
american_scientific_journal-2511	149	32	ndei	ndei	NOUN
american_scientific_journal-2511	149	33	choose	choose	VERB
american_scientific_journal-2511	149	34	to	to	PART
american_scientific_journal-2511	149	35	eliminate	eliminate	VERB
american_scientific_journal-2511	149	36	a	a	DET
american_scientific_journal-2511	149	37	fragment	fragment	NOUN
american_scientific_journal-2511	149	38	from	from	ADP
american_scientific_journal-2511	149	39	constructive	constructive	ADJ
american_scientific_journal-2511	149	40	plasmid	plasmid	NOUN
american_scientific_journal-2511	149	41	which	which	PRON
american_scientific_journal-2511	149	42	contain	contain	VERB
american_scientific_journal-2511	149	43	our	our	PRON
american_scientific_journal-2511	149	44	insert	insert	NOUN
american_scientific_journal-2511	149	45	after	after	ADP
american_scientific_journal-2511	149	46	amplification	amplification	NOUN
american_scientific_journal-2511	149	47	with	with	ADP
american_scientific_journal-2511	149	48	hr	hr	NOUN
american_scientific_journal-2511	149	49	gene	gene	NOUN
american_scientific_journal-2511	149	50	primer	primer	NOUN
american_scientific_journal-2511	149	51	it	it	PRON
american_scientific_journal-2511	149	52	's	be	AUX
american_scientific_journal-2511	149	53	easy	easy	ADJ
american_scientific_journal-2511	149	54	to	to	PART
american_scientific_journal-2511	149	55	detect	detect	VERB
american_scientific_journal-2511	149	56	if	if	SCONJ
american_scientific_journal-2511	149	57	our	our	PRON
american_scientific_journal-2511	149	58	plasmid	plasmid	NOUN
american_scientific_journal-2511	149	59	contains	contain	VERB
american_scientific_journal-2511	149	60	insert	insert	NOUN
american_scientific_journal-2511	149	61	or	or	CCONJ
american_scientific_journal-2511	149	62	not	not	PART
american_scientific_journal-2511	149	63	this	this	DET
american_scientific_journal-2511	149	64	process	process	NOUN
american_scientific_journal-2511	149	65	was	be	AUX
american_scientific_journal-2511	149	66	done	do	VERB
american_scientific_journal-2511	149	67	with	with	ADP
american_scientific_journal-2511	149	68	cutting	cut	VERB
american_scientific_journal-2511	149	69	for	for	ADP
american_scientific_journal-2511	149	70	control	control	NOUN
american_scientific_journal-2511	149	71	(	(	PUNCT
american_scientific_journal-2511	149	72	plasmid	plasmid	ADJ
american_scientific_journal-2511	149	73	without	without	ADP
american_scientific_journal-2511	149	74	insert	insert	NOUN
american_scientific_journal-2511	149	75	)	)	PUNCT
american_scientific_journal-2511	149	76	for	for	ADP
american_scientific_journal-2511	149	77	comparison	comparison	NOUN
american_scientific_journal-2511	149	78	figure	figure	NOUN
american_scientific_journal-2511	149	79	9	9	NUM
american_scientific_journal-2511	149	80	[	[	X
american_scientific_journal-2511	149	81	21	21	NUM
american_scientific_journal-2511	149	82	]	]	PUNCT
american_scientific_journal-2511	149	83	.	.	PUNCT
american_scientific_journal-2511	150	1	to	to	PART
american_scientific_journal-2511	150	2	detect	detect	VERB
american_scientific_journal-2511	150	3	gene	gene	NOUN
american_scientific_journal-2511	150	4	expression	expression	NOUN
american_scientific_journal-2511	150	5	for	for	ADP
american_scientific_journal-2511	150	6	hirudin	hirudin	VERB
american_scientific_journal-2511	150	7	gene	gene	NOUN
american_scientific_journal-2511	150	8	,	,	PUNCT
american_scientific_journal-2511	150	9	real	real	ADJ
american_scientific_journal-2511	150	10	time	time	NOUN
american_scientific_journal-2511	150	11	pcr	pcr	PROPN
american_scientific_journal-2511	150	12	has	have	AUX
american_scientific_journal-2511	150	13	been	be	AUX
american_scientific_journal-2511	150	14	done	do	VERB
american_scientific_journal-2511	150	15	as	as	ADP
american_scientific_journal-2511	150	16	the	the	DET
american_scientific_journal-2511	150	17	results	result	NOUN
american_scientific_journal-2511	150	18	shown	show	VERB
american_scientific_journal-2511	150	19	in	in	ADP
american_scientific_journal-2511	150	20	figures	figure	NOUN
american_scientific_journal-2511	150	21	10&11	10&11	NUM
american_scientific_journal-2511	150	22	,	,	PUNCT
american_scientific_journal-2511	150	23	threshold	threshold	NOUN
american_scientific_journal-2511	150	24	curve	curve	NOUN
american_scientific_journal-2511	150	25	illustrates	illustrate	VERB
american_scientific_journal-2511	150	26	a	a	DET
american_scientific_journal-2511	150	27	function	function	NOUN
american_scientific_journal-2511	150	28	of	of	ADP
american_scientific_journal-2511	150	29	the	the	DET
american_scientific_journal-2511	150	30	amount	amount	NOUN
american_scientific_journal-2511	150	31	of	of	ADP
american_scientific_journal-2511	150	32	background	background	NOUN
american_scientific_journal-2511	150	33	fluorescence	fluorescence	NOUN
american_scientific_journal-2511	150	34	and	and	CCONJ
american_scientific_journal-2511	150	35	is	be	AUX
american_scientific_journal-2511	150	36	plotted	plot	VERB
american_scientific_journal-2511	150	37	at	at	ADP
american_scientific_journal-2511	150	38	a	a	DET
american_scientific_journal-2511	150	39	point	point	NOUN
american_scientific_journal-2511	150	40	in	in	ADP
american_scientific_journal-2511	150	41	which	which	PRON
american_scientific_journal-2511	150	42	the	the	DET
american_scientific_journal-2511	150	43	signal	signal	NOUN
american_scientific_journal-2511	150	44	generated	generate	VERB
american_scientific_journal-2511	150	45	from	from	ADP
american_scientific_journal-2511	150	46	a	a	DET
american_scientific_journal-2511	150	47	sample	sample	NOUN
american_scientific_journal-2511	150	48	is	be	AUX
american_scientific_journal-2511	150	49	significantly	significantly	ADV
american_scientific_journal-2511	150	50	greater	great	ADJ
american_scientific_journal-2511	150	51	than	than	ADP
american_scientific_journal-2511	150	52	background	background	NOUN
american_scientific_journal-2511	150	53	fluorescence	fluorescence	NOUN
american_scientific_journal-2511	150	54	.	.	PUNCT
american_scientific_journal-2511	151	1	therefore	therefore	ADV
american_scientific_journal-2511	151	2	,	,	PUNCT
american_scientific_journal-2511	151	3	the	the	DET
american_scientific_journal-2511	151	4	fractional	fractional	ADJ
american_scientific_journal-2511	151	5	number	number	NOUN
american_scientific_journal-2511	151	6	of	of	ADP
american_scientific_journal-2511	151	7	pcr	pcr	ADJ
american_scientific_journal-2511	151	8	cycles	cycle	NOUN
american_scientific_journal-2511	151	9	required	require	VERB
american_scientific_journal-2511	151	10	to	to	PART
american_scientific_journal-2511	151	11	generate	generate	VERB
american_scientific_journal-2511	151	12	enough	enough	ADJ
american_scientific_journal-2511	151	13	fluorescent	fluorescent	ADJ
american_scientific_journal-2511	151	14	signal	signal	NOUN
american_scientific_journal-2511	151	15	to	to	PART
american_scientific_journal-2511	151	16	reach	reach	VERB
american_scientific_journal-2511	151	17	this	this	DET
american_scientific_journal-2511	151	18	threshold	threshold	NOUN
american_scientific_journal-2511	151	19	is	be	AUX
american_scientific_journal-2511	151	20	defined	define	VERB
american_scientific_journal-2511	151	21	as	as	ADP
american_scientific_journal-2511	151	22	the	the	DET
american_scientific_journal-2511	151	23	ct	ct	PROPN
american_scientific_journal-2511	151	24	value	value	NOUN
american_scientific_journal-2511	151	25	.	.	PUNCT
american_scientific_journal-2511	152	1	these	these	DET
american_scientific_journal-2511	152	2	ct	ct	PROPN
american_scientific_journal-2511	152	3	values	value	NOUN
american_scientific_journal-2511	152	4	are	be	AUX
american_scientific_journal-2511	152	5	directly	directly	ADV
american_scientific_journal-2511	152	6	proportionate	proportionate	ADJ
american_scientific_journal-2511	152	7	to	to	ADP
american_scientific_journal-2511	152	8	the	the	DET
american_scientific_journal-2511	152	9	amount	amount	NOUN
american_scientific_journal-2511	152	10	of	of	ADP
american_scientific_journal-2511	152	11	starting	start	VERB
american_scientific_journal-2511	152	12	template	template	NOUN
american_scientific_journal-2511	152	13	and	and	CCONJ
american_scientific_journal-2511	152	14	are	be	AUX
american_scientific_journal-2511	152	15	the	the	DET
american_scientific_journal-2511	152	16	american	american	ADJ
american_scientific_journal-2511	152	17	scientific	scientific	ADJ
american_scientific_journal-2511	152	18	research	research	PROPN
american_scientific_journal-2511	152	19	journal	journal	NOUN
american_scientific_journal-2511	152	20	for	for	ADP
american_scientific_journal-2511	152	21	engineering	engineering	NOUN
american_scientific_journal-2511	152	22	,	,	PUNCT
american_scientific_journal-2511	152	23	technology	technology	NOUN
american_scientific_journal-2511	152	24	,	,	PUNCT
american_scientific_journal-2511	152	25	and	and	CCONJ
american_scientific_journal-2511	152	26	sciences	science	NOUN
american_scientific_journal-2511	152	27	(	(	PUNCT
american_scientific_journal-2511	152	28	asrjets	asrjet	NOUN
american_scientific_journal-2511	152	29	)	)	PUNCT
american_scientific_journal-2511	152	30	(	(	PUNCT
american_scientific_journal-2511	152	31	2017	2017	NUM
american_scientific_journal-2511	152	32	)	)	PUNCT
american_scientific_journal-2511	152	33	volume	volume	NOUN
american_scientific_journal-2511	152	34	27	27	NUM
american_scientific_journal-2511	152	35	,	,	PUNCT
american_scientific_journal-2511	152	36	no	no	DET
american_scientific_journal-2511	152	37	1	1	NUM
american_scientific_journal-2511	152	38	,	,	PUNCT
american_scientific_journal-2511	152	39	pp	pp	ADV
american_scientific_journal-2511	152	40	25	25	NUM
american_scientific_journal-2511	152	41	-	-	SYM
american_scientific_journal-2511	152	42	42	42	NUM
american_scientific_journal-2511	152	43	39	39	NUM
american_scientific_journal-2511	152	44	basis	basis	NOUN
american_scientific_journal-2511	152	45	for	for	ADP
american_scientific_journal-2511	152	46	calculating	calculate	VERB
american_scientific_journal-2511	152	47	mrna	mrna	PROPN
american_scientific_journal-2511	152	48	expression	expression	NOUN
american_scientific_journal-2511	152	49	levels	level	NOUN
american_scientific_journal-2511	152	50	or	or	CCONJ
american_scientific_journal-2511	152	51	dna	dna	PROPN
american_scientific_journal-2511	152	52	copy	copy	VERB
american_scientific_journal-2511	152	53	number	number	NOUN
american_scientific_journal-2511	152	54	measurement	measurement	NOUN
american_scientific_journal-2511	153	1	[	[	X
american_scientific_journal-2511	153	2	22,23	22,23	NOUN
american_scientific_journal-2511	153	3	]	]	PUNCT
american_scientific_journal-2511	153	4	.	.	PUNCT
american_scientific_journal-2511	154	1	in	in	ADP
american_scientific_journal-2511	154	2	spite	spite	NOUN
american_scientific_journal-2511	154	3	of	of	ADP
american_scientific_journal-2511	154	4	the	the	DET
american_scientific_journal-2511	154	5	importance	importance	NOUN
american_scientific_journal-2511	154	6	of	of	ADP
american_scientific_journal-2511	154	7	ct	ct	NUM
american_scientific_journal-2511	154	8	curve	curve	NOUN
american_scientific_journal-2511	154	9	but	but	CCONJ
american_scientific_journal-2511	154	10	it	it	PRON
american_scientific_journal-2511	154	11	depends	depend	VERB
american_scientific_journal-2511	154	12	on	on	ADP
american_scientific_journal-2511	154	13	fluorescent	fluorescent	ADJ
american_scientific_journal-2511	154	14	signal	signal	NOUN
american_scientific_journal-2511	154	15	emitting	emit	VERB
american_scientific_journal-2511	154	16	from	from	ADP
american_scientific_journal-2511	154	17	binding	bind	VERB
american_scientific_journal-2511	154	18	syber	syber	PROPN
american_scientific_journal-2511	154	19	green	green	PROPN
american_scientific_journal-2511	154	20	dye	dye	NOUN
american_scientific_journal-2511	154	21	to	to	ADP
american_scientific_journal-2511	154	22	the	the	DET
american_scientific_journal-2511	154	23	dsdna	dsdna	NOUN
american_scientific_journal-2511	154	24	this	this	DET
american_scientific_journal-2511	154	25	simplicity	simplicity	NOUN
american_scientific_journal-2511	154	26	means	mean	VERB
american_scientific_journal-2511	154	27	that	that	SCONJ
american_scientific_journal-2511	154	28	they	they	PRON
american_scientific_journal-2511	154	29	do	do	AUX
american_scientific_journal-2511	154	30	not	not	PART
american_scientific_journal-2511	154	31	distinguish	distinguish	VERB
american_scientific_journal-2511	154	32	between	between	ADP
american_scientific_journal-2511	154	33	different	different	ADJ
american_scientific_journal-2511	154	34	dsdna	dsdna	NOUN
american_scientific_journal-2511	154	35	products	product	NOUN
american_scientific_journal-2511	154	36	(	(	PUNCT
american_scientific_journal-2511	154	37	example	example	NOUN
american_scientific_journal-2511	154	38	primer	primer	NOUN
american_scientific_journal-2511	154	39	dimer	dimer	NOUN
american_scientific_journal-2511	154	40	)	)	PUNCT
american_scientific_journal-2511	154	41	and	and	CCONJ
american_scientific_journal-2511	154	42	it	it	PRON
american_scientific_journal-2511	154	43	is	be	AUX
american_scientific_journal-2511	154	44	important	important	ADJ
american_scientific_journal-2511	154	45	that	that	SCONJ
american_scientific_journal-2511	154	46	pcr	pcr	ADJ
american_scientific_journal-2511	154	47	reactions	reaction	NOUN
american_scientific_journal-2511	154	48	be	be	AUX
american_scientific_journal-2511	154	49	optimized	optimize	VERB
american_scientific_journal-2511	154	50	for	for	SCONJ
american_scientific_journal-2511	154	51	only	only	ADV
american_scientific_journal-2511	154	52	the	the	DET
american_scientific_journal-2511	154	53	target	target	NOUN
american_scientific_journal-2511	154	54	amplicon	amplicon	NOUN
american_scientific_journal-2511	154	55	is	be	AUX
american_scientific_journal-2511	154	56	present	present	ADJ
american_scientific_journal-2511	154	57	,	,	PUNCT
american_scientific_journal-2511	154	58	so	so	CCONJ
american_scientific_journal-2511	154	59	other	other	ADJ
american_scientific_journal-2511	154	60	method	method	NOUN
american_scientific_journal-2511	154	61	employed	employ	VERB
american_scientific_journal-2511	154	62	to	to	PART
american_scientific_journal-2511	154	63	distinguish	distinguish	VERB
american_scientific_journal-2511	154	64	between	between	ADP
american_scientific_journal-2511	154	65	different	different	ADJ
american_scientific_journal-2511	154	66	products	product	NOUN
american_scientific_journal-2511	154	67	which	which	PRON
american_scientific_journal-2511	154	68	is	be	AUX
american_scientific_journal-2511	154	69	(	(	PUNCT
american_scientific_journal-2511	154	70	melting	melt	VERB
american_scientific_journal-2511	154	71	point	point	NOUN
american_scientific_journal-2511	154	72	analysis	analysis	NOUN
american_scientific_journal-2511	154	73	)	)	PUNCT
american_scientific_journal-2511	154	74	.	.	PUNCT
american_scientific_journal-2511	155	1	melting	melting	NOUN
american_scientific_journal-2511	155	2	point	point	NOUN
american_scientific_journal-2511	155	3	analysis	analysis	NOUN
american_scientific_journal-2511	155	4	is	be	AUX
american_scientific_journal-2511	155	5	used	use	VERB
american_scientific_journal-2511	155	6	to	to	PART
american_scientific_journal-2511	155	7	distinguish	distinguish	VERB
american_scientific_journal-2511	155	8	target	target	NOUN
american_scientific_journal-2511	155	9	amplicons	amplicon	NOUN
american_scientific_journal-2511	155	10	from	from	ADP
american_scientific_journal-2511	155	11	pcr	pcr	ADJ
american_scientific_journal-2511	155	12	artifacts	artifact	NOUN
american_scientific_journal-2511	155	13	such	such	ADJ
american_scientific_journal-2511	155	14	as	as	ADP
american_scientific_journal-2511	155	15	primer	primer	NOUN
american_scientific_journal-2511	155	16	-	-	PUNCT
american_scientific_journal-2511	155	17	dimer	dimer	NOUN
american_scientific_journal-2511	155	18	,	,	PUNCT
american_scientific_journal-2511	155	19	the	the	DET
american_scientific_journal-2511	155	20	utility	utility	NOUN
american_scientific_journal-2511	155	21	of	of	ADP
american_scientific_journal-2511	155	22	melting	melting	NOUN
american_scientific_journal-2511	155	23	point	point	NOUN
american_scientific_journal-2511	155	24	analysis	analysis	NOUN
american_scientific_journal-2511	155	25	derives	derive	VERB
american_scientific_journal-2511	155	26	from	from	ADP
american_scientific_journal-2511	155	27	the	the	DET
american_scientific_journal-2511	155	28	observation	observation	NOUN
american_scientific_journal-2511	155	29	that	that	SCONJ
american_scientific_journal-2511	155	30	the	the	DET
american_scientific_journal-2511	155	31	temperature	temperature	NOUN
american_scientific_journal-2511	155	32	at	at	ADP
american_scientific_journal-2511	155	33	which	which	PRON
american_scientific_journal-2511	155	34	a	a	DET
american_scientific_journal-2511	155	35	dna	dna	NOUN
american_scientific_journal-2511	155	36	duplex	duplex	NOUN
american_scientific_journal-2511	155	37	will	will	AUX
american_scientific_journal-2511	155	38	denature	denature	VERB
american_scientific_journal-2511	155	39	is	be	AUX
american_scientific_journal-2511	155	40	dependent	dependent	ADJ
american_scientific_journal-2511	155	41	upon	upon	SCONJ
american_scientific_journal-2511	155	42	length	length	NOUN
american_scientific_journal-2511	155	43	and	and	CCONJ
american_scientific_journal-2511	155	44	nucleotide	nucleotide	ADJ
american_scientific_journal-2511	155	45	composition	composition	NOUN
american_scientific_journal-2511	155	46	.	.	PUNCT
american_scientific_journal-2511	156	1	fluorescence	fluorescence	ADJ
american_scientific_journal-2511	156	2	measurements	measurement	NOUN
american_scientific_journal-2511	156	3	are	be	AUX
american_scientific_journal-2511	156	4	made	make	VERB
american_scientific_journal-2511	156	5	while	while	SCONJ
american_scientific_journal-2511	156	6	slowly	slowly	ADV
american_scientific_journal-2511	156	7	increasing	increase	VERB
american_scientific_journal-2511	156	8	the	the	DET
american_scientific_journal-2511	156	9	temperature	temperature	NOUN
american_scientific_journal-2511	156	10	of	of	ADP
american_scientific_journal-2511	156	11	the	the	DET
american_scientific_journal-2511	156	12	reaction	reaction	NOUN
american_scientific_journal-2511	156	13	products	product	NOUN
american_scientific_journal-2511	156	14	(	(	PUNCT
american_scientific_journal-2511	156	15	for	for	ADP
american_scientific_journal-2511	156	16	example	example	NOUN
american_scientific_journal-2511	156	17	,	,	PUNCT
american_scientific_journal-2511	156	18	from	from	ADP
american_scientific_journal-2511	156	19	60	60	NUM
american_scientific_journal-2511	156	20	◦	◦	NOUN
american_scientific_journal-2511	156	21	to	to	ADP
american_scientific_journal-2511	156	22	95	95	NUM
american_scientific_journal-2511	156	23	◦	◦	NOUN
american_scientific_journal-2511	156	24	c	c	NOUN
american_scientific_journal-2511	156	25	)	)	PUNCT
american_scientific_journal-2511	156	26	.	.	PUNCT
american_scientific_journal-2511	157	1	at	at	ADP
american_scientific_journal-2511	157	2	the	the	DET
american_scientific_journal-2511	157	3	low	low	ADJ
american_scientific_journal-2511	157	4	temperature	temperature	NOUN
american_scientific_journal-2511	157	5	,	,	PUNCT
american_scientific_journal-2511	157	6	the	the	DET
american_scientific_journal-2511	157	7	amplicons	amplicon	NOUN
american_scientific_journal-2511	157	8	are	be	AUX
american_scientific_journal-2511	157	9	all	all	ADV
american_scientific_journal-2511	157	10	double	double	ADV
american_scientific_journal-2511	157	11	stranded	strand	VERB
american_scientific_journal-2511	157	12	and	and	CCONJ
american_scientific_journal-2511	157	13	thus	thus	ADV
american_scientific_journal-2511	157	14	bind	bind	VERB
american_scientific_journal-2511	157	15	the	the	DET
american_scientific_journal-2511	157	16	sybr	sybr	ADJ
american_scientific_journal-2511	157	17	green	green	PROPN
american_scientific_journal-2511	157	18	dye	dye	PROPN
american_scientific_journal-2511	157	19	,	,	PUNCT
american_scientific_journal-2511	157	20	producing	produce	VERB
american_scientific_journal-2511	157	21	a	a	DET
american_scientific_journal-2511	157	22	strong	strong	ADJ
american_scientific_journal-2511	157	23	fluorescence	fluorescence	NOUN
american_scientific_journal-2511	157	24	signal	signal	NOUN
american_scientific_journal-2511	157	25	.	.	PUNCT
american_scientific_journal-2511	158	1	as	as	ADP
american_scientific_journal-2511	158	2	the	the	DET
american_scientific_journal-2511	158	3	temperature	temperature	NOUN
american_scientific_journal-2511	158	4	increases	increase	NOUN
american_scientific_journal-2511	158	5	,	,	PUNCT
american_scientific_journal-2511	158	6	the	the	DET
american_scientific_journal-2511	158	7	pcr	pcr	NOUN
american_scientific_journal-2511	158	8	products	product	NOUN
american_scientific_journal-2511	158	9	are	be	AUX
american_scientific_journal-2511	158	10	denatured	denature	VERB
american_scientific_journal-2511	158	11	,	,	PUNCT
american_scientific_journal-2511	158	12	resulting	result	VERB
american_scientific_journal-2511	158	13	in	in	ADP
american_scientific_journal-2511	158	14	a	a	DET
american_scientific_journal-2511	158	15	decrease	decrease	NOUN
american_scientific_journal-2511	158	16	in	in	ADP
american_scientific_journal-2511	158	17	fluorescence	fluorescence	NOUN
american_scientific_journal-2511	158	18	[	[	X
american_scientific_journal-2511	158	19	23	23	NUM
american_scientific_journal-2511	158	20	]	]	PUNCT
american_scientific_journal-2511	158	21	.	.	PUNCT
american_scientific_journal-2511	159	1	a	a	DET
american_scientific_journal-2511	159	2	melting	melt	VERB
american_scientific_journal-2511	159	3	curve	curve	NOUN
american_scientific_journal-2511	159	4	charts	chart	NOUN
american_scientific_journal-2511	159	5	the	the	DET
american_scientific_journal-2511	159	6	change	change	NOUN
american_scientific_journal-2511	159	7	in	in	ADP
american_scientific_journal-2511	159	8	fluorescence	fluorescence	NOUN
american_scientific_journal-2511	159	9	observed	observe	VERB
american_scientific_journal-2511	159	10	when	when	SCONJ
american_scientific_journal-2511	159	11	double	double	ADJ
american_scientific_journal-2511	159	12	-	-	PUNCT
american_scientific_journal-2511	159	13	stranded	strand	VERB
american_scientific_journal-2511	159	14	dna	dna	NOUN
american_scientific_journal-2511	159	15	(	(	PUNCT
american_scientific_journal-2511	159	16	dsdna	dsdna	NOUN
american_scientific_journal-2511	159	17	)	)	PUNCT
american_scientific_journal-2511	159	18	with	with	ADP
american_scientific_journal-2511	159	19	incorporated	incorporate	VERB
american_scientific_journal-2511	159	20	dye	dye	NOUN
american_scientific_journal-2511	159	21	molecules	molecule	NOUN
american_scientific_journal-2511	159	22	dissociates	dissociate	NOUN
american_scientific_journal-2511	159	23	,	,	PUNCT
american_scientific_journal-2511	159	24	or	or	CCONJ
american_scientific_journal-2511	159	25	“	"	PUNCT
american_scientific_journal-2511	159	26	melts	melt	NOUN
american_scientific_journal-2511	159	27	”	"	PUNCT
american_scientific_journal-2511	159	28	into	into	ADP
american_scientific_journal-2511	159	29	single	single	ADJ
american_scientific_journal-2511	159	30	-	-	PUNCT
american_scientific_journal-2511	159	31	stranded	strand	VERB
american_scientific_journal-2511	159	32	dna	dna	NOUN
american_scientific_journal-2511	159	33	(	(	PUNCT
american_scientific_journal-2511	159	34	ssdna	ssdna	ADV
american_scientific_journal-2511	159	35	)	)	PUNCT
american_scientific_journal-2511	159	36	as	as	SCONJ
american_scientific_journal-2511	159	37	the	the	DET
american_scientific_journal-2511	159	38	temperature	temperature	NOUN
american_scientific_journal-2511	159	39	of	of	ADP
american_scientific_journal-2511	159	40	the	the	DET
american_scientific_journal-2511	159	41	reaction	reaction	NOUN
american_scientific_journal-2511	159	42	is	be	AUX
american_scientific_journal-2511	159	43	raised	raise	VERB
american_scientific_journal-2511	159	44	.	.	PUNCT
american_scientific_journal-2511	160	1	when	when	SCONJ
american_scientific_journal-2511	160	2	double	double	ADJ
american_scientific_journal-2511	160	3	-	-	PUNCT
american_scientific_journal-2511	160	4	stranded	strand	VERB
american_scientific_journal-2511	160	5	dna	dna	NOUN
american_scientific_journal-2511	160	6	bound	bind	VERB
american_scientific_journal-2511	160	7	with	with	ADP
american_scientific_journal-2511	160	8	sybr	sybr	PROPN
american_scientific_journal-2511	160	9	green	green	PROPN
american_scientific_journal-2511	160	10	is	be	AUX
american_scientific_journal-2511	160	11	heated	heat	VERB
american_scientific_journal-2511	160	12	a	a	DET
american_scientific_journal-2511	160	13	sudden	sudden	ADJ
american_scientific_journal-2511	160	14	decrease	decrease	NOUN
american_scientific_journal-2511	160	15	in	in	ADP
american_scientific_journal-2511	160	16	fluorescence	fluorescence	NOUN
american_scientific_journal-2511	160	17	is	be	AUX
american_scientific_journal-2511	160	18	detected	detect	VERB
american_scientific_journal-2511	160	19	when	when	SCONJ
american_scientific_journal-2511	160	20	the	the	DET
american_scientific_journal-2511	160	21	melting	melting	NOUN
american_scientific_journal-2511	160	22	point	point	NOUN
american_scientific_journal-2511	160	23	(	(	PUNCT
american_scientific_journal-2511	160	24	tm	tm	NOUN
american_scientific_journal-2511	160	25	)	)	PUNCT
american_scientific_journal-2511	160	26	is	be	AUX
american_scientific_journal-2511	160	27	reached	reach	VERB
american_scientific_journal-2511	160	28	,	,	PUNCT
american_scientific_journal-2511	160	29	due	due	ADP
american_scientific_journal-2511	160	30	to	to	ADP
american_scientific_journal-2511	160	31	dissociation	dissociation	NOUN
american_scientific_journal-2511	160	32	of	of	ADP
american_scientific_journal-2511	160	33	the	the	DET
american_scientific_journal-2511	160	34	dna	dna	PROPN
american_scientific_journal-2511	160	35	strands	strand	NOUN
american_scientific_journal-2511	160	36	and	and	CCONJ
american_scientific_journal-2511	160	37	subsequent	subsequent	ADJ
american_scientific_journal-2511	160	38	release	release	NOUN
american_scientific_journal-2511	160	39	of	of	ADP
american_scientific_journal-2511	160	40	the	the	DET
american_scientific_journal-2511	160	41	dye	dye	NOUN
american_scientific_journal-2511	160	42	,	,	PUNCT
american_scientific_journal-2511	160	43	so	so	ADV
american_scientific_journal-2511	160	44	the	the	DET
american_scientific_journal-2511	160	45	fluorescence	fluorescence	NOUN
american_scientific_journal-2511	160	46	is	be	AUX
american_scientific_journal-2511	160	47	plotted	plot	VERB
american_scientific_journal-2511	160	48	against	against	ADP
american_scientific_journal-2511	160	49	temperature	temperature	NOUN
american_scientific_journal-2511	160	50	,	,	PUNCT
american_scientific_journal-2511	160	51	melting	melting	NOUN
american_scientific_journal-2511	160	52	-	-	PUNCT
american_scientific_journal-2511	160	53	curve	curve	NOUN
american_scientific_journal-2511	160	54	analysis	analysis	NOUN
american_scientific_journal-2511	160	55	is	be	AUX
american_scientific_journal-2511	160	56	a	a	DET
american_scientific_journal-2511	160	57	simple	simple	ADJ
american_scientific_journal-2511	160	58	straightforward	straightforward	ADJ
american_scientific_journal-2511	160	59	way	way	NOUN
american_scientific_journal-2511	160	60	to	to	PART
american_scientific_journal-2511	160	61	check	check	VERB
american_scientific_journal-2511	160	62	real	real	ADJ
american_scientific_journal-2511	160	63	-	-	PUNCT
american_scientific_journal-2511	160	64	time	time	NOUN
american_scientific_journal-2511	160	65	pcr	pcr	ADJ
american_scientific_journal-2511	160	66	reactions	reaction	NOUN
american_scientific_journal-2511	160	67	for	for	ADP
american_scientific_journal-2511	160	68	primer	primer	NOUN
american_scientific_journal-2511	160	69	-	-	PUNCT
american_scientific_journal-2511	160	70	dimer	dimer	NOUN
american_scientific_journal-2511	160	71	artifacts	artifact	NOUN
american_scientific_journal-2511	160	72	and	and	CCONJ
american_scientific_journal-2511	160	73	to	to	PART
american_scientific_journal-2511	160	74	ensure	ensure	VERB
american_scientific_journal-2511	160	75	reaction	reaction	NOUN
american_scientific_journal-2511	160	76	specificity	specificity	NOUN
american_scientific_journal-2511	160	77	.	.	PUNCT
american_scientific_journal-2511	161	1	because	because	SCONJ
american_scientific_journal-2511	161	2	the	the	DET
american_scientific_journal-2511	161	3	melting	melting	NOUN
american_scientific_journal-2511	161	4	temperature	temperature	NOUN
american_scientific_journal-2511	161	5	of	of	ADP
american_scientific_journal-2511	161	6	nucleic	nucleic	ADJ
american_scientific_journal-2511	161	7	acids	acid	NOUN
american_scientific_journal-2511	161	8	is	be	AUX
american_scientific_journal-2511	161	9	affected	affect	VERB
american_scientific_journal-2511	161	10	by	by	ADP
american_scientific_journal-2511	161	11	length	length	NOUN
american_scientific_journal-2511	161	12	,	,	PUNCT
american_scientific_journal-2511	161	13	gc	gc	PROPN
american_scientific_journal-2511	161	14	content	content	NOUN
american_scientific_journal-2511	161	15	,	,	PUNCT
american_scientific_journal-2511	161	16	and	and	CCONJ
american_scientific_journal-2511	161	17	the	the	DET
american_scientific_journal-2511	161	18	presence	presence	NOUN
american_scientific_journal-2511	161	19	of	of	ADP
american_scientific_journal-2511	161	20	base	base	NOUN
american_scientific_journal-2511	161	21	mismatches	mismatch	NOUN
american_scientific_journal-2511	161	22	,	,	PUNCT
american_scientific_journal-2511	161	23	among	among	ADP
american_scientific_journal-2511	161	24	other	other	ADJ
american_scientific_journal-2511	161	25	factors	factor	NOUN
american_scientific_journal-2511	161	26	,	,	PUNCT
american_scientific_journal-2511	161	27	different	different	ADJ
american_scientific_journal-2511	161	28	pcr	pcr	ADJ
american_scientific_journal-2511	161	29	products	product	NOUN
american_scientific_journal-2511	161	30	can	can	AUX
american_scientific_journal-2511	161	31	often	often	ADV
american_scientific_journal-2511	161	32	be	be	AUX
american_scientific_journal-2511	161	33	distinguished	distinguish	VERB
american_scientific_journal-2511	161	34	by	by	ADP
american_scientific_journal-2511	161	35	their	their	PRON
american_scientific_journal-2511	161	36	melting	melting	NOUN
american_scientific_journal-2511	161	37	characteristics	characteristic	NOUN
american_scientific_journal-2511	161	38	,	,	PUNCT
american_scientific_journal-2511	161	39	the	the	DET
american_scientific_journal-2511	161	40	characterization	characterization	NOUN
american_scientific_journal-2511	161	41	of	of	ADP
american_scientific_journal-2511	161	42	reaction	reaction	NOUN
american_scientific_journal-2511	161	43	products	product	NOUN
american_scientific_journal-2511	161	44	[	[	X
american_scientific_journal-2511	161	45	e.g.	e.g.	ADV
american_scientific_journal-2511	161	46	,	,	PUNCT
american_scientific_journal-2511	161	47	prime	prime	ADJ
american_scientific_journal-2511	161	48	-	-	PUNCT
american_scientific_journal-2511	161	49	dimer	dimer	NOUN
american_scientific_journal-2511	161	50	vs	vs	ADP
american_scientific_journal-2511	161	51	amplicon	amplicon	NOUN
american_scientific_journal-2511	161	52	(	(	PUNCT
american_scientific_journal-2511	161	53	product	product	NOUN
american_scientific_journal-2511	161	54	)	)	PUNCT
american_scientific_journal-2511	161	55	]	]	PUNCT
american_scientific_journal-2511	161	56	via	via	ADP
american_scientific_journal-2511	161	57	melting	melt	VERB
american_scientific_journal-2511	161	58	curve	curve	NOUN
american_scientific_journal-2511	161	59	analysis	analysis	NOUN
american_scientific_journal-2511	161	60	reduces	reduce	VERB
american_scientific_journal-2511	161	61	the	the	DET
american_scientific_journal-2511	161	62	need	need	NOUN
american_scientific_journal-2511	161	63	for	for	ADP
american_scientific_journal-2511	161	64	time	time	NOUN
american_scientific_journal-2511	161	65	-	-	PUNCT
american_scientific_journal-2511	161	66	consuming	consume	VERB
american_scientific_journal-2511	161	67	gel	gel	NOUN
american_scientific_journal-2511	161	68	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2511	161	69	,	,	PUNCT
american_scientific_journal-2511	161	70	because	because	SCONJ
american_scientific_journal-2511	161	71	other	other	ADJ
american_scientific_journal-2511	161	72	product	product	NOUN
american_scientific_journal-2511	161	73	may	may	AUX
american_scientific_journal-2511	161	74	be	be	AUX
american_scientific_journal-2511	161	75	appear	appear	VERB
american_scientific_journal-2511	161	76	in	in	ADP
american_scientific_journal-2511	161	77	melting	melt	VERB
american_scientific_journal-2511	161	78	curve	curve	NOUN
american_scientific_journal-2511	161	79	but	but	CCONJ
american_scientific_journal-2511	161	80	in	in	ADP
american_scientific_journal-2511	161	81	peaks	peak	NOUN
american_scientific_journal-2511	161	82	are	be	AUX
american_scientific_journal-2511	161	83	typically	typically	ADV
american_scientific_journal-2511	161	84	in	in	ADP
american_scientific_journal-2511	161	85	a	a	DET
american_scientific_journal-2511	161	86	lower	low	ADJ
american_scientific_journal-2511	161	87	intensity	intensity	NOUN
american_scientific_journal-2511	161	88	and	and	CCONJ
american_scientific_journal-2511	161	89	represent	represent	VERB
american_scientific_journal-2511	161	90	products	product	NOUN
american_scientific_journal-2511	161	91	that	that	PRON
american_scientific_journal-2511	161	92	are	be	AUX
american_scientific_journal-2511	161	93	shorter	short	ADJ
american_scientific_journal-2511	161	94	in	in	ADP
american_scientific_journal-2511	161	95	length	length	NOUN
american_scientific_journal-2511	161	96	thus	thus	ADV
american_scientific_journal-2511	161	97	appear	appear	VERB
american_scientific_journal-2511	161	98	at	at	ADP
american_scientific_journal-2511	161	99	a	a	DET
american_scientific_journal-2511	161	100	lower	low	ADJ
american_scientific_journal-2511	161	101	temperature	temperature	NOUN
american_scientific_journal-2511	161	102	than	than	ADP
american_scientific_journal-2511	161	103	the	the	DET
american_scientific_journal-2511	161	104	target	target	NOUN
american_scientific_journal-2511	161	105	product	product	NOUN
american_scientific_journal-2511	161	106	because	because	SCONJ
american_scientific_journal-2511	161	107	tm	tm	PROPN
american_scientific_journal-2511	161	108	is	be	AUX
american_scientific_journal-2511	161	109	higher	high	ADJ
american_scientific_journal-2511	161	110	for	for	ADP
american_scientific_journal-2511	161	111	long	long	ADJ
american_scientific_journal-2511	161	112	and	and	CCONJ
american_scientific_journal-2511	161	113	in	in	ADP
american_scientific_journal-2511	161	114	gc	gc	PROPN
american_scientific_journal-2511	161	115	-	-	ADJ
american_scientific_journal-2511	161	116	rich	rich	ADJ
american_scientific_journal-2511	161	117	pcr	pcr	ADJ
american_scientific_journal-2511	161	118	product	product	NOUN
american_scientific_journal-2511	162	1	[	[	X
american_scientific_journal-2511	162	2	24,23	24,23	NOUN
american_scientific_journal-2511	162	3	]	]	PUNCT
american_scientific_journal-2511	162	4	.	.	PUNCT
american_scientific_journal-2511	163	1	a	a	DET
american_scientific_journal-2511	163	2	widely	widely	ADV
american_scientific_journal-2511	163	3	-	-	PUNCT
american_scientific_journal-2511	163	4	employed	employ	VERB
american_scientific_journal-2511	163	5	method	method	NOUN
american_scientific_journal-2511	163	6	utilizes	utilize	VERB
american_scientific_journal-2511	163	7	immobilized	immobilized	ADJ
american_scientific_journal-2511	163	8	metal	metal	NOUN
american_scientific_journal-2511	163	9	-	-	PUNCT
american_scientific_journal-2511	163	10	affinity	affinity	NOUN
american_scientific_journal-2511	163	11	chromatography	chromatography	NOUN
american_scientific_journal-2511	163	12	(	(	PUNCT
american_scientific_journal-2511	163	13	imac	imac	PROPN
american_scientific_journal-2511	163	14	)	)	PUNCT
american_scientific_journal-2511	163	15	to	to	PART
american_scientific_journal-2511	163	16	purify	purify	VERB
american_scientific_journal-2511	163	17	proteins	protein	NOUN
american_scientific_journal-2511	163	18	containing	contain	VERB
american_scientific_journal-2511	163	19	a	a	DET
american_scientific_journal-2511	163	20	short	short	ADJ
american_scientific_journal-2511	163	21	affinity	affinity	NOUN
american_scientific_journal-2511	163	22	tag	tag	NOUN
american_scientific_journal-2511	163	23	consisting	consist	VERB
american_scientific_journal-2511	163	24	of	of	ADP
american_scientific_journal-2511	163	25	histidine	histidine	NOUN
american_scientific_journal-2511	163	26	residues	residue	NOUN
american_scientific_journal-2511	163	27	(	(	PUNCT
american_scientific_journal-2511	163	28	as	as	ADP
american_scientific_journal-2511	163	29	in	in	ADP
american_scientific_journal-2511	163	30	this	this	DET
american_scientific_journal-2511	163	31	research	research	NOUN
american_scientific_journal-2511	163	32	)	)	PUNCT
american_scientific_journal-2511	163	33	.	.	PUNCT
american_scientific_journal-2511	164	1	imac	imac	PROPN
american_scientific_journal-2511	164	2	is	be	AUX
american_scientific_journal-2511	164	3	based	base	VERB
american_scientific_journal-2511	164	4	on	on	ADP
american_scientific_journal-2511	164	5	the	the	DET
american_scientific_journal-2511	164	6	interactions	interaction	NOUN
american_scientific_journal-2511	164	7	between	between	ADP
american_scientific_journal-2511	164	8	a	a	DET
american_scientific_journal-2511	164	9	transition	transition	NOUN
american_scientific_journal-2511	164	10	metal	metal	NOUN
american_scientific_journal-2511	164	11	ion	ion	NOUN
american_scientific_journal-2511	164	12	(	(	PUNCT
american_scientific_journal-2511	164	13	co2	co2	NOUN
american_scientific_journal-2511	164	14	+	+	PROPN
american_scientific_journal-2511	164	15	,	,	PUNCT
american_scientific_journal-2511	164	16	ni2	ni2	PROPN
american_scientific_journal-2511	164	17	+	+	PROPN
american_scientific_journal-2511	164	18	,	,	PUNCT
american_scientific_journal-2511	164	19	cu2	cu2	NOUN
american_scientific_journal-2511	164	20	+	+	PROPN
american_scientific_journal-2511	164	21	,	,	PUNCT
american_scientific_journal-2511	164	22	zn2	zn2	PROPN
american_scientific_journal-2511	164	23	+	+	NOUN
american_scientific_journal-2511	164	24	)	)	PUNCT
american_scientific_journal-2511	164	25	immobilized	immobilize	VERB
american_scientific_journal-2511	164	26	on	on	ADP
american_scientific_journal-2511	164	27	a	a	DET
american_scientific_journal-2511	164	28	matrix	matrix	NOUN
american_scientific_journal-2511	164	29	and	and	CCONJ
american_scientific_journal-2511	164	30	specific	specific	ADJ
american_scientific_journal-2511	164	31	amino	amino	NOUN
american_scientific_journal-2511	164	32	acid	acid	NOUN
american_scientific_journal-2511	164	33	side	side	NOUN
american_scientific_journal-2511	164	34	.	.	PUNCT
american_scientific_journal-2511	165	1	histidine	histidine	NOUN
american_scientific_journal-2511	165	2	is	be	AUX
american_scientific_journal-2511	165	3	the	the	DET
american_scientific_journal-2511	165	4	amino	amino	NOUN
american_scientific_journal-2511	165	5	acid	acid	NOUN
american_scientific_journal-2511	165	6	that	that	PRON
american_scientific_journal-2511	165	7	exhibits	exhibit	VERB
american_scientific_journal-2511	165	8	the	the	DET
american_scientific_journal-2511	165	9	strongest	strong	ADJ
american_scientific_journal-2511	165	10	interaction	interaction	NOUN
american_scientific_journal-2511	165	11	with	with	ADP
american_scientific_journal-2511	165	12	immobilized	immobilized	ADJ
american_scientific_journal-2511	165	13	metal	metal	NOUN
american_scientific_journal-2511	165	14	ion	ion	NOUN
american_scientific_journal-2511	165	15	matrices	matrix	NOUN
american_scientific_journal-2511	165	16	,	,	PUNCT
american_scientific_journal-2511	165	17	as	as	SCONJ
american_scientific_journal-2511	165	18	electron	electron	NOUN
american_scientific_journal-2511	165	19	donor	donor	NOUN
american_scientific_journal-2511	165	20	groups	group	NOUN
american_scientific_journal-2511	165	21	on	on	ADP
american_scientific_journal-2511	165	22	the	the	DET
american_scientific_journal-2511	165	23	histidine	histidine	NOUN
american_scientific_journal-2511	165	24	imidazole	imidazole	NOUN
american_scientific_journal-2511	165	25	ring	ring	NOUN
american_scientific_journal-2511	165	26	readily	readily	ADV
american_scientific_journal-2511	165	27	form	form	NOUN
american_scientific_journal-2511	165	28	coordination	coordination	NOUN
american_scientific_journal-2511	165	29	bonds	bond	NOUN
american_scientific_journal-2511	165	30	with	with	ADP
american_scientific_journal-2511	165	31	the	the	DET
american_scientific_journal-2511	165	32	immobilized	immobilized	ADJ
american_scientific_journal-2511	165	33	transition	transition	NOUN
american_scientific_journal-2511	165	34	metal	metal	NOUN
american_scientific_journal-2511	165	35	.	.	PUNCT
american_scientific_journal-2511	166	1	peptides	peptide	NOUN
american_scientific_journal-2511	166	2	containing	contain	VERB
american_scientific_journal-2511	166	3	sequences	sequence	NOUN
american_scientific_journal-2511	166	4	of	of	ADP
american_scientific_journal-2511	166	5	consecutive	consecutive	ADJ
american_scientific_journal-2511	166	6	histidine	histidine	NOUN
american_scientific_journal-2511	166	7	residues	residue	NOUN
american_scientific_journal-2511	166	8	are	be	AUX
american_scientific_journal-2511	166	9	efficiently	efficiently	ADV
american_scientific_journal-2511	166	10	retained	retain	VERB
american_scientific_journal-2511	166	11	on	on	ADP
american_scientific_journal-2511	166	12	imac	imac	NOUN
american_scientific_journal-2511	166	13	column	column	NOUN
american_scientific_journal-2511	166	14	matrices	matrix	NOUN
american_scientific_journal-2511	166	15	.	.	PUNCT
american_scientific_journal-2511	167	1	following	follow	VERB
american_scientific_journal-2511	167	2	washing	washing	NOUN
american_scientific_journal-2511	167	3	of	of	ADP
american_scientific_journal-2511	167	4	the	the	DET
american_scientific_journal-2511	167	5	matrix	matrix	NOUN
american_scientific_journal-2511	167	6	material	material	NOUN
american_scientific_journal-2511	167	7	,	,	PUNCT
american_scientific_journal-2511	167	8	peptides	peptide	NOUN
american_scientific_journal-2511	167	9	containing	contain	VERB
american_scientific_journal-2511	167	10	polyhistidine	polyhistidine	NOUN
american_scientific_journal-2511	167	11	sequences	sequence	NOUN
american_scientific_journal-2511	167	12	can	can	AUX
american_scientific_journal-2511	167	13	be	be	AUX
american_scientific_journal-2511	167	14	easily	easily	ADV
american_scientific_journal-2511	167	15	eluted	elute	VERB
american_scientific_journal-2511	167	16	by	by	ADP
american_scientific_journal-2511	167	17	either	either	CCONJ
american_scientific_journal-2511	167	18	adjusting	adjust	VERB
american_scientific_journal-2511	167	19	the	the	DET
american_scientific_journal-2511	167	20	ph	ph	NOUN
american_scientific_journal-2511	167	21	of	of	ADP
american_scientific_journal-2511	167	22	the	the	DET
american_scientific_journal-2511	167	23	column	column	NOUN
american_scientific_journal-2511	167	24	buffer	buffer	NOUN
american_scientific_journal-2511	167	25	or	or	CCONJ
american_scientific_journal-2511	167	26	adding	add	VERB
american_scientific_journal-2511	167	27	free	free	ADJ
american_scientific_journal-2511	167	28	imidazole	imidazole	NOUN
american_scientific_journal-2511	167	29	to	to	ADP
american_scientific_journal-2511	167	30	the	the	DET
american_scientific_journal-2511	167	31	column	column	NOUN
american_scientific_journal-2511	167	32	buffer	buffer	NOUN
american_scientific_journal-2511	167	33	[	[	X
american_scientific_journal-2511	167	34	25	25	NUM
american_scientific_journal-2511	167	35	]	]	PUNCT
american_scientific_journal-2511	167	36	.	.	PUNCT
american_scientific_journal-2511	168	1	in	in	ADP
american_scientific_journal-2511	168	2	this	this	DET
american_scientific_journal-2511	168	3	study	study	NOUN
american_scientific_journal-2511	168	4	target	target	NOUN
american_scientific_journal-2511	168	5	protein	protein	NOUN
american_scientific_journal-2511	168	6	contain	contain	VERB
american_scientific_journal-2511	168	7	10	10	NUM
american_scientific_journal-2511	168	8	-	-	PUNCT
american_scientific_journal-2511	168	9	histidine	histidine	ADJ
american_scientific_journal-2511	168	10	and	and	CCONJ
american_scientific_journal-2511	168	11	macroporous	macroporous	ADJ
american_scientific_journal-2511	168	12	silica	silica	NOUN
american_scientific_journal-2511	168	13	resin	resin	NOUN
american_scientific_journal-2511	168	14	modified	modify	VERB
american_scientific_journal-2511	168	15	to	to	PART
american_scientific_journal-2511	168	16	contain	contain	VERB
american_scientific_journal-2511	168	17	a	a	DET
american_scientific_journal-2511	168	18	high	high	ADJ
american_scientific_journal-2511	168	19	level	level	NOUN
american_scientific_journal-2511	168	20	of	of	ADP
american_scientific_journal-2511	168	21	tetradentate	tetradentate	NOUN
american_scientific_journal-2511	168	22	-	-	PUNCT
american_scientific_journal-2511	168	23	chelated	chelate	VERB
american_scientific_journal-2511	168	24	nickel	nickel	NOUN
american_scientific_journal-2511	168	25	was	be	AUX
american_scientific_journal-2511	168	26	used	use	VERB
american_scientific_journal-2511	168	27	for	for	ADP
american_scientific_journal-2511	168	28	efficient	efficient	ADJ
american_scientific_journal-2511	168	29	capture	capture	NOUN
american_scientific_journal-2511	168	30	and	and	CCONJ
american_scientific_journal-2511	168	31	purification	purification	NOUN
american_scientific_journal-2511	168	32	of	of	ADP
american_scientific_journal-2511	168	33	bacterially	bacterially	ADV
american_scientific_journal-2511	168	34	expressed	express	VERB
american_scientific_journal-2511	168	35	his	his	PRON
american_scientific_journal-2511	168	36	-	-	PUNCT
american_scientific_journal-2511	168	37	tag	tag	NOUN
american_scientific_journal-2511	168	38	protein	protein	NOUN
american_scientific_journal-2511	168	39	then	then	ADV
american_scientific_journal-2511	168	40	target	target	VERB
american_scientific_journal-2511	168	41	protein	protein	NOUN
american_scientific_journal-2511	168	42	eluted	elute	VERB
american_scientific_journal-2511	168	43	by	by	ADP
american_scientific_journal-2511	168	44	adding	add	VERB
american_scientific_journal-2511	168	45	imidazole	imidazole	NOUN
american_scientific_journal-2511	168	46	to	to	ADP
american_scientific_journal-2511	168	47	the	the	DET
american_scientific_journal-2511	168	48	column	column	NOUN
american_scientific_journal-2511	168	49	buffer	buffer	NOUN
american_scientific_journal-2511	169	1	[	[	X
american_scientific_journal-2511	169	2	26	26	NUM
american_scientific_journal-2511	169	3	]	]	PUNCT
american_scientific_journal-2511	169	4	.	.	PUNCT
american_scientific_journal-2511	170	1	removal	removal	NOUN
american_scientific_journal-2511	170	2	of	of	ADP
american_scientific_journal-2511	170	3	the	the	DET
american_scientific_journal-2511	170	4	tag	tag	NOUN
american_scientific_journal-2511	170	5	from	from	ADP
american_scientific_journal-2511	170	6	a	a	DET
american_scientific_journal-2511	170	7	protein	protein	NOUN
american_scientific_journal-2511	170	8	of	of	ADP
american_scientific_journal-2511	170	9	interest	interest	NOUN
american_scientific_journal-2511	170	10	can	can	AUX
american_scientific_journal-2511	170	11	be	be	AUX
american_scientific_journal-2511	170	12	accomplished	accomplish	VERB
american_scientific_journal-2511	170	13	with	with	ADP
american_scientific_journal-2511	170	14	a	a	DET
american_scientific_journal-2511	170	15	site	site	NOUN
american_scientific_journal-2511	170	16	-	-	PUNCT
american_scientific_journal-2511	170	17	specific	specific	ADJ
american_scientific_journal-2511	170	18	protease	protease	NOUN
american_scientific_journal-2511	170	19	and	and	CCONJ
american_scientific_journal-2511	170	20	cleavage	cleavage	NOUN
american_scientific_journal-2511	170	21	should	should	AUX
american_scientific_journal-2511	170	22	not	not	PART
american_scientific_journal-2511	170	23	reduce	reduce	VERB
american_scientific_journal-2511	170	24	protein	protein	NOUN
american_scientific_journal-2511	170	25	activity	activity	NOUN
american_scientific_journal-2511	170	26	.	.	PUNCT
american_scientific_journal-2511	171	1	factor	factor	NOUN
american_scientific_journal-2511	171	2	xa	xa	PROPN
american_scientific_journal-2511	171	3	is	be	AUX
american_scientific_journal-2511	171	4	protease	protease	NOUN
american_scientific_journal-2511	171	5	was	be	AUX
american_scientific_journal-2511	171	6	used	use	VERB
american_scientific_journal-2511	171	7	in	in	ADP
american_scientific_journal-2511	171	8	this	this	DET
american_scientific_journal-2511	171	9	study	study	NOUN
american_scientific_journal-2511	171	10	to	to	PART
american_scientific_journal-2511	171	11	cleave	cleave	VERB
american_scientific_journal-2511	171	12	his	his	PRON
american_scientific_journal-2511	171	13	-	-	PUNCT
american_scientific_journal-2511	171	14	tag	tag	NOUN
american_scientific_journal-2511	171	15	from	from	ADP
american_scientific_journal-2511	171	16	target	target	NOUN
american_scientific_journal-2511	171	17	protein	protein	NOUN
american_scientific_journal-2511	171	18	,	,	PUNCT
american_scientific_journal-2511	171	19	this	this	DET
american_scientific_journal-2511	171	20	protease	protease	NOUN
american_scientific_journal-2511	171	21	recognizes	recognize	VERB
american_scientific_journal-2511	171	22	a	a	DET
american_scientific_journal-2511	171	23	site	site	NOUN
american_scientific_journal-2511	171	24	between	between	ADP
american_scientific_journal-2511	171	25	target	target	NOUN
american_scientific_journal-2511	171	26	protein	protein	NOUN
american_scientific_journal-2511	171	27	and	and	CCONJ
american_scientific_journal-2511	171	28	his	his	PRON
american_scientific_journal-2511	171	29	-	-	PUNCT
american_scientific_journal-2511	171	30	tag	tag	NOUN
american_scientific_journal-2511	171	31	in	in	ADP
american_scientific_journal-2511	171	32	n	n	CCONJ
american_scientific_journal-2511	171	33	-	-	PUNCT
american_scientific_journal-2511	171	34	terminal	terminal	NOUN
american_scientific_journal-2511	171	35	the	the	DET
american_scientific_journal-2511	171	36	recognition	recognition	NOUN
american_scientific_journal-2511	171	37	site	site	NOUN
american_scientific_journal-2511	171	38	consists	consist	VERB
american_scientific_journal-2511	171	39	from	from	ADP
american_scientific_journal-2511	171	40	the	the	DET
american_scientific_journal-2511	171	41	flowing	flow	VERB
american_scientific_journal-2511	171	42	amino	amino	NOUN
american_scientific_journal-2511	171	43	acid	acid	NOUN
american_scientific_journal-2511	171	44	(	(	PUNCT
american_scientific_journal-2511	171	45	isoleucin	isoleucin	PROPN
american_scientific_journal-2511	171	46	glutamic	glutamic	ADJ
american_scientific_journal-2511	171	47	acid	acid	NOUN
american_scientific_journal-2511	171	48	–	–	PUNCT
american_scientific_journal-2511	171	49	glycin	glycin	VERB
american_scientific_journal-2511	171	50	-arginin	-arginin	PROPN
american_scientific_journal-2511	171	51	)	)	PUNCT
american_scientific_journal-2511	171	52	,	,	PUNCT
american_scientific_journal-2511	171	53	and	and	CCONJ
american_scientific_journal-2511	171	54	cleaves	cleave	VERB
american_scientific_journal-2511	171	55	after	after	ADP
american_scientific_journal-2511	171	56	the	the	DET
american_scientific_journal-2511	171	57	carboxyl	carboxyl	NOUN
american_scientific_journal-2511	171	58	arginine	arginine	NOUN
american_scientific_journal-2511	171	59	american	american	PROPN
american_scientific_journal-2511	171	60	scientific	scientific	PROPN
american_scientific_journal-2511	171	61	research	research	PROPN
american_scientific_journal-2511	171	62	journal	journal	NOUN
american_scientific_journal-2511	171	63	for	for	ADP
american_scientific_journal-2511	171	64	engineering	engineering	NOUN
american_scientific_journal-2511	171	65	,	,	PUNCT
american_scientific_journal-2511	171	66	technology	technology	NOUN
american_scientific_journal-2511	171	67	,	,	PUNCT
american_scientific_journal-2511	171	68	and	and	CCONJ
american_scientific_journal-2511	171	69	sciences	science	NOUN
american_scientific_journal-2511	171	70	(	(	PUNCT
american_scientific_journal-2511	171	71	asrjets	asrjet	NOUN
american_scientific_journal-2511	171	72	)	)	PUNCT
american_scientific_journal-2511	171	73	(	(	PUNCT
american_scientific_journal-2511	171	74	2017	2017	NUM
american_scientific_journal-2511	171	75	)	)	PUNCT
american_scientific_journal-2511	171	76	volume	volume	NOUN
american_scientific_journal-2511	171	77	27	27	NUM
american_scientific_journal-2511	171	78	,	,	PUNCT
american_scientific_journal-2511	171	79	no	no	DET
american_scientific_journal-2511	171	80	1	1	NUM
american_scientific_journal-2511	171	81	,	,	PUNCT
american_scientific_journal-2511	171	82	pp	pp	ADV
american_scientific_journal-2511	171	83	25	25	NUM
american_scientific_journal-2511	171	84	-	-	SYM
american_scientific_journal-2511	171	85	42	42	NUM
american_scientific_journal-2511	171	86	40	40	NUM
american_scientific_journal-2511	172	1	[	[	X
american_scientific_journal-2511	172	2	27,28	27,28	NUM
american_scientific_journal-2511	172	3	]	]	X
american_scientific_journal-2511	172	4	.	.	PUNCT
american_scientific_journal-2511	173	1	this	this	DET
american_scientific_journal-2511	173	2	treatment	treatment	NOUN
american_scientific_journal-2511	173	3	reduces	reduce	VERB
american_scientific_journal-2511	173	4	the	the	DET
american_scientific_journal-2511	173	5	molecular	molecular	ADJ
american_scientific_journal-2511	173	6	weight	weight	NOUN
american_scientific_journal-2511	173	7	of	of	ADP
american_scientific_journal-2511	173	8	target	target	NOUN
american_scientific_journal-2511	173	9	protein	protein	NOUN
american_scientific_journal-2511	173	10	by	by	ADP
american_scientific_journal-2511	173	11	removing	remove	VERB
american_scientific_journal-2511	173	12	his	his	PRON
american_scientific_journal-2511	173	13	-	-	PUNCT
american_scientific_journal-2511	173	14	tag	tag	NOUN
american_scientific_journal-2511	173	15	[	[	PUNCT
american_scientific_journal-2511	173	16	molecular	molecular	ADJ
american_scientific_journal-2511	173	17	weight	weight	NOUN
american_scientific_journal-2511	173	18	for	for	ADP
american_scientific_journal-2511	173	19	hirudin	hirudin	VERB
american_scientific_journal-2511	173	20	protein	protein	NOUN
american_scientific_journal-2511	173	21	become	become	VERB
american_scientific_journal-2511	173	22	~9.2	~9.2	X
american_scientific_journal-2511	173	23	kda	kda	NOUN
american_scientific_journal-2511	173	24	.	.	PUNCT
american_scientific_journal-2511	173	25	]	]	PUNCT
american_scientific_journal-2511	173	26	.	.	PUNCT
american_scientific_journal-2511	174	1	then	then	ADV
american_scientific_journal-2511	174	2	this	this	DET
american_scientific_journal-2511	174	3	protein	protein	NOUN
american_scientific_journal-2511	174	4	purified	purify	VERB
american_scientific_journal-2511	174	5	by	by	ADP
american_scientific_journal-2511	174	6	using	use	VERB
american_scientific_journal-2511	174	7	deae	deae	NOUN
american_scientific_journal-2511	174	8	sepharose	sepharose	NOUN
american_scientific_journal-2511	174	9	(	(	PUNCT
american_scientific_journal-2511	174	10	anion	anion	NOUN
american_scientific_journal-2511	174	11	exchange	exchange	NOUN
american_scientific_journal-2511	174	12	chromatography	chromatography	NOUN
american_scientific_journal-2511	174	13	)	)	PUNCT
american_scientific_journal-2511	174	14	.	.	PUNCT
american_scientific_journal-2511	175	1	in	in	ADP
american_scientific_journal-2511	175	2	deae	deae	NOUN
american_scientific_journal-2511	175	3	sepharose	sepharose	NOUN
american_scientific_journal-2511	175	4	target	target	NOUN
american_scientific_journal-2511	175	5	protein	protein	NOUN
american_scientific_journal-2511	175	6	eluents	eluent	NOUN
american_scientific_journal-2511	175	7	by	by	ADP
american_scientific_journal-2511	175	8	using	use	VERB
american_scientific_journal-2511	175	9	salt	salt	NOUN
american_scientific_journal-2511	175	10	(	(	PUNCT
american_scientific_journal-2511	175	11	nacl	nacl	NOUN
american_scientific_journal-2511	175	12	)	)	PUNCT
american_scientific_journal-2511	175	13	,	,	PUNCT
american_scientific_journal-2511	175	14	this	this	DET
american_scientific_journal-2511	175	15	salt	salt	NOUN
american_scientific_journal-2511	175	16	is	be	AUX
american_scientific_journal-2511	175	17	probably	probably	ADV
american_scientific_journal-2511	175	18	the	the	DET
american_scientific_journal-2511	175	19	most	most	ADV
american_scientific_journal-2511	175	20	widely	widely	ADV
american_scientific_journal-2511	175	21	used	use	VERB
american_scientific_journal-2511	175	22	and	and	CCONJ
american_scientific_journal-2511	175	23	mild	mild	ADJ
american_scientific_journal-2511	175	24	eluent	eluent	NOUN
american_scientific_journal-2511	175	25	for	for	ADP
american_scientific_journal-2511	175	26	protein	protein	NOUN
american_scientific_journal-2511	175	27	separation	separation	NOUN
american_scientific_journal-2511	175	28	due	due	ADJ
american_scientific_journal-2511	175	29	to	to	ADP
american_scientific_journal-2511	175	30	has	have	AUX
american_scientific_journal-2511	175	31	no	no	DET
american_scientific_journal-2511	175	32	important	important	ADJ
american_scientific_journal-2511	175	33	effect	effect	NOUN
american_scientific_journal-2511	175	34	on	on	ADP
american_scientific_journal-2511	175	35	protein	protein	NOUN
american_scientific_journal-2511	175	36	structure	structure	NOUN
american_scientific_journal-2511	175	37	[	[	X
american_scientific_journal-2511	175	38	29	29	NUM
american_scientific_journal-2511	175	39	]	]	PUNCT
american_scientific_journal-2511	175	40	.	.	PUNCT
american_scientific_journal-2511	176	1	in	in	ADP
american_scientific_journal-2511	176	2	this	this	DET
american_scientific_journal-2511	176	3	study	study	NOUN
american_scientific_journal-2511	176	4	a	a	DET
american_scientific_journal-2511	176	5	linear	linear	ADJ
american_scientific_journal-2511	176	6	salt	salt	NOUN
american_scientific_journal-2511	176	7	gradients	gradient	NOUN
american_scientific_journal-2511	176	8	were	be	AUX
american_scientific_journal-2511	176	9	used	use	VERB
american_scientific_journal-2511	176	10	from	from	ADP
american_scientific_journal-2511	176	11	(	(	PUNCT
american_scientific_journal-2511	176	12	0.1	0.1	NUM
american_scientific_journal-2511	176	13	-	-	SYM
american_scientific_journal-2511	176	14	0.5	0.5	NUM
american_scientific_journal-2511	176	15	m	m	NOUN
american_scientific_journal-2511	176	16	)	)	PUNCT
american_scientific_journal-2511	176	17	in	in	ADP
american_scientific_journal-2511	176	18	elution	elution	NOUN
american_scientific_journal-2511	176	19	buffer	buffer	NOUN
american_scientific_journal-2511	176	20	the	the	DET
american_scientific_journal-2511	176	21	target	target	NOUN
american_scientific_journal-2511	176	22	protein	protein	NOUN
american_scientific_journal-2511	176	23	band	band	NOUN
american_scientific_journal-2511	176	24	appear	appear	VERB
american_scientific_journal-2511	176	25	in	in	ADP
american_scientific_journal-2511	176	26	concentration	concentration	NOUN
american_scientific_journal-2511	176	27	(	(	PUNCT
american_scientific_journal-2511	176	28	0.2	0.2	NUM
american_scientific_journal-2511	176	29	-	-	PUNCT
american_scientific_journal-2511	176	30	0.4	0.4	NUM
american_scientific_journal-2511	176	31	m	m	NOUN
american_scientific_journal-2511	176	32	)	)	PUNCT
american_scientific_journal-2511	177	1	[	[	X
american_scientific_journal-2511	177	2	30	30	NUM
american_scientific_journal-2511	177	3	]	]	PUNCT
american_scientific_journal-2511	177	4	,	,	PUNCT
american_scientific_journal-2511	177	5	by	by	ADP
american_scientific_journal-2511	177	6	using	use	VERB
american_scientific_journal-2511	177	7	tris	tris	ADJ
american_scientific_journal-2511	177	8	-	-	PUNCT
american_scientific_journal-2511	177	9	hcl	hcl	NOUN
american_scientific_journal-2511	177	10	buffer	buffer	NOUN
american_scientific_journal-2511	177	11	with	with	ADP
american_scientific_journal-2511	177	12	ph=8	ph=8	PROPN
american_scientific_journal-2511	177	13	(	(	PUNCT
american_scientific_journal-2511	177	14	20	20	NUM
american_scientific_journal-2511	177	15	mm	mm	NOUN
american_scientific_journal-2511	177	16	)	)	PUNCT
american_scientific_journal-2511	177	17	in	in	ADP
american_scientific_journal-2511	177	18	binding	bind	VERB
american_scientific_journal-2511	177	19	and	and	CCONJ
american_scientific_journal-2511	177	20	washing	washing	NOUN
american_scientific_journal-2511	177	21	buffer	buffer	NOUN
american_scientific_journal-2511	177	22	without	without	ADP
american_scientific_journal-2511	177	23	nacl	nacl	NOUN
american_scientific_journal-2511	177	24	then	then	ADV
american_scientific_journal-2511	177	25	elute	elute	VERB
american_scientific_journal-2511	177	26	protein	protein	NOUN
american_scientific_journal-2511	177	27	with	with	ADP
american_scientific_journal-2511	177	28	gradient	gradient	ADJ
american_scientific_journal-2511	177	29	nacl	nacl	NOUN
american_scientific_journal-2511	177	30	allowing	allow	VERB
american_scientific_journal-2511	177	31	counter	counter	NOUN
american_scientific_journal-2511	177	32	-	-	NOUN
american_scientific_journal-2511	177	33	ion	ion	NOUN
american_scientific_journal-2511	177	34	clto	clto	NOUN
american_scientific_journal-2511	177	35	bind	bind	VERB
american_scientific_journal-2511	177	36	to	to	ADP
american_scientific_journal-2511	177	37	the	the	DET
american_scientific_journal-2511	177	38	deae	deae	NOUN
american_scientific_journal-2511	177	39	exchanger	exchanger	NOUN
american_scientific_journal-2511	177	40	and	and	CCONJ
american_scientific_journal-2511	177	41	elute	elute	NOUN
american_scientific_journal-2511	177	42	target	target	NOUN
american_scientific_journal-2511	177	43	protein	protein	NOUN
american_scientific_journal-2511	177	44	.	.	PUNCT
american_scientific_journal-2511	178	1	sp	sp	ADP
american_scientific_journal-2511	178	2	sepharose	sepharose	NOUN
american_scientific_journal-2511	178	3	is	be	AUX
american_scientific_journal-2511	178	4	cation	cation	NOUN
american_scientific_journal-2511	178	5	exchanger	exchanger	NOUN
american_scientific_journal-2511	178	6	with	with	ADP
american_scientific_journal-2511	178	7	negatively	negatively	ADV
american_scientific_journal-2511	178	8	charge	charge	VERB
american_scientific_journal-2511	178	9	functional	functional	ADJ
american_scientific_journal-2511	178	10	group	group	NOUN
american_scientific_journal-2511	178	11	,	,	PUNCT
american_scientific_journal-2511	178	12	target	target	VERB
american_scientific_journal-2511	178	13	protein	protein	NOUN
american_scientific_journal-2511	178	14	eluent	eluent	NOUN
american_scientific_journal-2511	178	15	from	from	ADP
american_scientific_journal-2511	178	16	this	this	DET
american_scientific_journal-2511	178	17	column	column	NOUN
american_scientific_journal-2511	178	18	depending	depend	VERB
american_scientific_journal-2511	178	19	on	on	ADP
american_scientific_journal-2511	178	20	iso	iso	NOUN
american_scientific_journal-2511	178	21	-	-	PUNCT
american_scientific_journal-2511	178	22	electric	electric	ADJ
american_scientific_journal-2511	178	23	point	point	NOUN
american_scientific_journal-2511	178	24	(	(	PUNCT
american_scientific_journal-2511	178	25	pi	pi	NOUN
american_scientific_journal-2511	178	26	)	)	PUNCT
american_scientific_journal-2511	178	27	which	which	PRON
american_scientific_journal-2511	178	28	is	be	AUX
american_scientific_journal-2511	178	29	the	the	DET
american_scientific_journal-2511	178	30	ph	ph	NOUN
american_scientific_journal-2511	178	31	at	at	ADP
american_scientific_journal-2511	178	32	which	which	PRON
american_scientific_journal-2511	178	33	the	the	DET
american_scientific_journal-2511	178	34	net	net	ADJ
american_scientific_journal-2511	178	35	charge	charge	NOUN
american_scientific_journal-2511	178	36	of	of	ADP
american_scientific_journal-2511	178	37	the	the	DET
american_scientific_journal-2511	178	38	protein	protein	NOUN
american_scientific_journal-2511	178	39	is	be	AUX
american_scientific_journal-2511	178	40	neutral	neutral	ADJ
american_scientific_journal-2511	178	41	(	(	PUNCT
american_scientific_journal-2511	178	42	the	the	DET
american_scientific_journal-2511	178	43	number	number	NOUN
american_scientific_journal-2511	178	44	of	of	ADP
american_scientific_journal-2511	178	45	positive	positive	ADJ
american_scientific_journal-2511	178	46	charges	charge	NOUN
american_scientific_journal-2511	178	47	is	be	AUX
american_scientific_journal-2511	178	48	equal	equal	ADJ
american_scientific_journal-2511	178	49	to	to	ADP
american_scientific_journal-2511	178	50	the	the	DET
american_scientific_journal-2511	178	51	number	number	NOUN
american_scientific_journal-2511	178	52	of	of	ADP
american_scientific_journal-2511	178	53	negative	negative	ADJ
american_scientific_journal-2511	178	54	charges	charge	NOUN
american_scientific_journal-2511	178	55	)	)	PUNCT
american_scientific_journal-2511	178	56	.	.	PUNCT
american_scientific_journal-2511	179	1	the	the	DET
american_scientific_journal-2511	179	2	result	result	NOUN
american_scientific_journal-2511	179	3	for	for	ADP
american_scientific_journal-2511	179	4	sp	sp	ADP
american_scientific_journal-2511	179	5	sepharose	sepharose	NOUN
american_scientific_journal-2511	179	6	illustrate	illustrate	VERB
american_scientific_journal-2511	179	7	in	in	ADP
american_scientific_journal-2511	179	8	figure	figure	NOUN
american_scientific_journal-2511	179	9	12	12	NUM
american_scientific_journal-2511	179	10	,	,	PUNCT
american_scientific_journal-2511	179	11	hirudin	hirudin	VERB
american_scientific_journal-2511	179	12	protein	protein	NOUN
american_scientific_journal-2511	179	13	eluted	elute	VERB
american_scientific_journal-2511	179	14	in	in	ADP
american_scientific_journal-2511	179	15	buffer	buffer	NOUN
american_scientific_journal-2511	179	16	(	(	PUNCT
american_scientific_journal-2511	179	17	ph=8	ph=8	NOUN
american_scientific_journal-2511	179	18	)	)	PUNCT
american_scientific_journal-2511	180	1	[	[	X
american_scientific_journal-2511	180	2	31	31	NUM
american_scientific_journal-2511	180	3	]	]	PUNCT
american_scientific_journal-2511	180	4	.	.	PUNCT
american_scientific_journal-2511	181	1	the	the	DET
american_scientific_journal-2511	181	2	biological	biological	ADJ
american_scientific_journal-2511	181	3	activity	activity	NOUN
american_scientific_journal-2511	181	4	of	of	ADP
american_scientific_journal-2511	181	5	hirudin	hirudin	VERB
american_scientific_journal-2511	181	6	conducted	conduct	VERB
american_scientific_journal-2511	181	7	by	by	ADP
american_scientific_journal-2511	181	8	thrombin	thrombin	PROPN
american_scientific_journal-2511	181	9	titration	titration	PROPN
american_scientific_journal-2511	181	10	.	.	PUNCT
american_scientific_journal-2511	182	1	titration	titration	NOUN
american_scientific_journal-2511	182	2	is	be	AUX
american_scientific_journal-2511	182	3	an	an	DET
american_scientific_journal-2511	182	4	analytical	analytical	ADJ
american_scientific_journal-2511	182	5	technique	technique	NOUN
american_scientific_journal-2511	182	6	which	which	PRON
american_scientific_journal-2511	182	7	allows	allow	VERB
american_scientific_journal-2511	182	8	the	the	DET
american_scientific_journal-2511	182	9	quantitative	quantitative	ADJ
american_scientific_journal-2511	182	10	determination	determination	NOUN
american_scientific_journal-2511	182	11	of	of	ADP
american_scientific_journal-2511	182	12	a	a	DET
american_scientific_journal-2511	182	13	specific	specific	ADJ
american_scientific_journal-2511	182	14	substance	substance	NOUN
american_scientific_journal-2511	182	15	(	(	PUNCT
american_scientific_journal-2511	182	16	analyte	analyte	NOUN
american_scientific_journal-2511	182	17	)	)	PUNCT
american_scientific_journal-2511	182	18	dissolved	dissolve	VERB
american_scientific_journal-2511	182	19	in	in	ADP
american_scientific_journal-2511	182	20	a	a	DET
american_scientific_journal-2511	182	21	sample	sample	NOUN
american_scientific_journal-2511	182	22	.	.	PUNCT
american_scientific_journal-2511	183	1	it	it	PRON
american_scientific_journal-2511	183	2	is	be	AUX
american_scientific_journal-2511	183	3	based	base	VERB
american_scientific_journal-2511	183	4	on	on	ADP
american_scientific_journal-2511	183	5	a	a	DET
american_scientific_journal-2511	183	6	complete	complete	ADJ
american_scientific_journal-2511	183	7	chemical	chemical	NOUN
american_scientific_journal-2511	183	8	reaction	reaction	NOUN
american_scientific_journal-2511	183	9	between	between	ADP
american_scientific_journal-2511	183	10	the	the	DET
american_scientific_journal-2511	183	11	analyte	analyte	NOUN
american_scientific_journal-2511	183	12	and	and	CCONJ
american_scientific_journal-2511	183	13	a	a	DET
american_scientific_journal-2511	183	14	reagent	reagent	ADJ
american_scientific_journal-2511	183	15	(	(	PUNCT
american_scientific_journal-2511	183	16	titrant	titrant	NOUN
american_scientific_journal-2511	183	17	)	)	PUNCT
american_scientific_journal-2511	183	18	of	of	ADP
american_scientific_journal-2511	183	19	known	know	VERB
american_scientific_journal-2511	183	20	concentration	concentration	NOUN
american_scientific_journal-2511	183	21	which	which	PRON
american_scientific_journal-2511	183	22	is	be	AUX
american_scientific_journal-2511	183	23	added	add	VERB
american_scientific_journal-2511	183	24	to	to	ADP
american_scientific_journal-2511	183	25	the	the	DET
american_scientific_journal-2511	183	26	sample	sample	NOUN
american_scientific_journal-2511	183	27	.	.	PUNCT
american_scientific_journal-2511	184	1	during	during	ADP
american_scientific_journal-2511	184	2	this	this	DET
american_scientific_journal-2511	184	3	experiment	experiment	NOUN
american_scientific_journal-2511	184	4	a	a	DET
american_scientific_journal-2511	184	5	specific	specific	ADJ
american_scientific_journal-2511	184	6	,	,	PUNCT
american_scientific_journal-2511	184	7	rapid	rapid	ADJ
american_scientific_journal-2511	184	8	,	,	PUNCT
american_scientific_journal-2511	184	9	and	and	CCONJ
american_scientific_journal-2511	184	10	stoichiometric	stoichiometric	ADJ
american_scientific_journal-2511	184	11	reaction	reaction	NOUN
american_scientific_journal-2511	184	12	between	between	ADP
american_scientific_journal-2511	184	13	hirudin	hirudin	NOUN
american_scientific_journal-2511	184	14	and	and	CCONJ
american_scientific_journal-2511	184	15	thrombin	thrombin	NOUN
american_scientific_journal-2511	184	16	,	,	PUNCT
american_scientific_journal-2511	184	17	hirudin	hirudin	VERB
american_scientific_journal-2511	184	18	activity	activity	NOUN
american_scientific_journal-2511	184	19	can	can	AUX
american_scientific_journal-2511	184	20	be	be	AUX
american_scientific_journal-2511	184	21	quantitatively	quantitatively	ADV
american_scientific_journal-2511	184	22	determined	determine	VERB
american_scientific_journal-2511	184	23	by	by	ADP
american_scientific_journal-2511	184	24	titration	titration	NOUN
american_scientific_journal-2511	184	25	with	with	ADP
american_scientific_journal-2511	184	26	a	a	DET
american_scientific_journal-2511	184	27	standardized	standardized	ADJ
american_scientific_journal-2511	184	28	thrombin	thrombin	NOUN
american_scientific_journal-2511	184	29	solution	solution	NOUN
american_scientific_journal-2511	184	30	.	.	PUNCT
american_scientific_journal-2511	185	1	the	the	DET
american_scientific_journal-2511	185	2	principle	principle	NOUN
american_scientific_journal-2511	185	3	involves	involve	VERB
american_scientific_journal-2511	185	4	a	a	DET
american_scientific_journal-2511	185	5	fibrinogen	fibrinogen	NOUN
american_scientific_journal-2511	185	6	solution	solution	NOUN
american_scientific_journal-2511	185	7	to	to	PART
american_scientific_journal-2511	185	8	which	which	PRON
american_scientific_journal-2511	185	9	hirudin	hirudin	VERB
american_scientific_journal-2511	185	10	was	be	AUX
american_scientific_journal-2511	185	11	added	add	VERB
american_scientific_journal-2511	185	12	will	will	AUX
american_scientific_journal-2511	185	13	not	not	PART
american_scientific_journal-2511	185	14	clot	clot	VERB
american_scientific_journal-2511	185	15	until	until	SCONJ
american_scientific_journal-2511	185	16	enough	enough	ADJ
american_scientific_journal-2511	185	17	thrombin	thrombin	NOUN
american_scientific_journal-2511	185	18	is	be	AUX
american_scientific_journal-2511	185	19	added	add	VERB
american_scientific_journal-2511	185	20	to	to	PART
american_scientific_journal-2511	185	21	neutralize	neutralize	VERB
american_scientific_journal-2511	185	22	all	all	PRON
american_scientific_journal-2511	185	23	of	of	ADP
american_scientific_journal-2511	185	24	the	the	DET
american_scientific_journal-2511	185	25	hirudin	hirudin	NOUN
american_scientific_journal-2511	185	26	present	present	NOUN
american_scientific_journal-2511	185	27	,	,	PUNCT
american_scientific_journal-2511	185	28	table	table	NOUN
american_scientific_journal-2511	185	29	1	1	NUM
american_scientific_journal-2511	185	30	[	[	X
american_scientific_journal-2511	185	31	12,32	12,32	NUM
american_scientific_journal-2511	185	32	]	]	X
american_scientific_journal-2511	185	33	.	.	PUNCT
american_scientific_journal-2511	186	1	5	5	X
american_scientific_journal-2511	186	2	.	.	X
american_scientific_journal-2511	186	3	conclusion	conclusion	NOUN
american_scientific_journal-2511	186	4	hirudin	hirudin	NOUN
american_scientific_journal-2511	186	5	was	be	AUX
american_scientific_journal-2511	186	6	successfully	successfully	ADV
american_scientific_journal-2511	186	7	expressed	express	VERB
american_scientific_journal-2511	186	8	from	from	ADP
american_scientific_journal-2511	186	9	h.	h.	PROPN
american_scientific_journal-2511	186	10	orientalis	orientali	NOUN
american_scientific_journal-2511	186	11	cdna	cdna	VERB
american_scientific_journal-2511	186	12	in	in	ADP
american_scientific_journal-2511	186	13	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2511	186	14	)	)	PUNCT
american_scientific_journal-2511	186	15	strain	strain	NOUN
american_scientific_journal-2511	186	16	by	by	ADP
american_scientific_journal-2511	186	17	using	use	VERB
american_scientific_journal-2511	186	18	pet-16b	pet-16b	NUM
american_scientific_journal-2511	186	19	vector	vector	NOUN
american_scientific_journal-2511	186	20	.	.	PUNCT
american_scientific_journal-2511	187	1	elisa	elisa	PROPN
american_scientific_journal-2511	187	2	was	be	AUX
american_scientific_journal-2511	187	3	very	very	ADV
american_scientific_journal-2511	187	4	effective	effective	ADJ
american_scientific_journal-2511	187	5	method	method	NOUN
american_scientific_journal-2511	187	6	to	to	PART
american_scientific_journal-2511	187	7	evaluate	evaluate	VERB
american_scientific_journal-2511	187	8	hirudin	hirudin	NOUN
american_scientific_journal-2511	187	9	concentration	concentration	NOUN
american_scientific_journal-2511	187	10	after	after	ADP
american_scientific_journal-2511	187	11	purification	purification	NOUN
american_scientific_journal-2511	187	12	.	.	PUNCT
american_scientific_journal-2511	188	1	hirudin	hirudin	VERB
american_scientific_journal-2511	188	2	activity	activity	NOUN
american_scientific_journal-2511	188	3	was	be	AUX
american_scientific_journal-2511	188	4	measured	measure	VERB
american_scientific_journal-2511	188	5	by	by	ADP
american_scientific_journal-2511	188	6	thrombin	thrombin	NOUN
american_scientific_journal-2511	188	7	titration	titration	NOUN
american_scientific_journal-2511	188	8	method	method	NOUN
american_scientific_journal-2511	188	9	suggested	suggest	VERB
american_scientific_journal-2511	188	10	about	about	ADP
american_scientific_journal-2511	188	11	360µl	360µl	NOUN
american_scientific_journal-2511	188	12	of	of	ADP
american_scientific_journal-2511	188	13	thrombin	thrombin	NOUN
american_scientific_journal-2511	188	14	required	require	VERB
american_scientific_journal-2511	188	15	for	for	ADP
american_scientific_journal-2511	188	16	make	make	VERB
american_scientific_journal-2511	188	17	clotting	clotting	NOUN
american_scientific_journal-2511	188	18	.	.	PUNCT
american_scientific_journal-2511	189	1	6	6	NUM
american_scientific_journal-2511	189	2	.	.	X
american_scientific_journal-2511	189	3	recommendation	recommendation	NOUN
american_scientific_journal-2511	189	4	analyzing	analyzing	NOUN
american_scientific_journal-2511	189	5	of	of	ADP
american_scientific_journal-2511	189	6	amplified	amplify	VERB
american_scientific_journal-2511	189	7	hirudin	hirudin	VERB
american_scientific_journal-2511	189	8	gene	gene	NOUN
american_scientific_journal-2511	189	9	by	by	ADP
american_scientific_journal-2511	189	10	genetic	genetic	ADJ
american_scientific_journal-2511	189	11	analyzer	analyzer	NOUN
american_scientific_journal-2511	189	12	,	,	PUNCT
american_scientific_journal-2511	189	13	also	also	ADV
american_scientific_journal-2511	189	14	,	,	PUNCT
american_scientific_journal-2511	189	15	amplification	amplification	NOUN
american_scientific_journal-2511	189	16	of	of	ADP
american_scientific_journal-2511	189	17	hirudin	hirudin	VERB
american_scientific_journal-2511	189	18	gene	gene	NOUN
american_scientific_journal-2511	189	19	by	by	ADP
american_scientific_journal-2511	189	20	the	the	DET
american_scientific_journal-2511	189	21	same	same	ADJ
american_scientific_journal-2511	189	22	primers	primer	NOUN
american_scientific_journal-2511	189	23	with	with	ADP
american_scientific_journal-2511	189	24	the	the	DET
american_scientific_journal-2511	189	25	addition	addition	NOUN
american_scientific_journal-2511	189	26	restriction	restriction	NOUN
american_scientific_journal-2511	189	27	enzyme	enzyme	NOUN
american_scientific_journal-2511	189	28	site	site	NOUN
american_scientific_journal-2511	189	29	to	to	ADP
american_scientific_journal-2511	189	30	each	each	DET
american_scientific_journal-2511	189	31	primers	primer	NOUN
american_scientific_journal-2511	189	32	appropriated	appropriate	VERB
american_scientific_journal-2511	189	33	to	to	ADP
american_scientific_journal-2511	189	34	the	the	DET
american_scientific_journal-2511	189	35	pet-16b	pet-16b	NUM
american_scientific_journal-2511	189	36	expression	expression	NOUN
american_scientific_journal-2511	189	37	vector	vector	NOUN
american_scientific_journal-2511	189	38	.	.	PUNCT
american_scientific_journal-2511	190	1	further	further	ADJ
american_scientific_journal-2511	190	2	purification	purification	NOUN
american_scientific_journal-2511	190	3	by	by	ADP
american_scientific_journal-2511	190	4	using	use	VERB
american_scientific_journal-2511	190	5	thrombin	thrombin	PROPN
american_scientific_journal-2511	190	6	sepharose	sepharose	NOUN
american_scientific_journal-2511	190	7	column	column	PROPN
american_scientific_journal-2511	190	8	,	,	PUNCT
american_scientific_journal-2511	190	9	which	which	PRON
american_scientific_journal-2511	190	10	is	be	AUX
american_scientific_journal-2511	190	11	a	a	DET
american_scientific_journal-2511	190	12	best	good	ADJ
american_scientific_journal-2511	190	13	choice	choice	NOUN
american_scientific_journal-2511	190	14	for	for	ADP
american_scientific_journal-2511	190	15	purification	purification	NOUN
american_scientific_journal-2511	190	16	of	of	ADP
american_scientific_journal-2511	190	17	hirudin	hirudin	VERB
american_scientific_journal-2511	190	18	protein	protein	NOUN
american_scientific_journal-2511	190	19	.	.	PUNCT
american_scientific_journal-2511	191	1	acknowledgments	acknowledgment	NOUN
american_scientific_journal-2511	191	2	the	the	DET
american_scientific_journal-2511	191	3	authors	author	NOUN
american_scientific_journal-2511	191	4	are	be	AUX
american_scientific_journal-2511	191	5	grateful	grateful	ADJ
american_scientific_journal-2511	191	6	to	to	ADP
american_scientific_journal-2511	191	7	the	the	DET
american_scientific_journal-2511	191	8	laboratory	laboratory	NOUN
american_scientific_journal-2511	191	9	of	of	ADP
american_scientific_journal-2511	191	10	cell	cell	NOUN
american_scientific_journal-2511	191	11	and	and	CCONJ
american_scientific_journal-2511	191	12	biotechnology	biotechnology	NOUN
american_scientific_journal-2511	191	13	researches	research	VERB
american_scientific_journal-2511	191	14	unit	unit	NOUN
american_scientific_journal-2511	191	15	.	.	PUNCT
american_scientific_journal-2511	192	1	author	author	NOUN
american_scientific_journal-2511	192	2	contributions	contributions	PROPN
american_scientific_journal-2511	192	3	dr	dr	PROPN
american_scientific_journal-2511	192	4	.	.	PROPN
american_scientific_journal-2511	192	5	adnan	adnan	PROPN
american_scientific_journal-2511	193	1	al	al	PROPN
american_scientific_journal-2511	193	2	-	-	PUNCT
american_scientific_journal-2511	193	3	badran	badran	PROPN
american_scientific_journal-2511	193	4	contributed	contribute	VERB
american_scientific_journal-2511	193	5	to	to	ADP
american_scientific_journal-2511	193	6	the	the	DET
american_scientific_journal-2511	193	7	conception	conception	NOUN
american_scientific_journal-2511	193	8	and	and	CCONJ
american_scientific_journal-2511	193	9	design	design	NOUN
american_scientific_journal-2511	193	10	of	of	ADP
american_scientific_journal-2511	193	11	the	the	DET
american_scientific_journal-2511	193	12	study	study	NOUN
american_scientific_journal-2511	193	13	,	,	PUNCT
american_scientific_journal-2511	193	14	sabaa	sabaa	PROPN
american_scientific_journal-2511	193	15	al	al	PROPN
american_scientific_journal-2511	193	16	-	-	PUNCT
american_scientific_journal-2511	193	17	fadal	fadal	PROPN
american_scientific_journal-2511	193	18	contributed	contribute	VERB
american_scientific_journal-2511	193	19	to	to	ADP
american_scientific_journal-2511	193	20	the	the	DET
american_scientific_journal-2511	193	21	conception	conception	NOUN
american_scientific_journal-2511	193	22	and	and	CCONJ
american_scientific_journal-2511	193	23	design	design	NOUN
american_scientific_journal-2511	193	24	of	of	ADP
american_scientific_journal-2511	193	25	the	the	DET
american_scientific_journal-2511	193	26	study	study	NOUN
american_scientific_journal-2511	193	27	,	,	PUNCT
american_scientific_journal-2511	193	28	performed	perform	VERB
american_scientific_journal-2511	193	29	experimental	experimental	ADJ
american_scientific_journal-2511	193	30	work	work	NOUN
american_scientific_journal-2511	193	31	and	and	CCONJ
american_scientific_journal-2511	193	32	participated	participate	VERB
american_scientific_journal-2511	193	33	in	in	ADP
american_scientific_journal-2511	193	34	data	datum	NOUN
american_scientific_journal-2511	193	35	analysis	analysis	NOUN
american_scientific_journal-2511	193	36	.	.	PUNCT
american_scientific_journal-2511	194	1	both	both	DET
american_scientific_journal-2511	194	2	authors	author	NOUN
american_scientific_journal-2511	194	3	american	american	PROPN
american_scientific_journal-2511	194	4	scientific	scientific	ADJ
american_scientific_journal-2511	194	5	research	research	PROPN
american_scientific_journal-2511	194	6	journal	journal	NOUN
american_scientific_journal-2511	194	7	for	for	ADP
american_scientific_journal-2511	194	8	engineering	engineering	NOUN
american_scientific_journal-2511	194	9	,	,	PUNCT
american_scientific_journal-2511	194	10	technology	technology	NOUN
american_scientific_journal-2511	194	11	,	,	PUNCT
american_scientific_journal-2511	194	12	and	and	CCONJ
american_scientific_journal-2511	194	13	sciences	science	NOUN
american_scientific_journal-2511	194	14	(	(	PUNCT
american_scientific_journal-2511	194	15	asrjets	asrjet	NOUN
american_scientific_journal-2511	194	16	)	)	PUNCT
american_scientific_journal-2511	194	17	(	(	PUNCT
american_scientific_journal-2511	194	18	2017	2017	NUM
american_scientific_journal-2511	194	19	)	)	PUNCT
american_scientific_journal-2511	194	20	volume	volume	NOUN
american_scientific_journal-2511	194	21	27	27	NUM
american_scientific_journal-2511	194	22	,	,	PUNCT
american_scientific_journal-2511	194	23	no	no	DET
american_scientific_journal-2511	194	24	1	1	NUM
american_scientific_journal-2511	194	25	,	,	PUNCT
american_scientific_journal-2511	194	26	pp	pp	ADV
american_scientific_journal-2511	194	27	25	25	NUM
american_scientific_journal-2511	194	28	-	-	SYM
american_scientific_journal-2511	194	29	42	42	NUM
american_scientific_journal-2511	194	30	41	41	NUM
american_scientific_journal-2511	194	31	read	read	VERB
american_scientific_journal-2511	194	32	and	and	CCONJ
american_scientific_journal-2511	194	33	approved	approve	VERB
american_scientific_journal-2511	194	34	the	the	DET
american_scientific_journal-2511	194	35	final	final	ADJ
american_scientific_journal-2511	194	36	manuscript	manuscript	NOUN
american_scientific_journal-2511	194	37	.	.	PUNCT
american_scientific_journal-2511	195	1	competing	compete	VERB
american_scientific_journal-2511	195	2	interests	interest	NOUN
american_scientific_journal-2511	195	3	the	the	DET
american_scientific_journal-2511	195	4	authors	author	NOUN
american_scientific_journal-2511	195	5	declare	declare	VERB
american_scientific_journal-2511	195	6	no	no	DET
american_scientific_journal-2511	195	7	conflict	conflict	NOUN
american_scientific_journal-2511	195	8	of	of	ADP
american_scientific_journal-2511	195	9	interest	interest	NOUN
american_scientific_journal-2511	195	10	.	.	PUNCT
american_scientific_journal-2511	196	1	references	reference	NOUN
american_scientific_journal-2511	196	2	[	[	X
american_scientific_journal-2511	196	3	1	1	NUM
american_scientific_journal-2511	196	4	]	]	X
american_scientific_journal-2511	196	5	apakupakul	apakupakul	PROPN
american_scientific_journal-2511	196	6	,	,	PUNCT
american_scientific_journal-2511	196	7	k.	k.	PROPN
american_scientific_journal-2511	196	8	,	,	PUNCT
american_scientific_journal-2511	196	9	siddall	siddall	PROPN
american_scientific_journal-2511	196	10	,	,	PUNCT
american_scientific_journal-2511	196	11	m.	m.	PROPN
american_scientific_journal-2511	196	12	e.	e.	PROPN
american_scientific_journal-2511	196	13	and	and	CCONJ
american_scientific_journal-2511	196	14	burreson	burreson	PROPN
american_scientific_journal-2511	196	15	,	,	PUNCT
american_scientific_journal-2511	196	16	e.	e.	PROPN
american_scientific_journal-2511	196	17	m.	m.	PROPN
american_scientific_journal-2511	196	18	higher	high	ADJ
american_scientific_journal-2511	196	19	level	level	NOUN
american_scientific_journal-2511	196	20	relationships	relationship	NOUN
american_scientific_journal-2511	196	21	of	of	ADP
american_scientific_journal-2511	196	22	leeches	leech	NOUN
american_scientific_journal-2511	196	23	(	(	PUNCT
american_scientific_journal-2511	196	24	annelida	annelida	NOUN
american_scientific_journal-2511	196	25	:	:	PUNCT
american_scientific_journal-2511	196	26	clitellata	clitellata	PROPN
american_scientific_journal-2511	196	27	:	:	PUNCT
american_scientific_journal-2511	196	28	euhirudinea	euhirudinea	PROPN
american_scientific_journal-2511	196	29	)	)	PUNCT
american_scientific_journal-2511	196	30	based	base	VERB
american_scientific_journal-2511	196	31	on	on	ADP
american_scientific_journal-2511	196	32	morphology	morphology	NOUN
american_scientific_journal-2511	196	33	and	and	CCONJ
american_scientific_journal-2511	196	34	gene	gene	NOUN
american_scientific_journal-2511	196	35	sequences	sequence	NOUN
american_scientific_journal-2511	196	36	.	.	PUNCT
american_scientific_journal-2511	197	1	molecular	molecular	ADJ
american_scientific_journal-2511	197	2	phylogenetics	phylogenetic	NOUN
american_scientific_journal-2511	197	3	and	and	CCONJ
american_scientific_journal-2511	197	4	evolution	evolution	NOUN
american_scientific_journal-2511	197	5	,	,	PUNCT
american_scientific_journal-2511	197	6	1999	1999	NUM
american_scientific_journal-2511	197	7	,	,	PUNCT
american_scientific_journal-2511	197	8	12(3):1–10	12(3):1–10	NUM
american_scientific_journal-2511	197	9	.	.	PUNCT
american_scientific_journal-2511	197	10	]	]	PUNCT
american_scientific_journal-2511	198	1	[	[	X
american_scientific_journal-2511	198	2	2	2	NUM
american_scientific_journal-2511	198	3	]	]	PUNCT
american_scientific_journal-2511	198	4	petrauskiene	petrauskiene	NOUN
american_scientific_journal-2511	198	5	,	,	PUNCT
american_scientific_journal-2511	198	6	l.	l.	PROPN
american_scientific_journal-2511	198	7	,	,	PUNCT
american_scientific_journal-2511	198	8	utevska	utevska	PROPN
american_scientific_journal-2511	198	9	,	,	PUNCT
american_scientific_journal-2511	198	10	o.	o.	NOUN
american_scientific_journal-2511	198	11	and	and	CCONJ
american_scientific_journal-2511	198	12	utevsky	utevsky	ADJ
american_scientific_journal-2511	198	13	,	,	PUNCT
american_scientific_journal-2511	198	14	s.	s.	PROPN
american_scientific_journal-2511	198	15	can	can	AUX
american_scientific_journal-2511	198	16	different	different	ADJ
american_scientific_journal-2511	198	17	species	specie	NOUN
american_scientific_journal-2511	198	18	of	of	ADP
american_scientific_journal-2511	198	19	medicinal	medicinal	ADJ
american_scientific_journal-2511	198	20	leeches	leech	NOUN
american_scientific_journal-2511	198	21	(	(	PUNCT
american_scientific_journal-2511	198	22	hirudo	hirudo	NOUN
american_scientific_journal-2511	198	23	spp	spp	NOUN
american_scientific_journal-2511	198	24	.	.	PUNCT
american_scientific_journal-2511	198	25	)	)	PUNCT
american_scientific_journal-2511	198	26	.	.	PUNCT
american_scientific_journal-2511	199	1	interbreed	interbree	VERB
american_scientific_journal-2511	199	2	?	?	PUNCT
american_scientific_journal-2511	199	3	.	.	PUNCT
american_scientific_journal-2511	200	1	invertebrate	invertebrate	NOUN
american_scientific_journal-2511	200	2	biology	biology	NOUN
american_scientific_journal-2511	200	3	,	,	PUNCT
american_scientific_journal-2511	200	4	2009,128(4):324	2009,128(4):324	NUM
american_scientific_journal-2511	200	5	-	-	SYM
american_scientific_journal-2511	200	6	331	331	NUM
american_scientific_journal-2511	201	1	[	[	X
american_scientific_journal-2511	201	2	3	3	NUM
american_scientific_journal-2511	201	3	]	]	X
american_scientific_journal-2511	201	4	abdullah	abdullah	PROPN
american_scientific_journal-2511	201	5	,	,	PUNCT
american_scientific_journal-2511	201	6	s.	s.	PROPN
american_scientific_journal-2511	201	7	,	,	PUNCT
american_scientific_journal-2511	201	8	dar	dar	PROPN
american_scientific_journal-2511	201	9	,	,	PUNCT
american_scientific_journal-2511	201	10	l.m	l.m	PROPN
american_scientific_journal-2511	201	11	.	.	PROPN
american_scientific_journal-2511	201	12	,	,	PUNCT
american_scientific_journal-2511	201	13	rashid	rashid	PROPN
american_scientific_journal-2511	201	14	,	,	PUNCT
american_scientific_journal-2511	201	15	a.	a.	NOUN
american_scientific_journal-2511	201	16	and	and	CCONJ
american_scientific_journal-2511	201	17	tewari	tewari	PROPN
american_scientific_journal-2511	201	18	,	,	PUNCT
american_scientific_journal-2511	201	19	a.	a.	PROPN
american_scientific_journal-2511	201	20	hirudotherapy	hirudotherapy	NOUN
american_scientific_journal-2511	201	21	/	/	SYM
american_scientific_journal-2511	201	22	leech	leech	NOUN
american_scientific_journal-2511	201	23	therapy	therapy	NOUN
american_scientific_journal-2511	201	24	:	:	PUNCT
american_scientific_journal-2511	201	25	application	application	NOUN
american_scientific_journal-2511	201	26	and	and	CCONJ
american_scientific_journal-2511	201	27	indications	indication	NOUN
american_scientific_journal-2511	201	28	in	in	ADP
american_scientific_journal-2511	201	29	surgery	surgery	NOUN
american_scientific_journal-2511	201	30	archives	archive	NOUN
american_scientific_journal-2511	201	31	of	of	ADP
american_scientific_journal-2511	201	32	clinical	clinical	ADJ
american_scientific_journal-2511	201	33	experimental	experimental	ADJ
american_scientific_journal-2511	201	34	surgery	surgery	NOUN
american_scientific_journal-2511	201	35	,	,	PUNCT
american_scientific_journal-2511	201	36	2012,1(3):172	2012,1(3):172	NOUN
american_scientific_journal-2511	201	37	-	-	PUNCT
american_scientific_journal-2511	201	38	180	180	NUM
american_scientific_journal-2511	201	39	[	[	SYM
american_scientific_journal-2511	201	40	4	4	NUM
american_scientific_journal-2511	201	41	]	]	X
american_scientific_journal-2511	201	42	patel	patel	PROPN
american_scientific_journal-2511	201	43	,	,	PUNCT
american_scientific_journal-2511	201	44	r.	r.	PROPN
american_scientific_journal-2511	201	45	k	k	PROPN
american_scientific_journal-2511	201	46	.	.	PUNCT
american_scientific_journal-2511	202	1	hirudin	hirudin	VERB
american_scientific_journal-2511	202	2	-	-	PUNCT
american_scientific_journal-2511	202	3	thrombin	thrombin	NOUN
american_scientific_journal-2511	202	4	complex	complex	NOUN
american_scientific_journal-2511	202	5	:	:	PUNCT
american_scientific_journal-2511	202	6	an	an	DET
american_scientific_journal-2511	202	7	approach	approach	NOUN
american_scientific_journal-2511	202	8	to	to	PART
american_scientific_journal-2511	202	9	prepare	prepare	VERB
american_scientific_journal-2511	202	10	a	a	DET
american_scientific_journal-2511	202	11	blood	blood	NOUN
american_scientific_journal-2511	202	12	compatible	compatible	ADJ
american_scientific_journal-2511	202	13	surface	surface	NOUN
american_scientific_journal-2511	202	14	.	.	PUNCT
american_scientific_journal-2511	203	1	msc	msc	PROPN
american_scientific_journal-2511	203	2	.	.	PROPN
american_scientific_journal-2511	204	1	thesis	thesis	PROPN
american_scientific_journal-2511	204	2	.	.	PUNCT
american_scientific_journal-2511	205	1	department	department	NOUN
american_scientific_journal-2511	205	2	of	of	ADP
american_scientific_journal-2511	205	3	pharmaceutics	pharmaceutic	NOUN
american_scientific_journal-2511	205	4	and	and	CCONJ
american_scientific_journal-2511	205	5	pharmaceutical	pharmaceutical	NOUN
american_scientific_journal-2511	205	6	chemistry	chemistry	NOUN
american_scientific_journal-2511	205	7	.	.	PUNCT
american_scientific_journal-2511	206	1	the	the	DET
american_scientific_journal-2511	206	2	university	university	NOUN
american_scientific_journal-2511	206	3	of	of	ADP
american_scientific_journal-2511	206	4	utah.1995	utah.1995	PROPN
american_scientific_journal-2511	206	5	.	.	PUNCT
american_scientific_journal-2511	207	1	[	[	X
american_scientific_journal-2511	207	2	5	5	NUM
american_scientific_journal-2511	207	3	]	]	PUNCT
american_scientific_journal-2511	207	4	greinacher	greinacher	NOUN
american_scientific_journal-2511	207	5	,	,	PUNCT
american_scientific_journal-2511	207	6	a.	a.	NOUN
american_scientific_journal-2511	207	7	and	and	CCONJ
american_scientific_journal-2511	207	8	warkentin	warkentin	PROPN
american_scientific_journal-2511	207	9	,	,	PUNCT
american_scientific_journal-2511	207	10	t.	t.	PROPN
american_scientific_journal-2511	207	11	e.	e.	PROPN
american_scientific_journal-2511	208	1	the	the	DET
american_scientific_journal-2511	208	2	direct	direct	ADJ
american_scientific_journal-2511	208	3	thrombin	thrombin	NOUN
american_scientific_journal-2511	208	4	inhibitor	inhibitor	NOUN
american_scientific_journal-2511	208	5	hirudin	hirudin	NOUN
american_scientific_journal-2511	208	6	.	.	PUNCT
american_scientific_journal-2511	208	7	thrombosis	thrombosis	NOUN
american_scientific_journal-2511	208	8	and	and	CCONJ
american_scientific_journal-2511	208	9	haemostasis	haemostasis	NOUN
american_scientific_journal-2511	208	10	,	,	PUNCT
american_scientific_journal-2511	208	11	2008	2008	NUM
american_scientific_journal-2511	208	12	,	,	PUNCT
american_scientific_journal-2511	208	13	99(5):819–829	99(5):819–829	NOUN
american_scientific_journal-2511	208	14	.	.	PUNCT
american_scientific_journal-2511	209	1	[	[	X
american_scientific_journal-2511	209	2	6	6	NUM
american_scientific_journal-2511	209	3	]	]	PUNCT
american_scientific_journal-2511	209	4	nisio	nisio	NOUN
american_scientific_journal-2511	209	5	,	,	PUNCT
american_scientific_journal-2511	209	6	m.	m.	PROPN
american_scientific_journal-2511	209	7	d.	d.	PROPN
american_scientific_journal-2511	209	8	,	,	PUNCT
american_scientific_journal-2511	209	9	middeldorp	middeldorp	PROPN
american_scientific_journal-2511	209	10	,	,	PUNCT
american_scientific_journal-2511	209	11	s.	s.	PROPN
american_scientific_journal-2511	209	12	and	and	CCONJ
american_scientific_journal-2511	209	13	buller	buller	PROPN
american_scientific_journal-2511	209	14	,	,	PUNCT
american_scientific_journal-2511	209	15	h.	h.	PROPN
american_scientific_journal-2511	209	16	r.	r.	PROPN
american_scientific_journal-2511	209	17	direct	direct	VERB
american_scientific_journal-2511	209	18	thrombin	thrombin	NOUN
american_scientific_journal-2511	209	19	inhibitors	inhibitor	NOUN
american_scientific_journal-2511	209	20	.	.	PUNCT
american_scientific_journal-2511	210	1	the	the	DET
american_scientific_journal-2511	210	2	new	new	PROPN
american_scientific_journal-2511	210	3	england	england	PROPN
american_scientific_journal-2511	210	4	journal	journal	PROPN
american_scientific_journal-2511	210	5	of	of	ADP
american_scientific_journal-2511	210	6	medicine	medicine	NOUN
american_scientific_journal-2511	210	7	,	,	PUNCT
american_scientific_journal-2511	210	8	2005	2005	NUM
american_scientific_journal-2511	210	9	,	,	PUNCT
american_scientific_journal-2511	210	10	353:1028	353:1028	NUM
american_scientific_journal-2511	210	11	-	-	SYM
american_scientific_journal-2511	210	12	40	40	NUM
american_scientific_journal-2511	211	1	[	[	X
american_scientific_journal-2511	211	2	7	7	NUM
american_scientific_journal-2511	211	3	]	]	SYM
american_scientific_journal-2511	211	4	mille	mille	PROPN
american_scientific_journal-2511	211	5	,	,	PUNCT
american_scientific_journal-2511	211	6	b.	b.	PROPN
american_scientific_journal-2511	211	7	,	,	PUNCT
american_scientific_journal-2511	211	8	condamines	condamine	NOUN
american_scientific_journal-2511	211	9	,	,	PUNCT
american_scientific_journal-2511	211	10	o.	o.	PROPN
american_scientific_journal-2511	211	11	,	,	PUNCT
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american_scientific_journal-2511	211	13	,	,	PUNCT
american_scientific_journal-2511	211	14	j.m	j.m	PROPN
american_scientific_journal-2511	211	15	.	.	PROPN
american_scientific_journal-2511	211	16	,	,	PUNCT
american_scientific_journal-2511	211	17	maftouh	maftouh	NOUN
american_scientific_journal-2511	211	18	,	,	PUNCT
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american_scientific_journal-2511	211	20	,	,	PUNCT
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american_scientific_journal-2511	211	22	,	,	PUNCT
american_scientific_journal-2511	211	23	c.	c.	PROPN
american_scientific_journal-2511	211	24	,	,	PUNCT
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american_scientific_journal-2511	211	26	,	,	PUNCT
american_scientific_journal-2511	211	27	d.	d.	PROPN
american_scientific_journal-2511	211	28	and	and	CCONJ
american_scientific_journal-2511	211	29	pau	pau	PROPN
american_scientific_journal-2511	211	30	,	,	PUNCT
american_scientific_journal-2511	211	31	b.	b.	PROPN
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american_scientific_journal-2511	211	33	-	-	PUNCT
american_scientific_journal-2511	211	34	site	site	NOUN
american_scientific_journal-2511	211	35	immunoassay	immunoassay	NOUN
american_scientific_journal-2511	211	36	of	of	ADP
american_scientific_journal-2511	211	37	recombinant	recombinant	ADJ
american_scientific_journal-2511	211	38	hirudin	hirudin	NOUN
american_scientific_journal-2511	211	39	based	base	VERB
american_scientific_journal-2511	211	40	on	on	ADP
american_scientific_journal-2511	211	41	two	two	NUM
american_scientific_journal-2511	211	42	monoclonal	monoclonal	NOUN
american_scientific_journal-2511	211	43	antibodies.clinical	antibodies.clinical	PROPN
american_scientific_journal-2511	211	44	chemistry,1994	chemistry,1994	PROPN
american_scientific_journal-2511	211	45	,	,	PUNCT
american_scientific_journal-2511	211	46	40(5):734	40(5):734	NUM
american_scientific_journal-2511	211	47	-	-	SYM
american_scientific_journal-2511	211	48	739	739	NUM
american_scientific_journal-2511	212	1	[	[	SYM
american_scientific_journal-2511	212	2	8	8	NUM
american_scientific_journal-2511	212	3	]	]	X
american_scientific_journal-2511	212	4	elliott	elliott	PROPN
american_scientific_journal-2511	212	5	,	,	PUNCT
american_scientific_journal-2511	212	6	j.	j.	PROPN
american_scientific_journal-2511	212	7	and	and	CCONJ
american_scientific_journal-2511	212	8	kutschera	kutschera	PROPN
american_scientific_journal-2511	212	9	,	,	PUNCT
american_scientific_journal-2511	212	10	u.	u.	PROPN
american_scientific_journal-2511	212	11	medicinal	medicinal	ADJ
american_scientific_journal-2511	212	12	leeches	leech	NOUN
american_scientific_journal-2511	212	13	:	:	PUNCT
american_scientific_journal-2511	212	14	historical	historical	ADJ
american_scientific_journal-2511	212	15	use	use	NOUN
american_scientific_journal-2511	212	16	,	,	PUNCT
american_scientific_journal-2511	212	17	ecology	ecology	NOUN
american_scientific_journal-2511	212	18	,	,	PUNCT
american_scientific_journal-2511	212	19	genetics	genetic	NOUN
american_scientific_journal-2511	212	20	and	and	CCONJ
american_scientific_journal-2511	212	21	conservation	conservation	NOUN
american_scientific_journal-2511	212	22	.	.	PUNCT
american_scientific_journal-2511	213	1	freshwater	freshwater	PROPN
american_scientific_journal-2511	213	2	reviews,2011	reviews,2011	PROPN
american_scientific_journal-2511	213	3	,	,	PUNCT
american_scientific_journal-2511	213	4	4(1):21–41	4(1):21–41	NUM
american_scientific_journal-2511	213	5	.	.	PUNCT
american_scientific_journal-2511	214	1	[	[	X
american_scientific_journal-2511	214	2	9	9	NUM
american_scientific_journal-2511	214	3	]	]	PUNCT
american_scientific_journal-2511	214	4	novagen	novagen	NOUN
american_scientific_journal-2511	214	5	.	.	PUNCT
american_scientific_journal-2511	215	1	competent	competent	ADJ
american_scientific_journal-2511	215	2	cells	cell	NOUN
american_scientific_journal-2511	215	3	user	user	NOUN
american_scientific_journal-2511	215	4	manual	manual	NOUN
american_scientific_journal-2511	215	5	.	.	PUNCT
american_scientific_journal-2511	216	1	novagen	novagen	PROPN
american_scientific_journal-2511	216	2	catalog	catalog	PROPN
american_scientific_journal-2511	216	3	.	.	PUNCT
american_scientific_journal-2511	217	1	2011	2011	NUM
american_scientific_journal-2511	218	1	[	[	X
american_scientific_journal-2511	218	2	10	10	NUM
american_scientific_journal-2511	218	3	]	]	X
american_scientific_journal-2511	218	4	sambrook	sambrook	NOUN
american_scientific_journal-2511	218	5	,	,	PUNCT
american_scientific_journal-2511	218	6	j.	j.	PROPN
american_scientific_journal-2511	218	7	,	,	PUNCT
american_scientific_journal-2511	218	8	frtish	frtish	NOUN
american_scientific_journal-2511	218	9	,	,	PUNCT
american_scientific_journal-2511	218	10	e.	e.	PROPN
american_scientific_journal-2511	218	11	and	and	CCONJ
american_scientific_journal-2511	218	12	maniatis	maniatis	PROPN
american_scientific_journal-2511	218	13	,	,	PUNCT
american_scientific_journal-2511	218	14	t.	t.	NOUN
american_scientific_journal-2511	218	15	molecular	molecular	ADJ
american_scientific_journal-2511	218	16	cloning	cloning	NOUN
american_scientific_journal-2511	218	17	,	,	PUNCT
american_scientific_journal-2511	218	18	a	a	DET
american_scientific_journal-2511	218	19	laboratory	laboratory	NOUN
american_scientific_journal-2511	218	20	mannual	mannual	ADJ
american_scientific_journal-2511	218	21	.	.	PUNCT
american_scientific_journal-2511	219	1	2nd	2nd	ADJ
american_scientific_journal-2511	219	2	ed	ed	NOUN
american_scientific_journal-2511	219	3	.	.	PUNCT
american_scientific_journal-2511	220	1	gold	gold	ADJ
american_scientific_journal-2511	220	2	spring	spring	NOUN
american_scientific_journal-2511	220	3	harbor	harbor	NOUN
american_scientific_journal-2511	220	4	laboratory	laboratory	PROPN
american_scientific_journal-2511	220	5	press.usa	press.usa	X
american_scientific_journal-2511	220	6	,	,	PUNCT
american_scientific_journal-2511	220	7	1989	1989	NUM
american_scientific_journal-2511	220	8	[	[	X
american_scientific_journal-2511	220	9	11	11	NUM
american_scientific_journal-2511	220	10	]	]	X
american_scientific_journal-2511	220	11	santos	santos	PROPN
american_scientific_journal-2511	220	12	,	,	PUNCT
american_scientific_journal-2511	220	13	c.a	c.a	PROPN
american_scientific_journal-2511	220	14	.	.	PROPN
american_scientific_journal-2511	220	15	,	,	PUNCT
american_scientific_journal-2511	220	16	beloti	beloti	PROPN
american_scientific_journal-2511	220	17	,	,	PUNCT
american_scientific_journal-2511	220	18	l.l	l.l	PROPN
american_scientific_journal-2511	220	19	.	.	PROPN
american_scientific_journal-2511	220	20	,	,	PUNCT
american_scientific_journal-2511	220	21	toledo	toledo	PROPN
american_scientific_journal-2511	220	22	,	,	PUNCT
american_scientific_journal-2511	220	23	m.a	m.a	PROPN
american_scientific_journal-2511	220	24	.	.	PROPN
american_scientific_journal-2511	220	25	,	,	PUNCT
american_scientific_journal-2511	220	26	crucello	crucello	PROPN
american_scientific_journal-2511	220	27	,	,	PUNCT
american_scientific_journal-2511	220	28	a.	a.	NOUN
american_scientific_journal-2511	220	29	,	,	PUNCT
american_scientific_journal-2511	220	30	favaro	favaro	PROPN
american_scientific_journal-2511	220	31	,	,	PUNCT
american_scientific_journal-2511	220	32	m.t	m.t	PROPN
american_scientific_journal-2511	220	33	.	.	PROPN
american_scientific_journal-2511	220	34	,	,	PUNCT
american_scientific_journal-2511	220	35	mendes	mende	NOUN
american_scientific_journal-2511	220	36	,	,	PUNCT
american_scientific_journal-2511	220	37	j.s	j.s	PROPN
american_scientific_journal-2511	220	38	.	.	PROPN
american_scientific_journal-2511	220	39	,	,	PUNCT
american_scientific_journal-2511	220	40	santiago	santiago	PROPN
american_scientific_journal-2511	220	41	,	,	PUNCT
american_scientific_journal-2511	220	42	a.s	a.s	PROPN
american_scientific_journal-2511	220	43	.	.	PROPN
american_scientific_journal-2511	220	44	,	,	PUNCT
american_scientific_journal-2511	220	45	azoni	azoni	PROPN
american_scientific_journal-2511	220	46	,	,	PUNCT
american_scientific_journal-2511	220	47	a.r	a.r	PROPN
american_scientific_journal-2511	220	48	.	.	PROPN
american_scientific_journal-2511	220	49	and	and	CCONJ
american_scientific_journal-2511	220	50	souza	souza	PROPN
american_scientific_journal-2511	220	51	,	,	PUNCT
american_scientific_journal-2511	220	52	a.p	a.p	PROPN
american_scientific_journal-2511	220	53	.	.	PROPN
american_scientific_journal-2511	220	54	a	a	DET
american_scientific_journal-2511	220	55	novel	novel	ADJ
american_scientific_journal-2511	220	56	protein	protein	NOUN
american_scientific_journal-2511	220	57	refolding	refolde	VERB
american_scientific_journal-2511	220	58	protocol	protocol	NOUN
american_scientific_journal-2511	220	59	for	for	ADP
american_scientific_journal-2511	220	60	the	the	DET
american_scientific_journal-2511	220	61	solubilization	solubilization	NOUN
american_scientific_journal-2511	220	62	and	and	CCONJ
american_scientific_journal-2511	220	63	purification	purification	NOUN
american_scientific_journal-2511	220	64	of	of	ADP
american_scientific_journal-2511	220	65	recombinant	recombinant	ADJ
american_scientific_journal-2511	220	66	peptidoglycan	peptidoglycan	ADJ
american_scientific_journal-2511	220	67	-	-	PUNCT
american_scientific_journal-2511	220	68	associated	associate	VERB
american_scientific_journal-2511	220	69	lipoprotein	lipoprotein	NOUN
american_scientific_journal-2511	220	70	from	from	ADP
american_scientific_journal-2511	220	71	xylella	xylella	PROPN
american_scientific_journal-2511	220	72	fastidiosa	fastidiosa	PROPN
american_scientific_journal-2511	220	73	overexpressed	overexpresse	VERB
american_scientific_journal-2511	220	74	in	in	ADP
american_scientific_journal-2511	220	75	escherichia	escherichia	NOUN
american_scientific_journal-2511	220	76	coli	coli	NOUN
american_scientific_journal-2511	220	77	.	.	PUNCT
american_scientific_journal-2511	221	1	protein	protein	NOUN
american_scientific_journal-2511	221	2	expression	expression	NOUN
american_scientific_journal-2511	221	3	and	and	CCONJ
american_scientific_journal-2511	221	4	purification	purification	NOUN
american_scientific_journal-2511	221	5	,	,	PUNCT
american_scientific_journal-2511	221	6	2012	2012	NUM
american_scientific_journal-2511	221	7	,	,	PUNCT
american_scientific_journal-2511	221	8	82	82	NUM
american_scientific_journal-2511	221	9	:	:	SYM
american_scientific_journal-2511	221	10	284	284	NUM
american_scientific_journal-2511	221	11	-	-	SYM
american_scientific_journal-2511	221	12	289	289	NUM
american_scientific_journal-2511	222	1	[	[	X
american_scientific_journal-2511	222	2	12	12	NUM
american_scientific_journal-2511	222	3	]	]	X
american_scientific_journal-2511	222	4	markwardt	markwardt	ADJ
american_scientific_journal-2511	222	5	,	,	PUNCT
american_scientific_journal-2511	222	6	f.	f.	PROPN
american_scientific_journal-2511	222	7	hirudin	hirudin	NOUN
american_scientific_journal-2511	222	8	as	as	ADP
american_scientific_journal-2511	222	9	an	an	DET
american_scientific_journal-2511	222	10	inhibitor	inhibitor	NOUN
american_scientific_journal-2511	222	11	of	of	ADP
american_scientific_journal-2511	222	12	thrombin	thrombin	NOUN
american_scientific_journal-2511	222	13	.	.	PUNCT
american_scientific_journal-2511	223	1	method	method	PROPN
american_scientific_journal-2511	223	2	enzymolog	enzymolog	PROPN
american_scientific_journal-2511	223	3	,	,	PUNCT
american_scientific_journal-2511	223	4	1970	1970	NUM
american_scientific_journal-2511	223	5	,	,	PUNCT
american_scientific_journal-2511	223	6	69:924	69:924	NUM
american_scientific_journal-2511	223	7	-	-	SYM
american_scientific_journal-2511	223	8	932	932	NUM
american_scientific_journal-2511	224	1	[	[	PUNCT
american_scientific_journal-2511	224	2	13	13	NUM
american_scientific_journal-2511	224	3	]	]	PUNCT
american_scientific_journal-2511	224	4	trontelj	trontelj	NOUN
american_scientific_journal-2511	224	5	,	,	PUNCT
american_scientific_journal-2511	224	6	p.	p.	NOUN
american_scientific_journal-2511	224	7	and	and	CCONJ
american_scientific_journal-2511	224	8	utevsky	utevsky	ADJ
american_scientific_journal-2511	224	9	,	,	PUNCT
american_scientific_journal-2511	224	10	s.y	s.y	PROPN
american_scientific_journal-2511	224	11	.	.	PROPN
american_scientific_journal-2511	224	12	celebrity	celebrity	NOUN
american_scientific_journal-2511	224	13	with	with	ADP
american_scientific_journal-2511	224	14	a	a	DET
american_scientific_journal-2511	224	15	neglected	neglect	VERB
american_scientific_journal-2511	224	16	taxonomy	taxonomy	NOUN
american_scientific_journal-2511	224	17	:	:	PUNCT
american_scientific_journal-2511	224	18	molecular	molecular	ADJ
american_scientific_journal-2511	224	19	systematics	systematic	NOUN
american_scientific_journal-2511	224	20	of	of	ADP
american_scientific_journal-2511	224	21	the	the	DET
american_scientific_journal-2511	224	22	medicinal	medicinal	ADJ
american_scientific_journal-2511	224	23	leech	leech	NOUN
american_scientific_journal-2511	224	24	(	(	PUNCT
american_scientific_journal-2511	224	25	genus	genus	NOUN
american_scientific_journal-2511	224	26	hirudo	hirudo	NOUN
american_scientific_journal-2511	224	27	)	)	PUNCT
american_scientific_journal-2511	224	28	.	.	PUNCT
american_scientific_journal-2511	225	1	molecular	molecular	ADJ
american_scientific_journal-2511	225	2	phylogenetics	phylogenetic	NOUN
american_scientific_journal-2511	225	3	and	and	CCONJ
american_scientific_journal-2511	225	4	evolution	evolution	NOUN
american_scientific_journal-2511	225	5	,	,	PUNCT
american_scientific_journal-2511	225	6	2005,34:616–624	2005,34:616–624	NUM
american_scientific_journal-2511	225	7	.	.	PUNCT
american_scientific_journal-2511	226	1	[	[	X
american_scientific_journal-2511	226	2	14	14	NUM
american_scientific_journal-2511	226	3	]	]	X
american_scientific_journal-2511	226	4	meyer	meyer	PROPN
american_scientific_journal-2511	226	5	,	,	PUNCT
american_scientific_journal-2511	226	6	a.	a.	PROPN
american_scientific_journal-2511	226	7	,	,	PUNCT
american_scientific_journal-2511	226	8	todt	todt	PROPN
american_scientific_journal-2511	226	9	,	,	PUNCT
american_scientific_journal-2511	226	10	c.	c.	PROPN
american_scientific_journal-2511	226	11	,	,	PUNCT
american_scientific_journal-2511	226	12	mikkelsen	mikkelsen	PROPN
american_scientific_journal-2511	226	13	,	,	PUNCT
american_scientific_journal-2511	226	14	n.	n.	NOUN
american_scientific_journal-2511	226	15	t.	t.	PROPN
american_scientific_journal-2511	226	16	and	and	CCONJ
american_scientific_journal-2511	226	17	lieb	lieb	PROPN
american_scientific_journal-2511	226	18	,	,	PUNCT
american_scientific_journal-2511	226	19	b.	b.	PROPN
american_scientific_journal-2511	226	20	fast	fast	ADJ
american_scientific_journal-2511	226	21	evolving	evolve	VERB
american_scientific_journal-2511	226	22	18s	18	NOUN
american_scientific_journal-2511	226	23	rrna	rrna	VERB
american_scientific_journal-2511	226	24	sequences	sequence	NOUN
american_scientific_journal-2511	226	25	from	from	ADP
american_scientific_journal-2511	226	26	solenogastres	solenogastre	NOUN
american_scientific_journal-2511	226	27	(	(	PUNCT
american_scientific_journal-2511	226	28	mollusca	mollusca	NOUN
american_scientific_journal-2511	226	29	)	)	PUNCT
american_scientific_journal-2511	226	30	resist	resist	VERB
american_scientific_journal-2511	226	31	standard	standard	ADJ
american_scientific_journal-2511	226	32	pcr	pcr	ADJ
american_scientific_journal-2511	226	33	amplification	amplification	NOUN
american_scientific_journal-2511	226	34	and	and	CCONJ
american_scientific_journal-2511	226	35	give	give	VERB
american_scientific_journal-2511	226	36	new	new	ADJ
american_scientific_journal-2511	226	37	insights	insight	NOUN
american_scientific_journal-2511	226	38	into	into	ADP
american_scientific_journal-2511	226	39	mollusk	mollusk	NOUN
american_scientific_journal-2511	226	40	substitution	substitution	NOUN
american_scientific_journal-2511	226	41	rate	rate	NOUN
american_scientific_journal-2511	226	42	heterogeneity	heterogeneity	NOUN
american_scientific_journal-2511	226	43	.	.	PUNCT
american_scientific_journal-2511	227	1	biomed	biome	VERB
american_scientific_journal-2511	227	2	central	central	ADJ
american_scientific_journal-2511	227	3	evolutionary	evolutionary	ADJ
american_scientific_journal-2511	227	4	biology	biology	NOUN
american_scientific_journal-2511	227	5	,	,	PUNCT
american_scientific_journal-2511	227	6	2010	2010	NUM
american_scientific_journal-2511	227	7	,	,	PUNCT
american_scientific_journal-2511	227	8	10:70	10:70	NUM
american_scientific_journal-2511	227	9	[	[	X
american_scientific_journal-2511	227	10	15	15	NUM
american_scientific_journal-2511	227	11	]	]	X
american_scientific_journal-2511	227	12	wu	wu	PROPN
american_scientific_journal-2511	227	13	,	,	PUNCT
american_scientific_journal-2511	227	14	s.	s.	PROPN
american_scientific_journal-2511	227	15	,	,	PUNCT
american_scientific_journal-2511	227	16	xiong	xiong	PROPN
american_scientific_journal-2511	227	17	,	,	PUNCT
american_scientific_journal-2511	227	18	j.	j.	PROPN
american_scientific_journal-2511	227	19	and	and	CCONJ
american_scientific_journal-2511	227	20	yu	yu	PROPN
american_scientific_journal-2511	227	21	,	,	PUNCT
american_scientific_journal-2511	227	22	y.	y.	PROPN
american_scientific_journal-2511	227	23	y.	y.	NOUN
american_scientific_journal-2511	227	24	taxonomic	taxonomic	ADJ
american_scientific_journal-2511	227	25	resolutions	resolution	NOUN
american_scientific_journal-2511	227	26	based	base	VERB
american_scientific_journal-2511	227	27	on	on	ADP
american_scientific_journal-2511	227	28	18s	18s	NUM
american_scientific_journal-2511	227	29	rrna	rrna	PROPN
american_scientific_journal-2511	227	30	genes	gene	NOUN
american_scientific_journal-2511	227	31	:	:	PUNCT
american_scientific_journal-2511	227	32	a	a	DET
american_scientific_journal-2511	227	33	case	case	NOUN
american_scientific_journal-2511	227	34	study	study	NOUN
american_scientific_journal-2511	227	35	of	of	ADP
american_scientific_journal-2511	227	36	subclass	subclass	ADJ
american_scientific_journal-2511	227	37	copepoda	copepoda	NOUN
american_scientific_journal-2511	227	38	.	.	PUNCT
american_scientific_journal-2511	228	1	plos	plos	PROPN
american_scientific_journal-2511	228	2	one	one	NUM
american_scientific_journal-2511	228	3	public	public	ADJ
american_scientific_journal-2511	228	4	library	library	NOUN
american_scientific_journal-2511	228	5	of	of	ADP
american_scientific_journal-2511	228	6	science	science	NOUN
american_scientific_journal-2511	228	7	,	,	PUNCT
american_scientific_journal-2511	228	8	2015,10(6):1–19	2015,10(6):1–19	NUM
american_scientific_journal-2511	228	9	american	american	ADJ
american_scientific_journal-2511	228	10	scientific	scientific	ADJ
american_scientific_journal-2511	228	11	research	research	NOUN
american_scientific_journal-2511	228	12	journal	journal	NOUN
american_scientific_journal-2511	228	13	for	for	ADP
american_scientific_journal-2511	228	14	engineering	engineering	NOUN
american_scientific_journal-2511	228	15	,	,	PUNCT
american_scientific_journal-2511	228	16	technology	technology	NOUN
american_scientific_journal-2511	228	17	,	,	PUNCT
american_scientific_journal-2511	228	18	and	and	CCONJ
american_scientific_journal-2511	228	19	sciences	science	NOUN
american_scientific_journal-2511	228	20	(	(	PUNCT
american_scientific_journal-2511	228	21	asrjets	asrjet	NOUN
american_scientific_journal-2511	228	22	)	)	PUNCT
american_scientific_journal-2511	228	23	(	(	PUNCT
american_scientific_journal-2511	228	24	2017	2017	NUM
american_scientific_journal-2511	228	25	)	)	PUNCT
american_scientific_journal-2511	228	26	volume	volume	NOUN
american_scientific_journal-2511	228	27	27	27	NUM
american_scientific_journal-2511	228	28	,	,	PUNCT
american_scientific_journal-2511	228	29	no	no	DET
american_scientific_journal-2511	228	30	1	1	NUM
american_scientific_journal-2511	228	31	,	,	PUNCT
american_scientific_journal-2511	228	32	pp	pp	ADV
american_scientific_journal-2511	228	33	25	25	NUM
american_scientific_journal-2511	228	34	-	-	SYM
american_scientific_journal-2511	228	35	42	42	NUM
american_scientific_journal-2511	228	36	42	42	NUM
american_scientific_journal-2511	229	1	[	[	X
american_scientific_journal-2511	229	2	16	16	NUM
american_scientific_journal-2511	229	3	]	]	X
american_scientific_journal-2511	229	4	hebert	hebert	NOUN
american_scientific_journal-2511	229	5	,	,	PUNCT
american_scientific_journal-2511	229	6	p.d.n	p.d.n	PROPN
american_scientific_journal-2511	229	7	.	.	PROPN
american_scientific_journal-2511	229	8	,	,	PUNCT
american_scientific_journal-2511	229	9	ratnasingham	ratnasingham	PROPN
american_scientific_journal-2511	229	10	,	,	PUNCT
american_scientific_journal-2511	229	11	s.	s.	PROPN
american_scientific_journal-2511	229	12	and	and	CCONJ
american_scientific_journal-2511	229	13	waard	waard	PROPN
american_scientific_journal-2511	229	14	,	,	PUNCT
american_scientific_journal-2511	229	15	j.	j.	PROPN
american_scientific_journal-2511	229	16	barcoding	barcode	VERB
american_scientific_journal-2511	229	17	animal	animal	NOUN
american_scientific_journal-2511	229	18	life	life	NOUN
american_scientific_journal-2511	229	19	 	 	SPACE
american_scientific_journal-2511	229	20	:	:	PUNCT
american_scientific_journal-2511	229	21	cytochrome	cytochrome	PROPN
american_scientific_journal-2511	229	22	c	c	NOUN
american_scientific_journal-2511	229	23	oxidase	oxidase	NOUN
american_scientific_journal-2511	229	24	subunit	subunit	NOUN
american_scientific_journal-2511	229	25	1	1	NUM
american_scientific_journal-2511	229	26	divergences	divergence	NOUN
american_scientific_journal-2511	229	27	among	among	ADP
american_scientific_journal-2511	229	28	closely	closely	ADV
american_scientific_journal-2511	229	29	related	relate	VERB
american_scientific_journal-2511	229	30	species	specie	NOUN
american_scientific_journal-2511	229	31	barcoding	barcode	VERB
american_scientific_journal-2511	229	32	animal	animal	NOUN
american_scientific_journal-2511	229	33	life	life	NOUN
american_scientific_journal-2511	229	34	 	 	SPACE
american_scientific_journal-2511	229	35	:	:	PUNCT
american_scientific_journal-2511	229	36	cytochrome	cytochrome	PROPN
american_scientific_journal-2511	229	37	c	c	NOUN
american_scientific_journal-2511	229	38	oxidase	oxidase	NOUN
american_scientific_journal-2511	229	39	subunit	subunit	NOUN
american_scientific_journal-2511	229	40	1	1	NUM
american_scientific_journal-2511	229	41	divergences	divergence	NOUN
american_scientific_journal-2511	229	42	among	among	ADP
american_scientific_journal-2511	229	43	closely	closely	ADV
american_scientific_journal-2511	229	44	related	relate	VERB
american_scientific_journal-2511	229	45	species	specie	NOUN
american_scientific_journal-2511	229	46	.	.	PUNCT
american_scientific_journal-2511	230	1	proceeding	proceed	VERB
american_scientific_journal-2511	230	2	of	of	ADP
american_scientific_journal-2511	230	3	royal	royal	ADJ
american_scientific_journal-2511	230	4	society	society	NOUN
american_scientific_journal-2511	230	5	of	of	ADP
american_scientific_journal-2511	230	6	london	london	PROPN
american_scientific_journal-2511	230	7	,	,	PUNCT
american_scientific_journal-2511	230	8	2003	2003	NUM
american_scientific_journal-2511	230	9	,	,	PUNCT
american_scientific_journal-2511	230	10	270	270	NUM
american_scientific_journal-2511	230	11	:	:	PUNCT
american_scientific_journal-2511	230	12	s96	s96	NOUN
american_scientific_journal-2511	230	13	–	–	PUNCT
american_scientific_journal-2511	230	14	s99	s99	NOUN
american_scientific_journal-2511	230	15	[	[	X
american_scientific_journal-2511	230	16	17	17	NUM
american_scientific_journal-2511	230	17	]	]	SYM
american_scientific_journal-2511	230	18	laufer	laufer	NOUN
american_scientific_journal-2511	230	19	,	,	PUNCT
american_scientific_journal-2511	230	20	a.	a.	PROPN
american_scientific_journal-2511	230	21	s.	s.	PROPN
american_scientific_journal-2511	230	22	,	,	PUNCT
american_scientific_journal-2511	230	23	siddall	siddall	PROPN
american_scientific_journal-2511	230	24	,	,	PUNCT
american_scientific_journal-2511	230	25	m.	m.	PROPN
american_scientific_journal-2511	230	26	e.	e.	PROPN
american_scientific_journal-2511	230	27	and	and	CCONJ
american_scientific_journal-2511	230	28	graf	graf	PROPN
american_scientific_journal-2511	230	29	,	,	PUNCT
american_scientific_journal-2511	230	30	j.	j.	PROPN
american_scientific_journal-2511	230	31	characterization	characterization	NOUN
american_scientific_journal-2511	230	32	of	of	ADP
american_scientific_journal-2511	230	33	the	the	DET
american_scientific_journal-2511	230	34	digestive	digestive	ADJ
american_scientific_journal-2511	230	35	-	-	PUNCT
american_scientific_journal-2511	230	36	tract	tract	NOUN
american_scientific_journal-2511	230	37	microbiota	microbiota	NOUN
american_scientific_journal-2511	230	38	of	of	ADP
american_scientific_journal-2511	230	39	hirudo	hirudo	NOUN
american_scientific_journal-2511	230	40	orientalis	orientali	NOUN
american_scientific_journal-2511	230	41	,	,	PUNCT
american_scientific_journal-2511	230	42	a	a	DET
american_scientific_journal-2511	230	43	european	european	ADJ
american_scientific_journal-2511	230	44	medicinal	medicinal	ADJ
american_scientific_journal-2511	230	45	leech	leech	NOUN
american_scientific_journal-2511	230	46	.	.	PUNCT
american_scientific_journal-2511	231	1	applied	apply	VERB
american_scientific_journal-2511	231	2	and	and	CCONJ
american_scientific_journal-2511	231	3	environmental	environmental	ADJ
american_scientific_journal-2511	231	4	microbiology	microbiology	NOUN
american_scientific_journal-2511	231	5	,	,	PUNCT
american_scientific_journal-2511	231	6	2008	2008	NUM
american_scientific_journal-2511	231	7	,	,	PUNCT
american_scientific_journal-2511	231	8	74(19	74(19	NUM
american_scientific_journal-2511	231	9	):	):	PUNCT
american_scientific_journal-2511	231	10	6151	6151	NUM
american_scientific_journal-2511	231	11	–	–	PUNCT
american_scientific_journal-2511	231	12	6154	6154	NUM
american_scientific_journal-2511	231	13	.	.	PUNCT
american_scientific_journal-2511	232	1	[	[	X
american_scientific_journal-2511	232	2	18	18	NUM
american_scientific_journal-2511	232	3	]	]	X
american_scientific_journal-2511	232	4	qiagen	qiagen	PROPN
american_scientific_journal-2511	232	5	.	.	PUNCT
american_scientific_journal-2511	233	1	rneasy	rneasy	PROPN
american_scientific_journal-2511	233	2	®	®	PROPN
american_scientific_journal-2511	233	3	plus	plus	CCONJ
american_scientific_journal-2511	233	4	mini	mini	ADJ
american_scientific_journal-2511	233	5	handbook	handbook	NOUN
american_scientific_journal-2511	233	6	.	.	PUNCT
american_scientific_journal-2511	234	1	2014	2014	NUM
american_scientific_journal-2511	235	1	[	[	X
american_scientific_journal-2511	235	2	19	19	NUM
american_scientific_journal-2511	235	3	]	]	X
american_scientific_journal-2511	235	4	padmanabhan	padmanabhan	PROPN
american_scientific_journal-2511	235	5	,	,	PUNCT
american_scientific_journal-2511	235	6	s.	s.	PROPN
american_scientific_journal-2511	235	7	,	,	PUNCT
american_scientific_journal-2511	235	8	banerjee	banerjee	PROPN
american_scientific_journal-2511	235	9	,	,	PUNCT
american_scientific_journal-2511	235	10	s.	s.	PROPN
american_scientific_journal-2511	235	11	and	and	CCONJ
american_scientific_journal-2511	235	12	mandi	mandi	PROPN
american_scientific_journal-2511	235	13	,	,	PUNCT
american_scientific_journal-2511	235	14	n.	n.	PROPN
american_scientific_journal-2511	235	15	screening	screening	NOUN
american_scientific_journal-2511	235	16	of	of	ADP
american_scientific_journal-2511	235	17	bacterial	bacterial	ADJ
american_scientific_journal-2511	235	18	recombinants	recombinant	NOUN
american_scientific_journal-2511	235	19	:	:	PUNCT
american_scientific_journal-2511	235	20	strategies	strategy	NOUN
american_scientific_journal-2511	235	21	and	and	CCONJ
american_scientific_journal-2511	235	22	preventing	prevent	VERB
american_scientific_journal-2511	235	23	false	false	ADJ
american_scientific_journal-2511	235	24	positive	positive	ADJ
american_scientific_journal-2511	235	25	.	.	PUNCT
american_scientific_journal-2511	236	1	molecular	molecular	ADJ
american_scientific_journal-2511	236	2	cloning	cloning	NOUN
american_scientific_journal-2511	236	3	–	–	PUNCT
american_scientific_journal-2511	236	4	selected	select	VERB
american_scientific_journal-2511	236	5	applications	application	NOUN
american_scientific_journal-2511	236	6	in	in	ADP
american_scientific_journal-2511	236	7	medicine	medicine	NOUN
american_scientific_journal-2511	236	8	and	and	CCONJ
american_scientific_journal-2511	236	9	biology,2011,pp1	biology,2011,pp1	PROPN
american_scientific_journal-2511	236	10	-	-	PUNCT
american_scientific_journal-2511	236	11	19	19	NUM
american_scientific_journal-2511	236	12	.	.	PUNCT
american_scientific_journal-2511	236	13	avaliable	avaliable	ADJ
american_scientific_journal-2511	236	14	at	at	ADP
american_scientific_journal-2511	236	15	http://www.intechopen.com/books/molecular-cloning-selectedapplications-in-medicine-and-biology/screening-of-bacterial-recombinants-strategies-and-preventingfalse-positives	http://www.intechopen.com/books/molecular-cloning-selectedapplications-in-medicine-and-biology/screening-of-bacterial-recombinants-strategies-and-preventingfalse-positive	NOUN
american_scientific_journal-2511	236	16	[	[	X
american_scientific_journal-2511	236	17	20	20	NUM
american_scientific_journal-2511	236	18	]	]	X
american_scientific_journal-2511	236	19	joseph	joseph	PROPN
american_scientific_journal-2511	236	20	,	,	PUNCT
american_scientific_journal-2511	236	21	b.c	b.c	PROPN
american_scientific_journal-2511	236	22	.	.	PROPN
american_scientific_journal-2511	236	23	,	,	PUNCT
american_scientific_journal-2511	236	24	pichiamuthu	pichiamuthu	PROPN
american_scientific_journal-2511	236	25	,	,	PUNCT
american_scientific_journal-2511	236	26	s.	s.	PROPN
american_scientific_journal-2511	236	27	,	,	PUNCT
american_scientific_journal-2511	236	28	srimeenakshi	srimeenakshi	PROPN
american_scientific_journal-2511	236	29	,	,	PUNCT
american_scientific_journal-2511	236	30	s.	s.	PROPN
american_scientific_journal-2511	236	31	,	,	PUNCT
american_scientific_journal-2511	236	32	murthy	murthy	PROPN
american_scientific_journal-2511	236	33	,	,	PUNCT
american_scientific_journal-2511	236	34	m.	m.	NOUN
american_scientific_journal-2511	236	35	,	,	PUNCT
american_scientific_journal-2511	236	36	selvakumar	selvakumar	PROPN
american_scientific_journal-2511	236	37	,	,	PUNCT
american_scientific_journal-2511	236	38	k.	k.	PROPN
american_scientific_journal-2511	236	39	,	,	PUNCT
american_scientific_journal-2511	236	40	ganesan	ganesan	PROPN
american_scientific_journal-2511	236	41	,	,	PUNCT
american_scientific_journal-2511	236	42	m.	m.	NOUN
american_scientific_journal-2511	236	43	and	and	CCONJ
american_scientific_journal-2511	236	44	manjunath	manjunath	PROPN
american_scientific_journal-2511	236	45	,	,	PUNCT
american_scientific_journal-2511	236	46	s.	s.	PROPN
american_scientific_journal-2511	236	47	r.	r.	PROPN
american_scientific_journal-2511	236	48	an	an	DET
american_scientific_journal-2511	236	49	overview	overview	NOUN
american_scientific_journal-2511	236	50	of	of	ADP
american_scientific_journal-2511	236	51	the	the	DET
american_scientific_journal-2511	236	52	parameters	parameter	NOUN
american_scientific_journal-2511	236	53	for	for	ADP
american_scientific_journal-2511	236	54	recombinant	recombinant	ADJ
american_scientific_journal-2511	236	55	protein	protein	NOUN
american_scientific_journal-2511	236	56	expression	expression	NOUN
american_scientific_journal-2511	236	57	in	in	ADP
american_scientific_journal-2511	236	58	escherichia	escherichia	NOUN
american_scientific_journal-2511	236	59	coli	coli	NOUN
american_scientific_journal-2511	236	60	.	.	PUNCT
american_scientific_journal-2511	237	1	cell	cell	NOUN
american_scientific_journal-2511	237	2	science	science	NOUN
american_scientific_journal-2511	237	3	and	and	CCONJ
american_scientific_journal-2511	237	4	therapy	therapy	NOUN
american_scientific_journal-2511	237	5	,	,	PUNCT
american_scientific_journal-2511	237	6	2015	2015	NUM
american_scientific_journal-2511	237	7	,	,	PUNCT
american_scientific_journal-2511	237	8	6(5	6(5	NUM
american_scientific_journal-2511	237	9	):	):	PUNCT
american_scientific_journal-2511	237	10	pp.1–7	pp.1–7	PROPN
american_scientific_journal-2511	238	1	[	[	X
american_scientific_journal-2511	238	2	21	21	NUM
american_scientific_journal-2511	238	3	]	]	X
american_scientific_journal-2511	238	4	promega	promega	PROPN
american_scientific_journal-2511	238	5	.	.	PUNCT
american_scientific_journal-2511	239	1	subcloning	subclone	VERB
american_scientific_journal-2511	239	2	notebook	notebook	NOUN
american_scientific_journal-2511	239	3	.	.	PUNCT
american_scientific_journal-2511	240	1	promega	promega	PROPN
american_scientific_journal-2511	240	2	corporation	corporation	PROPN
american_scientific_journal-2511	240	3	.	.	PUNCT
american_scientific_journal-2511	241	1	2004	2004	NUM
american_scientific_journal-2511	242	1	[	[	X
american_scientific_journal-2511	242	2	22	22	NUM
american_scientific_journal-2511	242	3	]	]	PUNCT
american_scientific_journal-2511	242	4	ginzinger	ginzinger	NOUN
american_scientific_journal-2511	242	5	,	,	PUNCT
american_scientific_journal-2511	242	6	d.	d.	PROPN
american_scientific_journal-2511	242	7	g.	g.	PROPN
american_scientific_journal-2511	242	8	gene	gene	NOUN
american_scientific_journal-2511	242	9	quantification	quantification	NOUN
american_scientific_journal-2511	242	10	using	use	VERB
american_scientific_journal-2511	242	11	real	real	ADJ
american_scientific_journal-2511	242	12	-	-	PUNCT
american_scientific_journal-2511	242	13	time	time	NOUN
american_scientific_journal-2511	242	14	quantitative	quantitative	ADJ
american_scientific_journal-2511	242	15	pcr	pcr	NOUN
american_scientific_journal-2511	242	16	 	 	SPACE
american_scientific_journal-2511	242	17	:	:	PUNCT
american_scientific_journal-2511	242	18	an	an	DET
american_scientific_journal-2511	242	19	emerging	emerge	VERB
american_scientific_journal-2511	242	20	technology	technology	NOUN
american_scientific_journal-2511	242	21	hits	hit	VERB
american_scientific_journal-2511	242	22	the	the	DET
american_scientific_journal-2511	242	23	mainstream.experimental	mainstream.experimental	PROPN
american_scientific_journal-2511	242	24	hematology	hematology	NOUN
american_scientific_journal-2511	242	25	,	,	PUNCT
american_scientific_journal-2511	242	26	2002	2002	NUM
american_scientific_journal-2511	242	27	,	,	PUNCT
american_scientific_journal-2511	242	28	30:503–512	30:503–512	PROPN
american_scientific_journal-2511	242	29	.	.	PUNCT
american_scientific_journal-2511	243	1	[	[	X
american_scientific_journal-2511	243	2	23	23	NUM
american_scientific_journal-2511	243	3	]	]	X
american_scientific_journal-2511	243	4	fraga	fraga	NOUN
american_scientific_journal-2511	243	5	,	,	PUNCT
american_scientific_journal-2511	243	6	d.	d.	PROPN
american_scientific_journal-2511	243	7	,	,	PUNCT
american_scientific_journal-2511	243	8	meulia	meulia	PROPN
american_scientific_journal-2511	243	9	,	,	PUNCT
american_scientific_journal-2511	243	10	t.	t.	NOUN
american_scientific_journal-2511	243	11	and	and	CCONJ
american_scientific_journal-2511	243	12	fenster	fenster	PROPN
american_scientific_journal-2511	243	13	,	,	PUNCT
american_scientific_journal-2511	243	14	s.	s.	PROPN
american_scientific_journal-2511	243	15	real	real	ADJ
american_scientific_journal-2511	243	16	-	-	PUNCT
american_scientific_journal-2511	243	17	time	time	NOUN
american_scientific_journal-2511	243	18	pcr	pcr	NOUN
american_scientific_journal-2511	243	19	.	.	PUNCT
american_scientific_journal-2511	244	1	current	current	ADJ
american_scientific_journal-2511	244	2	protocols	protocol	NOUN
american_scientific_journal-2511	244	3	essential	essential	ADJ
american_scientific_journal-2511	244	4	laboratory	laboratory	NOUN
american_scientific_journal-2511	244	5	techniques	technique	NOUN
american_scientific_journal-2511	244	6	,	,	PUNCT
american_scientific_journal-2511	244	7	2014,64(2):10.3.1	2014,64(2):10.3.1	PROPN
american_scientific_journal-2511	244	8	-	-	SYM
american_scientific_journal-2511	244	9	10.3.40	10.3.40	NUM
american_scientific_journal-2511	244	10	.	.	PUNCT
american_scientific_journal-2511	245	1	[	[	X
american_scientific_journal-2511	245	2	24	24	NUM
american_scientific_journal-2511	245	3	]	]	PUNCT
american_scientific_journal-2511	245	4	life	life	NOUN
american_scientific_journal-2511	245	5	technologies	technology	NOUN
american_scientific_journal-2511	245	6	.	.	PUNCT
american_scientific_journal-2511	246	1	life	life	NOUN
american_scientific_journal-2511	246	2	technologiesreal	technologiesreal	ADJ
american_scientific_journal-2511	246	3	-	-	PUNCT
american_scientific_journal-2511	246	4	time	time	NOUN
american_scientific_journal-2511	246	5	pcr	pcr	PROPN
american_scientific_journal-2511	246	6	handbook	handbook	NOUN
american_scientific_journal-2511	246	7	.	.	PUNCT
american_scientific_journal-2511	247	1	available	available	ADJ
american_scientific_journal-2511	247	2	at	at	ADP
american_scientific_journal-2511	247	3	:	:	PUNCT
american_scientific_journal-2511	247	4	http://find.lifetechnologies.com/	http://find.lifetechnologies.com/	NOUN
american_scientific_journal-2511	247	5	global	global	ADJ
american_scientific_journal-2511	247	6	/filelib	/filelib	PROPN
american_scientific_journal-2511	247	7	/	/	SYM
american_scientific_journal-2511	247	8	qpcr/	qpcr/	NUM
american_scientific_journal-2511	247	9	real	real	ADJ
american_scientific_journal-2511	247	10	.	.	PUNCT
american_scientific_journal-2511	248	1	2012	2012	NUM
american_scientific_journal-2511	248	2	[	[	X
american_scientific_journal-2511	248	3	25	25	NUM
american_scientific_journal-2511	248	4	]	]	X
american_scientific_journal-2511	248	5	bornhorst	bornhorst	PROPN
american_scientific_journal-2511	248	6	,	,	PUNCT
american_scientific_journal-2511	248	7	j.	j.	PROPN
american_scientific_journal-2511	248	8	a.	a.	PROPN
american_scientific_journal-2511	248	9	and	and	CCONJ
american_scientific_journal-2511	248	10	falke	falke	PROPN
american_scientific_journal-2511	248	11	,	,	PUNCT
american_scientific_journal-2511	248	12	j.	j.	PROPN
american_scientific_journal-2511	248	13	j.	j.	PROPN
american_scientific_journal-2511	248	14	purification	purification	PROPN
american_scientific_journal-2511	248	15	of	of	ADP
american_scientific_journal-2511	248	16	proteins	protein	NOUN
american_scientific_journal-2511	248	17	using	use	VERB
american_scientific_journal-2511	248	18	polyhistidine	polyhistidine	NOUN
american_scientific_journal-2511	248	19	affinity	affinity	NOUN
american_scientific_journal-2511	248	20	tags	tag	NOUN
american_scientific_journal-2511	248	21	.	.	PUNCT
american_scientific_journal-2511	249	1	methods	method	NOUN
american_scientific_journal-2511	249	2	in	in	ADP
american_scientific_journal-2511	249	3	enzymology	enzymology	NOUN
american_scientific_journal-2511	249	4	,	,	PUNCT
american_scientific_journal-2511	249	5	2002	2002	NUM
american_scientific_journal-2511	249	6	,	,	PUNCT
american_scientific_journal-2511	249	7	(	(	PUNCT
american_scientific_journal-2511	249	8	326	326	NUM
american_scientific_journal-2511	249	9	):	):	PUNCT
american_scientific_journal-2511	249	10	245–254	245–254	NUM
american_scientific_journal-2511	249	11	.	.	PUNCT
american_scientific_journal-2511	250	1	[	[	AUX
american_scientific_journal-2511	250	2	26	26	NUM
american_scientific_journal-2511	250	3	]	]	X
american_scientific_journal-2511	250	4	promega	promega	PROPN
american_scientific_journal-2511	250	5	.	.	PUNCT
american_scientific_journal-2511	250	6	hislink	hislink	VERB
american_scientific_journal-2511	250	7	™	™	ADJ
american_scientific_journal-2511	250	8	protein	protein	NOUN
american_scientific_journal-2511	250	9	purification	purification	NOUN
american_scientific_journal-2511	250	10	resin	resin	NOUN
american_scientific_journal-2511	250	11	.	.	PUNCT
american_scientific_journal-2511	251	1	promeg	promeg	PROPN
american_scientific_journal-2511	251	2	catalog	catalog	PROPN
american_scientific_journal-2511	251	3	.	.	PUNCT
american_scientific_journal-2511	252	1	promega	promega	PROPN
american_scientific_journal-2511	252	2	corporatio	corporatio	PROPN
american_scientific_journal-2511	252	3	.	.	PUNCT
american_scientific_journal-2511	253	1	2013	2013	NUM
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american_scientific_journal-2511	254	2	27	27	NUM
american_scientific_journal-2511	254	3	]	]	PUNCT
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american_scientific_journal-2511	254	5	,	,	PUNCT
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american_scientific_journal-2511	254	21	.	.	PUNCT
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american_scientific_journal-2511	255	4	,	,	PUNCT
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american_scientific_journal-2511	256	2	28	28	NUM
american_scientific_journal-2511	256	3	]	]	X
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american_scientific_journal-2511	256	5	,	,	PUNCT
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american_scientific_journal-2511	256	23	:	:	PUNCT
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american_scientific_journal-2511	256	33	.	.	PUNCT
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american_scientific_journal-2511	257	3	:	:	PUNCT
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american_scientific_journal-2511	258	5	,	,	PUNCT
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american_scientific_journal-2511	258	14	.	.	PUNCT
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american_scientific_journal-2511	260	5	,	,	PUNCT
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american_scientific_journal-2511	260	8	,	,	PUNCT
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american_scientific_journal-2511	260	10	,	,	PUNCT
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american_scientific_journal-2511	265	5	,	,	PUNCT
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american_scientific_journal-2511	265	10	,	,	PUNCT
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american_scientific_journal-2511	265	13	,	,	PUNCT
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american_scientific_journal-2511	265	15	,	,	PUNCT
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american_scientific_journal-2511	265	17	,	,	PUNCT
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american_scientific_journal-2511	267	3	http://www.intechopen.com/books/molecular-cloning-selected-applications-in-medicine-and-biology/screening-of-bacterial-recombinants-strategies-and-preventing-false-positives	http://www.intechopen.com/books/molecular-cloning-selected-applications-in-medicine-and-biology/screening-of-bacterial-recombinants-strategies-and-preventing-false-positive	NOUN