id	sid	tid	token	lemma	pos
american_scientific_journal-2520	1	1	12	12	NUM
american_scientific_journal-2520	1	2	american	american	ADJ
american_scientific_journal-2520	1	3	scientific	scientific	ADJ
american_scientific_journal-2520	1	4	research	research	NOUN
american_scientific_journal-2520	1	5	journal	journal	NOUN
american_scientific_journal-2520	1	6	for	for	ADP
american_scientific_journal-2520	1	7	engineering	engineering	NOUN
american_scientific_journal-2520	1	8	,	,	PUNCT
american_scientific_journal-2520	1	9	technology	technology	NOUN
american_scientific_journal-2520	1	10	,	,	PUNCT
american_scientific_journal-2520	1	11	and	and	CCONJ
american_scientific_journal-2520	1	12	sciences	science	NOUN
american_scientific_journal-2520	1	13	(	(	PUNCT
american_scientific_journal-2520	1	14	asrjets	asrjet	NOUN
american_scientific_journal-2520	1	15	)	)	PUNCT
american_scientific_journal-2520	1	16	issn	issn	PROPN
american_scientific_journal-2520	1	17	(	(	PUNCT
american_scientific_journal-2520	1	18	print	print	NOUN
american_scientific_journal-2520	1	19	)	)	PUNCT
american_scientific_journal-2520	1	20	2313	2313	NUM
american_scientific_journal-2520	1	21	-	-	SYM
american_scientific_journal-2520	1	22	4410	4410	NUM
american_scientific_journal-2520	1	23	,	,	PUNCT
american_scientific_journal-2520	1	24	issn	issn	PROPN
american_scientific_journal-2520	1	25	(	(	PUNCT
american_scientific_journal-2520	1	26	online	online	ADJ
american_scientific_journal-2520	1	27	)	)	PUNCT
american_scientific_journal-2520	1	28	2313	2313	NUM
american_scientific_journal-2520	1	29	-	-	SYM
american_scientific_journal-2520	1	30	4402	4402	NUM
american_scientific_journal-2520	1	31	©	©	PROPN
american_scientific_journal-2520	1	32	global	global	ADJ
american_scientific_journal-2520	1	33	society	society	NOUN
american_scientific_journal-2520	1	34	of	of	ADP
american_scientific_journal-2520	1	35	scientific	scientific	ADJ
american_scientific_journal-2520	1	36	research	research	NOUN
american_scientific_journal-2520	1	37	and	and	CCONJ
american_scientific_journal-2520	1	38	researchers	researcher	NOUN
american_scientific_journal-2520	1	39	http://asrjetsjournal.org/	http://asrjetsjournal.org/	NOUN
american_scientific_journal-2520	1	40	construction	construction	NOUN
american_scientific_journal-2520	1	41	of	of	ADP
american_scientific_journal-2520	1	42	synthetic	synthetic	ADJ
american_scientific_journal-2520	1	43	intact	intact	ADJ
american_scientific_journal-2520	1	44	human	human	ADJ
american_scientific_journal-2520	1	45	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	1	46	hormone	hormone	NOUN
american_scientific_journal-2520	1	47	gene	gene	NOUN
american_scientific_journal-2520	1	48	and	and	CCONJ
american_scientific_journal-2520	1	49	testing	test	VERB
american_scientific_journal-2520	1	50	the	the	DET
american_scientific_journal-2520	1	51	transformation	transformation	NOUN
american_scientific_journal-2520	1	52	efficiency	efficiency	NOUN
american_scientific_journal-2520	1	53	and	and	CCONJ
american_scientific_journal-2520	1	54	expression	expression	NOUN
american_scientific_journal-2520	1	55	of	of	ADP
american_scientific_journal-2520	1	56	it	it	PRON
american_scientific_journal-2520	1	57	in	in	ADP
american_scientific_journal-2520	1	58	e.	e.	PROPN
american_scientific_journal-2520	1	59	coli	coli	PROPN
american_scientific_journal-2520	1	60	strains	strain	VERB
american_scientific_journal-2520	1	61	adnan	adnan	PROPN
american_scientific_journal-2520	1	62	al	al	PROPN
american_scientific_journal-2520	1	63	-	-	PUNCT
american_scientific_journal-2520	1	64	badrana	badrana	PROPN
american_scientific_journal-2520	1	65	,	,	PUNCT
american_scientific_journal-2520	1	66	rafeef	rafeef	ADJ
american_scientific_journal-2520	1	67	a.	a.	NOUN
american_scientific_journal-2520	1	68	abdul	abdul	PROPN
american_scientific_journal-2520	1	69	-	-	PUNCT
american_scientific_journal-2520	1	70	jabbarb	jabbarb	PROPN
american_scientific_journal-2520	1	71	*	*	PROPN
american_scientific_journal-2520	1	72	auniversity	auniversity	NOUN
american_scientific_journal-2520	1	73	of	of	ADP
american_scientific_journal-2520	1	74	basra	basra	PROPN
american_scientific_journal-2520	1	75	,	,	PUNCT
american_scientific_journal-2520	1	76	college	college	NOUN
american_scientific_journal-2520	1	77	of	of	ADP
american_scientific_journal-2520	1	78	science	science	NOUN
american_scientific_journal-2520	1	79	,	,	PUNCT
american_scientific_journal-2520	1	80	department	department	NOUN
american_scientific_journal-2520	1	81	of	of	ADP
american_scientific_journal-2520	1	82	biology	biology	NOUN
american_scientific_journal-2520	1	83	,	,	PUNCT
american_scientific_journal-2520	1	84	basra	basra	PROPN
american_scientific_journal-2520	1	85	,	,	PUNCT
american_scientific_journal-2520	1	86	iraq	iraq	PROPN
american_scientific_journal-2520	1	87	buniversity	buniversity	NOUN
american_scientific_journal-2520	1	88	of	of	ADP
american_scientific_journal-2520	1	89	basra	basra	PROPN
american_scientific_journal-2520	1	90	,	,	PUNCT
american_scientific_journal-2520	1	91	college	college	NOUN
american_scientific_journal-2520	1	92	of	of	ADP
american_scientific_journal-2520	1	93	pharmacy	pharmacy	NOUN
american_scientific_journal-2520	1	94	,	,	PUNCT
american_scientific_journal-2520	1	95	department	department	NOUN
american_scientific_journal-2520	1	96	of	of	ADP
american_scientific_journal-2520	1	97	clinical	clinical	ADJ
american_scientific_journal-2520	1	98	laboratory	laboratory	NOUN
american_scientific_journal-2520	1	99	science	science	NOUN
american_scientific_journal-2520	1	100	,	,	PUNCT
american_scientific_journal-2520	1	101	basra	basra	PROPN
american_scientific_journal-2520	1	102	,	,	PUNCT
american_scientific_journal-2520	1	103	iraq	iraq	PROPN
american_scientific_journal-2520	1	104	bemail	bemail	NOUN
american_scientific_journal-2520	1	105	:	:	PUNCT
american_scientific_journal-2520	2	1	rafeefamir@gmail.com	rafeefamir@gmail.com	X
american_scientific_journal-2520	3	1	abstract	abstract	ADJ
american_scientific_journal-2520	3	2	human	human	ADJ
american_scientific_journal-2520	3	3	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	3	4	hormone	hormone	NOUN
american_scientific_journal-2520	3	5	gene	gene	NOUN
american_scientific_journal-2520	3	6	(	(	PUNCT
american_scientific_journal-2520	3	7	hpth	hpth	NOUN
american_scientific_journal-2520	3	8	gene	gene	NOUN
american_scientific_journal-2520	3	9	)	)	PUNCT
american_scientific_journal-2520	3	10	is	be	AUX
american_scientific_journal-2520	3	11	responsible	responsible	ADJ
american_scientific_journal-2520	3	12	for	for	ADP
american_scientific_journal-2520	3	13	preproparathyroid	preproparathyroid	ADJ
american_scientific_journal-2520	3	14	hormone	hormone	NOUN
american_scientific_journal-2520	3	15	production	production	NOUN
american_scientific_journal-2520	3	16	(	(	PUNCT
american_scientific_journal-2520	3	17	biologically	biologically	ADV
american_scientific_journal-2520	3	18	inactive	inactive	ADJ
american_scientific_journal-2520	3	19	hormone	hormone	NOUN
american_scientific_journal-2520	3	20	)	)	PUNCT
american_scientific_journal-2520	3	21	which	which	PRON
american_scientific_journal-2520	3	22	further	far	ADV
american_scientific_journal-2520	3	23	modified	modify	VERB
american_scientific_journal-2520	3	24	to	to	PART
american_scientific_journal-2520	3	25	form	form	VERB
american_scientific_journal-2520	3	26	the	the	DET
american_scientific_journal-2520	3	27	biologically	biologically	ADV
american_scientific_journal-2520	3	28	active	active	ADJ
american_scientific_journal-2520	3	29	hormone	hormone	NOUN
american_scientific_journal-2520	3	30	,	,	PUNCT
american_scientific_journal-2520	3	31	a	a	DET
american_scientific_journal-2520	3	32	synthetic	synthetic	ADJ
american_scientific_journal-2520	3	33	hpth	hpth	NOUN
american_scientific_journal-2520	3	34	gene	gene	NOUN
american_scientific_journal-2520	3	35	with	with	ADP
american_scientific_journal-2520	3	36	289bp	289bp	NOUN
american_scientific_journal-2520	3	37	which	which	DET
american_scientific_journal-2520	3	38	cod	cod	NOUN
american_scientific_journal-2520	3	39	for	for	ADP
american_scientific_journal-2520	3	40	active	active	ADJ
american_scientific_journal-2520	3	41	intact	intact	ADJ
american_scientific_journal-2520	3	42	hpth	hpth	NOUN
american_scientific_journal-2520	3	43	was	be	AUX
american_scientific_journal-2520	3	44	constructed	construct	VERB
american_scientific_journal-2520	3	45	by	by	ADP
american_scientific_journal-2520	3	46	five	five	NUM
american_scientific_journal-2520	3	47	long	long	ADJ
american_scientific_journal-2520	3	48	primers	primer	NOUN
american_scientific_journal-2520	3	49	by	by	ADP
american_scientific_journal-2520	3	50	splicing	splicing	NOUN
american_scientific_journal-2520	3	51	overlap	overlap	NOUN
american_scientific_journal-2520	3	52	extension	extension	NOUN
american_scientific_journal-2520	3	53	pcr	pcr	NOUN
american_scientific_journal-2520	3	54	(	(	PUNCT
american_scientific_journal-2520	3	55	soe	soe	PROPN
american_scientific_journal-2520	3	56	)	)	PUNCT
american_scientific_journal-2520	3	57	,	,	PUNCT
american_scientific_journal-2520	3	58	the	the	DET
american_scientific_journal-2520	3	59	gene	gene	NOUN
american_scientific_journal-2520	3	60	contain	contain	VERB
american_scientific_journal-2520	3	61	a	a	DET
american_scientific_journal-2520	3	62	de	de	X
american_scientific_journal-2520	3	63	generated	generate	VERB
american_scientific_journal-2520	3	64	codon	codon	NOUN
american_scientific_journal-2520	3	65	,	,	PUNCT
american_scientific_journal-2520	3	66	and	and	CCONJ
american_scientific_journal-2520	3	67	have	have	VERB
american_scientific_journal-2520	3	68	two	two	NUM
american_scientific_journal-2520	3	69	recognition	recognition	NOUN
american_scientific_journal-2520	3	70	site	site	NOUN
american_scientific_journal-2520	3	71	of	of	ADP
american_scientific_journal-2520	3	72	restriction	restriction	NOUN
american_scientific_journal-2520	3	73	enzymes	enzyme	NOUN
american_scientific_journal-2520	3	74	in	in	ADP
american_scientific_journal-2520	3	75	both	both	DET
american_scientific_journal-2520	3	76	ends	end	NOUN
american_scientific_journal-2520	3	77	of	of	ADP
american_scientific_journal-2520	3	78	the	the	DET
american_scientific_journal-2520	3	79	gene	gene	NOUN
american_scientific_journal-2520	3	80	,	,	PUNCT
american_scientific_journal-2520	3	81	and	and	CCONJ
american_scientific_journal-2520	3	82	a	a	DET
american_scientific_journal-2520	3	83	stop	stop	NOUN
american_scientific_journal-2520	3	84	codon	codon	NOUN
american_scientific_journal-2520	3	85	.	.	PUNCT
american_scientific_journal-2520	4	1	the	the	DET
american_scientific_journal-2520	4	2	synthetic	synthetic	ADJ
american_scientific_journal-2520	4	3	hpth	hpth	NOUN
american_scientific_journal-2520	4	4	gene	gene	NOUN
american_scientific_journal-2520	4	5	was	be	AUX
american_scientific_journal-2520	4	6	transformed	transform	VERB
american_scientific_journal-2520	4	7	in	in	ADP
american_scientific_journal-2520	4	8	two	two	NUM
american_scientific_journal-2520	4	9	e.	e.	PROPN
american_scientific_journal-2520	4	10	coli	coli	NOUN
american_scientific_journal-2520	4	11	strains	strain	VERB
american_scientific_journal-2520	4	12	dh5α	dh5α	PROPN
american_scientific_journal-2520	4	13	and	and	CCONJ
american_scientific_journal-2520	5	1	bl21	bl21	PROPN
american_scientific_journal-2520	5	2	(	(	PUNCT
american_scientific_journal-2520	5	3	de3	de3	PROPN
american_scientific_journal-2520	5	4	)	)	PUNCT
american_scientific_journal-2520	5	5	with	with	ADP
american_scientific_journal-2520	5	6	transformation	transformation	NOUN
american_scientific_journal-2520	5	7	efficiency6.6	efficiency6.6	PROPN
american_scientific_journal-2520	5	8	*	*	PROPN
american_scientific_journal-2520	5	9	106	106	NUM
american_scientific_journal-2520	5	10	and	and	CCONJ
american_scientific_journal-2520	5	11	3.4	3.4	NUM
american_scientific_journal-2520	5	12	*	*	SYM
american_scientific_journal-2520	5	13	106	106	NUM
american_scientific_journal-2520	5	14	for	for	ADP
american_scientific_journal-2520	5	15	both	both	DET
american_scientific_journal-2520	5	16	isolates	isolate	NOUN
american_scientific_journal-2520	5	17	respectively	respectively	ADV
american_scientific_journal-2520	5	18	.	.	PUNCT
american_scientific_journal-2520	6	1	and	and	CCONJ
american_scientific_journal-2520	6	2	the	the	DET
american_scientific_journal-2520	6	3	transformation	transformation	NOUN
american_scientific_journal-2520	6	4	of	of	ADP
american_scientific_journal-2520	6	5	gene	gene	NOUN
american_scientific_journal-2520	6	6	was	be	AUX
american_scientific_journal-2520	6	7	detected	detect	VERB
american_scientific_journal-2520	6	8	by	by	ADP
american_scientific_journal-2520	6	9	extraction	extraction	NOUN
american_scientific_journal-2520	6	10	of	of	ADP
american_scientific_journal-2520	6	11	constructed	construct	VERB
american_scientific_journal-2520	6	12	plasmid	plasmid	NOUN
american_scientific_journal-2520	6	13	and	and	CCONJ
american_scientific_journal-2520	6	14	amplification	amplification	NOUN
american_scientific_journal-2520	6	15	of	of	ADP
american_scientific_journal-2520	6	16	cloning	cloning	NOUN
american_scientific_journal-2520	6	17	gene	gene	NOUN
american_scientific_journal-2520	6	18	on	on	ADP
american_scientific_journal-2520	6	19	it	it	PRON
american_scientific_journal-2520	6	20	,	,	PUNCT
american_scientific_journal-2520	6	21	the	the	DET
american_scientific_journal-2520	6	22	expression	expression	NOUN
american_scientific_journal-2520	6	23	of	of	ADP
american_scientific_journal-2520	6	24	the	the	DET
american_scientific_journal-2520	6	25	cloning	cloning	NOUN
american_scientific_journal-2520	6	26	gene	gene	NOUN
american_scientific_journal-2520	6	27	in	in	ADP
american_scientific_journal-2520	6	28	the	the	DET
american_scientific_journal-2520	6	29	bl21	bl21	PROPN
american_scientific_journal-2520	6	30	(	(	PUNCT
american_scientific_journal-2520	6	31	de3	de3	PROPN
american_scientific_journal-2520	6	32	)	)	PUNCT
american_scientific_journal-2520	6	33	was	be	AUX
american_scientific_journal-2520	6	34	detected	detect	VERB
american_scientific_journal-2520	6	35	by	by	ADP
american_scientific_journal-2520	6	36	performing	perform	VERB
american_scientific_journal-2520	6	37	real	real	ADJ
american_scientific_journal-2520	6	38	time	time	NOUN
american_scientific_journal-2520	6	39	pcr	pcr	NOUN
american_scientific_journal-2520	6	40	which	which	PRON
american_scientific_journal-2520	6	41	gave	give	VERB
american_scientific_journal-2520	6	42	a	a	DET
american_scientific_journal-2520	6	43	ct	ct	NUM
american_scientific_journal-2520	6	44	value	value	NOUN
american_scientific_journal-2520	6	45	of	of	ADP
american_scientific_journal-2520	6	46	about	about	ADP
american_scientific_journal-2520	6	47	(	(	PUNCT
american_scientific_journal-2520	6	48	23.46	23.46	NUM
american_scientific_journal-2520	6	49	)	)	PUNCT
american_scientific_journal-2520	6	50	.	.	PUNCT
american_scientific_journal-2520	7	1	that	that	PRON
american_scientific_journal-2520	7	2	makes	make	VERB
american_scientific_journal-2520	7	3	the	the	DET
american_scientific_journal-2520	7	4	synthetic	synthetic	ADJ
american_scientific_journal-2520	7	5	gene	gene	NOUN
american_scientific_journal-2520	7	6	suitable	suitable	ADJ
american_scientific_journal-2520	7	7	for	for	ADP
american_scientific_journal-2520	7	8	direct	direct	ADJ
american_scientific_journal-2520	7	9	expression	expression	NOUN
american_scientific_journal-2520	7	10	of	of	ADP
american_scientific_journal-2520	7	11	human	human	ADJ
american_scientific_journal-2520	7	12	active	active	ADJ
american_scientific_journal-2520	7	13	protein	protein	NOUN
american_scientific_journal-2520	7	14	inside	inside	ADP
american_scientific_journal-2520	7	15	the	the	DET
american_scientific_journal-2520	7	16	bacterial	bacterial	ADJ
american_scientific_journal-2520	7	17	cells	cell	NOUN
american_scientific_journal-2520	7	18	.	.	PUNCT
american_scientific_journal-2520	8	1	keywords	keyword	NOUN
american_scientific_journal-2520	8	2	:	:	PUNCT
american_scientific_journal-2520	8	3	synthetic	synthetic	ADJ
american_scientific_journal-2520	8	4	gene	gene	NOUN
american_scientific_journal-2520	8	5	;	;	PUNCT
american_scientific_journal-2520	8	6	synthetic	synthetic	ADJ
american_scientific_journal-2520	8	7	human	human	ADJ
american_scientific_journal-2520	8	8	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	8	9	hormone	hormone	NOUN
american_scientific_journal-2520	8	10	gene	gene	NOUN
american_scientific_journal-2520	8	11	;	;	PUNCT
american_scientific_journal-2520	8	12	soe	soe	PROPN
american_scientific_journal-2520	8	13	pcr	pcr	PROPN
american_scientific_journal-2520	8	14	.	.	PUNCT
american_scientific_journal-2520	9	1	1	1	X
american_scientific_journal-2520	9	2	.	.	X
american_scientific_journal-2520	9	3	introduction	introduction	NOUN
american_scientific_journal-2520	9	4	human	human	ADJ
american_scientific_journal-2520	9	5	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	9	6	hormone	hormone	NOUN
american_scientific_journal-2520	9	7	normal	normal	ADJ
american_scientific_journal-2520	9	8	gene	gene	NOUN
american_scientific_journal-2520	9	9	is	be	AUX
american_scientific_journal-2520	9	10	located	locate	VERB
american_scientific_journal-2520	9	11	in	in	ADP
american_scientific_journal-2520	9	12	the	the	DET
american_scientific_journal-2520	9	13	short	short	ADJ
american_scientific_journal-2520	9	14	(	(	PUNCT
american_scientific_journal-2520	9	15	p	p	NOUN
american_scientific_journal-2520	9	16	)	)	PUNCT
american_scientific_journal-2520	9	17	arm	arm	NOUN
american_scientific_journal-2520	9	18	between	between	ADP
american_scientific_journal-2520	9	19	positions	position	NOUN
american_scientific_journal-2520	9	20	15.3	15.3	NUM
american_scientific_journal-2520	9	21	and	and	CCONJ
american_scientific_journal-2520	9	22	15.1	15.1	NUM
american_scientific_journal-2520	9	23	on	on	ADP
american_scientific_journal-2520	9	24	chromosome	chromosome	NOUN
american_scientific_journal-2520	9	25	11	11	NUM
american_scientific_journal-2520	10	1	[	[	X
american_scientific_journal-2520	10	2	1	1	NUM
american_scientific_journal-2520	10	3	]	]	PUNCT
american_scientific_journal-2520	10	4	.	.	PUNCT
american_scientific_journal-2520	11	1	-----------------------------------------------------------------------	-----------------------------------------------------------------------	PUNCT
american_scientific_journal-2520	12	1	*	*	PUNCT
american_scientific_journal-2520	12	2	corresponding	correspond	VERB
american_scientific_journal-2520	12	3	author	author	NOUN
american_scientific_journal-2520	12	4	.	.	PUNCT
american_scientific_journal-2520	13	1	http://asrjetsjournal.org/	http://asrjetsjournal.org/	PROPN
american_scientific_journal-2520	13	2	american	american	PROPN
american_scientific_journal-2520	13	3	scientific	scientific	PROPN
american_scientific_journal-2520	13	4	research	research	NOUN
american_scientific_journal-2520	13	5	journal	journal	NOUN
american_scientific_journal-2520	13	6	for	for	ADP
american_scientific_journal-2520	13	7	engineering	engineering	NOUN
american_scientific_journal-2520	13	8	,	,	PUNCT
american_scientific_journal-2520	13	9	technology	technology	NOUN
american_scientific_journal-2520	13	10	,	,	PUNCT
american_scientific_journal-2520	13	11	and	and	CCONJ
american_scientific_journal-2520	13	12	sciences	science	NOUN
american_scientific_journal-2520	13	13	(	(	PUNCT
american_scientific_journal-2520	13	14	asrjets	asrjet	NOUN
american_scientific_journal-2520	13	15	)	)	PUNCT
american_scientific_journal-2520	13	16	(	(	PUNCT
american_scientific_journal-2520	13	17	2017	2017	NUM
american_scientific_journal-2520	13	18	)	)	PUNCT
american_scientific_journal-2520	13	19	volume	volume	NOUN
american_scientific_journal-2520	13	20	27	27	NUM
american_scientific_journal-2520	13	21	,	,	PUNCT
american_scientific_journal-2520	13	22	no	no	DET
american_scientific_journal-2520	13	23	1	1	NUM
american_scientific_journal-2520	13	24	,	,	PUNCT
american_scientific_journal-2520	13	25	pp	pp	ADV
american_scientific_journal-2520	13	26	12	12	NUM
american_scientific_journal-2520	13	27	-	-	SYM
american_scientific_journal-2520	13	28	24	24	NUM
american_scientific_journal-2520	13	29	13	13	NUM
american_scientific_journal-2520	13	30	its	its	PRON
american_scientific_journal-2520	13	31	consists	consist	NOUN
american_scientific_journal-2520	13	32	of	of	ADP
american_scientific_journal-2520	13	33	3	3	NUM
american_scientific_journal-2520	13	34	exons	exon	NOUN
american_scientific_journal-2520	13	35	[	[	X
american_scientific_journal-2520	13	36	2	2	NUM
american_scientific_journal-2520	13	37	]	]	PUNCT
american_scientific_journal-2520	13	38	exon	exon	NOUN
american_scientific_journal-2520	13	39	1	1	NUM
american_scientific_journal-2520	13	40	is	be	AUX
american_scientific_journal-2520	13	41	untranslated	untranslated	ADJ
american_scientific_journal-2520	13	42	,	,	PUNCT
american_scientific_journal-2520	13	43	exon	exon	NOUN
american_scientific_journal-2520	13	44	2	2	NUM
american_scientific_journal-2520	13	45	encodes	encode	NOUN
american_scientific_journal-2520	13	46	a	a	DET
american_scientific_journal-2520	13	47	25	25	NUM
american_scientific_journal-2520	13	48	-	-	PUNCT
american_scientific_journal-2520	13	49	amino	amino	NOUN
american_scientific_journal-2520	13	50	acid	acid	NOUN
american_scientific_journal-2520	13	51	signal	signal	NOUN
american_scientific_journal-2520	13	52	peptide	peptide	NOUN
american_scientific_journal-2520	13	53	which	which	PRON
american_scientific_journal-2520	13	54	is	be	AUX
american_scientific_journal-2520	13	55	a	a	DET
american_scientific_journal-2520	13	56	part	part	NOUN
american_scientific_journal-2520	13	57	of	of	ADP
american_scientific_journal-2520	13	58	the	the	DET
american_scientific_journal-2520	13	59	prohormone	prohormone	NOUN
american_scientific_journal-2520	13	60	,	,	PUNCT
american_scientific_journal-2520	13	61	and	and	CCONJ
american_scientific_journal-2520	13	62	exon	exon	NOUN
american_scientific_journal-2520	13	63	3	3	NUM
american_scientific_journal-2520	13	64	encodes	encode	NOUN
american_scientific_journal-2520	13	65	the	the	DET
american_scientific_journal-2520	13	66	remaining	remain	VERB
american_scientific_journal-2520	13	67	part	part	NOUN
american_scientific_journal-2520	13	68	of	of	ADP
american_scientific_journal-2520	13	69	the	the	DET
american_scientific_journal-2520	13	70	prohormone	prohormone	NOUN
american_scientific_journal-2520	13	71	(	(	PUNCT
american_scientific_journal-2520	13	72	6	6	NUM
american_scientific_journal-2520	13	73	amino	amino	NOUN
american_scientific_journal-2520	13	74	acids	acid	NOUN
american_scientific_journal-2520	13	75	)	)	PUNCT
american_scientific_journal-2520	13	76	and	and	CCONJ
american_scientific_journal-2520	13	77	the	the	DET
american_scientific_journal-2520	13	78	whole	whole	ADJ
american_scientific_journal-2520	13	79	pth	pth	NOUN
american_scientific_journal-2520	13	80	molecule	molecule	NOUN
american_scientific_journal-2520	13	81	(	(	PUNCT
american_scientific_journal-2520	13	82	84	84	NUM
american_scientific_journal-2520	13	83	amino	amino	ADJ
american_scientific_journal-2520	13	84	acids	acid	NOUN
american_scientific_journal-2520	13	85	)	)	PUNCT
american_scientific_journal-2520	13	86	,	,	PUNCT
american_scientific_journal-2520	13	87	this	this	DET
american_scientific_journal-2520	13	88	gene	gene	NOUN
american_scientific_journal-2520	13	89	when	when	SCONJ
american_scientific_journal-2520	13	90	transcribed	transcribe	VERB
american_scientific_journal-2520	13	91	to	to	ADP
american_scientific_journal-2520	13	92	mrna	mrna	PROPN
american_scientific_journal-2520	13	93	and	and	CCONJ
american_scientific_journal-2520	13	94	the	the	DET
american_scientific_journal-2520	13	95	latter	latter	ADJ
american_scientific_journal-2520	13	96	translated	translate	VERB
american_scientific_journal-2520	13	97	a	a	DET
american_scientific_journal-2520	13	98	larger	large	ADJ
american_scientific_journal-2520	13	99	polypeptide	polypeptide	ADJ
american_scientific_journal-2520	13	100	precursor	precursor	NOUN
american_scientific_journal-2520	13	101	called	call	VERB
american_scientific_journal-2520	13	102	pre	pre	ADJ
american_scientific_journal-2520	13	103	-	-	ADJ
american_scientific_journal-2520	13	104	proparathyroid	proparathyroid	ADJ
american_scientific_journal-2520	13	105	hormone	hormone	NOUN
american_scientific_journal-2520	13	106	(	(	PUNCT
american_scientific_journal-2520	13	107	pre	pre	ADJ
american_scientific_journal-2520	13	108	-	-	ADJ
american_scientific_journal-2520	13	109	propth	propth	ADJ
american_scientific_journal-2520	13	110	)	)	PUNCT
american_scientific_journal-2520	13	111	which	which	PRON
american_scientific_journal-2520	13	112	is	be	AUX
american_scientific_journal-2520	13	113	biologically	biologically	ADV
american_scientific_journal-2520	13	114	in	in	ADP
american_scientific_journal-2520	13	115	active	active	ADJ
american_scientific_journal-2520	13	116	containing	contain	VERB
american_scientific_journal-2520	13	117	115	115	NUM
american_scientific_journal-2520	13	118	amino	amino	ADJ
american_scientific_journal-2520	13	119	acids	acid	NOUN
american_scientific_journal-2520	13	120	it	it	PRON
american_scientific_journal-2520	13	121	was	be	AUX
american_scientific_journal-2520	13	122	also	also	ADV
american_scientific_journal-2520	13	123	identified	identify	VERB
american_scientific_journal-2520	13	124	as	as	ADP
american_scientific_journal-2520	13	125	an	an	DET
american_scientific_journal-2520	13	126	early	early	ADJ
american_scientific_journal-2520	13	127	biosynthetic	biosynthetic	ADJ
american_scientific_journal-2520	13	128	product	product	NOUN
american_scientific_journal-2520	13	129	during	during	ADP
american_scientific_journal-2520	13	130	a	a	DET
american_scientific_journal-2520	13	131	studies	study	NOUN
american_scientific_journal-2520	13	132	using	use	VERB
american_scientific_journal-2520	13	133	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	13	134	gland	gland	NOUN
american_scientific_journal-2520	13	135	slices	slice	NOUN
american_scientific_journal-2520	13	136	the	the	DET
american_scientific_journal-2520	13	137	prepropth	prepropth	NOUN
american_scientific_journal-2520	13	138	undergoes	undergo	VERB
american_scientific_journal-2520	13	139	two	two	NUM
american_scientific_journal-2520	13	140	successive	successive	ADJ
american_scientific_journal-2520	13	141	proteolytic	proteolytic	ADJ
american_scientific_journal-2520	13	142	cleavages	cleavage	NOUN
american_scientific_journal-2520	13	143	inside	inside	ADP
american_scientific_journal-2520	13	144	the	the	DET
american_scientific_journal-2520	13	145	produced	produce	VERB
american_scientific_journal-2520	13	146	cell	cell	NOUN
american_scientific_journal-2520	13	147	to	to	PART
american_scientific_journal-2520	13	148	yield	yield	VERB
american_scientific_journal-2520	13	149	active	active	ADJ
american_scientific_journal-2520	13	150	pth	pth	NOUN
american_scientific_journal-2520	13	151	with	with	ADP
american_scientific_journal-2520	13	152	84	84	NUM
american_scientific_journal-2520	13	153	amino	amino	NOUN
american_scientific_journal-2520	13	154	acid	acid	NOUN
american_scientific_journal-2520	13	155	.	.	PUNCT
american_scientific_journal-2520	14	1	and	and	CCONJ
american_scientific_journal-2520	14	2	because	because	SCONJ
american_scientific_journal-2520	14	3	the	the	DET
american_scientific_journal-2520	14	4	using	using	NOUN
american_scientific_journal-2520	14	5	of	of	ADP
american_scientific_journal-2520	14	6	the	the	DET
american_scientific_journal-2520	14	7	cdna	cdna	PROPN
american_scientific_journal-2520	14	8	derived	derive	VERB
american_scientific_journal-2520	14	9	from	from	ADP
american_scientific_journal-2520	14	10	mrna	mrna	PROPN
american_scientific_journal-2520	14	11	for	for	ADP
american_scientific_journal-2520	14	12	active	active	ADJ
american_scientific_journal-2520	14	13	hpth	hpth	NOUN
american_scientific_journal-2520	14	14	production	production	NOUN
american_scientific_journal-2520	14	15	by	by	ADP
american_scientific_journal-2520	14	16	recombinant	recombinant	ADJ
american_scientific_journal-2520	14	17	dna	dna	PROPN
american_scientific_journal-2520	14	18	technology	technology	NOUN
american_scientific_journal-2520	14	19	require	require	VERB
american_scientific_journal-2520	14	20	post	post	ADJ
american_scientific_journal-2520	14	21	translational	translational	ADJ
american_scientific_journal-2520	14	22	modification	modification	NOUN
american_scientific_journal-2520	14	23	as	as	SCONJ
american_scientific_journal-2520	14	24	described	describe	VERB
american_scientific_journal-2520	14	25	above	above	ADV
american_scientific_journal-2520	14	26	,	,	PUNCT
american_scientific_journal-2520	14	27	that	that	PRON
american_scientific_journal-2520	14	28	make	make	VERB
american_scientific_journal-2520	14	29	its	its	PRON
american_scientific_journal-2520	14	30	cloning	cloning	NOUN
american_scientific_journal-2520	14	31	in	in	ADP
american_scientific_journal-2520	14	32	bacterial	bacterial	ADJ
american_scientific_journal-2520	14	33	system	system	NOUN
american_scientific_journal-2520	14	34	not	not	PART
american_scientific_journal-2520	14	35	useful	useful	ADJ
american_scientific_journal-2520	14	36	because	because	SCONJ
american_scientific_journal-2520	14	37	the	the	DET
american_scientific_journal-2520	14	38	latter	latter	ADJ
american_scientific_journal-2520	14	39	are	be	AUX
american_scientific_journal-2520	14	40	deficient	deficient	ADJ
american_scientific_journal-2520	14	41	to	to	PART
american_scientific_journal-2520	14	42	post	post	VERB
american_scientific_journal-2520	14	43	translational	translational	ADJ
american_scientific_journal-2520	14	44	modification	modification	NOUN
american_scientific_journal-2520	14	45	as	as	ADP
american_scientific_journal-2520	14	46	in	in	ADP
american_scientific_journal-2520	14	47	higher	high	ADJ
american_scientific_journal-2520	14	48	organism	organism	NOUN
american_scientific_journal-2520	15	1	[	[	X
american_scientific_journal-2520	15	2	3].therefore	3].therefore	NUM
american_scientific_journal-2520	15	3	for	for	ADP
american_scientific_journal-2520	15	4	expression	expression	NOUN
american_scientific_journal-2520	15	5	of	of	ADP
american_scientific_journal-2520	15	6	this	this	DET
american_scientific_journal-2520	15	7	hormone	hormone	NOUN
american_scientific_journal-2520	15	8	in	in	ADP
american_scientific_journal-2520	15	9	active	active	ADJ
american_scientific_journal-2520	15	10	form	form	NOUN
american_scientific_journal-2520	15	11	in	in	ADP
american_scientific_journal-2520	15	12	bacteria	bacteria	NOUN
american_scientific_journal-2520	15	13	required	require	VERB
american_scientific_journal-2520	15	14	the	the	DET
american_scientific_journal-2520	15	15	using	using	NOUN
american_scientific_journal-2520	15	16	of	of	ADP
american_scientific_journal-2520	15	17	synthetic	synthetic	ADJ
american_scientific_journal-2520	15	18	pth	pth	NOUN
american_scientific_journal-2520	15	19	gene	gene	NOUN
american_scientific_journal-2520	15	20	,	,	PUNCT
american_scientific_journal-2520	15	21	generally	generally	ADV
american_scientific_journal-2520	15	22	synthetic	synthetic	ADJ
american_scientific_journal-2520	15	23	genes	gene	NOUN
american_scientific_journal-2520	15	24	are	be	AUX
american_scientific_journal-2520	15	25	open	open	ADJ
american_scientific_journal-2520	15	26	reading	reading	NOUN
american_scientific_journal-2520	15	27	frame	frame	NOUN
american_scientific_journal-2520	15	28	sequences	sequence	NOUN
american_scientific_journal-2520	15	29	from	from	ADP
american_scientific_journal-2520	15	30	publicly	publicly	ADV
american_scientific_journal-2520	15	31	marked	mark	VERB
american_scientific_journal-2520	15	32	genes	gene	NOUN
american_scientific_journal-2520	15	33	these	these	DET
american_scientific_journal-2520	15	34	sequence	sequence	NOUN
american_scientific_journal-2520	15	35	generated	generate	VERB
american_scientific_journal-2520	15	36	synthetically	synthetically	ADV
american_scientific_journal-2520	15	37	the	the	DET
american_scientific_journal-2520	15	38	synthetic	synthetic	ADJ
american_scientific_journal-2520	15	39	gene	gene	NOUN
american_scientific_journal-2520	15	40	is	be	AUX
american_scientific_journal-2520	15	41	the	the	DET
american_scientific_journal-2520	15	42	product	product	NOUN
american_scientific_journal-2520	15	43	of	of	ADP
american_scientific_journal-2520	15	44	choice	choice	NOUN
american_scientific_journal-2520	15	45	if	if	SCONJ
american_scientific_journal-2520	15	46	no	no	DET
american_scientific_journal-2520	15	47	natural	natural	ADJ
american_scientific_journal-2520	15	48	clone	clone	NOUN
american_scientific_journal-2520	15	49	is	be	AUX
american_scientific_journal-2520	15	50	available	available	ADJ
american_scientific_journal-2520	15	51	or	or	CCONJ
american_scientific_journal-2520	15	52	if	if	SCONJ
american_scientific_journal-2520	15	53	available	available	ADJ
american_scientific_journal-2520	15	54	clones	clone	NOUN
american_scientific_journal-2520	15	55	do	do	AUX
american_scientific_journal-2520	15	56	not	not	PART
american_scientific_journal-2520	15	57	accomplish	accomplish	VERB
american_scientific_journal-2520	15	58	the	the	DET
american_scientific_journal-2520	15	59	specific	specific	ADJ
american_scientific_journal-2520	15	60	requirements	requirement	NOUN
american_scientific_journal-2520	15	61	,	,	PUNCT
american_scientific_journal-2520	15	62	like	like	ADP
american_scientific_journal-2520	15	63	full	full	ADJ
american_scientific_journal-2520	15	64	sequence	sequence	NOUN
american_scientific_journal-2520	15	65	identity	identity	NOUN
american_scientific_journal-2520	15	66	to	to	ADP
american_scientific_journal-2520	15	67	a	a	DET
american_scientific_journal-2520	15	68	database	database	NOUN
american_scientific_journal-2520	15	69	sequence	sequence	NOUN
american_scientific_journal-2520	15	70	.	.	PUNCT
american_scientific_journal-2520	16	1	or	or	CCONJ
american_scientific_journal-2520	16	2	for	for	ADP
american_scientific_journal-2520	16	3	obtaining	obtain	VERB
american_scientific_journal-2520	16	4	sequences	sequence	NOUN
american_scientific_journal-2520	16	5	verified	verify	VERB
american_scientific_journal-2520	16	6	-	-	PUNCT
american_scientific_journal-2520	16	7	cloned	clone	VERB
american_scientific_journal-2520	16	8	dna	dna	NOUN
american_scientific_journal-2520	16	9	,	,	PUNCT
american_scientific_journal-2520	16	10	especially	especially	ADV
american_scientific_journal-2520	16	11	when	when	SCONJ
american_scientific_journal-2520	16	12	the	the	DET
american_scientific_journal-2520	16	13	source	source	NOUN
american_scientific_journal-2520	16	14	of	of	ADP
american_scientific_journal-2520	16	15	biological	biological	ADJ
american_scientific_journal-2520	16	16	material	material	NOUN
american_scientific_journal-2520	16	17	is	be	AUX
american_scientific_journal-2520	16	18	not	not	PART
american_scientific_journal-2520	16	19	readily	readily	ADV
american_scientific_journal-2520	16	20	available	available	ADJ
american_scientific_journal-2520	16	21	(	(	PUNCT
american_scientific_journal-2520	16	22	rare	rare	ADJ
american_scientific_journal-2520	16	23	,	,	PUNCT
american_scientific_journal-2520	16	24	lost	lost	ADJ
american_scientific_journal-2520	16	25	or	or	CCONJ
american_scientific_journal-2520	16	26	dangerous	dangerous	ADJ
american_scientific_journal-2520	16	27	specimens	specimen	NOUN
american_scientific_journal-2520	16	28	)	)	PUNCT
american_scientific_journal-2520	16	29	and	and	CCONJ
american_scientific_journal-2520	16	30	when	when	SCONJ
american_scientific_journal-2520	16	31	required	require	VERB
american_scientific_journal-2520	16	32	dna	dna	NOUN
american_scientific_journal-2520	16	33	sequence	sequence	NOUN
american_scientific_journal-2520	16	34	optimization	optimization	NOUN
american_scientific_journal-2520	16	35	for	for	ADP
american_scientific_journal-2520	16	36	maximization	maximization	NOUN
american_scientific_journal-2520	16	37	of	of	ADP
american_scientific_journal-2520	16	38	protein	protein	NOUN
american_scientific_journal-2520	16	39	yield	yield	NOUN
american_scientific_journal-2520	16	40	(	(	PUNCT
american_scientific_journal-2520	16	41	codons	codon	NOUN
american_scientific_journal-2520	16	42	need	need	VERB
american_scientific_journal-2520	16	43	to	to	PART
american_scientific_journal-2520	16	44	be	be	AUX
american_scientific_journal-2520	16	45	optimized	optimize	VERB
american_scientific_journal-2520	16	46	for	for	ADP
american_scientific_journal-2520	16	47	expression	expression	NOUN
american_scientific_journal-2520	16	48	in	in	ADP
american_scientific_journal-2520	16	49	your	your	PRON
american_scientific_journal-2520	16	50	preferred	preferred	ADJ
american_scientific_journal-2520	16	51	expression	expression	NOUN
american_scientific_journal-2520	16	52	system	system	NOUN
american_scientific_journal-2520	16	53	,	,	PUNCT
american_scientific_journal-2520	16	54	e.g.	e.g.	ADV
american_scientific_journal-2520	16	55	e.coli	e.coli	NOUN
american_scientific_journal-2520	16	56	or	or	CCONJ
american_scientific_journal-2520	16	57	yeast	yeast	NOUN
american_scientific_journal-2520	16	58	)	)	PUNCT
american_scientific_journal-2520	17	1	[	[	X
american_scientific_journal-2520	17	2	4	4	NUM
american_scientific_journal-2520	17	3	]	]	PUNCT
american_scientific_journal-2520	17	4	.	.	PUNCT
american_scientific_journal-2520	18	1	oligonucleotides	oligonucleotide	NOUN
american_scientific_journal-2520	18	2	are	be	AUX
american_scientific_journal-2520	18	3	the	the	DET
american_scientific_journal-2520	18	4	building	building	NOUN
american_scientific_journal-2520	18	5	blocks	block	NOUN
american_scientific_journal-2520	18	6	for	for	ADP
american_scientific_journal-2520	18	7	gene	gene	NOUN
american_scientific_journal-2520	18	8	synthesis	synthesis	NOUN
american_scientific_journal-2520	18	9	and	and	CCONJ
american_scientific_journal-2520	18	10	since	since	SCONJ
american_scientific_journal-2520	18	11	the	the	DET
american_scientific_journal-2520	18	12	synthesis	synthesis	NOUN
american_scientific_journal-2520	18	13	of	of	ADP
american_scientific_journal-2520	18	14	the	the	DET
american_scientific_journal-2520	18	15	first	first	ADJ
american_scientific_journal-2520	18	16	synthetic	synthetic	ADJ
american_scientific_journal-2520	18	17	gene	gene	NOUN
american_scientific_journal-2520	18	18	till	till	SCONJ
american_scientific_journal-2520	18	19	now	now	ADV
american_scientific_journal-2520	18	20	there	there	PRON
american_scientific_journal-2520	18	21	is	be	VERB
american_scientific_journal-2520	18	22	a	a	DET
american_scientific_journal-2520	18	23	several	several	ADJ
american_scientific_journal-2520	18	24	methods	method	NOUN
american_scientific_journal-2520	18	25	for	for	ADP
american_scientific_journal-2520	18	26	the	the	DET
american_scientific_journal-2520	18	27	synthesis	synthesis	NOUN
american_scientific_journal-2520	18	28	and	and	CCONJ
american_scientific_journal-2520	18	29	assembly	assembly	NOUN
american_scientific_journal-2520	18	30	of	of	ADP
american_scientific_journal-2520	18	31	dna	dna	PROPN
american_scientific_journal-2520	18	32	sequences	sequence	NOUN
american_scientific_journal-2520	18	33	based	base	VERB
american_scientific_journal-2520	18	34	on	on	ADP
american_scientific_journal-2520	18	35	oligonucleotides	oligonucleotide	NOUN
american_scientific_journal-2520	18	36	have	have	AUX
american_scientific_journal-2520	18	37	been	be	AUX
american_scientific_journal-2520	18	38	described	describe	VERB
american_scientific_journal-2520	18	39	,	,	PUNCT
american_scientific_journal-2520	18	40	a	a	DET
american_scientific_journal-2520	18	41	method	method	NOUN
american_scientific_journal-2520	18	42	for	for	ADP
american_scientific_journal-2520	18	43	enzymatic	enzymatic	ADJ
american_scientific_journal-2520	18	44	ligation	ligation	NOUN
american_scientific_journal-2520	18	45	of	of	ADP
american_scientific_journal-2520	18	46	oligonucleotides	oligonucleotide	NOUN
american_scientific_journal-2520	18	47	reported	report	VERB
american_scientific_journal-2520	18	48	in	in	ADP
american_scientific_journal-2520	18	49	the	the	DET
american_scientific_journal-2520	18	50	1980s	1980	NOUN
american_scientific_journal-2520	18	51	,	,	PUNCT
american_scientific_journal-2520	18	52	the	the	DET
american_scientific_journal-2520	18	53	foki	foki	ADJ
american_scientific_journal-2520	18	54	method	method	NOUN
american_scientific_journal-2520	18	55	published	publish	VERB
american_scientific_journal-2520	18	56	in1988	in1988	NOUN
american_scientific_journal-2520	19	1	[	[	X
american_scientific_journal-2520	19	2	5	5	NUM
american_scientific_journal-2520	19	3	]	]	PUNCT
american_scientific_journal-2520	19	4	,	,	PUNCT
american_scientific_journal-2520	19	5	self	self	NOUN
american_scientific_journal-2520	19	6	-	-	PUNCT
american_scientific_journal-2520	19	7	priming	prime	VERB
american_scientific_journal-2520	19	8	pcr	pcr	NOUN
american_scientific_journal-2520	19	9	,	,	PUNCT
american_scientific_journal-2520	19	10	the	the	DET
american_scientific_journal-2520	19	11	pcr	pcr	PROPN
american_scientific_journal-2520	19	12	assembly	assembly	NOUN
american_scientific_journal-2520	19	13	method	method	NOUN
american_scientific_journal-2520	20	1	[	[	X
american_scientific_journal-2520	20	2	6	6	NUM
american_scientific_journal-2520	20	3	]	]	PUNCT
american_scientific_journal-2520	20	4	,	,	PUNCT
american_scientific_journal-2520	20	5	the	the	DET
american_scientific_journal-2520	20	6	template	template	NOUN
american_scientific_journal-2520	20	7	directed	direct	VERB
american_scientific_journal-2520	20	8	ligation(tdl	ligation(tdl	PROPN
american_scientific_journal-2520	20	9	)	)	PUNCT
american_scientific_journal-2520	20	10	method	method	NOUN
american_scientific_journal-2520	20	11	,	,	PUNCT
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american_scientific_journal-2520	20	13	balanced	balance	VERB
american_scientific_journal-2520	20	14	inside	inside	NOUN
american_scientific_journal-2520	20	15	-	-	PUNCT
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american_scientific_journal-2520	20	17	(	(	PUNCT
american_scientific_journal-2520	20	18	tbio	tbio	ADJ
american_scientific_journal-2520	20	19	)	)	PUNCT
american_scientific_journal-2520	20	20	pcr	pcr	NOUN
american_scientific_journal-2520	20	21	-	-	PUNCT
american_scientific_journal-2520	20	22	based	base	VERB
american_scientific_journal-2520	20	23	gene	gene	NOUN
american_scientific_journal-2520	20	24	synthesis	synthesis	NOUN
american_scientific_journal-2520	20	25	method[7	method[7	NOUN
american_scientific_journal-2520	20	26	]	]	PUNCT
american_scientific_journal-2520	20	27	and	and	CCONJ
american_scientific_journal-2520	20	28	improved	improve	VERB
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american_scientific_journal-2520	20	30	of	of	ADP
american_scientific_journal-2520	20	31	the	the	DET
american_scientific_journal-2520	20	32	assembly	assembly	NOUN
american_scientific_journal-2520	20	33	pcr	pcr	PROPN
american_scientific_journal-2520	20	34	method	method	NOUN
american_scientific_journal-2520	20	35	described	describe	VERB
american_scientific_journal-2520	20	36	by	by	ADP
american_scientific_journal-2520	20	37	smith	smith	PROPN
american_scientific_journal-2520	20	38	and	and	CCONJ
american_scientific_journal-2520	20	39	his	his	PRON
american_scientific_journal-2520	20	40	colleagues	colleague	NOUN
american_scientific_journal-2520	20	41	[	[	X
american_scientific_journal-2520	20	42	8	8	NUM
american_scientific_journal-2520	20	43	;	;	PUNCT
american_scientific_journal-2520	20	44	9	9	NUM
american_scientific_journal-2520	20	45	]	]	PUNCT
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american_scientific_journal-2520	20	47	there	there	PRON
american_scientific_journal-2520	20	48	is	be	VERB
american_scientific_journal-2520	20	49	a	a	DET
american_scientific_journal-2520	20	50	many	many	ADJ
american_scientific_journal-2520	20	51	other	other	ADJ
american_scientific_journal-2520	20	52	gene	gene	NOUN
american_scientific_journal-2520	20	53	synthesis	synthesis	NOUN
american_scientific_journal-2520	20	54	approaches	approach	NOUN
american_scientific_journal-2520	20	55	like	like	ADP
american_scientific_journal-2520	20	56	two	two	NUM
american_scientific_journal-2520	20	57	-	-	PUNCT
american_scientific_journal-2520	20	58	step	step	NOUN
american_scientific_journal-2520	20	59	total	total	ADJ
american_scientific_journal-2520	20	60	gene	gene	NOUN
american_scientific_journal-2520	20	61	synthesis	synthesis	NOUN
american_scientific_journal-2520	20	62	using	use	VERB
american_scientific_journal-2520	20	63	both	both	DET
american_scientific_journal-2520	20	64	dual	dual	ADJ
american_scientific_journal-2520	20	65	asymmetrical	asymmetrical	ADJ
american_scientific_journal-2520	20	66	pcr	pcr	NOUN
american_scientific_journal-2520	20	67	(	(	PUNCT
american_scientific_journal-2520	20	68	da	da	ADJ
american_scientific_journal-2520	20	69	-	-	ADJ
american_scientific_journal-2520	20	70	pcr	pcr	ADJ
american_scientific_journal-2520	20	71	)	)	PUNCT
american_scientific_journal-2520	20	72	,	,	PUNCT
american_scientific_journal-2520	20	73	pcr	pcr	NOUN
american_scientific_journal-2520	20	74	-	-	PUNCT
american_scientific_journal-2520	20	75	based	base	VERB
american_scientific_journal-2520	20	76	two	two	NUM
american_scientific_journal-2520	20	77	-	-	PUNCT
american_scientific_journal-2520	20	78	step	step	NOUN
american_scientific_journal-2520	20	79	dna	dna	NOUN
american_scientific_journal-2520	20	80	synthesis	synthesis	NOUN
american_scientific_journal-2520	20	81	(	(	PUNCT
american_scientific_journal-2520	20	82	ptds	ptds	NOUN
american_scientific_journal-2520	20	83	)	)	PUNCT
american_scientific_journal-2520	21	1	[	[	X
american_scientific_journal-2520	21	2	9	9	NUM
american_scientific_journal-2520	21	3	]	]	PUNCT
american_scientific_journal-2520	21	4	,	,	PUNCT
american_scientific_journal-2520	21	5	and	and	CCONJ
american_scientific_journal-2520	21	6	overlap	overlap	NOUN
american_scientific_journal-2520	21	7	-	-	PUNCT
american_scientific_journal-2520	21	8	extension	extension	NOUN
american_scientific_journal-2520	21	9	pcr	pcr	NOUN
american_scientific_journal-2520	21	10	(	(	PUNCT
american_scientific_journal-2520	21	11	oe	oe	NOUN
american_scientific_journal-2520	21	12	-	-	PUNCT
american_scientific_journal-2520	21	13	pcr	pcr	NOUN
american_scientific_journal-2520	21	14	)	)	PUNCT
american_scientific_journal-2520	21	15	,	,	PUNCT
american_scientific_journal-2520	21	16	which	which	PRON
american_scientific_journal-2520	21	17	uses	use	VERB
american_scientific_journal-2520	21	18	a	a	DET
american_scientific_journal-2520	21	19	novel	novel	ADJ
american_scientific_journal-2520	21	20	method	method	NOUN
american_scientific_journal-2520	21	21	of	of	ADP
american_scientific_journal-2520	21	22	primer	primer	NOUN
american_scientific_journal-2520	21	23	design	design	NOUN
american_scientific_journal-2520	21	24	to	to	PART
american_scientific_journal-2520	21	25	achieve	achieve	VERB
american_scientific_journal-2520	21	26	high	high	ADJ
american_scientific_journal-2520	21	27	-	-	PUNCT
american_scientific_journal-2520	21	28	fidelity	fidelity	NOUN
american_scientific_journal-2520	21	29	assembly	assembly	NOUN
american_scientific_journal-2520	21	30	of	of	ADP
american_scientific_journal-2520	21	31	long	long	ADJ
american_scientific_journal-2520	21	32	gene	gene	NOUN
american_scientific_journal-2520	21	33	sequences	sequence	NOUN
american_scientific_journal-2520	22	1	[	[	X
american_scientific_journal-2520	22	2	8	8	NUM
american_scientific_journal-2520	22	3	]	]	PUNCT
american_scientific_journal-2520	22	4	.	.	PUNCT
american_scientific_journal-2520	23	1	pcr	pcr	NOUN
american_scientific_journal-2520	23	2	-	-	PUNCT
american_scientific_journal-2520	23	3	based	base	VERB
american_scientific_journal-2520	23	4	method	method	NOUN
american_scientific_journal-2520	23	5	of	of	ADP
american_scientific_journal-2520	23	6	recombining	recombine	VERB
american_scientific_journal-2520	23	7	dna	dna	NOUN
american_scientific_journal-2520	23	8	sequences	sequence	NOUN
american_scientific_journal-2520	23	9	[	[	X
american_scientific_journal-2520	23	10	10	10	NUM
american_scientific_journal-2520	23	11	]	]	X
american_scientific_journal-2520	23	12	it	it	PRON
american_scientific_journal-2520	23	13	is	be	AUX
american_scientific_journal-2520	23	14	a	a	DET
american_scientific_journal-2520	23	15	simple	simple	ADJ
american_scientific_journal-2520	23	16	,	,	PUNCT
american_scientific_journal-2520	23	17	versatile	versatile	ADJ
american_scientific_journal-2520	23	18	technique	technique	NOUN
american_scientific_journal-2520	23	19	for	for	ADP
american_scientific_journal-2520	23	20	gene	gene	NOUN
american_scientific_journal-2520	23	21	synthesis	synthesis	NOUN
american_scientific_journal-2520	23	22	[	[	X
american_scientific_journal-2520	23	23	11	11	NUM
american_scientific_journal-2520	23	24	]	]	PUNCT
american_scientific_journal-2520	23	25	described	describe	VERB
american_scientific_journal-2520	23	26	in	in	ADP
american_scientific_journal-2520	23	27	a	a	DET
american_scientific_journal-2520	23	28	biotechniques	biotechnique	NOUN
american_scientific_journal-2520	23	29	research	research	NOUN
american_scientific_journal-2520	23	30	report	report	NOUN
american_scientific_journal-2520	23	31	27	27	NUM
american_scientific_journal-2520	23	32	years	year	NOUN
american_scientific_journal-2520	23	33	ago	ago	ADV
american_scientific_journal-2520	23	34	.	.	PUNCT
american_scientific_journal-2520	24	1	and	and	CCONJ
american_scientific_journal-2520	24	2	still	still	ADV
american_scientific_journal-2520	24	3	attractive	attractive	ADJ
american_scientific_journal-2520	24	4	as	as	ADP
american_scientific_journal-2520	24	5	one	one	NUM
american_scientific_journal-2520	24	6	of	of	ADP
american_scientific_journal-2520	24	7	the	the	DET
american_scientific_journal-2520	24	8	more	more	ADV
american_scientific_journal-2520	24	9	important	important	ADJ
american_scientific_journal-2520	24	10	method	method	NOUN
american_scientific_journal-2520	24	11	for	for	ADP
american_scientific_journal-2520	24	12	gene	gene	NOUN
american_scientific_journal-2520	24	13	synthesis	synthesis	NOUN
american_scientific_journal-2520	24	14	.	.	PUNCT
american_scientific_journal-2520	25	1	the	the	DET
american_scientific_journal-2520	25	2	aim	aim	NOUN
american_scientific_journal-2520	25	3	of	of	ADP
american_scientific_journal-2520	25	4	current	current	ADJ
american_scientific_journal-2520	25	5	study	study	NOUN
american_scientific_journal-2520	25	6	was	be	AUX
american_scientific_journal-2520	25	7	to	to	PART
american_scientific_journal-2520	25	8	use	use	VERB
american_scientific_journal-2520	25	9	five	five	NUM
american_scientific_journal-2520	25	10	long	long	ADJ
american_scientific_journal-2520	25	11	oligonucleotides	oligonucleotide	NOUN
american_scientific_journal-2520	25	12	for	for	ADP
american_scientific_journal-2520	25	13	construction	construction	NOUN
american_scientific_journal-2520	25	14	of	of	ADP
american_scientific_journal-2520	25	15	active	active	ADJ
american_scientific_journal-2520	25	16	hpth	hpth	NOUN
american_scientific_journal-2520	25	17	gene	gene	NOUN
american_scientific_journal-2520	25	18	and	and	CCONJ
american_scientific_journal-2520	25	19	detect	detect	VERB
american_scientific_journal-2520	25	20	the	the	DET
american_scientific_journal-2520	25	21	ability	ability	NOUN
american_scientific_journal-2520	25	22	for	for	ADP
american_scientific_journal-2520	25	23	gene	gene	NOUN
american_scientific_journal-2520	25	24	transformation	transformation	NOUN
american_scientific_journal-2520	25	25	and	and	CCONJ
american_scientific_journal-2520	25	26	expression	expression	NOUN
american_scientific_journal-2520	25	27	in	in	ADP
american_scientific_journal-2520	25	28	bacterial	bacterial	ADJ
american_scientific_journal-2520	25	29	system	system	NOUN
american_scientific_journal-2520	25	30	.	.	PUNCT
american_scientific_journal-2520	26	1	2	2	X
american_scientific_journal-2520	26	2	.	.	X
american_scientific_journal-2520	26	3	materials	material	NOUN
american_scientific_journal-2520	26	4	and	and	CCONJ
american_scientific_journal-2520	26	5	methods	method	NOUN
american_scientific_journal-2520	26	6	the	the	DET
american_scientific_journal-2520	26	7	five	five	NUM
american_scientific_journal-2520	26	8	oligonucleotides	oligonucleotide	NOUN
american_scientific_journal-2520	26	9	,	,	PUNCT
american_scientific_journal-2520	26	10	random	random	ADJ
american_scientific_journal-2520	26	11	primer	primer	NOUN
american_scientific_journal-2520	26	12	and	and	CCONJ
american_scientific_journal-2520	26	13	all	all	DET
american_scientific_journal-2520	26	14	primers	primer	NOUN
american_scientific_journal-2520	26	15	used	use	VERB
american_scientific_journal-2520	26	16	in	in	ADP
american_scientific_journal-2520	26	17	this	this	DET
american_scientific_journal-2520	26	18	study	study	NOUN
american_scientific_journal-2520	26	19	were	be	AUX
american_scientific_journal-2520	26	20	supplied	supply	VERB
american_scientific_journal-2520	26	21	by	by	ADP
american_scientific_journal-2520	26	22	bioneer	bioneer	NOUN
american_scientific_journal-2520	26	23	company	company	NOUN
american_scientific_journal-2520	26	24	,	,	PUNCT
american_scientific_journal-2520	26	25	korea	korea	PROPN
american_scientific_journal-2520	26	26	.	.	PUNCT
american_scientific_journal-2520	27	1	the	the	DET
american_scientific_journal-2520	27	2	hindiii	hindiii	ADJ
american_scientific_journal-2520	27	3	restriction	restriction	NOUN
american_scientific_journal-2520	27	4	enzyme	enzyme	NOUN
american_scientific_journal-2520	27	5	was	be	AUX
american_scientific_journal-2520	27	6	purchased	purchase	VERB
american_scientific_journal-2520	27	7	from	from	ADP
american_scientific_journal-2520	27	8	(	(	PUNCT
american_scientific_journal-2520	27	9	promega	promega	PROPN
american_scientific_journal-2520	27	10	)	)	PUNCT
american_scientific_journal-2520	27	11	,	,	PUNCT
american_scientific_journal-2520	27	12	usa	usa	PROPN
american_scientific_journal-2520	27	13	,	,	PUNCT
american_scientific_journal-2520	27	14	and	and	CCONJ
american_scientific_journal-2520	27	15	kpni	kpni	VERB
american_scientific_journal-2520	27	16	from	from	ADP
american_scientific_journal-2520	27	17	invitrogen	invitrogen	PROPN
american_scientific_journal-2520	27	18	,	,	PUNCT
american_scientific_journal-2520	27	19	usa	usa	PROPN
american_scientific_journal-2520	27	20	,	,	PUNCT
american_scientific_journal-2520	27	21	the	the	DET
american_scientific_journal-2520	27	22	pet32	pet32	PROPN
american_scientific_journal-2520	27	23	a	a	DET
american_scientific_journal-2520	27	24	(	(	PUNCT
american_scientific_journal-2520	27	25	+	+	NOUN
american_scientific_journal-2520	27	26	)	)	PUNCT
american_scientific_journal-2520	27	27	vector	vector	NOUN
american_scientific_journal-2520	27	28	as	as	ADV
american_scientific_journal-2520	27	29	well	well	ADV
american_scientific_journal-2520	27	30	as	as	ADP
american_scientific_journal-2520	27	31	e.	e.	PROPN
american_scientific_journal-2520	27	32	coli	coli	NOUN
american_scientific_journal-2520	27	33	strain	strain	NOUN
american_scientific_journal-2520	28	1	bl21	bl21	PROPN
american_scientific_journal-2520	28	2	de3	de3	NOUN
american_scientific_journal-2520	28	3	were	be	AUX
american_scientific_journal-2520	28	4	purchased	purchase	VERB
american_scientific_journal-2520	28	5	from	from	ADP
american_scientific_journal-2520	28	6	(	(	PUNCT
american_scientific_journal-2520	28	7	novagen	novagen	PROPN
american_scientific_journal-2520	28	8	)	)	PUNCT
american_scientific_journal-2520	28	9	usa	usa	PROPN
american_scientific_journal-2520	28	10	and	and	CCONJ
american_scientific_journal-2520	28	11	the	the	DET
american_scientific_journal-2520	28	12	other	other	ADJ
american_scientific_journal-2520	28	13	e.	e.	PROPN
american_scientific_journal-2520	28	14	coli	coli	PROPN
american_scientific_journal-2520	28	15	strain	strain	NOUN
american_scientific_journal-2520	28	16	dh5α	dh5α	PROPN
american_scientific_journal-2520	28	17	were	be	AUX
american_scientific_journal-2520	28	18	purchased	purchase	VERB
american_scientific_journal-2520	28	19	from	from	ADP
american_scientific_journal-2520	28	20	(	(	PUNCT
american_scientific_journal-2520	28	21	invtrogen	invtrogen	PROPN
american_scientific_journal-2520	28	22	)	)	PUNCT
american_scientific_journal-2520	28	23	usa	usa	PROPN
american_scientific_journal-2520	28	24	,	,	PUNCT
american_scientific_journal-2520	28	25	all	all	DET
american_scientific_journal-2520	28	26	other	other	ADJ
american_scientific_journal-2520	28	27	materials	material	NOUN
american_scientific_journal-2520	28	28	and	and	CCONJ
american_scientific_journal-2520	28	29	kits	kit	NOUN
american_scientific_journal-2520	28	30	supplied	supply	VERB
american_scientific_journal-2520	28	31	by	by	ADP
american_scientific_journal-2520	28	32	bioneer	bioneer	NOUN
american_scientific_journal-2520	28	33	or	or	CCONJ
american_scientific_journal-2520	28	34	promega	promega	PROPN
american_scientific_journal-2520	28	35	as	as	SCONJ
american_scientific_journal-2520	28	36	pointed	point	VERB
american_scientific_journal-2520	28	37	below	below	ADV
american_scientific_journal-2520	28	38	.	.	PUNCT
american_scientific_journal-2520	29	1	2.1	2.1	NUM
american_scientific_journal-2520	29	2	.	.	PUNCT
american_scientific_journal-2520	30	1	construction	construction	NOUN
american_scientific_journal-2520	30	2	of	of	ADP
american_scientific_journal-2520	30	3	synthetic	synthetic	ADJ
american_scientific_journal-2520	30	4	recombinant	recombinant	ADJ
american_scientific_journal-2520	30	5	human	human	ADJ
american_scientific_journal-2520	30	6	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	30	7	hormone	hormone	NOUN
american_scientific_journal-2520	30	8	(	(	PUNCT
american_scientific_journal-2520	30	9	1	1	NUM
american_scientific_journal-2520	30	10	-	-	NUM
american_scientific_journal-2520	30	11	84	84	NUM
american_scientific_journal-2520	30	12	)	)	PUNCT
american_scientific_journal-2520	30	13	gene	gene	NOUN
american_scientific_journal-2520	30	14	(	(	PUNCT
american_scientific_journal-2520	30	15	shpth	shpth	ADJ
american_scientific_journal-2520	30	16	)	)	PUNCT
american_scientific_journal-2520	30	17	american	american	ADJ
american_scientific_journal-2520	30	18	scientific	scientific	ADJ
american_scientific_journal-2520	30	19	research	research	NOUN
american_scientific_journal-2520	30	20	journal	journal	NOUN
american_scientific_journal-2520	30	21	for	for	ADP
american_scientific_journal-2520	30	22	engineering	engineering	NOUN
american_scientific_journal-2520	30	23	,	,	PUNCT
american_scientific_journal-2520	30	24	technology	technology	NOUN
american_scientific_journal-2520	30	25	,	,	PUNCT
american_scientific_journal-2520	30	26	and	and	CCONJ
american_scientific_journal-2520	30	27	sciences	science	NOUN
american_scientific_journal-2520	30	28	(	(	PUNCT
american_scientific_journal-2520	30	29	asrjets	asrjet	NOUN
american_scientific_journal-2520	30	30	)	)	PUNCT
american_scientific_journal-2520	30	31	(	(	PUNCT
american_scientific_journal-2520	30	32	2017	2017	NUM
american_scientific_journal-2520	30	33	)	)	PUNCT
american_scientific_journal-2520	30	34	volume	volume	NOUN
american_scientific_journal-2520	30	35	27	27	NUM
american_scientific_journal-2520	30	36	,	,	PUNCT
american_scientific_journal-2520	30	37	no	no	DET
american_scientific_journal-2520	30	38	1	1	NUM
american_scientific_journal-2520	30	39	,	,	PUNCT
american_scientific_journal-2520	30	40	pp	pp	ADV
american_scientific_journal-2520	30	41	12	12	NUM
american_scientific_journal-2520	30	42	-	-	SYM
american_scientific_journal-2520	30	43	24	24	NUM
american_scientific_journal-2520	30	44	14	14	NUM
american_scientific_journal-2520	30	45	the	the	DET
american_scientific_journal-2520	30	46	gene	gene	NOUN
american_scientific_journal-2520	30	47	was	be	AUX
american_scientific_journal-2520	30	48	constructed	construct	VERB
american_scientific_journal-2520	30	49	by	by	ADP
american_scientific_journal-2520	30	50	gene	gene	NOUN
american_scientific_journal-2520	30	51	splicing	splicing	NOUN
american_scientific_journal-2520	30	52	by	by	ADP
american_scientific_journal-2520	30	53	overlap	overlap	NOUN
american_scientific_journal-2520	30	54	extension	extension	NOUN
american_scientific_journal-2520	30	55	pcr	pcr	NOUN
american_scientific_journal-2520	30	56	(	(	PUNCT
american_scientific_journal-2520	30	57	soe	soe	PROPN
american_scientific_journal-2520	30	58	pcr	pcr	PROPN
american_scientific_journal-2520	30	59	)	)	PUNCT
american_scientific_journal-2520	30	60	using	use	VERB
american_scientific_journal-2520	30	61	the	the	DET
american_scientific_journal-2520	30	62	following	follow	VERB
american_scientific_journal-2520	30	63	primers	primer	NOUN
american_scientific_journal-2520	30	64	which	which	PRON
american_scientific_journal-2520	30	65	given	give	VERB
american_scientific_journal-2520	30	66	by	by	ADP
american_scientific_journal-2520	30	67	liu	liu	PROPN
american_scientific_journal-2520	30	68	and	and	CCONJ
american_scientific_journal-2520	30	69	his	his	PRON
american_scientific_journal-2520	30	70	colleagues	colleague	NOUN
american_scientific_journal-2520	31	1	[	[	X
american_scientific_journal-2520	31	2	12	12	NUM
american_scientific_journal-2520	31	3	]	]	X
american_scientific_journal-2520	31	4	:	:	PUNCT
american_scientific_journal-2520	31	5	primer	primer	NOUN
american_scientific_journal-2520	31	6	1	1	NUM
american_scientific_journal-2520	31	7	:	:	SYM
american_scientific_journal-2520	31	8	5	5	NUM
american_scientific_journal-2520	31	9	'	'	PUNCT
american_scientific_journal-2520	31	10	ctgggtaccgatgacgatg	ctgggtaccgatgacgatg	PROPN
american_scientific_journal-2520	31	11	ac	ac	PROPN
american_scientific_journal-2520	31	12	aagtctgtgagtgaaattcagctgatgcataacctggggaaacatctgaact-3	aagtctgtgagtgaaattcagctgatgcataacctggggaaacatctgaact-3	PROPN
american_scientific_journal-2520	31	13	'	'	PUNCT
american_scientific_journal-2520	31	14	,	,	PUNCT
american_scientific_journal-2520	31	15	primer	primer	NOUN
american_scientific_journal-2520	31	16	2	2	NUM
american_scientific_journal-2520	31	17	:	:	PUNCT
american_scientific_journal-2520	31	18	5'aa	5'aa	NUM
american_scientific_journal-2520	31	19	attgtgcacatcctgcagcttcttacgcagccattctacacgctccatcgagttcagatgtttcccc	attgtgcacatcctgcagcttcttacgcagccattctacacgctccatcgagttcagatgtttcccc	PROPN
american_scientific_journal-2520	31	20	agg3	agg3	PROPN
american_scientific_journal-2520	31	21	,	,	PUNCT
american_scientific_journal-2520	31	22	primer	primer	NOUN
american_scientific_journal-2520	31	23	3	3	NUM
american_scientific_journal-2520	31	24	:	:	PUNCT
american_scientific_journal-2520	31	25	5'tacgcggacgctgggaaccagcatcgcgcggaccagcggggcacccagggcaacaaaattgtgc	5'tacgcggacgctgggaaccagcatcgcgcggaccagcggggcacccagggcaacaaaattgtgc	NUM
american_scientific_journal-2520	31	26	acatcctgca-3	acatcctgca-3	NOUN
american_scientific_journal-2520	31	27	'	'	PART
american_scientific_journal-2520	31	28	,	,	PUNCT
american_scientific_journal-2520	31	29	primer	primer	NOUN
american_scientific_journal-2520	31	30	4	4	NUM
american_scientific_journal-2520	31	31	:	:	PUNCT
american_scientific_journal-2520	31	32	5'tctgcc	5'tctgcc	NUM
american_scientific_journal-2520	31	33	tcgcccag	tcgcccag	NOUN
american_scientific_journal-2520	31	34	gcttttttcatggctctcaacca	gcttttttcatggctctcaacca	NOUN
american_scientific_journal-2520	31	35	agacattgtc	agacattgtc	PROPN
american_scientific_journal-2520	31	36	ttcct	ttcct	PROPN
american_scientific_journal-2520	31	37	ttttacgcgg	ttttacgcgg	NOUN
american_scientific_journal-2520	31	38	acg	acg	PROPN
american_scientific_journal-2520	31	39	ctgggaacca-3	ctgggaacca-3	PROPN
american_scientific_journal-2520	31	40	'	'	PART
american_scientific_journal-2520	31	41	,	,	PUNCT
american_scientific_journal-2520	31	42	primer5	primer5	NOUN
american_scientific_journal-2520	31	43	:	:	PUNCT
american_scientific_journal-2520	31	44	5'-cgcaagcttatcactgggatttagctttagttaataca	5'-cgcaagcttatcactgggatttagctttagttaataca	NUM
american_scientific_journal-2520	31	45	ttcacatcggctttgtctgcctcgcccaggctttt-3	ttcacatcggctttgtctgcctcgcccaggctttt-3	NOUN
american_scientific_journal-2520	31	46	'	'	PUNCT
american_scientific_journal-2520	31	47	.	.	PUNCT
american_scientific_journal-2520	32	1	at	at	ADP
american_scientific_journal-2520	32	2	this	this	DET
american_scientific_journal-2520	32	3	primers	primer	NOUN
american_scientific_journal-2520	32	4	for	for	ADP
american_scientific_journal-2520	32	5	the	the	DET
american_scientific_journal-2520	32	6	preferential	preferential	ADJ
american_scientific_journal-2520	32	7	codons	codon	NOUN
american_scientific_journal-2520	32	8	of	of	ADP
american_scientific_journal-2520	32	9	e.	e.	PROPN
american_scientific_journal-2520	32	10	coli	coli	PROPN
american_scientific_journal-2520	32	11	,	,	PUNCT
american_scientific_journal-2520	32	12	were	be	AUX
american_scientific_journal-2520	32	13	used	use	VERB
american_scientific_journal-2520	32	14	and	and	CCONJ
american_scientific_journal-2520	32	15	designed	design	VERB
american_scientific_journal-2520	32	16	with	with	ADP
american_scientific_journal-2520	32	17	termination	termination	NOUN
american_scientific_journal-2520	32	18	codon	codon	NOUN
american_scientific_journal-2520	32	19	and	and	CCONJ
american_scientific_journal-2520	32	20	hindiii	hindiii	ADJ
american_scientific_journal-2520	32	21	recognition	recognition	NOUN
american_scientific_journal-2520	32	22	site	site	NOUN
american_scientific_journal-2520	32	23	at	at	ADP
american_scientific_journal-2520	32	24	c	c	NOUN
american_scientific_journal-2520	32	25	-	-	PUNCT
american_scientific_journal-2520	32	26	terminus	terminus	NOUN
american_scientific_journal-2520	32	27	as	as	ADV
american_scientific_journal-2520	32	28	well	well	ADV
american_scientific_journal-2520	32	29	as	as	ADP
american_scientific_journal-2520	32	30	a	a	DET
american_scientific_journal-2520	32	31	kpni	kpni	NOUN
american_scientific_journal-2520	32	32	recognition	recognition	NOUN
american_scientific_journal-2520	32	33	site	site	NOUN
american_scientific_journal-2520	32	34	and	and	CCONJ
american_scientific_journal-2520	32	35	an	an	DET
american_scientific_journal-2520	32	36	enterokinas	enterokina	NOUN
american_scientific_journal-2520	32	37	separation	separation	NOUN
american_scientific_journal-2520	32	38	site	site	NOUN
american_scientific_journal-2520	32	39	at	at	ADP
american_scientific_journal-2520	32	40	n	n	NOUN
american_scientific_journal-2520	32	41	-	-	PUNCT
american_scientific_journal-2520	32	42	terminus	terminus	NOUN
american_scientific_journal-2520	32	43	,	,	PUNCT
american_scientific_journal-2520	32	44	and	and	CCONJ
american_scientific_journal-2520	32	45	also	also	ADV
american_scientific_journal-2520	32	46	two	two	NUM
american_scientific_journal-2520	32	47	primers	primer	NOUN
american_scientific_journal-2520	32	48	were	be	AUX
american_scientific_journal-2520	32	49	used	use	VERB
american_scientific_journal-2520	32	50	to	to	PART
american_scientific_journal-2520	32	51	amplify	amplify	VERB
american_scientific_journal-2520	32	52	the	the	DET
american_scientific_journal-2520	32	53	whole	whole	ADJ
american_scientific_journal-2520	32	54	gene	gene	NOUN
american_scientific_journal-2520	32	55	after	after	ADP
american_scientific_journal-2520	32	56	construction	construction	NOUN
american_scientific_journal-2520	32	57	of	of	ADP
american_scientific_journal-2520	32	58	full	full	ADJ
american_scientific_journal-2520	32	59	length	length	NOUN
american_scientific_journal-2520	32	60	of	of	ADP
american_scientific_journal-2520	32	61	gene	gene	NOUN
american_scientific_journal-2520	32	62	,	,	PUNCT
american_scientific_journal-2520	32	63	f	f	PROPN
american_scientific_journal-2520	32	64	primer	primer	NOUN
american_scientific_journal-2520	32	65	5`-ctgggtaccgatgacgatg-3	5`-ctgggtaccgatgacgatg-3	NOUN
american_scientific_journal-2520	32	66	'	'	PUNCT
american_scientific_journal-2520	32	67	and	and	CCONJ
american_scientific_journal-2520	32	68	r	r	NOUN
american_scientific_journal-2520	32	69	primer	primer	NOUN
american_scientific_journal-2520	32	70	5'cgcaagctta	5'cgcaagctta	NUM
american_scientific_journal-2520	32	71	tcac	tcac	NUM
american_scientific_journal-2520	32	72	gggattta-3	gggattta-3	NOUN
american_scientific_journal-2520	32	73	'	'	NUM
american_scientific_journal-2520	32	74	.	.	PUNCT
american_scientific_journal-2520	33	1	the	the	DET
american_scientific_journal-2520	33	2	primers	primer	NOUN
american_scientific_journal-2520	33	3	or	or	CCONJ
american_scientific_journal-2520	33	4	oligonucleotide	oligonucleotide	NOUN
american_scientific_journal-2520	33	5	designed	design	VERB
american_scientific_journal-2520	33	6	to	to	PART
american_scientific_journal-2520	33	7	form	form	VERB
american_scientific_journal-2520	33	8	the	the	DET
american_scientific_journal-2520	33	9	gene	gene	NOUN
american_scientific_journal-2520	33	10	with	with	ADP
american_scientific_journal-2520	33	11	four	four	NUM
american_scientific_journal-2520	33	12	pcr	pcr	ADJ
american_scientific_journal-2520	33	13	cycles	cycle	NOUN
american_scientific_journal-2520	33	14	in	in	ADP
american_scientific_journal-2520	33	15	the	the	DET
american_scientific_journal-2520	33	16	first	first	ADJ
american_scientific_journal-2520	33	17	pcr	pcr	ADJ
american_scientific_journal-2520	33	18	cycle	cycle	NOUN
american_scientific_journal-2520	33	19	,	,	PUNCT
american_scientific_journal-2520	33	20	the	the	DET
american_scientific_journal-2520	33	21	first	first	ADJ
american_scientific_journal-2520	33	22	primer	primer	NOUN
american_scientific_journal-2520	33	23	and	and	CCONJ
american_scientific_journal-2520	33	24	second	second	ADJ
american_scientific_journal-2520	33	25	primer	primer	NOUN
american_scientific_journal-2520	33	26	were	be	AUX
american_scientific_journal-2520	33	27	used	use	VERB
american_scientific_journal-2520	33	28	as	as	ADP
american_scientific_journal-2520	33	29	sense	sense	NOUN
american_scientific_journal-2520	33	30	and	and	CCONJ
american_scientific_journal-2520	33	31	antisense	antisense	NOUN
american_scientific_journal-2520	33	32	primers	primer	NOUN
american_scientific_journal-2520	33	33	separately	separately	ADV
american_scientific_journal-2520	33	34	.	.	PUNCT
american_scientific_journal-2520	34	1	73	73	NUM
american_scientific_journal-2520	34	2	nucleotides	nucleotide	NOUN
american_scientific_journal-2520	34	3	of	of	ADP
american_scientific_journal-2520	34	4	primer	primer	NOUN
american_scientific_journal-2520	34	5	1	1	NUM
american_scientific_journal-2520	34	6	were	be	AUX
american_scientific_journal-2520	34	7	similar	similar	ADJ
american_scientific_journal-2520	34	8	to	to	ADP
american_scientific_journal-2520	34	9	the	the	DET
american_scientific_journal-2520	34	10	designed	design	VERB
american_scientific_journal-2520	34	11	nucleotides	nucleotide	NOUN
american_scientific_journal-2520	34	12	(	(	PUNCT
american_scientific_journal-2520	34	13	1–73	1–73	PROPN
american_scientific_journal-2520	34	14	)	)	PUNCT
american_scientific_journal-2520	34	15	of	of	ADP
american_scientific_journal-2520	34	16	the	the	DET
american_scientific_journal-2520	34	17	gene	gene	NOUN
american_scientific_journal-2520	34	18	.	.	PUNCT
american_scientific_journal-2520	35	1	while	while	SCONJ
american_scientific_journal-2520	35	2	,	,	PUNCT
american_scientific_journal-2520	35	3	nucleotides	nucleotide	NOUN
american_scientific_journal-2520	35	4	sequence	sequence	NOUN
american_scientific_journal-2520	35	5	of	of	ADP
american_scientific_journal-2520	35	6	second	second	ADJ
american_scientific_journal-2520	35	7	primer	primer	NOUN
american_scientific_journal-2520	35	8	was	be	AUX
american_scientific_journal-2520	35	9	complementary	complementary	ADJ
american_scientific_journal-2520	35	10	to	to	ADP
american_scientific_journal-2520	35	11	the	the	DET
american_scientific_journal-2520	35	12	designed	design	VERB
american_scientific_journal-2520	35	13	nucleotides	nucleotide	NOUN
american_scientific_journal-2520	35	14	(	(	PUNCT
american_scientific_journal-2520	35	15	54–126	54–126	PROPN
american_scientific_journal-2520	35	16	)	)	PUNCT
american_scientific_journal-2520	35	17	.	.	PUNCT
american_scientific_journal-2520	36	1	20	20	NUM
american_scientific_journal-2520	36	2	nucleotides	nucleotide	NOUN
american_scientific_journal-2520	36	3	of	of	ADP
american_scientific_journal-2520	36	4	the	the	DET
american_scientific_journal-2520	36	5	first	first	ADJ
american_scientific_journal-2520	36	6	primer	primer	NOUN
american_scientific_journal-2520	36	7	and	and	CCONJ
american_scientific_journal-2520	36	8	second	second	ADJ
american_scientific_journal-2520	36	9	primer	primer	NOUN
american_scientific_journal-2520	36	10	from	from	ADP
american_scientific_journal-2520	36	11	3	3	NUM
american_scientific_journal-2520	36	12	'	'	PART
american_scientific_journal-2520	36	13	terminus	terminus	NOUN
american_scientific_journal-2520	36	14	were	be	AUX
american_scientific_journal-2520	36	15	complementary	complementary	ADJ
american_scientific_journal-2520	36	16	(	(	PUNCT
american_scientific_journal-2520	36	17	overlapping	overlap	VERB
american_scientific_journal-2520	36	18	region	region	NOUN
american_scientific_journal-2520	36	19	)	)	PUNCT
american_scientific_journal-2520	36	20	.	.	PUNCT
american_scientific_journal-2520	37	1	so	so	ADV
american_scientific_journal-2520	37	2	,	,	PUNCT
american_scientific_journal-2520	37	3	they	they	PRON
american_scientific_journal-2520	37	4	could	could	AUX
american_scientific_journal-2520	37	5	be	be	AUX
american_scientific_journal-2520	37	6	used	use	VERB
american_scientific_journal-2520	37	7	as	as	ADP
american_scientific_journal-2520	37	8	a	a	DET
american_scientific_journal-2520	37	9	template	template	NOUN
american_scientific_journal-2520	37	10	and	and	CCONJ
american_scientific_journal-2520	37	11	primer	primer	NOUN
american_scientific_journal-2520	37	12	to	to	ADP
american_scientific_journal-2520	37	13	each	each	DET
american_scientific_journal-2520	37	14	other	other	ADJ
american_scientific_journal-2520	37	15	.	.	PUNCT
american_scientific_journal-2520	38	1	the	the	DET
american_scientific_journal-2520	38	2	sequence	sequence	NOUN
american_scientific_journal-2520	38	3	of	of	ADP
american_scientific_journal-2520	38	4	the	the	DET
american_scientific_journal-2520	38	5	first	first	ADJ
american_scientific_journal-2520	38	6	pcr	pcr	ADJ
american_scientific_journal-2520	38	7	production	production	NOUN
american_scientific_journal-2520	38	8	was	be	AUX
american_scientific_journal-2520	38	9	identical	identical	ADJ
american_scientific_journal-2520	38	10	to	to	ADP
american_scientific_journal-2520	38	11	the	the	DET
american_scientific_journal-2520	38	12	designed	design	VERB
american_scientific_journal-2520	38	13	nucleotides	nucleotide	NOUN
american_scientific_journal-2520	38	14	(	(	PUNCT
american_scientific_journal-2520	38	15	1–126	1–126	NUM
american_scientific_journal-2520	38	16	)	)	PUNCT
american_scientific_journal-2520	38	17	.	.	PUNCT
american_scientific_journal-2520	39	1	again	again	ADV
american_scientific_journal-2520	39	2	,	,	PUNCT
american_scientific_journal-2520	39	3	in	in	ADP
american_scientific_journal-2520	39	4	second	second	ADJ
american_scientific_journal-2520	39	5	pcr	pcr	NOUN
american_scientific_journal-2520	39	6	,	,	PUNCT
american_scientific_journal-2520	39	7	the	the	DET
american_scientific_journal-2520	39	8	first	first	ADJ
american_scientific_journal-2520	39	9	pcr	pcr	ADJ
american_scientific_journal-2520	39	10	product	product	NOUN
american_scientific_journal-2520	39	11	and	and	CCONJ
american_scientific_journal-2520	39	12	the	the	DET
american_scientific_journal-2520	39	13	third	third	ADJ
american_scientific_journal-2520	39	14	primer	primer	NOUN
american_scientific_journal-2520	39	15	were	be	AUX
american_scientific_journal-2520	39	16	used	use	VERB
american_scientific_journal-2520	39	17	as	as	ADP
american_scientific_journal-2520	39	18	a	a	DET
american_scientific_journal-2520	39	19	template	template	NOUN
american_scientific_journal-2520	39	20	and	and	CCONJ
american_scientific_journal-2520	39	21	primer	primer	NOUN
american_scientific_journal-2520	39	22	to	to	ADP
american_scientific_journal-2520	39	23	each	each	DET
american_scientific_journal-2520	39	24	other	other	ADJ
american_scientific_journal-2520	39	25	and	and	CCONJ
american_scientific_journal-2520	39	26	produced	produce	VERB
american_scientific_journal-2520	39	27	the	the	DET
american_scientific_journal-2520	39	28	designed	design	VERB
american_scientific_journal-2520	39	29	nucleotides	nucleotide	NOUN
american_scientific_journal-2520	39	30	(	(	PUNCT
american_scientific_journal-2520	39	31	1–181	1–181	NUM
american_scientific_journal-2520	39	32	)	)	PUNCT
american_scientific_journal-2520	39	33	.	.	PUNCT
american_scientific_journal-2520	40	1	and	and	CCONJ
american_scientific_journal-2520	40	2	the	the	DET
american_scientific_journal-2520	40	3	relaxation	relaxation	NOUN
american_scientific_journal-2520	40	4	pcrs	pcrs	NOUN
american_scientific_journal-2520	40	5	may	may	AUX
american_scientific_journal-2520	40	6	be	be	AUX
american_scientific_journal-2520	40	7	deduced	deduce	VERB
american_scientific_journal-2520	40	8	by	by	ADP
american_scientific_journal-2520	40	9	the	the	DET
american_scientific_journal-2520	40	10	same	same	ADJ
american_scientific_journal-2520	40	11	way	way	NOUN
american_scientific_journal-2520	40	12	.	.	PUNCT
american_scientific_journal-2520	41	1	finally	finally	ADV
american_scientific_journal-2520	41	2	,	,	PUNCT
american_scientific_journal-2520	41	3	the	the	DET
american_scientific_journal-2520	41	4	designed	design	VERB
american_scientific_journal-2520	41	5	oligonucleotide	oligonucleotide	NOUN
american_scientific_journal-2520	41	6	(	(	PUNCT
american_scientific_journal-2520	41	7	1–289	1–289	NUM
american_scientific_journal-2520	41	8	)	)	PUNCT
american_scientific_journal-2520	41	9	was	be	AUX
american_scientific_journal-2520	41	10	gained	gain	VERB
american_scientific_journal-2520	41	11	.	.	PUNCT
american_scientific_journal-2520	42	1	2.1.1	2.1.1	X
american_scientific_journal-2520	42	2	.	.	PUNCT
american_scientific_journal-2520	43	1	construction	construction	NOUN
american_scientific_journal-2520	43	2	and	and	CCONJ
american_scientific_journal-2520	43	3	amplification	amplification	NOUN
american_scientific_journal-2520	43	4	of	of	ADP
american_scientific_journal-2520	43	5	shpth	shpth	ADJ
american_scientific_journal-2520	43	6	gene	gene	NOUN
american_scientific_journal-2520	43	7	in	in	ADP
american_scientific_journal-2520	43	8	the	the	DET
american_scientific_journal-2520	43	9	building	building	NOUN
american_scientific_journal-2520	43	10	of	of	ADP
american_scientific_journal-2520	43	11	gene	gene	NOUN
american_scientific_journal-2520	43	12	the	the	DET
american_scientific_journal-2520	43	13	first	first	ADJ
american_scientific_journal-2520	43	14	pcr	pcr	ADJ
american_scientific_journal-2520	43	15	product	product	NOUN
american_scientific_journal-2520	43	16	gained	gain	VERB
american_scientific_journal-2520	43	17	by	by	ADP
american_scientific_journal-2520	43	18	using	use	VERB
american_scientific_journal-2520	43	19	gotaq	gotaq	PROPN
american_scientific_journal-2520	43	20	®	®	NOUN
american_scientific_journal-2520	43	21	green	green	ADJ
american_scientific_journal-2520	43	22	master	master	NOUN
american_scientific_journal-2520	43	23	mix	mix	NOUN
american_scientific_journal-2520	43	24	cat	cat	NOUN
american_scientific_journal-2520	43	25	.	.	PUNCT
american_scientific_journal-2520	44	1	#	#	NOUN
american_scientific_journal-2520	44	2	m7122	m7122	PROPN
american_scientific_journal-2520	44	3	(	(	PUNCT
american_scientific_journal-2520	44	4	promega	promega	PROPN
american_scientific_journal-2520	44	5	)	)	PUNCT
american_scientific_journal-2520	44	6	.	.	PUNCT
american_scientific_journal-2520	45	1	the	the	DET
american_scientific_journal-2520	45	2	pcr	pcr	PROPN
american_scientific_journal-2520	45	3	reaction	reaction	NOUN
american_scientific_journal-2520	45	4	tube	tube	NOUN
american_scientific_journal-2520	45	5	for	for	ADP
american_scientific_journal-2520	45	6	each	each	DET
american_scientific_journal-2520	45	7	pcr	pcr	ADJ
american_scientific_journal-2520	45	8	cycle	cycle	NOUN
american_scientific_journal-2520	45	9	contain	contain	VERB
american_scientific_journal-2520	45	10	the	the	DET
american_scientific_journal-2520	45	11	12.5µl	12.5µl	NUM
american_scientific_journal-2520	45	12	of	of	ADP
american_scientific_journal-2520	45	13	gotaq	gotaq	PROPN
american_scientific_journal-2520	45	14	®	®	NOUN
american_scientific_journal-2520	45	15	green	green	ADJ
american_scientific_journal-2520	45	16	master	master	NOUN
american_scientific_journal-2520	45	17	mix	mix	NOUN
american_scientific_journal-2520	45	18	as	as	ADV
american_scientific_journal-2520	45	19	well	well	ADV
american_scientific_journal-2520	45	20	as	as	ADP
american_scientific_journal-2520	45	21	sufficient	sufficient	ADJ
american_scientific_journal-2520	45	22	amount	amount	NOUN
american_scientific_journal-2520	45	23	of	of	ADP
american_scientific_journal-2520	45	24	nuclease	nuclease	NOUN
american_scientific_journal-2520	45	25	-	-	PUNCT
american_scientific_journal-2520	45	26	free	free	ADJ
american_scientific_journal-2520	45	27	water	water	NOUN
american_scientific_journal-2520	45	28	to	to	PART
american_scientific_journal-2520	45	29	complete	complete	VERB
american_scientific_journal-2520	45	30	the	the	DET
american_scientific_journal-2520	45	31	volume	volume	NOUN
american_scientific_journal-2520	45	32	to	to	ADP
american_scientific_journal-2520	45	33	25µl	25µl	NOUN
american_scientific_journal-2520	45	34	the	the	DET
american_scientific_journal-2520	45	35	other	other	ADJ
american_scientific_journal-2520	45	36	pcr	pcr	ADJ
american_scientific_journal-2520	45	37	tube	tube	NOUN
american_scientific_journal-2520	45	38	contents	content	NOUN
american_scientific_journal-2520	45	39	for	for	ADP
american_scientific_journal-2520	45	40	the	the	DET
american_scientific_journal-2520	45	41	first	first	ADJ
american_scientific_journal-2520	45	42	pcr	pcr	ADJ
american_scientific_journal-2520	45	43	cycle	cycle	NOUN
american_scientific_journal-2520	45	44	about	about	ADP
american_scientific_journal-2520	45	45	0.6µl	0.6µl	NUM
american_scientific_journal-2520	45	46	from	from	ADP
american_scientific_journal-2520	45	47	primer1	primer1	NOUN
american_scientific_journal-2520	45	48	and	and	CCONJ
american_scientific_journal-2520	45	49	primer2	primer2	PROPN
american_scientific_journal-2520	45	50	were	be	AUX
american_scientific_journal-2520	45	51	added	add	VERB
american_scientific_journal-2520	45	52	,	,	PUNCT
american_scientific_journal-2520	45	53	while	while	SCONJ
american_scientific_journal-2520	45	54	in	in	ADP
american_scientific_journal-2520	45	55	the	the	DET
american_scientific_journal-2520	45	56	second	second	ADJ
american_scientific_journal-2520	45	57	pcr	pcr	ADJ
american_scientific_journal-2520	45	58	cycle	cycle	NOUN
american_scientific_journal-2520	45	59	about	about	ADP
american_scientific_journal-2520	45	60	1µl	1µl	NOUN
american_scientific_journal-2520	45	61	of	of	ADP
american_scientific_journal-2520	45	62	american	american	PROPN
american_scientific_journal-2520	45	63	scientific	scientific	ADJ
american_scientific_journal-2520	45	64	research	research	NOUN
american_scientific_journal-2520	45	65	journal	journal	NOUN
american_scientific_journal-2520	45	66	for	for	ADP
american_scientific_journal-2520	45	67	engineering	engineering	NOUN
american_scientific_journal-2520	45	68	,	,	PUNCT
american_scientific_journal-2520	45	69	technology	technology	NOUN
american_scientific_journal-2520	45	70	,	,	PUNCT
american_scientific_journal-2520	45	71	and	and	CCONJ
american_scientific_journal-2520	45	72	sciences	science	NOUN
american_scientific_journal-2520	45	73	(	(	PUNCT
american_scientific_journal-2520	45	74	asrjets	asrjet	NOUN
american_scientific_journal-2520	45	75	)	)	PUNCT
american_scientific_journal-2520	45	76	(	(	PUNCT
american_scientific_journal-2520	45	77	2017	2017	NUM
american_scientific_journal-2520	45	78	)	)	PUNCT
american_scientific_journal-2520	45	79	volume	volume	NOUN
american_scientific_journal-2520	45	80	27	27	NUM
american_scientific_journal-2520	45	81	,	,	PUNCT
american_scientific_journal-2520	45	82	no	no	DET
american_scientific_journal-2520	45	83	1	1	NUM
american_scientific_journal-2520	45	84	,	,	PUNCT
american_scientific_journal-2520	45	85	pp	pp	ADV
american_scientific_journal-2520	45	86	12	12	NUM
american_scientific_journal-2520	45	87	-	-	SYM
american_scientific_journal-2520	45	88	24	24	NUM
american_scientific_journal-2520	45	89	15	15	NUM
american_scientific_journal-2520	45	90	the	the	DET
american_scientific_journal-2520	45	91	first	first	ADJ
american_scientific_journal-2520	45	92	pcr	pcr	ADJ
american_scientific_journal-2520	45	93	product	product	NOUN
american_scientific_journal-2520	45	94	with	with	ADP
american_scientific_journal-2520	45	95	0.6µl	0.6µl	NUM
american_scientific_journal-2520	45	96	of	of	ADP
american_scientific_journal-2520	45	97	primer3	primer3	NOUN
american_scientific_journal-2520	45	98	and	and	CCONJ
american_scientific_journal-2520	45	99	1µl	1µl	NOUN
american_scientific_journal-2520	45	100	of	of	ADP
american_scientific_journal-2520	45	101	f	f	PROPN
american_scientific_journal-2520	45	102	primer	primer	NOUN
american_scientific_journal-2520	45	103	were	be	AUX
american_scientific_journal-2520	45	104	added	add	VERB
american_scientific_journal-2520	45	105	also	also	ADV
american_scientific_journal-2520	45	106	in	in	ADP
american_scientific_journal-2520	45	107	the	the	DET
american_scientific_journal-2520	45	108	third	third	ADJ
american_scientific_journal-2520	45	109	pcr	pcr	ADJ
american_scientific_journal-2520	45	110	cycle	cycle	NOUN
american_scientific_journal-2520	45	111	about	about	ADP
american_scientific_journal-2520	45	112	1µl	1µl	NOUN
american_scientific_journal-2520	45	113	of	of	ADP
american_scientific_journal-2520	45	114	the	the	DET
american_scientific_journal-2520	45	115	second	second	ADJ
american_scientific_journal-2520	45	116	pcr	pcr	ADJ
american_scientific_journal-2520	45	117	product	product	NOUN
american_scientific_journal-2520	45	118	with	with	ADP
american_scientific_journal-2520	45	119	0.6µl	0.6µl	NUM
american_scientific_journal-2520	45	120	of	of	ADP
american_scientific_journal-2520	45	121	primer	primer	NOUN
american_scientific_journal-2520	45	122	4	4	NUM
american_scientific_journal-2520	45	123	and	and	CCONJ
american_scientific_journal-2520	45	124	1µl	1µl	NOUN
american_scientific_journal-2520	45	125	of	of	ADP
american_scientific_journal-2520	45	126	f	f	PROPN
american_scientific_journal-2520	45	127	primer	primer	NOUN
american_scientific_journal-2520	45	128	were	be	AUX
american_scientific_journal-2520	45	129	added	add	VERB
american_scientific_journal-2520	45	130	.	.	PUNCT
american_scientific_journal-2520	46	1	finally	finally	ADV
american_scientific_journal-2520	46	2	,	,	PUNCT
american_scientific_journal-2520	46	3	in	in	ADP
american_scientific_journal-2520	46	4	the	the	DET
american_scientific_journal-2520	46	5	fourth	fourth	ADJ
american_scientific_journal-2520	46	6	pcr	pcr	ADJ
american_scientific_journal-2520	46	7	cycle	cycle	NOUN
american_scientific_journal-2520	46	8	about	about	ADP
american_scientific_journal-2520	46	9	1µl	1µl	NOUN
american_scientific_journal-2520	46	10	of	of	ADP
american_scientific_journal-2520	46	11	the	the	DET
american_scientific_journal-2520	46	12	third	third	ADJ
american_scientific_journal-2520	46	13	pcr	pcr	ADJ
american_scientific_journal-2520	46	14	product	product	NOUN
american_scientific_journal-2520	46	15	with	with	ADP
american_scientific_journal-2520	46	16	0.6µl	0.6µl	NUM
american_scientific_journal-2520	46	17	of	of	ADP
american_scientific_journal-2520	46	18	the	the	DET
american_scientific_journal-2520	46	19	fifth	fifth	ADJ
american_scientific_journal-2520	46	20	primer	primer	NOUN
american_scientific_journal-2520	46	21	and	and	CCONJ
american_scientific_journal-2520	46	22	1µl	1µl	NOUN
american_scientific_journal-2520	46	23	of	of	ADP
american_scientific_journal-2520	46	24	the	the	DET
american_scientific_journal-2520	46	25	f	f	PROPN
american_scientific_journal-2520	46	26	primer	primer	NOUN
american_scientific_journal-2520	46	27	.	.	PUNCT
american_scientific_journal-2520	47	1	the	the	DET
american_scientific_journal-2520	47	2	pcr	pcr	ADJ
american_scientific_journal-2520	47	3	reaction	reaction	NOUN
american_scientific_journal-2520	47	4	for	for	ADP
american_scientific_journal-2520	47	5	the	the	DET
american_scientific_journal-2520	47	6	above	above	ADJ
american_scientific_journal-2520	47	7	pcr	pcr	ADJ
american_scientific_journal-2520	47	8	cycles	cycle	NOUN
american_scientific_journal-2520	47	9	was	be	AUX
american_scientific_journal-2520	47	10	proceeding	proceed	VERB
american_scientific_journal-2520	47	11	to	to	ADP
american_scientific_journal-2520	47	12	thermal	thermal	ADJ
american_scientific_journal-2520	47	13	cycling	cycling	NOUN
american_scientific_journal-2520	47	14	with	with	ADP
american_scientific_journal-2520	47	15	the	the	DET
american_scientific_journal-2520	47	16	initial	initial	ADJ
american_scientific_journal-2520	47	17	denaturation	denaturation	NOUN
american_scientific_journal-2520	47	18	temp	temp	NOUN
american_scientific_journal-2520	47	19	.	.	PUNCT
american_scientific_journal-2520	48	1	95c	95c	NOUN
american_scientific_journal-2520	48	2	°	°	NUM
american_scientific_journal-2520	48	3	for	for	ADP
american_scientific_journal-2520	48	4	2	2	NUM
american_scientific_journal-2520	48	5	min	min	NOUN
american_scientific_journal-2520	48	6	and	and	CCONJ
american_scientific_journal-2520	48	7	30	30	NUM
american_scientific_journal-2520	48	8	cycles	cycle	NOUN
american_scientific_journal-2520	48	9	of	of	ADP
american_scientific_journal-2520	48	10	20sec	20sec	NOUN
american_scientific_journal-2520	48	11	.	.	PUNCT
american_scientific_journal-2520	49	1	at	at	ADP
american_scientific_journal-2520	49	2	95c	95c	NOUN
american_scientific_journal-2520	49	3	°	°	NOUN
american_scientific_journal-2520	49	4	,	,	PUNCT
american_scientific_journal-2520	49	5	20sec	20sec	NOUN
american_scientific_journal-2520	49	6	.	.	PUNCT
american_scientific_journal-2520	50	1	at	at	ADP
american_scientific_journal-2520	50	2	55c	55c	NUM
american_scientific_journal-2520	50	3	°	°	PROPN
american_scientific_journal-2520	50	4	,	,	PUNCT
american_scientific_journal-2520	50	5	40sec	40sec	NOUN
american_scientific_journal-2520	50	6	.	.	PUNCT
american_scientific_journal-2520	51	1	at72	at72	PROPN
american_scientific_journal-2520	51	2	c	c	PROPN
american_scientific_journal-2520	51	3	°	°	NUM
american_scientific_journal-2520	51	4	and	and	CCONJ
american_scientific_journal-2520	51	5	final	final	ADJ
american_scientific_journal-2520	51	6	extension	extension	NOUN
american_scientific_journal-2520	51	7	at	at	ADP
american_scientific_journal-2520	51	8	72c	72c	NOUN
american_scientific_journal-2520	51	9	°	°	NOUN
american_scientific_journal-2520	51	10	for	for	ADP
american_scientific_journal-2520	51	11	5min	5min	NOUN
american_scientific_journal-2520	51	12	.	.	PUNCT
american_scientific_journal-2520	52	1	one	one	NUM
american_scientific_journal-2520	52	2	time	time	NOUN
american_scientific_journal-2520	52	3	after	after	ADP
american_scientific_journal-2520	52	4	that	that	PRON
american_scientific_journal-2520	52	5	the	the	DET
american_scientific_journal-2520	52	6	pcr	pcr	ADJ
american_scientific_journal-2520	52	7	products	product	NOUN
american_scientific_journal-2520	52	8	loaded	load	VERB
american_scientific_journal-2520	52	9	in	in	ADP
american_scientific_journal-2520	52	10	the	the	DET
american_scientific_journal-2520	52	11	wells	well	NOUN
american_scientific_journal-2520	52	12	of	of	ADP
american_scientific_journal-2520	52	13	2	2	NUM
american_scientific_journal-2520	52	14	%	%	NOUN
american_scientific_journal-2520	52	15	agarose	agarose	NOUN
american_scientific_journal-2520	52	16	gel	gel	NOUN
american_scientific_journal-2520	52	17	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2520	52	18	.	.	PUNCT
american_scientific_journal-2520	53	1	2.2	2.2	NUM
american_scientific_journal-2520	53	2	.	.	PUNCT
american_scientific_journal-2520	53	3	purification	purification	NOUN
american_scientific_journal-2520	53	4	of	of	ADP
american_scientific_journal-2520	53	5	recombinant	recombinant	ADJ
american_scientific_journal-2520	53	6	shpth	shpth	ADJ
american_scientific_journal-2520	53	7	gene	gene	NOUN
american_scientific_journal-2520	53	8	after	after	ADP
american_scientific_journal-2520	53	9	detecting	detect	VERB
american_scientific_journal-2520	53	10	the	the	DET
american_scientific_journal-2520	53	11	desired	desire	VERB
american_scientific_journal-2520	53	12	shpth	shpth	ADJ
american_scientific_journal-2520	53	13	gene	gene	NOUN
american_scientific_journal-2520	53	14	by	by	ADP
american_scientific_journal-2520	53	15	running	run	VERB
american_scientific_journal-2520	53	16	it	it	PRON
american_scientific_journal-2520	53	17	in	in	ADP
american_scientific_journal-2520	53	18	agarose	agarose	NOUN
american_scientific_journal-2520	53	19	gel	gel	NOUN
american_scientific_journal-2520	53	20	the	the	DET
american_scientific_journal-2520	53	21	gene	gene	NOUN
american_scientific_journal-2520	53	22	was	be	AUX
american_scientific_journal-2520	53	23	purified	purify	VERB
american_scientific_journal-2520	53	24	from	from	ADP
american_scientific_journal-2520	53	25	agarose	agarose	NOUN
american_scientific_journal-2520	53	26	gel	gel	NOUN
american_scientific_journal-2520	53	27	by	by	ADP
american_scientific_journal-2520	53	28	using	use	VERB
american_scientific_journal-2520	53	29	mega	mega	ADJ
american_scientific_journal-2520	53	30	quick	quick	ADJ
american_scientific_journal-2520	53	31	-	-	PUNCT
american_scientific_journal-2520	53	32	spin	spin	NOUN
american_scientific_journal-2520	53	33	™	™	ADJ
american_scientific_journal-2520	53	34	total	total	ADJ
american_scientific_journal-2520	53	35	fragment	fragment	ADJ
american_scientific_journal-2520	53	36	dna	dna	NOUN
american_scientific_journal-2520	53	37	purification	purification	NOUN
american_scientific_journal-2520	53	38	kit	kit	NOUN
american_scientific_journal-2520	53	39	from	from	ADP
american_scientific_journal-2520	53	40	(	(	PUNCT
american_scientific_journal-2520	53	41	intron	intron	PROPN
american_scientific_journal-2520	53	42	)	)	PUNCT
american_scientific_journal-2520	53	43	korea	korea	PROPN
american_scientific_journal-2520	53	44	.	.	PUNCT
american_scientific_journal-2520	54	1	and	and	CCONJ
american_scientific_journal-2520	54	2	the	the	DET
american_scientific_journal-2520	54	3	concentration	concentration	NOUN
american_scientific_journal-2520	54	4	of	of	ADP
american_scientific_journal-2520	54	5	the	the	DET
american_scientific_journal-2520	54	6	dsdna	dsdna	NOUN
american_scientific_journal-2520	54	7	for	for	ADP
american_scientific_journal-2520	54	8	purified	purified	ADJ
american_scientific_journal-2520	54	9	shpth	shpth	ADJ
american_scientific_journal-2520	54	10	gene	gene	NOUN
american_scientific_journal-2520	54	11	measured	measure	VERB
american_scientific_journal-2520	54	12	by	by	ADP
american_scientific_journal-2520	54	13	using	use	VERB
american_scientific_journal-2520	54	14	the	the	DET
american_scientific_journal-2520	54	15	optizen	optizen	ADJ
american_scientific_journal-2520	54	16	pop	pop	NOUN
american_scientific_journal-2520	54	17	nano	nano	NOUN
american_scientific_journal-2520	54	18	bio	bio	NOUN
american_scientific_journal-2520	54	19	spectrophotometer	spectrophotometer	NOUN
american_scientific_journal-2520	54	20	.	.	PUNCT
american_scientific_journal-2520	55	1	2.3	2.3	NUM
american_scientific_journal-2520	55	2	.	.	PUNCT
american_scientific_journal-2520	56	1	construction	construction	NOUN
american_scientific_journal-2520	56	2	of	of	ADP
american_scientific_journal-2520	56	3	recombinant	recombinant	ADJ
american_scientific_journal-2520	56	4	pet32a	pet32a	NOUN
american_scientific_journal-2520	56	5	-	-	NOUN
american_scientific_journal-2520	56	6	hpth	hpth	ADJ
american_scientific_journal-2520	56	7	vector	vector	NOUN
american_scientific_journal-2520	56	8	the	the	DET
american_scientific_journal-2520	56	9	pet	pet	ADJ
american_scientific_journal-2520	56	10	32a	32a	NOUN
american_scientific_journal-2520	56	11	(	(	PUNCT
american_scientific_journal-2520	56	12	+	+	ADJ
american_scientific_journal-2520	56	13	)	)	PUNCT
american_scientific_journal-2520	56	14	vector	vector	NOUN
american_scientific_journal-2520	56	15	cat	cat	NOUN
american_scientific_journal-2520	56	16	.	.	PUNCT
american_scientific_journal-2520	57	1	no.69015	no.69015	PROPN
american_scientific_journal-2520	57	2	was	be	AUX
american_scientific_journal-2520	57	3	used	use	VERB
american_scientific_journal-2520	57	4	in	in	ADP
american_scientific_journal-2520	57	5	this	this	DET
american_scientific_journal-2520	57	6	study	study	NOUN
american_scientific_journal-2520	57	7	,	,	PUNCT
american_scientific_journal-2520	57	8	the	the	DET
american_scientific_journal-2520	57	9	constructing	construct	VERB
american_scientific_journal-2520	57	10	pet	pet	ADJ
american_scientific_journal-2520	57	11	32a	32a	NOUN
american_scientific_journal-2520	57	12	(	(	PUNCT
american_scientific_journal-2520	57	13	+	+	NOUN
american_scientific_journal-2520	57	14	)	)	PUNCT
american_scientific_journal-2520	57	15	hpth	hpth	NOUN
american_scientific_journal-2520	57	16	can	can	AUX
american_scientific_journal-2520	57	17	be	be	AUX
american_scientific_journal-2520	57	18	achieved	achieve	VERB
american_scientific_journal-2520	57	19	by	by	ADP
american_scientific_journal-2520	57	20	digestion	digestion	NOUN
american_scientific_journal-2520	57	21	of	of	ADP
american_scientific_journal-2520	57	22	both	both	CCONJ
american_scientific_journal-2520	57	23	the	the	DET
american_scientific_journal-2520	57	24	shpth	shpth	ADJ
american_scientific_journal-2520	57	25	gene	gene	NOUN
american_scientific_journal-2520	57	26	and	and	CCONJ
american_scientific_journal-2520	57	27	the	the	DET
american_scientific_journal-2520	57	28	pet	pet	ADJ
american_scientific_journal-2520	57	29	32a	32a	NOUN
american_scientific_journal-2520	57	30	(	(	PUNCT
american_scientific_journal-2520	57	31	+	+	NOUN
american_scientific_journal-2520	57	32	)	)	PUNCT
american_scientific_journal-2520	57	33	plasmid	plasmid	NOUN
american_scientific_journal-2520	57	34	by	by	ADP
american_scientific_journal-2520	57	35	endonuclease	endonuclease	NOUN
american_scientific_journal-2520	57	36	restriction	restriction	NOUN
american_scientific_journal-2520	57	37	enzymes	enzyme	NOUN
american_scientific_journal-2520	57	38	primarlly	primarlly	ADV
american_scientific_journal-2520	57	39	by	by	ADP
american_scientific_journal-2520	57	40	hindiii	hindiii	ADJ
american_scientific_journal-2520	57	41	cat	cat	NOUN
american_scientific_journal-2520	57	42	.	.	PUNCT
american_scientific_journal-2520	58	1	no	no	INTJ
american_scientific_journal-2520	58	2	.	.	PUNCT
american_scientific_journal-2520	59	1	r6041	r6041	NOUN
american_scientific_journal-2520	59	2	and	and	CCONJ
american_scientific_journal-2520	59	3	then	then	ADV
american_scientific_journal-2520	59	4	by	by	ADP
american_scientific_journal-2520	59	5	kpni	kpni	ADJ
american_scientific_journal-2520	59	6	cat	cat	NOUN
american_scientific_journal-2520	59	7	.	.	PUNCT
american_scientific_journal-2520	60	1	no.15232010	no.15232010	PROPN
american_scientific_journal-2520	60	2	,	,	PUNCT
american_scientific_journal-2520	60	3	the	the	DET
american_scientific_journal-2520	60	4	procedure	procedure	NOUN
american_scientific_journal-2520	60	5	of	of	ADP
american_scientific_journal-2520	60	6	restriction	restriction	NOUN
american_scientific_journal-2520	60	7	reactions	reaction	NOUN
american_scientific_journal-2520	60	8	was	be	AUX
american_scientific_journal-2520	60	9	prepared	prepare	VERB
american_scientific_journal-2520	60	10	according	accord	VERB
american_scientific_journal-2520	60	11	to	to	ADP
american_scientific_journal-2520	60	12	the	the	DET
american_scientific_journal-2520	60	13	protocols	protocol	NOUN
american_scientific_journal-2520	60	14	of	of	ADP
american_scientific_journal-2520	60	15	supplied	supply	VERB
american_scientific_journal-2520	60	16	companies	company	NOUN
american_scientific_journal-2520	60	17	.	.	PUNCT
american_scientific_journal-2520	61	1	ligation	ligation	NOUN
american_scientific_journal-2520	61	2	of	of	ADP
american_scientific_journal-2520	61	3	the	the	DET
american_scientific_journal-2520	61	4	restricted	restricted	ADJ
american_scientific_journal-2520	61	5	shpth	shpth	ADJ
american_scientific_journal-2520	61	6	gene	gene	NOUN
american_scientific_journal-2520	61	7	and	and	CCONJ
american_scientific_journal-2520	61	8	restricted	restrict	VERB
american_scientific_journal-2520	61	9	pet	pet	ADJ
american_scientific_journal-2520	61	10	32a	32a	NOUN
american_scientific_journal-2520	61	11	plasmid	plasmid	NOUN
american_scientific_journal-2520	61	12	was	be	AUX
american_scientific_journal-2520	61	13	achieved	achieve	VERB
american_scientific_journal-2520	61	14	by	by	ADP
american_scientific_journal-2520	61	15	using	use	VERB
american_scientific_journal-2520	61	16	the	the	DET
american_scientific_journal-2520	61	17	accupower	accupower	NOUN
american_scientific_journal-2520	61	18	®	®	NOUN
american_scientific_journal-2520	61	19	ligation	ligation	NOUN
american_scientific_journal-2520	61	20	premix	premix	NOUN
american_scientific_journal-2520	61	21	cat	cat	NOUN
american_scientific_journal-2520	61	22	.	.	PUNCT
american_scientific_journal-2520	62	1	no	no	INTJ
american_scientific_journal-2520	62	2	.	.	PUNCT
american_scientific_journal-2520	63	1	k-7103	k-7103	NOUN
american_scientific_journal-2520	63	2	,	,	PUNCT
american_scientific_journal-2520	63	3	and	and	CCONJ
american_scientific_journal-2520	63	4	as	as	ADP
american_scientific_journal-2520	63	5	the	the	DET
american_scientific_journal-2520	63	6	protocol	protocol	NOUN
american_scientific_journal-2520	63	7	provided	provide	VERB
american_scientific_journal-2520	63	8	with	with	ADP
american_scientific_journal-2520	63	9	the	the	DET
american_scientific_journal-2520	63	10	ligation	ligation	NOUN
american_scientific_journal-2520	63	11	premix	premix	NOUN
american_scientific_journal-2520	63	12	.	.	PUNCT
american_scientific_journal-2520	64	1	2.4	2.4	NUM
american_scientific_journal-2520	64	2	.	.	PUNCT
american_scientific_journal-2520	65	1	transformation	transformation	NOUN
american_scientific_journal-2520	65	2	of	of	ADP
american_scientific_journal-2520	65	3	competent	competent	ADJ
american_scientific_journal-2520	65	4	cells	cell	NOUN
american_scientific_journal-2520	65	5	the	the	DET
american_scientific_journal-2520	65	6	constructed	construct	VERB
american_scientific_journal-2520	65	7	vector	vector	NOUN
american_scientific_journal-2520	65	8	pet32a	pet32a	NOUN
american_scientific_journal-2520	65	9	-	-	ADJ
american_scientific_journal-2520	65	10	shpth	shpth	ADJ
american_scientific_journal-2520	65	11	was	be	AUX
american_scientific_journal-2520	65	12	transformed	transform	VERB
american_scientific_journal-2520	65	13	into	into	ADP
american_scientific_journal-2520	65	14	two	two	NUM
american_scientific_journal-2520	65	15	genetic	genetic	ADJ
american_scientific_journal-2520	65	16	engineered	engineer	VERB
american_scientific_journal-2520	65	17	e.	e.	PROPN
american_scientific_journal-2520	65	18	coli	coli	PROPN
american_scientific_journal-2520	65	19	strains	strain	VERB
american_scientific_journal-2520	65	20	primarily	primarily	ADV
american_scientific_journal-2520	65	21	in	in	ADP
american_scientific_journal-2520	65	22	to	to	ADP
american_scientific_journal-2520	65	23	dh5α	dh5α	PROPN
american_scientific_journal-2520	65	24	strain	strain	NOUN
american_scientific_journal-2520	65	25	and	and	CCONJ
american_scientific_journal-2520	65	26	then	then	ADV
american_scientific_journal-2520	65	27	transformed	transform	VERB
american_scientific_journal-2520	65	28	into	into	ADP
american_scientific_journal-2520	65	29	the	the	DET
american_scientific_journal-2520	65	30	bl21(de3	bl21(de3	NOUN
american_scientific_journal-2520	65	31	)	)	PUNCT
american_scientific_journal-2520	65	32	strain	strain	NOUN
american_scientific_journal-2520	65	33	,	,	PUNCT
american_scientific_journal-2520	65	34	the	the	DET
american_scientific_journal-2520	65	35	transformation	transformation	NOUN
american_scientific_journal-2520	65	36	methods	method	NOUN
american_scientific_journal-2520	65	37	were	be	AUX
american_scientific_journal-2520	65	38	done	do	VERB
american_scientific_journal-2520	65	39	according	accord	VERB
american_scientific_journal-2520	65	40	to	to	ADP
american_scientific_journal-2520	65	41	the	the	DET
american_scientific_journal-2520	65	42	protocols	protocol	NOUN
american_scientific_journal-2520	65	43	provided	provide	VERB
american_scientific_journal-2520	65	44	with	with	ADP
american_scientific_journal-2520	65	45	the	the	DET
american_scientific_journal-2520	65	46	novagen	novagen	PROPN
american_scientific_journal-2520	65	47	and	and	CCONJ
american_scientific_journal-2520	65	48	invitrogen	invitrogen	PROPN
american_scientific_journal-2520	65	49	companies	company	NOUN
american_scientific_journal-2520	65	50	.	.	PUNCT
american_scientific_journal-2520	66	1	2.5	2.5	NUM
american_scientific_journal-2520	66	2	.	.	PUNCT
american_scientific_journal-2520	67	1	screening	screen	VERB
american_scientific_journal-2520	67	2	for	for	ADP
american_scientific_journal-2520	67	3	cloning	clone	VERB
american_scientific_journal-2520	67	4	isolates	isolate	NOUN
american_scientific_journal-2520	67	5	to	to	PART
american_scientific_journal-2520	67	6	determine	determine	VERB
american_scientific_journal-2520	67	7	the	the	DET
american_scientific_journal-2520	67	8	presence	presence	NOUN
american_scientific_journal-2520	67	9	of	of	ADP
american_scientific_journal-2520	67	10	target	target	NOUN
american_scientific_journal-2520	67	11	gene	gene	NOUN
american_scientific_journal-2520	67	12	in	in	ADP
american_scientific_journal-2520	67	13	the	the	DET
american_scientific_journal-2520	67	14	transformed	transform	VERB
american_scientific_journal-2520	67	15	competent	competent	ADJ
american_scientific_journal-2520	67	16	e.	e.	PROPN
american_scientific_journal-2520	67	17	coli	coli	PROPN
american_scientific_journal-2520	67	18	strains	strain	NOUN
american_scientific_journal-2520	67	19	(	(	PUNCT
american_scientific_journal-2520	67	20	which	which	PRON
american_scientific_journal-2520	67	21	growing	grow	VERB
american_scientific_journal-2520	67	22	after	after	ADP
american_scientific_journal-2520	67	23	transformation	transformation	NOUN
american_scientific_journal-2520	67	24	on	on	ADP
american_scientific_journal-2520	67	25	the	the	DET
american_scientific_journal-2520	67	26	selective	selective	ADJ
american_scientific_journal-2520	67	27	lb	lb	NOUN
american_scientific_journal-2520	67	28	plate	plate	NOUN
american_scientific_journal-2520	67	29	with	with	ADP
american_scientific_journal-2520	67	30	ampicillin	ampicillin	NOUN
american_scientific_journal-2520	67	31	)	)	PUNCT
american_scientific_journal-2520	67	32	,	,	PUNCT
american_scientific_journal-2520	67	33	a	a	DET
american_scientific_journal-2520	67	34	single	single	ADJ
american_scientific_journal-2520	67	35	colony	colony	NOUN
american_scientific_journal-2520	67	36	was	be	AUX
american_scientific_journal-2520	67	37	cultured	cultured	ADJ
american_scientific_journal-2520	67	38	in	in	ADP
american_scientific_journal-2520	67	39	10	10	NUM
american_scientific_journal-2520	67	40	ml	ml	NOUN
american_scientific_journal-2520	67	41	lb	lb	PRON
american_scientific_journal-2520	67	42	broth	broth	NOUN
american_scientific_journal-2520	67	43	containing	contain	VERB
american_scientific_journal-2520	67	44	ampicillin	ampicillin	NOUN
american_scientific_journal-2520	67	45	(	(	PUNCT
american_scientific_journal-2520	67	46	50	50	NUM
american_scientific_journal-2520	67	47	μg	μg	NOUN
american_scientific_journal-2520	67	48	/	/	SYM
american_scientific_journal-2520	67	49	ml	ml	NOUN
american_scientific_journal-2520	67	50	)	)	PUNCT
american_scientific_journal-2520	67	51	overnight	overnight	ADV
american_scientific_journal-2520	67	52	at	at	ADP
american_scientific_journal-2520	67	53	37c	37c	NUM
american_scientific_journal-2520	67	54	°	°	NOUN
american_scientific_journal-2520	67	55	,	,	PUNCT
american_scientific_journal-2520	67	56	then	then	ADV
american_scientific_journal-2520	67	57	from	from	ADP
american_scientific_journal-2520	67	58	2	2	NUM
american_scientific_journal-2520	67	59	to	to	ADP
american_scientific_journal-2520	67	60	5ml	5ml	NOUN
american_scientific_journal-2520	67	61	of	of	ADP
american_scientific_journal-2520	67	62	the	the	DET
american_scientific_journal-2520	67	63	bacterial	bacterial	ADJ
american_scientific_journal-2520	67	64	broth	broth	NOUN
american_scientific_journal-2520	67	65	was	be	AUX
american_scientific_journal-2520	67	66	used	use	VERB
american_scientific_journal-2520	67	67	for	for	ADP
american_scientific_journal-2520	67	68	plasmid	plasmid	ADJ
american_scientific_journal-2520	67	69	extraction	extraction	NOUN
american_scientific_journal-2520	67	70	by	by	ADP
american_scientific_journal-2520	67	71	accuprep	accuprep	NOUN
american_scientific_journal-2520	67	72	®	®	NOUN
american_scientific_journal-2520	67	73	plasmid	plasmid	NOUN
american_scientific_journal-2520	67	74	mini	mini	ADJ
american_scientific_journal-2520	67	75	extraction	extraction	NOUN
american_scientific_journal-2520	67	76	kit	kit	NOUN
american_scientific_journal-2520	67	77	cat	cat	NOUN
american_scientific_journal-2520	67	78	.	.	PUNCT
american_scientific_journal-2520	68	1	no	no	INTJ
american_scientific_journal-2520	68	2	.	.	PUNCT
american_scientific_journal-2520	68	3	:	:	PUNCT
american_scientific_journal-2520	69	1	k-3030	k-3030	PROPN
american_scientific_journal-2520	69	2	.	.	PUNCT
american_scientific_journal-2520	70	1	the	the	DET
american_scientific_journal-2520	70	2	target	target	NOUN
american_scientific_journal-2520	70	3	gene	gene	NOUN
american_scientific_journal-2520	70	4	shpth	shpth	NOUN
american_scientific_journal-2520	70	5	which	which	PRON
american_scientific_journal-2520	70	6	is	be	AUX
american_scientific_journal-2520	70	7	found	find	VERB
american_scientific_journal-2520	70	8	in	in	ADP
american_scientific_journal-2520	70	9	the	the	DET
american_scientific_journal-2520	70	10	pet32	pet32	PROPN
american_scientific_journal-2520	70	11	a	a	DET
american_scientific_journal-2520	70	12	(	(	PUNCT
american_scientific_journal-2520	70	13	+	+	NOUN
american_scientific_journal-2520	70	14	)	)	PUNCT
american_scientific_journal-2520	70	15	-hpth	-hpth	NOUN
american_scientific_journal-2520	70	16	(	(	PUNCT
american_scientific_journal-2520	70	17	extracted	extract	VERB
american_scientific_journal-2520	70	18	plasmid	plasmid	NOUN
american_scientific_journal-2520	70	19	)	)	PUNCT
american_scientific_journal-2520	70	20	amplified	amplify	VERB
american_scientific_journal-2520	70	21	by	by	ADP
american_scientific_journal-2520	70	22	using	use	VERB
american_scientific_journal-2520	70	23	whole	whole	ADJ
american_scientific_journal-2520	70	24	gene	gene	NOUN
american_scientific_journal-2520	70	25	amplified	amplify	VERB
american_scientific_journal-2520	70	26	(	(	PUNCT
american_scientific_journal-2520	70	27	f	f	NOUN
american_scientific_journal-2520	70	28	and	and	CCONJ
american_scientific_journal-2520	70	29	r	r	NOUN
american_scientific_journal-2520	70	30	primers	primer	NOUN
american_scientific_journal-2520	70	31	)	)	PUNCT
american_scientific_journal-2520	70	32	,	,	PUNCT
american_scientific_journal-2520	70	33	and	and	CCONJ
american_scientific_journal-2520	70	34	the	the	DET
american_scientific_journal-2520	70	35	pcr	pcr	ADJ
american_scientific_journal-2520	70	36	tube	tube	NOUN
american_scientific_journal-2520	70	37	contain	contain	VERB
american_scientific_journal-2520	70	38	12.5µl	12.5µl	NUM
american_scientific_journal-2520	70	39	of	of	ADP
american_scientific_journal-2520	70	40	gotaq	gotaq	PROPN
american_scientific_journal-2520	70	41	®	®	NOUN
american_scientific_journal-2520	70	42	green	green	ADJ
american_scientific_journal-2520	70	43	master	master	PROPN
american_scientific_journal-2520	70	44	mix	mix	PROPN
american_scientific_journal-2520	70	45	,	,	PUNCT
american_scientific_journal-2520	70	46	1	1	NUM
american_scientific_journal-2520	70	47	µl	µl	ADP
american_scientific_journal-2520	70	48	of	of	ADP
american_scientific_journal-2520	70	49	each	each	DET
american_scientific_journal-2520	70	50	primer	primer	NOUN
american_scientific_journal-2520	70	51	,	,	PUNCT
american_scientific_journal-2520	70	52	3.5µl	3.5µl	NUM
american_scientific_journal-2520	70	53	of	of	ADP
american_scientific_journal-2520	70	54	extracted	extract	VERB
american_scientific_journal-2520	70	55	plasmid	plasmid	NOUN
american_scientific_journal-2520	70	56	and	and	CCONJ
american_scientific_journal-2520	70	57	complete	complete	VERB
american_scientific_journal-2520	70	58	the	the	DET
american_scientific_journal-2520	70	59	volume	volume	NOUN
american_scientific_journal-2520	70	60	to	to	ADP
american_scientific_journal-2520	70	61	25	25	NUM
american_scientific_journal-2520	70	62	µl	µl	NOUN
american_scientific_journal-2520	70	63	with	with	ADP
american_scientific_journal-2520	70	64	nuclease	nuclease	NOUN
american_scientific_journal-2520	70	65	free	free	ADJ
american_scientific_journal-2520	70	66	water	water	NOUN
american_scientific_journal-2520	70	67	.	.	PUNCT
american_scientific_journal-2520	71	1	the	the	DET
american_scientific_journal-2520	71	2	thermal	thermal	ADJ
american_scientific_journal-2520	71	3	cycler	cycler	NOUN
american_scientific_journal-2520	71	4	program	program	NOUN
american_scientific_journal-2520	71	5	was	be	AUX
american_scientific_journal-2520	71	6	2	2	NUM
american_scientific_journal-2520	71	7	min	min	NOUN
american_scientific_journal-2520	71	8	at	at	ADP
american_scientific_journal-2520	71	9	95c	95c	NOUN
american_scientific_journal-2520	71	10	°	°	ADP
american_scientific_journal-2520	71	11	one	one	NUM
american_scientific_journal-2520	71	12	time	time	NOUN
american_scientific_journal-2520	71	13	,	,	PUNCT
american_scientific_journal-2520	71	14	30	30	NUM
american_scientific_journal-2520	71	15	cycles	cycle	NOUN
american_scientific_journal-2520	71	16	of	of	ADP
american_scientific_journal-2520	71	17	30sec	30sec	NOUN
american_scientific_journal-2520	71	18	.	.	PUNCT
american_scientific_journal-2520	72	1	at	at	ADP
american_scientific_journal-2520	72	2	95c	95c	NOUN
american_scientific_journal-2520	72	3	°	°	NOUN
american_scientific_journal-2520	72	4	,	,	PUNCT
american_scientific_journal-2520	72	5	30	30	NUM
american_scientific_journal-2520	72	6	sec	sec	NOUN
american_scientific_journal-2520	72	7	.	.	PUNCT
american_scientific_journal-2520	73	1	at	at	ADP
american_scientific_journal-2520	73	2	57.8c	57.8c	NUM
american_scientific_journal-2520	73	3	°	°	NOUN
american_scientific_journal-2520	73	4	,	,	PUNCT
american_scientific_journal-2520	73	5	40	40	NUM
american_scientific_journal-2520	73	6	sec	sec	NOUN
american_scientific_journal-2520	73	7	.	.	PUNCT
american_scientific_journal-2520	74	1	at	at	ADP
american_scientific_journal-2520	74	2	72	72	NUM
american_scientific_journal-2520	74	3	c	c	NOUN
american_scientific_journal-2520	74	4	°	°	NUM
american_scientific_journal-2520	74	5	and	and	CCONJ
american_scientific_journal-2520	74	6	final	final	ADJ
american_scientific_journal-2520	74	7	extension	extension	NOUN
american_scientific_journal-2520	74	8	at	at	ADP
american_scientific_journal-2520	74	9	72c	72c	NOUN
american_scientific_journal-2520	74	10	°	°	NOUN
american_scientific_journal-2520	74	11	for	for	ADP
american_scientific_journal-2520	74	12	5min	5min	NOUN
american_scientific_journal-2520	74	13	.	.	PUNCT
american_scientific_journal-2520	74	14	2.6	2.6	NUM
american_scientific_journal-2520	74	15	.	.	PUNCT
american_scientific_journal-2520	74	16	testing	test	VERB
american_scientific_journal-2520	74	17	for	for	ADP
american_scientific_journal-2520	74	18	cloning	clone	VERB
american_scientific_journal-2520	74	19	shpth	shpth	ADJ
american_scientific_journal-2520	74	20	gene	gene	NOUN
american_scientific_journal-2520	74	21	expression	expression	NOUN
american_scientific_journal-2520	74	22	by	by	ADP
american_scientific_journal-2520	74	23	real	real	ADJ
american_scientific_journal-2520	74	24	time	time	NOUN
american_scientific_journal-2520	74	25	pcr	pcr	PROPN
american_scientific_journal-2520	74	26	http://eng.bioneer.com/pdf/09_eng/accupowerligation_eng.pdf	http://eng.bioneer.com/pdf/09_eng/accupowerligation_eng.pdf	PROPN
american_scientific_journal-2520	74	27	american	american	PROPN
american_scientific_journal-2520	74	28	scientific	scientific	PROPN
american_scientific_journal-2520	74	29	research	research	PROPN
american_scientific_journal-2520	74	30	journal	journal	NOUN
american_scientific_journal-2520	74	31	for	for	ADP
american_scientific_journal-2520	74	32	engineering	engineering	NOUN
american_scientific_journal-2520	74	33	,	,	PUNCT
american_scientific_journal-2520	74	34	technology	technology	NOUN
american_scientific_journal-2520	74	35	,	,	PUNCT
american_scientific_journal-2520	74	36	and	and	CCONJ
american_scientific_journal-2520	74	37	sciences	science	NOUN
american_scientific_journal-2520	74	38	(	(	PUNCT
american_scientific_journal-2520	74	39	asrjets	asrjet	NOUN
american_scientific_journal-2520	74	40	)	)	PUNCT
american_scientific_journal-2520	74	41	(	(	PUNCT
american_scientific_journal-2520	74	42	2017	2017	NUM
american_scientific_journal-2520	74	43	)	)	PUNCT
american_scientific_journal-2520	74	44	volume	volume	NOUN
american_scientific_journal-2520	74	45	27	27	NUM
american_scientific_journal-2520	74	46	,	,	PUNCT
american_scientific_journal-2520	74	47	no	no	DET
american_scientific_journal-2520	74	48	1	1	NUM
american_scientific_journal-2520	74	49	,	,	PUNCT
american_scientific_journal-2520	74	50	pp	pp	ADV
american_scientific_journal-2520	74	51	12	12	NUM
american_scientific_journal-2520	74	52	-	-	SYM
american_scientific_journal-2520	74	53	24	24	NUM
american_scientific_journal-2520	74	54	16	16	NUM
american_scientific_journal-2520	74	55	a	a	DET
american_scientific_journal-2520	74	56	real	real	ADJ
american_scientific_journal-2520	74	57	-	-	PUNCT
american_scientific_journal-2520	74	58	time	time	NOUN
american_scientific_journal-2520	74	59	pcr	pcr	NOUN
american_scientific_journal-2520	74	60	is	be	AUX
american_scientific_journal-2520	74	61	a	a	DET
american_scientific_journal-2520	74	62	laboratory	laboratory	NOUN
american_scientific_journal-2520	74	63	technique	technique	NOUN
american_scientific_journal-2520	74	64	of	of	ADP
american_scientific_journal-2520	74	65	molecular	molecular	ADJ
american_scientific_journal-2520	74	66	biology	biology	NOUN
american_scientific_journal-2520	74	67	based	base	VERB
american_scientific_journal-2520	74	68	on	on	ADP
american_scientific_journal-2520	74	69	the	the	DET
american_scientific_journal-2520	74	70	(	(	PUNCT
american_scientific_journal-2520	74	71	pcr	pcr	NOUN
american_scientific_journal-2520	74	72	)	)	PUNCT
american_scientific_journal-2520	74	73	.	.	PUNCT
american_scientific_journal-2520	75	1	it	it	PRON
american_scientific_journal-2520	75	2	monitors	monitor	VERB
american_scientific_journal-2520	75	3	the	the	DET
american_scientific_journal-2520	75	4	amplification	amplification	NOUN
american_scientific_journal-2520	75	5	of	of	ADP
american_scientific_journal-2520	75	6	a	a	DET
american_scientific_journal-2520	75	7	targeted	target	VERB
american_scientific_journal-2520	75	8	dna	dna	NOUN
american_scientific_journal-2520	75	9	molecule	molecule	NOUN
american_scientific_journal-2520	75	10	during	during	ADP
american_scientific_journal-2520	75	11	the	the	DET
american_scientific_journal-2520	75	12	pcr	pcr	NOUN
american_scientific_journal-2520	75	13	,	,	PUNCT
american_scientific_journal-2520	75	14	in	in	ADP
american_scientific_journal-2520	75	15	this	this	DET
american_scientific_journal-2520	75	16	test	test	NOUN
american_scientific_journal-2520	75	17	the	the	DET
american_scientific_journal-2520	75	18	real	real	ADJ
american_scientific_journal-2520	75	19	-	-	PUNCT
american_scientific_journal-2520	75	20	time	time	NOUN
american_scientific_journal-2520	75	21	pcr	pcr	NOUN
american_scientific_journal-2520	75	22	used	use	VERB
american_scientific_journal-2520	75	23	as	as	ADP
american_scientific_journal-2520	75	24	gene	gene	NOUN
american_scientific_journal-2520	75	25	expression	expression	NOUN
american_scientific_journal-2520	75	26	analysis	analysis	NOUN
american_scientific_journal-2520	75	27	techniques	technique	NOUN
american_scientific_journal-2520	75	28	and	and	CCONJ
american_scientific_journal-2520	75	29	that	that	PRON
american_scientific_journal-2520	75	30	is	be	AUX
american_scientific_journal-2520	75	31	carried	carry	VERB
american_scientific_journal-2520	75	32	out	out	ADP
american_scientific_journal-2520	75	33	by	by	ADP
american_scientific_journal-2520	75	34	multiple	multiple	ADJ
american_scientific_journal-2520	75	35	steps	step	NOUN
american_scientific_journal-2520	75	36	:	:	PUNCT
american_scientific_journal-2520	75	37	first	first	ADJ
american_scientific_journal-2520	75	38	step	step	NOUN
american_scientific_journal-2520	75	39	:	:	PUNCT
american_scientific_journal-2520	75	40	isolation	isolation	NOUN
american_scientific_journal-2520	75	41	of	of	ADP
american_scientific_journal-2520	75	42	rna	rna	NOUN
american_scientific_journal-2520	75	43	,	,	PUNCT
american_scientific_journal-2520	75	44	the	the	DET
american_scientific_journal-2520	75	45	rna	rna	NOUN
american_scientific_journal-2520	75	46	of	of	ADP
american_scientific_journal-2520	75	47	cloning	clone	VERB
american_scientific_journal-2520	75	48	bl21	bl21	PROPN
american_scientific_journal-2520	75	49	(	(	PUNCT
american_scientific_journal-2520	75	50	de3	de3	NOUN
american_scientific_journal-2520	75	51	)	)	PUNCT
american_scientific_journal-2520	75	52	strain	strain	NOUN
american_scientific_journal-2520	75	53	was	be	AUX
american_scientific_journal-2520	75	54	extracted	extract	VERB
american_scientific_journal-2520	75	55	by	by	ADP
american_scientific_journal-2520	75	56	taking	take	VERB
american_scientific_journal-2520	75	57	2ml	2ml	NOUN
american_scientific_journal-2520	75	58	of	of	ADP
american_scientific_journal-2520	75	59	bacterial	bacterial	ADJ
american_scientific_journal-2520	75	60	broth	broth	NOUN
american_scientific_journal-2520	75	61	after	after	ADP
american_scientific_journal-2520	75	62	induction	induction	NOUN
american_scientific_journal-2520	75	63	by	by	ADP
american_scientific_journal-2520	75	64	adding	add	VERB
american_scientific_journal-2520	75	65	(	(	PUNCT
american_scientific_journal-2520	75	66	1	1	NUM
american_scientific_journal-2520	75	67	mm	mm	NOUN
american_scientific_journal-2520	75	68	)	)	PUNCT
american_scientific_journal-2520	75	69	of	of	ADP
american_scientific_journal-2520	75	70	iptg	iptg	NOUN
american_scientific_journal-2520	75	71	as	as	ADP
american_scientific_journal-2520	75	72	a	a	DET
american_scientific_journal-2520	75	73	final	final	ADJ
american_scientific_journal-2520	75	74	concentration	concentration	NOUN
american_scientific_journal-2520	75	75	to	to	ADP
american_scientific_journal-2520	75	76	the	the	DET
american_scientific_journal-2520	75	77	lb	lb	DET
american_scientific_journal-2520	75	78	broth	broth	NOUN
american_scientific_journal-2520	75	79	containing	contain	VERB
american_scientific_journal-2520	75	80	the	the	DET
american_scientific_journal-2520	75	81	transformed	transform	VERB
american_scientific_journal-2520	75	82	bacteria	bacteria	NOUN
american_scientific_journal-2520	75	83	and	and	CCONJ
american_scientific_journal-2520	75	84	then	then	ADV
american_scientific_journal-2520	75	85	the	the	DET
american_scientific_journal-2520	75	86	bacteria	bacteria	NOUN
american_scientific_journal-2520	75	87	was	be	AUX
american_scientific_journal-2520	75	88	grown	grow	VERB
american_scientific_journal-2520	75	89	with	with	ADP
american_scientific_journal-2520	75	90	shaking	shake	VERB
american_scientific_journal-2520	75	91	for	for	ADP
american_scientific_journal-2520	75	92	4	4	NUM
american_scientific_journal-2520	75	93	an	an	DET
american_scientific_journal-2520	75	94	hours	hour	NOUN
american_scientific_journal-2520	75	95	at	at	ADP
american_scientific_journal-2520	75	96	30c°.the	30c°.the	PROPN
american_scientific_journal-2520	75	97	rna	rna	NOUN
american_scientific_journal-2520	75	98	extraction	extraction	NOUN
american_scientific_journal-2520	75	99	was	be	AUX
american_scientific_journal-2520	75	100	carried	carry	VERB
american_scientific_journal-2520	75	101	out	out	ADP
american_scientific_journal-2520	75	102	manually	manually	ADV
american_scientific_journal-2520	75	103	by	by	ADP
american_scientific_journal-2520	75	104	using	use	VERB
american_scientific_journal-2520	75	105	accuzoltm	accuzoltm	NOUN
american_scientific_journal-2520	75	106	cat	cat	PROPN
american_scientific_journal-2520	75	107	no	no	INTJ
american_scientific_journal-2520	75	108	.	.	PUNCT
american_scientific_journal-2520	76	1	(	(	PUNCT
american_scientific_journal-2520	76	2	k-3090	k-3090	PROPN
american_scientific_journal-2520	76	3	)	)	PUNCT
american_scientific_journal-2520	76	4	.	.	PUNCT
american_scientific_journal-2520	77	1	and	and	CCONJ
american_scientific_journal-2520	77	2	the	the	DET
american_scientific_journal-2520	77	3	concentration	concentration	NOUN
american_scientific_journal-2520	77	4	of	of	ADP
american_scientific_journal-2520	77	5	the	the	DET
american_scientific_journal-2520	77	6	extracted	extract	VERB
american_scientific_journal-2520	77	7	rna	rna	NOUN
american_scientific_journal-2520	77	8	was	be	AUX
american_scientific_journal-2520	77	9	determined	determine	VERB
american_scientific_journal-2520	77	10	by	by	ADP
american_scientific_journal-2520	77	11	measuring	measure	VERB
american_scientific_journal-2520	77	12	the	the	DET
american_scientific_journal-2520	77	13	absorbance	absorbance	NOUN
american_scientific_journal-2520	77	14	at	at	ADP
american_scientific_journal-2520	77	15	260	260	NUM
american_scientific_journal-2520	77	16	nm	nm	NOUN
american_scientific_journal-2520	77	17	.	.	PUNCT
american_scientific_journal-2520	78	1	by	by	ADP
american_scientific_journal-2520	78	2	using	use	VERB
american_scientific_journal-2520	78	3	the	the	DET
american_scientific_journal-2520	78	4	spectrophotometer	spectrophotometer	NOUN
american_scientific_journal-2520	78	5	an	an	DET
american_scientific_journal-2520	78	6	absorbance	absorbance	NOUN
american_scientific_journal-2520	78	7	of	of	ADP
american_scientific_journal-2520	78	8	one	one	NUM
american_scientific_journal-2520	78	9	unit	unit	NOUN
american_scientific_journal-2520	78	10	at	at	ADP
american_scientific_journal-2520	78	11	260	260	NUM
american_scientific_journal-2520	78	12	nm	nm	NOUN
american_scientific_journal-2520	78	13	.	.	PUNCT
american_scientific_journal-2520	79	1	agrees	agree	VERB
american_scientific_journal-2520	79	2	to	to	ADP
american_scientific_journal-2520	79	3	40μg	40μg	NOUN
american_scientific_journal-2520	79	4	of	of	ADP
american_scientific_journal-2520	79	5	rna	rna	NOUN
american_scientific_journal-2520	79	6	per	per	ADP
american_scientific_journal-2520	79	7	ml	ml	PROPN
american_scientific_journal-2520	79	8	[	[	X
american_scientific_journal-2520	79	9	13].at	13].at	NUM
american_scientific_journal-2520	79	10	this	this	DET
american_scientific_journal-2520	79	11	way	way	NOUN
american_scientific_journal-2520	79	12	for	for	ADP
american_scientific_journal-2520	79	13	measuring	measure	VERB
american_scientific_journal-2520	79	14	rna	rna	NOUN
american_scientific_journal-2520	79	15	concentration	concentration	NOUN
american_scientific_journal-2520	79	16	about	about	ADP
american_scientific_journal-2520	79	17	10μl	10μl	ADV
american_scientific_journal-2520	79	18	of	of	ADP
american_scientific_journal-2520	79	19	the	the	DET
american_scientific_journal-2520	79	20	extracted	extract	VERB
american_scientific_journal-2520	79	21	bacterial	bacterial	ADJ
american_scientific_journal-2520	79	22	rna	rna	NOUN
american_scientific_journal-2520	79	23	was	be	AUX
american_scientific_journal-2520	79	24	diluted	dilute	VERB
american_scientific_journal-2520	79	25	with	with	ADP
american_scientific_journal-2520	79	26	te	te	ADP
american_scientific_journal-2520	79	27	buffer	buffer	NOUN
american_scientific_journal-2520	79	28	until	until	SCONJ
american_scientific_journal-2520	79	29	the	the	DET
american_scientific_journal-2520	79	30	total	total	ADJ
american_scientific_journal-2520	79	31	volume	volume	NOUN
american_scientific_journal-2520	79	32	reach	reach	VERB
american_scientific_journal-2520	79	33	1000μl	1000μl	ADV
american_scientific_journal-2520	79	34	(	(	PUNCT
american_scientific_journal-2520	79	35	990µl	990µl	NOUN
american_scientific_journal-2520	79	36	of	of	ADP
american_scientific_journal-2520	79	37	the	the	DET
american_scientific_journal-2520	79	38	te	te	PROPN
american_scientific_journal-2520	79	39	buffer	buffer	NOUN
american_scientific_journal-2520	79	40	was	be	AUX
american_scientific_journal-2520	79	41	added	add	VERB
american_scientific_journal-2520	79	42	)	)	PUNCT
american_scientific_journal-2520	79	43	(	(	PUNCT
american_scientific_journal-2520	79	44	1:100	1:100	X
american_scientific_journal-2520	79	45	)	)	PUNCT
american_scientific_journal-2520	79	46	buffer	buffer	NOUN
american_scientific_journal-2520	79	47	.	.	PUNCT
american_scientific_journal-2520	80	1	second	second	ADJ
american_scientific_journal-2520	80	2	step	step	NOUN
american_scientific_journal-2520	80	3	:	:	PUNCT
american_scientific_journal-2520	80	4	optimize	optimize	VERB
american_scientific_journal-2520	80	5	primers	primer	NOUN
american_scientific_journal-2520	80	6	;	;	PUNCT
american_scientific_journal-2520	80	7	the	the	DET
american_scientific_journal-2520	80	8	primers	primer	NOUN
american_scientific_journal-2520	80	9	already	already	ADV
american_scientific_journal-2520	80	10	design	design	VERB
american_scientific_journal-2520	80	11	for	for	ADP
american_scientific_journal-2520	80	12	specific	specific	ADJ
american_scientific_journal-2520	80	13	shpth	shpth	ADJ
american_scientific_journal-2520	80	14	gene	gene	NOUN
american_scientific_journal-2520	80	15	amplification	amplification	NOUN
american_scientific_journal-2520	80	16	.	.	PUNCT
american_scientific_journal-2520	81	1	third	third	ADJ
american_scientific_journal-2520	81	2	step	step	NOUN
american_scientific_journal-2520	81	3	:	:	PUNCT
american_scientific_journal-2520	81	4	reverse	reverse	ADJ
american_scientific_journal-2520	81	5	transcription	transcription	NOUN
american_scientific_journal-2520	81	6	,	,	PUNCT
american_scientific_journal-2520	81	7	the	the	DET
american_scientific_journal-2520	81	8	extracted	extract	VERB
american_scientific_journal-2520	81	9	rna	rna	NOUN
american_scientific_journal-2520	81	10	converted	convert	VERB
american_scientific_journal-2520	81	11	to	to	ADP
american_scientific_journal-2520	81	12	cdna	cdna	PROPN
american_scientific_journal-2520	81	13	by	by	ADP
american_scientific_journal-2520	81	14	used	use	VERB
american_scientific_journal-2520	81	15	goscript	goscript	NOUN
american_scientific_journal-2520	81	16	™	™	ADJ
american_scientific_journal-2520	81	17	reverse	reverse	ADJ
american_scientific_journal-2520	81	18	transcription	transcription	NOUN
american_scientific_journal-2520	81	19	system	system	NOUN
american_scientific_journal-2520	81	20	kit	kit	NOUN
american_scientific_journal-2520	81	21	cat	cat	NOUN
american_scientific_journal-2520	81	22	.	.	PUNCT
american_scientific_journal-2520	82	1	#	#	NOUN
american_scientific_journal-2520	82	2	a5000	a5000	PROPN
american_scientific_journal-2520	82	3	and	and	CCONJ
american_scientific_journal-2520	82	4	random	random	ADJ
american_scientific_journal-2520	82	5	primer	primer	NOUN
american_scientific_journal-2520	82	6	(	(	PUNCT
american_scientific_journal-2520	82	7	0.5μg	0.5μg	NOUN
american_scientific_journal-2520	82	8	/	/	SYM
american_scientific_journal-2520	82	9	reaction	reaction	NOUN
american_scientific_journal-2520	82	10	)	)	PUNCT
american_scientific_journal-2520	82	11	for	for	ADP
american_scientific_journal-2520	82	12	cdna	cdna	PROPN
american_scientific_journal-2520	82	13	synthesis	synthesis	NOUN
american_scientific_journal-2520	82	14	.	.	PUNCT
american_scientific_journal-2520	83	1	fourth	fourth	ADJ
american_scientific_journal-2520	83	2	step	step	NOUN
american_scientific_journal-2520	83	3	:	:	PUNCT
american_scientific_journal-2520	83	4	amplify	amplify	VERB
american_scientific_journal-2520	83	5	cdna	cdna	PROPN
american_scientific_journal-2520	83	6	using	use	VERB
american_scientific_journal-2520	83	7	accupower	accupower	NOUN
american_scientific_journal-2520	83	8	®	®	NOUN
american_scientific_journal-2520	83	9	greenstar	greenstar	NOUN
american_scientific_journal-2520	83	10	™	™	VERB
american_scientific_journal-2520	83	11	qpcr	qpcr	ADJ
american_scientific_journal-2520	83	12	premix	premix	NOUN
american_scientific_journal-2520	83	13	from	from	ADP
american_scientific_journal-2520	83	14	bioneer	bioneer	NOUN
american_scientific_journal-2520	83	15	,	,	PUNCT
american_scientific_journal-2520	83	16	and	and	CCONJ
american_scientific_journal-2520	83	17	the	the	DET
american_scientific_journal-2520	83	18	cdna	cdna	PROPN
american_scientific_journal-2520	83	19	amplification	amplification	NOUN
american_scientific_journal-2520	83	20	was	be	AUX
american_scientific_journal-2520	83	21	carried	carry	VERB
american_scientific_journal-2520	83	22	out	out	ADP
american_scientific_journal-2520	83	23	by	by	ADP
american_scientific_journal-2520	83	24	addition	addition	NOUN
american_scientific_journal-2520	83	25	of	of	ADP
american_scientific_journal-2520	83	26	1μl	1μl	NOUN
american_scientific_journal-2520	83	27	of	of	ADP
american_scientific_journal-2520	83	28	each	each	DET
american_scientific_journal-2520	83	29	f	f	PROPN
american_scientific_journal-2520	83	30	and	and	CCONJ
american_scientific_journal-2520	83	31	r	r	NOUN
american_scientific_journal-2520	83	32	primer	primer	NOUN
american_scientific_journal-2520	83	33	(	(	PUNCT
american_scientific_journal-2520	83	34	10pmol	10pmol	NUM
american_scientific_journal-2520	83	35	)	)	PUNCT
american_scientific_journal-2520	83	36	with	with	ADP
american_scientific_journal-2520	83	37	6μl	6μl	NOUN
american_scientific_journal-2520	83	38	of	of	ADP
american_scientific_journal-2520	83	39	cdna	cdna	PROPN
american_scientific_journal-2520	83	40	and	and	CCONJ
american_scientific_journal-2520	83	41	completes	complete	VERB
american_scientific_journal-2520	83	42	the	the	DET
american_scientific_journal-2520	83	43	volume	volume	NOUN
american_scientific_journal-2520	83	44	to20μl	to20μl	ADV
american_scientific_journal-2520	83	45	depc	depc	NOUN
american_scientific_journal-2520	83	46	-	-	PUNCT
american_scientific_journal-2520	83	47	distilled	distil	VERB
american_scientific_journal-2520	83	48	water	water	NOUN
american_scientific_journal-2520	83	49	.	.	PUNCT
american_scientific_journal-2520	84	1	the	the	DET
american_scientific_journal-2520	84	2	real	real	ADJ
american_scientific_journal-2520	84	3	-	-	PUNCT
american_scientific_journal-2520	84	4	time	time	NOUN
american_scientific_journal-2520	84	5	pcr	pcr	ADJ
american_scientific_journal-2520	84	6	tube	tube	NOUN
american_scientific_journal-2520	84	7	was	be	AUX
american_scientific_journal-2520	84	8	sealed	seal	VERB
american_scientific_journal-2520	84	9	by	by	ADP
american_scientific_journal-2520	84	10	the	the	DET
american_scientific_journal-2520	84	11	optical	optical	ADJ
american_scientific_journal-2520	84	12	adhesive	adhesive	ADJ
american_scientific_journal-2520	84	13	film	film	NOUN
american_scientific_journal-2520	84	14	,	,	PUNCT
american_scientific_journal-2520	84	15	vortex	vortex	NOUN
american_scientific_journal-2520	84	16	and	and	CCONJ
american_scientific_journal-2520	84	17	spin	spin	NOUN
american_scientific_journal-2520	84	18	.	.	PUNCT
american_scientific_journal-2520	85	1	five	five	NUM
american_scientific_journal-2520	85	2	steps	step	NOUN
american_scientific_journal-2520	85	3	:	:	PUNCT
american_scientific_journal-2520	85	4	run	run	NOUN
american_scientific_journal-2520	85	5	reactions	reaction	NOUN
american_scientific_journal-2520	85	6	,	,	PUNCT
american_scientific_journal-2520	85	7	real	real	ADJ
american_scientific_journal-2520	85	8	-	-	PUNCT
american_scientific_journal-2520	85	9	time	time	NOUN
american_scientific_journal-2520	85	10	pcr	pcr	PROPN
american_scientific_journal-2520	85	11	instrument	instrument	NOUN
american_scientific_journal-2520	85	12	was	be	AUX
american_scientific_journal-2520	85	13	start	start	VERB
american_scientific_journal-2520	85	14	and	and	CCONJ
american_scientific_journal-2520	85	15	loaded	load	VERB
american_scientific_journal-2520	85	16	.	.	PUNCT
american_scientific_journal-2520	86	1	the	the	DET
american_scientific_journal-2520	86	2	real	real	ADJ
american_scientific_journal-2520	86	3	time	time	NOUN
american_scientific_journal-2520	86	4	pcr	pcr	PROPN
american_scientific_journal-2520	86	5	setting	setting	NOUN
american_scientific_journal-2520	86	6	was	be	AUX
american_scientific_journal-2520	86	7	programmed.as	programmed.as	X
american_scientific_journal-2520	86	8	5min	5min	NOUN
american_scientific_journal-2520	86	9	.	.	PUNCT
american_scientific_journal-2520	86	10	of	of	ADP
american_scientific_journal-2520	86	11	denaturation	denaturation	NOUN
american_scientific_journal-2520	86	12	at	at	ADP
american_scientific_journal-2520	86	13	95c	95c	NOUN
american_scientific_journal-2520	86	14	°	°	NOUN
american_scientific_journal-2520	86	15	,	,	PUNCT
american_scientific_journal-2520	86	16	45	45	NUM
american_scientific_journal-2520	86	17	cycles	cycle	NOUN
american_scientific_journal-2520	86	18	of	of	ADP
american_scientific_journal-2520	86	19	35sec	35sec	NOUN
american_scientific_journal-2520	86	20	.	.	PUNCT
american_scientific_journal-2520	87	1	at	at	ADP
american_scientific_journal-2520	87	2	95c	95c	NOUN
american_scientific_journal-2520	87	3	°	°	PROPN
american_scientific_journal-2520	87	4	,	,	PUNCT
american_scientific_journal-2520	87	5	1min	1min	NUM
american_scientific_journal-2520	87	6	.	.	PUNCT
american_scientific_journal-2520	88	1	at	at	ADP
american_scientific_journal-2520	88	2	55	55	NUM
american_scientific_journal-2520	88	3	c	c	NOUN
american_scientific_journal-2520	88	4	°	°	NOUN
american_scientific_journal-2520	88	5	,	,	PUNCT
american_scientific_journal-2520	88	6	scan	scan	ADJ
american_scientific_journal-2520	88	7	,	,	PUNCT
american_scientific_journal-2520	88	8	1min	1min	PROPN
american_scientific_journal-2520	88	9	at	at	ADP
american_scientific_journal-2520	88	10	72	72	NUM
american_scientific_journal-2520	88	11	c	c	NOUN
american_scientific_journal-2520	88	12	°	°	NUM
american_scientific_journal-2520	88	13	and	and	CCONJ
american_scientific_journal-2520	88	14	final	final	ADJ
american_scientific_journal-2520	88	15	extension	extension	NOUN
american_scientific_journal-2520	88	16	for	for	ADP
american_scientific_journal-2520	88	17	7min	7min	PROPN
american_scientific_journal-2520	88	18	.	.	PUNCT
american_scientific_journal-2520	89	1	at	at	ADP
american_scientific_journal-2520	89	2	72	72	NUM
american_scientific_journal-2520	89	3	c	c	NOUN
american_scientific_journal-2520	89	4	°	°	NOUN
american_scientific_journal-2520	89	5	melting	melting	NOUN
american_scientific_journal-2520	89	6	and	and	CCONJ
american_scientific_journal-2520	89	7	2	2	NUM
american_scientific_journal-2520	89	8	min	min	NOUN
american_scientific_journal-2520	89	9	.	.	PROPN
american_scientific_journal-2520	89	10	incubation	incubation	NOUN
american_scientific_journal-2520	89	11	at	at	ADP
american_scientific_journal-2520	89	12	25	25	NUM
american_scientific_journal-2520	89	13	c	c	NOUN
american_scientific_journal-2520	89	14	°	°	PROPN
american_scientific_journal-2520	89	15	.	.	PUNCT
american_scientific_journal-2520	90	1	after	after	SCONJ
american_scientific_journal-2520	90	2	reaction	reaction	NOUN
american_scientific_journal-2520	90	3	was	be	AUX
american_scientific_journal-2520	90	4	completed	complete	VERB
american_scientific_journal-2520	90	5	,	,	PUNCT
american_scientific_journal-2520	90	6	data	datum	NOUN
american_scientific_journal-2520	90	7	analysis	analysis	NOUN
american_scientific_journal-2520	90	8	was	be	AUX
american_scientific_journal-2520	90	9	performed	perform	VERB
american_scientific_journal-2520	90	10	.	.	PUNCT
american_scientific_journal-2520	91	1	2.7	2.7	NUM
american_scientific_journal-2520	91	2	.	.	PUNCT
american_scientific_journal-2520	91	3	calculation	calculation	NOUN
american_scientific_journal-2520	91	4	of	of	ADP
american_scientific_journal-2520	91	5	transformation	transformation	NOUN
american_scientific_journal-2520	91	6	efficiency	efficiency	NOUN
american_scientific_journal-2520	91	7	to	to	PART
american_scientific_journal-2520	91	8	measure	measure	VERB
american_scientific_journal-2520	91	9	the	the	DET
american_scientific_journal-2520	91	10	transformation	transformation	NOUN
american_scientific_journal-2520	91	11	efficiency	efficiency	NOUN
american_scientific_journal-2520	91	12	for	for	ADP
american_scientific_journal-2520	91	13	each	each	PRON
american_scientific_journal-2520	91	14	of	of	ADP
american_scientific_journal-2520	91	15	the	the	DET
american_scientific_journal-2520	91	16	competent	competent	ADJ
american_scientific_journal-2520	91	17	strain	strain	NOUN
american_scientific_journal-2520	91	18	,	,	PUNCT
american_scientific_journal-2520	91	19	the	the	DET
american_scientific_journal-2520	91	20	following	follow	VERB
american_scientific_journal-2520	91	21	procedure	procedure	NOUN
american_scientific_journal-2520	91	22	was	be	AUX
american_scientific_journal-2520	91	23	used	use	VERB
american_scientific_journal-2520	91	24	(	(	PUNCT
american_scientific_journal-2520	91	25	invitrogen	invitrogen	PROPN
american_scientific_journal-2520	91	26	):	):	PUNCT
american_scientific_journal-2520	91	27	each	each	PRON
american_scientific_journal-2520	91	28	of	of	ADP
american_scientific_journal-2520	91	29	the	the	DET
american_scientific_journal-2520	91	30	competent	competent	ADJ
american_scientific_journal-2520	91	31	bacterial	bacterial	ADJ
american_scientific_journal-2520	91	32	strain	strain	NOUN
american_scientific_journal-2520	91	33	was	be	AUX
american_scientific_journal-2520	91	34	transformed	transform	VERB
american_scientific_journal-2520	91	35	by	by	ADP
american_scientific_journal-2520	91	36	the	the	DET
american_scientific_journal-2520	91	37	5fold	5fold	NUM
american_scientific_journal-2520	91	38	dilution	dilution	NOUN
american_scientific_journal-2520	91	39	of	of	ADP
american_scientific_journal-2520	91	40	the	the	DET
american_scientific_journal-2520	91	41	ligation	ligation	NOUN
american_scientific_journal-2520	91	42	reaction	reaction	NOUN
american_scientific_journal-2520	91	43	as	as	SCONJ
american_scientific_journal-2520	91	44	described	describe	VERB
american_scientific_journal-2520	91	45	by	by	ADP
american_scientific_journal-2520	91	46	the	the	DET
american_scientific_journal-2520	91	47	protocol	protocol	NOUN
american_scientific_journal-2520	91	48	of	of	ADP
american_scientific_journal-2520	91	49	transformation	transformation	NOUN
american_scientific_journal-2520	91	50	for	for	ADP
american_scientific_journal-2520	91	51	each	each	DET
american_scientific_journal-2520	91	52	bacterial	bacterial	ADJ
american_scientific_journal-2520	91	53	strain	strain	NOUN
american_scientific_journal-2520	91	54	supplied	supply	VERB
american_scientific_journal-2520	91	55	company	company	NOUN
american_scientific_journal-2520	91	56	.	.	PUNCT
american_scientific_journal-2520	92	1	then	then	ADV
american_scientific_journal-2520	92	2	50μl	50μl	ADV
american_scientific_journal-2520	92	3	to	to	PART
american_scientific_journal-2520	92	4	100μl	100μl	ADV
american_scientific_journal-2520	92	5	of	of	ADP
american_scientific_journal-2520	92	6	each	each	DET
american_scientific_journal-2520	92	7	transformed	transform	VERB
american_scientific_journal-2520	92	8	cells	cell	NOUN
american_scientific_journal-2520	92	9	were	be	AUX
american_scientific_journal-2520	92	10	plated	plate	VERB
american_scientific_journal-2520	92	11	on	on	ADP
american_scientific_journal-2520	92	12	lb	lb	DET
american_scientific_journal-2520	92	13	agar	agar	NOUN
american_scientific_journal-2520	92	14	containing	contain	VERB
american_scientific_journal-2520	92	15	ampicillin	ampicillin	PROPN
american_scientific_journal-2520	92	16	,	,	PUNCT
american_scientific_journal-2520	92	17	the	the	DET
american_scientific_journal-2520	92	18	plates	plate	NOUN
american_scientific_journal-2520	92	19	were	be	AUX
american_scientific_journal-2520	92	20	incubated	incubate	VERB
american_scientific_journal-2520	92	21	at	at	ADP
american_scientific_journal-2520	92	22	37	37	NUM
american_scientific_journal-2520	92	23	°	°	NOUN
american_scientific_journal-2520	92	24	c	c	NOUN
american_scientific_journal-2520	92	25	,	,	PUNCT
american_scientific_journal-2520	92	26	overnight	overnight	ADV
american_scientific_journal-2520	92	27	.	.	PUNCT
american_scientific_journal-2520	93	1	the	the	DET
american_scientific_journal-2520	93	2	plates	plate	NOUN
american_scientific_journal-2520	93	3	with	with	ADP
american_scientific_journal-2520	93	4	single	single	ADJ
american_scientific_journal-2520	93	5	colonies	colony	NOUN
american_scientific_journal-2520	93	6	(	(	PUNCT
american_scientific_journal-2520	93	7	10	10	NUM
american_scientific_journal-2520	93	8	100	100	NUM
american_scientific_journal-2520	93	9	)	)	PUNCT
american_scientific_journal-2520	93	10	were	be	AUX
american_scientific_journal-2520	93	11	designated	designate	VERB
american_scientific_journal-2520	93	12	for	for	ADP
american_scientific_journal-2520	93	13	the	the	DET
american_scientific_journal-2520	93	14	calculation	calculation	NOUN
american_scientific_journal-2520	93	15	of	of	ADP
american_scientific_journal-2520	93	16	the	the	DET
american_scientific_journal-2520	93	17	efficiency	efficiency	NOUN
american_scientific_journal-2520	93	18	of	of	ADP
american_scientific_journal-2520	93	19	transformation	transformation	NOUN
american_scientific_journal-2520	93	20	.	.	PUNCT
american_scientific_journal-2520	94	1	the	the	DET
american_scientific_journal-2520	94	2	transformed	transform	VERB
american_scientific_journal-2520	94	3	colonies	colony	NOUN
american_scientific_journal-2520	94	4	were	be	AUX
american_scientific_journal-2520	94	5	counted	count	VERB
american_scientific_journal-2520	94	6	and	and	CCONJ
american_scientific_journal-2520	94	7	the	the	DET
american_scientific_journal-2520	94	8	following	follow	VERB
american_scientific_journal-2520	94	9	equation	equation	NOUN
american_scientific_journal-2520	94	10	was	be	AUX
american_scientific_journal-2520	94	11	used	use	VERB
american_scientific_journal-2520	94	12	:	:	PUNCT
american_scientific_journal-2520	94	13	df	df	NOUN
american_scientific_journal-2520	94	14	:	:	PUNCT
american_scientific_journal-2520	94	15	dilution	dilution	NOUN
american_scientific_journal-2520	94	16	factor	factor	NOUN
american_scientific_journal-2520	94	17	cfu	cfu	PROPN
american_scientific_journal-2520	94	18	:	:	PUNCT
american_scientific_journal-2520	94	19	colony	colony	NOUN
american_scientific_journal-2520	94	20	forming	form	VERB
american_scientific_journal-2520	94	21	unit	unit	NOUN
american_scientific_journal-2520	94	22	3	3	NUM
american_scientific_journal-2520	94	23	.	.	PUNCT
american_scientific_journal-2520	94	24	results	result	VERB
american_scientific_journal-2520	94	25	3.1	3.1	NUM
american_scientific_journal-2520	94	26	construction	construction	NOUN
american_scientific_journal-2520	94	27	of	of	ADP
american_scientific_journal-2520	94	28	synthetic	synthetic	ADJ
american_scientific_journal-2520	94	29	recombinant	recombinant	ADJ
american_scientific_journal-2520	94	30	human	human	ADJ
american_scientific_journal-2520	94	31	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	94	32	hormone	hormone	NOUN
american_scientific_journal-2520	94	33	(	(	PUNCT
american_scientific_journal-2520	94	34	1	1	NUM
american_scientific_journal-2520	94	35	-	-	NUM
american_scientific_journal-2520	94	36	84	84	NUM
american_scientific_journal-2520	94	37	)	)	PUNCT
american_scientific_journal-2520	94	38	gene	gene	NOUN
american_scientific_journal-2520	94	39	(	(	PUNCT
american_scientific_journal-2520	94	40	shpth	shpth	NOUN
american_scientific_journal-2520	94	41	)	)	PUNCT
american_scientific_journal-2520	94	42	a	a	DET
american_scientific_journal-2520	94	43	construction	construction	NOUN
american_scientific_journal-2520	94	44	of	of	ADP
american_scientific_journal-2520	94	45	shpth	shpth	ADJ
american_scientific_journal-2520	94	46	gene	gene	NOUN
american_scientific_journal-2520	94	47	was	be	AUX
american_scientific_journal-2520	94	48	performed	perform	VERB
american_scientific_journal-2520	94	49	by	by	ADP
american_scientific_journal-2520	94	50	splicing	splicing	NOUN
american_scientific_journal-2520	94	51	overlap	overlap	NOUN
american_scientific_journal-2520	94	52	extension	extension	NOUN
american_scientific_journal-2520	94	53	pcr	pcr	NOUN
american_scientific_journal-2520	94	54	and	and	CCONJ
american_scientific_journal-2520	94	55	detected	detect	VERB
american_scientific_journal-2520	94	56	by	by	ADP
american_scientific_journal-2520	94	57	agarose	agarose	NOUN
american_scientific_journal-2520	94	58	gel	gel	NOUN
american_scientific_journal-2520	94	59	https://en.wikipedia.org/wiki/laboratory_technique	https://en.wikipedia.org/wiki/laboratory_technique	NOUN
american_scientific_journal-2520	94	60	https://en.wikipedia.org/wiki/molecular_biology	https://en.wikipedia.org/wiki/molecular_biology	NOUN
american_scientific_journal-2520	94	61	https://en.wikipedia.org/wiki/dna	https://en.wikipedia.org/wiki/dna	PROPN
american_scientific_journal-2520	94	62	https://www.thermofisher.com/iq/en/home/life-science/pcr/real-time-pcr/real-time-pcr-applications/gene-expression-using-real-time-pcr.html	https://www.thermofisher.com/iq/en/home/life-science/pcr/real-time-pcr/real-time-pcr-applications/gene-expression-using-real-time-pcr.html	PROPN
american_scientific_journal-2520	94	63	american	american	PROPN
american_scientific_journal-2520	94	64	scientific	scientific	ADJ
american_scientific_journal-2520	94	65	research	research	NOUN
american_scientific_journal-2520	94	66	journal	journal	NOUN
american_scientific_journal-2520	94	67	for	for	ADP
american_scientific_journal-2520	94	68	engineering	engineering	NOUN
american_scientific_journal-2520	94	69	,	,	PUNCT
american_scientific_journal-2520	94	70	technology	technology	NOUN
american_scientific_journal-2520	94	71	,	,	PUNCT
american_scientific_journal-2520	94	72	and	and	CCONJ
american_scientific_journal-2520	94	73	sciences	science	NOUN
american_scientific_journal-2520	94	74	(	(	PUNCT
american_scientific_journal-2520	94	75	asrjets	asrjet	NOUN
american_scientific_journal-2520	94	76	)	)	PUNCT
american_scientific_journal-2520	94	77	(	(	PUNCT
american_scientific_journal-2520	94	78	2017	2017	NUM
american_scientific_journal-2520	94	79	)	)	PUNCT
american_scientific_journal-2520	94	80	volume	volume	NOUN
american_scientific_journal-2520	94	81	27	27	NUM
american_scientific_journal-2520	94	82	,	,	PUNCT
american_scientific_journal-2520	94	83	no	no	DET
american_scientific_journal-2520	94	84	1	1	NUM
american_scientific_journal-2520	94	85	,	,	PUNCT
american_scientific_journal-2520	94	86	pp	pp	ADV
american_scientific_journal-2520	94	87	12	12	NUM
american_scientific_journal-2520	94	88	-	-	SYM
american_scientific_journal-2520	94	89	24	24	NUM
american_scientific_journal-2520	94	90	17	17	NUM
american_scientific_journal-2520	94	91	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2520	94	92	the	the	DET
american_scientific_journal-2520	94	93	figure	figure	NOUN
american_scientific_journal-2520	94	94	(	(	PUNCT
american_scientific_journal-2520	94	95	1	1	X
american_scientific_journal-2520	94	96	)	)	PUNCT
american_scientific_journal-2520	94	97	show	show	VERB
american_scientific_journal-2520	94	98	the	the	DET
american_scientific_journal-2520	94	99	products	product	NOUN
american_scientific_journal-2520	94	100	of	of	ADP
american_scientific_journal-2520	94	101	the	the	DET
american_scientific_journal-2520	94	102	four	four	NUM
american_scientific_journal-2520	94	103	pcr	pcr	ADJ
american_scientific_journal-2520	94	104	cycles	cycle	NOUN
american_scientific_journal-2520	94	105	carried	carry	VERB
american_scientific_journal-2520	94	106	out	out	ADP
american_scientific_journal-2520	94	107	during	during	ADP
american_scientific_journal-2520	94	108	construction	construction	NOUN
american_scientific_journal-2520	94	109	of	of	ADP
american_scientific_journal-2520	94	110	gene	gene	NOUN
american_scientific_journal-2520	94	111	.	.	PUNCT
american_scientific_journal-2520	95	1	3.2	3.2	NUM
american_scientific_journal-2520	95	2	.	.	PUNCT
american_scientific_journal-2520	96	1	cloning	cloning	NOUN
american_scientific_journal-2520	96	2	of	of	ADP
american_scientific_journal-2520	96	3	synthetic	synthetic	ADJ
american_scientific_journal-2520	96	4	human	human	ADJ
american_scientific_journal-2520	96	5	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	96	6	hormone	hormone	NOUN
american_scientific_journal-2520	96	7	(	(	PUNCT
american_scientific_journal-2520	96	8	shpth	shpth	ADJ
american_scientific_journal-2520	96	9	)	)	PUNCT
american_scientific_journal-2520	96	10	gene	gene	NOUN
american_scientific_journal-2520	96	11	3.2.1	3.2.1	NUM
american_scientific_journal-2520	96	12	.	.	PUNCT
american_scientific_journal-2520	97	1	construction	construction	NOUN
american_scientific_journal-2520	97	2	of	of	ADP
american_scientific_journal-2520	97	3	pet32a	pet32a	NOUN
american_scientific_journal-2520	97	4	-	-	ADJ
american_scientific_journal-2520	97	5	shpth	shpth	ADJ
american_scientific_journal-2520	97	6	vector	vector	NOUN
american_scientific_journal-2520	97	7	the	the	DET
american_scientific_journal-2520	97	8	purified	purify	VERB
american_scientific_journal-2520	97	9	shpth	shpth	ADJ
american_scientific_journal-2520	97	10	gene	gene	NOUN
american_scientific_journal-2520	97	11	as	as	ADV
american_scientific_journal-2520	97	12	well	well	ADV
american_scientific_journal-2520	97	13	as	as	ADP
american_scientific_journal-2520	97	14	the	the	DET
american_scientific_journal-2520	97	15	pet32a	pet32a	NOUN
american_scientific_journal-2520	97	16	(	(	PUNCT
american_scientific_journal-2520	97	17	+	+	NOUN
american_scientific_journal-2520	97	18	)	)	PUNCT
american_scientific_journal-2520	97	19	plasmid	plasmid	NOUN
american_scientific_journal-2520	97	20	were	be	AUX
american_scientific_journal-2520	97	21	restricted	restrict	VERB
american_scientific_journal-2520	97	22	by	by	ADP
american_scientific_journal-2520	97	23	two	two	NUM
american_scientific_journal-2520	97	24	endonuclease	endonuclease	NOUN
american_scientific_journal-2520	97	25	restriction	restriction	NOUN
american_scientific_journal-2520	97	26	enzymes	enzyme	NOUN
american_scientific_journal-2520	97	27	in	in	ADP
american_scientific_journal-2520	97	28	the	the	DET
american_scientific_journal-2520	97	29	sit	sit	NOUN
american_scientific_journal-2520	97	30	(	(	PUNCT
american_scientific_journal-2520	97	31	8	8	NUM
american_scientific_journal-2520	97	32	bp	bp	NOUN
american_scientific_journal-2520	97	33	for	for	ADP
american_scientific_journal-2520	97	34	kpni	kpni	NOUN
american_scientific_journal-2520	97	35	and	and	CCONJ
american_scientific_journal-2520	97	36	281bp	281bp	NOUN
american_scientific_journal-2520	97	37	for	for	ADP
american_scientific_journal-2520	97	38	hindiii	hindiii	NOUN
american_scientific_journal-2520	97	39	of	of	ADP
american_scientific_journal-2520	97	40	the	the	DET
american_scientific_journal-2520	97	41	shpth	shpth	ADJ
american_scientific_journal-2520	97	42	gene	gene	NOUN
american_scientific_journal-2520	97	43	)	)	PUNCT
american_scientific_journal-2520	97	44	and	and	CCONJ
american_scientific_journal-2520	97	45	sit	sit	VERB
american_scientific_journal-2520	97	46	(	(	PUNCT
american_scientific_journal-2520	97	47	238bp	238bp	NOUN
american_scientific_journal-2520	97	48	for	for	ADP
american_scientific_journal-2520	97	49	kpni	kpni	PROPN
american_scientific_journal-2520	97	50	and	and	CCONJ
american_scientific_journal-2520	97	51	173bp	173bp	NOUN
american_scientific_journal-2520	97	52	for	for	ADP
american_scientific_journal-2520	97	53	hindiii	hindiii	NOUN
american_scientific_journal-2520	97	54	of	of	ADP
american_scientific_journal-2520	97	55	pet32a	pet32a	NOUN
american_scientific_journal-2520	97	56	(	(	PUNCT
american_scientific_journal-2520	97	57	+	+	NOUN
american_scientific_journal-2520	97	58	)	)	PUNCT
american_scientific_journal-2520	97	59	)	)	PUNCT
american_scientific_journal-2520	97	60	then	then	ADV
american_scientific_journal-2520	97	61	ligated	ligate	VERB
american_scientific_journal-2520	97	62	to	to	PART
american_scientific_journal-2520	97	63	form	form	VERB
american_scientific_journal-2520	97	64	pet32a	pet32a	NOUN
american_scientific_journal-2520	97	65	-	-	ADJ
american_scientific_journal-2520	97	66	shpth	shpth	ADJ
american_scientific_journal-2520	97	67	vector	vector	NOUN
american_scientific_journal-2520	97	68	as	as	ADP
american_scientific_journal-2520	97	69	in	in	ADP
american_scientific_journal-2520	97	70	figure	figure	NOUN
american_scientific_journal-2520	97	71	(	(	PUNCT
american_scientific_journal-2520	97	72	3	3	NUM
american_scientific_journal-2520	97	73	)	)	PUNCT
american_scientific_journal-2520	97	74	which	which	PRON
american_scientific_journal-2520	97	75	show	show	VERB
american_scientific_journal-2520	97	76	the	the	DET
american_scientific_journal-2520	97	77	pet32ashpth	pet32ashpth	ADJ
american_scientific_journal-2520	97	78	vector	vector	NOUN
american_scientific_journal-2520	97	79	with	with	ADP
american_scientific_journal-2520	97	80	6116	6116	NUM
american_scientific_journal-2520	97	81	bp	bp	PROPN
american_scientific_journal-2520	97	82	in	in	ADP
american_scientific_journal-2520	97	83	accordance	accordance	NOUN
american_scientific_journal-2520	97	84	with	with	ADP
american_scientific_journal-2520	97	85	5900bp	5900bp	NOUN
american_scientific_journal-2520	97	86	of	of	ADP
american_scientific_journal-2520	97	87	pet32a	pet32a	NOUN
american_scientific_journal-2520	97	88	(	(	PUNCT
american_scientific_journal-2520	97	89	+	+	NOUN
american_scientific_journal-2520	97	90	)	)	PUNCT
american_scientific_journal-2520	97	91	plasmid	plasmid	ADJ
american_scientific_journal-2520	97	92	control	control	NOUN
american_scientific_journal-2520	97	93	.	.	PUNCT
american_scientific_journal-2520	98	1	after	after	ADP
american_scientific_journal-2520	98	2	purification	purification	NOUN
american_scientific_journal-2520	98	3	of	of	ADP
american_scientific_journal-2520	98	4	the	the	DET
american_scientific_journal-2520	98	5	restricted	restricted	ADJ
american_scientific_journal-2520	98	6	shpth	shpth	ADJ
american_scientific_journal-2520	98	7	gene	gene	NOUN
american_scientific_journal-2520	98	8	and	and	CCONJ
american_scientific_journal-2520	98	9	pet32a	pet32a	NOUN
american_scientific_journal-2520	98	10	(	(	PUNCT
american_scientific_journal-2520	98	11	+	+	NOUN
american_scientific_journal-2520	98	12	)	)	PUNCT
american_scientific_journal-2520	98	13	,	,	PUNCT
american_scientific_journal-2520	98	14	the	the	DET
american_scientific_journal-2520	98	15	concentration	concentration	NOUN
american_scientific_journal-2520	98	16	of	of	ADP
american_scientific_journal-2520	98	17	restricted	restricted	ADJ
american_scientific_journal-2520	98	18	pet32a	pet32a	NOUN
american_scientific_journal-2520	98	19	(	(	PUNCT
american_scientific_journal-2520	98	20	+	+	NOUN
american_scientific_journal-2520	98	21	)	)	PUNCT
american_scientific_journal-2520	98	22	and	and	CCONJ
american_scientific_journal-2520	98	23	shpth	shpth	ADJ
american_scientific_journal-2520	98	24	gene	gene	NOUN
american_scientific_journal-2520	98	25	was	be	AUX
american_scientific_journal-2520	98	26	(	(	PUNCT
american_scientific_journal-2520	98	27	145and115	145and115	NUM
american_scientific_journal-2520	98	28	)	)	PUNCT
american_scientific_journal-2520	98	29	ng/µl	ng/µl	NUM
american_scientific_journal-2520	98	30	respectively	respectively	ADV
american_scientific_journal-2520	98	31	.	.	PUNCT
american_scientific_journal-2520	99	1	figure	figure	VERB
american_scientific_journal-2520	99	2	1	1	NUM
american_scientific_journal-2520	99	3	:	:	PUNCT
american_scientific_journal-2520	99	4	the	the	DET
american_scientific_journal-2520	99	5	2	2	NUM
american_scientific_journal-2520	99	6	%	%	NOUN
american_scientific_journal-2520	99	7	agarose	agarose	NOUN
american_scientific_journal-2520	99	8	gel	gel	NOUN
american_scientific_journal-2520	99	9	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2520	99	10	for	for	ADP
american_scientific_journal-2520	99	11	construction	construction	NOUN
american_scientific_journal-2520	99	12	of	of	ADP
american_scientific_journal-2520	99	13	shpth	shpth	ADJ
american_scientific_journal-2520	99	14	gene	gene	NOUN
american_scientific_journal-2520	99	15	.	.	PUNCT
american_scientific_journal-2520	100	1	lane	lane	PROPN
american_scientific_journal-2520	100	2	l	l	PROPN
american_scientific_journal-2520	100	3	(	(	PUNCT
american_scientific_journal-2520	100	4	100bp	100bp	ADJ
american_scientific_journal-2520	100	5	dna	dna	NOUN
american_scientific_journal-2520	100	6	ladder	ladder	NOUN
american_scientific_journal-2520	100	7	)	)	PUNCT
american_scientific_journal-2520	100	8	and	and	CCONJ
american_scientific_journal-2520	100	9	lane1	lane1	NOUN
american_scientific_journal-2520	100	10	-	-	PUNCT
american_scientific_journal-2520	100	11	4	4	NUM
american_scientific_journal-2520	100	12	the	the	DET
american_scientific_journal-2520	100	13	products	product	NOUN
american_scientific_journal-2520	100	14	of	of	ADP
american_scientific_journal-2520	100	15	the	the	DET
american_scientific_journal-2520	100	16	four	four	NUM
american_scientific_journal-2520	100	17	pcr	pcr	ADJ
american_scientific_journal-2520	100	18	cycles	cycle	NOUN
american_scientific_journal-2520	100	19	used	use	VERB
american_scientific_journal-2520	100	20	in	in	ADP
american_scientific_journal-2520	100	21	the	the	DET
american_scientific_journal-2520	100	22	construction	construction	NOUN
american_scientific_journal-2520	100	23	of	of	ADP
american_scientific_journal-2520	100	24	the	the	DET
american_scientific_journal-2520	100	25	gene	gene	NOUN
american_scientific_journal-2520	100	26	(	(	PUNCT
american_scientific_journal-2520	100	27	1st	1st	ADJ
american_scientific_journal-2520	100	28	product	product	NOUN
american_scientific_journal-2520	100	29	size	size	NOUN
american_scientific_journal-2520	100	30	(	(	PUNCT
american_scientific_journal-2520	100	31	126bp	126bp	PROPN
american_scientific_journal-2520	100	32	)	)	PUNCT
american_scientific_journal-2520	100	33	,	,	PUNCT
american_scientific_journal-2520	100	34	2nd	2nd	ADJ
american_scientific_journal-2520	100	35	(	(	PUNCT
american_scientific_journal-2520	100	36	181bp	181bp	PROPN
american_scientific_journal-2520	100	37	)	)	PUNCT
american_scientific_journal-2520	100	38	,	,	PUNCT
american_scientific_journal-2520	100	39	3rd	3rd	NOUN
american_scientific_journal-2520	100	40	(	(	PUNCT
american_scientific_journal-2520	100	41	236bp	236bp	ADJ
american_scientific_journal-2520	100	42	)	)	PUNCT
american_scientific_journal-2520	100	43	and	and	CCONJ
american_scientific_journal-2520	100	44	4th	4th	ADJ
american_scientific_journal-2520	100	45	(	(	PUNCT
american_scientific_journal-2520	100	46	289bp	289bp	NOUN
american_scientific_journal-2520	100	47	)	)	PUNCT
american_scientific_journal-2520	100	48	)	)	PUNCT
american_scientific_journal-2520	100	49	.	.	PUNCT
american_scientific_journal-2520	101	1	figure	figure	VERB
american_scientific_journal-2520	101	2	2	2	NUM
american_scientific_journal-2520	101	3	:	:	PUNCT
american_scientific_journal-2520	101	4	the	the	DET
american_scientific_journal-2520	101	5	2	2	NUM
american_scientific_journal-2520	101	6	%	%	NOUN
american_scientific_journal-2520	101	7	agarose	agarose	NOUN
american_scientific_journal-2520	101	8	gel	gel	NOUN
american_scientific_journal-2520	101	9	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2520	101	10	for	for	ADP
american_scientific_journal-2520	101	11	purified	purified	ADJ
american_scientific_journal-2520	101	12	shpth	shpth	ADJ
american_scientific_journal-2520	101	13	gene	gene	NOUN
american_scientific_journal-2520	101	14	lane	lane	NOUN
american_scientific_journal-2520	101	15	g	g	PROPN
american_scientific_journal-2520	101	16	and	and	CCONJ
american_scientific_journal-2520	101	17	lane	lane	PROPN
american_scientific_journal-2520	101	18	m	m	PROPN
american_scientific_journal-2520	101	19	100bp	100bp	ADJ
american_scientific_journal-2520	101	20	dna	dna	PROPN
american_scientific_journal-2520	101	21	ladder	ladder	NOUN
american_scientific_journal-2520	101	22	.	.	PUNCT
american_scientific_journal-2520	102	1	3.2.2	3.2.2	NUM
american_scientific_journal-2520	102	2	transformation	transformation	NOUN
american_scientific_journal-2520	102	3	of	of	ADP
american_scientific_journal-2520	102	4	competent	competent	ADJ
american_scientific_journal-2520	102	5	bacterial	bacterial	ADJ
american_scientific_journal-2520	102	6	cell	cell	NOUN
american_scientific_journal-2520	102	7	the	the	DET
american_scientific_journal-2520	102	8	transformed	transform	VERB
american_scientific_journal-2520	102	9	colonies	colony	NOUN
american_scientific_journal-2520	102	10	were	be	AUX
american_scientific_journal-2520	102	11	grown	grow	VERB
american_scientific_journal-2520	102	12	in	in	ADP
american_scientific_journal-2520	102	13	selective	selective	ADJ
american_scientific_journal-2520	102	14	lb	lb	PRON
american_scientific_journal-2520	102	15	plate	plate	NOUN
american_scientific_journal-2520	102	16	supplied	supply	VERB
american_scientific_journal-2520	102	17	with	with	ADP
american_scientific_journal-2520	102	18	50	50	NUM
american_scientific_journal-2520	102	19	or	or	CCONJ
american_scientific_journal-2520	102	20	100µg	100µg	NOUN
american_scientific_journal-2520	102	21	/	/	SYM
american_scientific_journal-2520	102	22	ml	ml	NOUN
american_scientific_journal-2520	102	23	of	of	ADP
american_scientific_journal-2520	102	24	ampicillin	ampicillin	NOUN
american_scientific_journal-2520	102	25	as	as	SCONJ
american_scientific_journal-2520	102	26	shown	show	VERB
american_scientific_journal-2520	102	27	in	in	ADP
american_scientific_journal-2520	102	28	figure	figure	NOUN
american_scientific_journal-2520	102	29	(	(	PUNCT
american_scientific_journal-2520	102	30	4	4	NUM
american_scientific_journal-2520	102	31	)	)	PUNCT
american_scientific_journal-2520	102	32	.	.	PUNCT
american_scientific_journal-2520	103	1	american	american	PROPN
american_scientific_journal-2520	103	2	scientific	scientific	ADJ
american_scientific_journal-2520	103	3	research	research	PROPN
american_scientific_journal-2520	103	4	journal	journal	NOUN
american_scientific_journal-2520	103	5	for	for	ADP
american_scientific_journal-2520	103	6	engineering	engineering	NOUN
american_scientific_journal-2520	103	7	,	,	PUNCT
american_scientific_journal-2520	103	8	technology	technology	NOUN
american_scientific_journal-2520	103	9	,	,	PUNCT
american_scientific_journal-2520	103	10	and	and	CCONJ
american_scientific_journal-2520	103	11	sciences	science	NOUN
american_scientific_journal-2520	103	12	(	(	PUNCT
american_scientific_journal-2520	103	13	asrjets	asrjet	NOUN
american_scientific_journal-2520	103	14	)	)	PUNCT
american_scientific_journal-2520	103	15	(	(	PUNCT
american_scientific_journal-2520	103	16	2017	2017	NUM
american_scientific_journal-2520	103	17	)	)	PUNCT
american_scientific_journal-2520	103	18	volume	volume	NOUN
american_scientific_journal-2520	103	19	27	27	NUM
american_scientific_journal-2520	103	20	,	,	PUNCT
american_scientific_journal-2520	103	21	no	no	DET
american_scientific_journal-2520	103	22	1	1	NUM
american_scientific_journal-2520	103	23	,	,	PUNCT
american_scientific_journal-2520	103	24	pp	pp	ADV
american_scientific_journal-2520	103	25	12	12	NUM
american_scientific_journal-2520	103	26	-	-	SYM
american_scientific_journal-2520	103	27	24	24	NUM
american_scientific_journal-2520	103	28	18	18	NUM
american_scientific_journal-2520	103	29	figure	figure	NOUN
american_scientific_journal-2520	103	30	3	3	NUM
american_scientific_journal-2520	103	31	:	:	PUNCT
american_scientific_journal-2520	103	32	the	the	DET
american_scientific_journal-2520	103	33	0.8	0.8	NUM
american_scientific_journal-2520	103	34	%	%	NOUN
american_scientific_journal-2520	103	35	agarose	agarose	NOUN
american_scientific_journal-2520	103	36	gel	gel	NOUN
american_scientific_journal-2520	103	37	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2520	103	38	for	for	ADP
american_scientific_journal-2520	103	39	constructed	construct	VERB
american_scientific_journal-2520	103	40	pet32a	pet32a	NOUN
american_scientific_journal-2520	103	41	-	-	ADJ
american_scientific_journal-2520	103	42	shpth	shpth	ADJ
american_scientific_journal-2520	103	43	with	with	ADP
american_scientific_journal-2520	103	44	(	(	PUNCT
american_scientific_journal-2520	103	45	6116bp	6116bp	NOUN
american_scientific_journal-2520	103	46	)	)	PUNCT
american_scientific_journal-2520	103	47	in	in	ADP
american_scientific_journal-2520	103	48	lane	lane	PROPN
american_scientific_journal-2520	103	49	p	p	PROPN
american_scientific_journal-2520	103	50	and	and	CCONJ
american_scientific_journal-2520	103	51	lane	lane	PROPN
american_scientific_journal-2520	103	52	c	c	PROPN
american_scientific_journal-2520	103	53	show	show	VERB
american_scientific_journal-2520	103	54	the	the	DET
american_scientific_journal-2520	103	55	pet32a	pet32a	NOUN
american_scientific_journal-2520	103	56	(	(	PUNCT
american_scientific_journal-2520	103	57	+	+	NOUN
american_scientific_journal-2520	103	58	)	)	PUNCT
american_scientific_journal-2520	103	59	control	control	NOUN
american_scientific_journal-2520	103	60	with	with	ADP
american_scientific_journal-2520	103	61	(	(	PUNCT
american_scientific_journal-2520	103	62	5900bp	5900bp	NOUN
american_scientific_journal-2520	103	63	)	)	PUNCT
american_scientific_journal-2520	103	64	in	in	ADP
american_scientific_journal-2520	103	65	the	the	DET
american_scientific_journal-2520	103	66	right	right	NOUN
american_scientific_journal-2520	103	67	.	.	PUNCT
american_scientific_journal-2520	104	1	and	and	CCONJ
american_scientific_journal-2520	104	2	in	in	ADP
american_scientific_journal-2520	104	3	the	the	DET
american_scientific_journal-2520	104	4	left	left	ADJ
american_scientific_journal-2520	104	5	lane	lane	NOUN
american_scientific_journal-2520	104	6	m	m	AUX
american_scientific_journal-2520	104	7	represent	represent	VERB
american_scientific_journal-2520	104	8	1kbp	1kbp	NUM
american_scientific_journal-2520	104	9	.	.	PUNCT
american_scientific_journal-2520	104	10	ladder	ladder	NOUN
american_scientific_journal-2520	104	11	and	and	CCONJ
american_scientific_journal-2520	104	12	lane	lane	PROPN
american_scientific_journal-2520	104	13	c	c	PROPN
american_scientific_journal-2520	104	14	and	and	CCONJ
american_scientific_journal-2520	104	15	p	p	PROPN
american_scientific_journal-2520	104	16	represent	represent	VERB
american_scientific_journal-2520	104	17	the	the	DET
american_scientific_journal-2520	104	18	pet32a	pet32a	NOUN
american_scientific_journal-2520	104	19	(	(	PUNCT
american_scientific_journal-2520	104	20	+	+	ADJ
american_scientific_journal-2520	104	21	)	)	PUNCT
american_scientific_journal-2520	104	22	control	control	NOUN
american_scientific_journal-2520	104	23	and	and	CCONJ
american_scientific_journal-2520	104	24	constructed	construct	VERB
american_scientific_journal-2520	104	25	pet32a	pet32a	NOUN
american_scientific_journal-2520	104	26	-	-	ADJ
american_scientific_journal-2520	104	27	shpth	shpth	ADJ
american_scientific_journal-2520	104	28	respectively	respectively	ADV
american_scientific_journal-2520	104	29	.	.	PUNCT
american_scientific_journal-2520	105	1	figure	figure	VERB
american_scientific_journal-2520	105	2	4	4	NUM
american_scientific_journal-2520	105	3	:	:	PUNCT
american_scientific_journal-2520	105	4	the	the	DET
american_scientific_journal-2520	105	5	transformed	transform	VERB
american_scientific_journal-2520	105	6	(	(	PUNCT
american_scientific_journal-2520	105	7	a)dh5α	a)dh5α	PROPN
american_scientific_journal-2520	105	8	and	and	CCONJ
american_scientific_journal-2520	105	9	(	(	PUNCT
american_scientific_journal-2520	105	10	b)bl21(de3	b)bl21(de3	NOUN
american_scientific_journal-2520	105	11	)	)	PUNCT
american_scientific_journal-2520	105	12	colonies	colony	NOUN
american_scientific_journal-2520	105	13	.	.	PUNCT
american_scientific_journal-2520	106	1	(	(	PUNCT
american_scientific_journal-2520	106	2	a	a	X
american_scientific_journal-2520	106	3	)	)	PUNCT
american_scientific_journal-2520	106	4	;	;	PUNCT
american_scientific_journal-2520	106	5	(	(	PUNCT
american_scientific_journal-2520	106	6	b	b	X
american_scientific_journal-2520	106	7	)	)	PUNCT
american_scientific_journal-2520	106	8	3.3	3.3	NUM
american_scientific_journal-2520	106	9	.	.	PUNCT
american_scientific_journal-2520	107	1	screening	screen	VERB
american_scientific_journal-2520	107	2	for	for	ADP
american_scientific_journal-2520	107	3	cloning	cloning	NOUN
american_scientific_journal-2520	107	4	isolates	isolate	NOUN
american_scientific_journal-2520	107	5	by	by	ADP
american_scientific_journal-2520	107	6	amplification	amplification	NOUN
american_scientific_journal-2520	107	7	of	of	ADP
american_scientific_journal-2520	107	8	the	the	DET
american_scientific_journal-2520	107	9	target	target	NOUN
american_scientific_journal-2520	107	10	gene	gene	NOUN
american_scientific_journal-2520	107	11	by	by	ADP
american_scientific_journal-2520	107	12	specific	specific	ADJ
american_scientific_journal-2520	107	13	primers	primer	NOUN
american_scientific_journal-2520	107	14	to	to	PART
american_scientific_journal-2520	107	15	screen	screen	VERB
american_scientific_journal-2520	107	16	for	for	ADP
american_scientific_journal-2520	107	17	expression	expression	NOUN
american_scientific_journal-2520	107	18	isolates	isolate	VERB
american_scientific_journal-2520	107	19	the	the	DET
american_scientific_journal-2520	107	20	transforming	transform	VERB
american_scientific_journal-2520	107	21	isolates	isolate	NOUN
american_scientific_journal-2520	107	22	(	(	PUNCT
american_scientific_journal-2520	107	23	both	both	DET
american_scientific_journal-2520	107	24	e.	e.	PROPN
american_scientific_journal-2520	107	25	coli	coli	NOUN
american_scientific_journal-2520	108	1	bl21	bl21	PROPN
american_scientific_journal-2520	108	2	(	(	PUNCT
american_scientific_journal-2520	108	3	de3	de3	PROPN
american_scientific_journal-2520	108	4	)	)	PUNCT
american_scientific_journal-2520	108	5	and	and	CCONJ
american_scientific_journal-2520	108	6	dh5α	dh5α	NUM
american_scientific_journal-2520	108	7	)	)	PUNCT
american_scientific_journal-2520	108	8	were	be	AUX
american_scientific_journal-2520	108	9	detected	detect	VERB
american_scientific_journal-2520	108	10	to	to	PART
american_scientific_journal-2520	108	11	have	have	VERB
american_scientific_journal-2520	108	12	the	the	DET
american_scientific_journal-2520	108	13	desired	desire	VERB
american_scientific_journal-2520	108	14	recombinant	recombinant	ADJ
american_scientific_journal-2520	108	15	pet32a	pet32a	NOUN
american_scientific_journal-2520	108	16	-	-	ADJ
american_scientific_journal-2520	108	17	shpth	shpth	ADJ
american_scientific_journal-2520	108	18	vector	vector	NOUN
american_scientific_journal-2520	108	19	by	by	ADP
american_scientific_journal-2520	108	20	amplifying	amplify	VERB
american_scientific_journal-2520	108	21	the	the	DET
american_scientific_journal-2520	108	22	target	target	NOUN
american_scientific_journal-2520	108	23	shpth	shpth	ADJ
american_scientific_journal-2520	108	24	gene	gene	NOUN
american_scientific_journal-2520	108	25	which	which	PRON
american_scientific_journal-2520	108	26	inserted	insert	VERB
american_scientific_journal-2520	108	27	in	in	ADP
american_scientific_journal-2520	108	28	that	that	DET
american_scientific_journal-2520	108	29	vector	vector	NOUN
american_scientific_journal-2520	108	30	,	,	PUNCT
american_scientific_journal-2520	108	31	the	the	DET
american_scientific_journal-2520	108	32	later	later	ADV
american_scientific_journal-2520	108	33	was	be	AUX
american_scientific_journal-2520	108	34	extracted	extract	VERB
american_scientific_journal-2520	108	35	from	from	ADP
american_scientific_journal-2520	108	36	transformed	transform	VERB
american_scientific_journal-2520	108	37	colonies	colony	NOUN
american_scientific_journal-2520	108	38	and	and	CCONJ
american_scientific_journal-2520	108	39	used	use	VERB
american_scientific_journal-2520	108	40	as	as	ADP
american_scientific_journal-2520	108	41	a	a	DET
american_scientific_journal-2520	108	42	template	template	NOUN
american_scientific_journal-2520	108	43	for	for	ADP
american_scientific_journal-2520	108	44	shpth	shpth	ADJ
american_scientific_journal-2520	108	45	gene	gene	NOUN
american_scientific_journal-2520	108	46	amplification	amplification	NOUN
american_scientific_journal-2520	108	47	as	as	SCONJ
american_scientific_journal-2520	108	48	shown	show	VERB
american_scientific_journal-2520	108	49	in	in	ADP
american_scientific_journal-2520	108	50	the	the	DET
american_scientific_journal-2520	108	51	figure	figure	NOUN
american_scientific_journal-2520	108	52	(	(	PUNCT
american_scientific_journal-2520	108	53	5	5	NUM
american_scientific_journal-2520	108	54	)	)	PUNCT
american_scientific_journal-2520	108	55	,	,	PUNCT
american_scientific_journal-2520	108	56	and	and	CCONJ
american_scientific_journal-2520	108	57	(	(	PUNCT
american_scientific_journal-2520	108	58	6	6	NUM
american_scientific_journal-2520	108	59	)	)	PUNCT
american_scientific_journal-2520	108	60	which	which	PRON
american_scientific_journal-2520	108	61	show	show	VERB
american_scientific_journal-2520	108	62	the	the	DET
american_scientific_journal-2520	108	63	agarose	agarose	NOUN
american_scientific_journal-2520	108	64	gel	gel	NOUN
american_scientific_journal-2520	108	65	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2520	108	66	of	of	ADP
american_scientific_journal-2520	108	67	amplifying	amplify	VERB
american_scientific_journal-2520	108	68	shpth	shpth	ADJ
american_scientific_journal-2520	108	69	gene	gene	NOUN
american_scientific_journal-2520	108	70	.	.	PUNCT
american_scientific_journal-2520	109	1	3.4	3.4	NUM
american_scientific_journal-2520	109	2	.	.	PUNCT
american_scientific_journal-2520	109	3	detection	detection	NOUN
american_scientific_journal-2520	109	4	of	of	ADP
american_scientific_journal-2520	109	5	gene	gene	NOUN
american_scientific_journal-2520	109	6	expression	expression	NOUN
american_scientific_journal-2520	109	7	by	by	ADP
american_scientific_journal-2520	109	8	real	real	ADJ
american_scientific_journal-2520	109	9	time	time	NOUN
american_scientific_journal-2520	109	10	pcr	pcr	PROPN
american_scientific_journal-2520	109	11	the	the	DET
american_scientific_journal-2520	109	12	rna	rna	NOUN
american_scientific_journal-2520	109	13	of	of	ADP
american_scientific_journal-2520	109	14	transformed	transform	VERB
american_scientific_journal-2520	109	15	isolates	isolate	NOUN
american_scientific_journal-2520	109	16	was	be	AUX
american_scientific_journal-2520	109	17	extracted	extract	VERB
american_scientific_journal-2520	109	18	after	after	ADP
american_scientific_journal-2520	109	19	induction	induction	NOUN
american_scientific_journal-2520	109	20	period	period	NOUN
american_scientific_journal-2520	109	21	of	of	ADP
american_scientific_journal-2520	109	22	the	the	DET
american_scientific_journal-2520	109	23	transformed	transform	VERB
american_scientific_journal-2520	109	24	isolates	isolate	NOUN
american_scientific_journal-2520	109	25	by	by	ADP
american_scientific_journal-2520	109	26	iptg	iptg	PROPN
american_scientific_journal-2520	109	27	.	.	PUNCT
american_scientific_journal-2520	110	1	and	and	CCONJ
american_scientific_journal-2520	110	2	the	the	DET
american_scientific_journal-2520	110	3	rna	rna	NOUN
american_scientific_journal-2520	110	4	concentration	concentration	NOUN
american_scientific_journal-2520	110	5	was	be	AUX
american_scientific_journal-2520	110	6	10	10	NUM
american_scientific_journal-2520	110	7	µg	µg	NOUN
american_scientific_journal-2520	110	8	/	/	SYM
american_scientific_journal-2520	110	9	ml	ml	NOUN
american_scientific_journal-2520	110	10	.	.	PUNCT
american_scientific_journal-2520	111	1	the	the	DET
american_scientific_journal-2520	111	2	extracted	extract	VERB
american_scientific_journal-2520	111	3	rna	rna	NOUN
american_scientific_journal-2520	111	4	was	be	AUX
american_scientific_journal-2520	111	5	converted	convert	VERB
american_scientific_journal-2520	111	6	to	to	ADP
american_scientific_journal-2520	111	7	cdna	cdna	PROPN
american_scientific_journal-2520	111	8	by	by	ADP
american_scientific_journal-2520	111	9	using	use	VERB
american_scientific_journal-2520	111	10	goscript	goscript	NOUN
american_scientific_journal-2520	111	11	™	™	ADJ
american_scientific_journal-2520	111	12	reverse	reverse	ADJ
american_scientific_journal-2520	111	13	transcription	transcription	NOUN
american_scientific_journal-2520	111	14	system	system	NOUN
american_scientific_journal-2520	111	15	and	and	CCONJ
american_scientific_journal-2520	111	16	random	random	ADJ
american_scientific_journal-2520	111	17	primer	primer	NOUN
american_scientific_journal-2520	111	18	in	in	ADP
american_scientific_journal-2520	111	19	the	the	DET
american_scientific_journal-2520	111	20	reaction	reaction	NOUN
american_scientific_journal-2520	111	21	tube	tube	NOUN
american_scientific_journal-2520	111	22	.	.	PUNCT
american_scientific_journal-2520	112	1	and	and	CCONJ
american_scientific_journal-2520	112	2	then	then	ADV
american_scientific_journal-2520	112	3	real	real	ADJ
american_scientific_journal-2520	112	4	time	time	NOUN
american_scientific_journal-2520	112	5	rt	rt	PROPN
american_scientific_journal-2520	112	6	pcr	pcr	PROPN
american_scientific_journal-2520	112	7	was	be	AUX
american_scientific_journal-2520	112	8	carried	carry	VERB
american_scientific_journal-2520	112	9	out	out	ADP
american_scientific_journal-2520	112	10	which	which	PRON
american_scientific_journal-2520	112	11	show	show	VERB
american_scientific_journal-2520	112	12	a	a	DET
american_scientific_journal-2520	112	13	high	high	ADJ
american_scientific_journal-2520	112	14	shpth	shpth	ADJ
american_scientific_journal-2520	112	15	gene	gene	NOUN
american_scientific_journal-2520	112	16	expression	expression	NOUN
american_scientific_journal-2520	112	17	as	as	SCONJ
american_scientific_journal-2520	112	18	shown	show	VERB
american_scientific_journal-2520	112	19	in	in	ADP
american_scientific_journal-2520	112	20	figure	figure	NOUN
american_scientific_journal-2520	112	21	(	(	PUNCT
american_scientific_journal-2520	112	22	7	7	NUM
american_scientific_journal-2520	112	23	)	)	PUNCT
american_scientific_journal-2520	112	24	of	of	ADP
american_scientific_journal-2520	112	25	the	the	DET
american_scientific_journal-2520	112	26	fluorescent	fluorescent	NOUN
american_scientific_journal-2520	112	27	(	(	PUNCT
american_scientific_journal-2520	112	28	threshold	threshold	NOUN
american_scientific_journal-2520	112	29	cycle	cycle	NOUN
american_scientific_journal-2520	112	30	)	)	PUNCT
american_scientific_journal-2520	112	31	curve	curve	NOUN
american_scientific_journal-2520	112	32	with	with	ADP
american_scientific_journal-2520	112	33	ct	ct	NUM
american_scientific_journal-2520	112	34	value	value	NOUN
american_scientific_journal-2520	112	35	of	of	ADP
american_scientific_journal-2520	112	36	about	about	ADP
american_scientific_journal-2520	112	37	(	(	PUNCT
american_scientific_journal-2520	112	38	23.46	23.46	NUM
american_scientific_journal-2520	112	39	)	)	PUNCT
american_scientific_journal-2520	112	40	and	and	CCONJ
american_scientific_journal-2520	112	41	figure	figure	NOUN
american_scientific_journal-2520	112	42	(	(	PUNCT
american_scientific_journal-2520	112	43	8)	8)	NUM
american_scientific_journal-2520	112	44	for	for	ADP
american_scientific_journal-2520	112	45	melting	melt	VERB
american_scientific_journal-2520	112	46	curve	curve	NOUN
american_scientific_journal-2520	112	47	and	and	CCONJ
american_scientific_journal-2520	112	48	the	the	DET
american_scientific_journal-2520	112	49	gene	gene	NOUN
american_scientific_journal-2520	112	50	.	.	PUNCT
american_scientific_journal-2520	113	1	american	american	ADJ
american_scientific_journal-2520	113	2	scientific	scientific	ADJ
american_scientific_journal-2520	113	3	research	research	PROPN
american_scientific_journal-2520	113	4	journal	journal	NOUN
american_scientific_journal-2520	113	5	for	for	ADP
american_scientific_journal-2520	113	6	engineering	engineering	NOUN
american_scientific_journal-2520	113	7	,	,	PUNCT
american_scientific_journal-2520	113	8	technology	technology	NOUN
american_scientific_journal-2520	113	9	,	,	PUNCT
american_scientific_journal-2520	113	10	and	and	CCONJ
american_scientific_journal-2520	113	11	sciences	science	NOUN
american_scientific_journal-2520	113	12	(	(	PUNCT
american_scientific_journal-2520	113	13	asrjets	asrjet	NOUN
american_scientific_journal-2520	113	14	)	)	PUNCT
american_scientific_journal-2520	113	15	(	(	PUNCT
american_scientific_journal-2520	113	16	2017	2017	NUM
american_scientific_journal-2520	113	17	)	)	PUNCT
american_scientific_journal-2520	113	18	volume	volume	NOUN
american_scientific_journal-2520	113	19	27	27	NUM
american_scientific_journal-2520	113	20	,	,	PUNCT
american_scientific_journal-2520	113	21	no	no	DET
american_scientific_journal-2520	113	22	1	1	NUM
american_scientific_journal-2520	113	23	,	,	PUNCT
american_scientific_journal-2520	113	24	pp	pp	ADV
american_scientific_journal-2520	113	25	12	12	NUM
american_scientific_journal-2520	113	26	-	-	SYM
american_scientific_journal-2520	113	27	24	24	NUM
american_scientific_journal-2520	113	28	19	19	NUM
american_scientific_journal-2520	113	29	figure	figure	NOUN
american_scientific_journal-2520	113	30	5	5	NUM
american_scientific_journal-2520	113	31	:	:	PUNCT
american_scientific_journal-2520	113	32	the	the	DET
american_scientific_journal-2520	113	33	agarose	agarose	NOUN
american_scientific_journal-2520	113	34	gel	gel	NOUN
american_scientific_journal-2520	113	35	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2520	113	36	for	for	SCONJ
american_scientific_journal-2520	113	37	amplified	amplify	VERB
american_scientific_journal-2520	113	38	shpth	shpth	ADJ
american_scientific_journal-2520	113	39	gene	gene	NOUN
american_scientific_journal-2520	113	40	.	.	PUNCT
american_scientific_journal-2520	114	1	this	this	PRON
american_scientific_journal-2520	114	2	amplified	amplify	VERB
american_scientific_journal-2520	114	3	from	from	ADP
american_scientific_journal-2520	114	4	recombinant	recombinant	ADJ
american_scientific_journal-2520	114	5	pet32a	pet32a	NOUN
american_scientific_journal-2520	114	6	-	-	ADJ
american_scientific_journal-2520	114	7	shpth	shpth	ADJ
american_scientific_journal-2520	114	8	vector	vector	NOUN
american_scientific_journal-2520	114	9	extracted	extract	VERB
american_scientific_journal-2520	114	10	from	from	ADP
american_scientific_journal-2520	114	11	transformed	transform	VERB
american_scientific_journal-2520	114	12	bl21	bl21	PROPN
american_scientific_journal-2520	114	13	(	(	PUNCT
american_scientific_journal-2520	114	14	de3	de3	PROPN
american_scientific_journal-2520	114	15	)	)	PUNCT
american_scientific_journal-2520	114	16	.	.	PUNCT
american_scientific_journal-2520	115	1	the	the	DET
american_scientific_journal-2520	115	2	lane	lane	PROPN
american_scientific_journal-2520	115	3	m	m	AUX
american_scientific_journal-2520	115	4	represent	represent	VERB
american_scientific_journal-2520	115	5	100bp	100bp	ADJ
american_scientific_journal-2520	115	6	dna	dna	PROPN
american_scientific_journal-2520	115	7	ladder	ladder	NOUN
american_scientific_journal-2520	115	8	and	and	CCONJ
american_scientific_journal-2520	115	9	lane1	lane1	PROPN
american_scientific_journal-2520	115	10	,	,	PUNCT
american_scientific_journal-2520	115	11	2	2	NUM
american_scientific_journal-2520	115	12	represent	represent	VERB
american_scientific_journal-2520	115	13	the	the	DET
american_scientific_journal-2520	115	14	amplified	amplified	ADJ
american_scientific_journal-2520	115	15	shpth	shpth	ADJ
american_scientific_journal-2520	115	16	gene	gene	NOUN
american_scientific_journal-2520	115	17	.	.	PUNCT
american_scientific_journal-2520	116	1	figure	figure	VERB
american_scientific_journal-2520	116	2	6	6	NUM
american_scientific_journal-2520	116	3	:	:	PUNCT
american_scientific_journal-2520	116	4	the	the	DET
american_scientific_journal-2520	116	5	agarose	agarose	NOUN
american_scientific_journal-2520	116	6	gel	gel	NOUN
american_scientific_journal-2520	116	7	electrophoresis	electrophoresis	NOUN
american_scientific_journal-2520	116	8	for	for	SCONJ
american_scientific_journal-2520	116	9	amplified	amplify	VERB
american_scientific_journal-2520	116	10	shpth	shpth	ADJ
american_scientific_journal-2520	116	11	gene	gene	NOUN
american_scientific_journal-2520	116	12	.	.	PUNCT
american_scientific_journal-2520	117	1	this	this	PRON
american_scientific_journal-2520	117	2	amplified	amplify	VERB
american_scientific_journal-2520	117	3	from	from	ADP
american_scientific_journal-2520	117	4	recombinant	recombinant	ADJ
american_scientific_journal-2520	117	5	pet32a	pet32a	NOUN
american_scientific_journal-2520	117	6	-	-	ADJ
american_scientific_journal-2520	117	7	shpth	shpth	ADJ
american_scientific_journal-2520	117	8	vector	vector	NOUN
american_scientific_journal-2520	117	9	extracted	extract	VERB
american_scientific_journal-2520	117	10	from	from	ADP
american_scientific_journal-2520	117	11	transformed	transform	VERB
american_scientific_journal-2520	117	12	dh5α	dh5α	PROPN
american_scientific_journal-2520	117	13	.	.	PUNCT
american_scientific_journal-2520	118	1	the	the	DET
american_scientific_journal-2520	118	2	lane	lane	NOUN
american_scientific_journal-2520	118	3	m	m	AUX
american_scientific_journal-2520	118	4	represent	represent	VERB
american_scientific_journal-2520	118	5	100bp	100bp	ADJ
american_scientific_journal-2520	118	6	dna	dna	PROPN
american_scientific_journal-2520	118	7	ladder	ladder	NOUN
american_scientific_journal-2520	118	8	and	and	CCONJ
american_scientific_journal-2520	118	9	lane1	lane1	PROPN
american_scientific_journal-2520	118	10	represent	represent	VERB
american_scientific_journal-2520	118	11	the	the	DET
american_scientific_journal-2520	118	12	amplified	amplified	ADJ
american_scientific_journal-2520	118	13	shpth	shpth	ADJ
american_scientific_journal-2520	118	14	gene	gene	NOUN
american_scientific_journal-2520	118	15	.	.	PUNCT
american_scientific_journal-2520	119	1	figure	figure	VERB
american_scientific_journal-2520	119	2	7	7	NUM
american_scientific_journal-2520	119	3	:	:	PUNCT
american_scientific_journal-2520	119	4	the	the	DET
american_scientific_journal-2520	119	5	fluorescent	fluorescent	NOUN
american_scientific_journal-2520	119	6	ct	ct	NUM
american_scientific_journal-2520	119	7	curve	curve	NOUN
american_scientific_journal-2520	119	8	for	for	ADP
american_scientific_journal-2520	119	9	shpth	shpth	ADJ
american_scientific_journal-2520	119	10	gene	gene	NOUN
american_scientific_journal-2520	119	11	.	.	PUNCT
american_scientific_journal-2520	120	1	american	american	ADJ
american_scientific_journal-2520	120	2	scientific	scientific	ADJ
american_scientific_journal-2520	120	3	research	research	PROPN
american_scientific_journal-2520	120	4	journal	journal	NOUN
american_scientific_journal-2520	120	5	for	for	ADP
american_scientific_journal-2520	120	6	engineering	engineering	NOUN
american_scientific_journal-2520	120	7	,	,	PUNCT
american_scientific_journal-2520	120	8	technology	technology	NOUN
american_scientific_journal-2520	120	9	,	,	PUNCT
american_scientific_journal-2520	120	10	and	and	CCONJ
american_scientific_journal-2520	120	11	sciences	science	NOUN
american_scientific_journal-2520	120	12	(	(	PUNCT
american_scientific_journal-2520	120	13	asrjets	asrjet	NOUN
american_scientific_journal-2520	120	14	)	)	PUNCT
american_scientific_journal-2520	120	15	(	(	PUNCT
american_scientific_journal-2520	120	16	2017	2017	NUM
american_scientific_journal-2520	120	17	)	)	PUNCT
american_scientific_journal-2520	120	18	volume	volume	NOUN
american_scientific_journal-2520	120	19	27	27	NUM
american_scientific_journal-2520	120	20	,	,	PUNCT
american_scientific_journal-2520	120	21	no	no	DET
american_scientific_journal-2520	120	22	1	1	NUM
american_scientific_journal-2520	120	23	,	,	PUNCT
american_scientific_journal-2520	120	24	pp	pp	ADV
american_scientific_journal-2520	120	25	12	12	NUM
american_scientific_journal-2520	120	26	-	-	SYM
american_scientific_journal-2520	120	27	24	24	NUM
american_scientific_journal-2520	120	28	20	20	NUM
american_scientific_journal-2520	120	29	figure	figure	NOUN
american_scientific_journal-2520	120	30	8	8	NUM
american_scientific_journal-2520	120	31	:	:	PUNCT
american_scientific_journal-2520	120	32	the	the	DET
american_scientific_journal-2520	120	33	melting	melt	VERB
american_scientific_journal-2520	120	34	curve	curve	NOUN
american_scientific_journal-2520	120	35	of	of	ADP
american_scientific_journal-2520	120	36	real	real	ADJ
american_scientific_journal-2520	120	37	time	time	NOUN
american_scientific_journal-2520	120	38	rt	rt	PROPN
american_scientific_journal-2520	120	39	pcr	pcr	PROPN
american_scientific_journal-2520	120	40	for	for	ADP
american_scientific_journal-2520	120	41	shpth	shpth	ADJ
american_scientific_journal-2520	120	42	gene	gene	NOUN
american_scientific_journal-2520	120	43	.	.	PUNCT
american_scientific_journal-2520	121	1	3.5	3.5	NUM
american_scientific_journal-2520	121	2	.	.	PUNCT
american_scientific_journal-2520	122	1	transformation	transformation	NOUN
american_scientific_journal-2520	122	2	efficiency	efficiency	NOUN
american_scientific_journal-2520	122	3	the	the	DET
american_scientific_journal-2520	122	4	transformation	transformation	NOUN
american_scientific_journal-2520	122	5	efficiency	efficiency	NOUN
american_scientific_journal-2520	122	6	for	for	ADP
american_scientific_journal-2520	122	7	e.	e.	PROPN
american_scientific_journal-2520	122	8	coli	coli	NOUN
american_scientific_journal-2520	123	1	bl21	bl21	PROPN
american_scientific_journal-2520	123	2	(	(	PUNCT
american_scientific_journal-2520	123	3	de3	de3	PROPN
american_scientific_journal-2520	123	4	)	)	PUNCT
american_scientific_journal-2520	123	5	and	and	CCONJ
american_scientific_journal-2520	123	6	dh5α	dh5α	PROPN
american_scientific_journal-2520	123	7	was	be	AUX
american_scientific_journal-2520	123	8	6.6	6.6	NUM
american_scientific_journal-2520	123	9	*	*	SYM
american_scientific_journal-2520	123	10	106	106	NUM
american_scientific_journal-2520	123	11	and	and	CCONJ
american_scientific_journal-2520	123	12	3.4	3.4	NUM
american_scientific_journal-2520	123	13	*	*	SYM
american_scientific_journal-2520	123	14	106	106	NUM
american_scientific_journal-2520	123	15	respectively	respectively	ADV
american_scientific_journal-2520	123	16	.	.	PUNCT
american_scientific_journal-2520	124	1	4	4	X
american_scientific_journal-2520	124	2	.	.	X
american_scientific_journal-2520	124	3	discussion	discussion	NOUN
american_scientific_journal-2520	124	4	the	the	DET
american_scientific_journal-2520	124	5	hpth	hpth	NOUN
american_scientific_journal-2520	124	6	gene	gene	NOUN
american_scientific_journal-2520	124	7	mrna	mrna	PROPN
american_scientific_journal-2520	124	8	is	be	AUX
american_scientific_journal-2520	124	9	translated	translate	VERB
american_scientific_journal-2520	124	10	to	to	PART
american_scientific_journal-2520	124	11	give	give	VERB
american_scientific_journal-2520	124	12	a	a	DET
american_scientific_journal-2520	124	13	prepro	prepro	ADJ
american_scientific_journal-2520	124	14	-	-	PUNCT
american_scientific_journal-2520	124	15	hormone	hormone	NOUN
american_scientific_journal-2520	124	16	with	with	ADP
american_scientific_journal-2520	124	17	115	115	NUM
american_scientific_journal-2520	124	18	amino	amino	NOUN
american_scientific_journal-2520	124	19	acid	acid	NOUN
american_scientific_journal-2520	124	20	(	(	PUNCT
american_scientific_journal-2520	124	21	biologically	biologically	ADV
american_scientific_journal-2520	124	22	in	in	ADP
american_scientific_journal-2520	124	23	active	active	ADJ
american_scientific_journal-2520	124	24	hormone	hormone	NOUN
american_scientific_journal-2520	124	25	)	)	PUNCT
american_scientific_journal-2520	124	26	therefore	therefore	ADV
american_scientific_journal-2520	124	27	cloning	cloning	NOUN
american_scientific_journal-2520	124	28	of	of	ADP
american_scientific_journal-2520	124	29	cdna	cdna	PROPN
american_scientific_journal-2520	124	30	of	of	ADP
american_scientific_journal-2520	124	31	the	the	DET
american_scientific_journal-2520	124	32	hpth	hpth	NOUN
american_scientific_journal-2520	124	33	gene	gene	NOUN
american_scientific_journal-2520	124	34	was	be	AUX
american_scientific_journal-2520	124	35	not	not	PART
american_scientific_journal-2520	124	36	attractive	attractive	ADJ
american_scientific_journal-2520	124	37	for	for	ADP
american_scientific_journal-2520	124	38	the	the	DET
american_scientific_journal-2520	124	39	researcher	researcher	NOUN
american_scientific_journal-2520	124	40	who	who	PRON
american_scientific_journal-2520	124	41	seek	seek	VERB
american_scientific_journal-2520	124	42	for	for	ADP
american_scientific_journal-2520	124	43	hormone	hormone	NOUN
american_scientific_journal-2520	124	44	activity	activity	NOUN
american_scientific_journal-2520	124	45	.	.	PUNCT
american_scientific_journal-2520	125	1	since	since	SCONJ
american_scientific_journal-2520	125	2	1983	1983	NUM
american_scientific_journal-2520	125	3	,	,	PUNCT
american_scientific_journal-2520	125	4	vasicek	vasicek	PROPN
american_scientific_journal-2520	125	5	and	and	CCONJ
american_scientific_journal-2520	125	6	his	his	PRON
american_scientific_journal-2520	125	7	colleagues	colleague	NOUN
american_scientific_journal-2520	125	8	found	find	VERB
american_scientific_journal-2520	125	9	the	the	DET
american_scientific_journal-2520	125	10	hpth	hpth	NOUN
american_scientific_journal-2520	125	11	gene	gene	NOUN
american_scientific_journal-2520	125	12	in	in	ADP
american_scientific_journal-2520	125	13	the	the	DET
american_scientific_journal-2520	125	14	a	a	PRON
american_scientific_journal-2520	125	15	λ	λ	PROPN
american_scientific_journal-2520	125	16	phage	phage	NOUN
american_scientific_journal-2520	125	17	gene	gene	NOUN
american_scientific_journal-2520	125	18	library	library	NOUN
american_scientific_journal-2520	125	19	and	and	CCONJ
american_scientific_journal-2520	125	20	determined	determine	VERB
american_scientific_journal-2520	125	21	the	the	DET
american_scientific_journal-2520	125	22	sequence	sequence	NOUN
american_scientific_journal-2520	125	23	of	of	ADP
american_scientific_journal-2520	125	24	each	each	DET
american_scientific_journal-2520	125	25	parts	part	NOUN
american_scientific_journal-2520	125	26	of	of	ADP
american_scientific_journal-2520	125	27	the	the	DET
american_scientific_journal-2520	125	28	gene	gene	NOUN
american_scientific_journal-2520	125	29	,	,	PUNCT
american_scientific_journal-2520	125	30	the	the	DET
american_scientific_journal-2520	125	31	active	active	ADJ
american_scientific_journal-2520	125	32	gene	gene	NOUN
american_scientific_journal-2520	125	33	sites	site	NOUN
american_scientific_journal-2520	125	34	was	be	AUX
american_scientific_journal-2520	125	35	restricted	restrict	VERB
american_scientific_journal-2520	125	36	from	from	ADP
american_scientific_journal-2520	125	37	the	the	DET
american_scientific_journal-2520	125	38	total	total	ADJ
american_scientific_journal-2520	125	39	gene	gene	NOUN
american_scientific_journal-2520	125	40	or	or	CCONJ
american_scientific_journal-2520	125	41	gene	gene	NOUN
american_scientific_journal-2520	125	42	cdna	cdna	PROPN
american_scientific_journal-2520	125	43	which	which	PRON
american_scientific_journal-2520	125	44	inserted	insert	VERB
american_scientific_journal-2520	125	45	in	in	ADP
american_scientific_journal-2520	125	46	a	a	DET
american_scientific_journal-2520	125	47	multiple	multiple	ADJ
american_scientific_journal-2520	125	48	plasmid	plasmid	NOUN
american_scientific_journal-2520	125	49	like	like	ADP
american_scientific_journal-2520	125	50	psshpth102	psshpth102	PROPN
american_scientific_journal-2520	125	51	vector	vector	NOUN
american_scientific_journal-2520	125	52	[	[	X
american_scientific_journal-2520	125	53	14	14	NUM
american_scientific_journal-2520	125	54	]	]	PUNCT
american_scientific_journal-2520	125	55	.	.	PUNCT
american_scientific_journal-2520	126	1	all	all	PRON
american_scientific_journal-2520	126	2	that	that	PRON
american_scientific_journal-2520	126	3	led	lead	VERB
american_scientific_journal-2520	126	4	the	the	DET
american_scientific_journal-2520	126	5	researcher	researcher	NOUN
american_scientific_journal-2520	126	6	as	as	ADV
american_scientific_journal-2520	126	7	well	well	ADV
american_scientific_journal-2520	126	8	as	as	ADP
american_scientific_journal-2520	126	9	the	the	DET
american_scientific_journal-2520	126	10	drug	drug	NOUN
american_scientific_journal-2520	126	11	production	production	NOUN
american_scientific_journal-2520	126	12	company	company	NOUN
american_scientific_journal-2520	126	13	to	to	PART
american_scientific_journal-2520	126	14	use	use	VERB
american_scientific_journal-2520	126	15	a	a	DET
american_scientific_journal-2520	126	16	recombinant	recombinant	ADJ
american_scientific_journal-2520	126	17	hpth	hpth	NOUN
american_scientific_journal-2520	126	18	cdna	cdna	PROPN
american_scientific_journal-2520	126	19	or	or	CCONJ
american_scientific_journal-2520	126	20	used	use	VERB
american_scientific_journal-2520	126	21	a	a	DET
american_scientific_journal-2520	126	22	synthetic	synthetic	ADJ
american_scientific_journal-2520	126	23	gene	gene	NOUN
american_scientific_journal-2520	126	24	which	which	PRON
american_scientific_journal-2520	126	25	code	code	VERB
american_scientific_journal-2520	126	26	for	for	ADP
american_scientific_journal-2520	126	27	production	production	NOUN
american_scientific_journal-2520	126	28	of	of	ADP
american_scientific_journal-2520	126	29	the	the	DET
american_scientific_journal-2520	126	30	active	active	ADJ
american_scientific_journal-2520	126	31	hormone	hormone	NOUN
american_scientific_journal-2520	126	32	form	form	NOUN
american_scientific_journal-2520	126	33	(	(	PUNCT
american_scientific_journal-2520	126	34	84	84	NUM
american_scientific_journal-2520	126	35	amino	amino	NOUN
american_scientific_journal-2520	126	36	acid	acid	NOUN
american_scientific_journal-2520	126	37	)	)	PUNCT
american_scientific_journal-2520	126	38	.	.	PUNCT
american_scientific_journal-2520	127	1	for	for	ADP
american_scientific_journal-2520	127	2	the	the	DET
american_scientific_journal-2520	127	3	above	above	ADJ
american_scientific_journal-2520	127	4	reason	reason	NOUN
american_scientific_journal-2520	127	5	a	a	DET
american_scientific_journal-2520	127	6	synthetic	synthetic	ADJ
american_scientific_journal-2520	127	7	recombinant	recombinant	ADJ
american_scientific_journal-2520	127	8	human	human	ADJ
american_scientific_journal-2520	127	9	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	127	10	hormone	hormone	NOUN
american_scientific_journal-2520	127	11	gene	gene	NOUN
american_scientific_journal-2520	127	12	was	be	AUX
american_scientific_journal-2520	127	13	chosen	choose	VERB
american_scientific_journal-2520	127	14	for	for	ADP
american_scientific_journal-2520	127	15	our	our	PRON
american_scientific_journal-2520	127	16	trail	trail	NOUN
american_scientific_journal-2520	127	17	to	to	PART
american_scientific_journal-2520	127	18	produce	produce	VERB
american_scientific_journal-2520	127	19	a	a	DET
american_scientific_journal-2520	127	20	full	full	ADJ
american_scientific_journal-2520	127	21	length	length	NOUN
american_scientific_journal-2520	127	22	active	active	ADJ
american_scientific_journal-2520	127	23	hormone	hormone	NOUN
american_scientific_journal-2520	127	24	(	(	PUNCT
american_scientific_journal-2520	127	25	1	1	NUM
american_scientific_journal-2520	127	26	-	-	NUM
american_scientific_journal-2520	127	27	84	84	NUM
american_scientific_journal-2520	127	28	)	)	PUNCT
american_scientific_journal-2520	127	29	which	which	PRON
american_scientific_journal-2520	127	30	produced	produce	VERB
american_scientific_journal-2520	127	31	by	by	ADP
american_scientific_journal-2520	127	32	using	use	VERB
american_scientific_journal-2520	127	33	five	five	NUM
american_scientific_journal-2520	127	34	long	long	ADJ
american_scientific_journal-2520	127	35	primers	primer	NOUN
american_scientific_journal-2520	127	36	that	that	PRON
american_scientific_journal-2520	127	37	constructed	construct	VERB
american_scientific_journal-2520	127	38	together	together	ADV
american_scientific_journal-2520	127	39	by	by	ADP
american_scientific_journal-2520	127	40	splicing	splice	VERB
american_scientific_journal-2520	127	41	overlapped	overlapped	ADJ
american_scientific_journal-2520	127	42	extension	extension	NOUN
american_scientific_journal-2520	127	43	pcr	pcr	NOUN
american_scientific_journal-2520	127	44	.	.	PUNCT
american_scientific_journal-2520	128	1	the	the	DET
american_scientific_journal-2520	128	2	shpth	shpth	ADJ
american_scientific_journal-2520	128	3	gene	gene	NOUN
american_scientific_journal-2520	128	4	was	be	AUX
american_scientific_journal-2520	128	5	design	design	NOUN
american_scientific_journal-2520	128	6	by	by	ADP
american_scientific_journal-2520	128	7	using	use	VERB
american_scientific_journal-2520	128	8	degenerated	degenerated	ADJ
american_scientific_journal-2520	128	9	codon	codon	NOUN
american_scientific_journal-2520	128	10	,	,	PUNCT
american_scientific_journal-2520	128	11	degenerated	degenerate	VERB
american_scientific_journal-2520	128	12	codon	codon	NOUN
american_scientific_journal-2520	128	13	means	mean	VERB
american_scientific_journal-2520	128	14	multiple	multiple	ADJ
american_scientific_journal-2520	128	15	codons	codon	NOUN
american_scientific_journal-2520	128	16	can	can	AUX
american_scientific_journal-2520	128	17	cod	cod	VERB
american_scientific_journal-2520	128	18	for	for	ADP
american_scientific_journal-2520	128	19	the	the	DET
american_scientific_journal-2520	128	20	same	same	ADJ
american_scientific_journal-2520	128	21	amino	amino	NOUN
american_scientific_journal-2520	128	22	acid	acid	NOUN
american_scientific_journal-2520	129	1	[	[	X
american_scientific_journal-2520	129	2	15	15	NUM
american_scientific_journal-2520	129	3	]	]	PUNCT
american_scientific_journal-2520	129	4	,	,	PUNCT
american_scientific_journal-2520	129	5	e.	e.	PROPN
american_scientific_journal-2520	129	6	coli	coli	PROPN
american_scientific_journal-2520	129	7	-	-	PUNCT
american_scientific_journal-2520	129	8	like	like	ADJ
american_scientific_journal-2520	129	9	’	'	PUNCT
american_scientific_journal-2520	129	10	codons	codon	NOUN
american_scientific_journal-2520	129	11	were	be	AUX
american_scientific_journal-2520	129	12	used	use	VERB
american_scientific_journal-2520	129	13	to	to	PART
american_scientific_journal-2520	129	14	design	design	VERB
american_scientific_journal-2520	129	15	the	the	DET
american_scientific_journal-2520	129	16	recombinant	recombinant	ADJ
american_scientific_journal-2520	129	17	shpth	shpth	ADJ
american_scientific_journal-2520	129	18	gene	gene	NOUN
american_scientific_journal-2520	129	19	that	that	PRON
american_scientific_journal-2520	129	20	’s	’s	AUX
american_scientific_journal-2520	129	21	lead	lead	VERB
american_scientific_journal-2520	129	22	to	to	ADP
american_scientific_journal-2520	129	23	8.3	8.3	NUM
american_scientific_journal-2520	129	24	%	%	NOUN
american_scientific_journal-2520	129	25	of	of	ADP
american_scientific_journal-2520	129	26	the	the	DET
american_scientific_journal-2520	129	27	bases	basis	NOUN
american_scientific_journal-2520	129	28	(	(	PUNCT
american_scientific_journal-2520	129	29	21.4	21.4	NUM
american_scientific_journal-2520	129	30	%	%	NOUN
american_scientific_journal-2520	129	31	of	of	ADP
american_scientific_journal-2520	129	32	the	the	DET
american_scientific_journal-2520	129	33	codons	codon	NOUN
american_scientific_journal-2520	129	34	)	)	PUNCT
american_scientific_journal-2520	129	35	being	be	AUX
american_scientific_journal-2520	129	36	different	different	ADJ
american_scientific_journal-2520	129	37	from	from	ADP
american_scientific_journal-2520	129	38	those	those	PRON
american_scientific_journal-2520	129	39	in	in	ADP
american_scientific_journal-2520	129	40	the	the	DET
american_scientific_journal-2520	129	41	cdna	cdna	PROPN
american_scientific_journal-2520	129	42	of	of	ADP
american_scientific_journal-2520	129	43	human	human	ADJ
american_scientific_journal-2520	129	44	pth	pth	NOUN
american_scientific_journal-2520	130	1	[	[	X
american_scientific_journal-2520	130	2	14	14	NUM
american_scientific_journal-2520	130	3	]	]	PUNCT
american_scientific_journal-2520	130	4	.	.	PUNCT
american_scientific_journal-2520	131	1	in	in	ADP
american_scientific_journal-2520	131	2	unicellular	unicellular	ADJ
american_scientific_journal-2520	131	3	organisms	organism	NOUN
american_scientific_journal-2520	131	4	,	,	PUNCT
american_scientific_journal-2520	131	5	high	high	ADJ
american_scientific_journal-2520	131	6	frequency	frequency	NOUN
american_scientific_journal-2520	131	7	usage	usage	NOUN
american_scientific_journal-2520	131	8	codons	codon	NOUN
american_scientific_journal-2520	131	9	correlate	correlate	VERB
american_scientific_journal-2520	131	10	with	with	ADP
american_scientific_journal-2520	131	11	abundant	abundant	ADJ
american_scientific_journal-2520	131	12	cognate	cognate	ADJ
american_scientific_journal-2520	131	13	trna	trna	NOUN
american_scientific_journal-2520	131	14	molecules	molecule	NOUN
american_scientific_journal-2520	131	15	and	and	CCONJ
american_scientific_journal-2520	131	16	have	have	AUX
american_scientific_journal-2520	131	17	evolved	evolve	VERB
american_scientific_journal-2520	131	18	to	to	PART
american_scientific_journal-2520	131	19	optimize	optimize	VERB
american_scientific_journal-2520	131	20	translational	translational	ADJ
american_scientific_journal-2520	131	21	efficiency	efficiency	NOUN
american_scientific_journal-2520	131	22	[	[	X
american_scientific_journal-2520	131	23	16	16	NUM
american_scientific_journal-2520	131	24	]	]	PUNCT
american_scientific_journal-2520	131	25	,	,	PUNCT
american_scientific_journal-2520	131	26	therefore	therefore	ADV
american_scientific_journal-2520	131	27	the	the	DET
american_scientific_journal-2520	131	28	more	more	ADV
american_scientific_journal-2520	131	29	preferred	preferred	ADJ
american_scientific_journal-2520	131	30	codon	codon	NOUN
american_scientific_journal-2520	131	31	for	for	ADP
american_scientific_journal-2520	131	32	bacteria	bacteria	NOUN
american_scientific_journal-2520	131	33	were	be	AUX
american_scientific_journal-2520	131	34	used	use	VERB
american_scientific_journal-2520	131	35	in	in	ADP
american_scientific_journal-2520	131	36	order	order	NOUN
american_scientific_journal-2520	131	37	to	to	PART
american_scientific_journal-2520	131	38	increase	increase	VERB
american_scientific_journal-2520	131	39	the	the	DET
american_scientific_journal-2520	131	40	recombinant	recombinant	ADJ
american_scientific_journal-2520	131	41	hpth	hpth	NOUN
american_scientific_journal-2520	131	42	expression	expression	NOUN
american_scientific_journal-2520	131	43	.	.	PUNCT
american_scientific_journal-2520	132	1	the	the	DET
american_scientific_journal-2520	132	2	shpth	shpth	ADJ
american_scientific_journal-2520	132	3	gene	gene	NOUN
american_scientific_journal-2520	132	4	was	be	AUX
american_scientific_journal-2520	132	5	constructed	construct	VERB
american_scientific_journal-2520	132	6	by	by	ADP
american_scientific_journal-2520	132	7	chemically	chemically	ADV
american_scientific_journal-2520	132	8	synthesis	synthesis	VERB
american_scientific_journal-2520	132	9	five	five	NUM
american_scientific_journal-2520	132	10	long	long	ADJ
american_scientific_journal-2520	132	11	oligonucleotides	oligonucleotide	NOUN
american_scientific_journal-2520	132	12	using	use	VERB
american_scientific_journal-2520	132	13	splicing	splicing	NOUN
american_scientific_journal-2520	132	14	overlap	overlap	NOUN
american_scientific_journal-2520	132	15	extension	extension	NOUN
american_scientific_journal-2520	132	16	pcr	pcr	NOUN
american_scientific_journal-2520	132	17	method	method	NOUN
american_scientific_journal-2520	132	18	,	,	PUNCT
american_scientific_journal-2520	132	19	in	in	ADP
american_scientific_journal-2520	132	20	this	this	DET
american_scientific_journal-2520	132	21	method	method	NOUN
american_scientific_journal-2520	132	22	initial	initial	ADJ
american_scientific_journal-2520	132	23	pcrs	pcrs	NOUN
american_scientific_journal-2520	132	24	generate	generate	VERB
american_scientific_journal-2520	132	25	overlapping	overlap	VERB
american_scientific_journal-2520	132	26	gene	gene	NOUN
american_scientific_journal-2520	132	27	segments	segment	NOUN
american_scientific_journal-2520	132	28	that	that	PRON
american_scientific_journal-2520	132	29	are	be	AUX
american_scientific_journal-2520	132	30	then	then	ADV
american_scientific_journal-2520	132	31	used	use	VERB
american_scientific_journal-2520	132	32	as	as	ADP
american_scientific_journal-2520	132	33	template	template	NOUN
american_scientific_journal-2520	132	34	dna	dna	NOUN
american_scientific_journal-2520	132	35	for	for	ADP
american_scientific_journal-2520	132	36	another	another	DET
american_scientific_journal-2520	132	37	pcr	pcr	NOUN
american_scientific_journal-2520	132	38	to	to	PART
american_scientific_journal-2520	132	39	create	create	VERB
american_scientific_journal-2520	132	40	a	a	DET
american_scientific_journal-2520	132	41	full	full	ADJ
american_scientific_journal-2520	132	42	-	-	PUNCT
american_scientific_journal-2520	132	43	length	length	NOUN
american_scientific_journal-2520	132	44	product	product	NOUN
american_scientific_journal-2520	132	45	[	[	X
american_scientific_journal-2520	132	46	11	11	NUM
american_scientific_journal-2520	132	47	]	]	PUNCT
american_scientific_journal-2520	132	48	,	,	PUNCT
american_scientific_journal-2520	132	49	chemical	chemical	ADJ
american_scientific_journal-2520	132	50	synthesis	synthesis	NOUN
american_scientific_journal-2520	132	51	of	of	ADP
american_scientific_journal-2520	132	52	nucleotide	nucleotide	ADJ
american_scientific_journal-2520	132	53	sequences	sequence	NOUN
american_scientific_journal-2520	132	54	can	can	AUX
american_scientific_journal-2520	132	55	deliver	deliver	VERB
american_scientific_journal-2520	132	56	a	a	DET
american_scientific_journal-2520	132	57	powerful	powerful	ADJ
american_scientific_journal-2520	132	58	molecular	molecular	ADJ
american_scientific_journal-2520	132	59	means	mean	NOUN
american_scientific_journal-2520	132	60	for	for	ADP
american_scientific_journal-2520	132	61	genes	gene	NOUN
american_scientific_journal-2520	132	62	modification	modification	NOUN
american_scientific_journal-2520	132	63	,	,	PUNCT
american_scientific_journal-2520	132	64	revealing	reveal	VERB
american_scientific_journal-2520	132	65	gene	gene	NOUN
american_scientific_journal-2520	132	66	functions	function	NOUN
american_scientific_journal-2520	132	67	and	and	CCONJ
american_scientific_journal-2520	132	68	studying	study	VERB
american_scientific_journal-2520	132	69	the	the	DET
american_scientific_journal-2520	132	70	relationship	relationship	NOUN
american_scientific_journal-2520	132	71	between	between	ADP
american_scientific_journal-2520	132	72	the	the	DET
american_scientific_journal-2520	132	73	structure	structure	NOUN
american_scientific_journal-2520	132	74	of	of	ADP
american_scientific_journal-2520	132	75	each	each	DET
american_scientific_journal-2520	132	76	protein	protein	NOUN
american_scientific_journal-2520	132	77	part	part	NOUN
american_scientific_journal-2520	132	78	and	and	CCONJ
american_scientific_journal-2520	132	79	its	its	PRON
american_scientific_journal-2520	132	80	function	function	NOUN
american_scientific_journal-2520	132	81	[	[	X
american_scientific_journal-2520	132	82	17	17	NUM
american_scientific_journal-2520	132	83	]	]	PUNCT
american_scientific_journal-2520	132	84	.	.	PUNCT
american_scientific_journal-2520	133	1	american	american	PROPN
american_scientific_journal-2520	133	2	scientific	scientific	ADJ
american_scientific_journal-2520	133	3	research	research	PROPN
american_scientific_journal-2520	133	4	journal	journal	NOUN
american_scientific_journal-2520	133	5	for	for	ADP
american_scientific_journal-2520	133	6	engineering	engineering	NOUN
american_scientific_journal-2520	133	7	,	,	PUNCT
american_scientific_journal-2520	133	8	technology	technology	NOUN
american_scientific_journal-2520	133	9	,	,	PUNCT
american_scientific_journal-2520	133	10	and	and	CCONJ
american_scientific_journal-2520	133	11	sciences	science	NOUN
american_scientific_journal-2520	133	12	(	(	PUNCT
american_scientific_journal-2520	133	13	asrjets	asrjet	NOUN
american_scientific_journal-2520	133	14	)	)	PUNCT
american_scientific_journal-2520	133	15	(	(	PUNCT
american_scientific_journal-2520	133	16	2017	2017	NUM
american_scientific_journal-2520	133	17	)	)	PUNCT
american_scientific_journal-2520	133	18	volume	volume	NOUN
american_scientific_journal-2520	133	19	27	27	NUM
american_scientific_journal-2520	133	20	,	,	PUNCT
american_scientific_journal-2520	133	21	no	no	DET
american_scientific_journal-2520	133	22	1	1	NUM
american_scientific_journal-2520	133	23	,	,	PUNCT
american_scientific_journal-2520	133	24	pp	pp	ADV
american_scientific_journal-2520	133	25	12	12	NUM
american_scientific_journal-2520	133	26	-	-	SYM
american_scientific_journal-2520	133	27	24	24	NUM
american_scientific_journal-2520	133	28	21	21	NUM
american_scientific_journal-2520	133	29	also	also	ADV
american_scientific_journal-2520	133	30	,	,	PUNCT
american_scientific_journal-2520	133	31	the	the	DET
american_scientific_journal-2520	133	32	shpth	shpth	ADJ
american_scientific_journal-2520	133	33	gene	gene	NOUN
american_scientific_journal-2520	133	34	was	be	AUX
american_scientific_journal-2520	133	35	constructed	construct	VERB
american_scientific_journal-2520	133	36	with	with	ADP
american_scientific_journal-2520	133	37	a	a	DET
american_scientific_journal-2520	133	38	two	two	NUM
american_scientific_journal-2520	133	39	restriction	restriction	NOUN
american_scientific_journal-2520	133	40	sites	site	NOUN
american_scientific_journal-2520	133	41	,	,	PUNCT
american_scientific_journal-2520	133	42	the	the	DET
american_scientific_journal-2520	133	43	restriction	restriction	NOUN
american_scientific_journal-2520	133	44	sites	site	NOUN
american_scientific_journal-2520	133	45	in	in	ADP
american_scientific_journal-2520	133	46	the	the	DET
american_scientific_journal-2520	133	47	start	start	NOUN
american_scientific_journal-2520	133	48	and	and	CCONJ
american_scientific_journal-2520	133	49	the	the	DET
american_scientific_journal-2520	133	50	end	end	NOUN
american_scientific_journal-2520	133	51	of	of	ADP
american_scientific_journal-2520	133	52	gene	gene	NOUN
american_scientific_journal-2520	133	53	(	(	PUNCT
american_scientific_journal-2520	133	54	kpni	kpni	VERB
american_scientific_journal-2520	133	55	in	in	ADP
american_scientific_journal-2520	133	56	3	3	NUM
american_scientific_journal-2520	133	57	`	`	PUNCT
american_scientific_journal-2520	133	58	end	end	NOUN
american_scientific_journal-2520	133	59	of	of	ADP
american_scientific_journal-2520	133	60	the	the	DET
american_scientific_journal-2520	133	61	gene	gene	NOUN
american_scientific_journal-2520	133	62	and	and	CCONJ
american_scientific_journal-2520	133	63	hindiii	hindiii	NOUN
american_scientific_journal-2520	133	64	in	in	ADP
american_scientific_journal-2520	133	65	5`end	5`end	NUM
american_scientific_journal-2520	133	66	)	)	PUNCT
american_scientific_journal-2520	133	67	,	,	PUNCT
american_scientific_journal-2520	133	68	both	both	PRON
american_scientific_journal-2520	133	69	of	of	ADP
american_scientific_journal-2520	133	70	these	these	DET
american_scientific_journal-2520	133	71	restriction	restriction	NOUN
american_scientific_journal-2520	133	72	-	-	PUNCT
american_scientific_journal-2520	133	73	enzyme	enzyme	NOUN
american_scientific_journal-2520	133	74	cut	cut	NOUN
american_scientific_journal-2520	133	75	the	the	DET
american_scientific_journal-2520	133	76	double	double	ADJ
american_scientific_journal-2520	133	77	stranded	strand	VERB
american_scientific_journal-2520	133	78	dna	dna	NOUN
american_scientific_journal-2520	133	79	to	to	PART
american_scientific_journal-2520	133	80	form	form	VERB
american_scientific_journal-2520	133	81	a	a	DET
american_scientific_journal-2520	133	82	sticky	sticky	ADJ
american_scientific_journal-2520	133	83	end	end	NOUN
american_scientific_journal-2520	133	84	.	.	PUNCT
american_scientific_journal-2520	134	1	sticky	sticky	ADJ
american_scientific_journal-2520	134	2	ends	end	NOUN
american_scientific_journal-2520	134	3	are	be	AUX
american_scientific_journal-2520	134	4	more	more	ADV
american_scientific_journal-2520	134	5	useful	useful	ADJ
american_scientific_journal-2520	134	6	in	in	ADP
american_scientific_journal-2520	134	7	molecular	molecular	ADJ
american_scientific_journal-2520	134	8	cloning	cloning	NOUN
american_scientific_journal-2520	134	9	because	because	SCONJ
american_scientific_journal-2520	134	10	they	they	PRON
american_scientific_journal-2520	134	11	ensure	ensure	VERB
american_scientific_journal-2520	134	12	that	that	SCONJ
american_scientific_journal-2520	134	13	the	the	DET
american_scientific_journal-2520	134	14	gene	gene	NOUN
american_scientific_journal-2520	134	15	fragment	fragment	NOUN
american_scientific_journal-2520	134	16	is	be	AUX
american_scientific_journal-2520	134	17	inserted	insert	VERB
american_scientific_journal-2520	134	18	into	into	ADP
american_scientific_journal-2520	134	19	the	the	DET
american_scientific_journal-2520	134	20	plasmid	plasmid	NOUN
american_scientific_journal-2520	134	21	in	in	ADP
american_scientific_journal-2520	134	22	the	the	DET
american_scientific_journal-2520	134	23	right	right	ADJ
american_scientific_journal-2520	134	24	direction	direction	NOUN
american_scientific_journal-2520	134	25	[	[	X
american_scientific_journal-2520	134	26	18	18	NUM
american_scientific_journal-2520	134	27	]	]	PUNCT
american_scientific_journal-2520	134	28	.	.	PUNCT
american_scientific_journal-2520	135	1	as	as	ADV
american_scientific_journal-2520	135	2	well	well	ADV
american_scientific_journal-2520	135	3	as	as	ADP
american_scientific_journal-2520	135	4	the	the	DET
american_scientific_journal-2520	135	5	sites	site	NOUN
american_scientific_journal-2520	135	6	of	of	ADP
american_scientific_journal-2520	135	7	restriction	restriction	NOUN
american_scientific_journal-2520	135	8	enzyme	enzyme	NOUN
american_scientific_journal-2520	135	9	,	,	PUNCT
american_scientific_journal-2520	135	10	the	the	DET
american_scientific_journal-2520	135	11	shpth	shpth	ADJ
american_scientific_journal-2520	135	12	gene	gene	NOUN
american_scientific_journal-2520	135	13	contain	contain	VERB
american_scientific_journal-2520	135	14	a	a	DET
american_scientific_journal-2520	135	15	stop	stop	NOUN
american_scientific_journal-2520	135	16	codon	codon	NOUN
american_scientific_journal-2520	135	17	before	before	ADP
american_scientific_journal-2520	135	18	hindiii	hindiii	PROPN
american_scientific_journal-2520	135	19	restriction	restriction	NOUN
american_scientific_journal-2520	135	20	site	site	NOUN
american_scientific_journal-2520	135	21	at	at	ADP
american_scientific_journal-2520	135	22	5`end	5`end	NUM
american_scientific_journal-2520	135	23	,	,	PUNCT
american_scientific_journal-2520	135	24	the	the	DET
american_scientific_journal-2520	135	25	stop	stop	NOUN
american_scientific_journal-2520	135	26	codon	codon	NOUN
american_scientific_journal-2520	135	27	is	be	AUX
american_scientific_journal-2520	135	28	a	a	DET
american_scientific_journal-2520	135	29	nucleotide	nucleotide	ADJ
american_scientific_journal-2520	135	30	triplet	triplet	NOUN
american_scientific_journal-2520	135	31	within	within	ADP
american_scientific_journal-2520	135	32	messenger	messenger	NOUN
american_scientific_journal-2520	135	33	rna	rna	NOUN
american_scientific_journal-2520	135	34	that	that	PRON
american_scientific_journal-2520	135	35	signals	signal	VERB
american_scientific_journal-2520	135	36	a	a	DET
american_scientific_journal-2520	135	37	termination	termination	NOUN
american_scientific_journal-2520	135	38	of	of	ADP
american_scientific_journal-2520	135	39	translation	translation	NOUN
american_scientific_journal-2520	135	40	of	of	ADP
american_scientific_journal-2520	135	41	the	the	DET
american_scientific_journal-2520	135	42	proteins	protein	NOUN
american_scientific_journal-2520	135	43	[	[	X
american_scientific_journal-2520	135	44	19	19	NUM
american_scientific_journal-2520	135	45	]	]	PUNCT
american_scientific_journal-2520	135	46	.	.	PUNCT
american_scientific_journal-2520	136	1	the	the	DET
american_scientific_journal-2520	136	2	expression	expression	NOUN
american_scientific_journal-2520	136	3	of	of	ADP
american_scientific_journal-2520	136	4	recombinant	recombinant	ADJ
american_scientific_journal-2520	136	5	human	human	ADJ
american_scientific_journal-2520	136	6	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	136	7	hormone	hormone	NOUN
american_scientific_journal-2520	136	8	was	be	AUX
american_scientific_journal-2520	136	9	carried	carry	VERB
american_scientific_journal-2520	136	10	out	out	ADP
american_scientific_journal-2520	136	11	by	by	ADP
american_scientific_journal-2520	136	12	growing	grow	VERB
american_scientific_journal-2520	136	13	the	the	DET
american_scientific_journal-2520	136	14	transformed	transform	VERB
american_scientific_journal-2520	136	15	isolate	isolate	NOUN
american_scientific_journal-2520	136	16	bl21(de3	bl21(de3	VERB
american_scientific_journal-2520	136	17	)	)	PUNCT
american_scientific_journal-2520	136	18	in	in	ADP
american_scientific_journal-2520	136	19	the	the	DET
american_scientific_journal-2520	136	20	lb	lb	NOUN
american_scientific_journal-2520	136	21	medium	medium	NOUN
american_scientific_journal-2520	136	22	because	because	SCONJ
american_scientific_journal-2520	136	23	the	the	DET
american_scientific_journal-2520	136	24	bl21	bl21	PROPN
american_scientific_journal-2520	136	25	(	(	PUNCT
american_scientific_journal-2520	136	26	de3	de3	NOUN
american_scientific_journal-2520	136	27	)	)	PUNCT
american_scientific_journal-2520	136	28	isolate	isolate	NOUN
american_scientific_journal-2520	136	29	was	be	AUX
american_scientific_journal-2520	136	30	used	use	VERB
american_scientific_journal-2520	136	31	for	for	ADP
american_scientific_journal-2520	136	32	gene	gene	NOUN
american_scientific_journal-2520	136	33	expression	expression	NOUN
american_scientific_journal-2520	136	34	because	because	SCONJ
american_scientific_journal-2520	136	35	it	it	PRON
american_scientific_journal-2520	136	36	's	be	AUX
american_scientific_journal-2520	136	37	a	a	DET
american_scientific_journal-2520	136	38	lysogen	lysogen	NOUN
american_scientific_journal-2520	136	39	of	of	ADP
american_scientific_journal-2520	136	40	λde3	λde3	PROPN
american_scientific_journal-2520	136	41	,	,	PUNCT
american_scientific_journal-2520	136	42	and	and	CCONJ
american_scientific_journal-2520	136	43	therefore	therefore	ADV
american_scientific_journal-2520	136	44	carries	carry	VERB
american_scientific_journal-2520	136	45	a	a	DET
american_scientific_journal-2520	136	46	chromosomal	chromosomal	ADJ
american_scientific_journal-2520	136	47	copy	copy	NOUN
american_scientific_journal-2520	136	48	of	of	ADP
american_scientific_journal-2520	136	49	the	the	DET
american_scientific_journal-2520	136	50	t7	t7	PROPN
american_scientific_journal-2520	136	51	rna	rna	PROPN
american_scientific_journal-2520	136	52	polymerase	polymerase	NOUN
american_scientific_journal-2520	136	53	gene	gene	NOUN
american_scientific_journal-2520	136	54	under	under	ADP
american_scientific_journal-2520	136	55	control	control	NOUN
american_scientific_journal-2520	136	56	of	of	ADP
american_scientific_journal-2520	136	57	the	the	DET
american_scientific_journal-2520	136	58	lacuv5	lacuv5	NOUN
american_scientific_journal-2520	136	59	promoter	promoter	NOUN
american_scientific_journal-2520	136	60	such	such	ADJ
american_scientific_journal-2520	136	61	strains	strain	NOUN
american_scientific_journal-2520	136	62	are	be	AUX
american_scientific_journal-2520	136	63	suitable	suitable	ADJ
american_scientific_journal-2520	136	64	for	for	ADP
american_scientific_journal-2520	136	65	production	production	NOUN
american_scientific_journal-2520	136	66	of	of	ADP
american_scientific_journal-2520	136	67	protein	protein	NOUN
american_scientific_journal-2520	136	68	from	from	ADP
american_scientific_journal-2520	136	69	target	target	NOUN
american_scientific_journal-2520	136	70	genes	gene	NOUN
american_scientific_journal-2520	136	71	cloned	clone	VERB
american_scientific_journal-2520	136	72	in	in	ADP
american_scientific_journal-2520	136	73	pet	pet	ADJ
american_scientific_journal-2520	136	74	vectors	vector	NOUN
american_scientific_journal-2520	136	75	or	or	CCONJ
american_scientific_journal-2520	136	76	other	other	ADJ
american_scientific_journal-2520	136	77	protein	protein	NOUN
american_scientific_journal-2520	136	78	under	under	ADP
american_scientific_journal-2520	136	79	the	the	DET
american_scientific_journal-2520	136	80	control	control	NOUN
american_scientific_journal-2520	136	81	of	of	ADP
american_scientific_journal-2520	136	82	t7	t7	PROPN
american_scientific_journal-2520	136	83	promoter	promoter	NOUN
american_scientific_journal-2520	137	1	[	[	X
american_scientific_journal-2520	137	2	20	20	NUM
american_scientific_journal-2520	137	3	]	]	PUNCT
american_scientific_journal-2520	137	4	,	,	PUNCT
american_scientific_journal-2520	137	5	while	while	SCONJ
american_scientific_journal-2520	137	6	there	there	PRON
american_scientific_journal-2520	137	7	was	be	VERB
american_scientific_journal-2520	137	8	no	no	DET
american_scientific_journal-2520	137	9	ability	ability	NOUN
american_scientific_journal-2520	137	10	for	for	ADP
american_scientific_journal-2520	137	11	using	use	VERB
american_scientific_journal-2520	137	12	the	the	DET
american_scientific_journal-2520	137	13	other	other	ADJ
american_scientific_journal-2520	137	14	transformed	transform	VERB
american_scientific_journal-2520	137	15	isolate	isolate	NOUN
american_scientific_journal-2520	137	16	(	(	PUNCT
american_scientific_journal-2520	137	17	dh5α	dh5α	PROPN
american_scientific_journal-2520	137	18	)	)	PUNCT
american_scientific_journal-2520	137	19	for	for	ADP
american_scientific_journal-2520	137	20	the	the	DET
american_scientific_journal-2520	137	21	production	production	NOUN
american_scientific_journal-2520	137	22	that	that	PRON
american_scientific_journal-2520	137	23	’s	’	VERB
american_scientific_journal-2520	137	24	because	because	SCONJ
american_scientific_journal-2520	137	25	the	the	DET
american_scientific_journal-2520	137	26	genotype	genotype	NOUN
american_scientific_journal-2520	137	27	of	of	ADP
american_scientific_journal-2520	137	28	dh5	dh5	PROPN
american_scientific_journal-2520	137	29	α	α	PRON
american_scientific_journal-2520	137	30	made	make	VERB
american_scientific_journal-2520	137	31	it	it	PRON
american_scientific_journal-2520	137	32	a	a	DET
american_scientific_journal-2520	137	33	suitable	suitable	ADJ
american_scientific_journal-2520	137	34	strain	strain	NOUN
american_scientific_journal-2520	137	35	for	for	ADP
american_scientific_journal-2520	137	36	plasmid	plasmid	NOUN
american_scientific_journal-2520	137	37	maintenance	maintenance	NOUN
american_scientific_journal-2520	137	38	and	and	CCONJ
american_scientific_journal-2520	137	39	growth	growth	NOUN
american_scientific_journal-2520	137	40	without	without	ADP
american_scientific_journal-2520	137	41	modification	modification	NOUN
american_scientific_journal-2520	137	42	because	because	SCONJ
american_scientific_journal-2520	137	43	it	it	PRON
american_scientific_journal-2520	137	44	has	have	VERB
american_scientific_journal-2520	137	45	reca	reca	NOUN
american_scientific_journal-2520	137	46	mutation	mutation	NOUN
american_scientific_journal-2520	137	47	and	and	CCONJ
american_scientific_journal-2520	137	48	lacks	lack	VERB
american_scientific_journal-2520	137	49	some	some	DET
american_scientific_journal-2520	137	50	endonucleases	endonuclease	NOUN
american_scientific_journal-2520	137	51	which	which	PRON
american_scientific_journal-2520	137	52	might	might	AUX
american_scientific_journal-2520	137	53	digest	digest	VERB
american_scientific_journal-2520	137	54	the	the	DET
american_scientific_journal-2520	137	55	plasmids	plasmid	NOUN
american_scientific_journal-2520	137	56	during	during	ADP
american_scientific_journal-2520	137	57	the	the	DET
american_scientific_journal-2520	137	58	isolation	isolation	NOUN
american_scientific_journal-2520	137	59	procedure	procedure	NOUN
american_scientific_journal-2520	137	60	,	,	PUNCT
american_scientific_journal-2520	137	61	the	the	DET
american_scientific_journal-2520	137	62	dh5α	dh5α	PROPN
american_scientific_journal-2520	137	63	strain	strain	NOUN
american_scientific_journal-2520	137	64	has	have	VERB
american_scientific_journal-2520	137	65	a	a	DET
american_scientific_journal-2520	137	66	mutations	mutation	NOUN
american_scientific_journal-2520	137	67	in	in	ADP
american_scientific_journal-2520	137	68	its	its	PRON
american_scientific_journal-2520	137	69	genotype	genotype	NOUN
american_scientific_journal-2520	137	70	make	make	VERB
american_scientific_journal-2520	137	71	it	it	PRON
american_scientific_journal-2520	137	72	a	a	DET
american_scientific_journal-2520	137	73	best	good	ADJ
american_scientific_journal-2520	137	74	strain	strain	NOUN
american_scientific_journal-2520	137	75	in	in	ADP
american_scientific_journal-2520	137	76	laboratory	laboratory	NOUN
american_scientific_journal-2520	137	77	cloning	cloning	NOUN
american_scientific_journal-2520	137	78	procedures	procedure	NOUN
american_scientific_journal-2520	137	79	[	[	X
american_scientific_journal-2520	137	80	21	21	NUM
american_scientific_journal-2520	137	81	]	]	PUNCT
american_scientific_journal-2520	137	82	,	,	PUNCT
american_scientific_journal-2520	137	83	also	also	ADV
american_scientific_journal-2520	137	84	the	the	DET
american_scientific_journal-2520	137	85	dh5α	dh5α	PROPN
american_scientific_journal-2520	137	86	isolate	isolate	NOUN
american_scientific_journal-2520	137	87	do	do	AUX
american_scientific_journal-2520	137	88	not	not	PART
american_scientific_journal-2520	137	89	have	have	VERB
american_scientific_journal-2520	137	90	t7	t7	PROPN
american_scientific_journal-2520	137	91	rna	rna	NOUN
american_scientific_journal-2520	137	92	polymerase	polymerase	NOUN
american_scientific_journal-2520	137	93	on	on	ADP
american_scientific_journal-2520	137	94	its	its	PRON
american_scientific_journal-2520	137	95	genomic	genomic	ADJ
american_scientific_journal-2520	137	96	dna	dna	NOUN
american_scientific_journal-2520	137	97	therefore	therefore	ADV
american_scientific_journal-2520	137	98	it	it	PRON
american_scientific_journal-2520	137	99	can	can	AUX
american_scientific_journal-2520	137	100	not	not	PART
american_scientific_journal-2520	137	101	express	express	VERB
american_scientific_journal-2520	137	102	the	the	DET
american_scientific_journal-2520	137	103	target	target	NOUN
american_scientific_journal-2520	137	104	gene	gene	NOUN
american_scientific_journal-2520	137	105	which	which	PRON
american_scientific_journal-2520	137	106	express	express	VERB
american_scientific_journal-2520	137	107	under	under	ADP
american_scientific_journal-2520	137	108	the	the	DET
american_scientific_journal-2520	137	109	control	control	NOUN
american_scientific_journal-2520	137	110	of	of	ADP
american_scientific_journal-2520	137	111	t7	t7	PROPN
american_scientific_journal-2520	137	112	promoter	promoter	NOUN
american_scientific_journal-2520	137	113	,	,	PUNCT
american_scientific_journal-2520	137	114	the	the	DET
american_scientific_journal-2520	137	115	latter	latter	ADJ
american_scientific_journal-2520	137	116	have	have	VERB
american_scientific_journal-2520	137	117	only	only	ADV
american_scientific_journal-2520	137	118	the	the	DET
american_scientific_journal-2520	137	119	ability	ability	NOUN
american_scientific_journal-2520	137	120	to	to	PART
american_scientific_journal-2520	137	121	bind	bind	VERB
american_scientific_journal-2520	137	122	with	with	ADP
american_scientific_journal-2520	137	123	t7	t7	PROPN
american_scientific_journal-2520	137	124	rna	rna	PROPN
american_scientific_journal-2520	137	125	polymerase	polymerase	NOUN
american_scientific_journal-2520	137	126	and	and	CCONJ
american_scientific_journal-2520	137	127	not	not	PART
american_scientific_journal-2520	137	128	any	any	DET
american_scientific_journal-2520	137	129	other	other	ADJ
american_scientific_journal-2520	137	130	rna	rna	NOUN
american_scientific_journal-2520	137	131	polymerase	polymerase	NOUN
american_scientific_journal-2520	137	132	.	.	PUNCT
american_scientific_journal-2520	138	1	in	in	ADP
american_scientific_journal-2520	138	2	order	order	NOUN
american_scientific_journal-2520	138	3	to	to	PART
american_scientific_journal-2520	138	4	get	get	VERB
american_scientific_journal-2520	138	5	the	the	DET
american_scientific_journal-2520	138	6	mrna	mrna	NOUN
american_scientific_journal-2520	138	7	of	of	ADP
american_scientific_journal-2520	138	8	the	the	DET
american_scientific_journal-2520	138	9	cloning	cloning	NOUN
american_scientific_journal-2520	138	10	gene	gene	NOUN
american_scientific_journal-2520	138	11	.	.	PUNCT
american_scientific_journal-2520	139	1	plasmid	plasmid	NOUN
american_scientific_journal-2520	139	2	in	in	ADP
american_scientific_journal-2520	139	3	the	the	DET
american_scientific_journal-2520	139	4	transformed	transform	VERB
american_scientific_journal-2520	139	5	bacterial	bacterial	ADJ
american_scientific_journal-2520	139	6	isolate	isolate	NOUN
american_scientific_journal-2520	139	7	must	must	AUX
american_scientific_journal-2520	139	8	induced	induce	VERB
american_scientific_journal-2520	139	9	for	for	ADP
american_scientific_journal-2520	139	10	mrna	mrna	PROPN
american_scientific_journal-2520	139	11	production	production	NOUN
american_scientific_journal-2520	139	12	by	by	ADP
american_scientific_journal-2520	139	13	addition	addition	NOUN
american_scientific_journal-2520	139	14	of	of	ADP
american_scientific_journal-2520	139	15	iptg	iptg	NOUN
american_scientific_journal-2520	139	16	to	to	ADP
american_scientific_journal-2520	139	17	the	the	DET
american_scientific_journal-2520	139	18	bacterial	bacterial	ADJ
american_scientific_journal-2520	139	19	broth	broth	NOUN
american_scientific_journal-2520	139	20	and	and	CCONJ
american_scientific_journal-2520	139	21	incubates	incubate	VERB
american_scientific_journal-2520	139	22	it	it	PRON
american_scientific_journal-2520	139	23	for	for	ADP
american_scientific_journal-2520	139	24	several	several	ADJ
american_scientific_journal-2520	139	25	hours	hour	NOUN
american_scientific_journal-2520	139	26	4	4	NUM
american_scientific_journal-2520	139	27	-	-	SYM
american_scientific_journal-2520	139	28	5	5	NUM
american_scientific_journal-2520	139	29	hours	hour	NOUN
american_scientific_journal-2520	139	30	at	at	ADP
american_scientific_journal-2520	139	31	2530c	2530c	NOUN
american_scientific_journal-2520	139	32	°	°	NOUN
american_scientific_journal-2520	139	33	,	,	PUNCT
american_scientific_journal-2520	139	34	at	at	ADP
american_scientific_journal-2520	139	35	that	that	DET
american_scientific_journal-2520	139	36	time	time	NOUN
american_scientific_journal-2520	139	37	the	the	DET
american_scientific_journal-2520	139	38	lac	lac	X
american_scientific_journal-2520	139	39	i	i	PRON
american_scientific_journal-2520	139	40	in	in	ADP
american_scientific_journal-2520	139	41	both	both	CCONJ
american_scientific_journal-2520	139	42	the	the	DET
american_scientific_journal-2520	139	43	plasmid	plasmid	ADJ
american_scientific_journal-2520	139	44	and	and	CCONJ
american_scientific_journal-2520	139	45	genomic	genomic	ADJ
american_scientific_journal-2520	139	46	dna	dna	PROPN
american_scientific_journal-2520	139	47	was	be	AUX
american_scientific_journal-2520	139	48	repressed	repress	VERB
american_scientific_journal-2520	139	49	by	by	ADP
american_scientific_journal-2520	139	50	binding	bind	VERB
american_scientific_journal-2520	139	51	with	with	ADP
american_scientific_journal-2520	139	52	iptg	iptg	NOUN
american_scientific_journal-2520	139	53	molecules	molecule	NOUN
american_scientific_journal-2520	139	54	that	that	PRON
american_scientific_journal-2520	139	55	lead	lead	VERB
american_scientific_journal-2520	139	56	to	to	ADP
american_scientific_journal-2520	139	57	t7rna	t7rna	ADJ
american_scientific_journal-2520	139	58	polymerase	polymerase	NOUN
american_scientific_journal-2520	139	59	expression	expression	NOUN
american_scientific_journal-2520	139	60	in	in	ADP
american_scientific_journal-2520	139	61	the	the	DET
american_scientific_journal-2520	139	62	genomic	genomic	ADJ
american_scientific_journal-2520	139	63	dna	dna	NOUN
american_scientific_journal-2520	139	64	of	of	ADP
american_scientific_journal-2520	139	65	the	the	DET
american_scientific_journal-2520	139	66	bacterial	bacterial	ADJ
american_scientific_journal-2520	139	67	and	and	CCONJ
american_scientific_journal-2520	139	68	bind	bind	VERB
american_scientific_journal-2520	139	69	to	to	ADP
american_scientific_journal-2520	139	70	t7	t7	PROPN
american_scientific_journal-2520	139	71	promoter	promoter	NOUN
american_scientific_journal-2520	139	72	in	in	ADP
american_scientific_journal-2520	139	73	the	the	DET
american_scientific_journal-2520	139	74	pet32a	pet32a	NOUN
american_scientific_journal-2520	139	75	-	-	ADJ
american_scientific_journal-2520	139	76	shpth	shpth	ADJ
american_scientific_journal-2520	139	77	plasmid	plasmid	NOUN
american_scientific_journal-2520	139	78	,	,	PUNCT
american_scientific_journal-2520	139	79	that	that	PRON
american_scientific_journal-2520	139	80	permit	permit	NOUN
american_scientific_journal-2520	139	81	to	to	ADP
american_scientific_journal-2520	139	82	the	the	DET
american_scientific_journal-2520	139	83	target	target	NOUN
american_scientific_journal-2520	139	84	gene	gene	NOUN
american_scientific_journal-2520	139	85	to	to	ADP
american_scientific_journal-2520	139	86	transcribed	transcribed	NOUN
american_scientific_journal-2520	139	87	and	and	CCONJ
american_scientific_journal-2520	139	88	form	form	PROPN
american_scientific_journal-2520	139	89	mrna	mrna	PROPN
american_scientific_journal-2520	139	90	which	which	PRON
american_scientific_journal-2520	139	91	translate	translate	VERB
american_scientific_journal-2520	139	92	to	to	PART
american_scientific_journal-2520	139	93	form	form	VERB
american_scientific_journal-2520	139	94	the	the	DET
american_scientific_journal-2520	139	95	target	target	NOUN
american_scientific_journal-2520	139	96	protein	protein	NOUN
american_scientific_journal-2520	140	1	[	[	X
american_scientific_journal-2520	140	2	22	22	NUM
american_scientific_journal-2520	140	3	]	]	PUNCT
american_scientific_journal-2520	140	4	.	.	PUNCT
american_scientific_journal-2520	141	1	at	at	ADP
american_scientific_journal-2520	141	2	this	this	DET
american_scientific_journal-2520	141	3	point	point	NOUN
american_scientific_journal-2520	141	4	the	the	DET
american_scientific_journal-2520	141	5	total	total	ADJ
american_scientific_journal-2520	141	6	bacterial	bacterial	ADJ
american_scientific_journal-2520	141	7	rna	rna	NOUN
american_scientific_journal-2520	141	8	was	be	AUX
american_scientific_journal-2520	141	9	extracted	extract	VERB
american_scientific_journal-2520	141	10	by	by	ADP
american_scientific_journal-2520	141	11	trizol	trizol	ADJ
american_scientific_journal-2520	141	12	reagent	reagent	NOUN
american_scientific_journal-2520	141	13	is	be	AUX
american_scientific_journal-2520	141	14	a	a	DET
american_scientific_journal-2520	141	15	ready	ready	ADJ
american_scientific_journal-2520	141	16	-	-	PUNCT
american_scientific_journal-2520	141	17	to	to	PART
american_scientific_journal-2520	141	18	-	-	PUNCT
american_scientific_journal-2520	141	19	use	use	VERB
american_scientific_journal-2520	141	20	reagent	reagent	NOUN
american_scientific_journal-2520	141	21	for	for	ADP
american_scientific_journal-2520	141	22	the	the	DET
american_scientific_journal-2520	141	23	isolation	isolation	NOUN
american_scientific_journal-2520	141	24	of	of	ADP
american_scientific_journal-2520	141	25	total	total	ADJ
american_scientific_journal-2520	141	26	rna	rna	NOUN
american_scientific_journal-2520	141	27	manually	manually	ADV
american_scientific_journal-2520	141	28	from	from	ADP
american_scientific_journal-2520	141	29	cells	cell	NOUN
american_scientific_journal-2520	141	30	and	and	CCONJ
american_scientific_journal-2520	141	31	tissues	tissue	NOUN
american_scientific_journal-2520	142	1	[	[	X
american_scientific_journal-2520	142	2	23	23	NUM
american_scientific_journal-2520	142	3	]	]	PUNCT
american_scientific_journal-2520	142	4	,	,	PUNCT
american_scientific_journal-2520	142	5	the	the	DET
american_scientific_journal-2520	142	6	concentration	concentration	NOUN
american_scientific_journal-2520	142	7	of	of	ADP
american_scientific_journal-2520	142	8	rna	rna	NOUN
american_scientific_journal-2520	142	9	should	should	AUX
american_scientific_journal-2520	142	10	be	be	AUX
american_scientific_journal-2520	142	11	determined	determine	VERB
american_scientific_journal-2520	142	12	by	by	ADP
american_scientific_journal-2520	142	13	measuring	measure	VERB
american_scientific_journal-2520	142	14	the	the	DET
american_scientific_journal-2520	142	15	absorbance	absorbance	NOUN
american_scientific_journal-2520	142	16	at	at	ADP
american_scientific_journal-2520	142	17	260	260	NUM
american_scientific_journal-2520	142	18	nm	nm	NOUN
american_scientific_journal-2520	142	19	(	(	PUNCT
american_scientific_journal-2520	142	20	a260	a260	NUM
american_scientific_journal-2520	142	21	)	)	PUNCT
american_scientific_journal-2520	142	22	in	in	ADP
american_scientific_journal-2520	142	23	a	a	DET
american_scientific_journal-2520	142	24	spectrophotometer	spectrophotometer	NOUN
american_scientific_journal-2520	142	25	.	.	PUNCT
american_scientific_journal-2520	143	1	to	to	PART
american_scientific_journal-2520	143	2	ensure	ensure	VERB
american_scientific_journal-2520	143	3	significance	significance	NOUN
american_scientific_journal-2520	143	4	,	,	PUNCT
american_scientific_journal-2520	143	5	readings	reading	NOUN
american_scientific_journal-2520	143	6	should	should	AUX
american_scientific_journal-2520	143	7	be	be	AUX
american_scientific_journal-2520	143	8	greater	great	ADJ
american_scientific_journal-2520	143	9	than	than	ADP
american_scientific_journal-2520	143	10	0.15	0.15	NUM
american_scientific_journal-2520	143	11	.	.	PUNCT
american_scientific_journal-2520	144	1	an	an	DET
american_scientific_journal-2520	144	2	absorbance	absorbance	NOUN
american_scientific_journal-2520	144	3	of	of	ADP
american_scientific_journal-2520	144	4	1	1	NUM
american_scientific_journal-2520	144	5	unit	unit	NOUN
american_scientific_journal-2520	144	6	at	at	ADP
american_scientific_journal-2520	144	7	260	260	NUM
american_scientific_journal-2520	144	8	nm	nm	NOUN
american_scientific_journal-2520	144	9	corresponds	correspond	NOUN
american_scientific_journal-2520	144	10	to	to	ADP
american_scientific_journal-2520	144	11	40	40	NUM
american_scientific_journal-2520	144	12	μg	μg	PROPN
american_scientific_journal-2520	144	13	of	of	ADP
american_scientific_journal-2520	144	14	rna	rna	PROPN
american_scientific_journal-2520	144	15	per	per	ADP
american_scientific_journal-2520	144	16	ml	ml	PROPN
american_scientific_journal-2520	144	17	[	[	X
american_scientific_journal-2520	144	18	24	24	NUM
american_scientific_journal-2520	144	19	]	]	PUNCT
american_scientific_journal-2520	144	20	.	.	PUNCT
american_scientific_journal-2520	145	1	the	the	DET
american_scientific_journal-2520	145	2	extracted	extract	VERB
american_scientific_journal-2520	145	3	rna	rna	NOUN
american_scientific_journal-2520	145	4	was	be	AUX
american_scientific_journal-2520	145	5	converted	convert	VERB
american_scientific_journal-2520	145	6	to	to	ADP
american_scientific_journal-2520	145	7	cdna	cdna	PROPN
american_scientific_journal-2520	145	8	by	by	ADP
american_scientific_journal-2520	145	9	using	use	VERB
american_scientific_journal-2520	145	10	goscript	goscript	NOUN
american_scientific_journal-2520	145	11	™	™	ADJ
american_scientific_journal-2520	145	12	reverse	reverse	ADJ
american_scientific_journal-2520	145	13	transcription	transcription	NOUN
american_scientific_journal-2520	145	14	system	system	NOUN
american_scientific_journal-2520	145	15	and	and	CCONJ
american_scientific_journal-2520	145	16	random	random	ADJ
american_scientific_journal-2520	145	17	primer	primer	NOUN
american_scientific_journal-2520	145	18	primers	primer	NOUN
american_scientific_journal-2520	145	19	which	which	PRON
american_scientific_journal-2520	145	20	are	be	AUX
american_scientific_journal-2520	145	21	a	a	DET
american_scientific_journal-2520	145	22	mixture	mixture	NOUN
american_scientific_journal-2520	145	23	of	of	ADP
american_scientific_journal-2520	145	24	oligonucleotides	oligonucleotide	NOUN
american_scientific_journal-2520	145	25	representing	represent	VERB
american_scientific_journal-2520	145	26	all	all	DET
american_scientific_journal-2520	145	27	possible	possible	ADJ
american_scientific_journal-2520	145	28	sequence	sequence	NOUN
american_scientific_journal-2520	145	29	for	for	ADP
american_scientific_journal-2520	145	30	that	that	DET
american_scientific_journal-2520	145	31	size	size	NOUN
american_scientific_journal-2520	145	32	.	.	PUNCT
american_scientific_journal-2520	146	1	random	random	ADJ
american_scientific_journal-2520	146	2	primers	primer	NOUN
american_scientific_journal-2520	146	3	can	can	AUX
american_scientific_journal-2520	146	4	be	be	AUX
american_scientific_journal-2520	146	5	used	use	VERB
american_scientific_journal-2520	146	6	to	to	ADP
american_scientific_journal-2520	146	7	cdna	cdna	PROPN
american_scientific_journal-2520	146	8	synthesis	synthesis	NOUN
american_scientific_journal-2520	146	9	.	.	PUNCT
american_scientific_journal-2520	147	1	from	from	ADP
american_scientific_journal-2520	147	2	total	total	ADJ
american_scientific_journal-2520	147	3	rna	rna	PROPN
american_scientific_journal-2520	147	4	or	or	CCONJ
american_scientific_journal-2520	147	5	messenger	messenger	NOUN
american_scientific_journal-2520	147	6	rna	rna	PROPN
american_scientific_journal-2520	147	7	(	(	PUNCT
american_scientific_journal-2520	147	8	mrna	mrna	PROPN
american_scientific_journal-2520	147	9	)	)	PUNCT
american_scientific_journal-2520	148	1	[	[	X
american_scientific_journal-2520	148	2	25	25	NUM
american_scientific_journal-2520	148	3	;	;	PUNCT
american_scientific_journal-2520	148	4	26	26	NUM
american_scientific_journal-2520	148	5	]	]	PUNCT
american_scientific_journal-2520	148	6	.	.	PUNCT
american_scientific_journal-2520	149	1	,	,	PUNCT
american_scientific_journal-2520	149	2	the	the	DET
american_scientific_journal-2520	149	3	rt	rt	PROPN
american_scientific_journal-2520	149	4	pcr	pcr	PROPN
american_scientific_journal-2520	149	5	or	or	CCONJ
american_scientific_journal-2520	149	6	revers	rever	NOUN
american_scientific_journal-2520	149	7	transcription	transcription	NOUN
american_scientific_journal-2520	149	8	pcr	pcr	NOUN
american_scientific_journal-2520	149	9	is	be	AUX
american_scientific_journal-2520	149	10	a	a	DET
american_scientific_journal-2520	149	11	technique	technique	NOUN
american_scientific_journal-2520	149	12	commonly	commonly	ADV
american_scientific_journal-2520	149	13	used	use	VERB
american_scientific_journal-2520	149	14	in	in	ADP
american_scientific_journal-2520	149	15	molecular	molecular	ADJ
american_scientific_journal-2520	149	16	biology	biology	NOUN
american_scientific_journal-2520	149	17	to	to	PART
american_scientific_journal-2520	149	18	detect	detect	VERB
american_scientific_journal-2520	149	19	rna	rna	NOUN
american_scientific_journal-2520	149	20	expression	expression	NOUN
american_scientific_journal-2520	149	21	,	,	PUNCT
american_scientific_journal-2520	149	22	the	the	DET
american_scientific_journal-2520	149	23	rtpcr	rtpcr	NOUN
american_scientific_journal-2520	149	24	is	be	AUX
american_scientific_journal-2520	149	25	abbreviation	abbreviation	NOUN
american_scientific_journal-2520	149	26	of	of	ADP
american_scientific_journal-2520	149	27	real	real	ADJ
american_scientific_journal-2520	149	28	time	time	NOUN
american_scientific_journal-2520	149	29	quantitative	quantitative	ADJ
american_scientific_journal-2520	149	30	reverse	reverse	ADJ
american_scientific_journal-2520	149	31	transcription	transcription	NOUN
american_scientific_journal-2520	149	32	pcr	pcr	NOUN
american_scientific_journal-2520	149	33	)	)	PUNCT
american_scientific_journal-2520	149	34	is	be	AUX
american_scientific_journal-2520	149	35	a	a	DET
american_scientific_journal-2520	149	36	major	major	ADJ
american_scientific_journal-2520	149	37	development	development	NOUN
american_scientific_journal-2520	149	38	of	of	ADP
american_scientific_journal-2520	149	39	pcr	pcr	ADJ
american_scientific_journal-2520	149	40	technology	technology	NOUN
american_scientific_journal-2520	149	41	that	that	PRON
american_scientific_journal-2520	149	42	enables	enable	VERB
american_scientific_journal-2520	149	43	reliable	reliable	ADJ
american_scientific_journal-2520	149	44	detection	detection	NOUN
american_scientific_journal-2520	149	45	and	and	CCONJ
american_scientific_journal-2520	149	46	measurement	measurement	NOUN
american_scientific_journal-2520	149	47	of	of	ADP
american_scientific_journal-2520	149	48	products	product	NOUN
american_scientific_journal-2520	149	49	generated	generate	VERB
american_scientific_journal-2520	149	50	during	during	ADP
american_scientific_journal-2520	149	51	each	each	DET
american_scientific_journal-2520	149	52	cycle	cycle	NOUN
american_scientific_journal-2520	149	53	of	of	ADP
american_scientific_journal-2520	149	54	pcr	pcr	ADJ
american_scientific_journal-2520	149	55	process	process	NOUN
american_scientific_journal-2520	149	56	and	and	CCONJ
american_scientific_journal-2520	149	57	the	the	DET
american_scientific_journal-2520	149	58	real	real	ADJ
american_scientific_journal-2520	149	59	time	time	NOUN
american_scientific_journal-2520	149	60	rt	rt	PROPN
american_scientific_journal-2520	149	61	pcr	pcr	PROPN
american_scientific_journal-2520	149	62	with	with	ADP
american_scientific_journal-2520	149	63	syber	syber	PROPN
american_scientific_journal-2520	149	64	®	®	PROPN
american_scientific_journal-2520	149	65	green	green	ADJ
american_scientific_journal-2520	149	66	detection	detection	NOUN
american_scientific_journal-2520	149	67	produce	produce	VERB
american_scientific_journal-2520	149	68	a	a	DET
american_scientific_journal-2520	149	69	faster	fast	ADJ
american_scientific_journal-2520	149	70	and	and	CCONJ
american_scientific_journal-2520	149	71	dependable	dependable	ADJ
american_scientific_journal-2520	149	72	result	result	NOUN
american_scientific_journal-2520	149	73	[	[	X
american_scientific_journal-2520	149	74	27	27	NUM
american_scientific_journal-2520	149	75	]	]	PUNCT
american_scientific_journal-2520	149	76	,	,	PUNCT
american_scientific_journal-2520	149	77	also	also	ADV
american_scientific_journal-2520	149	78	the	the	DET
american_scientific_journal-2520	149	79	figure	figure	NOUN
american_scientific_journal-2520	149	80	(	(	PUNCT
american_scientific_journal-2520	149	81	8)	8)	NUM
american_scientific_journal-2520	149	82	show	show	VERB
american_scientific_journal-2520	149	83	the	the	DET
american_scientific_journal-2520	149	84	melting	melting	NOUN
american_scientific_journal-2520	149	85	curve	curve	NOUN
american_scientific_journal-2520	149	86	of	of	ADP
american_scientific_journal-2520	149	87	rt	rt	PROPN
american_scientific_journal-2520	149	88	realtime	realtime	NOUN
american_scientific_journal-2520	149	89	pcr	pcr	NOUN
american_scientific_journal-2520	149	90	for	for	ADP
american_scientific_journal-2520	149	91	the	the	DET
american_scientific_journal-2520	149	92	shpth	shpth	ADJ
american_scientific_journal-2520	149	93	gene	gene	NOUN
american_scientific_journal-2520	149	94	,	,	PUNCT
american_scientific_journal-2520	149	95	the	the	DET
american_scientific_journal-2520	149	96	tm	tm	NOUN
american_scientific_journal-2520	149	97	is	be	AUX
american_scientific_journal-2520	149	98	defined	define	VERB
american_scientific_journal-2520	149	99	as	as	ADP
american_scientific_journal-2520	149	100	the	the	DET
american_scientific_journal-2520	149	101	temperature	temperature	NOUN
american_scientific_journal-2520	149	102	at	at	ADP
american_scientific_journal-2520	149	103	which	which	PRON
american_scientific_journal-2520	149	104	50	50	NUM
american_scientific_journal-2520	149	105	%	%	NOUN
american_scientific_journal-2520	149	106	of	of	ADP
american_scientific_journal-2520	149	107	the	the	DET
american_scientific_journal-2520	149	108	helices	helix	NOUN
american_scientific_journal-2520	149	109	are	be	AUX
american_scientific_journal-2520	149	110	dissociated	dissociate	VERB
american_scientific_journal-2520	149	111	.	.	PUNCT
american_scientific_journal-2520	150	1	typically	typically	ADV
american_scientific_journal-2520	150	2	,	,	PUNCT
american_scientific_journal-2520	150	3	the	the	DET
american_scientific_journal-2520	150	4	thermal	thermal	ADJ
american_scientific_journal-2520	150	5	cycler	cycler	NOUN
american_scientific_journal-2520	150	6	being	be	AUX
american_scientific_journal-2520	150	7	used	use	VERB
american_scientific_journal-2520	150	8	to	to	PART
american_scientific_journal-2520	150	9	run	run	VERB
american_scientific_journal-2520	150	10	the	the	DET
american_scientific_journal-2520	150	11	qpcr	qpcr	ADJ
american_scientific_journal-2520	150	12	is	be	AUX
american_scientific_journal-2520	150	13	programmed	program	VERB
american_scientific_journal-2520	150	14	to	to	PART
american_scientific_journal-2520	150	15	produce	produce	VERB
american_scientific_journal-2520	150	16	the	the	DET
american_scientific_journal-2520	150	17	melt	melt	NOUN
american_scientific_journal-2520	150	18	curve	curve	NOUN
american_scientific_journal-2520	150	19	after	after	SCONJ
american_scientific_journal-2520	150	20	the	the	DET
american_scientific_journal-2520	150	21	amplification	amplification	NOUN
american_scientific_journal-2520	150	22	cycles	cycle	NOUN
american_scientific_journal-2520	150	23	are	be	AUX
american_scientific_journal-2520	150	24	completed	complete	VERB
american_scientific_journal-2520	150	25	.	.	PUNCT
american_scientific_journal-2520	151	1	at	at	ADP
american_scientific_journal-2520	151	2	the	the	DET
american_scientific_journal-2520	151	3	end	end	NOUN
american_scientific_journal-2520	151	4	of	of	ADP
american_scientific_journal-2520	151	5	the	the	DET
american_scientific_journal-2520	151	6	qpcr	qpcr	ADJ
american_scientific_journal-2520	151	7	run	run	NOUN
american_scientific_journal-2520	151	8	,	,	PUNCT
american_scientific_journal-2520	151	9	the	the	DET
american_scientific_journal-2520	151	10	thermal	thermal	ADJ
american_scientific_journal-2520	151	11	cycler	cycler	NOUN
american_scientific_journal-2520	151	12	starts	start	VERB
american_scientific_journal-2520	151	13	at	at	ADP
american_scientific_journal-2520	151	14	a	a	DET
american_scientific_journal-2520	151	15	preset	preset	ADJ
american_scientific_journal-2520	151	16	temperature	temperature	NOUN
american_scientific_journal-2520	151	17	(	(	PUNCT
american_scientific_journal-2520	151	18	usually	usually	ADV
american_scientific_journal-2520	151	19	above	above	ADP
american_scientific_journal-2520	151	20	the	the	DET
american_scientific_journal-2520	151	21	primer	primer	NOUN
american_scientific_journal-2520	151	22	tm	tm	NOUN
american_scientific_journal-2520	151	23	;	;	PUNCT
american_scientific_journal-2520	151	24	e.g.	e.g.	ADV
american_scientific_journal-2520	151	25	,	,	PUNCT
american_scientific_journal-2520	151	26	60	60	NUM
american_scientific_journal-2520	151	27	°	°	NUM
american_scientific_journal-2520	151	28	c	c	NOUN
american_scientific_journal-2520	151	29	)	)	PUNCT
american_scientific_journal-2520	151	30	and	and	CCONJ
american_scientific_journal-2520	151	31	measures	measure	VERB
american_scientific_journal-2520	151	32	the	the	DET
american_scientific_journal-2520	151	33	amount	amount	NOUN
american_scientific_journal-2520	151	34	of	of	ADP
american_scientific_journal-2520	151	35	fluorescence	fluorescence	NOUN
american_scientific_journal-2520	151	36	.	.	PUNCT
american_scientific_journal-2520	152	1	the	the	DET
american_scientific_journal-2520	152	2	temperature	temperature	NOUN
american_scientific_journal-2520	152	3	of	of	ADP
american_scientific_journal-2520	152	4	the	the	DET
american_scientific_journal-2520	152	5	sample	sample	NOUN
american_scientific_journal-2520	152	6	is	be	AUX
american_scientific_journal-2520	152	7	then	then	ADV
american_scientific_journal-2520	152	8	https://en.wikipedia.org/wiki/nucleotide	https://en.wikipedia.org/wiki/nucleotide	PROPN
american_scientific_journal-2520	152	9	https://en.wikipedia.org/wiki/messenger_rna	https://en.wikipedia.org/wiki/messenger_rna	PROPN
american_scientific_journal-2520	152	10	https://en.wikipedia.org/wiki/translation_(biology	https://en.wikipedia.org/wiki/translation_(biology	NOUN
american_scientific_journal-2520	152	11	)	)	PUNCT
american_scientific_journal-2520	153	1	https://en.wikipedia.org/wiki/rna	https://en.wikipedia.org/wiki/rna	PROPN
american_scientific_journal-2520	153	2	https://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/techpcr.shtml	https://www.ncbi.nlm.nih.gov/projects/genome/probe/doc/techpcr.shtml	PROPN
american_scientific_journal-2520	153	3	american	american	PROPN
american_scientific_journal-2520	153	4	scientific	scientific	ADJ
american_scientific_journal-2520	153	5	research	research	PROPN
american_scientific_journal-2520	153	6	journal	journal	NOUN
american_scientific_journal-2520	153	7	for	for	ADP
american_scientific_journal-2520	153	8	engineering	engineering	NOUN
american_scientific_journal-2520	153	9	,	,	PUNCT
american_scientific_journal-2520	153	10	technology	technology	NOUN
american_scientific_journal-2520	153	11	,	,	PUNCT
american_scientific_journal-2520	153	12	and	and	CCONJ
american_scientific_journal-2520	153	13	sciences	science	NOUN
american_scientific_journal-2520	153	14	(	(	PUNCT
american_scientific_journal-2520	153	15	asrjets	asrjet	NOUN
american_scientific_journal-2520	153	16	)	)	PUNCT
american_scientific_journal-2520	153	17	(	(	PUNCT
american_scientific_journal-2520	153	18	2017	2017	NUM
american_scientific_journal-2520	153	19	)	)	PUNCT
american_scientific_journal-2520	153	20	volume	volume	NOUN
american_scientific_journal-2520	153	21	27	27	NUM
american_scientific_journal-2520	153	22	,	,	PUNCT
american_scientific_journal-2520	153	23	no	no	DET
american_scientific_journal-2520	153	24	1	1	NUM
american_scientific_journal-2520	153	25	,	,	PUNCT
american_scientific_journal-2520	153	26	pp	pp	ADV
american_scientific_journal-2520	153	27	12	12	NUM
american_scientific_journal-2520	153	28	-	-	SYM
american_scientific_journal-2520	153	29	24	24	NUM
american_scientific_journal-2520	153	30	22	22	NUM
american_scientific_journal-2520	153	31	increased	increase	VERB
american_scientific_journal-2520	153	32	incrementally	incrementally	ADV
american_scientific_journal-2520	153	33	as	as	SCONJ
american_scientific_journal-2520	153	34	the	the	DET
american_scientific_journal-2520	153	35	instrument	instrument	NOUN
american_scientific_journal-2520	153	36	continues	continue	VERB
american_scientific_journal-2520	153	37	to	to	PART
american_scientific_journal-2520	153	38	measure	measure	VERB
american_scientific_journal-2520	153	39	fluorescence	fluorescence	NOUN
american_scientific_journal-2520	153	40	.	.	PUNCT
american_scientific_journal-2520	154	1	as	as	ADP
american_scientific_journal-2520	154	2	the	the	DET
american_scientific_journal-2520	154	3	temperature	temperature	NOUN
american_scientific_journal-2520	154	4	increases	increase	NOUN
american_scientific_journal-2520	154	5	,	,	PUNCT
american_scientific_journal-2520	154	6	dsdna	dsdna	NOUN
american_scientific_journal-2520	154	7	denatures	denature	NOUN
american_scientific_journal-2520	154	8	becoming	become	VERB
american_scientific_journal-2520	154	9	single	single	ADJ
american_scientific_journal-2520	154	10	-	-	PUNCT
american_scientific_journal-2520	154	11	stranded	strand	VERB
american_scientific_journal-2520	154	12	,	,	PUNCT
american_scientific_journal-2520	154	13	and	and	CCONJ
american_scientific_journal-2520	154	14	the	the	DET
american_scientific_journal-2520	154	15	dye	dye	NOUN
american_scientific_journal-2520	154	16	dissociates	dissociate	NOUN
american_scientific_journal-2520	154	17	,	,	PUNCT
american_scientific_journal-2520	154	18	resulting	result	VERB
american_scientific_journal-2520	154	19	in	in	ADP
american_scientific_journal-2520	154	20	decreasing	decrease	VERB
american_scientific_journal-2520	154	21	fluorescence	fluorescence	NOUN
american_scientific_journal-2520	154	22	as	as	SCONJ
american_scientific_journal-2520	154	23	shown	show	VERB
american_scientific_journal-2520	154	24	in	in	ADP
american_scientific_journal-2520	154	25	figure	figure	NOUN
american_scientific_journal-2520	154	26	(	(	PUNCT
american_scientific_journal-2520	154	27	8)	8)	NUM
american_scientific_journal-2520	154	28	[	[	X
american_scientific_journal-2520	154	29	28	28	NUM
american_scientific_journal-2520	154	30	]	]	PUNCT
american_scientific_journal-2520	154	31	.	.	PUNCT
american_scientific_journal-2520	155	1	and	and	CCONJ
american_scientific_journal-2520	155	2	the	the	DET
american_scientific_journal-2520	155	3	figure	figure	NOUN
american_scientific_journal-2520	155	4	(	(	PUNCT
american_scientific_journal-2520	155	5	7	7	X
american_scientific_journal-2520	155	6	)	)	PUNCT
american_scientific_journal-2520	155	7	show	show	VERB
american_scientific_journal-2520	155	8	a	a	DET
american_scientific_journal-2520	155	9	high	high	ADJ
american_scientific_journal-2520	155	10	ct	ct	NOUN
american_scientific_journal-2520	155	11	of	of	ADP
american_scientific_journal-2520	155	12	qrt	qrt	ADJ
american_scientific_journal-2520	155	13	real	real	ADJ
american_scientific_journal-2520	155	14	-	-	PUNCT
american_scientific_journal-2520	155	15	time	time	NOUN
american_scientific_journal-2520	155	16	pcr	pcr	NOUN
american_scientific_journal-2520	155	17	of	of	ADP
american_scientific_journal-2520	155	18	the	the	DET
american_scientific_journal-2520	155	19	target	target	NOUN
american_scientific_journal-2520	155	20	gene	gene	NOUN
american_scientific_journal-2520	155	21	,	,	PUNCT
american_scientific_journal-2520	155	22	ct	ct	PRON
american_scientific_journal-2520	155	23	mean	mean	VERB
american_scientific_journal-2520	155	24	the	the	DET
american_scientific_journal-2520	155	25	spot	spot	NOUN
american_scientific_journal-2520	155	26	where	where	SCONJ
american_scientific_journal-2520	155	27	the	the	DET
american_scientific_journal-2520	155	28	reaction	reaction	NOUN
american_scientific_journal-2520	155	29	curve	curve	NOUN
american_scientific_journal-2520	155	30	intersects	intersect	NOUN
american_scientific_journal-2520	155	31	with	with	ADP
american_scientific_journal-2520	155	32	threshold	threshold	NOUN
american_scientific_journal-2520	155	33	line	line	NOUN
american_scientific_journal-2520	155	34	(	(	PUNCT
american_scientific_journal-2520	155	35	a	a	DET
american_scientific_journal-2520	155	36	line	line	NOUN
american_scientific_journal-2520	155	37	in	in	ADP
american_scientific_journal-2520	155	38	the	the	DET
american_scientific_journal-2520	155	39	graph	graph	NOUN
american_scientific_journal-2520	155	40	that	that	PRON
american_scientific_journal-2520	155	41	represents	represent	VERB
american_scientific_journal-2520	155	42	a	a	DET
american_scientific_journal-2520	155	43	level	level	NOUN
american_scientific_journal-2520	155	44	above	above	ADP
american_scientific_journal-2520	155	45	background	background	NOUN
american_scientific_journal-2520	155	46	fluorescence	fluorescence	NOUN
american_scientific_journal-2520	155	47	)	)	PUNCT
american_scientific_journal-2520	155	48	is	be	AUX
american_scientific_journal-2520	155	49	the	the	DET
american_scientific_journal-2520	155	50	ct	ct	PROPN
american_scientific_journal-2520	155	51	,	,	PUNCT
american_scientific_journal-2520	155	52	or	or	CCONJ
american_scientific_journal-2520	155	53	“	"	PUNCT
american_scientific_journal-2520	155	54	threshold	threshold	NOUN
american_scientific_journal-2520	155	55	cycle	cycle	NOUN
american_scientific_journal-2520	155	56	.	.	PUNCT
american_scientific_journal-2520	155	57	”	"	PUNCT
american_scientific_journal-2520	156	1	this	this	DET
american_scientific_journal-2520	156	2	spot	spot	NOUN
american_scientific_journal-2520	156	3	shows	show	VERB
american_scientific_journal-2520	156	4	the	the	DET
american_scientific_journal-2520	156	5	number	number	NOUN
american_scientific_journal-2520	156	6	of	of	ADP
american_scientific_journal-2520	156	7	cycles	cycle	NOUN
american_scientific_journal-2520	156	8	it	it	PRON
american_scientific_journal-2520	156	9	took	take	VERB
american_scientific_journal-2520	156	10	to	to	PART
american_scientific_journal-2520	156	11	detect	detect	VERB
american_scientific_journal-2520	156	12	a	a	DET
american_scientific_journal-2520	156	13	real	real	ADJ
american_scientific_journal-2520	156	14	signal	signal	NOUN
american_scientific_journal-2520	156	15	from	from	ADP
american_scientific_journal-2520	156	16	the	the	DET
american_scientific_journal-2520	156	17	samples	sample	NOUN
american_scientific_journal-2520	156	18	.	.	PUNCT
american_scientific_journal-2520	157	1	generally	generally	ADV
american_scientific_journal-2520	157	2	for	for	ADP
american_scientific_journal-2520	157	3	high	high	ADJ
american_scientific_journal-2520	157	4	gene	gene	NOUN
american_scientific_journal-2520	157	5	expression	expression	NOUN
american_scientific_journal-2520	157	6	the	the	DET
american_scientific_journal-2520	157	7	ct	ct	PROPN
american_scientific_journal-2520	157	8	must	must	AUX
american_scientific_journal-2520	157	9	be	be	AUX
american_scientific_journal-2520	157	10	below	below	ADP
american_scientific_journal-2520	157	11	30	30	NUM
american_scientific_journal-2520	157	12	cycles	cycle	NOUN
american_scientific_journal-2520	157	13	,	,	PUNCT
american_scientific_journal-2520	157	14	and	and	CCONJ
american_scientific_journal-2520	157	15	its	its	PRON
american_scientific_journal-2520	157	16	(	(	PUNCT
american_scientific_journal-2520	157	17	23.46	23.46	NUM
american_scientific_journal-2520	157	18	)	)	PUNCT
american_scientific_journal-2520	157	19	for	for	ADP
american_scientific_journal-2520	157	20	the	the	DET
american_scientific_journal-2520	157	21	target	target	NOUN
american_scientific_journal-2520	157	22	gene	gene	NOUN
american_scientific_journal-2520	157	23	which	which	PRON
american_scientific_journal-2520	157	24	indicate	indicate	VERB
american_scientific_journal-2520	157	25	a	a	DET
american_scientific_journal-2520	157	26	high	high	ADJ
american_scientific_journal-2520	157	27	gene	gene	NOUN
american_scientific_journal-2520	157	28	expression	expression	NOUN
american_scientific_journal-2520	157	29	[	[	X
american_scientific_journal-2520	157	30	29	29	NUM
american_scientific_journal-2520	157	31	]	]	PUNCT
american_scientific_journal-2520	157	32	.	.	PUNCT
american_scientific_journal-2520	158	1	5	5	X
american_scientific_journal-2520	158	2	.	.	X
american_scientific_journal-2520	158	3	conclusions	conclusion	NOUN
american_scientific_journal-2520	158	4	the	the	DET
american_scientific_journal-2520	158	5	cloning	cloning	NOUN
american_scientific_journal-2520	158	6	and	and	CCONJ
american_scientific_journal-2520	158	7	expression	expression	NOUN
american_scientific_journal-2520	158	8	of	of	ADP
american_scientific_journal-2520	158	9	normal	normal	ADJ
american_scientific_journal-2520	158	10	human	human	ADJ
american_scientific_journal-2520	158	11	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	158	12	hormone	hormone	NOUN
american_scientific_journal-2520	158	13	gene	gene	NOUN
american_scientific_journal-2520	158	14	in	in	ADP
american_scientific_journal-2520	158	15	bacterial	bacterial	ADJ
american_scientific_journal-2520	158	16	strain	strain	NOUN
american_scientific_journal-2520	158	17	led	lead	VERB
american_scientific_journal-2520	158	18	to	to	ADP
american_scientific_journal-2520	158	19	production	production	NOUN
american_scientific_journal-2520	158	20	of	of	ADP
american_scientific_journal-2520	158	21	biologically	biologically	ADV
american_scientific_journal-2520	158	22	inactive	inactive	ADJ
american_scientific_journal-2520	158	23	hormone	hormone	NOUN
american_scientific_journal-2520	158	24	because	because	SCONJ
american_scientific_journal-2520	158	25	the	the	DET
american_scientific_journal-2520	158	26	bacteria	bacteria	NOUN
american_scientific_journal-2520	158	27	lack	lack	AUX
american_scientific_journal-2520	158	28	post	post	VERB
american_scientific_journal-2520	158	29	translational	translational	ADJ
american_scientific_journal-2520	158	30	modification	modification	NOUN
american_scientific_journal-2520	158	31	that	that	PRON
american_scientific_journal-2520	158	32	present	present	VERB
american_scientific_journal-2520	158	33	in	in	ADP
american_scientific_journal-2520	158	34	higher	high	ADJ
american_scientific_journal-2520	158	35	cell	cell	NOUN
american_scientific_journal-2520	158	36	organism	organism	NOUN
american_scientific_journal-2520	158	37	.	.	PUNCT
american_scientific_journal-2520	159	1	therefore	therefore	ADV
american_scientific_journal-2520	159	2	,	,	PUNCT
american_scientific_journal-2520	159	3	in	in	ADP
american_scientific_journal-2520	159	4	the	the	DET
american_scientific_journal-2520	159	5	current	current	ADJ
american_scientific_journal-2520	159	6	study	study	NOUN
american_scientific_journal-2520	159	7	,	,	PUNCT
american_scientific_journal-2520	159	8	a	a	DET
american_scientific_journal-2520	159	9	synthetic	synthetic	ADJ
american_scientific_journal-2520	159	10	recombinant	recombinant	ADJ
american_scientific_journal-2520	159	11	human	human	ADJ
american_scientific_journal-2520	159	12	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	159	13	hormone	hormone	NOUN
american_scientific_journal-2520	159	14	gene	gene	NOUN
american_scientific_journal-2520	159	15	that	that	PRON
american_scientific_journal-2520	159	16	’s	’	VERB
american_scientific_journal-2520	159	17	code	code	NOUN
american_scientific_journal-2520	159	18	only	only	ADV
american_scientific_journal-2520	159	19	for	for	ADP
american_scientific_journal-2520	159	20	the	the	DET
american_scientific_journal-2520	159	21	active	active	ADJ
american_scientific_journal-2520	159	22	hormone	hormone	NOUN
american_scientific_journal-2520	159	23	form	form	NOUN
american_scientific_journal-2520	159	24	was	be	AUX
american_scientific_journal-2520	159	25	constructed	construct	VERB
american_scientific_journal-2520	159	26	by	by	ADP
american_scientific_journal-2520	159	27	five	five	NUM
american_scientific_journal-2520	159	28	long	long	ADJ
american_scientific_journal-2520	159	29	primer	primer	NOUN
american_scientific_journal-2520	159	30	using	use	VERB
american_scientific_journal-2520	159	31	soe	soe	PROPN
american_scientific_journal-2520	159	32	pcr	pcr	NOUN
american_scientific_journal-2520	159	33	,	,	PUNCT
american_scientific_journal-2520	159	34	and	and	CCONJ
american_scientific_journal-2520	159	35	then	then	ADV
american_scientific_journal-2520	159	36	expressed	express	VERB
american_scientific_journal-2520	159	37	in	in	ADP
american_scientific_journal-2520	159	38	e.	e.	PROPN
american_scientific_journal-2520	159	39	coli	coli	PROPN
american_scientific_journal-2520	159	40	strain	strain	NOUN
american_scientific_journal-2520	159	41	.	.	PUNCT
american_scientific_journal-2520	160	1	6	6	X
american_scientific_journal-2520	160	2	.	.	X
american_scientific_journal-2520	160	3	recommendation	recommendation	NOUN
american_scientific_journal-2520	160	4	we	we	PRON
american_scientific_journal-2520	160	5	recommended	recommend	VERB
american_scientific_journal-2520	160	6	using	use	VERB
american_scientific_journal-2520	160	7	the	the	DET
american_scientific_journal-2520	160	8	cloning	cloning	NOUN
american_scientific_journal-2520	160	9	isolate	isolate	NOUN
american_scientific_journal-2520	160	10	with	with	ADP
american_scientific_journal-2520	160	11	shpth	shpth	ADJ
american_scientific_journal-2520	160	12	gene	gene	NOUN
american_scientific_journal-2520	160	13	for	for	ADP
american_scientific_journal-2520	160	14	production	production	NOUN
american_scientific_journal-2520	160	15	of	of	ADP
american_scientific_journal-2520	160	16	the	the	DET
american_scientific_journal-2520	160	17	human	human	ADJ
american_scientific_journal-2520	160	18	parathyroid	parathyroid	ADJ
american_scientific_journal-2520	160	19	hormone	hormone	NOUN
american_scientific_journal-2520	160	20	to	to	PART
american_scientific_journal-2520	160	21	estimate	estimate	VERB
american_scientific_journal-2520	160	22	the	the	DET
american_scientific_journal-2520	160	23	chemical	chemical	ADJ
american_scientific_journal-2520	160	24	and	and	CCONJ
american_scientific_journal-2520	160	25	biological	biological	ADJ
american_scientific_journal-2520	160	26	features	feature	NOUN
american_scientific_journal-2520	160	27	for	for	ADP
american_scientific_journal-2520	160	28	the	the	DET
american_scientific_journal-2520	160	29	produced	produce	VERB
american_scientific_journal-2520	160	30	hormone	hormone	NOUN
american_scientific_journal-2520	160	31	.	.	PUNCT
american_scientific_journal-2520	161	1	supplementary	supplementary	ADJ
american_scientific_journal-2520	161	2	materials	material	NOUN
american_scientific_journal-2520	161	3	:	:	PUNCT
american_scientific_journal-2520	161	4	the	the	DET
american_scientific_journal-2520	161	5	following	follow	VERB
american_scientific_journal-2520	161	6	are	be	AUX
american_scientific_journal-2520	161	7	available	available	ADJ
american_scientific_journal-2520	161	8	online	online	ADV
american_scientific_journal-2520	161	9	at	at	ADP
american_scientific_journal-2520	161	10	https://www	https://www	PROPN
american_scientific_journal-2520	161	11	.	.	PUNCT
american_scientific_journal-2520	162	1	thermofisher	thermofisher	PROPN
american_scientific_journal-2520	162	2	.	.	PUNCT
american_scientific_journal-2520	162	3	com	com	NOUN
american_scientific_journal-2520	162	4	/	/	SYM
american_scientific_journal-2520	162	5	iq	iq	NOUN
american_scientific_journal-2520	162	6	/	/	SYM
american_scientific_journal-2520	162	7	en	en	NOUN
american_scientific_journal-2520	162	8	/	/	SYM
american_scientific_journal-2520	162	9	home	home	NOUN
american_scientific_journal-2520	162	10	/	/	SYM
american_scientific_journal-2520	162	11	life	life	NOUN
american_scientific_journal-2520	162	12	-	-	PUNCT
american_scientific_journal-2520	162	13	science	science	NOUN
american_scientific_journal-2520	162	14	/	/	SYM
american_scientific_journal-2520	162	15	pcr	pcr	ADJ
american_scientific_journal-2520	162	16	/	/	SYM
american_scientific_journal-2520	162	17	real	real	ADJ
american_scientific_journal-2520	162	18	-	-	PUNCT
american_scientific_journal-2520	162	19	timepcr	timepcr	NOUN
american_scientific_journal-2520	162	20	/	/	SYM
american_scientific_journal-2520	162	21	real	real	ADJ
american_scientific_journal-2520	162	22	-	-	PUNCT
american_scientific_journal-2520	162	23	time	time	NOUN
american_scientific_journal-2520	162	24	-	-	PUNCT
american_scientific_journal-2520	162	25	pcr	pcr	ADJ
american_scientific_journal-2520	162	26	-	-	PUNCT
american_scientific_journal-2520	162	27	applications	application	NOUN
american_scientific_journal-2520	162	28	/	/	SYM
american_scientific_journal-2520	162	29	gene	gene	NOUN
american_scientific_journal-2520	162	30	-	-	PUNCT
american_scientific_journal-2520	162	31	expression	expression	NOUN
american_scientific_journal-2520	162	32	-	-	PUNCT
american_scientific_journal-2520	162	33	using	use	VERB
american_scientific_journal-2520	162	34	-	-	PUNCT
american_scientific_journal-2520	162	35	real	real	ADJ
american_scientific_journal-2520	162	36	-	-	PUNCT
american_scientific_journal-2520	162	37	time	time	NOUN
american_scientific_journal-2520	162	38	-	-	PUNCT
american_scientific_journal-2520	162	39	pcr.html	pcr.html	NOUN
american_scientific_journal-2520	162	40	.	.	PUNCT
american_scientific_journal-2520	163	1	gene	gene	NOUN
american_scientific_journal-2520	163	2	expression	expression	NOUN
american_scientific_journal-2520	163	3	analysis	analysis	NOUN
american_scientific_journal-2520	163	4	using	use	VERB
american_scientific_journal-2520	163	5	realtime	realtime	NOUN
american_scientific_journal-2520	163	6	pcr	pcr	NOUN
american_scientific_journal-2520	163	7	and	and	CCONJ
american_scientific_journal-2520	163	8	http://	http://	PROPN
american_scientific_journal-2520	163	9	classroom.synonym.com/advantages-using-sticky-end-enzymes-17470.html	classroom.synonym.com/advantages-using-sticky-end-enzymes-17470.html	PROPN
american_scientific_journal-2520	163	10	for	for	ADP
american_scientific_journal-2520	163	11	the	the	DET
american_scientific_journal-2520	163	12	advantages	advantage	NOUN
american_scientific_journal-2520	163	13	of	of	ADP
american_scientific_journal-2520	163	14	using	use	VERB
american_scientific_journal-2520	163	15	sticky	sticky	ADJ
american_scientific_journal-2520	163	16	end	end	NOUN
american_scientific_journal-2520	163	17	enzyme	enzyme	NOUN
american_scientific_journal-2520	163	18	.	.	PUNCT
american_scientific_journal-2520	164	1	acknowledgments	acknowledgment	NOUN
american_scientific_journal-2520	164	2	the	the	DET
american_scientific_journal-2520	164	3	authors	author	NOUN
american_scientific_journal-2520	164	4	are	be	AUX
american_scientific_journal-2520	164	5	grateful	grateful	ADJ
american_scientific_journal-2520	164	6	to	to	ADP
american_scientific_journal-2520	164	7	the	the	DET
american_scientific_journal-2520	164	8	laboratory	laboratory	NOUN
american_scientific_journal-2520	164	9	of	of	ADP
american_scientific_journal-2520	164	10	cell	cell	NOUN
american_scientific_journal-2520	164	11	and	and	CCONJ
american_scientific_journal-2520	164	12	biotechnology	biotechnology	NOUN
american_scientific_journal-2520	164	13	researches	research	VERB
american_scientific_journal-2520	164	14	unit	unit	NOUN
american_scientific_journal-2520	164	15	.	.	PUNCT
american_scientific_journal-2520	165	1	author	author	NOUN
american_scientific_journal-2520	165	2	contributions	contributions	PROPN
american_scientific_journal-2520	165	3	dr	dr	PROPN
american_scientific_journal-2520	165	4	.	.	PROPN
american_scientific_journal-2520	165	5	adnan	adnan	PROPN
american_scientific_journal-2520	166	1	al	al	PROPN
american_scientific_journal-2520	166	2	-	-	PUNCT
american_scientific_journal-2520	166	3	badran	badran	PROPN
american_scientific_journal-2520	166	4	contributed	contribute	VERB
american_scientific_journal-2520	166	5	to	to	ADP
american_scientific_journal-2520	166	6	the	the	DET
american_scientific_journal-2520	166	7	conception	conception	NOUN
american_scientific_journal-2520	166	8	and	and	CCONJ
american_scientific_journal-2520	166	9	design	design	NOUN
american_scientific_journal-2520	166	10	of	of	ADP
american_scientific_journal-2520	166	11	the	the	DET
american_scientific_journal-2520	166	12	study	study	NOUN
american_scientific_journal-2520	166	13	,	,	PUNCT
american_scientific_journal-2520	166	14	rafeef	rafeef	ADJ
american_scientific_journal-2520	166	15	abdul	abdul	PROPN
american_scientific_journal-2520	166	16	-	-	PUNCT
american_scientific_journal-2520	166	17	jabbar	jabbar	PROPN
american_scientific_journal-2520	166	18	contributed	contribute	VERB
american_scientific_journal-2520	166	19	to	to	ADP
american_scientific_journal-2520	166	20	the	the	DET
american_scientific_journal-2520	166	21	conception	conception	NOUN
american_scientific_journal-2520	166	22	and	and	CCONJ
american_scientific_journal-2520	166	23	design	design	NOUN
american_scientific_journal-2520	166	24	of	of	ADP
american_scientific_journal-2520	166	25	the	the	DET
american_scientific_journal-2520	166	26	study	study	NOUN
american_scientific_journal-2520	166	27	,	,	PUNCT
american_scientific_journal-2520	166	28	performed	perform	VERB
american_scientific_journal-2520	166	29	experimental	experimental	ADJ
american_scientific_journal-2520	166	30	work	work	NOUN
american_scientific_journal-2520	166	31	and	and	CCONJ
american_scientific_journal-2520	166	32	participated	participate	VERB
american_scientific_journal-2520	166	33	in	in	ADP
american_scientific_journal-2520	166	34	data	datum	NOUN
american_scientific_journal-2520	166	35	analysis	analysis	NOUN
american_scientific_journal-2520	166	36	.	.	PUNCT
american_scientific_journal-2520	167	1	both	both	DET
american_scientific_journal-2520	167	2	authors	author	NOUN
american_scientific_journal-2520	167	3	read	read	VERB
american_scientific_journal-2520	167	4	and	and	CCONJ
american_scientific_journal-2520	167	5	approved	approve	VERB
american_scientific_journal-2520	167	6	the	the	DET
american_scientific_journal-2520	167	7	final	final	ADJ
american_scientific_journal-2520	167	8	manuscript	manuscript	NOUN
american_scientific_journal-2520	167	9	.	.	PUNCT
american_scientific_journal-2520	168	1	conflicts	conflict	NOUN
american_scientific_journal-2520	168	2	of	of	ADP
american_scientific_journal-2520	168	3	interest	interest	NOUN
american_scientific_journal-2520	168	4	the	the	DET
american_scientific_journal-2520	168	5	authors	author	NOUN
american_scientific_journal-2520	168	6	declare	declare	VERB
american_scientific_journal-2520	168	7	no	no	DET
american_scientific_journal-2520	168	8	conflict	conflict	NOUN
american_scientific_journal-2520	168	9	of	of	ADP
american_scientific_journal-2520	168	10	interest	interest	NOUN
american_scientific_journal-2520	168	11	.	.	PUNCT
american_scientific_journal-2520	169	1	american	american	ADJ
american_scientific_journal-2520	169	2	scientific	scientific	ADJ
american_scientific_journal-2520	169	3	research	research	PROPN
american_scientific_journal-2520	169	4	journal	journal	NOUN
american_scientific_journal-2520	169	5	for	for	ADP
american_scientific_journal-2520	169	6	engineering	engineering	NOUN
american_scientific_journal-2520	169	7	,	,	PUNCT
american_scientific_journal-2520	169	8	technology	technology	NOUN
american_scientific_journal-2520	169	9	,	,	PUNCT
american_scientific_journal-2520	169	10	and	and	CCONJ
american_scientific_journal-2520	169	11	sciences	science	NOUN
american_scientific_journal-2520	169	12	(	(	PUNCT
american_scientific_journal-2520	169	13	asrjets	asrjet	NOUN
american_scientific_journal-2520	169	14	)	)	PUNCT
american_scientific_journal-2520	169	15	(	(	PUNCT
american_scientific_journal-2520	169	16	2017	2017	NUM
american_scientific_journal-2520	169	17	)	)	PUNCT
american_scientific_journal-2520	169	18	volume	volume	NOUN
american_scientific_journal-2520	169	19	27	27	NUM
american_scientific_journal-2520	169	20	,	,	PUNCT
american_scientific_journal-2520	169	21	no	no	DET
american_scientific_journal-2520	169	22	1	1	NUM
american_scientific_journal-2520	169	23	,	,	PUNCT
american_scientific_journal-2520	169	24	pp	pp	ADV
american_scientific_journal-2520	169	25	12	12	NUM
american_scientific_journal-2520	169	26	-	-	SYM
american_scientific_journal-2520	169	27	24	24	NUM
american_scientific_journal-2520	169	28	23	23	NUM
american_scientific_journal-2520	169	29	reference	reference	NOUN
american_scientific_journal-2520	169	30	[	[	X
american_scientific_journal-2520	169	31	1	1	NUM
american_scientific_journal-2520	169	32	]	]	PUNCT
american_scientific_journal-2520	169	33	bilezikian	bilezikian	NOUN
american_scientific_journal-2520	169	34	,	,	PUNCT
american_scientific_journal-2520	169	35	j.	j.	PROPN
american_scientific_journal-2520	169	36	p	p	PROPN
american_scientific_journal-2520	169	37	,	,	PUNCT
american_scientific_journal-2520	169	38	marcus	marcus	PROPN
american_scientific_journal-2520	169	39	,	,	PUNCT
american_scientific_journal-2520	169	40	r.	r.	PROPN
american_scientific_journal-2520	169	41	,	,	PUNCT
american_scientific_journal-2520	169	42	levine	levine	PROPN
american_scientific_journal-2520	169	43	,	,	PUNCT
american_scientific_journal-2520	169	44	m.	m.	NOUN
american_scientific_journal-2520	169	45	a.	a.	PROPN
american_scientific_journal-2520	169	46	,	,	PUNCT
american_scientific_journal-2520	169	47	marcocci	marcocci	PROPN
american_scientific_journal-2520	169	48	,	,	PUNCT
american_scientific_journal-2520	169	49	c	c	PROPN
american_scientific_journal-2520	169	50	,	,	PUNCT
american_scientific_journal-2520	169	51	silverberg	silverberg	PROPN
american_scientific_journal-2520	169	52	,	,	PUNCT
american_scientific_journal-2520	169	53	s.j	s.j	PROPN
american_scientific_journal-2520	169	54	,	,	PUNCT
american_scientific_journal-2520	169	55	potts	potts	PROPN
american_scientific_journal-2520	169	56	,	,	PUNCT
american_scientific_journal-2520	169	57	j.	j.	PROPN
american_scientific_journal-2520	169	58	t.	t.	PROPN
american_scientific_journal-2520	169	59	the	the	DET
american_scientific_journal-2520	169	60	parathyroids	parathyroid	NOUN
american_scientific_journal-2520	169	61	:	:	PUNCT
american_scientific_journal-2520	169	62	basic	basic	ADJ
american_scientific_journal-2520	169	63	and	and	CCONJ
american_scientific_journal-2520	169	64	clinical	clinical	ADJ
american_scientific_journal-2520	169	65	concepts	concept	NOUN
american_scientific_journal-2520	169	66	.	.	PUNCT
american_scientific_journal-2520	170	1	academic	academic	PROPN
american_scientific_journal-2520	170	2	press.3rd	press.3rd	PROPN
american_scientific_journal-2520	170	3	edition	edition	PROPN
american_scientific_journal-2520	170	4	,	,	PUNCT
american_scientific_journal-2520	170	5	print	print	NOUN
american_scientific_journal-2520	170	6	book	book	NOUN
american_scientific_journal-2520	170	7	isbn	isbn	NOUN
american_scientific_journal-2520	170	8	:	:	PUNCT
american_scientific_journal-2520	170	9	9780123971661	9780123971661	NUM
american_scientific_journal-2520	170	10	.	.	PUNCT
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american_scientific_journal-2520	171	2	isbn	isbn	NOUN
american_scientific_journal-2520	171	3	:	:	PUNCT
american_scientific_journal-2520	171	4	9780123977908	9780123977908	NUM
american_scientific_journal-2520	171	5	,	,	PUNCT
american_scientific_journal-2520	171	6	2014	2014	NUM
american_scientific_journal-2520	171	7	.	.	PUNCT
american_scientific_journal-2520	172	1	pages	page	NOUN
american_scientific_journal-2520	172	2	:	:	PUNCT
american_scientific_journal-2520	172	3	946	946	NUM
american_scientific_journal-2520	172	4	.	.	PUNCT
american_scientific_journal-2520	173	1	[	[	X
american_scientific_journal-2520	173	2	2	2	NUM
american_scientific_journal-2520	173	3	]	]	X
american_scientific_journal-2520	173	4	goswami	goswami	NOUN
american_scientific_journal-2520	173	5	,	,	PUNCT
american_scientific_journal-2520	173	6	r.	r.	PROPN
american_scientific_journal-2520	173	7	,	,	PUNCT
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american_scientific_journal-2520	173	9	,	,	PUNCT
american_scientific_journal-2520	173	10	t.	t.	PROPN
american_scientific_journal-2520	173	11	,	,	PUNCT
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american_scientific_journal-2520	173	13	,	,	PUNCT
american_scientific_journal-2520	173	14	n.	n.	NOUN
american_scientific_journal-2520	173	15	,	,	PUNCT
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american_scientific_journal-2520	173	17	,	,	PUNCT
american_scientific_journal-2520	173	18	r.	r.	PROPN
american_scientific_journal-2520	173	19	,	,	PUNCT
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american_scientific_journal-2520	173	21	,	,	PUNCT
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american_scientific_journal-2520	173	23	,	,	PUNCT
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american_scientific_journal-2520	173	25	,	,	PUNCT
american_scientific_journal-2520	173	26	a.	a.	PROPN
american_scientific_journal-2520	173	27	,	,	PUNCT
american_scientific_journal-2520	173	28	&	&	CCONJ
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american_scientific_journal-2520	173	30	,	,	PUNCT
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american_scientific_journal-2520	174	1	parathyroid	parathyroid	PROPN
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american_scientific_journal-2520	174	3	gene	gene	NOUN
american_scientific_journal-2520	174	4	polymorphism	polymorphism	NOUN
american_scientific_journal-2520	174	5	and	and	CCONJ
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american_scientific_journal-2520	174	7	idiopathic	idiopathic	ADJ
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american_scientific_journal-2520	174	9	.	.	PUNCT
american_scientific_journal-2520	175	1	journal	journal	NOUN
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american_scientific_journal-2520	175	7	,	,	PUNCT
american_scientific_journal-2520	175	8	89(10	89(10	PROPN
american_scientific_journal-2520	175	9	)	)	PUNCT
american_scientific_journal-2520	175	10	,	,	PUNCT
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american_scientific_journal-2520	175	12	,	,	PUNCT
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american_scientific_journal-2520	175	14	.	.	PUNCT
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american_scientific_journal-2520	176	5	,	,	PUNCT
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american_scientific_journal-2520	176	7	,	,	PUNCT
american_scientific_journal-2520	176	8	&	&	CCONJ
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american_scientific_journal-2520	176	10	,	,	PUNCT
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american_scientific_journal-2520	176	13	for	for	ADP
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american_scientific_journal-2520	176	15	of	of	ADP
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american_scientific_journal-2520	176	19	in	in	ADP
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american_scientific_journal-2520	176	23	.	.	PUNCT
american_scientific_journal-2520	177	1	asian	asian	PROPN
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american_scientific_journal-2520	177	7	,	,	PUNCT
american_scientific_journal-2520	177	8	2(2	2(2	NUM
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american_scientific_journal-2520	177	10	,	,	PUNCT
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american_scientific_journal-2520	177	12	.	.	PUNCT
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american_scientific_journal-2520	177	14	.	.	PUNCT
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american_scientific_journal-2520	178	2	4	4	NUM
american_scientific_journal-2520	178	3	]	]	X
american_scientific_journal-2520	178	4	marsic	marsic	NOUN
american_scientific_journal-2520	178	5	,	,	PUNCT
american_scientific_journal-2520	178	6	d.	d.	PROPN
american_scientific_journal-2520	178	7	,	,	PUNCT
american_scientific_journal-2520	178	8	hughes	hughes	PROPN
american_scientific_journal-2520	178	9	,	,	PUNCT
american_scientific_journal-2520	178	10	r.	r.	PROPN
american_scientific_journal-2520	178	11	c.	c.	PROPN
american_scientific_journal-2520	178	12	,	,	PUNCT
american_scientific_journal-2520	178	13	byrne	byrne	PROPN
american_scientific_journal-2520	178	14	-	-	PUNCT
american_scientific_journal-2520	178	15	steele	steele	PROPN
american_scientific_journal-2520	178	16	,	,	PUNCT
american_scientific_journal-2520	178	17	m.	m.	PROPN
american_scientific_journal-2520	178	18	l.	l.	PROPN
american_scientific_journal-2520	178	19	,	,	PUNCT
american_scientific_journal-2520	178	20	&	&	CCONJ
american_scientific_journal-2520	178	21	ng	ng	PROPN
american_scientific_journal-2520	178	22	,	,	PUNCT
american_scientific_journal-2520	178	23	j.	j.	PROPN
american_scientific_journal-2520	178	24	d.	d.	PROPN
american_scientific_journal-2520	178	25	pcr	pcr	PROPN
american_scientific_journal-2520	178	26	-	-	PUNCT
american_scientific_journal-2520	178	27	based	base	VERB
american_scientific_journal-2520	178	28	gene	gene	NOUN
american_scientific_journal-2520	178	29	synthesis	synthesis	NOUN
american_scientific_journal-2520	178	30	to	to	PART
american_scientific_journal-2520	178	31	produce	produce	VERB
american_scientific_journal-2520	178	32	recombinant	recombinant	ADJ
american_scientific_journal-2520	178	33	proteins	protein	NOUN
american_scientific_journal-2520	178	34	for	for	ADP
american_scientific_journal-2520	178	35	crystallization	crystallization	NOUN
american_scientific_journal-2520	178	36	.	.	PUNCT
american_scientific_journal-2520	179	1	bmc	bmc	PROPN
american_scientific_journal-2520	179	2	biotechnology	biotechnology	NOUN
american_scientific_journal-2520	179	3	,	,	PUNCT
american_scientific_journal-2520	179	4	8	8	NUM
american_scientific_journal-2520	179	5	,	,	PUNCT
american_scientific_journal-2520	179	6	44	44	NUM
american_scientific_journal-2520	179	7	.	.	NOUN
american_scientific_journal-2520	179	8	2008	2008	NUM
american_scientific_journal-2520	179	9	.	.	PUNCT
american_scientific_journal-2520	180	1	[	[	X
american_scientific_journal-2520	180	2	5	5	NUM
american_scientific_journal-2520	180	3	]	]	SYM
american_scientific_journal-2520	180	4	li	li	PROPN
american_scientific_journal-2520	180	5	,	,	PUNCT
american_scientific_journal-2520	180	6	l.	l.	PROPN
american_scientific_journal-2520	180	7	,	,	PUNCT
american_scientific_journal-2520	180	8	wu	wu	PROPN
american_scientific_journal-2520	180	9	,	,	PUNCT
american_scientific_journal-2520	180	10	l.	l.	PROPN
american_scientific_journal-2520	180	11	p.	p.	PROPN
american_scientific_journal-2520	180	12	,	,	PUNCT
american_scientific_journal-2520	180	13	&	&	CCONJ
american_scientific_journal-2520	180	14	chandrasegaran	chandrasegaran	PROPN
american_scientific_journal-2520	180	15	,	,	PUNCT
american_scientific_journal-2520	180	16	s.	s.	PROPN
american_scientific_journal-2520	180	17	functional	functional	PROPN
american_scientific_journal-2520	180	18	domains	domain	NOUN
american_scientific_journal-2520	180	19	in	in	ADP
american_scientific_journal-2520	180	20	fok	fok	NOUN
american_scientific_journal-2520	180	21	i	i	PRON
american_scientific_journal-2520	180	22	restriction	restriction	NOUN
american_scientific_journal-2520	180	23	endonuclease	endonuclease	NOUN
american_scientific_journal-2520	180	24	.	.	PUNCT
american_scientific_journal-2520	181	1	proceedings	proceeding	NOUN
american_scientific_journal-2520	181	2	of	of	ADP
american_scientific_journal-2520	181	3	the	the	DET
american_scientific_journal-2520	181	4	national	national	PROPN
american_scientific_journal-2520	181	5	academy	academy	PROPN
american_scientific_journal-2520	181	6	of	of	ADP
american_scientific_journal-2520	181	7	sciences	sciences	PROPN
american_scientific_journal-2520	181	8	of	of	ADP
american_scientific_journal-2520	181	9	the	the	DET
american_scientific_journal-2520	181	10	united	united	PROPN
american_scientific_journal-2520	181	11	states	states	PROPN
american_scientific_journal-2520	181	12	of	of	ADP
american_scientific_journal-2520	181	13	america	america	PROPN
american_scientific_journal-2520	181	14	,	,	PUNCT
american_scientific_journal-2520	181	15	1992	1992	NUM
american_scientific_journal-2520	181	16	.	.	PUNCT
american_scientific_journal-2520	182	1	89(10	89(10	PROPN
american_scientific_journal-2520	182	2	)	)	PUNCT
american_scientific_journal-2520	182	3	,	,	PUNCT
american_scientific_journal-2520	183	1	4275–9	4275–9	NOUN
american_scientific_journal-2520	183	2	.	.	PUNCT
american_scientific_journal-2520	184	1	[	[	X
american_scientific_journal-2520	184	2	6	6	NUM
american_scientific_journal-2520	184	3	]	]	SYM
american_scientific_journal-2520	184	4	stemmer	stemmer	NOUN
american_scientific_journal-2520	184	5	,	,	PUNCT
american_scientific_journal-2520	184	6	w.p	w.p	PROPN
american_scientific_journal-2520	184	7	.	.	PROPN
american_scientific_journal-2520	184	8	,	,	PUNCT
american_scientific_journal-2520	184	9	crameri	crameri	PROPN
american_scientific_journal-2520	184	10	,	,	PUNCT
american_scientific_journal-2520	184	11	a.	a.	NOUN
american_scientific_journal-2520	184	12	,	,	PUNCT
american_scientific_journal-2520	184	13	ha	ha	INTJ
american_scientific_journal-2520	184	14	,	,	PUNCT
american_scientific_journal-2520	184	15	k.d	k.d	PROPN
american_scientific_journal-2520	184	16	.	.	PROPN
american_scientific_journal-2520	184	17	,	,	PUNCT
american_scientific_journal-2520	184	18	brennan	brennan	PROPN
american_scientific_journal-2520	184	19	,	,	PUNCT
american_scientific_journal-2520	184	20	t.	t.	NOUN
american_scientific_journal-2520	184	21	m.	m.	NOUN
american_scientific_journal-2520	184	22	and	and	CCONJ
american_scientific_journal-2520	184	23	heyneker	heyneker	PROPN
american_scientific_journal-2520	184	24	,	,	PUNCT
american_scientific_journal-2520	184	25	h.	h.	PROPN
american_scientific_journal-2520	184	26	l.	l.	PROPN
american_scientific_journal-2520	184	27	single	single	ADJ
american_scientific_journal-2520	184	28	-	-	PUNCT
american_scientific_journal-2520	184	29	step	step	NOUN
american_scientific_journal-2520	184	30	assembly	assembly	NOUN
american_scientific_journal-2520	184	31	of	of	ADP
american_scientific_journal-2520	184	32	a	a	DET
american_scientific_journal-2520	184	33	gene	gene	NOUN
american_scientific_journal-2520	184	34	and	and	CCONJ
american_scientific_journal-2520	184	35	entire	entire	ADJ
american_scientific_journal-2520	184	36	plasmid	plasmid	NOUN
american_scientific_journal-2520	184	37	from	from	ADP
american_scientific_journal-2520	184	38	large	large	ADJ
american_scientific_journal-2520	184	39	numbers	number	NOUN
american_scientific_journal-2520	184	40	of	of	ADP
american_scientific_journal-2520	184	41	oligodeoxy	oligodeoxy	ADJ
american_scientific_journal-2520	184	42	ribonucleotides	ribonucleotide	NOUN
american_scientific_journal-2520	184	43	.	.	PUNCT
american_scientific_journal-2520	185	1	gene	gene	NOUN
american_scientific_journal-2520	185	2	.	.	PUNCT
american_scientific_journal-2520	186	1	1995	1995	NUM
american_scientific_journal-2520	186	2	,	,	PUNCT
american_scientific_journal-2520	186	3	16	16	NUM
american_scientific_journal-2520	186	4	;	;	PUNCT
american_scientific_journal-2520	186	5	164(1):4953	164(1):4953	NUM
american_scientific_journal-2520	186	6	.	.	PUNCT
american_scientific_journal-2520	187	1	[	[	X
american_scientific_journal-2520	187	2	7	7	NUM
american_scientific_journal-2520	187	3	]	]	X
american_scientific_journal-2520	187	4	gao	gao	PROPN
american_scientific_journal-2520	187	5	,	,	PUNCT
american_scientific_journal-2520	187	6	x.	x.	PROPN
american_scientific_journal-2520	187	7	,	,	PUNCT
american_scientific_journal-2520	187	8	yo	yo	PROPN
american_scientific_journal-2520	187	9	,	,	PUNCT
american_scientific_journal-2520	187	10	p.	p.	PROPN
american_scientific_journal-2520	187	11	,	,	PUNCT
american_scientific_journal-2520	187	12	keith	keith	PROPN
american_scientific_journal-2520	187	13	,	,	PUNCT
american_scientific_journal-2520	187	14	a.	a.	PROPN
american_scientific_journal-2520	187	15	,	,	PUNCT
american_scientific_journal-2520	187	16	ragan	ragan	NOUN
american_scientific_journal-2520	187	17	,	,	PUNCT
american_scientific_journal-2520	187	18	t.	t.	PROPN
american_scientific_journal-2520	187	19	j.	j.	PROPN
american_scientific_journal-2520	187	20	,	,	PUNCT
american_scientific_journal-2520	187	21	&	&	CCONJ
american_scientific_journal-2520	187	22	harris	harris	PROPN
american_scientific_journal-2520	187	23	,	,	PUNCT
american_scientific_journal-2520	187	24	t.	t.	PROPN
american_scientific_journal-2520	187	25	k.	k.	PROPN
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american_scientific_journal-2520	187	27	balanced	balance	VERB
american_scientific_journal-2520	187	28	inside	inside	NOUN
american_scientific_journal-2520	187	29	-	-	PUNCT
american_scientific_journal-2520	187	30	out	out	ADP
american_scientific_journal-2520	187	31	(	(	PUNCT
american_scientific_journal-2520	187	32	tbio	tbio	ADJ
american_scientific_journal-2520	187	33	)	)	PUNCT
american_scientific_journal-2520	187	34	pcr	pcr	NOUN
american_scientific_journal-2520	187	35	-	-	PUNCT
american_scientific_journal-2520	187	36	based	base	VERB
american_scientific_journal-2520	187	37	gene	gene	NOUN
american_scientific_journal-2520	187	38	synthesis	synthesis	NOUN
american_scientific_journal-2520	187	39	:	:	PUNCT
american_scientific_journal-2520	187	40	a	a	DET
american_scientific_journal-2520	187	41	novel	novel	ADJ
american_scientific_journal-2520	187	42	method	method	NOUN
american_scientific_journal-2520	187	43	of	of	ADP
american_scientific_journal-2520	187	44	primer	primer	NOUN
american_scientific_journal-2520	187	45	design	design	NOUN
american_scientific_journal-2520	187	46	for	for	ADP
american_scientific_journal-2520	187	47	high	high	ADJ
american_scientific_journal-2520	187	48	-	-	PUNCT
american_scientific_journal-2520	187	49	fidelity	fidelity	NOUN
american_scientific_journal-2520	187	50	assembly	assembly	NOUN
american_scientific_journal-2520	187	51	of	of	ADP
american_scientific_journal-2520	187	52	longer	long	ADJ
american_scientific_journal-2520	187	53	gene	gene	NOUN
american_scientific_journal-2520	187	54	sequences	sequence	NOUN
american_scientific_journal-2520	187	55	.	.	PUNCT
american_scientific_journal-2520	188	1	nucleic	nucleic	ADJ
american_scientific_journal-2520	188	2	acids	acid	NOUN
american_scientific_journal-2520	188	3	research	research	NOUN
american_scientific_journal-2520	188	4	,	,	PUNCT
american_scientific_journal-2520	188	5	31(22	31(22	NUM
american_scientific_journal-2520	188	6	)	)	PUNCT
american_scientific_journal-2520	188	7	,	,	PUNCT
american_scientific_journal-2520	188	8	143	143	NUM
american_scientific_journal-2520	188	9	-	-	SYM
american_scientific_journal-2520	188	10	146	146	NUM
american_scientific_journal-2520	188	11	.	.	PUNCT
american_scientific_journal-2520	188	12	2003	2003	NUM
american_scientific_journal-2520	188	13	.	.	PUNCT
american_scientific_journal-2520	189	1	[	[	X
american_scientific_journal-2520	189	2	8	8	NUM
american_scientific_journal-2520	189	3	]	]	X
american_scientific_journal-2520	189	4	rouillard	rouillard	NOUN
american_scientific_journal-2520	189	5	,	,	PUNCT
american_scientific_journal-2520	189	6	j.	j.	PROPN
american_scientific_journal-2520	189	7	m.	m.	PROPN
american_scientific_journal-2520	189	8	,	,	PUNCT
american_scientific_journal-2520	189	9	lee	lee	PROPN
american_scientific_journal-2520	189	10	,	,	PUNCT
american_scientific_journal-2520	189	11	w.	w.	PROPN
american_scientific_journal-2520	189	12	,	,	PUNCT
american_scientific_journal-2520	189	13	truan	truan	PROPN
american_scientific_journal-2520	189	14	,	,	PUNCT
american_scientific_journal-2520	189	15	g.	g.	PROPN
american_scientific_journal-2520	189	16	,	,	PUNCT
american_scientific_journal-2520	189	17	gao	gao	PROPN
american_scientific_journal-2520	189	18	,	,	PUNCT
american_scientific_journal-2520	189	19	x.	x.	PROPN
american_scientific_journal-2520	189	20	,	,	PUNCT
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american_scientific_journal-2520	189	22	,	,	PUNCT
american_scientific_journal-2520	189	23	x.	x.	NOUN
american_scientific_journal-2520	189	24	,	,	PUNCT
american_scientific_journal-2520	189	25	&	&	CCONJ
american_scientific_journal-2520	189	26	gulari	gulari	PROPN
american_scientific_journal-2520	189	27	,	,	PUNCT
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american_scientific_journal-2520	189	29	gene2oligo	gene2oligo	PROPN
american_scientific_journal-2520	189	30	:	:	PUNCT
american_scientific_journal-2520	189	31	oligonucleotide	oligonucleotide	VERB
american_scientific_journal-2520	189	32	design	design	NOUN
american_scientific_journal-2520	189	33	for	for	ADP
american_scientific_journal-2520	189	34	in	in	ADP
american_scientific_journal-2520	189	35	vitro	vitro	X
american_scientific_journal-2520	189	36	gene	gene	NOUN
american_scientific_journal-2520	189	37	synthesis	synthesis	NOUN
american_scientific_journal-2520	189	38	.	.	PUNCT
american_scientific_journal-2520	190	1	nucleic	nucleic	ADJ
american_scientific_journal-2520	190	2	acids	acid	NOUN
american_scientific_journal-2520	190	3	research	research	NOUN
american_scientific_journal-2520	190	4	,	,	PUNCT
american_scientific_journal-2520	190	5	32(web	32(web	NUM
american_scientific_journal-2520	190	6	server	server	PROPN
american_scientific_journal-2520	190	7	iss	iss	PROPN
american_scientific_journal-2520	190	8	.	.	PUNCT
american_scientific_journal-2520	190	9	)	)	PUNCT
american_scientific_journal-2520	190	10	,	,	PUNCT
american_scientific_journal-2520	191	1	176–180	176–180	NUM
american_scientific_journal-2520	191	2	.	.	NOUN
american_scientific_journal-2520	191	3	2004	2004	NUM
american_scientific_journal-2520	191	4	.	.	PUNCT
american_scientific_journal-2520	192	1	[	[	X
american_scientific_journal-2520	192	2	9	9	NUM
american_scientific_journal-2520	192	3	]	]	SYM
american_scientific_journal-2520	192	4	xiong	xiong	PROPN
american_scientific_journal-2520	192	5	,	,	PUNCT
american_scientific_journal-2520	192	6	a.	a.	PROPN
american_scientific_journal-2520	192	7	s.	s.	PROPN
american_scientific_journal-2520	192	8	,	,	PUNCT
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american_scientific_journal-2520	192	10	,	,	PUNCT
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american_scientific_journal-2520	192	12	h.	h.	PROPN
american_scientific_journal-2520	192	13	,	,	PUNCT
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american_scientific_journal-2520	192	15	,	,	PUNCT
american_scientific_journal-2520	192	16	r.	r.	PROPN
american_scientific_journal-2520	192	17	h.	h.	PROPN
american_scientific_journal-2520	192	18	,	,	PUNCT
american_scientific_journal-2520	192	19	li	li	PROPN
american_scientific_journal-2520	192	20	,	,	PUNCT
american_scientific_journal-2520	192	21	x.	x.	PROPN
american_scientific_journal-2520	192	22	,	,	PUNCT
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american_scientific_journal-2520	192	24	,	,	PUNCT
american_scientific_journal-2520	192	25	h.	h.	PROPN
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american_scientific_journal-2520	192	27	,	,	PUNCT
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american_scientific_journal-2520	192	29	,	,	PUNCT
american_scientific_journal-2520	192	30	z.	z.	PROPN
american_scientific_journal-2520	192	31	m.	m.	PROPN
american_scientific_journal-2520	192	32	,	,	PUNCT
american_scientific_journal-2520	192	33	&	&	CCONJ
american_scientific_journal-2520	192	34	li	li	PROPN
american_scientific_journal-2520	192	35	,	,	PUNCT
american_scientific_journal-2520	192	36	y.	y.	PROPN
american_scientific_journal-2520	192	37	a	a	DET
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american_scientific_journal-2520	192	45	-	-	PUNCT
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american_scientific_journal-2520	192	48	-	-	PUNCT
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american_scientific_journal-2520	192	51	-	-	PUNCT
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american_scientific_journal-2520	192	54	synthesis	synthesis	NOUN
american_scientific_journal-2520	192	55	method	method	NOUN
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american_scientific_journal-2520	192	57	long	long	ADJ
american_scientific_journal-2520	192	58	gene	gene	NOUN
american_scientific_journal-2520	192	59	sequences	sequence	NOUN
american_scientific_journal-2520	192	60	.	.	PUNCT
american_scientific_journal-2520	193	1	nucleic	nucleic	ADJ
american_scientific_journal-2520	193	2	acids	acid	NOUN
american_scientific_journal-2520	193	3	research	research	NOUN
american_scientific_journal-2520	193	4	,	,	PUNCT
american_scientific_journal-2520	193	5	32(12	32(12	NUM
american_scientific_journal-2520	193	6	):	):	PUNCT
american_scientific_journal-2520	193	7	98	98	NUM
american_scientific_journal-2520	193	8	-	-	SYM
american_scientific_journal-2520	193	9	104	104	NUM
american_scientific_journal-2520	193	10	.	.	PUNCT
american_scientific_journal-2520	194	1	2004	2004	NUM
american_scientific_journal-2520	194	2	.	.	PUNCT
american_scientific_journal-2520	195	1	[	[	X
american_scientific_journal-2520	195	2	10	10	NUM
american_scientific_journal-2520	195	3	]	]	X
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american_scientific_journal-2520	195	5	,	,	PUNCT
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american_scientific_journal-2520	195	7	m.	m.	PROPN
american_scientific_journal-2520	195	8	,	,	PUNCT
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american_scientific_journal-2520	195	10	,	,	PUNCT
american_scientific_journal-2520	195	11	z.	z.	PROPN
american_scientific_journal-2520	195	12	,	,	PUNCT
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american_scientific_journal-2520	195	14	,	,	PUNCT
american_scientific_journal-2520	195	15	s.	s.	PROPN
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american_scientific_journal-2520	195	17	,	,	PUNCT
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american_scientific_journal-2520	195	27	:	:	PUNCT
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american_scientific_journal-2520	195	29	-	-	PUNCT
american_scientific_journal-2520	195	30	made	make	VERB
american_scientific_journal-2520	195	31	genes	gene	NOUN
american_scientific_journal-2520	195	32	using	use	VERB
american_scientific_journal-2520	195	33	the	the	DET
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american_scientific_journal-2520	195	35	chain	chain	NOUN
american_scientific_journal-2520	195	36	reaction	reaction	NOUN
american_scientific_journal-2520	195	37	.	.	PUNCT
american_scientific_journal-2520	196	1	reprinted	reprint	VERB
american_scientific_journal-2520	196	2	from	from	ADP
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american_scientific_journal-2520	196	4	.	.	PUNCT
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american_scientific_journal-2520	197	2	-	-	SYM
american_scientific_journal-2520	197	3	535	535	NUM
american_scientific_journal-2520	197	4	.	.	PUNCT
american_scientific_journal-2520	198	1	1990	1990	NUM
american_scientific_journal-2520	198	2	.	.	PUNCT
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american_scientific_journal-2520	199	2	11	11	NUM
american_scientific_journal-2520	199	3	]	]	X
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american_scientific_journal-2520	199	5	,	,	PUNCT
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american_scientific_journal-2520	199	8	,	,	PUNCT
american_scientific_journal-2520	199	9	&	&	CCONJ
american_scientific_journal-2520	199	10	pease	pease	PROPN
american_scientific_journal-2520	199	11	,	,	PUNCT
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american_scientific_journal-2520	199	13	r.	r.	PROPN
american_scientific_journal-2520	199	14	gene	gene	PROPN
american_scientific_journal-2520	199	15	splicing	splicing	NOUN
american_scientific_journal-2520	199	16	and	and	CCONJ
american_scientific_journal-2520	199	17	mutagenesis	mutagenesis	NOUN
american_scientific_journal-2520	199	18	by	by	ADP
american_scientific_journal-2520	199	19	pcr	pcr	NOUN
american_scientific_journal-2520	199	20	-	-	PUNCT
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american_scientific_journal-2520	199	23	extension	extension	NOUN
american_scientific_journal-2520	199	24	.	.	PUNCT
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american_scientific_journal-2520	200	2	protocol	protocol	NOUN
american_scientific_journal-2520	200	3	,	,	PUNCT
american_scientific_journal-2520	200	4	2(4	2(4	NUM
american_scientific_journal-2520	200	5	)	)	PUNCT
american_scientific_journal-2520	200	6	,	,	PUNCT
american_scientific_journal-2520	200	7	924–932	924–932	NUM
american_scientific_journal-2520	200	8	.	.	NOUN
american_scientific_journal-2520	200	9	2007	2007	NUM
american_scientific_journal-2520	200	10	.	.	PUNCT
american_scientific_journal-2520	201	1	[	[	X
american_scientific_journal-2520	201	2	12	12	NUM
american_scientific_journal-2520	201	3	]	]	X
american_scientific_journal-2520	201	4	liu	liu	PROPN
american_scientific_journal-2520	201	5	,	,	PUNCT
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american_scientific_journal-2520	201	7	,	,	PUNCT
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american_scientific_journal-2520	201	9	,	,	PUNCT
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american_scientific_journal-2520	201	11	,	,	PUNCT
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american_scientific_journal-2520	201	13	,	,	PUNCT
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american_scientific_journal-2520	201	15	,	,	PUNCT
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american_scientific_journal-2520	201	17	,	,	PUNCT
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american_scientific_journal-2520	201	19	,	,	PUNCT
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american_scientific_journal-2520	201	21	,	,	PUNCT
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american_scientific_journal-2520	201	23	,	,	PUNCT
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american_scientific_journal-2520	201	25	,	,	PUNCT
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american_scientific_journal-2520	201	27	,	,	PUNCT
american_scientific_journal-2520	201	28	&	&	CCONJ
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american_scientific_journal-2520	201	30	,	,	PUNCT
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american_scientific_journal-2520	201	40	1	1	NUM
american_scientific_journal-2520	201	41	-	-	SYM
american_scientific_journal-2520	201	42	84	84	NUM
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american_scientific_journal-2520	201	45	coli	coli	NOUN
american_scientific_journal-2520	201	46	.	.	PUNCT
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american_scientific_journal-2520	202	2	expression	expression	NOUN
american_scientific_journal-2520	202	3	and	and	CCONJ
american_scientific_journal-2520	202	4	purification	purification	NOUN
american_scientific_journal-2520	202	5	,	,	PUNCT
american_scientific_journal-2520	202	6	54(2	54(2	NUM
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american_scientific_journal-2520	202	8	,	,	PUNCT
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american_scientific_journal-2520	202	10	.	.	PUNCT
american_scientific_journal-2520	202	11	2007	2007	NUM
american_scientific_journal-2520	202	12	.	.	PUNCT
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american_scientific_journal-2520	203	3	]	]	PUNCT
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american_scientific_journal-2520	203	5	a.	a.	PROPN
american_scientific_journal-2520	203	6	a.	a.	PROPN
american_scientific_journal-2520	203	7	,	,	PUNCT
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american_scientific_journal-2520	203	9	k.	k.	PROPN
american_scientific_journal-2520	203	10	h.	h.	PROPN
american_scientific_journal-2520	203	11	,	,	PUNCT
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american_scientific_journal-2520	203	13	.	.	PUNCT
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american_scientific_journal-2520	203	20	gene	gene	NOUN
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american_scientific_journal-2520	203	25	.	.	PUNCT
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american_scientific_journal-2520	204	5	,	,	PUNCT
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american_scientific_journal-2520	204	7	.	.	PUNCT
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american_scientific_journal-2520	205	3	]	]	X
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american_scientific_journal-2520	205	5	,	,	PUNCT
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american_scientific_journal-2520	205	8	,	,	PUNCT
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american_scientific_journal-2520	205	10	,	,	PUNCT
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american_scientific_journal-2520	205	13	,	,	PUNCT
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american_scientific_journal-2520	205	18	method	method	NOUN
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american_scientific_journal-2520	205	24	.	.	PUNCT
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american_scientific_journal-2520	207	4	.	.	PUNCT
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american_scientific_journal-2520	207	7	.	.	PUNCT
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american_scientific_journal-2520	208	3	]	]	X
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american_scientific_journal-2520	208	5	,	,	PUNCT
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american_scientific_journal-2520	208	7	,	,	PUNCT
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american_scientific_journal-2520	208	9	,	,	PUNCT
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american_scientific_journal-2520	208	13	&	&	CCONJ
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american_scientific_journal-2520	208	15	,	,	PUNCT
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american_scientific_journal-2520	208	19	design	design	NOUN
american_scientific_journal-2520	208	20	via	via	ADP
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american_scientific_journal-2520	208	22	..	..	PUNCT
american_scientific_journal-2520	208	23	proceedings	proceeding	NOUN
american_scientific_journal-2520	208	24	of	of	ADP
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american_scientific_journal-2520	208	26	2003	2003	NUM
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american_scientific_journal-2520	208	30	,	,	PUNCT
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american_scientific_journal-2520	208	32	,	,	PUNCT
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american_scientific_journal-2520	209	5	,	,	PUNCT
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american_scientific_journal-2520	209	13	to	to	ADP
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american_scientific_journal-2520	209	17	of	of	ADP
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american_scientific_journal-2520	209	19	.	.	PUNCT
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american_scientific_journal-2520	210	8	,	,	PUNCT
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american_scientific_journal-2520	210	10	,	,	PUNCT
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american_scientific_journal-2520	210	20	27	27	NUM
american_scientific_journal-2520	210	21	,	,	PUNCT
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american_scientific_journal-2520	210	23	1	1	NUM
american_scientific_journal-2520	210	24	,	,	PUNCT
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american_scientific_journal-2520	210	26	12	12	NUM
american_scientific_journal-2520	210	27	-	-	SYM
american_scientific_journal-2520	210	28	24	24	NUM
american_scientific_journal-2520	210	29	24	24	NUM
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american_scientific_journal-2520	210	31	,	,	PUNCT
american_scientific_journal-2520	210	32	6(6	6(6	ADJ
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american_scientific_journal-2520	210	34	,	,	PUNCT
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american_scientific_journal-2520	210	37	2011	2011	NUM
american_scientific_journal-2520	210	38	.	.	PUNCT
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american_scientific_journal-2520	211	5	s.	s.	PROPN
american_scientific_journal-2520	211	6	,	,	PUNCT
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american_scientific_journal-2520	211	8	s.	s.	PROPN
american_scientific_journal-2520	211	9	and	and	CCONJ
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american_scientific_journal-2520	211	14	a	a	DET
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american_scientific_journal-2520	211	22	.	.	PUNCT
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american_scientific_journal-2520	212	9	in	in	ADP
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american_scientific_journal-2520	212	11	(	(	PUNCT
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american_scientific_journal-2520	212	15	,	,	PUNCT
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american_scientific_journal-2520	212	20	.	.	PUNCT
american_scientific_journal-2520	213	1	[	[	X
american_scientific_journal-2520	213	2	18	18	NUM
american_scientific_journal-2520	213	3	]	]	X
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american_scientific_journal-2520	213	6	.	.	PUNCT
american_scientific_journal-2520	214	1	the	the	DET
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american_scientific_journal-2520	214	3	of	of	ADP
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american_scientific_journal-2520	214	5	sticky	sticky	ADJ
american_scientific_journal-2520	214	6	end	end	NOUN
american_scientific_journal-2520	214	7	enzymes	enzyme	NOUN
american_scientific_journal-2520	214	8	.	.	PUNCT
american_scientific_journal-2520	215	1	synonym.com	synonym.com	X
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american_scientific_journal-2520	215	4	.	.	PUNCT
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american_scientific_journal-2520	216	5	ajf	ajf	PROPN
american_scientific_journal-2520	216	6	,	,	PUNCT
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american_scientific_journal-2520	216	9	,	,	PUNCT
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american_scientific_journal-2520	216	12	,	,	PUNCT
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american_scientific_journal-2520	216	14	.	.	PUNCT
american_scientific_journal-2520	217	1	an	an	DET
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american_scientific_journal-2520	217	3	to	to	ADP
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american_scientific_journal-2520	217	6	.	.	PUNCT
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american_scientific_journal-2520	218	3	.	.	PUNCT
american_scientific_journal-2520	219	1	w.	w.	PROPN
american_scientific_journal-2520	219	2	h.	h.	PROPN
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american_scientific_journal-2520	219	6	.	.	PUNCT
american_scientific_journal-2520	220	1	2000	2000	NUM
american_scientific_journal-2520	220	2	.	.	PUNCT
american_scientific_journal-2520	221	1	[	[	X
american_scientific_journal-2520	221	2	20	20	NUM
american_scientific_journal-2520	221	3	]	]	PUNCT
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american_scientific_journal-2520	221	5	.	.	PUNCT
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american_scientific_journal-2520	222	3	.	.	PUNCT
american_scientific_journal-2520	223	1	user	user	NOUN
american_scientific_journal-2520	223	2	protocol	protocol	PROPN
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american_scientific_journal-2520	223	5	.	.	PUNCT
american_scientific_journal-2520	224	1	f	f	PROPN
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american_scientific_journal-2520	225	6	.	.	PROPN
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american_scientific_journal-2520	225	8	-	-	PUNCT
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american_scientific_journal-2520	225	10	,	,	PUNCT
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american_scientific_journal-2520	225	12	.	.	PUNCT
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american_scientific_journal-2520	226	2	21	21	NUM
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american_scientific_journal-2520	226	5	,	,	PUNCT
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american_scientific_journal-2520	227	2	,	,	PUNCT
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american_scientific_journal-2520	227	4	.	.	PUNCT
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american_scientific_journal-2520	228	3	]	]	PUNCT
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american_scientific_journal-2520	228	5	,	,	PUNCT
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american_scientific_journal-2520	228	15	protein	protein	NOUN
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american_scientific_journal-2520	228	20	:	:	PUNCT
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american_scientific_journal-2520	228	24	.	.	PUNCT
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american_scientific_journal-2520	229	4	,	,	PUNCT
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american_scientific_journal-2520	229	11	.	.	PUNCT
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american_scientific_journal-2520	230	6	.	.	PUNCT
american_scientific_journal-2520	231	1	tri	tri	ADJ
american_scientific_journal-2520	231	2	reagent	reagent	ADJ
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american_scientific_journal-2520	231	4	.	.	PUNCT
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american_scientific_journal-2520	232	9	,	,	PUNCT
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american_scientific_journal-2520	232	13	.	.	PUNCT
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american_scientific_journal-2520	233	5	,	,	PUNCT
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american_scientific_journal-2520	234	4	,	,	PUNCT
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american_scientific_journal-2520	234	6	.	.	PUNCT
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american_scientific_journal-2520	235	2	.	.	PUNCT
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american_scientific_journal-2520	237	9	-	-	PUNCT
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american_scientific_journal-2520	244	2	from	from	ADP
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american_scientific_journal-2520	244	4	from	from	ADP
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