American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) ISSN (Print) 2313-4410, ISSN (Online) 2313-4402 © Global Society of Scientific Research and Researchers http://asrjetsjournal.org/ Total Coliform and Pollution Index of Water from Domestic Wells in Umalaoge Village-Buton Regency- Southeast Sulawesi-Indonesia Jumadia*, Wa Ode Asfari Azisb, Nursintac a,b,cPublic Health Faculty of Dayanu Ikhsanuddin University, Baubau City 93724, Indonesia a Email: undjumadi@gmail.com bEmail: azizazfari@gmail.com cEmail: sintafkm@gmail.com Abstract Dug Wells have been used as potable water resources of the most Umalaoge Village residents. The study aimed to analysis total coliform and water pollution index of dug wells in Umalaoge Village. Observational research was carried out in rainy season on January 2015. Water samples had been gotten from six dug wells around the village, total sampling. Parameters were taste, color, turbidity, temperature, total dissolved solid, dissolved oxygen, and pH. Total coliform was counted with MPN double tube method. Data should be compared with WHO and national standard. Result of the research show that the all of water wells had total coliform count from 50 to 1898 MPN per 100 ml, exceeded the permissible limit, and water pollution index of dug wells 1,2,3,4, and 5 classified as weak polluted (2.46-2.93) and dug well 6, middle polluted (6.48). Concluded: water generally from those wells is not safe for drinking except some form of treatment is carried out. Keywords: Total Coliform; Pollution Index; Water Dug Well. 1. Introduction In Indonesia, all artificial wells were important source of potable water in some rural developing areas. Dug Well is water reservoir from soil layer closed to the ground surface. ------------------------------------------------------------------------ * Corresponding author. 177 http://asrjetsjournal.org/ https://en.wikipedia.org/wiki/Potable_water American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 15, No 1, pp 177-185 They can be lined with laid stones, extending this lining upwards above the ground surface to form a wall around the well serves to reduce both contaminations. A more modern method called caissoning uses reinforced concrete or plain concrete pre-cast well rings that are lowered into the hole. Umalaoge village, home to some 1.056 people. The most residents have been taken their clean water from dug wells distributing in the village. It is used for drinking, cooking, washing, bathing, and cleaning of their goods. The preliminary observation shows that wells of Umalaoge village were 5.0 to 7.0 m in depths (Table 1). The perception of residents of the rural villages, that the water from the wells is clean. Table 1: Characteristic of Dug wells in Umalaoge Village No. Dug Well Samples Dug Wells Characteristics (metre) H h P D 1. 1 5,0 0,61 2,85 2,90 2. 2 5,5 0,72 3,10 2,23 3. 3 6,1 0,78 3,58 1,26 4. 4 7,0 0,64 4,12 1,65 5. 5 5,0 0,76 2,23 1,11 6. 6 6,5 0,71 3.35 1,47 Note: H (wellline high) , h (wellhead high), P(distance between dug wellhead and dug well surface), D (diameter of dug well surface) Poor well location, construction, hygiene and sanitary practices were among the factors affecting water quality. [1]. reference [2] revealed that in Valley, Colorado that 71% of the wells less than 199 feet (60.7 m) tested positive for total coliform and wells classified as having poor and fair wellhead protection tested positive for total coliform. There for wellhead protection and well depth does play a role in bacterial contamination of water wells. According to Tsega, Sahile [3] the water sources were heavily contaminated which suggested poor protection and sanitation practice in the water sources from open dug wells. There is an urgent need for education of inhabitants on effective water disinfection strategies and for regular monitoring of wells. Temperature, pH, and turbidity, together with the infiltration and percolation of surface water, which takes place in the rainy season, seemed to be the driving factors in the shaping and selection of the bacterial community and deterioration of water quality [4]. The risk of bacterial contamination was lower in wells surrounded by a frame and higher during the rainy season. Well contamination with Salmonella poses a potential threat of infection, thus highlighting the important role of drinking water safety in infectious disease control [5] and it does not to be treated at home so it’s easy to affect waterborne diseases (e.g., cholera, typhus, dysentery, typhoid, diarrhea, and poison of chemical toxicity). This study was carried out aimed analysis total coliform and water pollution index of dug wells in Umalaoge Village. 178 https://en.wikipedia.org/w/index.php?title=Laid_stone&action=edit&redlink=1 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 15, No 1, pp 177-185 2. Methodology 2.1. Location and Time Observational research was carried out in Umalaoge Village-South Lasalimu District-Buton Regency-Southeast Sulawesi-Indonesia in rainy season on January 2015. Figure 1: Location of Dug wells samples in Umalaoge Village 2.2. Material and Methods Research was carried out aimed analysis total coliform and pollution index of water dug wells in Umalaoge Village. Six Water samples from dug wells were taken used Bottle La Motte, pouring to sterile bottle (500 ml) and putted in cooler boxes at 2-4oC for transportation to laboratory for microbial examination. According to [6], that water samples can be stored at ambient temperatures for 12 hours without significantly affecting the coliform counts. Meanwhile physical and chemical parameters had direct examination at the location after samples collected, and total coliform had counted through MPN double tube method incubating at 35oC for 24 hour. The analysis were carried out according to standard methods of water examination and as reported in World Health Organization (WHO) guide limit for water drinking [7]. STORET-USEPA Method was used to analysis water pollution index [8].The results were compared with the values stipulated by WHO, and national standard: 0 < PIj < 1.0 (normal standard water quality), 1.0 < PIj < 5.0 (weak pollution), 5.0 < PIj < 10 (middle pollution), and PIj > 10 (strong pollution) [8]. 3. Results 3.1. Odor and Taste According to opinion of residents that a water dug wells in Umalaoge Village had odor and two of six dug wells had tasted and others tasteless and odorless. 2.3. Color Colors of the water dug wells in Umalaoge Village were ranging from 10 to15 TCU (Figure 2). 2.4. Turbidity 179 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 15, No 1, pp 177-185 Turbidity of the water dug wells in Umalaoge Village were ranging from 1.06 - 7.70 NTU (Figure 3) Figure 2: Color value of the water dug wells in Umalaoge Village Figure 3: Turbidity of the water dug wells in Umalaoge Village 3.4 Temperature Temperature of the all water dug wells in Umalaoge Village were 27oC, 26oC, 27oC, 26oC, 28oC and 27oC respectively. 3.5 Total Dissolved Solid (TDS) Total Dissolved Solid (TDS) of the water of the dug wells in Umalaoge Village were lowest 380 and highest 4750 mg/l (Figure 4). 3.6 Dissolved Oxygene (DO) Dissolved oxygen of the water dug wells in Umalaoge Village were ranging from 7.80 to 8.60 mg/l (Figure 5) 3.7 Power of Hydrogene (pH) pH of the water dug wells in Umalaoge Village were ranging from 6.89 to 7.39 (Figure 6). 180 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 15, No 1, pp 177-185 Figure 4: TDS of the water dug wells in Umalaoge Village Figure 5: DO of the water dug wells in Umalaoge Village Figure 6: pH of the water dug wells in Umalaoge Village 4. Discussion Open dug wells could be poor water quality due to ingress pollutant from surface or subsurface ground. Result 181 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 15, No 1, pp 177-185 studied show that water pollution index of all dug wells in Umalaoge village, viz. 2.61, 2.46, 2.47, 2.93, 2.50, and 6.48 respectively and classified as weak to middle pollution. Construction in poor location, and hygiene and sanitary practices were among the factors affecting water quality of dug wells in Umalaoge Village. The open dug well have been location near to waste water disposal, septic tank from household increased risk contamination, mainly open dug wellheadless and poor lining construction (Figure 7). Table 2: Value of parameters, water quality standard, total coliform, and pollution index of the water dug wells in Umalaoge Village No. Parameters Units Water Quality Standard Value of Parameters Dug Wells 1 2 3 4 5 6 1 Odor - odorless odor odorless odorless odorless odorless odor 2 TDS mg/L 1500 4750** 520 380 420 1030 410 3 Turbidity NTU 25 7.7 1.09 1.06 2.84 3.61 7.65 4 Taste - tasteless taste tasteless tasteless tasteless tasteless taste 5 Temperature ⁰C 26-29 27 26 27 26 28 27 6 Color TCU 50 15 10 10 15 10 10 7 pH - 6.5-9.0 6.89 7.34 7.34 7.22 7.29 7.39 8 DO mg/L 6 8.50 8.00 8.50 7.80 8.60 8.40 9 Total Coliform (MPN) MPN/ 100 ml 50 68** 50 99** 190** 113** 1898** Water Pollution Index 2.61a 2.46 a 2.47 a 2.93 a 2.50 a 6.48 ab Mark: ** = Exceeded the permissible limit a = Weak polluted ab = Middle polluted In Umalaoge Village, the dug wells have various permeability from low, moderate to high permeability sub- soils. Furthermore, well liner clearance was a significant factor in all models, indicating that direct surface ingress is a significant well contamination mechanism. Water quality of dug wells in Umalaoge Village had been influenced surface water ingress to hole of wells which take place in the rainy season. TDS of first dug well has exceeded the desirable limit i.e. 4750 mg/l, open dug wells 2,3,4,5,6 had 520, 380, 420, 1030, and 410 mg/l respectively. High concentration of TDS in the first dug well than others could be influenced by water quality on surface and subsurface around the dug wells. The [9] revealed that in Nagpur district, water quality on surface and subsurface had TDS exceeding the desirable limit i.e. 500 mg/l, related to out of which one dug well from Parsodi exceeded the permissible limit--2000 mg/l. Fluoride concentration exceeded desirable limit 182 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 15, No 1, pp 177-185 1.0 mg/l. Nitrate exceeded desirable limit of (45 mg/l), bore well from Salwa exceeded the permissible limit-- 100 mg/l. Dug wells from Parsodi had total hardness exceeded permissible limit (600 mg/l). Maximum hardness (3.582 mg/l), chloride (1.716 mg/l) and magnesium (572 mg/l). Others factors could influence drinking water quality, viz. pH, electrical conductivity, total dissolved solids, total alkalinity, total hardness, Cl-, SO4 (2-), Ca2+, Mg2+, Na+ and K+ [10]. A B Figure 7: The Dug well construction in Umalaoge Village (A: lined with laid stones; B: casting well rings that are lowered into the hole) Various water quality parameters of open dug wells in Umalaoge Village had been measured, viz. odor, color, turbidity, taste, temperature, pH, total dissolved oxygen,. Result show that just dug wells 1 and 6 had odor and tasted, unsuitable as clean water resources. Turbidity, temperature, pH, and total dissolved oxygen had in range permissible limit. According to Machado and Bordalo [4] that temperature, pH and turbidity, together with the infiltration and percolation of surface water, which takes place in the wet season, seemed to be the driving factors in the shaping and selection of the bacterial community and deterioration of water quality. Total coliform count ((indicator of poor sanitary quality) of water dug wells in Umalaoge was investigated. A total coliform of water dug wells had high counted, viz. 68, 99, 190, 133 and 1898 MPN/100 ml, exceeded permissible limit, and just two dug wells had in permissible limit (50 MPN/100 ml). Bacteriological contamination of the all dug wells in the Umalaoge Village could be due to near to septic tanks, shallow depth, and improper construction of wells. According to Mile, Jande [11] that bacteriological contamination of well water could be due to improper construction of wells, refuse dumping sites and various human activities around the wells. The shallow depth, water logging and presence of septic tanks in the near vicinity are the possible reasons of poor microbial quality of hand pump drinking water. Escherichia coli, Klebsiella sp., Enterobacter sp., Shigella sp. and Salmonella sp. could be identified. The presence of enteric pathogens in water sources pose threat to human health [12]. In Papua New Guinea, individuals in villages using Sepik River water as their primary water source had significantly higher incidence of diarrhea than those primarily using other water sources (streams, dug wells and catchments) [13]. Sanitary practices of residents could influences quality of environment around the dug wells in Umalaoge Village. Garbage and waste water disposal from household, agriculture, and cattle were significantly affect to surface water quality and water of dug wells. According to Hynds, Misstear [14] that on-site septic tank 183 https://en.wikipedia.org/w/index.php?title=Laid_stone&action=edit&redlink=1 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 15, No 1, pp 177-185 presence was the only risk factor significantly associated with bacterial presence in hand dug wells and point agriculture adjacency was significantly associated with water quality of dug well. Poor sanitary practices of residents when using water of dug wells were other factors affecting water quality in Umalaoge Village. Bathing, washing dresses and other goods near the headwells potentially increase poor water quality of dug wells. According to Okotto-Okotto, Okotto [15] that water quality trends were complex, but nitrate levels were related to both sanitary risks and rainfall. 5. Conclusion Discussion with residents about useful water dug wells as drinking and non-drinking domestic uses (e.g., cooking, washing of plates, cups, cutleries etc.) was evident. While the total coliform counts and pollution index reflected the unsanitary habits of the well users. Therefore, water generally from these wells is not safe for drinking except some form of treatment is carried out. So there is need for social discourse and more public health campaign aimed at transforming habits in sanitary practices. Regular monitoring of groundwater quality, abolishment of unhealthy waste disposal practices and introduction of modern techniques are recommended. References [1] Akoachere, J.F., L.A. Omam, and T.N. Massalla, Assessment of the relationship between bacteriological quality of dug-wells, hygiene behaviour and well characteristics in two cholera endemic localities in Douala, Cameroon. BMC Public Health, 2013. 13: p. 692. [2] Gonzales, T.R., The effects that well depth and wellhead protection have on bacterial contamination of private water wells in the Estes Park Valley, Colorado. J Environ Health, 2008. 71(5): p. 17-23. [3] Tsega, N., et al., Bacteriological and physico-chemical quality of drinking water sources in a rural community of Ethiopia. Afr Health Sci, 2013. 13(4): p. 1156-61. [4] Machado, A. and A.A. Bordalo, Analysis of the bacterial community composition in acidic well water used for drinking in Guinea-Bissau, West Africa. J Environ Sci (China), 2014. 26(8): p. 1605-14. [5] Dekker, D.M., et al., Drinking water from dug wells in rural ghana--salmonella contamination, environmental factors, and genotypes. Int J Environ Res Public Health, 2015. 12(4): p. 3535-46. [6] Ahammed, M.M., Effect of holding time and temperature on bacterial counts. Indian J Environ Health, 2003. 45(3): p. 209-12. [7] WHO., Guidelines for drinking-water quality (Surveillance and control of community supplies) at 2nd Ed. Geneva, 1997. Vol. 3: p. 51-94. [8] MNLH, Guideline For Water Quality Assesment. State Ministry of Life Environment of Republic Indonesia, 2003. [9] Khadse, G.K., S.P. Andey, and M.V. Nanoti, Rural water supply and need for water quality assessment: a case study. J Environ Sci Eng, 2005. 47(3): p. 242-9. [10] Muthulakshmi, L., A. Ramu, and N. Kannan, Assessment of groundwater quality in Virudhunagar district (India): a statistical approach. J Environ Sci Eng, 2010. 52(3): p. 229-34. [11] Mile, II, J.A. Jande, and B.I. Dagba, Bacteriological contamination of well water in Makurdi town, Benue State, Nigeria. Pak J Biol Sci, 2012. 15(21): p. 1048-51. 184 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 15, No 1, pp 177-185 [12] Kumar, S., V.R. Tripathi, and S.K. Garg, Physicochemical and microbiological assessment of recreational and drinking waters. Environ Monit Assess, 2012. 184(5): p. 2691-8. [13] Horak, H.M., et al., Microbial and metal water quality in rain catchments compared with traditional drinking water sources in the East Sepik Province, Papua New Guinea. J Water Health, 2010. 8(1): p. 126-38. [14] Hynds, P., et al., Groundwater source contamination mechanisms: physicochemical profile clustering, risk factor analysis and multivariate modelling. J Contam Hydrol, 2014. 159: p. 47-56. [15] Okotto-Okotto, J., et al., A longitudinal study of long-term change in contamination hazards and shallow well quality in two neighbourhoods of Kisumu, Kenya. Int J Environ Res Public Health, 2015. 12(4): p. 4275-91. 185