15 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) ISSN (Print) 2313-4410, ISSN (Online) 2313-4402 © Global Society of Scientific Research and Researchers http://asrjetsjournal.org/ Synthesis, Characterization, Antibacterial, Antifungal and Irritant Activities of Organometalic (ll) Complexes of O- Nitro N,N-Dimethylbenzylamine Derivatives Iram Khana*, Zaid Mahmoodb, Maham Nazc, Tariq Mehmood Buttd aInstitute of Chemistry, University of Punjab,lahore.Pakistan ,54000. bInstitute of Chemistry ,University of Punjab,Lahore. cInstitute of Chemistry, Government college ,Lahore. dbutt Institute of Chemistry ,University of Punjab,Lahore. aE-mail: ikjadoon@hotmail.com bE-mail: zm2-2000@yahoo.com cE-mail: tariqbutt.edu.pk@gmail.com dE-mail: mahamnaz@yahoo.com Abstract A series of Transition metal complexes have been synthesized by reacting with newly prepared biological active Schiff base ligand .The ligand was prepared by reacting N-bromosuccinimide with O-nitro toluene and reflux for 8hours ,resulting mixture was filtered ,filtrate was o-nitro benzyl bromide which on reaction with dimethyl ammine produce o-nitro N,N-dimethylbenzylamine and characterized by elemental analysis, molar conductivity ,thermal analysis ,X-ray diffraction,IR,UV-Vis spectroscopy and mass spectra. Analytical data confirms the ratio between metal and ligand that is 1:2with octahedral geometry. The IRspectra suggests that ligand behaves as basic bidenate ligand. Molar conductance values suggests non electrolytic nature of metal complexes ,thermal behavior shows more ordered activated state in complex form. Antibacterial and Anti-fungal activities were performed by using new strains of bacteriaBacillussubtilis,Bacilluspumilus,Sarcinalutea,Streptococcusfaecalis,Staphlococcusaureus,Borditellabron chiseptica and fungal strains used were Trichophyton longiusus, Candida albicans, Aspergillus flavis,Microsporum canis, Fusarium solani, Candida glabrata. ------------------------------------------------------------------------ * Corresponding author. http://asrjetsjournal.org/ American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 23, No 1, pp 15-28 16 The biological activities data showed that complexes of Copper, Chromium, Manganese and zinc exhibited more antimicrobial activities than their parent ligand. Maximum antibacterial activity was exhibited by zinc complex. Moderate antibacterial activity exhibited by copper complex and the minimum antibacterial response was reported with manganese. Keywords: Bidenate Ligand; Transition Metal complexes; Antimicrobial activity; Disc diffusion method; Spectral studies; Irritant activity. 1. Introduction The schiff base ligands and their metal complexes has many applications in food industry and in medicine .Polymer sciences, biological sciences and used in liquid crystal devices [1]. Transition metal chelates were considered as biological models having various antibacterial activities [2]. Presence of azo group in metal coordination has number of biological applications and shown many antifungal activities [3]. Aromatic carboxylates behave as a schiff base has number of antipoliovirus activities [4]. The herbal drugs used throughout the world have received greater attention in recent times because of their diversity of curing diseases safety and well help to determine models of binding in solid state and to investigate biological activities [5]. The biological activates of transition metal complexes are potent antibacterial and antioxidant agents [6]. Secondary tolerated remedies when compared with conventional medicines. Development of resistance against antibiotics has further emphasized the necessity of research for new therapeutically effective synthetic analogues efficiency is enhanced upon coordination with metal ion [7]. Copper atom played structural and catalytic role in several proteins ,solid complexes have been prepared and characterized physiochemical [8]. It has been recognized as important co-factor in biological molecules either as structural template in protein folding or as a Lewis acid catalyst that can readily adopt 4 ,5 or 6 coordination number [9]. Physiochemical and spectral studies of Copper(II) complexes. The complexes have their own importance in pharmaceutical and medical fields [10]. Metal chelates formed by oximes due to their fascinating chemistry as well as anticancer activities [11] It is a well-known hepatotoxic agent in liver tissues. It was examined that the inhibitory effect of the green tea on cadmium chloride induced antioxidant activity in liver [12]. Phenolic content and DNA protecting in lamiacea plants prevent carcinogenesis through scavenging Cd Cl2[13]. It was investigated that heat-shock and cadmium chloride increase the protein level in human promonocyetic cells [14]. Mn complex of nitrogen based ligand showed antibacterial and antifungal activity against the microbial straining [15].Manganese has shown varied positive biological properties in reverting diseased conditioning gastro protective. Properties of MnCl2 an acetic acid. Induced ulcer in Wistar rats. Manganese has antiulcer NiCl2 and anti-secretary properties which are comparable to standard drug cimetidine provide beneficial condition to attack the highest density of proteins to MoS2(molybdenum disulphide) [16].The electronic properties of trivalent lanthanide ions make them luminescent groups with many currently developing application in biotechnology. Lanthanides (Sn,Eu,Th,Dy) used for multiplexing. Er-YAG laser is used for surgical resurfacing. It has ablative properties with water as main chromophore. It was concluded that both proliferation and apoplosis occurred when the laser irradiated the skin [17]. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 23, No 1, pp 15-28 17 2. Experimental Analytical reagent grade chemicals and solvents including copper chloride chromium chloride manganese chloride and zinc chloride, Ethanol ,Toluene were purchased from Sigma-Aldrich, Lab Scan and Acro Organics. All chemical and solvents were used without any further purification. The FT-IR spectra of complex were recorded in the range of 4000-400 cm-1 as KBr pallets on Agilent technologies. The elemental analysis of the complexes was carried out by engaging complexes with elemental analyzer (LECO Truspec micro Sr No. 4021 Model No. 630-200-200). Conductivity measurements were performed on Wescan-212 conductivity meter. Mass spectra were recorded on MAT 8500Finnigan 70eV.Melting points were recorded on MP-D Mitamura Riken Kogyo (Japan) electro thermal apparatus was used. Thermal analysis were performed on SDT Q600 V20.9 Build 20. 2.1 General procedure for the synthesis of ligand Step 1 The ligand was prepared by modification of reported method, N-Bromosuccimide in 250 ml of carbon tetra chloride, o-nitro toluene (0.5mole) and benzoyl peroxide (1 gram) were charged in quick fit flask equipped with water condenser .reflux the mixture for 6 hours ,then it was allowed to cool down and filter off the filtrate was o-nitrobenzylbromide. Step 2 O-nitrobenzylbromide (prepared in step 1) ,sodium bicarbonate (0.5 mol) and distilled water were taken in quick filled flask equipped with water condenser .Dimethylamine (25.3 ml) was added to reaction mixture with the help of separating funnel drop wise. Mixture was again refluxed for 4 hours then it was allowed to cool and filtered off .Transfer the filtrate to the separating funnel where two layers were formed. The orange layer on elaboration gave orange oily liquid which is our required ligand o-nitro N,N- dimethylbenzylamine (yield :23% ) 2.2 General method for the synthesis of metal complexes To hot ethanolic solution of ligand (0.5 ml) was mixed with clear solution of metal chloride (2 mol) in distilled water with constant stirring. The pH of reaction mixture was maintained at 7-8. Mixture was stirred for 3 hours. White solid settled which was filtered and washed ethanol and n-hexane to get product (yield 62%) 3. Results and Discussion The physio-analytical data of ligand and metal complexes is given in ( Table 1).The metal complex solutions in DMSO shows little conductance which supports their non-electrolytic nature. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 23, No 1, pp 15-28 18 Table 1: Physio-analytical data of ligand and metal compounds Compound Mp (0C) Color conductance Ligand(oily) 180(B.P} Orange - Cu-L 235 Green 13.45 Cr-L 265 Brown 15.15 Mn-L 277 Yellow 17.14 Zn-L 196 White 12.20 Table 2: Solubility of metal complexes in different solvents. Sr # Compd/Complex Solubility DMSO Chloroform Ethyl alcohol Mixed solvent system (Ethanol. Toluene. Ethyl acetate) 1 o-nitro-N,N- dimethylbenzyl amine - (L) + + + + 2 Cu –L + + + + 3 Cr-L Slightly soluble Soluble Slightly soluble + 4 Mn-L soluble Soluble Slightly soluble + 5 Zn- L soluble Soluble Slightly soluble + + shows solubility 4. FTIR spectra The FTIR spectrum of ligand shows characteristic bands of at 3000 ,1698, 1602 ,1361 and 1220 cm-1 which may be assigned due to N(CH3)2 which is again confirmed by the bands at 1530 due to the presence of nitro group on dimethylbenzylamine the IR spectra of metal complexes show absence of broad band in the range of American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 23, No 1, pp 15-28 19 3300-3500 region indicates deprotonation on complexation. This is supported by the appearance of bands towards lower wave number with respect to free ligand which denotes that nitrogen of ligand is co ordinate with metal ion. The IR spectra of metal complexes showed new bands in 400-700 region which can be assigned due to M-O and M-N stretching vibration respectively. Figure 1: FTIR spectra of coppercomplex. Figure 2: FTIR spectra of zinc complex. Table 3: Characteristic IR absorption frequencies of Compound in cm-1 due to bending vibrations for CH group. Compound Observed value (cm1) CH(bending) Observed value (cm1) NH( bending) Observed value (cm1) CH(Stretching) Observed value (cm1) NH(stretching) Observed value (cm1) C-N Observed value (cm1) M-N L 1460 1626.90 3106 3279 & 3323 1030 & 1100 Cu-L 1446 1616 3060 3357 1091 & 1139 1520 Cr- L 1467 1629.70 3033 3305& 3336 1109 1544 Mn- L 1464 1621 3035 3307 & 3338 1103 1567 Zn-L 1457 1634 3032 3335&3358 1150 1559 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 23, No 1, pp 15-28 20 Similarly stretching vibrations for CH group were observed at 3106 cm-1, 3060 cm-1, 3033 cm-1 and 3035 cm-1 for ligand and complexes ,respectively. Stretching vibrations for N-H group for ligand and complexes were observed at 3298 & 3323cm-1 , 3357cm-1,3305 & 3336cm-1 and 3307 & 3338cm-1, respectively. Also the stretching vibrations for C-N bond were observed and values observed at 1030 & 1100cm-1, 1091 & 1139cm-1, 1109cm-1 and 1103cm-1 for ligand and complexes respectively. FT-IR vibrations are shown in table 3.. 4.1 Spectroscopic Analysis The U.V analysis was carried to find out max of ligand and metal complexes which showed max at 216 nm , 281.0nm , 268 nm, 274 nm and 370 nm respectively (Table 4). Table 4: Spectrophotometry analysis of complexes Compounds Metal complex Ligand U.V spectra max 281 nm copper complex 268 nm chromium complex 274 nm Manganese complex 370 nm Zinc complex 216 nm 4.2 Elemental analysis of complexes The elemental analysis of complexes shows the percentage of elements present in metal complexes which is compared with values obtained by theoretical yield , atomic absorption spectra showed the percentage of metals present in metal chelates. The elemental analysis show 1:2 (metal: ligand) stoichiometry for complexes. It corresponds well with general formula ML2, where M=Cu(ll),Cr(ll),Mn(ll)and Zn(ll). Table 5: Elemental analysis of complexes Compound Nitrogen (calculated) Sulphur Hydrogen (calculated) Carbon (calculated) % Metal Copper complex 32.454(20.5) Nil 4.6786(4.2) 12.577(11.2) 21.96% Chromium complex 15.96(10.3) Nil 8.03(6.4) 63.86(57.3) 11.5% Manganese complex 24.56(18.6) Nil 6.78(7.2) 54.35(37.34) 33.2% zinc complex 30.25(21.6) Nil 7.34(4.2) 60.35(48.55) 46.8% American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 23, No 1, pp 15-28 21 4.3 Atomic absorption This analysis was carried out by direct method which gave total metal concentration. .Reference standard solution of each metal complex was prepared having concentrations ranges to 2ppm,4ppm,6ppm,8ppm,10ppm.Absorbances of these solutions were measured at the specific wavelength. A graph was plotted between absorbance and concentration of Cu, Cr, Mn and Zn showed a straight line in each case. The concentrations of unknown solutions were calculated from the absorbance of unknown solution by using the standard values Table 6: Atomic absorption values of metal complexes Sr. No Compounds %of metal(Theoretical) (calculated) 1 O-nitro N,N dimethylbenzyl amine copper(ll)chloride 23.24 24.44 2 O-nitro N,N dimethylbenzyl amine chromium(ll)chloride 31.78 32.02 3 O-nitro N,N dimethylbenzyl amine manganese(ll)chloride 48.30 51.72 4 O-nitro N,N dimethylbenzyl amine zinc(ll)chloride 34.45 37.87 4.4 X-ray Powder diffraction analysis The structure of complexes of copper, zinc and manganese were elucidated x-ray diffraction technique. The complexes obtained in powder form were gently grounded in an agate mortar then powder of each complex was deposited in sample holder equipped with silicon zero –background plate. The diffraction data were collected by scans in the 2 𝜃𝜃 range of 20° − 80° by using a advanced refrectometer equipped with Source Cukαx1 = 1.54 °𝐴𝐴 . PANalytical X PERT Pro Voltage = 40 KV current = 40-1mA 𝜃𝜃 = 20° − 80°.Standerd peak search followed by visualizing the intensity we can find a size of crystallite .The diffractogram of Cu complex shows 18 reflections with maxima at 2 theta s equal to 32.798the corresponding d value is 2.4770 A. the diffractogram of Mn complex had 11 reflections with 2 theta is equal to 86.658 0A,corresponding to d value 1.087 0A whereas the diffractogram of Zn complex had fourteen reflections with maxima 2theta values is equal to 23.110A corresponding to d value 3.8450A.The X-ray diffraction pattern of these complexes with respect to major peaks shows intensity 10% greater than indexed by using computer program. Cu complex shows values of lattice constants a=8.364A,b=7.848A,c=7.761A and alpha =gamma=90degrees and beta =118.40degreesand unit cell volume is equal to468.67A3.Mn complex yield values of lattice constants a=14.152A,b=6.156A,c=4.456A also alpha ,beta and gamma is equal to 90degrees and unit cell volume =385.605A3 ,the required crystallographic data of Mn fulfill the requirement for the compound to be ortho rhombic .Zn complex have values of lattice American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 23, No 1, pp 15-28 22 constants at a=14.546A, b=5.732A c=9.940A .alpha and gamma is equal to 90 degrees and beta =97.396, volume of unit cell is equal to97.453A3.the condition required for the compound to be monoclinic were found satisfactory. Figure 4: XRD spectra of zinc complex. Figure5: XRD spectra of copper complex. 4.5 Thermal analysis The TGA of metal complexes indicates the loss in weight in the range from room temperature to 1860C which is equivalent to 3.86 percent it indicates the loss of water, which is shown by endothermic peak. Further decomposition takes place beyond this temperature from 186-4050C.corresponds to loss in mass of 28.54percent in the TG curve which indicates the expulsion of nitro group from the complex. The decomposition occurs in the temperature range 405 -5300C indicates loss of benzyl group, further at temperature range 530-7500C loss of 26.57percent occurs indicating loss of dimethyl ammine. At 7500C horizontal portion of the curve indicates the presence of thermally stable residual metal oxide .the percentage of residual metal oxide was found to be 24.21 which is very close to theoretical value, TGA of Cr(II) complex shows weight loss corresponding to mass Position [°2Theta] 30 40 50 60 70 Counts 0 20 40 60 80 1 Position [°2Theta] 30 40 50 60 70 Counts 0 50 100 21 .22 4 [ °]; 4.1 86 32 [Å ]; 0 .63 0 [ °] 21 .67 9 [ °]; 4.0 99 40 [Å ]; 0 .23 6 [ °] 25 .06 0 [ °]; 3.5 53 47 [Å ]; 0 .31 5 [ °] 26 .49 1 [ °]; 3.3 64 69 [Å ]; 0 .94 5 [ °] 28 .91 4 [ °]; 3.0 87 98 [Å ]; 0 .27 6 [ °] 31 .59 8 [ °]; 2.8 31 61 [Å ]; 0 .31 5 [ °] 33 .02 0 [ °]; 2.7 12 79 [Å ]; 0 .63 0 [ °] 34 .48 0 [ °]; 2.6 01 23 [Å ]; 0 .63 0 [ °] 36 .50 2 [ °]; 2.4 61 66 [Å ]; 0 .63 0 [ °] 40 .83 3 [ °]; 2.2 09 99 [Å ]; 0 .63 0 [ °] 44 .02 9 [ °]; 2.0 56 71 [Å ]; 0 .47 2 [ °] 46 .79 3 [ °]; 1.9 41 46 [Å ]; 0 .47 2 [ °] 59 .48 1 [ °]; 1.5 54 08 [Å ]; 0 .47 2 [ °] 62 .99 0 [ °]; 1.4 74 48 [Å ]; 1 .15 2 [ °] 10 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 23, No 1, pp 15-28 23 loss 58.63 percent. This loss corresponds to loss of coordinated water molecule with ligand part of complex in the range for room temperature to 2350C.Furthur decomposition at 235-5250C loss of 13.50 percent was occurs indicating loss of carbonyl group with chloride part of complex. The end product of decomposition is formation of Chromium Oxide, weight corresponds to 27.18 percent which is equal to theoretical value 27.58..Similarly TGA spectra obtained for manganese complex and zinc complex can be interpreted and shown in table 7 Table 7: Thermal analysis of Cu(ll),Cr(ll),Mn(ll) and Zn(ll) complexes Complexes Massloss%obs(calc) Temperature(0C) Expected nature of decomposition Cu –L 3.86(3.81) 28.54(27.33) 26.57(29.24) 24.21(24.55) 16.63(16.93) R.T-186 186-405 405-530 530-750 750-1000 Loss of water Expulsion of nitro group Loss of benzyl group Dimethylamine with carbonyl part Residue Cr –L 3.45(3.49) 26.43(27.33) 24.92(25.01) 20.53(20.63) 24.21(23.85) R.T-175 175-405 405-498 498-723 723-1000 Loss of moisture Expulsion of nitro group Loss of benzyl group Dimethylamine with carbonyl part Residue Mn-L 58.63(58.75) 13.50(13.61) 27.16(27.47) 120-235 235-525 525-1000 Loss of moisture Loss of benzyl group from dimethylamine Residue Zn –L 10(10.34) 50.56(50.44) 15.00(14.67) R.T-115 215-310 280-330 Loss of moisture Degradation of complex residue American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 23, No 1, pp 15-28 24 Figure 6: TGA spectra of zinc complex 4.6 Mass Spectral Data of organocopper(ll)derivatives of o-nitro N,N Dimethylbenzylamine The main fragment ions observed for ligand as well as for copper and zinc complexes are listed in table. The molecular ion peak for is observed at m/z 257 for o-nitro N,N Dimethylbenzylammine.and at 394 and 428 for copper and zinc .In both the complexes the primary fragmentation is due to loss of dimethylammine,while secondary and tertiary fragmentation is due to fragmentation of nitro group and benzyl group which is in close agreement with thermal analysis . Table8: Mass spectral analysis of ligand and its derivatives with zinc and copper. Compound Base peak(M+) Fragmentations Ligand 255 213,167,153,144,128 Cu-L 480 394,268,222,205,167,122 Zn-L 475 428,305,259,234,189,156 5. Antimicrobial Activity Disc diffusion method was used to test the antibacterial and antifungal activity. Different strains (bacterial and fungal strains) were engaged to check the antibacterial and antifungal activities .The microorganisms were obtained from Agricultural department Laboratory from University of Punjab, Lahore. They were maintained on a slant on nutrient agar in MC- cartnery bottles and stored at 121oC. American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 23, No 1, pp 15-28 25 5.1 Antibacterial activity Different strains were used to test the antibacterial activity The selected strains were Bacillus subtilis, Bacilluspumilus, Sarcinalutea, Streptococcusfaecalis, Staphlococcusaureus, Borditellabronchiseptica. The ligands and metal complexes were employed for antimicrobial activity by using Disc diffusion method. Nutrient agar was mixed in distilled water and dispersed homogenously. Sterilization of the medium was carried out by means of autoclave at 121 oC for 20 min. Medium was treated with Inoculums before it was transferred to Petri plates. Hereafter, filter paper discs were placed parallel on growth medium which contains 100 µL (micro liters) of complexes and ligands, respectively. The incubation of Petri plates was taken for 24 hours at 37 oC for bacterial growth. The complexes and ligands that exhibited the antibacterial activities well, inhibited the growth of bacteria and formed clear zones. Zone reader was employed to measure the inhibition zones in mili meters. The standard drug used was Ampicillin , Cephalexin[18]. 5.2 Antifungal activity Fungal strains were used to test the antifungal activity. The selected strains were Trichophyton longiusus, Candida albicans, Aspergillus flavis, Microsporum canis, Fusarium solani, Candida glabrata. The growth medium was synthesized, sterilized and then transferred to the Petri plates. Petri dishes were incubated for 48 hours at 28 oC for fungus growth. Filter paper discs were cited on growth medium for the growth of fungus. The complexes and ligands were applied up to 100 µL (micro liter) on each disc. Petri plates were then incubated. The complexes and ligands that exhibited the antifungal activities inhibited the growth of fungus and clear zones were produced. The standard drug used in order to compare the antifungal potential of the complexes and ligands was Micanazole, ketoconazol. Table 9: Antibacterial Activity for metal (ll) derivatives of nitro N,N-Dimethylbenzylamine Standard drug ;Ampicillin ,Cephalexin Name of bacteria Cu complex Zone of inhibition(nm) Cr complex Zone of inhibition(nm) Mn complex Zone of inhibition(nm) Zn complex Zone of inhibition(nm) Bacillus subtilis 10 10 10 16 Bacillus pumilus - 10 17 10 Sarcina lutea - 10 10 - Streptococcus faecalis 10 11 12 - Staphylococcus aureus 11 - 11 11 Burdetella bronchiseptica - 12 - 13 American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 23, No 1, pp 15-28 26 Table10: Antifungal Activity for metal (ll) derivatives of nitro N,N-Dimethylbenzylamine Standard drug;Micanazole, ketoconazole Concentration:2ooug/ml. Name of Fungi Cu complex Percent Inhibition Cr complex Percent Inhibition Mn complex Percent Inhibition Zn complex Percent Inhibition Trichophyton longfusun 55.5 40.3 89.4 63.4 Candida albicans 0 0 0 0 Aspergillus flavus 33 0 57 24 Microsporum canis 0 0 60 78 Fusarium solani 0 0 0 23 Candida glaberate 0 0 46 12 Figure 7: Slides showing anti-bacterial screening of metal complex. 6. Determination of Irritant activity Albino rabbit (oryctolagus cuniculus) having weight 1kg from veterinary research institute, Lahore was purchased ,it was fed on animal fodder provided with tapwater, it was used to test the irritant activity of organometallic complex. For testing the irritant activity hairs present on the ears was shaved off and divided in to three parts with the help of marker about 2microliter of different concentration of metal complexes was applied on three portions .The other ear is taken as control .The redness of ear was observed after every American Scientific Research Journal for Engineering, Technology, and Sciences (ASRJETS) (2016) Volume 23, No 1, pp 15-28 27 15minutes and then after 30minutes .The maximum irritancy on rabbit ear that correspond to the ++scale of Hecker in1971 after 24 hours were recorded. Neither acute nor chronic irritant activity has been exhibited by metal complex as earlier reported [18] Results excluded from antibacterial screening is that transition metal complex show high inhibition towards bacteria AcidovoraxTanpon medium inhibition is shown by Burdetellapertussis. The diverse bioactivity help me to synthesize new bioactive compounds having different mode of action. Bacteria present cause serious threats to our environment and causes morbidity. Organomettalic zinc complex is considered as new biologically active compound having great antibacterial activity against pathogenic bacteria. 7. Conclusion Metal complexes of Cu(II) ,Cr(II),Mn(II),and Zn(II) were synthesized, by using a new ligand obtained by condensation condensation of ortho nitrobenzylbromide, with Dimethylamine in the presence of sodium bicarbonate yield o-nitro N,N-Dimethylbenzylamine .Ligand formed bidenate complexes having octahedral geometry. These complexes were characterized by different physiochemical and spectroscopic techniques. The biological activity shows that zinc complex has shown maximum antibacterial activity .Cu complex has shown moderate antibacterial activity against six species of bacteria and .Mn has minimum antibacterial response. Acknowledgment IK is grateful to Institute of Chemistry ,University of Punjab for providing me necessary facilities to perform spectral analysis. I also acknowledge University of Veterinary Sciences to perform antimicrobial and irritant activities of metal complexes. References [1]. Brandt,E.G.,Hellgren,M.,Brinck,T.Bergman,T.and Edholm, Molecular dynamics study of zinc binding to cysteines in a peptide mimic of the alcohol dehydrogenize structural zinc site. Physical chemistry,11(6) 975-998.2009 [2]. Broadley ,M.R. ,White, P.J.,Zelko,I.and Lux ,.Zinc in plants .News Phytologist,173(4)677-702.2007. [3]. Chandra,S. and Kumar ,EPR,IR and electronical spectral studies on Mn(II),Co(II),Ni(II) and Cu(ll) complexes with a new22 membered azamacrocyclic ligand .Spectrochimica Acta Part A Molecular and Bimolecular spectroscopy 60(8-9),1751-1761.2004. [4]. 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Salami A.T Nd ukavb)a N.G, Lyiola T.O Agbola O.F , Olvwole F.S & Olabye S.B Gastroprotective properties of Manganese Chloride on Acetic Acid induced Ulcer in Wister Rats African journal of biomed research , unit vol 17, 109, 117.,2014.