        item: #1 of 41
          id: cord-004592-a3k56ulv
      author: Liu, Xiaoju
       title: SUMO fusion system facilitates soluble expression and high production of bioactive human fibroblast growth factor 23 (FGF23)
        date: 2012-01-17
       words: 5290
      flesch: 47
     summary: Zuo et al., 2005) ; on the other hand, Rosetta(DE3) strain can provide additional tRNAs for E. coli rare codons (AUA, AGG, AGA, CUA, CCC, GGA), thereby increasing FGF23 gene expression. SUMO fusion technology for difficult-to-express proteins Fibroblast growth factor 23 (FGF23) predicts progression of chronic kidney disease: the Mild to Moderate Kidney Disease (MMKD) study Molecular insights into the klotho-dependent, endocrine mode of action of fibroblast growth factor 19 subfamily members Fibroblast growth factor-23 mitigates hyperphosphatemia but accentuates calcitriol deficiency in chronic kidney disease Expression and purification of glutathione transferase-small ubiquitin-related modifiermetallothionein fusion protein and its neuronal and hepatic protection against D-galactose-induced oxidative damage in mouse model Fibroblast growth factor 23 in oncogenic osteomalacia and Xlinked hypophosphatemia FGF-23: More than a regulator of renal phospate handing? Genetic transmission of tumoral calcinosis: autosomal dominant with variable clinical expressivity SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins Comparison of SUMO fusion technology with traditional gene fusion systems: enhanced expression and solubility with SUMO SUMO fusion technology for enhanced protein production in prokaryotic and eukaryotic expression systems Crystal structures of two FGF-FGFR complexes reveal the determinants of ligand-receptor specificity FGF-23 in fibrous dysplasia of bone and its relationship to renal phosphate wasting FGF-23 is a potent regulator of vitamin D metabolism and phosphate homeostasis Expression and purification of human urodilatin by small ubiquitin-related modifier fusion in Escherichia coli High-level expression and purification of soluble recombinant FGF21 protein by SUMO fusion in Escherichia coli Expression and purification of human keratinocyte growth factor 2 by fusion with SUMO Identification of a novel fibroblast growth factor, FGF-23, preferentially expressed in the ventrolateral thalamic nucleus of the brain Increased circulatory level of biologically active fulllength FGF-23 in patients with hypophosphatemic rickets/osteomalacia Expression and purification of SARS coronavirus proteins using SUMO-fusions Acknowledgements We wish to thank Ms Peng Jing for Western blot analysis.
    keywords: expression; fgf23; fig; fusion; phosphate; protein; sumo
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        item: #2 of 41
          id: cord-010914-08omkszy
      author: Morinaga, Kana
       title: Peculiarities of biofilm formation by Paracoccus denitrificans
        date: 2020-01-30
       words: 3993
      flesch: 36
     summary: In this review, we discuss the peculiarities of biofilm formation by P. denitrificans with the aim of deepening the overall understanding of bacterial biofilm formation and functions. key: cord-010914-08omkszy authors: Morinaga, Kana; Yoshida, Keitaro; Takahashi, Kohei; Nomura, Nobuhiko; Toyofuku, Masanori title: Peculiarities of biofilm formation by Paracoccus denitrificans date: 2020-01-30 journal:
    keywords: bacteria; biofilm; cell; cyclic; denitrificans; formation; gmp; paracoccus
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        item: #3 of 41
          id: cord-010991-fp8hljbq
      author: Rather, Shabeer Ahmad
       title: Antibodies generated against dextransucrase exhibit potential anticariostatic properties in Streptococcus mutans
        date: 2020-01-03
       words: 6619
      flesch: 41
     summary: The effect of dextransucrase antibody on the growth of S. mutans was studied under in vitro conditions. The raised antibodies were checked for affinity with dextransucrase using dot blot assay which showed significant reactivity and further validated by immunofluorescence using confocal microscopy which confirmed specific binding of dextransucrase antibody with the cells of S. mutans.
    keywords: antibody; biofilm; caries; dextransucrase; enzyme; formation; igg; mutans; protein; reactivity; serum
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        item: #4 of 41
          id: cord-011012-5mev3otu
      author: Rathore, Abhishek Singh
       title: Production and immunogenicity of Fubc subunit protein redesigned from DENV envelope protein
        date: 2020-03-30
       words: 6735
      flesch: 42
     summary: It was observed by ELISA that Fubc fusion protein elicited higher serum IgG antibody response either in the presence or in absence of Freund’s adjuvant in comparison to the immune response of Fu and bc peptides separately. In this study, an alignment of the domain II amino acid sequences of DENV1-4 envelope proteins spanning residues from 61 to 120 was used to find optimum conserved sequence for the development of Fubc fusion protein (Fig. 1a) .
    keywords: adjuvant; dengue; denv; envelope; et al; fig; fubc; fusion; immune; loop; protein; response; vaccine; virus
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        item: #5 of 41
          id: cord-011147-55whf8md
      author: Sun, Hengchang
       title: Oral delivery of Bacillus subtilis spores expressing Clonorchis sinensis paramyosin protects grass carp from cercaria infection
        date: 2020-01-07
       words: 7866
      flesch: 47
     summary: Our results were coincident with the former reports about immune response in grass carp induced by oral immunization with spore expressing enolase or cysteine protease of C. sinensis Tang et al. 2017) . TNF-α was also proved to be a crucial cytokine involved in immune regulation and mediates the inflammatory responses in grass carp Zhu et al. 2013 ).
    keywords: b.s; carp; cfu; cotc; cspmy; et al; fishes; grass; group; immune; levels; sinensis; spores; subtilis
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        item: #6 of 41
          id: cord-011212-ovjdzyxv
      author: Pan, Qing
       title: Development and application of a novel ELISA for detecting antibodies against group I fowl adenoviruses
        date: 2019-12-14
       words: 3891
      flesch: 33
     summary: However, there are currently no commercial FAdV-I or FAdV-4 ELISA kits in China for detection of antibodies against the pathogenic FAdVs or FAdV-4 specifically. SPF chicken serum as negative control; FAdV-4 positive serum as positive control Fig.
    keywords: adenovirus; antibodies; chickens; elisa; fadv; fadv-4; fowl; serotypes
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        item: #7 of 41
          id: cord-011320-cwvkox29
      author: Puente-Massaguer, Eduard
       title: Integrating nanoparticle quantification and statistical design of experiments for efficient HIV-1 virus-like particle production in High Five cells
        date: 2020-01-06
       words: 7177
      flesch: 40
     summary: So far, VLP production studies have focused either on the former approach (Krammer et al. 2010; Pushko et al. 2017) or on simple factorial designs (Pillay et al. 2009; Pastor et al. 2019) , limiting the analysis of higher order effects between the variables influencing VLP production. To date, most of the studies dealing with VLP production by recombinant baculovirus infection utilize indirect detection or quantification techniques that hinder the appropriate characterization of the process and product.
    keywords: baculovirus; cell; gagegfp; infection; moi; optimum; particles; production; quantity; vlp; vlps
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        item: #8 of 41
          id: cord-012054-bpgb7tgo
      author: Ferreira, Maria Isabel M.
       title: Degradation of 4-fluorophenol by Arthrobacter sp. strain IF1
        date: 2008-03-01
       words: 5273
      flesch: 45
     summary: Enzyme activitiesTo test inducibility of enzymes involved in 4-FP degradation, cells of Arthrobacter sp. strain IF1 were grown on glucose or 4-FP, washed, and tested for oxygen consumption in the presence of different substrates. When the supernatant of IF1 cells growing on 4 mM 4-FP were subjected to HPLC analysis, four peaks were detected (Table 1) .
    keywords: arthrobacter; catechol; cells; degradation; growth; hydroquinone; hydroxyquinol; if1; strain
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        item: #9 of 41
          id: cord-012056-8b3xffsh
      author: Maas, Ronald H. W.
       title: Lactic acid production from lime-treated wheat straw by Bacillus coagulans: neutralization of acid by fed-batch addition of alkaline substrate
        date: 2008-04-01
       words: 5188
      flesch: 41
     summary: In this study, lime-treated wheat straw was hydrolyzed and fermented simultaneously to lactic acid by an enzyme preparation and Bacillus coagulans DSM 2314. Lactic acid (40.7 g/l) indicated a fermentation efficiency of 81% and a chiral l(+)-lactic acid purity of 97.2%.
    keywords: acid; addition; fermentation; hydrolysis; hydroxide; ltws; phase; sugars
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        item: #10 of 41
          id: cord-012058-ds7u3ke9
      author: Verma, Pradeep
       title: Determination of fungal activity in modified wood by means of micro-calorimetry and determination of total esterase activity
        date: 2008-08-01
       words: 4921
      flesch: 51
     summary: The technology aims at enhancing the durability of wood in terms of resistance against wood decay fungi and insects. Wood mini-blocks were incubated with wood decay fungi according to Bravery (1978) as described above.
    keywords: activity; beech; fungal; fungi; heat; loss; mass; production; versicolor; wood
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        item: #11 of 41
          id: cord-012062-xlw92xoe
      author: Ichinose, Hitomi
       title: Characterization of a modular enzyme of exo-1,5-α-l-arabinofuranosidase and arabinan binding module from Streptomyces avermitilis NBRC14893
        date: 2008-09-01
       words: 4994
      flesch: 38
     summary: The GH43 family incorporates a wide variety of enzyme activities including β-xylosidase (EC 3.2.1.37), α-L-arabinofuranosidase (exo-1,5-α-L-arabinofuanosidase), bifunctional β-xylosidase/α-L-arabinofuranosidase, endo-arabinanase (EC 3.2.1.99), β-xylanase (EC 3.2.1.8), and exo-β-1,3galactanase (EC 3.2.1.145). Because the substrate specificity and the amino acid sequence were quite distinct from known α-L-arabinofuranosidases, it represents a novel type of enzyme as an exo-1,5-α-L-arabinofuranosidase (Matsuo et al. 2000) .
    keywords: activity; arabinan; arabinofuranosidase; debranched; enzyme; residues; saaraf43a; substrate
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        item: #12 of 41
          id: cord-012064-egzl6zk9
      author: Koopman, Frank W.
       title: C(1) compounds as auxiliary substrate for engineered Pseudomonas putida S12
        date: 2009-06-01
       words: 5371
      flesch: 39
     summary: The observed yield increase of the control strain may be attributed to the reducing equivalents generated during formaldehyde oxidation. To further improve this capacity, additional formaldehyde metabolic pathways were constructed by expressing the two key enzymes of the RuMP pathway, 3-hexulose-6-phosphate synthase (Hps) and 6phospho-3-hexulose isomerase (Phi).
    keywords: biomass; cultures; formaldehyde; glucose; methanol; pathway; phosphate; putida; strain; substrate; yield
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        item: #13 of 41
          id: cord-012066-8anhcyia
      author: Stams, Alfons J. M.
       title: Citric acid wastewater as electron donor for biological sulfate reduction
        date: 2009-07-01
       words: 3573
      flesch: 50
     summary: The biological process is operated at low temperature, and a citrate-containing wastewater stream is used as electron donor for biological sulfate reduction. Hydrogen-rich gas is being used as electron donors for biological sulfate reduction at low temperature at full scale (Van Houten et al. 2006;
    keywords: bacteria; citrate; reduction; s101; sludge; strain; sulfate
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        item: #14 of 41
          id: cord-012072-98wuq5ri
      author: Sun, Yvonne
       title: Behavioral response of dissimilatory perchlorate-reducing bacteria to different electron acceptors
        date: 2009-06-17
       words: 5344
      flesch: 35
     summary: Accumulation of nitrite in the suspension suggested the absence of an active nitrite reductase in perchlorate grown cells and also suggested that perchlorate reductase from D. aromatica was responsible for overlapping reduction of (per)chlorate and nitrate. While D. aromatica and D. agitata have perchlorate reductases that reduce nitrate and (per)chlorate, A. suillum, however, has a unique homolog of perchlorate reductase that can potentially distinguish between these electron acceptors and reduce (per)chlorate but not nitrate.
    keywords: acetate; aromatica; electron; nitrate; per)chlorate; perchlorate; response; suillum
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        item: #15 of 41
          id: cord-012085-ubdzhkfq
      author: Jin, Tao
       title: Diversity and quantity of ammonia-oxidizing Archaea and Bacteria in sediment of the Pearl River Estuary, China
        date: 2011-02-01
       words: 4375
      flesch: 50
     summary: AluI was selected to digest PCR products of AOB amoA gene. Similarly, AOB amoA gene resulted in 67 to 333 copies per nanogram DNA and 9.5×10 4 to 6.2×10 5 copies per gram of sediment.
    keywords: abundance; ammonia; amoa; aoa; aob; clone; gene; oxidizing; pcr; primer; sediment
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        item: #16 of 41
          id: cord-012086-sqv56mmq
      author: Meijnen, Jean-Paul
       title: Improved p-hydroxybenzoate production by engineered Pseudomonas putida S12 by using a mixed-substrate feeding strategy
        date: 2011-02-02
       words: 4457
      flesch: 41
     summary: When xylose was co-fed with glucose, the biomass-to-substrate yield slightly increased with increasing xylose concentrations (Fig. 3a) . The effect of xylose co-feeding on p-hydroxybenzoate production was assessed using glucose as the primary Fig.
    keywords: glucose; glycerol; hydroxybenzoate; production; putida; xylose
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        item: #17 of 41
          id: cord-012089-haqqrwad
      author: Baez, Antonino
       title: High-flux isobutanol production using engineered Escherichia coli: a bioreactor study with in situ product removal
        date: 2011-03-10
       words: 4371
      flesch: 49
     summary: In particular, Atsumi et al. (2008b) engineered an E. coli strain (JCL260) as a host for isobutanol production. Since isobutanol is toxic to the cell, isobutanol production from glucose may be limited by the toxicity of the final product itself.
    keywords: coli; fig; growth; isobutanol; jcl260; production
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        item: #18 of 41
          id: cord-012090-pnt4y7zd
      author: Marasabessy, Ahmad
       title: Enhancing Jatropha oil extraction yield from the kernels assisted by a xylan-degrading bacterium to preserve protein structure
        date: 2011-05-10
       words: 5058
      flesch: 47
     summary: Third, we optimized incubation temperature (37, 45, 50, and 55°C) for Jatropha oil extraction (Fig. 6c) . Similar studies in Jatropha oil extraction reported previously did not highlight the importance of preserving protein structure during oil extraction process.
    keywords: extraction; jatropha; kernel; mb4; oil; protein; yield
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        item: #19 of 41
          id: cord-012095-vzk2m91v
      author: Kraakman, Norbertus J. R.
       title: Review of mass transfer aspects for biological gas treatment
        date: 2011-06-24
       words: 9573
      flesch: 31
     summary: Considerable effort has been expended in the search of less energy-intensive reactors to further enhance mass transfer rate. The data for capillary reactors were obtained from , who illustrated, with an order of magnitude analysis, that the relation between mass transfer rate and the power input per reactor volume for a capillary reactor can be expressed as k L a % 0:1
    keywords: bioreactors; biotrickling; compounds; et al; gas; gas phase; gas treatment; liquid; mass; mass transfer; phase; rate; reactor; systems; transfer; treatment; water
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        item: #20 of 41
          id: cord-012096-d7e89x03
      author: Zhang, Tong
       title: Ammonia-oxidizing archaea and ammonia-oxidizing bacteria in six full-scale wastewater treatment bioreactors
        date: 2011-06-25
       words: 4144
      flesch: 49
     summary: The neighbor-joining phylogenetic tree of AOB amoA gene sequences was created by MEGA software (Kumar et al. 2008) . Compared with AOB amoA gene OTUs (shown in Fig. 1 ), the diversity of AmoA protein (a total of 26 OTUs) was less than that of amoA gene (43 OTUs) due to codon wobble positions.
    keywords: amoa; aoa; aob; gene; otus; samples; sequences; sludge
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        item: #21 of 41
          id: cord-012098-1nxws0dk
      author: van den Brink, Joost
       title: Fungal enzyme sets for plant polysaccharide degradation
        date: 2011-07-23
       words: 7463
      flesch: 23
     summary: For instance, Trichoderma reesei has a highly efficient set of enzymes involved in cellulose degradation (Martinez et al. 2008; Kubicek et al. 2011) , while Aspergillus species produce many enzymes to degrade pectin (Martens-Uzunova and Schaap 2009). For example, GH5 contains many catalytic activities, including exoglucanases, endoglucanases, and endomannanases (Dias et al. 2004 ).
    keywords: alpha; aspergillus; carbohydrate; cellulose; degradation; enzymes; et al; families; family; fungal; niger; pectin; plant; reesei; residues; substrate
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        item: #22 of 41
          id: cord-012099-fveq5c9w
      author: Chao, Yuanqing
       title: Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy
        date: 2011-09-01
       words: 4909
      flesch: 38
     summary: Organization of the mycobacterial cell wall: a nanoscale view Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells Nanoscale structural and mechanical properties of nontypeable Haemophilus influenzae biofilms Physical morphology and surface properties of unsaturated Pseudomonas putida biofilms Comparative studies of bacteria with an atomic force microscopy operating in different modes Microbial surfaces investigated using atomic force microscopy Observation of changes in bacterial cell morphology using tapping mode atomic force microscopy Effect of fixation protocols on in situ detection of L-selectin ligands Macromolecular changes caused by formalin fixation and antigen retrieval Atomic force microscopy measurement of heterogeneity in bacterial surface hydrophobicity Atomic force microscopy of microbial cells: application to nanomechanical properties, surface forces and molecular recognition forces Immobilization and molecular interactions between bacteriophage and lipopolysaccharide bilayers Estimation of three-dimensional atomic force microscope tip shape from atomic force microscope image for accurate measurement Effects of acetone, methanol, or paraformaldehyde on cellular structure, visualized by reflection contrast microscopy and transmission and scanning electron microscopy Imaging flagella of Halobacteria by atomic force microscopy Formaldehyde, formalin, paraformaldehyde and glutaraldehyde: what they are and what they do Characterization of type 1 pili of Salmonella typhimurium LT2 Atomic force microscopy in imaging of viruses and virus-infected cells AFM of bacterial cells subjected to different factors Conformational change of flagellin for polymorphic supercoiling of the flagellar filament Probing nanostructures of bacterial extracellular polymeric substances versus culture time by Raman microspectroscopy and atomic force microscopy Studies of chemical fixation effects in human cell lines using Raman microspectroscopy Correlated atomic force microscopy and fluorescence lifetime imaging of live bacterial cells Atomic force microscopy of BHK-21 cells: an investigation of cell fixation techniques Molecular architecture of bacterial flagellum key: cord-012099-fveq5c9w authors: Chao, Yuanqing; Zhang, Tong title: Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy date: 2011-09-01 journal:
    keywords: afm; cells; et al; fixation; flagella; images; methods; morphology; pili; surface
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        item: #23 of 41
          id: cord-012101-mfgca1ou
      author: Luesken, Francisca A.
       title: Diversity and enrichment of nitrite-dependent anaerobic methane oxidizing bacteria from wastewater sludge
        date: 2011-06-11
       words: 5781
      flesch: 54
     summary: These criteria in combination with alternating oxicanoxic conditions present in all WWTPs might reflect the niche of M. oxyfera bacteria where both methane and oxidized nitrogen compounds are present at the same time. The presence of both dissolved methane and oxidized nitrogen compounds (nitrate and nitrite), and the prolonged sludge retention time might favor M. oxyfera bacteria.
    keywords: anaerobic; bacteria; enrichment; et al; lieshout; methane; nc10; nitrite; oxyfera; pcr
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        item: #24 of 41
          id: cord-012430-3uvhoca9
      author: Sanchis, Joaquin
       title: Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates
        date: 2008-11-01
       words: 5541
      flesch: 39
     summary: The ideal methodology for performing site saturation mutagenesis is the one yielding (1) a uniform statistical distribution of the desired mutations (quality criteria) and (2) a number of colonies sufficient to get 95% coverage of the given library in one step (quantity criteria). key: cord-012430-3uvhoca9 authors: Sanchis, Joaquin; Fernández, Layla; Carballeira, J. Daniel; Drone, Jullien; Gumulya, Yosephine; Höbenreich, Horst; Kahakeaw, Daniel; Kille, Sabrina; Lohmer, Renate; Peyralans, Jérôme J.-P.; Podtetenieff, John; Prasad, Shreenath; Soni, Pankaj; Taglieber, Andreas; Wu, Sheng; Zilly, Felipe E.; Reetz, Manfred T. title: Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates date: 2008-11-01 journal:
    keywords: evolution; megaprimer; method; mutagenesis; pcr; quikchange; saturation
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        item: #25 of 41
          id: cord-012431-l2i5utne
      author: Sorokin, D. Y.
       title: Microbiological analysis of the population of extremely haloalkaliphilic sulfur-oxidizing bacteria dominating in lab-scale sulfide-removing bioreactors
        date: 2008-10-01
       words: 4944
      flesch: 42
     summary: Therefore, the feasibility of sulfide oxidation into elemental sulfur under oxygen limitation was tested at extremely haloalkaline conditions in lab-scale bioreactors using mix sediments from hypersaline soda lakes as inoculum. The major principle of the Thiopaq technology is regulation of sulfide oxidation at the level of elemental sulfur by low redox potential, which provides two advantages over the complete oxidation to sulfate: (1) The oxidation does not generate protons but regenerates hydroxyl ions, thereby allowing to save on caustic absorbent; (2) formation of elemental sulfur allows easy separation of the final oxidation product and recirculation of the liquid phase.
    keywords: conditions; genus; oxidation; polysulfide; sob; sulfide; sulfur; thioalkalivibrio; thiosulfate
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        item: #26 of 41
          id: cord-012432-te3sysra
      author: Chmura, A.
       title: Utilization of arylaliphatic nitriles by haloalkaliphilic Halomonas nitrilicus sp. nov. isolated from soda soils
        date: 2008-11-01
       words: 4270
      flesch: 45
     summary: For the fed-batch experiments, 150 μL of Halomonas sp. cell suspension (0.87 mg) was washed and resuspended in 0.1 M phosphate buffer pH 7 to a final volume of 1 mL in a 1.5-mL Eppendorf tube. However, while it is easy to explain for the growing culture, the reason for PAA deficiency in washed cell experiments is not clear.
    keywords: acid; anl; cells; growth; halomonas; nitriles; pan; source; strain
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        item: #27 of 41
          id: cord-012435-tt44dkqd
      author: Temudo, Margarida F.
       title: Diversity of microbial communities in open mixed culture fermentations: impact of the pH and carbon source
        date: 2008-10-01
       words: 5449
      flesch: 41
     summary: Different substrates resulted in the establishment of different microbial communities. key: cord-012435-tt44dkqd authors: Temudo, Margarida F.; Muyzer, Gerard; Kleerebezem, Robbert; van Loosdrecht, Mark C. M. title: Diversity of microbial communities in open mixed culture fermentations: impact of the pH and carbon source date: 2008-10-01 journal:
    keywords: community; culture; et al; glucose; glycerol; microbial; product; substrate; xylose
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        item: #28 of 41
          id: cord-012437-2r6egrca
      author: Lenz, Markus
       title: Bioaugmentation of UASB reactors with immobilized Sulfurospirillum barnesii for simultaneous selenate and nitrate removal
        date: 2009-05-01
       words: 5534
      flesch: 37
     summary: Comparative selenium removal efficiencies were achieved in R2 only after a delay of 5 days (20 HRT; Fig. 1b) . Proliferation of a selenium-respiring population in anaerobic granular sludge of the same origin was also observed when it was operated under methanogenic conditions (Lenz et al. Fig.
    keywords: barnesii; fig; gel; nitrate; period; reactor; removal; selenate; selenium
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        item: #29 of 41
          id: cord-012439-28vi4c2j
      author: Abalakina, Elena G.
       title: Phage Mu-driven two-plasmid system for integration of recombinant DNA in the Methylophilus methylotrophus genome
        date: 2008-11-01
       words: 5430
      flesch: 33
     summary: Constructed helper plasmids with broad-host-range replicons carry thermo-inducible genes for transposition factors MuA and MuB. Integrative plasmids that are only replicated in E. coli could be mobilized to M. methylotrophus and contained mini-Mu unit with a short terminus of Mu DNA, Mu-attL/R. Mini-Mu unit was integrated in the M. methylotrophus genome via mobilization of the integrative plasmid to the cells carrying the helper in conditions of thermo-induced expression of MuA and MuB. In this system, mini-Mu unit was mainly integrated due to replicative transposition, and the integrated copy could be amplified in the M. methylotrophus chromosome in the presence of helper plasmid.
    keywords: dna; km r; methylotrophus; mini; plasmid; transposition; unit
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        item: #30 of 41
          id: cord-012448-9cuq0jqg
      author: Roosen, Christoph
       title: Ionic liquids in biotechnology: applications and perspectives for biotransformations
        date: 2008-12-01
       words: 4446
      flesch: 47
     summary: Highpressure phase behavior of carbon dioxide with imidazoliumbased ionic liquids An analytical view of IL Modelling room temperature ionic liquids Room-temperature IL as replacements for organic solvents in multiphase bioprocess operations Asymmetric biocatalytic reduction of ketones using hydroxyfunctionalised water-miscible IL as solvents Enzymatic reduction of ketones in micro-aqueous media catalyzed by ADH-a from Rhodococcus ruber The effect of IL media on activity, selectivity and stability of candida antarctica lipase b in transesterification reactions Ionic liquids for aqueous two-phase extraction and stabilization of enzymes At low water activity/alpha-chymotrypsin is more active in an IL than in nonionic organic solvents Use of an IL in a twophase system to improve an alcohol dehydrogenase catalysed reduction Maximise equilibrium conversion in biphasic catalysed reactions: mathematical description and practical guideline Enzymatic catalysis of formation of Z-aspartame in IL-an alternative to enzymatic catalysis in organic solvents Enzymatic production of isoamyl acetate in an ionic liquid-alcohol biphasic system Optimization of lipase-catalyzed glucose fatty acid ester synthesis in a two-phase system containing ionic liquids and t-BuOH Enzyme function in organic solvents Lipase-catalyzed enantioselective acylation in the IL solvent system: reaction of enzyme anchored to the solvent Technical aspects of biocatalysis in non-CO 2 -based supercritical fluids Enzymatic selective acylation of glycosides in IL: Significantly enhanced reactivity and regioselectivity Enzymes that work in organic solvents Efficient enantioselective hydrolysis of d,l-phenylglycine methyl ester catalyzed by immobilized Candida antarctica lipase b in IL containing systems Enzymatic resolution of indinavir precursor in IL with reuse of biocatalyst and media by product sublimation Overstabilization of Candida antarctica lipase b by IL in ester synthesis Continuous green biocatalytic processes using IL and supercritical carbon dioxide Chemoenzymatic dynamic kinetic resolution of rac-1-phenylethanol in IL and IL/supercritical carbon dioxide systems Combining reaction calorimetry and ATR-IR spectroscopy for the operando monitoring of IL synthesis IL: efficient additives for candida rugosa lipase-catalysed enantioselective hydrolysis of butyl 2-(4-chlorophenoxy) propionate Purification of imidazolium ionic liquids for spectroscopic applications IL as a new reaction medium for oxidase-peroxidase-catalyzed sulfoxidation Improved preparation and use of roomtemperature IL in lipase-catalyzed enantio-and regioselective acylations Catalysis in IL Increased stability of an esterase from bacillus stearothermophilus in IL as compared to organic solvents Exploring conversion of biphasic catalytic reactions: analytical solution and parameter study Biocatalysis in IL Structure and activity of Candida antarctica lipase b in IL Biocatalysis in IL: batchwise and continuous flow processes using supercritical carbon dioxide as the mobile phase Continuous flow enzymatic kinetic resolution and enantiomer separation using IL/supercritical carbon dioxide media Enzyme catalysis in IL: Comparing IL best results were obtained with lipophilic non-coordinating anions.
    keywords: activity; enzyme; selectivity; solvents; water
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        item: #31 of 41
          id: cord-026729-hn0q0sbv
      author: Xu, Jun
       title: Functional investigation of the chromosomal ccdAB and hipAB operon in Escherichia coli Nissle 1917
        date: 2020-06-13
       words: 8875
      flesch: 45
     summary: Moreover, toxin HipA remarkably reduced the colony-forming activity of E. coli cells (Fig. 1g) . Previous studies showed that biofilm formation promotes the ability of Lactobacillus strains in resistance to temperature, gastric pH and mechanical forces (Salas-Jara et al. 2016) , and a bile-induced biofilm formation during stationary growth allows Bifidobacteria strains for strong colonization in the gastrointestinal tract (Ambalam et al. 2014) .
    keywords: antitoxin; biofilm; ccdab; cells; coli; ecn; escherichia; et al; expression; fig; formation; gene; hipab; persister; response; tass; toxin
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        item: #32 of 41
          id: cord-257136-zpeh8pmc
      author: Huang, Xin
       title: A TaqMan-probe-based multiplex real-time RT-qPCR for simultaneous detection of porcine enteric coronaviruses
        date: 2019-04-26
       words: 3980
      flesch: 46
     summary: To develop a simple, rapid, accurate, and high-throughput detection method for diagnosis and differential diagnosis on swine enteric coronaviruses, specific primers and probes were designed based on the highly conserved regions of transmissible gastroenteritis virus (TGEV) N, porcine epidemic diarrhea virus (PEDV) M, porcine deltacoronavirus (PDCoV) M, and porcine enteric alphacoronavirus (PEAV) N genes respectively. This well-established multiplex real-time RT-qPCR assay provided a rapid, efficient, specific, and sensitive tool for detection of swine enteric coronaviruses.
    keywords: enteric; pdcov; qpcr; swine; tgev; time
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        item: #33 of 41
          id: cord-276575-jfug80yu
      author: Aigner, Achim
       title: Applications of RNA interference: current state and prospects for siRNA-based strategies in vivo
        date: 2007-04-25
       words: 5255
      flesch: 29
     summary: Bradley et al. 2005a, b; Chien et al. 2005; Ge et al. 2004; Hassan et al. 2005; Miyawaki-Shimizu et al. 2005; Morrissey et al. 2005a, b; Schiffelers et al. 2004; Soutschek et al. 2004; Yano et al. 2004; Takeshita et al. 2005 In situ perfusion/intravenous (pancreatic islet) Bradley et al. 2005a , b Intracardiac Bollerot et al. 2006
    keywords: cancer; cells; delivery; et al; gene; molecules; rnai; sirna; small; target; targeting; tumor; vivo
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        item: #34 of 41
          id: cord-282321-svoshzz8
      author: Eboigbodin, Kevin
       title: Reverse transcription strand invasion based amplification (RT-SIBA): a method for rapid detection of influenza A and B
        date: 2016-04-11
       words: 4417
      flesch: 42
     summary: The best performing seeding region was then chosen for subsequent SIBA influenza A experiments and adopted for influenza B SIBA assay design. In order to test our hypothesis, SIBA influenza A assay IOs containing seeding regions that differed in length and nucleotide composition were designed and tested, and the results are depicted in Fig.
    keywords: amplification; assays; detection; influenza; pcr; region; rna; seeding; siba
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        item: #35 of 41
          id: cord-287602-vda01gj6
      author: Jin, Yu-Bei
       title: Immune responses induced by recombinant Lactobacillus plantarum expressing the spike protein derived from transmissible gastroenteritis virus in piglets
        date: 2018-07-18
       words: 6505
      flesch: 41
     summary: In previous reports, this vaccine strategy offered a variety of benefits in that the constructed L. plantarum expression of protein-fused DCpep could activate mucosal DCs, B cells, and T cells to induce the immunological response and could regulate inflammatory responses that took place in a mucosal microenvironment (Steinman and Idoyaga 2010) . Another reasonable explanation is that DCpep may also be recognized by B cells which some B cells also have antigenpresenting function, which subsequently presented the antigens to T cells with APC function.
    keywords: cells; dcpep; expression; lactobacillus; nc8; pgsa; piglets; psip409; recombinant; responses; tgev
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        item: #36 of 41
          id: cord-288673-ku3tmjd3
      author: Sabotič, Jerica
       title: Microbial and fungal protease inhibitors—current and potential applications
        date: 2012-01-05
       words: 14637
      flesch: 17
     summary: In medicine, protease inhibitors can be used as diagnostic or therapeutic agents for viral, bacterial, fungal and parasitic diseases as well as for treating cancer and immunological, neurodegenerative and cardiovascular diseases. Furthermore, protease inhibitors are indispensable in protein purification procedures to prevent undesired proteolysis during heterologous expression or protein extraction.
    keywords: activity; addition; bacteria; binding; cancer; cathepsin; cell; cysteine; cysteine protease; development; diseases; e.g.; et al; expression; families; family; fungal; fungi; host; inhibitors; metalloproteases; microbial; novel; origin; peptidase; peptide; plant; protease; protease inhibitors; protein; rawlings; resistance; role; serine; staphylococcus; structure; target; use; virulence
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        item: #37 of 41
          id: cord-302503-7s9f8wje
      author: Fu, Yuguang
       title: Rapid and efficient detection methods of pathogenic swine enteric coronaviruses
        date: 2020-05-19
       words: 6360
      flesch: 33
     summary: Multiplex nested RT-PCR for the detection of porcine enteric viruses SARS-CoV and emergent coronaviruses: viral determinants of interspecies transmission Field validation of a commercial blocking ELISA to differentiate antibody to transmissible gastroenteritis virus (TGEV) and porcine respiratory coronavirus and to identify TGEV-infected swine herds Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification Interspecies transmission and emergence of novel viruses: lessons from bats and birds Complete genome sequence of a variant porcine epidemic diarrhea virus strain isolated in China Full-length genome characterization of Chinese porcine deltacoronavirus strain CH/ SXD1/2015 Molecular epidemiology of porcine epidemic diarrhea virus in China Pathogenicity and pathogenesis of a United States porcine deltacoronavirus cell culture isolate in 5-day-old neonatal piglets Detection and characterization of group C rotaviruses in asymptomatic piglets in Ireland Development of a multiplex RT-PCR for the detection of major diarrhoeal viruses in pig herds in China Transmissible gastroenteritis virus infection induces NF-kappaB activation through RLR-mediated signaling Porcine deltacoronavirus in mainland China A transmissible gastroenteritis in pigs Transmissible gastroenteritis: demonstration of the virus from field specimens by means of cell culture and pig inoculation SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses Transmissible gastroenteritis Epidemiological survey of porcine epidemic diarrhea virus in swine farms in A new bat-HKU2-like coronavirus in swine Serological evidence of infection with a coronavirus related to transmissible gastroenteritis virus and porcine epidemic diarrhea virus Establishment and application of a multiplex RT-PCR to differentiate wild-type and vaccine strains of porcine epidemic diarrhea virus Detection, sequence analysis, and antibody prevalence of porcine deltacoronavirus in Taiwan Development of a one-step RT-PCR assay for detection of pancoronaviruses (alpha-, beta-, gamma-, and delta-coronaviruses) using newly designed degenerate primers for porcine and avian`fecal samples A TaqManprobe-based multiplex real-time RT-qPCR for simultaneous detection of porcine enteric coronaviruses Porcine deltacoronavirus infection: etiology, cell culture for virus isolation and propagation, molecular epidemiology and pathogenesis Porcine epidemic diarrhea virus infection: etiology, epidemiology, pathogenesis and immunoprophylaxis Viral load quantitation of SARS-coronavirus RNA using a one-step real-time RT-PCR Development of a reverse transcription-nested polymerase chain reaction assay for differential diagnosis of transmissible gastroenteritis virus and porcine respiratory coronavirus from feces and nasal swabs of infected pigs Multiplex real-time RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine epidemic diarrhea virus Differential detection of transmissible gastroenteritis virus and porcine epidemic diarrhea virus by duplex RT-PCR Isolation and serial propagation of porcine epidemic diarrhea virus in cell cultures and partial characterization of the isolate Porcine respiratory coronavirus: molecular features and virus-host interactions Detection and characterization of distinct alphacoronaviruses in five different bat species in Denmark Genetic differentiation of the nucleocapsid protein of Korean isolates of porcine epidemic diarrhoea virus by RT-PCR based restriction fragment length polymorphism analysis Complete genome characterization of Korean porcine deltacoronavirus strain KOR/KNU14-04 Full-length genome sequence of porcine deltacoronavirus strain USA/IA/2014/8734 New variants of porcine epidemic diarrhea virus, China Detection and differentiation of five diarrhea related pig viruses utilizing a multiplex PCR assay A TaqMan probe-based real-time PCR to differentiate porcine epidemic diarrhea virus virulent strains from attenuated vaccine strains Reverse transcription-PCR assays for the differentiation of various US porcine epidemic diarrhea virus strains Origin, evolution, and virulence of porcine deltacoronaviruses in the United States Rapid detection, complete genome sequencing, and phylogenetic analysis of porcine deltacoronavirus A novel pancoronavirus RT-PCR assay: frequent detection of human coronavirus NL63 in children hospitalized with respiratory tract infections in Belgium The first case of porcine epidemic diarrhea in Canada Discovery of a novel swine enteric alphacoronavirus (SeACoV) in southern China Cloning and further sequence analysis of the ORF3 gene of wild-and attenuated-type porcine epidemic diarrhea viruses Detection of transmissible gastroenteritis virus by RT-PCR and differentiation from porcine respiratory coronavirus Isolation of a porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis A new coronavirus-like particle associated with diarrhea in swine Complete genome sequence of transmissible gastroenteritis coronavirus PUR46-MAD clone and evolution of the purdue virus cluster Development of reverse transcription loop-mediated isothermal amplification for rapid detection of porcine epidemic diarrhea virus Rapid detection of swine transmissible gastroenteritis virus by nested polymerase chain reaction Comparative pathogenesis of enteric viral infections of swine Isolation of porcine epidemic diarrhea virus in porcine cell cultures and experimental infection of pigs of different ages Development and optimization of a sensitive TaqMan(R) real-time PCR with synthetic homologous extrinsic control for quantitation of Human cytomegalovirus viral load Porcine epidemic diarrhoea virus: a comprehensive review of molecular epidemiology, diagnosis, and vaccines Newly emerged porcine deltacoronavirus associated with diarrhoea in swine in China: identification, prevalence and full-length genome sequence analysis Differentiation of a Vero cell adapted porcine epidemic diarrhea virus from Korean field strains by restriction fragment length polymorphism analysis of ORF 3 Emergence of porcine epidemic diarrhea virus in the United States: clinical signs, lesions, and viral genomic sequences A novel duplex TaqMan probebased real-time RT-qPCR for detecting and differentiating classical and variant porcine epidemic diarrhea viruses An immunohistochemical investigation of porcine epidemic diarrhoea Epidemiology and vaccine of porcine epidemic diarrhea virus in China: a mini-review Outbreak of porcine epidemic diarrhea in suckling piglets An outbreak of swine diarrhea of a new-type associated with coronavirus-like particles in Japan Development of a TaqMan MGB RT-PCR for the rapid detection of H3 subtype avian influenza virus circulating in China Evidence of recombinant strains of porcine epidemic diarrhea virus Solid phase immune electron microscopy for diagnosis of transmissible gastroenteritis in pigs A real-time TaqMan RT-PCR assay with an internal amplification control for rapid detection of transmissible gastroenteritis virus in swine fecal samples A pancoronavirus RT-PCR assay for detection of all known coronaviruses Reverse transcription cross-priming amplificationnucleic acid test strip for rapid detection of porcine epidemic diarrhea virus Rapid detection of transmissible gastroenteritis virus in swine small intestine samples using real-time reverse transcription recombinase polymerase amplification Detection and genetic characterization of deltacoronavirus in pigs Development and evaluation of a duplex real-time RT-PCR for detection and differentiation of virulent and variant strains of porcine epidemic diarrhea viruses from the United States Simultaneous detection of five pig viruses associated with enteric disease in pigs using EvaGreen real-time PCR combined with melting curve analysis Evidence for a porcine respiratory coronavirus, antigenically similar to transmissible gastroenteritis virus, in the United States Coronavirus genomics and bioinformatics analysis Discovery of seven novel Mammalian and avian coronaviruses in the genus deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of gammacoronavirus and deltacoronavirus Ultrasensitive detection of porcine epidemic diarrhea virus from fecal samples using functionalized nanoparticles Whole-genome analysis of porcine epidemic diarrhea virus (PEDV) from eastern China Genetic variation analysis of reemerging porcine epidemic diarrhea virus prevailing in central China from Development of a nanoparticle-assisted PCR assay for detection of porcine epidemic diarrhea virus Porcine deltacoronavirus: overview of infection dynamics, diagnostic methods, prevalence and genetic evolution Evaluation of two singleplex reverse transcription-Insulated isothermal PCR tests and a duplex real-time RT-PCR test for the detection of porcine epidemic diarrhea virus and porcine deltacoronavirus A multiplex RT-PCR assay for rapid and differential diagnosis of four porcine diarrhea associated viruses in field samples from pig farms in East China from 2010 to 2012 Development of a multiplex TaqMan probe-based real-time PCR for discrimination of variant and classical porcine epidemic diarrhea virus Development of a TaqMan-based real-time RT-PCR assay for the detection of SADS-CoV associated with severe diarrhea disease in pigs Comparison and evaluation of conventional RT-PCR, SYBR green I and TaqMan real-time RT-PCR assays for the detection of porcine epidemic diarrhea virus Development of TaqMan real-time reverse transcription-polymerase chain reaction for the detection and quantitation of porcine kobuvirus A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens Establishment of a nanoparticle-assisted RT-PCR assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus Publisher's note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations In October 2010, a severe PED outbreak caused by a highly virulent PEDV variant emerged in southern China with high mortality ranging from 70 to 100%; the result was devastating damage to the pig farm industry and tremendous economic losses, and later, the PEDV variant spreads to other countries, e.g., USA, Canada, and Mexico ( For the early and rapid detection of PEDV, different types of PCR methods have been developed.
    keywords: assay; detection; diarrhea; et al; gene; pcr; pedv; porcine; time; virus
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        item: #38 of 41
          id: cord-321602-88b2h06y
      author: Lv, Chenfei
       title: Construction of an infectious bronchitis virus vaccine strain carrying chimeric S1 gene of a virulent isolate and its pathogenicity analysis
        date: 2020-08-19
       words: 4933
      flesch: 49
     summary: Construction scheme of a full-length cDNA clone of IBV H120 To construct the full-length cDNA of IBV H120 strain, total RNA was extracted from the allantoic fluid of SPF chicken embryonated eggs infected with H120 and transcribed to cDNA by reverse transcriptase using the Thermo Scientific RevertAid First-Strand cDNA Synthesis Kit (Thermo Fisher, USA). The procedure for the construction of full-length cDNA of IBV H120 has been shown in Fig. 1a .
    keywords: cdna; fig; ibv; infectious; pbac; rh120; scs1; strain; virus
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        item: #39 of 41
          id: cord-339694-sp212tai
      author: Jiang, Xinpeng
       title: A phase trial of the oral Lactobacillus casei vaccine polarizes Th2 cell immunity against transmissible gastroenteritis coronavirus infection
        date: 2016-03-28
       words: 6830
      flesch: 37
     summary: This vaccine could induce TGEV antibody immune responses in both the humoral and mucosal immune systems. The pig model was developed to study intestinal mucosal immune responses (Ruan and Zhang 2013) .
    keywords: cells; et al; expression; group; immune; immunization; infection; lactobacillus; mucosal; pbs; piglets; response; tgev; vaccine
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        item: #40 of 41
          id: cord-348310-nc1tq5af
      author: Vázquez-Ramírez, Daniel
       title: High titer MVA and influenza A virus production using a hybrid fed-batch/perfusion strategy with an ATF system
        date: 2019-02-23
       words: 7472
      flesch: 41
     summary: Modified Vaccinia Virus Ankara (MVA) as production platform for vaccines against influenza and other viral respiratory diseases Viral vaccine production in cell culture Therapeutic vaccination to treat chronic infectious diseases: current clinical developments using MVA-based vaccines The avian EB66® cell line, application to vaccines, and therapeutic protein production The development and manufacture of influenza vaccines Host range and cytopathogenicity of the highly attenuated MVA strain of vaccinia virus: propagation and generation of recombinant viruses in a nonhuman mammalian cell line Phase I clinical trial safety of DNA-and modified virus Ankara-vectored human immunodeficiency virus type 1 (HIV-1) vaccines administered alone and in a prime-boost regime to healthy HIV-1-uninfected volunteers Very high density of CHO cells in perfusionby ATF or TFF in WAVE bioreactor™-Part I. Effect of the cell density on the process Preparation of cell cultures and vaccinia virus stocks Use of cell bleed in a high cell density perfusion culture and multivariable control of biomass and metabolite concentrations High cell density cultivations by alternating tangential flow (ATF) perfusion for influenza A virus production using suspension cells Clinical development of modified vaccinia virus Ankara vaccines Current status for high titre poxvirus stock preparation in CEF under serumfree medium conditions: implication for vaccine development Synergistic DNA-MVA prime-boost vaccination regimes for malaria and tuberculosis MVA and NYVAC as vaccines against emergent infectious diseases and cancer Poxvirus vectors as HIV/AIDS vaccines in humans. The latter differed Similarly, the volumetric productivity (P V ) was calculated considering vir T , the total spent medium during cell growth and virus replication phase (V T, L), and the total process time (t T, day), by: Samples of 6-8 mL from bioreactor cultures were taken with a syringe through a Luer-Lock-septum in 12 or 24-h intervals and stored at − 80°C until analysis.
    keywords: cell; et al; fig; hybrid; influenza; medium; mva; perfusion; production; virus
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        item: #41 of 41
          id: cord-354904-7gq2e6f0
      author: Staroverov, Sergey A.
       title: Prospects for the use of spherical gold nanoparticles in immunization
        date: 2018-11-06
       words: 5061
      flesch: 43
     summary: The degradative processes observed in the animals immunized with TGEV antigen alone are evidence of weak resistance to pathogen attack. These results can be used to develop vaccines against this infection by employing TGEV antigen coupled to gold nanoparticles as a carrier.
    keywords: activity; animals; antigen; cells; gnps; gold; group; immunization; nanoparticles; spleen; tgev; tgev antigen
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