id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
cord-012430-3uvhoca9	Sanchis, Joaquin	Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates	2008-11-01		.txt	text/plain	5541	187	39	The ideal methodology for performing site saturation mutagenesis is the one yielding (1) a uniform statistical distribution of the desired mutations (quality criteria) and (2) a number of colonies sufficient to get 95% coverage of the given library in one step (quantity criteria). key: cord-012430-3uvhoca9 authors: Sanchis, Joaquin; Fernández, Layla; Carballeira, J. Daniel; Drone, Jullien; Gumulya, Yosephine; Höbenreich, Horst; Kahakeaw, Daniel; Kille, Sabrina; Lohmer, Renate; Peyralans, Jérôme J.-P.; Podtetenieff, John; Prasad, Shreenath; Soni, Pankaj; Taglieber, Andreas; Wu, Sheng; Zilly, Felipe E.; Reetz, Manfred T. title: Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates date: 2008-11-01 journal:	cache/cord-012430-3uvhoca9.txt	txt/cord-012430-3uvhoca9.txt
