id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
cord-012439-28vi4c2j	Abalakina, Elena G.	Phage Mu-driven two-plasmid system for integration of recombinant DNA in the Methylophilus methylotrophus genome	2008-11-01		.txt	text/plain	5430	148	33	Constructed helper plasmids with broad-host-range replicons carry thermo-inducible genes for transposition factors MuA and MuB. Integrative plasmids that are only replicated in E. coli could be mobilized to M. methylotrophus and contained mini-Mu unit with a short terminus of Mu DNA, Mu-attL/R. Mini-Mu unit was integrated in the M. methylotrophus genome via mobilization of the integrative plasmid to the cells carrying the helper in conditions of thermo-induced expression of MuA and MuB. In this system, mini-Mu unit was mainly integrated due to replicative transposition, and the integrated copy could be amplified in the M. methylotrophus chromosome in the presence of helper plasmid.	cache/cord-012439-28vi4c2j.txt	txt/cord-012439-28vi4c2j.txt
