id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
cord-321602-88b2h06y	Lv, Chenfei	Construction of an infectious bronchitis virus vaccine strain carrying chimeric S1 gene of a virulent isolate and its pathogenicity analysis	2020-08-19		.txt	text/plain	4933	179	49	Construction scheme of a full-length cDNA clone of IBV H120 To construct the full-length cDNA of IBV H120 strain, total RNA was extracted from the allantoic fluid of SPF chicken embryonated eggs infected with H120 and transcribed to cDNA by reverse transcriptase using the Thermo Scientific RevertAid First-Strand cDNA Synthesis Kit (Thermo Fisher, USA). The procedure for the construction of full-length cDNA of IBV H120 has been shown in Fig. 1a .	cache/cord-321602-88b2h06y.txt	txt/cord-321602-88b2h06y.txt
