id	sid	tid	token	lemma	pos
aiua-14101	1	1	stesura	stesura	PROPN
aiua-14101	1	2	seveso	seveso	PROPN
aiua-14101	1	3	archivio	archivio	PROPN
aiua-14101	1	4	italiano	italiano	X
aiua-14101	1	5	di	di	X
aiua-14101	1	6	urologia	urologia	PROPN
aiua-14101	1	7	e	e	PROPN
aiua-14101	1	8	andrologia	andrologia	NOUN
aiua-14101	1	9	2025	2025	NUM
aiua-14101	1	10	;	;	PUNCT
aiua-14101	1	11	97(3):14101	97(3):14101	NUM
aiua-14101	1	12	1	1	NUM
aiua-14101	1	13	original	original	ADJ
aiua-14101	1	14	paper	paper	NOUN
aiua-14101	1	15	ing	ing	ADJ
aiua-14101	1	16	day	day	NOUN
aiua-14101	1	17	by	by	ADP
aiua-14101	1	18	day	day	NOUN
aiua-14101	1	19	.	.	PUNCT
aiua-14101	2	1	the	the	DET
aiua-14101	2	2	increasing	increase	VERB
aiua-14101	2	3	demand	demand	NOUN
aiua-14101	2	4	to	to	PART
aiua-14101	2	5	use	use	VERB
aiua-14101	2	6	rats	rat	NOUN
aiua-14101	2	7	as	as	SCONJ
aiua-14101	2	8	experimental	experimental	ADJ
aiua-14101	2	9	animals	animal	NOUN
aiua-14101	2	10	has	have	AUX
aiua-14101	2	11	created	create	VERB
aiua-14101	2	12	a	a	DET
aiua-14101	2	13	need	need	NOUN
aiua-14101	2	14	for	for	ADP
aiua-14101	2	15	the	the	DET
aiua-14101	2	16	production	production	NOUN
aiua-14101	2	17	and	and	CCONJ
aiua-14101	2	18	preservation	preservation	NOUN
aiua-14101	2	19	of	of	ADP
aiua-14101	2	20	transgenic	transgenic	NOUN
aiua-14101	2	21	and	and	CCONJ
aiua-14101	2	22	wild	wild	ADJ
aiua-14101	2	23	-	-	PUNCT
aiua-14101	2	24	type	type	NOUN
aiua-14101	2	25	rat	rat	NOUN
aiua-14101	2	26	strains	strain	NOUN
aiua-14101	2	27	.	.	PUNCT
aiua-14101	3	1	the	the	DET
aiua-14101	3	2	best	good	ADJ
aiua-14101	3	3	way	way	NOUN
aiua-14101	3	4	to	to	PART
aiua-14101	3	5	preserve	preserve	VERB
aiua-14101	3	6	these	these	DET
aiua-14101	3	7	gene	gene	NOUN
aiua-14101	3	8	resources	resource	NOUN
aiua-14101	3	9	for	for	ADP
aiua-14101	3	10	many	many	ADJ
aiua-14101	3	11	years	year	NOUN
aiua-14101	3	12	is	be	AUX
aiua-14101	3	13	by	by	ADP
aiua-14101	3	14	freezing	freeze	VERB
aiua-14101	3	15	rat	rat	NOUN
aiua-14101	3	16	embryos	embryo	NOUN
aiua-14101	3	17	,	,	PUNCT
aiua-14101	3	18	sperms	sperm	NOUN
aiua-14101	3	19	,	,	PUNCT
aiua-14101	3	20	and	and	CCONJ
aiua-14101	3	21	somatic	somatic	ADJ
aiua-14101	3	22	cells	cell	NOUN
aiua-14101	3	23	.	.	PUNCT
aiua-14101	4	1	the	the	DET
aiua-14101	4	2	successful	successful	ADJ
aiua-14101	4	3	cryopreservation	cryopreservation	NOUN
aiua-14101	4	4	of	of	ADP
aiua-14101	4	5	rat	rat	NOUN
aiua-14101	4	6	spermatozoa	spermatozoon	NOUN
aiua-14101	4	7	and	and	CCONJ
aiua-14101	4	8	the	the	DET
aiua-14101	4	9	development	development	NOUN
aiua-14101	4	10	of	of	ADP
aiua-14101	4	11	new	new	ADJ
aiua-14101	4	12	diluents	diluent	NOUN
aiua-14101	4	13	are	be	AUX
aiua-14101	4	14	important	important	ADJ
aiua-14101	4	15	in	in	ADP
aiua-14101	4	16	biotechnology	biotechnology	NOUN
aiua-14101	4	17	.	.	PUNCT
aiua-14101	5	1	however	however	ADV
aiua-14101	5	2	,	,	PUNCT
aiua-14101	5	3	compared	compare	VERB
aiua-14101	5	4	to	to	ADP
aiua-14101	5	5	many	many	ADJ
aiua-14101	5	6	species	specie	NOUN
aiua-14101	5	7	,	,	PUNCT
aiua-14101	5	8	sperm	sperm	NOUN
aiua-14101	5	9	cryopreservation	cryopreservation	NOUN
aiua-14101	5	10	protocols	protocol	NOUN
aiua-14101	5	11	for	for	ADP
aiua-14101	5	12	preserving	preserve	VERB
aiua-14101	5	13	the	the	DET
aiua-14101	5	14	rat	rat	NOUN
aiua-14101	5	15	strain	strain	NOUN
aiua-14101	5	16	and	and	CCONJ
aiua-14101	5	17	protocols	protocol	NOUN
aiua-14101	5	18	for	for	ADP
aiua-14101	5	19	in	in	ADP
aiua-14101	5	20	vitro	vitro	X
aiua-14101	5	21	fertilization	fertilization	NOUN
aiua-14101	5	22	of	of	ADP
aiua-14101	5	23	oocytes	oocyte	NOUN
aiua-14101	5	24	with	with	ADP
aiua-14101	5	25	frozen	frozen	ADJ
aiua-14101	5	26	sperm	sperm	NOUN
aiua-14101	5	27	remain	remain	VERB
aiua-14101	5	28	under	under	ADP
aiua-14101	5	29	development	development	NOUN
aiua-14101	5	30	.	.	PUNCT
aiua-14101	6	1	although	although	SCONJ
aiua-14101	6	2	many	many	ADJ
aiua-14101	6	3	relevant	relevant	ADJ
aiua-14101	6	4	studies	study	NOUN
aiua-14101	6	5	have	have	AUX
aiua-14101	6	6	been	be	AUX
aiua-14101	6	7	conducted	conduct	VERB
aiua-14101	6	8	(	(	PUNCT
aiua-14101	6	9	1	1	NUM
aiua-14101	6	10	-	-	SYM
aiua-14101	6	11	4	4	NUM
aiua-14101	6	12	)	)	PUNCT
aiua-14101	6	13	,	,	PUNCT
aiua-14101	6	14	the	the	DET
aiua-14101	6	15	ultimate	ultimate	ADJ
aiua-14101	6	16	objective	objective	NOUN
aiua-14101	6	17	remains	remain	VERB
aiua-14101	6	18	unachieved	unachieved	ADJ
aiua-14101	6	19	.	.	PUNCT
aiua-14101	7	1	therefore	therefore	ADV
aiua-14101	7	2	,	,	PUNCT
aiua-14101	7	3	successful	successful	ADJ
aiua-14101	7	4	cryopreservation	cryopreservation	NOUN
aiua-14101	7	5	of	of	ADP
aiua-14101	7	6	rat	rat	NOUN
aiua-14101	7	7	sperms	sperm	NOUN
aiua-14101	7	8	would	would	AUX
aiua-14101	7	9	provide	provide	VERB
aiua-14101	7	10	an	an	DET
aiua-14101	7	11	important	important	ADJ
aiua-14101	7	12	resource	resource	NOUN
aiua-14101	7	13	to	to	PART
aiua-14101	7	14	preserve	preserve	VERB
aiua-14101	7	15	and	and	CCONJ
aiua-14101	7	16	increase	increase	VERB
aiua-14101	7	17	the	the	DET
aiua-14101	7	18	number	number	NOUN
aiua-14101	7	19	of	of	ADP
aiua-14101	7	20	genetically	genetically	ADV
aiua-14101	7	21	valuable	valuable	ADJ
aiua-14101	7	22	strains	strain	NOUN
aiua-14101	7	23	for	for	ADP
aiua-14101	7	24	research	research	NOUN
aiua-14101	7	25	and	and	CCONJ
aiua-14101	7	26	applications	application	NOUN
aiua-14101	7	27	.	.	PUNCT
aiua-14101	8	1	factors	factor	NOUN
aiua-14101	8	2	affecting	affect	VERB
aiua-14101	8	3	the	the	DET
aiua-14101	8	4	success	success	NOUN
aiua-14101	8	5	of	of	ADP
aiua-14101	8	6	sperm	sperm	NOUN
aiua-14101	8	7	cryopreservation	cryopreservation	NOUN
aiua-14101	8	8	are	be	AUX
aiua-14101	8	9	that	that	SCONJ
aiua-14101	8	10	the	the	DET
aiua-14101	8	11	head	head	NOUN
aiua-14101	8	12	region	region	NOUN
aiua-14101	8	13	of	of	ADP
aiua-14101	8	14	rat	rat	NOUN
aiua-14101	8	15	spermatozoa	spermatozoon	NOUN
aiua-14101	8	16	is	be	AUX
aiua-14101	8	17	shaped	shape	VERB
aiua-14101	8	18	in	in	ADP
aiua-14101	8	19	a	a	DET
aiua-14101	8	20	different	different	ADJ
aiua-14101	8	21	structure	structure	NOUN
aiua-14101	8	22	than	than	ADP
aiua-14101	8	23	that	that	PRON
aiua-14101	8	24	of	of	ADP
aiua-14101	8	25	mammals	mammal	NOUN
aiua-14101	8	26	,	,	PUNCT
aiua-14101	8	27	the	the	DET
aiua-14101	8	28	tail	tail	NOUN
aiua-14101	8	29	structure	structure	NOUN
aiua-14101	8	30	is	be	AUX
aiua-14101	8	31	longer	long	ADJ
aiua-14101	8	32	than	than	ADP
aiua-14101	8	33	that	that	PRON
aiua-14101	8	34	of	of	ADP
aiua-14101	8	35	other	other	ADJ
aiua-14101	8	36	animal	animal	NOUN
aiua-14101	8	37	species	specie	NOUN
aiua-14101	8	38	,	,	PUNCT
aiua-14101	8	39	and	and	CCONJ
aiua-14101	8	40	the	the	DET
aiua-14101	8	41	water	water	NOUN
aiua-14101	8	42	permeability	permeability	NOUN
aiua-14101	8	43	of	of	ADP
aiua-14101	8	44	the	the	DET
aiua-14101	8	45	plasma	plasma	NOUN
aiua-14101	8	46	membrane	membrane	NOUN
aiua-14101	8	47	is	be	AUX
aiua-14101	8	48	low	low	ADJ
aiua-14101	8	49	(	(	PUNCT
aiua-14101	8	50	3	3	NUM
aiua-14101	8	51	,	,	PUNCT
aiua-14101	8	52	5	5	NUM
aiua-14101	8	53	,	,	PUNCT
aiua-14101	8	54	6	6	NUM
aiua-14101	8	55	)	)	PUNCT
aiua-14101	8	56	.	.	PUNCT
aiua-14101	9	1	therefore	therefore	ADV
aiua-14101	9	2	,	,	PUNCT
aiua-14101	9	3	various	various	ADJ
aiua-14101	9	4	antioxidant	antioxidant	ADJ
aiua-14101	9	5	substances	substance	NOUN
aiua-14101	9	6	and	and	CCONJ
aiua-14101	9	7	biomolecules	biomolecule	NOUN
aiua-14101	9	8	are	be	AUX
aiua-14101	9	9	supplemented	supplement	VERB
aiua-14101	9	10	to	to	ADP
aiua-14101	9	11	cryopreservation	cryopreservation	NOUN
aiua-14101	9	12	media	medium	NOUN
aiua-14101	9	13	for	for	ADP
aiua-14101	9	14	healthy	healthy	ADJ
aiua-14101	9	15	storage	storage	NOUN
aiua-14101	9	16	of	of	ADP
aiua-14101	9	17	rat	rat	NOUN
aiua-14101	9	18	sperm	sperm	NOUN
aiua-14101	9	19	.	.	PUNCT
aiua-14101	10	1	in	in	ADP
aiua-14101	10	2	this	this	DET
aiua-14101	10	3	study	study	NOUN
aiua-14101	10	4	,	,	PUNCT
aiua-14101	10	5	tryptophan	tryptophan	NOUN
aiua-14101	10	6	,	,	PUNCT
aiua-14101	10	7	an	an	DET
aiua-14101	10	8	aromatic	aromatic	ADJ
aiua-14101	10	9	amino	amino	NOUN
aiua-14101	10	10	acid	acid	NOUN
aiua-14101	10	11	,	,	PUNCT
aiua-14101	10	12	and	and	CCONJ
aiua-14101	10	13	trehalose	trehalose	ADV
aiua-14101	10	14	,	,	PUNCT
aiua-14101	10	15	a	a	DET
aiua-14101	10	16	disaccharide	disaccharide	NOUN
aiua-14101	10	17	,	,	PUNCT
aiua-14101	10	18	were	be	AUX
aiua-14101	10	19	supplemented	supplement	VERB
aiua-14101	10	20	to	to	ADP
aiua-14101	10	21	the	the	DET
aiua-14101	10	22	cryopreservation	cryopreservation	NOUN
aiua-14101	10	23	diluent	diluent	NOUN
aiua-14101	10	24	to	to	PART
aiua-14101	10	25	increase	increase	VERB
aiua-14101	10	26	cryosurvival	cryosurvival	NOUN
aiua-14101	10	27	.	.	PUNCT
aiua-14101	11	1	amino	amino	ADJ
aiua-14101	11	2	acids	acid	NOUN
aiua-14101	11	3	are	be	AUX
aiua-14101	11	4	the	the	DET
aiua-14101	11	5	building	building	NOUN
aiua-14101	11	6	blocks	block	NOUN
aiua-14101	11	7	of	of	ADP
aiua-14101	11	8	proteins	protein	NOUN
aiua-14101	11	9	and	and	CCONJ
aiua-14101	11	10	have	have	VERB
aiua-14101	11	11	important	important	ADJ
aiua-14101	11	12	roles	role	NOUN
aiua-14101	11	13	in	in	ADP
aiua-14101	11	14	various	various	ADJ
aiua-14101	11	15	biological	biological	ADJ
aiua-14101	11	16	and	and	CCONJ
aiua-14101	11	17	physiological	physiological	ADJ
aiua-14101	11	18	processes	process	NOUN
aiua-14101	11	19	(	(	PUNCT
aiua-14101	11	20	7	7	NUM
aiua-14101	11	21	)	)	PUNCT
aiua-14101	11	22	.	.	PUNCT
aiua-14101	12	1	their	their	PRON
aiua-14101	12	2	antioxidant	antioxidant	ADJ
aiua-14101	12	3	properties	property	NOUN
aiua-14101	12	4	provide	provide	VERB
aiua-14101	12	5	effective	effective	ADJ
aiua-14101	12	6	primary	primary	ADJ
aiua-14101	12	7	defense	defense	NOUN
aiua-14101	12	8	for	for	ADP
aiua-14101	12	9	the	the	DET
aiua-14101	12	10	protection	protection	NOUN
aiua-14101	12	11	of	of	ADP
aiua-14101	12	12	sperm	sperm	NOUN
aiua-14101	12	13	plasmalemma	plasmalemma	PROPN
aiua-14101	12	14	(	(	PUNCT
aiua-14101	12	15	8)	8)	NUM
aiua-14101	12	16	.	.	PUNCT
aiua-14101	12	17	tryptophan	tryptophan	NOUN
aiua-14101	12	18	,	,	PUNCT
aiua-14101	12	19	an	an	DET
aiua-14101	12	20	essential	essential	ADJ
aiua-14101	12	21	aromatic	aromatic	ADJ
aiua-14101	12	22	amino	amino	NOUN
aiua-14101	12	23	acid	acid	NOUN
aiua-14101	12	24	,	,	PUNCT
aiua-14101	12	25	is	be	AUX
aiua-14101	12	26	the	the	DET
aiua-14101	12	27	precursor	precursor	NOUN
aiua-14101	12	28	of	of	ADP
aiua-14101	12	29	many	many	ADJ
aiua-14101	12	30	active	active	ADJ
aiua-14101	12	31	molecules	molecule	NOUN
aiua-14101	12	32	including	include	VERB
aiua-14101	12	33	serotonin	serotonin	NOUN
aiua-14101	12	34	,	,	PUNCT
aiua-14101	12	35	melatonin	melatonin	NOUN
aiua-14101	12	36	,	,	PUNCT
aiua-14101	12	37	and	and	CCONJ
aiua-14101	12	38	kynurenic	kynurenic	ADJ
aiua-14101	12	39	acid	acid	NOUN
aiua-14101	12	40	,	,	PUNCT
aiua-14101	12	41	which	which	PRON
aiua-14101	12	42	are	be	AUX
aiua-14101	12	43	antioxidants	antioxidant	NOUN
aiua-14101	12	44	against	against	ADP
aiua-14101	12	45	free	free	ADJ
aiua-14101	12	46	radicals	radical	NOUN
aiua-14101	12	47	causing	cause	VERB
aiua-14101	12	48	oxidative	oxidative	ADJ
aiua-14101	12	49	stress	stress	NOUN
aiua-14101	12	50	in	in	ADP
aiua-14101	12	51	spermatozoa	spermatozoa	NOUN
aiua-14101	12	52	(	(	PUNCT
aiua-14101	12	53	9	9	NUM
aiua-14101	12	54	,	,	PUNCT
aiua-14101	12	55	10	10	NUM
aiua-14101	12	56	)	)	PUNCT
aiua-14101	12	57	.	.	PUNCT
aiua-14101	13	1	it	it	PRON
aiua-14101	13	2	is	be	AUX
aiua-14101	13	3	widely	widely	ADV
aiua-14101	13	4	used	use	VERB
aiua-14101	13	5	in	in	ADP
aiua-14101	13	6	numerous	numerous	ADJ
aiua-14101	13	7	research	research	NOUN
aiua-14101	13	8	and	and	CCONJ
aiua-14101	13	9	clinical	clinical	ADJ
aiua-14101	13	10	studies	study	NOUN
aiua-14101	13	11	(	(	PUNCT
aiua-14101	13	12	9	9	NUM
aiua-14101	13	13	-	-	SYM
aiua-14101	13	14	11	11	NUM
aiua-14101	13	15	)	)	PUNCT
aiua-14101	13	16	and	and	CCONJ
aiua-14101	13	17	is	be	AUX
aiua-14101	13	18	therefore	therefore	ADV
aiua-14101	13	19	anticipated	anticipate	VERB
aiua-14101	13	20	as	as	ADP
aiua-14101	13	21	an	an	DET
aiua-14101	13	22	additive	additive	NOUN
aiua-14101	13	23	in	in	ADP
aiua-14101	13	24	cryopreservation	cryopreservation	NOUN
aiua-14101	13	25	protocols	protocol	NOUN
aiua-14101	13	26	.	.	PUNCT
aiua-14101	14	1	sugars	sugar	NOUN
aiua-14101	14	2	are	be	AUX
aiua-14101	14	3	large	large	ADJ
aiua-14101	14	4	molecules	molecule	NOUN
aiua-14101	14	5	that	that	PRON
aiua-14101	14	6	interact	interact	VERB
aiua-14101	14	7	with	with	ADP
aiua-14101	14	8	the	the	DET
aiua-14101	14	9	plasma	plasma	NOUN
aiua-14101	14	10	membrane	membrane	NOUN
aiua-14101	14	11	and	and	CCONJ
aiua-14101	14	12	energize	energize	VERB
aiua-14101	14	13	the	the	DET
aiua-14101	14	14	cell	cell	NOUN
aiua-14101	14	15	.	.	PUNCT
aiua-14101	15	1	they	they	PRON
aiua-14101	15	2	are	be	AUX
aiua-14101	15	3	widely	widely	ADV
aiua-14101	15	4	used	use	VERB
aiua-14101	15	5	in	in	ADP
aiua-14101	15	6	cryopreservation	cryopreservation	NOUN
aiua-14101	15	7	solutions	solution	NOUN
aiua-14101	15	8	as	as	SCONJ
aiua-14101	15	9	they	they	PRON
aiua-14101	15	10	stabilize	stabilize	VERB
aiua-14101	15	11	intracellular	intracellular	ADJ
aiua-14101	15	12	and	and	CCONJ
aiua-14101	15	13	extracellular	extracellular	ADJ
aiua-14101	15	14	osmotic	osmotic	ADJ
aiua-14101	15	15	pressure	pressure	NOUN
aiua-14101	15	16	(	(	PUNCT
aiua-14101	15	17	12	12	NUM
aiua-14101	15	18	)	)	PUNCT
aiua-14101	15	19	.	.	PUNCT
aiua-14101	16	1	trehalose	trehalose	PROPN
aiua-14101	16	2	is	be	AUX
aiua-14101	16	3	a	a	DET
aiua-14101	16	4	background	background	NOUN
aiua-14101	16	5	:	:	PUNCT
aiua-14101	16	6	cryopreservation	cryopreservation	NOUN
aiua-14101	16	7	of	of	ADP
aiua-14101	16	8	rat	rat	NOUN
aiua-14101	16	9	sperm	sperm	NOUN
aiua-14101	16	10	cells	cell	NOUN
aiua-14101	16	11	and	and	CCONJ
aiua-14101	16	12	development	development	NOUN
aiua-14101	16	13	of	of	ADP
aiua-14101	16	14	new	new	ADJ
aiua-14101	16	15	diluents	diluent	NOUN
aiua-14101	16	16	are	be	AUX
aiua-14101	16	17	very	very	ADV
aiua-14101	16	18	important	important	ADJ
aiua-14101	16	19	in	in	ADP
aiua-14101	16	20	biotechnology	biotechnology	NOUN
aiua-14101	16	21	.	.	PUNCT
aiua-14101	17	1	methods	method	NOUN
aiua-14101	17	2	:	:	PUNCT
aiua-14101	17	3	in	in	ADP
aiua-14101	17	4	the	the	DET
aiua-14101	17	5	current	current	ADJ
aiua-14101	17	6	study	study	NOUN
aiua-14101	17	7	,	,	PUNCT
aiua-14101	17	8	25	25	NUM
aiua-14101	17	9	mm	mm	NOUN
aiua-14101	17	10	tryptophan	tryptophan	NOUN
aiua-14101	17	11	and	and	CCONJ
aiua-14101	17	12	100	100	NUM
aiua-14101	17	13	mm	mm	NOUN
aiua-14101	17	14	trehalose	trehalose	INTJ
aiua-14101	17	15	were	be	AUX
aiua-14101	17	16	added	add	VERB
aiua-14101	17	17	separately	separately	ADV
aiua-14101	17	18	to	to	ADP
aiua-14101	17	19	the	the	DET
aiua-14101	17	20	cryopreservation	cryopreservation	NOUN
aiua-14101	17	21	medium	medium	NOUN
aiua-14101	17	22	containing	contain	VERB
aiua-14101	17	23	8	8	NUM
aiua-14101	17	24	%	%	NOUN
aiua-14101	17	25	lactose	lactose	NOUN
aiua-14101	17	26	monohydrate	monohydrate	NOUN
aiua-14101	17	27	,	,	PUNCT
aiua-14101	17	28	23	23	NUM
aiua-14101	17	29	%	%	NOUN
aiua-14101	17	30	egg	egg	NOUN
aiua-14101	17	31	yolk	yolk	NOUN
aiua-14101	17	32	,	,	PUNCT
aiua-14101	17	33	and	and	CCONJ
aiua-14101	17	34	10	10	NUM
aiua-14101	17	35	%	%	NOUN
aiua-14101	17	36	tris	tris	NOUN
aiua-14101	17	37	aminomethane	aminomethane	ADJ
aiua-14101	17	38	to	to	PART
aiua-14101	17	39	evaluate	evaluate	VERB
aiua-14101	17	40	the	the	DET
aiua-14101	17	41	cryopreservation	cryopreservation	NOUN
aiua-14101	17	42	capability	capability	NOUN
aiua-14101	17	43	of	of	ADP
aiua-14101	17	44	rat	rat	NOUN
aiua-14101	17	45	sperms	sperm	NOUN
aiua-14101	17	46	.	.	PUNCT
aiua-14101	18	1	after	after	SCONJ
aiua-14101	18	2	freeze	freeze	NOUN
aiua-14101	18	3	-	-	PUNCT
aiua-14101	18	4	thawing	thaw	VERB
aiua-14101	18	5	,	,	PUNCT
aiua-14101	18	6	motility	motility	NOUN
aiua-14101	18	7	,	,	PUNCT
aiua-14101	18	8	viable	viable	ADJ
aiua-14101	18	9	spermatozoon	spermatozoon	NOUN
aiua-14101	18	10	ratio	ratio	NOUN
aiua-14101	18	11	,	,	PUNCT
aiua-14101	18	12	plasma	plasma	NOUN
aiua-14101	18	13	membrane	membrane	NOUN
aiua-14101	18	14	integrity	integrity	NOUN
aiua-14101	18	15	,	,	PUNCT
aiua-14101	18	16	abnormal	abnormal	ADJ
aiua-14101	18	17	acrosome	acrosome	NOUN
aiua-14101	18	18	ratio	ratio	NOUN
aiua-14101	18	19	,	,	PUNCT
aiua-14101	18	20	and	and	CCONJ
aiua-14101	18	21	apoptotic	apoptotic	ADJ
aiua-14101	18	22	cell	cell	NOUN
aiua-14101	18	23	ratio	ratio	NOUN
aiua-14101	18	24	and	and	CCONJ
aiua-14101	18	25	levels	level	NOUN
aiua-14101	18	26	of	of	ADP
aiua-14101	18	27	bax	bax	PROPN
aiua-14101	18	28	,	,	PUNCT
aiua-14101	18	29	bcl-2	bcl-2	NOUN
aiua-14101	18	30	,	,	PUNCT
aiua-14101	18	31	and	and	CCONJ
aiua-14101	18	32	caspase-3	caspase-3	NUM
aiua-14101	18	33	mrna	mrna	NOUN
aiua-14101	18	34	involved	involve	VERB
aiua-14101	18	35	in	in	ADP
aiua-14101	18	36	the	the	DET
aiua-14101	18	37	apoptosis	apoptosis	NOUN
aiua-14101	18	38	pathway	pathway	NOUN
aiua-14101	18	39	were	be	AUX
aiua-14101	18	40	evaluated	evaluate	VERB
aiua-14101	18	41	.	.	PUNCT
aiua-14101	19	1	results	result	NOUN
aiua-14101	19	2	:	:	PUNCT
aiua-14101	19	3	the	the	DET
aiua-14101	19	4	highest	high	ADJ
aiua-14101	19	5	viability	viability	NOUN
aiua-14101	19	6	ratio	ratio	NOUN
aiua-14101	19	7	was	be	AUX
aiua-14101	19	8	detected	detect	VERB
aiua-14101	19	9	in	in	ADP
aiua-14101	19	10	the	the	DET
aiua-14101	19	11	trehalose	trehalose	PROPN
aiua-14101	19	12	group	group	NOUN
aiua-14101	19	13	(	(	PUNCT
aiua-14101	19	14	26.25	26.25	NUM
aiua-14101	19	15	±	±	NUM
aiua-14101	19	16	3.10	3.10	NUM
aiua-14101	19	17	)	)	PUNCT
aiua-14101	19	18	,	,	PUNCT
aiua-14101	19	19	which	which	PRON
aiua-14101	19	20	was	be	AUX
aiua-14101	19	21	significantly	significantly	ADV
aiua-14101	19	22	higher	high	ADJ
aiua-14101	19	23	than	than	ADP
aiua-14101	19	24	that	that	PRON
aiua-14101	19	25	in	in	ADP
aiua-14101	19	26	the	the	DET
aiua-14101	19	27	control	control	NOUN
aiua-14101	19	28	group	group	NOUN
aiua-14101	19	29	(	(	PUNCT
aiua-14101	19	30	19.62	19.62	NUM
aiua-14101	19	31	±	±	NUM
aiua-14101	19	32	1.51	1.51	NUM
aiua-14101	19	33	)	)	PUNCT
aiua-14101	19	34	(	(	PUNCT
aiua-14101	19	35	p	p	X
aiua-14101	19	36	<	<	X
aiua-14101	19	37	0.05	0.05	NUM
aiua-14101	19	38	)	)	PUNCT
aiua-14101	19	39	.	.	PUNCT
aiua-14101	20	1	when	when	SCONJ
aiua-14101	20	2	compared	compare	VERB
aiua-14101	20	3	to	to	ADP
aiua-14101	20	4	the	the	DET
aiua-14101	20	5	tryptophan	tryptophan	NOUN
aiua-14101	20	6	group	group	NOUN
aiua-14101	20	7	(	(	PUNCT
aiua-14101	20	8	11.87	11.87	NUM
aiua-14101	20	9	±	±	NUM
aiua-14101	20	10	3.07	3.07	NUM
aiua-14101	20	11	)	)	PUNCT
aiua-14101	20	12	,	,	PUNCT
aiua-14101	20	13	plasma	plasma	NOUN
aiua-14101	20	14	membrane	membrane	NOUN
aiua-14101	20	15	integrity	integrity	NOUN
aiua-14101	20	16	was	be	AUX
aiua-14101	20	17	statistically	statistically	ADV
aiua-14101	20	18	higher	high	ADJ
aiua-14101	20	19	in	in	ADP
aiua-14101	20	20	the	the	DET
aiua-14101	20	21	trehalose	trehalose	ADJ
aiua-14101	20	22	group	group	NOUN
aiua-14101	20	23	(	(	PUNCT
aiua-14101	20	24	20.25	20.25	NUM
aiua-14101	20	25	±	±	NUM
aiua-14101	20	26	3.65	3.65	NUM
aiua-14101	20	27	)	)	PUNCT
aiua-14101	20	28	(	(	PUNCT
aiua-14101	20	29	p	p	X
aiua-14101	20	30	<	<	X
aiua-14101	20	31	0.05	0.05	NUM
aiua-14101	20	32	)	)	PUNCT
aiua-14101	20	33	.	.	PUNCT
aiua-14101	21	1	the	the	DET
aiua-14101	21	2	rate	rate	NOUN
aiua-14101	21	3	of	of	ADP
aiua-14101	21	4	apoptotic	apoptotic	ADJ
aiua-14101	21	5	cells	cell	NOUN
aiua-14101	21	6	was	be	AUX
aiua-14101	21	7	lower	low	ADJ
aiua-14101	21	8	in	in	ADP
aiua-14101	21	9	the	the	DET
aiua-14101	21	10	trehalose	trehalose	ADJ
aiua-14101	21	11	group	group	NOUN
aiua-14101	21	12	than	than	ADP
aiua-14101	21	13	in	in	ADP
aiua-14101	21	14	the	the	DET
aiua-14101	21	15	control	control	NOUN
aiua-14101	21	16	group	group	NOUN
aiua-14101	21	17	.	.	PUNCT
aiua-14101	22	1	then	then	ADV
aiua-14101	22	2	,	,	PUNCT
aiua-14101	22	3	the	the	DET
aiua-14101	22	4	effect	effect	NOUN
aiua-14101	22	5	of	of	ADP
aiua-14101	22	6	tryptophan	tryptophan	NOUN
aiua-14101	22	7	and	and	CCONJ
aiua-14101	22	8	trehalose	trehalose	NOUN
aiua-14101	22	9	,	,	PUNCT
aiua-14101	22	10	which	which	PRON
aiua-14101	22	11	were	be	AUX
aiua-14101	22	12	added	add	VERB
aiua-14101	22	13	to	to	ADP
aiua-14101	22	14	the	the	DET
aiua-14101	22	15	sperm	sperm	NOUN
aiua-14101	22	16	diluent	diluent	NOUN
aiua-14101	22	17	,	,	PUNCT
aiua-14101	22	18	on	on	ADP
aiua-14101	22	19	the	the	DET
aiua-14101	22	20	expression	expression	NOUN
aiua-14101	22	21	levels	level	NOUN
aiua-14101	22	22	of	of	ADP
aiua-14101	22	23	genes	gene	NOUN
aiua-14101	22	24	in	in	ADP
aiua-14101	22	25	the	the	DET
aiua-14101	22	26	apoptosis	apoptosis	NOUN
aiua-14101	22	27	pathway	pathway	NOUN
aiua-14101	22	28	was	be	AUX
aiua-14101	22	29	evaluated	evaluate	VERB
aiua-14101	22	30	through	through	ADP
aiua-14101	22	31	the	the	DET
aiua-14101	22	32	rt	rt	NOUN
aiua-14101	22	33	-	-	PUNCT
aiua-14101	22	34	qpcr	qpcr	ADJ
aiua-14101	22	35	technique	technique	NOUN
aiua-14101	22	36	.	.	PUNCT
aiua-14101	23	1	the	the	DET
aiua-14101	23	2	expression	expression	NOUN
aiua-14101	23	3	of	of	ADP
aiua-14101	23	4	the	the	DET
aiua-14101	23	5	anti	anti	ADJ
aiua-14101	23	6	-	-	ADJ
aiua-14101	23	7	apoptotic	apoptotic	ADJ
aiua-14101	23	8	gene	gene	NOUN
aiua-14101	23	9	bcl-2	bcl-2	VERB
aiua-14101	23	10	increased	increase	VERB
aiua-14101	23	11	by	by	ADP
aiua-14101	23	12	25	25	NUM
aiua-14101	23	13	mm	mm	NOUN
aiua-14101	23	14	tryptophan	tryptophan	NOUN
aiua-14101	23	15	and	and	CCONJ
aiua-14101	23	16	100	100	NUM
aiua-14101	23	17	mm	mm	NOUN
aiua-14101	23	18	trehalose	trehalose	INTJ
aiua-14101	23	19	,	,	PUNCT
aiua-14101	23	20	while	while	SCONJ
aiua-14101	23	21	the	the	DET
aiua-14101	23	22	expression	expression	NOUN
aiua-14101	23	23	of	of	ADP
aiua-14101	23	24	the	the	DET
aiua-14101	23	25	pro	pro	ADJ
aiua-14101	23	26	-	-	ADJ
aiua-14101	23	27	apoptotic	apoptotic	ADJ
aiua-14101	23	28	genes	gene	NOUN
aiua-14101	23	29	bax	bax	PROPN
aiua-14101	23	30	and	and	CCONJ
aiua-14101	23	31	caspase-3	caspase-3	NUM
aiua-14101	23	32	decreased	decrease	VERB
aiua-14101	23	33	(	(	PUNCT
aiua-14101	23	34	p	p	X
aiua-14101	23	35	<	<	X
aiua-14101	23	36	0.01	0.01	NUM
aiua-14101	23	37	)	)	PUNCT
aiua-14101	23	38	.	.	PUNCT
aiua-14101	24	1	conclusions	conclusion	NOUN
aiua-14101	24	2	:	:	PUNCT
aiua-14101	24	3	as	as	ADP
aiua-14101	24	4	a	a	DET
aiua-14101	24	5	result	result	NOUN
aiua-14101	24	6	,	,	PUNCT
aiua-14101	24	7	it	it	PRON
aiua-14101	24	8	was	be	AUX
aiua-14101	24	9	determined	determine	VERB
aiua-14101	24	10	that	that	SCONJ
aiua-14101	24	11	trehalose	trehalose	ADV
aiua-14101	24	12	,	,	PUNCT
aiua-14101	24	13	a	a	DET
aiua-14101	24	14	isaccharide	isaccharide	NOUN
aiua-14101	24	15	sugar	sugar	NOUN
aiua-14101	24	16	,	,	PUNCT
aiua-14101	24	17	added	add	VERB
aiua-14101	24	18	to	to	ADP
aiua-14101	24	19	the	the	DET
aiua-14101	24	20	rat	rat	NOUN
aiua-14101	24	21	sperm	sperm	NOUN
aiua-14101	24	22	diluent	diluent	NOUN
aiua-14101	24	23	provided	provide	VERB
aiua-14101	24	24	more	more	ADV
aiua-14101	24	25	effective	effective	ADJ
aiua-14101	24	26	protection	protection	NOUN
aiua-14101	24	27	against	against	ADP
aiua-14101	24	28	cryodamage	cryodamage	NOUN
aiua-14101	24	29	in	in	ADP
aiua-14101	24	30	the	the	DET
aiua-14101	24	31	cryopreservation	cryopreservation	NOUN
aiua-14101	24	32	process	process	NOUN
aiua-14101	24	33	than	than	ADP
aiua-14101	24	34	tryptophan	tryptophan	NOUN
aiua-14101	24	35	,	,	PUNCT
aiua-14101	24	36	an	an	DET
aiua-14101	24	37	aromatic	aromatic	ADJ
aiua-14101	24	38	amino	amino	NOUN
aiua-14101	24	39	acid	acid	NOUN
aiua-14101	24	40	,	,	PUNCT
aiua-14101	24	41	according	accord	VERB
aiua-14101	24	42	to	to	ADP
aiua-14101	24	43	the	the	DET
aiua-14101	24	44	parameters	parameter	NOUN
aiua-14101	24	45	evaluated	evaluate	VERB
aiua-14101	24	46	.	.	PUNCT
aiua-14101	25	1	from	from	ADP
aiua-14101	25	2	a	a	DET
aiua-14101	25	3	molecular	molecular	ADJ
aiua-14101	25	4	biotechnology	biotechnology	NOUN
aiua-14101	25	5	perspective	perspective	NOUN
aiua-14101	25	6	,	,	PUNCT
aiua-14101	25	7	these	these	DET
aiua-14101	25	8	findings	finding	NOUN
aiua-14101	25	9	lay	lie	VERB
aiua-14101	25	10	a	a	DET
aiua-14101	25	11	valuable	valuable	ADJ
aiua-14101	25	12	foundation	foundation	NOUN
aiua-14101	25	13	for	for	ADP
aiua-14101	25	14	research	research	NOUN
aiua-14101	25	15	on	on	ADP
aiua-14101	25	16	the	the	DET
aiua-14101	25	17	development	development	NOUN
aiua-14101	25	18	of	of	ADP
aiua-14101	25	19	next	next	ADJ
aiua-14101	25	20	generation	generation	NOUN
aiua-14101	25	21	media	medium	NOUN
aiua-14101	25	22	for	for	ADP
aiua-14101	25	23	sperm	sperm	NOUN
aiua-14101	25	24	cryopreservation	cryopreservation	NOUN
aiua-14101	25	25	.	.	PUNCT
aiua-14101	26	1	key	key	ADJ
aiua-14101	26	2	words	word	NOUN
aiua-14101	26	3	:	:	PUNCT
aiua-14101	26	4	cryopreservation	cryopreservation	NOUN
aiua-14101	26	5	;	;	PUNCT
aiua-14101	26	6	rat	rat	NOUN
aiua-14101	26	7	;	;	PUNCT
aiua-14101	26	8	tryptophan	tryptophan	NOUN
aiua-14101	26	9	;	;	PUNCT
aiua-14101	26	10	trehalose	trehalose	ADV
aiua-14101	26	11	;	;	PUNCT
aiua-14101	26	12	gene	gene	NOUN
aiua-14101	26	13	expression	expression	NOUN
aiua-14101	26	14	.	.	PUNCT
aiua-14101	26	15	submitted	submit	VERB
aiua-14101	26	16	27	27	NUM
aiua-14101	26	17	june	june	PROPN
aiua-14101	26	18	2025	2025	NUM
aiua-14101	26	19	;	;	PUNCT
aiua-14101	26	20	accepted	accept	VERB
aiua-14101	26	21	27	27	NUM
aiua-14101	26	22	july	july	PROPN
aiua-14101	26	23	2025	2025	NUM
aiua-14101	26	24	introduction	introduction	NOUN
aiua-14101	26	25	in	in	ADP
aiua-14101	26	26	molecular	molecular	ADJ
aiua-14101	26	27	biology	biology	NOUN
aiua-14101	26	28	and	and	CCONJ
aiua-14101	26	29	genetics	genetic	NOUN
aiua-14101	26	30	,	,	PUNCT
aiua-14101	26	31	rats	rat	NOUN
aiua-14101	26	32	are	be	AUX
aiua-14101	26	33	used	use	VERB
aiua-14101	26	34	as	as	ADP
aiua-14101	26	35	an	an	DET
aiua-14101	26	36	alternative	alternative	NOUN
aiua-14101	26	37	for	for	ADP
aiua-14101	26	38	creating	create	VERB
aiua-14101	26	39	many	many	ADJ
aiua-14101	26	40	disease	disease	NOUN
aiua-14101	26	41	models	model	NOUN
aiua-14101	26	42	.	.	PUNCT
aiua-14101	27	1	therefore	therefore	ADV
aiua-14101	27	2	,	,	PUNCT
aiua-14101	27	3	the	the	DET
aiua-14101	27	4	use	use	NOUN
aiua-14101	27	5	of	of	ADP
aiua-14101	27	6	rats	rat	NOUN
aiua-14101	27	7	as	as	ADP
aiua-14101	27	8	experimental	experimental	ADJ
aiua-14101	27	9	animals	animal	NOUN
aiua-14101	27	10	has	have	AUX
aiua-14101	27	11	been	be	AUX
aiua-14101	27	12	expandimpact	expandimpact	NOUN
aiua-14101	27	13	of	of	ADP
aiua-14101	27	14	tryptophan	tryptophan	NOUN
aiua-14101	27	15	and	and	CCONJ
aiua-14101	27	16	trehalose	trehalose	NOUN
aiua-14101	27	17	on	on	ADP
aiua-14101	27	18	post	post	ADJ
aiua-14101	27	19	-	-	ADJ
aiua-14101	27	20	thaw	thaw	ADJ
aiua-14101	27	21	sperm	sperm	NOUN
aiua-14101	27	22	quality	quality	NOUN
aiua-14101	27	23	and	and	CCONJ
aiua-14101	27	24	apoptotic	apoptotic	ADJ
aiua-14101	27	25	gene	gene	NOUN
aiua-14101	27	26	expression	expression	NOUN
aiua-14101	27	27	in	in	ADP
aiua-14101	27	28	wistar	wistar	PROPN
aiua-14101	27	29	albino	albino	PROPN
aiua-14101	27	30	rats	rat	NOUN
aiua-14101	27	31	firdevs	firdevs	VERB
aiua-14101	27	32	yilmaz	yilmaz	PROPN
aiua-14101	27	33	dayanc	dayanc	PROPN
aiua-14101	27	34	1	1	NUM
aiua-14101	27	35	,	,	PUNCT
aiua-14101	27	36	oguz	oguz	PROPN
aiua-14101	27	37	kaan	kaan	PROPN
aiua-14101	27	38	yalcin	yalcin	PROPN
aiua-14101	27	39	2	2	NUM
aiua-14101	27	40	,	,	PUNCT
aiua-14101	27	41	aysel	aysel	NOUN
aiua-14101	27	42	eraslan	eraslan	PROPN
aiua-14101	27	43	sakar	sakar	PROPN
aiua-14101	27	44	3	3	NUM
aiua-14101	27	45	1	1	NUM
aiua-14101	27	46	hatay	hatay	NOUN
aiua-14101	27	47	mustafa	mustafa	PROPN
aiua-14101	27	48	kemal	kemal	PROPN
aiua-14101	27	49	university	university	PROPN
aiua-14101	27	50	,	,	PUNCT
aiua-14101	27	51	department	department	NOUN
aiua-14101	27	52	of	of	ADP
aiua-14101	27	53	reproduction	reproduction	NOUN
aiua-14101	27	54	and	and	CCONJ
aiua-14101	27	55	artificial	artificial	ADJ
aiua-14101	27	56	insemination	insemination	NOUN
aiua-14101	27	57	,	,	PUNCT
aiua-14101	27	58	hatay	hatay	NOUN
aiua-14101	27	59	,	,	PUNCT
aiua-14101	27	60	turkey	turkey	NOUN
aiua-14101	27	61	;	;	PUNCT
aiua-14101	27	62	2	2	NUM
aiua-14101	27	63	hatay	hatay	NOUN
aiua-14101	27	64	mustafa	mustafa	PROPN
aiua-14101	27	65	kemal	kemal	PROPN
aiua-14101	27	66	university	university	PROPN
aiua-14101	27	67	,	,	PUNCT
aiua-14101	27	68	faculty	faculty	NOUN
aiua-14101	27	69	of	of	ADP
aiua-14101	27	70	veterinary	veterinary	ADJ
aiua-14101	27	71	medicine	medicine	NOUN
aiua-14101	27	72	,	,	PUNCT
aiua-14101	27	73	department	department	NOUN
aiua-14101	27	74	of	of	ADP
aiua-14101	27	75	reproduction	reproduction	NOUN
aiua-14101	27	76	and	and	CCONJ
aiua-14101	27	77	artificial	artificial	ADJ
aiua-14101	27	78	insemination	insemination	NOUN
aiua-14101	27	79	,	,	PUNCT
aiua-14101	27	80	hatay	hatay	NOUN
aiua-14101	27	81	,	,	PUNCT
aiua-14101	27	82	turkey	turkey	NOUN
aiua-14101	27	83	;	;	PUNCT
aiua-14101	27	84	3	3	NUM
aiua-14101	27	85	hatay	hatay	NOUN
aiua-14101	27	86	mustafa	mustafa	PROPN
aiua-14101	27	87	kemal	kemal	PROPN
aiua-14101	27	88	university	university	PROPN
aiua-14101	27	89	,	,	PUNCT
aiua-14101	27	90	faculty	faculty	NOUN
aiua-14101	27	91	of	of	ADP
aiua-14101	27	92	veterinary	veterinary	ADJ
aiua-14101	27	93	medicine	medicine	NOUN
aiua-14101	27	94	,	,	PUNCT
aiua-14101	27	95	department	department	NOUN
aiua-14101	27	96	of	of	ADP
aiua-14101	27	97	genetics	genetics	PROPN
aiua-14101	27	98	,	,	PUNCT
aiua-14101	27	99	hatay	hatay	NOUN
aiua-14101	27	100	,	,	PUNCT
aiua-14101	27	101	turkey	turkey	NOUN
aiua-14101	27	102	.	.	PUNCT
aiua-14101	28	1	doi	doi	NOUN
aiua-14101	28	2	:	:	PUNCT
aiua-14101	28	3	10.4081	10.4081	NUM
aiua-14101	28	4	/	/	SYM
aiua-14101	28	5	aiua.2025.14101	aiua.2025.14101	PRON
aiua-14101	28	6	summary	summary	VERB
aiua-14101	28	7	archivio	archivio	NOUN
aiua-14101	28	8	italiano	italiano	NOUN
aiua-14101	28	9	di	di	X
aiua-14101	28	10	urologia	urologia	PROPN
aiua-14101	28	11	e	e	PROPN
aiua-14101	28	12	andrologia	andrologia	NOUN
aiua-14101	28	13	2025	2025	NUM
aiua-14101	28	14	;	;	PUNCT
aiua-14101	28	15	97(3):14101	97(3):14101	NUM
aiua-14101	28	16	f.	f.	PROPN
aiua-14101	28	17	yilmaz	yilmaz	PROPN
aiua-14101	28	18	dayanc	dayanc	PROPN
aiua-14101	28	19	,	,	PUNCT
aiua-14101	28	20	o.	o.	PROPN
aiua-14101	28	21	kaan	kaan	PROPN
aiua-14101	28	22	yalcin	yalcin	PROPN
aiua-14101	28	23	,	,	PUNCT
aiua-14101	28	24	a.	a.	NOUN
aiua-14101	28	25	eraslan	eraslan	PROPN
aiua-14101	28	26	sakar	sakar	PROPN
aiua-14101	28	27	2	2	NUM
aiua-14101	28	28	disaccharide	disaccharide	NOUN
aiua-14101	28	29	that	that	PRON
aiua-14101	28	30	alters	alter	VERB
aiua-14101	28	31	membrane	membrane	NOUN
aiua-14101	28	32	fluidity	fluidity	NOUN
aiua-14101	28	33	and	and	CCONJ
aiua-14101	28	34	is	be	AUX
aiua-14101	28	35	present	present	ADJ
aiua-14101	28	36	in	in	ADP
aiua-14101	28	37	the	the	DET
aiua-14101	28	38	phospholipid	phospholipid	NOUN
aiua-14101	28	39	bilayer	bilayer	NOUN
aiua-14101	28	40	of	of	ADP
aiua-14101	28	41	membranes	membrane	NOUN
aiua-14101	28	42	(	(	PUNCT
aiua-14101	28	43	13	13	NUM
aiua-14101	28	44	)	)	PUNCT
aiua-14101	28	45	.	.	PUNCT
aiua-14101	29	1	this	this	DET
aiua-14101	29	2	study	study	NOUN
aiua-14101	29	3	aimed	aim	VERB
aiua-14101	29	4	to	to	PART
aiua-14101	29	5	evaluate	evaluate	VERB
aiua-14101	29	6	the	the	DET
aiua-14101	29	7	effect	effect	NOUN
aiua-14101	29	8	of	of	ADP
aiua-14101	29	9	the	the	DET
aiua-14101	29	10	addition	addition	NOUN
aiua-14101	29	11	of	of	ADP
aiua-14101	29	12	the	the	DET
aiua-14101	29	13	amino	amino	NOUN
aiua-14101	29	14	acid	acid	NOUN
aiua-14101	29	15	tryptophan	tryptophan	NOUN
aiua-14101	29	16	(	(	PUNCT
aiua-14101	29	17	25	25	NUM
aiua-14101	29	18	mm	mm	NOUN
aiua-14101	29	19	)	)	PUNCT
aiua-14101	29	20	and	and	CCONJ
aiua-14101	29	21	disaccharide	disaccharide	VERB
aiua-14101	29	22	trehalose	trehalose	NOUN
aiua-14101	29	23	(	(	PUNCT
aiua-14101	29	24	100	100	NUM
aiua-14101	29	25	mm	mm	NOUN
aiua-14101	29	26	)	)	PUNCT
aiua-14101	29	27	to	to	ADP
aiua-14101	29	28	cryopreservation	cryopreservation	NOUN
aiua-14101	29	29	diluent	diluent	NOUN
aiua-14101	29	30	,	,	PUNCT
aiua-14101	29	31	which	which	PRON
aiua-14101	29	32	have	have	AUX
aiua-14101	29	33	not	not	PART
aiua-14101	29	34	been	be	AUX
aiua-14101	29	35	previously	previously	ADV
aiua-14101	29	36	studied	study	VERB
aiua-14101	29	37	in	in	ADP
aiua-14101	29	38	rats	rat	NOUN
aiua-14101	29	39	,	,	PUNCT
aiua-14101	29	40	on	on	ADP
aiua-14101	29	41	the	the	DET
aiua-14101	29	42	long	long	ADJ
aiua-14101	29	43	-	-	PUNCT
aiua-14101	29	44	term	term	NOUN
aiua-14101	29	45	cryopreservation	cryopreservation	NOUN
aiua-14101	29	46	of	of	ADP
aiua-14101	29	47	rat	rat	NOUN
aiua-14101	29	48	sperm	sperm	NOUN
aiua-14101	29	49	.	.	PUNCT
aiua-14101	30	1	accordingly	accordingly	ADV
aiua-14101	30	2	,	,	PUNCT
aiua-14101	30	3	sperm	sperm	NOUN
aiua-14101	30	4	quality	quality	NOUN
aiua-14101	30	5	parameters	parameter	NOUN
aiua-14101	30	6	such	such	ADJ
aiua-14101	30	7	as	as	ADP
aiua-14101	30	8	motility	motility	NOUN
aiua-14101	30	9	,	,	PUNCT
aiua-14101	30	10	viability	viability	NOUN
aiua-14101	30	11	,	,	PUNCT
aiua-14101	30	12	plasma	plasma	NOUN
aiua-14101	30	13	membrane	membrane	NOUN
aiua-14101	30	14	integrity	integrity	NOUN
aiua-14101	30	15	,	,	PUNCT
aiua-14101	30	16	acrosome	acrosome	NOUN
aiua-14101	30	17	morphology	morphology	NOUN
aiua-14101	30	18	,	,	PUNCT
aiua-14101	30	19	and	and	CCONJ
aiua-14101	30	20	cell	cell	NOUN
aiua-14101	30	21	apoptosis	apoptosis	NOUN
aiua-14101	30	22	ratio	ratio	NOUN
aiua-14101	30	23	,	,	PUNCT
aiua-14101	30	24	and	and	CCONJ
aiua-14101	30	25	expression	expression	NOUN
aiua-14101	30	26	of	of	ADP
aiua-14101	30	27	certain	certain	ADJ
aiua-14101	30	28	genes	gene	NOUN
aiua-14101	30	29	involved	involve	VERB
aiua-14101	30	30	in	in	ADP
aiua-14101	30	31	the	the	DET
aiua-14101	30	32	apoptosis	apoptosis	NOUN
aiua-14101	30	33	pathway	pathway	NOUN
aiua-14101	30	34	(	(	PUNCT
aiua-14101	30	35	bax	bax	PROPN
aiua-14101	30	36	,	,	PUNCT
aiua-14101	30	37	bcl-2	bcl-2	NOUN
aiua-14101	30	38	,	,	PUNCT
aiua-14101	30	39	and	and	CCONJ
aiua-14101	30	40	caspase-3	caspase-3	NUM
aiua-14101	30	41	)	)	PUNCT
aiua-14101	30	42	were	be	AUX
aiua-14101	30	43	examined	examine	VERB
aiua-14101	30	44	to	to	PART
aiua-14101	30	45	develop	develop	VERB
aiua-14101	30	46	new	new	ADJ
aiua-14101	30	47	and	and	CCONJ
aiua-14101	30	48	effective	effective	ADJ
aiua-14101	30	49	cryoprotectant	cryoprotectant	NOUN
aiua-14101	30	50	formulations	formulation	NOUN
aiua-14101	30	51	needed	need	VERB
aiua-14101	30	52	in	in	ADP
aiua-14101	30	53	the	the	DET
aiua-14101	30	54	field	field	NOUN
aiua-14101	30	55	of	of	ADP
aiua-14101	30	56	cryobiology	cryobiology	NOUN
aiua-14101	30	57	.	.	PUNCT
aiua-14101	31	1	materials	material	NOUN
aiua-14101	31	2	and	and	CCONJ
aiua-14101	31	3	methods	method	NOUN
aiua-14101	31	4	chemicals	chemical	NOUN
aiua-14101	31	5	all	all	DET
aiua-14101	31	6	chemicals	chemical	NOUN
aiua-14101	31	7	were	be	AUX
aiua-14101	31	8	purchased	purchase	VERB
aiua-14101	31	9	from	from	ADP
aiua-14101	31	10	sigma	sigma	PROPN
aiua-14101	31	11	chemical	chemical	PROPN
aiua-14101	31	12	company	company	PROPN
aiua-14101	31	13	(	(	PUNCT
aiua-14101	31	14	st	st	PROPN
aiua-14101	31	15	.	.	PROPN
aiua-14101	31	16	louis	louis	PROPN
aiua-14101	31	17	,	,	PUNCT
aiua-14101	31	18	mo	mo	PROPN
aiua-14101	31	19	)	)	PUNCT
aiua-14101	31	20	.	.	PUNCT
aiua-14101	32	1	humon	humon	PROPN
aiua-14101	32	2	tubal	tubal	PROPN
aiua-14101	32	3	fluid	fluid	NOUN
aiua-14101	32	4	(	(	PUNCT
aiua-14101	32	5	htf	htf	PROPN
aiua-14101	32	6	)	)	PUNCT
aiua-14101	32	7	was	be	AUX
aiua-14101	32	8	prepared	prepare	VERB
aiua-14101	32	9	according	accord	VERB
aiua-14101	32	10	to	to	ADP
aiua-14101	32	11	the	the	DET
aiua-14101	32	12	method	method	NOUN
aiua-14101	32	13	of	of	ADP
aiua-14101	32	14	quinn	quinn	PROPN
aiua-14101	32	15	et	et	PROPN
aiua-14101	32	16	al	al	PROPN
aiua-14101	32	17	.	.	PUNCT
aiua-14101	33	1	(	(	PUNCT
aiua-14101	33	2	14	14	NUM
aiua-14101	33	3	)	)	PUNCT
aiua-14101	33	4	.	.	PUNCT
aiua-14101	34	1	cryopreservation	cryopreservation	NOUN
aiua-14101	34	2	agent	agent	NOUN
aiua-14101	34	3	(	(	PUNCT
aiua-14101	34	4	cpa	cpa	PROPN
aiua-14101	34	5	)	)	PUNCT
aiua-14101	34	6	was	be	AUX
aiua-14101	34	7	prepared	prepare	VERB
aiua-14101	34	8	by	by	ADP
aiua-14101	34	9	modifying	modify	VERB
aiua-14101	34	10	the	the	DET
aiua-14101	34	11	method	method	NOUN
aiua-14101	34	12	of	of	ADP
aiua-14101	34	13	nakatsukasa	nakatsukasa	NOUN
aiua-14101	34	14	et	et	PROPN
aiua-14101	34	15	al	al	PROPN
aiua-14101	34	16	.	.	PROPN
aiua-14101	35	1	(	(	PUNCT
aiua-14101	35	2	6	6	NUM
aiua-14101	35	3	)	)	PUNCT
aiua-14101	35	4	.	.	PUNCT
aiua-14101	36	1	23	23	NUM
aiua-14101	36	2	%	%	NOUN
aiua-14101	36	3	fresh	fresh	ADJ
aiua-14101	36	4	chicken	chicken	NOUN
aiua-14101	36	5	egg	egg	NOUN
aiua-14101	36	6	yolk	yolk	NOUN
aiua-14101	36	7	,	,	PUNCT
aiua-14101	36	8	8	8	NUM
aiua-14101	36	9	%	%	NOUN
aiua-14101	36	10	lactose	lactose	NOUN
aiua-14101	36	11	monohydrate	monohydrate	NOUN
aiua-14101	36	12	,	,	PUNCT
aiua-14101	36	13	1	1	NUM
aiua-14101	36	14	mg	mg	NUM
aiua-14101	36	15	/	/	SYM
aiua-14101	36	16	ml	ml	NOUN
aiua-14101	36	17	streptomycin	streptomycin	PROPN
aiua-14101	36	18	sulfate	sulfate	NOUN
aiua-14101	36	19	,	,	PUNCT
aiua-14101	36	20	and	and	CCONJ
aiua-14101	36	21	1000	1000	NUM
aiua-14101	36	22	iu	iu	ADP
aiua-14101	36	23	penicillin	penicillin	PROPN
aiua-14101	36	24	g	g	PROPN
aiua-14101	36	25	potassium	potassium	NOUN
aiua-14101	36	26	were	be	AUX
aiua-14101	36	27	added	add	VERB
aiua-14101	36	28	into	into	ADP
aiua-14101	36	29	23	23	NUM
aiua-14101	36	30	%	%	NOUN
aiua-14101	36	31	fresh	fresh	ADJ
aiua-14101	36	32	chicken	chicken	NOUN
aiua-14101	36	33	egg	egg	NOUN
aiua-14101	36	34	yolk	yolk	NOUN
aiua-14101	36	35	in	in	ADP
aiua-14101	36	36	pure	pure	ADJ
aiua-14101	36	37	distilled	distil	VERB
aiua-14101	36	38	water	water	NOUN
aiua-14101	36	39	and	and	CCONJ
aiua-14101	36	40	mixed	mix	VERB
aiua-14101	36	41	for	for	ADP
aiua-14101	36	42	5	5	NUM
aiua-14101	36	43	min	min	NOUN
aiua-14101	36	44	at	at	ADP
aiua-14101	36	45	+4	+4	PROPN
aiua-14101	36	46	°	°	ADP
aiua-14101	36	47	c	c	NOUN
aiua-14101	36	48	.	.	PUNCT
aiua-14101	37	1	the	the	DET
aiua-14101	37	2	mixture	mixture	NOUN
aiua-14101	37	3	was	be	AUX
aiua-14101	37	4	then	then	ADV
aiua-14101	37	5	centrifuged	centrifuge	VERB
aiua-14101	37	6	at	at	ADP
aiua-14101	37	7	1600	1600	NUM
aiua-14101	37	8	x	x	SYM
aiua-14101	37	9	g	g	NOUN
aiua-14101	37	10	for	for	ADP
aiua-14101	37	11	15	15	NUM
aiua-14101	37	12	min	min	NOUN
aiua-14101	37	13	.	.	PROPN
aiua-14101	38	1	after	after	ADP
aiua-14101	38	2	centrifugation	centrifugation	NOUN
aiua-14101	38	3	,	,	PUNCT
aiua-14101	38	4	the	the	DET
aiua-14101	38	5	supernatant	supernatant	NOUN
aiua-14101	38	6	was	be	AUX
aiua-14101	38	7	collected	collect	VERB
aiua-14101	38	8	,	,	PUNCT
aiua-14101	38	9	and	and	CCONJ
aiua-14101	38	10	0.1	0.1	NUM
aiua-14101	38	11	%	%	NOUN
aiua-14101	38	12	atp	atp	NOUN
aiua-14101	38	13	(	(	PUNCT
aiua-14101	38	14	adenosine	adenosine	NOUN
aiua-14101	38	15	5’-triphosphate	5’-triphosphate	NUM
aiua-14101	38	16	)	)	PUNCT
aiua-14101	38	17	was	be	AUX
aiua-14101	38	18	added	add	VERB
aiua-14101	38	19	.	.	PUNCT
aiua-14101	39	1	the	the	DET
aiua-14101	39	2	resulting	result	VERB
aiua-14101	39	3	diluent	diluent	NOUN
aiua-14101	39	4	was	be	AUX
aiua-14101	39	5	partitioned	partition	VERB
aiua-14101	39	6	,	,	PUNCT
aiua-14101	39	7	and	and	CCONJ
aiua-14101	39	8	25	25	NUM
aiua-14101	39	9	mm	mm	NOUN
aiua-14101	39	10	tryptophan	tryptophan	NOUN
aiua-14101	39	11	(	(	PUNCT
aiua-14101	39	12	sigma	sigma	NOUN
aiua-14101	39	13	-	-	PUNCT
aiua-14101	39	14	aldrich	aldrich	NOUN
aiua-14101	39	15	)	)	PUNCT
aiua-14101	39	16	and	and	CCONJ
aiua-14101	39	17	100	100	NUM
aiua-14101	39	18	mm	mm	NOUN
aiua-14101	39	19	trehalose	trehalose	INTJ
aiua-14101	39	20	(	(	PUNCT
aiua-14101	39	21	sigma	sigma	PROPN
aiua-14101	39	22	-	-	PUNCT
aiua-14101	39	23	aldrich	aldrich	NOUN
aiua-14101	39	24	)	)	PUNCT
aiua-14101	39	25	were	be	AUX
aiua-14101	39	26	added	add	VERB
aiua-14101	39	27	to	to	PART
aiua-14101	39	28	form	form	VERB
aiua-14101	39	29	a	a	DET
aiua-14101	39	30	tryptophan	tryptophan	NOUN
aiua-14101	39	31	(	(	PUNCT
aiua-14101	39	32	tryp	tryp	PROPN
aiua-14101	39	33	)	)	PUNCT
aiua-14101	39	34	study	study	NOUN
aiua-14101	39	35	group	group	NOUN
aiua-14101	39	36	and	and	CCONJ
aiua-14101	39	37	a	a	DET
aiua-14101	39	38	trehalose	trehalose	ADJ
aiua-14101	39	39	(	(	PUNCT
aiua-14101	39	40	treh	treh	NOUN
aiua-14101	39	41	)	)	PUNCT
aiua-14101	39	42	study	study	NOUN
aiua-14101	39	43	group	group	NOUN
aiua-14101	39	44	.	.	PUNCT
aiua-14101	40	1	a	a	DET
aiua-14101	40	2	cpa	cpa	NOUN
aiua-14101	40	3	group	group	NOUN
aiua-14101	40	4	,	,	PUNCT
aiua-14101	40	5	in	in	ADP
aiua-14101	40	6	which	which	PRON
aiua-14101	40	7	no	no	DET
aiua-14101	40	8	addition	addition	NOUN
aiua-14101	40	9	was	be	AUX
aiua-14101	40	10	made	make	VERB
aiua-14101	40	11	,	,	PUNCT
aiua-14101	40	12	was	be	AUX
aiua-14101	40	13	used	use	VERB
aiua-14101	40	14	as	as	ADP
aiua-14101	40	15	the	the	DET
aiua-14101	40	16	control	control	NOUN
aiua-14101	40	17	(	(	PUNCT
aiua-14101	40	18	c	c	NOUN
aiua-14101	40	19	)	)	PUNCT
aiua-14101	40	20	group	group	NOUN
aiua-14101	40	21	.	.	PUNCT
aiua-14101	41	1	lastly	lastly	ADV
aiua-14101	41	2	,	,	PUNCT
aiua-14101	41	3	the	the	DET
aiua-14101	41	4	ph	ph	NOUN
aiua-14101	41	5	of	of	ADP
aiua-14101	41	6	the	the	DET
aiua-14101	41	7	prepared	prepare	VERB
aiua-14101	41	8	study	study	NOUN
aiua-14101	41	9	group	group	NOUN
aiua-14101	41	10	solutions	solution	NOUN
aiua-14101	41	11	was	be	AUX
aiua-14101	41	12	adjusted	adjust	VERB
aiua-14101	41	13	to	to	ADP
aiua-14101	41	14	7.4	7.4	NUM
aiua-14101	41	15	with	with	ADP
aiua-14101	41	16	10	10	NUM
aiua-14101	41	17	%	%	NOUN
aiua-14101	41	18	tris	tris	NOUN
aiua-14101	41	19	aminomethane	aminomethane	PROPN
aiua-14101	41	20	+	+	CCONJ
aiua-14101	41	21	distilled	distil	VERB
aiua-14101	41	22	water	water	NOUN
aiua-14101	41	23	solution	solution	NOUN
aiua-14101	41	24	using	use	VERB
aiua-14101	41	25	a	a	DET
aiua-14101	41	26	ph	ph	ADJ
aiua-14101	41	27	meter	meter	NOUN
aiua-14101	41	28	(	(	PUNCT
aiua-14101	41	29	thermo	thermo	NOUN
aiua-14101	41	30	scientific	scientific	NOUN
aiua-14101	41	31	,	,	PUNCT
aiua-14101	41	32	usa	usa	PROPN
aiua-14101	41	33	)	)	PUNCT
aiua-14101	41	34	.	.	PUNCT
aiua-14101	42	1	animals	animal	NOUN
aiua-14101	42	2	in	in	ADP
aiua-14101	42	3	the	the	DET
aiua-14101	42	4	study	study	NOUN
aiua-14101	42	5	,	,	PUNCT
aiua-14101	42	6	24	24	NUM
aiua-14101	42	7	adult	adult	NOUN
aiua-14101	42	8	,	,	PUNCT
aiua-14101	42	9	male	male	PROPN
aiua-14101	42	10	wistar	wistar	PROPN
aiua-14101	42	11	albino	albino	PROPN
aiua-14101	42	12	rats	rat	NOUN
aiua-14101	42	13	aged	age	VERB
aiua-14101	42	14	1016	1016	NUM
aiua-14101	42	15	weeks	week	NOUN
aiua-14101	42	16	were	be	AUX
aiua-14101	42	17	used	use	VERB
aiua-14101	42	18	.	.	PUNCT
aiua-14101	43	1	rats	rat	NOUN
aiua-14101	43	2	were	be	AUX
aiua-14101	43	3	kept	keep	VERB
aiua-14101	43	4	at	at	ADP
aiua-14101	43	5	room	room	NOUN
aiua-14101	43	6	temperature	temperature	NOUN
aiua-14101	43	7	(	(	PUNCT
aiua-14101	43	8	24	24	NUM
aiua-14101	43	9	±	±	NUM
aiua-14101	43	10	3	3	NUM
aiua-14101	43	11	°	°	NOUN
aiua-14101	43	12	c	c	NOUN
aiua-14101	43	13	)	)	PUNCT
aiua-14101	43	14	and	and	CCONJ
aiua-14101	43	15	a	a	DET
aiua-14101	43	16	relative	relative	ADJ
aiua-14101	43	17	humidity	humidity	NOUN
aiua-14101	43	18	of	of	ADP
aiua-14101	43	19	60	60	NUM
aiua-14101	43	20	%	%	NOUN
aiua-14101	43	21	under	under	ADP
aiua-14101	43	22	12	12	NUM
aiua-14101	43	23	h	h	NOUN
aiua-14101	43	24	light:12	light:12	PRON
aiua-14101	43	25	h	h	NOUN
aiua-14101	43	26	dark	dark	ADJ
aiua-14101	43	27	cycle	cycle	NOUN
aiua-14101	43	28	.	.	PUNCT
aiua-14101	44	1	the	the	DET
aiua-14101	44	2	rats	rat	NOUN
aiua-14101	44	3	were	be	AUX
aiua-14101	44	4	fed	feed	VERB
aiua-14101	44	5	rat	rat	NOUN
aiua-14101	44	6	chow	chow	PROPN
aiua-14101	44	7	(	(	PUNCT
aiua-14101	44	8	pellet	pellet	NOUN
aiua-14101	44	9	feed	feed	NOUN
aiua-14101	44	10	)	)	PUNCT
aiua-14101	44	11	and	and	CCONJ
aiua-14101	44	12	tap	tap	NOUN
aiua-14101	44	13	water	water	NOUN
aiua-14101	44	14	ad	ad	NOUN
aiua-14101	44	15	libitum	libitum	X
aiua-14101	44	16	.	.	PUNCT
aiua-14101	45	1	all	all	DET
aiua-14101	45	2	procedures	procedure	NOUN
aiua-14101	45	3	were	be	AUX
aiua-14101	45	4	carried	carry	VERB
aiua-14101	45	5	out	out	ADP
aiua-14101	45	6	with	with	ADP
aiua-14101	45	7	the	the	DET
aiua-14101	45	8	approval	approval	NOUN
aiua-14101	45	9	(	(	PUNCT
aiua-14101	45	10	ethical	ethical	ADJ
aiua-14101	45	11	approval	approval	NOUN
aiua-14101	45	12	numbered	number	VERB
aiua-14101	45	13	2024/09	2024/09	NUM
aiua-14101	45	14	-	-	SYM
aiua-14101	45	15	01	01	NUM
aiua-14101	45	16	)	)	PUNCT
aiua-14101	45	17	of	of	ADP
aiua-14101	45	18	the	the	DET
aiua-14101	45	19	animal	animal	NOUN
aiua-14101	45	20	experiments	experiment	VERB
aiua-14101	45	21	local	local	ADJ
aiua-14101	45	22	ethics	ethic	NOUN
aiua-14101	45	23	committee	committee	PROPN
aiua-14101	45	24	(	(	PUNCT
aiua-14101	45	25	hadyek	hadyek	NOUN
aiua-14101	45	26	)	)	PUNCT
aiua-14101	45	27	of	of	ADP
aiua-14101	45	28	hatay	hatay	NOUN
aiua-14101	45	29	mustafa	mustafa	PROPN
aiua-14101	45	30	kemal	kemal	PROPN
aiua-14101	45	31	university	university	PROPN
aiua-14101	45	32	.	.	PUNCT
aiua-14101	46	1	collection	collection	NOUN
aiua-14101	46	2	and	and	CCONJ
aiua-14101	46	3	freezing	freezing	NOUN
aiua-14101	46	4	/	/	SYM
aiua-14101	46	5	thawing	thawing	NOUN
aiua-14101	46	6	of	of	ADP
aiua-14101	46	7	spermatozoa	spermatozoa	ADJ
aiua-14101	46	8	rats	rat	NOUN
aiua-14101	46	9	were	be	AUX
aiua-14101	46	10	sacrificed	sacrifice	VERB
aiua-14101	46	11	by	by	ADP
aiua-14101	46	12	cervical	cervical	ADJ
aiua-14101	46	13	dislocation	dislocation	NOUN
aiua-14101	46	14	under	under	ADP
aiua-14101	46	15	anesthesia	anesthesia	PROPN
aiua-14101	46	16	.	.	PUNCT
aiua-14101	47	1	under	under	ADP
aiua-14101	47	2	sterile	sterile	ADJ
aiua-14101	47	3	conditions	condition	NOUN
aiua-14101	47	4	,	,	PUNCT
aiua-14101	47	5	both	both	DET
aiua-14101	47	6	epididymides	epididymis	NOUN
aiua-14101	47	7	were	be	AUX
aiua-14101	47	8	removed	remove	VERB
aiua-14101	47	9	,	,	PUNCT
aiua-14101	47	10	and	and	CCONJ
aiua-14101	47	11	both	both	DET
aiua-14101	47	12	cauda	cauda	NOUN
aiua-14101	47	13	epididymides	epididymis	NOUN
aiua-14101	47	14	were	be	AUX
aiua-14101	47	15	transferred	transfer	VERB
aiua-14101	47	16	into	into	ADP
aiua-14101	47	17	1	1	NUM
aiua-14101	47	18	ml	ml	NOUN
aiua-14101	47	19	cpa	cpa	PROPN
aiua-14101	47	20	placed	place	VERB
aiua-14101	47	21	in	in	ADP
aiua-14101	47	22	35	35	NUM
aiua-14101	47	23	mm	mm	NOUN
aiua-14101	47	24	petri	petri	ADJ
aiua-14101	47	25	dishes	dish	NOUN
aiua-14101	47	26	(	(	PUNCT
aiua-14101	47	27	nunc	nunc	PROPN
aiua-14101	47	28	™	™	PROPN
aiua-14101	47	29	,	,	PUNCT
aiua-14101	47	30	massachusetts	massachusetts	PROPN
aiua-14101	47	31	,	,	PUNCT
aiua-14101	47	32	usa	usa	PROPN
aiua-14101	47	33	)	)	PUNCT
aiua-14101	47	34	at	at	ADP
aiua-14101	47	35	room	room	NOUN
aiua-14101	47	36	temperature	temperature	NOUN
aiua-14101	47	37	.	.	PUNCT
aiua-14101	48	1	rat	rat	NOUN
aiua-14101	48	2	spermatozoa	spermatozoon	NOUN
aiua-14101	48	3	were	be	AUX
aiua-14101	48	4	reconstituted	reconstitute	VERB
aiua-14101	48	5	and	and	CCONJ
aiua-14101	48	6	frozen	frozen	ADJ
aiua-14101	48	7	using	use	VERB
aiua-14101	48	8	a	a	DET
aiua-14101	48	9	modified	modify	VERB
aiua-14101	48	10	version	version	NOUN
aiua-14101	48	11	of	of	ADP
aiua-14101	48	12	the	the	DET
aiua-14101	48	13	protocol	protocol	NOUN
aiua-14101	48	14	described	describe	VERB
aiua-14101	48	15	by	by	ADP
aiua-14101	48	16	nakagata	nakagata	PROPN
aiua-14101	48	17	et	et	PROPN
aiua-14101	48	18	al	al	PROPN
aiua-14101	48	19	.	.	PROPN
aiua-14101	49	1	(	(	PUNCT
aiua-14101	49	2	3	3	NUM
aiua-14101	49	3	)	)	PUNCT
aiua-14101	49	4	.	.	PUNCT
aiua-14101	50	1	the	the	DET
aiua-14101	50	2	cauda	cauda	NOUN
aiua-14101	50	3	epididymides	epididymis	NOUN
aiua-14101	50	4	were	be	AUX
aiua-14101	50	5	immobilized	immobilize	VERB
aiua-14101	50	6	using	use	VERB
aiua-14101	50	7	flat	flat	ADV
aiua-14101	50	8	-	-	PUNCT
aiua-14101	50	9	tipped	tip	VERB
aiua-14101	50	10	forceps	forceps	NOUN
aiua-14101	50	11	under	under	ADP
aiua-14101	50	12	a	a	DET
aiua-14101	50	13	stereo	stereo	ADJ
aiua-14101	50	14	microscope	microscope	NOUN
aiua-14101	50	15	(	(	PUNCT
aiua-14101	50	16	euromex	euromex	NOUN
aiua-14101	50	17	/	/	SYM
aiua-14101	50	18	nexius	nexius	NOUN
aiua-14101	50	19	zoom	zoom	NOUN
aiua-14101	50	20	,	,	PUNCT
aiua-14101	50	21	netherlands	netherlands	PROPN
aiua-14101	50	22	)	)	PUNCT
aiua-14101	50	23	,	,	PUNCT
aiua-14101	50	24	and	and	CCONJ
aiua-14101	50	25	10	10	NUM
aiua-14101	50	26	-	-	SYM
aiua-14101	50	27	12	12	NUM
aiua-14101	50	28	deep	deep	ADJ
aiua-14101	50	29	incisions	incision	NOUN
aiua-14101	50	30	were	be	AUX
aiua-14101	50	31	made	make	VERB
aiua-14101	50	32	in	in	ADP
aiua-14101	50	33	the	the	DET
aiua-14101	50	34	petri	petri	ADJ
aiua-14101	50	35	dish	dish	NOUN
aiua-14101	50	36	using	use	VERB
aiua-14101	50	37	sharp	sharp	ADV
aiua-14101	50	38	-	-	PUNCT
aiua-14101	50	39	tipped	tip	VERB
aiua-14101	50	40	scissors	scissor	NOUN
aiua-14101	50	41	,	,	PUNCT
aiua-14101	50	42	and	and	CCONJ
aiua-14101	50	43	the	the	DET
aiua-14101	50	44	spermatozoa	spermatozoon	NOUN
aiua-14101	50	45	were	be	AUX
aiua-14101	50	46	transferred	transfer	VERB
aiua-14101	50	47	into	into	ADP
aiua-14101	50	48	cpa	cpa	NOUN
aiua-14101	50	49	.	.	PUNCT
aiua-14101	51	1	for	for	ADP
aiua-14101	51	2	the	the	DET
aiua-14101	51	3	release	release	NOUN
aiua-14101	51	4	of	of	ADP
aiua-14101	51	5	spermatozoa	spermatozoon	NOUN
aiua-14101	51	6	from	from	ADP
aiua-14101	51	7	the	the	DET
aiua-14101	51	8	cauda	cauda	NOUN
aiua-14101	51	9	and	and	CCONJ
aiua-14101	51	10	equilibration	equilibration	NOUN
aiua-14101	51	11	,	,	PUNCT
aiua-14101	51	12	the	the	DET
aiua-14101	51	13	petri	petri	ADJ
aiua-14101	51	14	dishes	dish	NOUN
aiua-14101	51	15	were	be	AUX
aiua-14101	51	16	placed	place	VERB
aiua-14101	51	17	on	on	ADP
aiua-14101	51	18	a	a	DET
aiua-14101	51	19	metal	metal	NOUN
aiua-14101	51	20	plate	plate	NOUN
aiua-14101	51	21	on	on	ADP
aiua-14101	51	22	ice	ice	NOUN
aiua-14101	51	23	in	in	ADP
aiua-14101	51	24	a	a	DET
aiua-14101	51	25	styrofoam	styrofoam	NOUN
aiua-14101	51	26	box	box	NOUN
aiua-14101	51	27	and	and	CCONJ
aiua-14101	51	28	incubated	incubate	VERB
aiua-14101	51	29	for	for	ADP
aiua-14101	51	30	10	10	NUM
aiua-14101	51	31	min	min	NOUN
aiua-14101	51	32	.	.	PROPN
aiua-14101	51	33	after	after	ADP
aiua-14101	51	34	incubation	incubation	NOUN
aiua-14101	51	35	,	,	PUNCT
aiua-14101	51	36	0.25	0.25	NUM
aiua-14101	51	37	ml	ml	NOUN
aiua-14101	51	38	straws	straw	NOUN
aiua-14101	51	39	(	(	PUNCT
aiua-14101	51	40	imv	imv	PROPN
aiua-14101	51	41	,	,	PUNCT
aiua-14101	51	42	france	france	PROPN
aiua-14101	51	43	)	)	PUNCT
aiua-14101	51	44	were	be	AUX
aiua-14101	51	45	loaded	load	VERB
aiua-14101	51	46	with	with	ADP
aiua-14101	51	47	30	30	NUM
aiua-14101	51	48	µl	µl	ADP
aiua-14101	51	49	htf	htf	PROPN
aiua-14101	51	50	,	,	PUNCT
aiua-14101	51	51	10	10	NUM
aiua-14101	51	52	mm	mm	NOUN
aiua-14101	51	53	air	air	NOUN
aiua-14101	51	54	,	,	PUNCT
aiua-14101	51	55	150	150	NUM
aiua-14101	51	56	µl	µl	ADP
aiua-14101	51	57	sperm	sperm	NOUN
aiua-14101	51	58	suspension	suspension	NOUN
aiua-14101	51	59	,	,	PUNCT
aiua-14101	51	60	10	10	NUM
aiua-14101	51	61	mm	mm	NUM
aiua-14101	51	62	air	air	NOUN
aiua-14101	51	63	,	,	PUNCT
aiua-14101	51	64	and	and	CCONJ
aiua-14101	51	65	30	30	NUM
aiua-14101	51	66	µl	µl	ADP
aiua-14101	51	67	htf	htf	PROPN
aiua-14101	51	68	,	,	PUNCT
aiua-14101	51	69	respectively	respectively	ADV
aiua-14101	51	70	,	,	PUNCT
aiua-14101	51	71	and	and	CCONJ
aiua-14101	51	72	the	the	DET
aiua-14101	51	73	straws	straw	NOUN
aiua-14101	51	74	were	be	AUX
aiua-14101	51	75	pressed	press	VERB
aiua-14101	51	76	.	.	PUNCT
aiua-14101	52	1	the	the	DET
aiua-14101	52	2	straws	straw	NOUN
aiua-14101	52	3	were	be	AUX
aiua-14101	52	4	placed	place	VERB
aiua-14101	52	5	on	on	ADP
aiua-14101	52	6	a	a	DET
aiua-14101	52	7	metal	metal	NOUN
aiua-14101	52	8	plate	plate	NOUN
aiua-14101	52	9	on	on	ADP
aiua-14101	52	10	ice	ice	NOUN
aiua-14101	52	11	and	and	CCONJ
aiua-14101	52	12	equilibrated	equilibrate	VERB
aiua-14101	52	13	for	for	ADP
aiua-14101	52	14	30	30	NUM
aiua-14101	52	15	min	min	NOUN
aiua-14101	52	16	.	.	PUNCT
aiua-14101	53	1	the	the	DET
aiua-14101	53	2	equipment	equipment	NOUN
aiua-14101	53	3	used	use	VERB
aiua-14101	53	4	during	during	ADP
aiua-14101	53	5	the	the	DET
aiua-14101	53	6	procedure	procedure	NOUN
aiua-14101	53	7	(	(	PUNCT
aiua-14101	53	8	syringe	syringe	NOUN
aiua-14101	53	9	connector	connector	NOUN
aiua-14101	53	10	,	,	PUNCT
aiua-14101	53	11	straws	straw	NOUN
aiua-14101	53	12	,	,	PUNCT
aiua-14101	53	13	and	and	CCONJ
aiua-14101	53	14	htf	htf	PROPN
aiua-14101	53	15	)	)	PUNCT
aiua-14101	53	16	was	be	AUX
aiua-14101	53	17	set	set	VERB
aiua-14101	53	18	at	at	ADP
aiua-14101	53	19	a	a	DET
aiua-14101	53	20	temperature	temperature	NOUN
aiua-14101	53	21	of	of	ADP
aiua-14101	53	22	0	0	NUM
aiua-14101	53	23	°	°	NOUN
aiua-14101	53	24	c	c	NOUN
aiua-14101	53	25	.	.	PUNCT
aiua-14101	54	1	for	for	ADP
aiua-14101	54	2	freezing	freezing	NOUN
aiua-14101	54	3	,	,	PUNCT
aiua-14101	54	4	the	the	DET
aiua-14101	54	5	straws	straw	NOUN
aiua-14101	54	6	were	be	AUX
aiua-14101	54	7	placed	place	VERB
aiua-14101	54	8	in	in	ADP
aiua-14101	54	9	a	a	DET
aiua-14101	54	10	styrofoam	styrofoam	NOUN
aiua-14101	54	11	box	box	NOUN
aiua-14101	54	12	(	(	PUNCT
aiua-14101	54	13	270x220x270	270x220x270	NUM
aiua-14101	54	14	mm	mm	NOUN
aiua-14101	54	15	)	)	PUNCT
aiua-14101	54	16	4	4	NUM
aiua-14101	54	17	cm	cm	NOUN
aiua-14101	54	18	above	above	ADP
aiua-14101	54	19	the	the	DET
aiua-14101	54	20	3.5	3.5	NUM
aiua-14101	54	21	-	-	PUNCT
aiua-14101	54	22	cm	cm	NOUN
aiua-14101	54	23	liquid	liquid	ADJ
aiua-14101	54	24	nitrogen	nitrogen	NOUN
aiua-14101	54	25	level	level	NOUN
aiua-14101	54	26	on	on	ADP
aiua-14101	54	27	a	a	DET
aiua-14101	54	28	straw	straw	NOUN
aiua-14101	54	29	rack	rack	NOUN
aiua-14101	54	30	and	and	CCONJ
aiua-14101	54	31	kept	keep	VERB
aiua-14101	54	32	in	in	ADP
aiua-14101	54	33	liquid	liquid	ADJ
aiua-14101	54	34	nitrogen	nitrogen	NOUN
aiua-14101	54	35	vapor	vapor	NOUN
aiua-14101	54	36	for	for	ADP
aiua-14101	54	37	10	10	NUM
aiua-14101	54	38	min	min	NOUN
aiua-14101	54	39	.	.	PUNCT
aiua-14101	55	1	the	the	DET
aiua-14101	55	2	straws	straw	NOUN
aiua-14101	55	3	were	be	AUX
aiua-14101	55	4	then	then	ADV
aiua-14101	55	5	dipped	dip	VERB
aiua-14101	55	6	in	in	ADP
aiua-14101	55	7	liquid	liquid	ADJ
aiua-14101	55	8	nitrogen	nitrogen	NOUN
aiua-14101	55	9	.	.	PUNCT
aiua-14101	56	1	thawing	thaw	VERB
aiua-14101	56	2	was	be	AUX
aiua-14101	56	3	performed	perform	VERB
aiua-14101	56	4	by	by	ADP
aiua-14101	56	5	dissolving	dissolve	VERB
aiua-14101	56	6	the	the	DET
aiua-14101	56	7	straws	straw	NOUN
aiua-14101	56	8	in	in	ADP
aiua-14101	56	9	a	a	DET
aiua-14101	56	10	37	37	NUM
aiua-14101	56	11	°	°	NOUN
aiua-14101	56	12	c	c	NOUN
aiua-14101	56	13	water	water	NOUN
aiua-14101	56	14	bath	bath	NOUN
aiua-14101	56	15	(	(	PUNCT
aiua-14101	56	16	deconjelator	deconjelator	NOUN
aiua-14101	56	17	,	,	PUNCT
aiua-14101	56	18	cito	cito	PROPN
aiua-14101	56	19	026897	026897	PROPN
aiua-14101	56	20	,	,	PUNCT
aiua-14101	56	21	imv	imv	PROPN
aiua-14101	56	22	,	,	PUNCT
aiua-14101	56	23	france	france	PROPN
aiua-14101	56	24	)	)	PUNCT
aiua-14101	56	25	for	for	ADP
aiua-14101	56	26	30	30	NUM
aiua-14101	56	27	sec	sec	PROPN
aiua-14101	56	28	.	.	PUNCT
aiua-14101	57	1	the	the	DET
aiua-14101	57	2	content	content	NOUN
aiua-14101	57	3	of	of	ADP
aiua-14101	57	4	the	the	DET
aiua-14101	57	5	thawed	thaw	VERB
aiua-14101	57	6	straws	straw	NOUN
aiua-14101	57	7	was	be	AUX
aiua-14101	57	8	transferred	transfer	VERB
aiua-14101	57	9	to	to	ADP
aiua-14101	57	10	1	1	NUM
aiua-14101	57	11	ml	ml	NOUN
aiua-14101	57	12	htf	htf	PROPN
aiua-14101	57	13	in	in	ADP
aiua-14101	57	14	a	a	DET
aiua-14101	57	15	carbon	carbon	NOUN
aiua-14101	57	16	dioxide	dioxide	NOUN
aiua-14101	57	17	incubator	incubator	NOUN
aiua-14101	57	18	(	(	PUNCT
aiua-14101	57	19	37	37	NUM
aiua-14101	57	20	°	°	NOUN
aiua-14101	57	21	c	c	NOUN
aiua-14101	57	22	,	,	PUNCT
aiua-14101	57	23	5	5	NUM
aiua-14101	57	24	%	%	NOUN
aiua-14101	57	25	co2	co2	NOUN
aiua-14101	57	26	)	)	PUNCT
aiua-14101	57	27	.	.	PUNCT
aiua-14101	58	1	assessment	assessment	NOUN
aiua-14101	58	2	of	of	ADP
aiua-14101	58	3	sperm	sperm	NOUN
aiua-14101	58	4	concentration	concentration	NOUN
aiua-14101	58	5	sperm	sperm	NOUN
aiua-14101	58	6	density	density	NOUN
aiua-14101	58	7	was	be	AUX
aiua-14101	58	8	determined	determine	VERB
aiua-14101	58	9	using	use	VERB
aiua-14101	58	10	a	a	DET
aiua-14101	58	11	makler	makler	NOUN
aiua-14101	58	12	counting	counting	NOUN
aiua-14101	58	13	chamber	chamber	PROPN
aiua-14101	58	14	(	(	PUNCT
aiua-14101	58	15	sefi	sefi	NOUN
aiua-14101	58	16	-	-	PUNCT
aiua-14101	58	17	medical	medical	ADJ
aiua-14101	58	18	instruments	instrument	NOUN
aiua-14101	58	19	,	,	PUNCT
aiua-14101	58	20	haifa	haifa	PROPN
aiua-14101	58	21	,	,	PUNCT
aiua-14101	58	22	israel	israel	PROPN
aiua-14101	58	23	)	)	PUNCT
aiua-14101	58	24	following	follow	VERB
aiua-14101	58	25	the	the	DET
aiua-14101	58	26	guidelines	guideline	NOUN
aiua-14101	58	27	of	of	ADP
aiua-14101	58	28	the	the	DET
aiua-14101	58	29	world	world	NOUN
aiua-14101	58	30	health	health	NOUN
aiua-14101	58	31	organization	organization	NOUN
aiua-14101	58	32	(	(	PUNCT
aiua-14101	58	33	15	15	NUM
aiua-14101	58	34	)	)	PUNCT
aiua-14101	58	35	.	.	PUNCT
aiua-14101	59	1	sperm	sperm	NOUN
aiua-14101	59	2	samples	sample	NOUN
aiua-14101	59	3	were	be	AUX
aiua-14101	59	4	diluted	dilute	VERB
aiua-14101	59	5	1:10	1:10	NUM
aiua-14101	59	6	with	with	ADP
aiua-14101	59	7	phosphate	phosphate	ADJ
aiua-14101	59	8	buffered	buffer	VERB
aiua-14101	59	9	saline	saline	NOUN
aiua-14101	59	10	(	(	PUNCT
aiua-14101	59	11	pbs	pbs	PROPN
aiua-14101	59	12	)	)	PUNCT
aiua-14101	59	13	fixation	fixation	NOUN
aiua-14101	59	14	medium	medium	NOUN
aiua-14101	59	15	containing	contain	VERB
aiua-14101	59	16	10	10	NUM
aiua-14101	59	17	mm	mm	NOUN
aiua-14101	59	18	formaldehyde	formaldehyde	NOUN
aiua-14101	59	19	and	and	CCONJ
aiua-14101	59	20	immobilized	immobilize	VERB
aiua-14101	59	21	.	.	PUNCT
aiua-14101	60	1	from	from	ADP
aiua-14101	60	2	the	the	DET
aiua-14101	60	3	fixed	fix	VERB
aiua-14101	60	4	mixture	mixture	NOUN
aiua-14101	60	5	,	,	PUNCT
aiua-14101	60	6	10	10	NUM
aiua-14101	60	7	µl	µl	NOUN
aiua-14101	60	8	was	be	AUX
aiua-14101	60	9	taken	take	VERB
aiua-14101	60	10	and	and	CCONJ
aiua-14101	60	11	the	the	DET
aiua-14101	60	12	cells	cell	NOUN
aiua-14101	60	13	in	in	ADP
aiua-14101	60	14	10	10	NUM
aiua-14101	60	15	frames	frame	NOUN
aiua-14101	60	16	were	be	AUX
aiua-14101	60	17	counted	count	VERB
aiua-14101	60	18	under	under	ADP
aiua-14101	60	19	a	a	DET
aiua-14101	60	20	20x	20x	NOUN
aiua-14101	60	21	objective	objective	NOUN
aiua-14101	60	22	in	in	ADP
aiua-14101	60	23	olympus	olympus	PROPN
aiua-14101	60	24	cx31	cx31	PROPN
aiua-14101	60	25	(	(	PUNCT
aiua-14101	60	26	japan	japan	PROPN
aiua-14101	60	27	)	)	PUNCT
aiua-14101	60	28	microscope	microscope	NOUN
aiua-14101	60	29	and	and	CCONJ
aiua-14101	60	30	the	the	DET
aiua-14101	60	31	density	density	NOUN
aiua-14101	60	32	was	be	AUX
aiua-14101	60	33	calculated	calculate	VERB
aiua-14101	60	34	in	in	ADP
aiua-14101	60	35	millions	million	NOUN
aiua-14101	60	36	/	/	SYM
aiua-14101	60	37	ml	ml	NOUN
aiua-14101	60	38	.	.	PUNCT
aiua-14101	61	1	each	each	DET
aiua-14101	61	2	sample	sample	NOUN
aiua-14101	61	3	was	be	AUX
aiua-14101	61	4	measured	measure	VERB
aiua-14101	61	5	three	three	NUM
aiua-14101	61	6	times	time	NOUN
aiua-14101	61	7	,	,	PUNCT
aiua-14101	61	8	and	and	CCONJ
aiua-14101	61	9	the	the	DET
aiua-14101	61	10	results	result	NOUN
aiua-14101	61	11	were	be	AUX
aiua-14101	61	12	averaged	average	VERB
aiua-14101	61	13	.	.	PUNCT
aiua-14101	62	1	assessment	assessment	NOUN
aiua-14101	62	2	of	of	ADP
aiua-14101	62	3	sperm	sperm	NOUN
aiua-14101	62	4	motility	motility	NOUN
aiua-14101	62	5	sperm	sperm	NOUN
aiua-14101	62	6	motility	motility	NOUN
aiua-14101	62	7	was	be	AUX
aiua-14101	62	8	analyzed	analyze	VERB
aiua-14101	62	9	using	use	VERB
aiua-14101	62	10	a	a	DET
aiua-14101	62	11	makler	makler	NOUN
aiua-14101	62	12	counting	counting	NOUN
aiua-14101	62	13	chamber	chamber	PROPN
aiua-14101	62	14	(	(	PUNCT
aiua-14101	62	15	sefi	sefi	NOUN
aiua-14101	62	16	-	-	PUNCT
aiua-14101	62	17	medical	medical	ADJ
aiua-14101	62	18	instruments	instrument	NOUN
aiua-14101	62	19	,	,	PUNCT
aiua-14101	62	20	haifa	haifa	PROPN
aiua-14101	62	21	,	,	PUNCT
aiua-14101	62	22	israel	israel	PROPN
aiua-14101	62	23	)	)	PUNCT
aiua-14101	62	24	according	accord	VERB
aiua-14101	62	25	to	to	ADP
aiua-14101	62	26	the	the	DET
aiua-14101	62	27	guidelines	guideline	NOUN
aiua-14101	62	28	of	of	ADP
aiua-14101	62	29	who	who	PRON
aiua-14101	62	30	(	(	PUNCT
aiua-14101	62	31	15	15	NUM
aiua-14101	62	32	)	)	PUNCT
aiua-14101	62	33	.	.	PUNCT
aiua-14101	63	1	briefly	briefly	NOUN
aiua-14101	63	2	,	,	PUNCT
aiua-14101	63	3	20	20	NUM
aiua-14101	63	4	µl	µl	NOUN
aiua-14101	63	5	of	of	ADP
aiua-14101	63	6	htf	htf	NOUN
aiua-14101	63	7	and	and	CCONJ
aiua-14101	63	8	sperm	sperm	NOUN
aiua-14101	63	9	mixture	mixture	NOUN
aiua-14101	63	10	was	be	AUX
aiua-14101	63	11	taken	take	VERB
aiua-14101	63	12	and	and	CCONJ
aiua-14101	63	13	placed	place	VERB
aiua-14101	63	14	on	on	ADP
aiua-14101	63	15	a	a	DET
aiua-14101	63	16	makler	makler	NOUN
aiua-14101	63	17	slide	slide	NOUN
aiua-14101	63	18	preheated	preheat	VERB
aiua-14101	63	19	to	to	ADP
aiua-14101	63	20	37	37	NUM
aiua-14101	63	21	°	°	NOUN
aiua-14101	63	22	c	c	NOUN
aiua-14101	63	23	.	.	PUNCT
aiua-14101	64	1	samples	sample	NOUN
aiua-14101	64	2	were	be	AUX
aiua-14101	64	3	examined	examine	VERB
aiua-14101	64	4	under	under	ADP
aiua-14101	64	5	a	a	DET
aiua-14101	64	6	20x	20x	NOUN
aiua-14101	64	7	objective	objective	NOUN
aiua-14101	64	8	in	in	ADP
aiua-14101	64	9	a	a	DET
aiua-14101	64	10	phase	phase	NOUN
aiua-14101	64	11	contrast	contrast	NOUN
aiua-14101	64	12	microscope	microscope	NOUN
aiua-14101	64	13	with	with	ADP
aiua-14101	64	14	a	a	DET
aiua-14101	64	15	heating	heating	NOUN
aiua-14101	64	16	stage	stage	NOUN
aiua-14101	64	17	(	(	PUNCT
aiua-14101	64	18	olympus	olympus	PROPN
aiua-14101	64	19	cx31	cx31	PROPN
aiua-14101	64	20	,	,	PUNCT
aiua-14101	64	21	japan	japan	PROPN
aiua-14101	64	22	)	)	PUNCT
aiua-14101	64	23	.	.	PUNCT
aiua-14101	65	1	within	within	ADP
aiua-14101	65	2	100	100	NUM
aiua-14101	65	3	small	small	ADJ
aiua-14101	65	4	squares	square	NOUN
aiua-14101	65	5	on	on	ADP
aiua-14101	65	6	the	the	DET
aiua-14101	65	7	makler	makler	NOUN
aiua-14101	65	8	slide	slide	NOUN
aiua-14101	65	9	,	,	PUNCT
aiua-14101	65	10	the	the	DET
aiua-14101	65	11	ratio	ratio	NOUN
aiua-14101	65	12	of	of	ADP
aiua-14101	65	13	spermatozoa	spermatozoon	NOUN
aiua-14101	65	14	showing	show	VERB
aiua-14101	65	15	linear	linear	PROPN
aiua-14101	65	16	forward	forward	ADJ
aiua-14101	65	17	movement	movement	NOUN
aiua-14101	65	18	to	to	ADP
aiua-14101	65	19	spermatozoa	spermatozoa	VERB
aiua-14101	65	20	with	with	ADP
aiua-14101	65	21	other	other	ADJ
aiua-14101	65	22	types	type	NOUN
aiua-14101	65	23	of	of	ADP
aiua-14101	65	24	movement	movement	NOUN
aiua-14101	65	25	(	(	PUNCT
aiua-14101	65	26	tremor	tremor	NOUN
aiua-14101	65	27	,	,	PUNCT
aiua-14101	65	28	rotation	rotation	NOUN
aiua-14101	65	29	,	,	PUNCT
aiua-14101	65	30	or	or	CCONJ
aiua-14101	65	31	immobility	immobility	NOUN
aiua-14101	65	32	)	)	PUNCT
aiua-14101	65	33	was	be	AUX
aiua-14101	65	34	calculated	calculate	VERB
aiua-14101	65	35	.	.	PUNCT
aiua-14101	66	1	measurement	measurement	NOUN
aiua-14101	66	2	was	be	AUX
aiua-14101	66	3	repeated	repeat	VERB
aiua-14101	66	4	three	three	NUM
aiua-14101	66	5	times	time	NOUN
aiua-14101	66	6	for	for	ADP
aiua-14101	66	7	each	each	DET
aiua-14101	66	8	sample	sample	NOUN
aiua-14101	66	9	,	,	PUNCT
aiua-14101	66	10	and	and	CCONJ
aiua-14101	66	11	the	the	DET
aiua-14101	66	12	results	result	NOUN
aiua-14101	66	13	were	be	AUX
aiua-14101	66	14	averaged	average	VERB
aiua-14101	66	15	.	.	PUNCT
aiua-14101	67	1	sperm	sperm	NOUN
aiua-14101	67	2	motility	motility	NOUN
aiua-14101	67	3	was	be	AUX
aiua-14101	67	4	expressed	express	VERB
aiua-14101	67	5	as	as	ADP
aiua-14101	67	6	a	a	DET
aiua-14101	67	7	percentage	percentage	NOUN
aiua-14101	67	8	(	(	PUNCT
aiua-14101	67	9	%	%	NOUN
aiua-14101	67	10	)	)	PUNCT
aiua-14101	67	11	.	.	PUNCT
aiua-14101	68	1	assessment	assessment	NOUN
aiua-14101	68	2	of	of	ADP
aiua-14101	68	3	sperm	sperm	NOUN
aiua-14101	68	4	viability	viability	NOUN
aiua-14101	68	5	sperm	sperm	NOUN
aiua-14101	68	6	viability	viability	NOUN
aiua-14101	68	7	was	be	AUX
aiua-14101	68	8	analyzed	analyze	VERB
aiua-14101	68	9	using	use	VERB
aiua-14101	68	10	the	the	DET
aiua-14101	68	11	eosin	eosin	NOUN
aiua-14101	68	12	-	-	PUNCT
aiua-14101	68	13	nigrosin	nigrosin	NOUN
aiua-14101	68	14	staining	stain	VERB
aiua-14101	68	15	method	method	NOUN
aiua-14101	68	16	described	describe	VERB
aiua-14101	68	17	by	by	ADP
aiua-14101	68	18	agarwal	agarwal	PROPN
aiua-14101	68	19	et	et	PROPN
aiua-14101	68	20	al	al	PROPN
aiua-14101	68	21	.	.	PUNCT
aiua-14101	69	1	(	(	PUNCT
aiua-14101	69	2	16	16	NUM
aiua-14101	69	3	)	)	PUNCT
aiua-14101	69	4	.	.	PUNCT
aiua-14101	70	1	10	10	NUM
aiua-14101	70	2	µl	µl	NOUN
aiua-14101	70	3	of	of	ADP
aiua-14101	70	4	sperm	sperm	NOUN
aiua-14101	70	5	samples	sample	NOUN
aiua-14101	70	6	were	be	AUX
aiua-14101	70	7	taken	take	VERB
aiua-14101	70	8	and	and	CCONJ
aiua-14101	70	9	mixed	mix	VERB
aiua-14101	70	10	with	with	ADP
aiua-14101	70	11	20	20	NUM
aiua-14101	70	12	µl	µl	NOUN
aiua-14101	70	13	of	of	ADP
aiua-14101	70	14	1	1	NUM
aiua-14101	70	15	%	%	NOUN
aiua-14101	70	16	eosin	eosin	NOUN
aiua-14101	70	17	and	and	CCONJ
aiua-14101	70	18	10	10	NUM
aiua-14101	70	19	%	%	NOUN
aiua-14101	70	20	nigrosin	nigrosin	NOUN
aiua-14101	70	21	solutions	solution	NOUN
aiua-14101	70	22	.	.	PUNCT
aiua-14101	71	1	the	the	DET
aiua-14101	71	2	mixture	mixture	NOUN
aiua-14101	71	3	was	be	AUX
aiua-14101	71	4	swabbed	swab	VERB
aiua-14101	71	5	onto	onto	ADP
aiua-14101	71	6	slides	slide	NOUN
aiua-14101	71	7	and	and	CCONJ
aiua-14101	71	8	dried	dry	VERB
aiua-14101	71	9	on	on	ADP
aiua-14101	71	10	a	a	DET
aiua-14101	71	11	surface	surface	NOUN
aiua-14101	71	12	at	at	ADP
aiua-14101	71	13	60	60	NUM
aiua-14101	71	14	°	°	ADP
aiua-14101	71	15	c	c	NOUN
aiua-14101	71	16	for	for	ADP
aiua-14101	71	17	23	23	NUM
aiua-14101	71	18	sec	sec	PROPN
aiua-14101	71	19	.	.	PUNCT
aiua-14101	72	1	analyses	analysis	NOUN
aiua-14101	72	2	were	be	AUX
aiua-14101	72	3	performed	perform	VERB
aiua-14101	72	4	under	under	ADP
aiua-14101	72	5	a	a	DET
aiua-14101	72	6	phase	phase	NOUN
aiua-14101	72	7	contrast	contrast	NOUN
aiua-14101	72	8	microscope	microscope	NOUN
aiua-14101	72	9	(	(	PUNCT
aiua-14101	72	10	olympus	olympus	PROPN
aiua-14101	72	11	cx31	cx31	PROPN
aiua-14101	72	12	,	,	PUNCT
aiua-14101	72	13	japan	japan	PROPN
aiua-14101	72	14	)	)	PUNCT
aiua-14101	72	15	and	and	CCONJ
aiua-14101	72	16	a	a	DET
aiua-14101	72	17	40x	40x	NOUN
aiua-14101	72	18	objective	objective	NOUN
aiua-14101	72	19	by	by	ADP
aiua-14101	72	20	counting	count	VERB
aiua-14101	72	21	at	at	ADV
aiua-14101	72	22	least	least	ADV
aiua-14101	72	23	300	300	NUM
aiua-14101	72	24	spermatozoa	spermatozoon	NOUN
aiua-14101	72	25	.	.	PUNCT
aiua-14101	73	1	spermatozoa	spermatozoon	NOUN
aiua-14101	73	2	with	with	ADP
aiua-14101	73	3	red	red	ADJ
aiua-14101	73	4	or	or	CCONJ
aiua-14101	73	5	dark	dark	ADJ
aiua-14101	73	6	pink	pink	ADJ
aiua-14101	73	7	heads	head	NOUN
aiua-14101	73	8	were	be	AUX
aiua-14101	73	9	classified	classify	VERB
aiua-14101	73	10	as	as	ADP
aiua-14101	73	11	non	non	ADJ
aiua-14101	73	12	-	-	ADJ
aiua-14101	73	13	viable	viable	ADJ
aiua-14101	73	14	,	,	PUNCT
aiua-14101	73	15	while	while	SCONJ
aiua-14101	73	16	those	those	PRON
aiua-14101	73	17	with	with	ADP
aiua-14101	73	18	unstained	unstained	ADJ
aiua-14101	73	19	heads	head	NOUN
aiua-14101	73	20	were	be	AUX
aiua-14101	73	21	considered	consider	VERB
aiua-14101	73	22	viable	viable	ADJ
aiua-14101	73	23	.	.	PUNCT
aiua-14101	74	1	viability	viability	NOUN
aiua-14101	74	2	ratio	ratio	NOUN
aiua-14101	74	3	was	be	AUX
aiua-14101	74	4	calculated	calculate	VERB
aiua-14101	74	5	as	as	ADP
aiua-14101	74	6	percentage	percentage	NOUN
aiua-14101	74	7	(	(	PUNCT
aiua-14101	74	8	%	%	NOUN
aiua-14101	74	9	)	)	PUNCT
aiua-14101	74	10	.	.	PUNCT
aiua-14101	75	1	assessment	assessment	NOUN
aiua-14101	75	2	of	of	ADP
aiua-14101	75	3	sperm	sperm	NOUN
aiua-14101	75	4	plasma	plasma	NOUN
aiua-14101	75	5	membrane	membrane	NOUN
aiua-14101	75	6	integrity	integrity	NOUN
aiua-14101	75	7	hypo	hypo	ADJ
aiua-14101	75	8	-	-	ADJ
aiua-14101	75	9	osmotic	osmotic	ADJ
aiua-14101	75	10	swelling	swell	VERB
aiua-14101	75	11	test	test	NOUN
aiua-14101	75	12	(	(	PUNCT
aiua-14101	75	13	host	host	NOUN
aiua-14101	75	14	)	)	PUNCT
aiua-14101	75	15	described	describe	VERB
aiua-14101	75	16	by	by	ADP
aiua-14101	75	17	jeyendran	jeyendran	NOUN
aiua-14101	75	18	archivio	archivio	PROPN
aiua-14101	75	19	italiano	italiano	X
aiua-14101	75	20	di	di	X
aiua-14101	75	21	urologia	urologia	PROPN
aiua-14101	75	22	e	e	PROPN
aiua-14101	75	23	andrologia	andrologia	NOUN
aiua-14101	75	24	2025	2025	NUM
aiua-14101	75	25	;	;	PUNCT
aiua-14101	75	26	97(3):14101	97(3):14101	NUM
aiua-14101	75	27	3	3	NUM
aiua-14101	75	28	tryptophan	tryptophan	NOUN
aiua-14101	75	29	and	and	CCONJ
aiua-14101	75	30	trehalose	trehalose	NOUN
aiua-14101	75	31	on	on	ADP
aiua-14101	75	32	post	post	ADJ
aiua-14101	75	33	-	-	ADJ
aiua-14101	75	34	thaw	thaw	ADJ
aiua-14101	75	35	sperm	sperm	NOUN
aiua-14101	75	36	et	et	PROPN
aiua-14101	75	37	al	al	PROPN
aiua-14101	75	38	.	.	PUNCT
aiua-14101	76	1	(	(	PUNCT
aiua-14101	76	2	17	17	NUM
aiua-14101	76	3	)	)	PUNCT
aiua-14101	76	4	was	be	AUX
aiua-14101	76	5	performed	perform	VERB
aiua-14101	76	6	to	to	PART
aiua-14101	76	7	evaluate	evaluate	VERB
aiua-14101	76	8	the	the	DET
aiua-14101	76	9	functional	functional	ADJ
aiua-14101	76	10	stability	stability	NOUN
aiua-14101	76	11	of	of	ADP
aiua-14101	76	12	the	the	DET
aiua-14101	76	13	plasma	plasma	NOUN
aiua-14101	76	14	membrane	membrane	NOUN
aiua-14101	76	15	integrity	integrity	NOUN
aiua-14101	76	16	of	of	ADP
aiua-14101	76	17	spermatozoa	spermatozoon	NOUN
aiua-14101	76	18	.	.	PUNCT
aiua-14101	77	1	100	100	NUM
aiua-14101	77	2	µl	µl	NOUN
aiua-14101	77	3	of	of	ADP
aiua-14101	77	4	host	host	NOUN
aiua-14101	77	5	solution	solution	NOUN
aiua-14101	77	6	(	(	PUNCT
aiua-14101	77	7	1.1	1.1	NUM
aiua-14101	77	8	g	g	NOUN
aiua-14101	77	9	fructose	fructose	NOUN
aiua-14101	77	10	+	+	CCONJ
aiua-14101	77	11	0.55	0.55	NUM
aiua-14101	77	12	g	g	PROPN
aiua-14101	77	13	sodium	sodium	NOUN
aiua-14101	77	14	citrate	citrate	NOUN
aiua-14101	77	15	+	+	CCONJ
aiua-14101	77	16	100	100	NUM
aiua-14101	77	17	ml	ml	ADP
aiua-14101	77	18	distilled	distil	VERB
aiua-14101	77	19	water	water	NOUN
aiua-14101	77	20	)	)	PUNCT
aiua-14101	77	21	,	,	PUNCT
aiua-14101	77	22	of	of	ADP
aiua-14101	77	23	which	which	PRON
aiua-14101	77	24	osmotic	osmotic	ADJ
aiua-14101	77	25	pressure	pressure	NOUN
aiua-14101	77	26	was	be	AUX
aiua-14101	77	27	measured	measure	VERB
aiua-14101	77	28	with	with	ADP
aiua-14101	77	29	an	an	DET
aiua-14101	77	30	osmometer	osmometer	NOUN
aiua-14101	77	31	(	(	PUNCT
aiua-14101	77	32	osmomat	osmomat	ADJ
aiua-14101	77	33	3000	3000	NUM
aiua-14101	77	34	,	,	PUNCT
aiua-14101	77	35	gonotec	gonotec	NOUN
aiua-14101	77	36	,	,	PUNCT
aiua-14101	77	37	usa	usa	PROPN
aiua-14101	77	38	)	)	PUNCT
aiua-14101	77	39	,	,	PUNCT
aiua-14101	77	40	and	and	CCONJ
aiua-14101	77	41	10	10	NUM
aiua-14101	77	42	µl	µl	NOUN
aiua-14101	77	43	of	of	ADP
aiua-14101	77	44	the	the	DET
aiua-14101	77	45	sperm	sperm	NOUN
aiua-14101	77	46	sample	sample	NOUN
aiua-14101	77	47	were	be	AUX
aiua-14101	77	48	transferred	transfer	VERB
aiua-14101	77	49	into	into	ADP
aiua-14101	77	50	a	a	DET
aiua-14101	77	51	1.5	1.5	NUM
aiua-14101	77	52	ml	ml	NOUN
aiua-14101	77	53	eppendorf	eppendorf	PROPN
aiua-14101	77	54	tube	tube	PROPN
aiua-14101	77	55	.	.	PUNCT
aiua-14101	78	1	the	the	DET
aiua-14101	78	2	mixture	mixture	NOUN
aiua-14101	78	3	was	be	AUX
aiua-14101	78	4	kept	keep	VERB
aiua-14101	78	5	in	in	ADP
aiua-14101	78	6	an	an	DET
aiua-14101	78	7	incubator	incubator	NOUN
aiua-14101	78	8	at	at	ADP
aiua-14101	78	9	37	37	NUM
aiua-14101	78	10	°	°	ADP
aiua-14101	78	11	c	c	NOUN
aiua-14101	78	12	for	for	ADP
aiua-14101	78	13	45	45	NUM
aiua-14101	78	14	min	min	NOUN
aiua-14101	78	15	.	.	PROPN
aiua-14101	79	1	after	after	ADP
aiua-14101	79	2	incubation	incubation	NOUN
aiua-14101	79	3	,	,	PUNCT
aiua-14101	79	4	the	the	DET
aiua-14101	79	5	samples	sample	NOUN
aiua-14101	79	6	were	be	AUX
aiua-14101	79	7	examined	examine	VERB
aiua-14101	79	8	on	on	ADP
aiua-14101	79	9	a	a	DET
aiua-14101	79	10	slide	slide	NOUN
aiua-14101	79	11	preheated	preheat	VERB
aiua-14101	79	12	to	to	ADP
aiua-14101	79	13	37	37	NUM
aiua-14101	79	14	°	°	ADP
aiua-14101	79	15	c	c	NOUN
aiua-14101	79	16	under	under	ADP
aiua-14101	79	17	a	a	DET
aiua-14101	79	18	phase	phase	NOUN
aiua-14101	79	19	contrast	contrast	NOUN
aiua-14101	79	20	microscope	microscope	NOUN
aiua-14101	79	21	(	(	PUNCT
aiua-14101	79	22	olympus	olympus	PROPN
aiua-14101	79	23	cx31	cx31	PROPN
aiua-14101	79	24	,	,	PUNCT
aiua-14101	79	25	japan	japan	PROPN
aiua-14101	79	26	)	)	PUNCT
aiua-14101	79	27	and	and	CCONJ
aiua-14101	79	28	a	a	DET
aiua-14101	79	29	40x	40x	NOUN
aiua-14101	79	30	objective	objective	NOUN
aiua-14101	79	31	.	.	PUNCT
aiua-14101	80	1	spermatozoa	spermatozoon	NOUN
aiua-14101	80	2	with	with	ADP
aiua-14101	80	3	swelling	swell	VERB
aiua-14101	80	4	or	or	CCONJ
aiua-14101	80	5	curling	curl	VERB
aiua-14101	80	6	in	in	ADP
aiua-14101	80	7	the	the	DET
aiua-14101	80	8	tail	tail	NOUN
aiua-14101	80	9	were	be	AUX
aiua-14101	80	10	categorized	categorize	VERB
aiua-14101	80	11	as	as	ADP
aiua-14101	80	12	host	host	NOUN
aiua-14101	80	13	positive	positive	ADJ
aiua-14101	80	14	(	(	PUNCT
aiua-14101	80	15	+	+	NOUN
aiua-14101	80	16	)	)	PUNCT
aiua-14101	80	17	and	and	CCONJ
aiua-14101	80	18	those	those	PRON
aiua-14101	80	19	without	without	ADP
aiua-14101	80	20	any	any	DET
aiua-14101	80	21	morphological	morphological	ADJ
aiua-14101	80	22	change	change	NOUN
aiua-14101	80	23	were	be	AUX
aiua-14101	80	24	categorized	categorize	VERB
aiua-14101	80	25	as	as	ADP
aiua-14101	80	26	host	host	NOUN
aiua-14101	80	27	negative	negative	ADJ
aiua-14101	80	28	(	(	PUNCT
aiua-14101	80	29	-	-	PUNCT
aiua-14101	80	30	)	)	PUNCT
aiua-14101	80	31	.	.	PUNCT
aiua-14101	81	1	from	from	ADP
aiua-14101	81	2	each	each	DET
aiua-14101	81	3	sample	sample	NOUN
aiua-14101	81	4	,	,	PUNCT
aiua-14101	81	5	a	a	DET
aiua-14101	81	6	total	total	NOUN
aiua-14101	81	7	of	of	ADP
aiua-14101	81	8	200	200	NUM
aiua-14101	81	9	spermatozoa	spermatozoon	NOUN
aiua-14101	81	10	were	be	AUX
aiua-14101	81	11	counted	count	VERB
aiua-14101	81	12	,	,	PUNCT
aiua-14101	81	13	and	and	CCONJ
aiua-14101	81	14	the	the	DET
aiua-14101	81	15	ratio	ratio	NOUN
aiua-14101	81	16	of	of	ADP
aiua-14101	81	17	host	host	NOUN
aiua-14101	81	18	positive	positive	ADJ
aiua-14101	81	19	spermatozoa	spermatozoon	NOUN
aiua-14101	81	20	was	be	AUX
aiua-14101	81	21	recorded	record	VERB
aiua-14101	81	22	as	as	ADP
aiua-14101	81	23	percentage	percentage	NOUN
aiua-14101	81	24	(	(	PUNCT
aiua-14101	81	25	%	%	NOUN
aiua-14101	81	26	)	)	PUNCT
aiua-14101	81	27	.	.	PUNCT
aiua-14101	82	1	acrosome	acrosome	ADJ
aiua-14101	82	2	assessment	assessment	NOUN
aiua-14101	82	3	abnormal	abnormal	ADJ
aiua-14101	82	4	acrosome	acrosome	NOUN
aiua-14101	82	5	examination	examination	NOUN
aiua-14101	82	6	was	be	AUX
aiua-14101	82	7	performed	perform	VERB
aiua-14101	82	8	based	base	VERB
aiua-14101	82	9	on	on	ADP
aiua-14101	82	10	the	the	DET
aiua-14101	82	11	method	method	NOUN
aiua-14101	82	12	described	describe	VERB
aiua-14101	82	13	by	by	ADP
aiua-14101	82	14	somfai	somfai	NOUN
aiua-14101	82	15	et	et	PROPN
aiua-14101	82	16	al	al	PROPN
aiua-14101	82	17	.	.	PROPN
aiua-14101	83	1	(	(	PUNCT
aiua-14101	83	2	18	18	NUM
aiua-14101	83	3	)	)	PUNCT
aiua-14101	83	4	.	.	PUNCT
aiua-14101	84	1	hancock	hancock	PROPN
aiua-14101	84	2	’s	’s	PART
aiua-14101	84	3	solution	solution	NOUN
aiua-14101	84	4	(	(	PUNCT
aiua-14101	84	5	19	19	NUM
aiua-14101	84	6	)	)	PUNCT
aiua-14101	84	7	was	be	AUX
aiua-14101	84	8	used	use	VERB
aiua-14101	84	9	in	in	ADP
aiua-14101	84	10	the	the	DET
aiua-14101	84	11	examination	examination	NOUN
aiua-14101	84	12	.	.	PUNCT
aiua-14101	85	1	500	500	NUM
aiua-14101	85	2	µl	µl	NOUN
aiua-14101	85	3	of	of	ADP
aiua-14101	85	4	hancock	hancock	PROPN
aiua-14101	85	5	’s	’s	PART
aiua-14101	85	6	solution	solution	NOUN
aiua-14101	85	7	was	be	AUX
aiua-14101	85	8	transferred	transfer	VERB
aiua-14101	85	9	into	into	ADP
aiua-14101	85	10	an	an	DET
aiua-14101	85	11	eppendorf	eppendorf	ADJ
aiua-14101	85	12	tube	tube	NOUN
aiua-14101	85	13	,	,	PUNCT
aiua-14101	85	14	and	and	CCONJ
aiua-14101	85	15	50	50	NUM
aiua-14101	85	16	µl	µl	NOUN
aiua-14101	85	17	of	of	ADP
aiua-14101	85	18	sperm	sperm	NOUN
aiua-14101	85	19	sample	sample	NOUN
aiua-14101	85	20	was	be	AUX
aiua-14101	85	21	added	add	VERB
aiua-14101	85	22	.	.	PUNCT
aiua-14101	86	1	50	50	NUM
aiua-14101	86	2	µl	µl	NOUN
aiua-14101	86	3	of	of	ADP
aiua-14101	86	4	the	the	DET
aiua-14101	86	5	mixture	mixture	NOUN
aiua-14101	86	6	was	be	AUX
aiua-14101	86	7	placed	place	VERB
aiua-14101	86	8	on	on	ADP
aiua-14101	86	9	a	a	DET
aiua-14101	86	10	slide	slide	NOUN
aiua-14101	86	11	and	and	CCONJ
aiua-14101	86	12	covered	cover	VERB
aiua-14101	86	13	with	with	ADP
aiua-14101	86	14	a	a	DET
aiua-14101	86	15	coverslip	coverslip	NOUN
aiua-14101	86	16	.	.	PUNCT
aiua-14101	87	1	morphological	morphological	ADJ
aiua-14101	87	2	evaluation	evaluation	NOUN
aiua-14101	87	3	of	of	ADP
aiua-14101	87	4	the	the	DET
aiua-14101	87	5	slides	slide	NOUN
aiua-14101	87	6	was	be	AUX
aiua-14101	87	7	performed	perform	VERB
aiua-14101	87	8	under	under	ADP
aiua-14101	87	9	a	a	DET
aiua-14101	87	10	microscope	microscope	NOUN
aiua-14101	87	11	and	and	CCONJ
aiua-14101	87	12	a	a	DET
aiua-14101	87	13	100x	100x	NUM
aiua-14101	87	14	objective	objective	NOUN
aiua-14101	87	15	using	use	VERB
aiua-14101	87	16	immersion	immersion	NOUN
aiua-14101	87	17	oil	oil	NOUN
aiua-14101	87	18	(	(	PUNCT
aiua-14101	87	19	20	20	NUM
aiua-14101	87	20	)	)	PUNCT
aiua-14101	87	21	.	.	PUNCT
aiua-14101	88	1	a	a	DET
aiua-14101	88	2	total	total	NOUN
aiua-14101	88	3	of	of	ADP
aiua-14101	88	4	300	300	NUM
aiua-14101	88	5	spermatozoa	spermatozoon	NOUN
aiua-14101	88	6	were	be	AUX
aiua-14101	88	7	examined	examine	VERB
aiua-14101	88	8	,	,	PUNCT
aiua-14101	88	9	and	and	CCONJ
aiua-14101	88	10	the	the	DET
aiua-14101	88	11	ratio	ratio	NOUN
aiua-14101	88	12	of	of	ADP
aiua-14101	88	13	spermatozoa	spermatozoon	NOUN
aiua-14101	88	14	with	with	ADP
aiua-14101	88	15	acrosome	acrosome	ADJ
aiua-14101	88	16	damage	damage	NOUN
aiua-14101	88	17	was	be	AUX
aiua-14101	88	18	noted	note	VERB
aiua-14101	88	19	as	as	ADP
aiua-14101	88	20	percentage	percentage	NOUN
aiua-14101	88	21	(	(	PUNCT
aiua-14101	88	22	%	%	NOUN
aiua-14101	88	23	)	)	PUNCT
aiua-14101	88	24	.	.	PUNCT
aiua-14101	89	1	assessment	assessment	NOUN
aiua-14101	89	2	of	of	ADP
aiua-14101	89	3	apoptosis	apoptosis	NOUN
aiua-14101	89	4	annexin	annexin	NOUN
aiua-14101	89	5	-	-	PUNCT
aiua-14101	89	6	v	v	NOUN
aiua-14101	89	7	fitc	fitc	NOUN
aiua-14101	89	8	/	/	SYM
aiua-14101	89	9	pi	pi	NOUN
aiua-14101	89	10	apoptosis	apoptosis	NOUN
aiua-14101	89	11	kit	kit	NOUN
aiua-14101	89	12	(	(	PUNCT
aiua-14101	89	13	elabscience	elabscience	NOUN
aiua-14101	89	14	,	,	PUNCT
aiua-14101	89	15	e	e	NOUN
aiua-14101	89	16	-	-	PUNCT
aiua-14101	89	17	cka211	cka211	PROPN
aiua-14101	89	18	)	)	PUNCT
aiua-14101	89	19	was	be	AUX
aiua-14101	89	20	used	use	VERB
aiua-14101	89	21	for	for	ADP
aiua-14101	89	22	apoptosis	apoptosis	NOUN
aiua-14101	89	23	examination	examination	NOUN
aiua-14101	89	24	.	.	PUNCT
aiua-14101	90	1	kit	kit	NOUN
aiua-14101	90	2	and	and	CCONJ
aiua-14101	90	3	staining	stain	VERB
aiua-14101	90	4	procedures	procedure	NOUN
aiua-14101	90	5	were	be	AUX
aiua-14101	90	6	performed	perform	VERB
aiua-14101	90	7	according	accord	VERB
aiua-14101	90	8	to	to	ADP
aiua-14101	90	9	the	the	DET
aiua-14101	90	10	instructions	instruction	NOUN
aiua-14101	90	11	of	of	ADP
aiua-14101	90	12	the	the	DET
aiua-14101	90	13	manufacturer	manufacturer	NOUN
aiua-14101	90	14	.	.	PUNCT
aiua-14101	91	1	apoptotic	apoptotic	ADJ
aiua-14101	91	2	cells	cell	NOUN
aiua-14101	91	3	were	be	AUX
aiua-14101	91	4	detected	detect	VERB
aiua-14101	91	5	with	with	ADP
aiua-14101	91	6	annexin	annexin	NOUN
aiua-14101	91	7	v	v	NOUN
aiua-14101	91	8	-	-	PUNCT
aiua-14101	91	9	fitc	fitc	NOUN
aiua-14101	91	10	stain	stain	NOUN
aiua-14101	91	11	,	,	PUNCT
aiua-14101	91	12	while	while	SCONJ
aiua-14101	91	13	dna	dna	PROPN
aiua-14101	91	14	of	of	ADP
aiua-14101	91	15	necrotic	necrotic	ADJ
aiua-14101	91	16	cells	cell	NOUN
aiua-14101	91	17	with	with	ADP
aiua-14101	91	18	impaired	impaired	ADJ
aiua-14101	91	19	membrane	membrane	NOUN
aiua-14101	91	20	integrity	integrity	NOUN
aiua-14101	91	21	was	be	AUX
aiua-14101	91	22	stained	stain	VERB
aiua-14101	91	23	with	with	ADP
aiua-14101	91	24	propidium	propidium	NOUN
aiua-14101	91	25	iodide	iodide	NOUN
aiua-14101	91	26	(	(	PUNCT
aiua-14101	91	27	pi	pi	NOUN
aiua-14101	91	28	)	)	PUNCT
aiua-14101	91	29	.	.	PUNCT
aiua-14101	92	1	the	the	DET
aiua-14101	92	2	slides	slide	NOUN
aiua-14101	92	3	prepared	prepare	VERB
aiua-14101	92	4	for	for	ADP
aiua-14101	92	5	analysis	analysis	NOUN
aiua-14101	92	6	were	be	AUX
aiua-14101	92	7	examined	examine	VERB
aiua-14101	92	8	under	under	ADP
aiua-14101	92	9	a	a	DET
aiua-14101	92	10	fluorescence	fluorescence	NOUN
aiua-14101	92	11	microscope	microscope	NOUN
aiua-14101	92	12	(	(	PUNCT
aiua-14101	92	13	eclipse	eclipse	PROPN
aiua-14101	92	14	ni	ni	PROPN
aiua-14101	92	15	,	,	PUNCT
aiua-14101	92	16	nikon	nikon	ADJ
aiua-14101	92	17	)	)	PUNCT
aiua-14101	92	18	and	and	CCONJ
aiua-14101	92	19	20x	20x	NOUN
aiua-14101	92	20	and	and	CCONJ
aiua-14101	92	21	40x	40x	NOUN
aiua-14101	92	22	objectives	objective	NOUN
aiua-14101	92	23	using	use	VERB
aiua-14101	92	24	fitc	fitc	NOUN
aiua-14101	92	25	/	/	SYM
aiua-14101	92	26	pi	pi	NOUN
aiua-14101	92	27	filters	filter	NOUN
aiua-14101	92	28	(	(	PUNCT
aiua-14101	92	29	excitation	excitation	NOUN
aiua-14101	92	30	wavelength	wavelength	NOUN
aiua-14101	92	31	of	of	ADP
aiua-14101	92	32	490	490	NUM
aiua-14101	92	33	nm	nm	NOUN
aiua-14101	92	34	,	,	PUNCT
aiua-14101	92	35	emission	emission	NOUN
aiua-14101	92	36	wavelength	wavelength	NOUN
aiua-14101	92	37	of	of	ADP
aiua-14101	92	38	520	520	NUM
aiua-14101	92	39	nm	nm	NOUN
aiua-14101	92	40	)	)	PUNCT
aiua-14101	92	41	.	.	PUNCT
aiua-14101	93	1	cells	cell	NOUN
aiua-14101	93	2	were	be	AUX
aiua-14101	93	3	classified	classify	VERB
aiua-14101	93	4	as	as	SCONJ
aiua-14101	93	5	follows	follow	VERB
aiua-14101	93	6	:	:	PUNCT
aiua-14101	93	7	annexin	annexin	NOUN
aiua-14101	93	8	v	v	NOUN
aiua-14101	93	9	-	-	PUNCT
aiua-14101	93	10	fitc	fitc	NOUN
aiua-14101	93	11	positive	positive	ADJ
aiua-14101	93	12	(	(	PUNCT
aiua-14101	93	13	av+	av+	NOUN
aiua-14101	93	14	)	)	PUNCT
aiua-14101	93	15	and	and	CCONJ
aiua-14101	93	16	propidium	propidium	NOUN
aiua-14101	93	17	iodide	iodide	NOUN
aiua-14101	93	18	negative	negative	ADJ
aiua-14101	93	19	(	(	PUNCT
aiua-14101	93	20	pi-	pi-	NOUN
aiua-14101	93	21	)	)	PUNCT
aiua-14101	93	22	cells	cell	NOUN
aiua-14101	93	23	(	(	PUNCT
aiua-14101	93	24	green	green	ADJ
aiua-14101	93	25	)	)	PUNCT
aiua-14101	93	26	were	be	AUX
aiua-14101	93	27	defined	define	VERB
aiua-14101	93	28	as	as	ADP
aiua-14101	93	29	apoptotic	apoptotic	ADJ
aiua-14101	93	30	cells	cell	NOUN
aiua-14101	93	31	.	.	PUNCT
aiua-14101	94	1	a	a	DET
aiua-14101	94	2	total	total	NOUN
aiua-14101	94	3	of	of	ADP
aiua-14101	94	4	200	200	NUM
aiua-14101	94	5	cells	cell	NOUN
aiua-14101	94	6	were	be	AUX
aiua-14101	94	7	counted	count	VERB
aiua-14101	94	8	,	,	PUNCT
aiua-14101	94	9	and	and	CCONJ
aiua-14101	94	10	apoptosis	apoptosis	NOUN
aiua-14101	94	11	ratios	ratio	NOUN
aiua-14101	94	12	were	be	AUX
aiua-14101	94	13	recorded	record	VERB
aiua-14101	94	14	as	as	ADP
aiua-14101	94	15	percentage	percentage	NOUN
aiua-14101	94	16	(	(	PUNCT
aiua-14101	94	17	%	%	NOUN
aiua-14101	94	18	)	)	PUNCT
aiua-14101	94	19	.	.	PUNCT
aiua-14101	95	1	total	total	ADJ
aiua-14101	95	2	rna	rna	NOUN
aiua-14101	95	3	isolation	isolation	NOUN
aiua-14101	95	4	sperm	sperm	NOUN
aiua-14101	95	5	suspension	suspension	NOUN
aiua-14101	95	6	were	be	AUX
aiua-14101	95	7	washed	wash	VERB
aiua-14101	95	8	with	with	ADP
aiua-14101	95	9	pbs	pbs	PROPN
aiua-14101	95	10	and	and	CCONJ
aiua-14101	95	11	centrifuged	centrifuge	VERB
aiua-14101	95	12	at	at	ADP
aiua-14101	95	13	150	150	NUM
aiua-14101	95	14	×	×	NOUN
aiua-14101	95	15	g	g	NOUN
aiua-14101	95	16	for	for	ADP
aiua-14101	95	17	10	10	NUM
aiua-14101	95	18	minutes	minute	NOUN
aiua-14101	95	19	at	at	ADP
aiua-14101	95	20	4	4	NUM
aiua-14101	95	21	°	°	NOUN
aiua-14101	95	22	c	c	NOUN
aiua-14101	95	23	.	.	PUNCT
aiua-14101	96	1	to	to	PART
aiua-14101	96	2	eliminate	eliminate	VERB
aiua-14101	96	3	somatic	somatic	ADJ
aiua-14101	96	4	cell	cell	NOUN
aiua-14101	96	5	contamination	contamination	NOUN
aiua-14101	96	6	,	,	PUNCT
aiua-14101	96	7	the	the	DET
aiua-14101	96	8	pellets	pellet	NOUN
aiua-14101	96	9	were	be	AUX
aiua-14101	96	10	treated	treat	VERB
aiua-14101	96	11	with	with	ADP
aiua-14101	96	12	a	a	DET
aiua-14101	96	13	somatic	somatic	ADJ
aiua-14101	96	14	cell	cell	NOUN
aiua-14101	96	15	lysis	lysis	NOUN
aiua-14101	96	16	buffer	buffer	NOUN
aiua-14101	96	17	(	(	PUNCT
aiua-14101	96	18	0.05	0.05	NUM
aiua-14101	96	19	%	%	NOUN
aiua-14101	96	20	sodium	sodium	NOUN
aiua-14101	96	21	dodecyl	dodecyl	NOUN
aiua-14101	96	22	sulfate	sulfate	VERB
aiua-14101	96	23	and	and	CCONJ
aiua-14101	96	24	0.25	0.25	NUM
aiua-14101	96	25	%	%	NOUN
aiua-14101	96	26	triton	triton	PROPN
aiua-14101	96	27	x-100	x-100	PROPN
aiua-14101	96	28	in	in	ADP
aiua-14101	96	29	distilled	distil	VERB
aiua-14101	96	30	water	water	NOUN
aiua-14101	96	31	)	)	PUNCT
aiua-14101	96	32	and	and	CCONJ
aiua-14101	96	33	incubated	incubate	VERB
aiua-14101	96	34	on	on	ADP
aiua-14101	96	35	ice	ice	NOUN
aiua-14101	96	36	for	for	ADP
aiua-14101	96	37	60	60	NUM
aiua-14101	96	38	minutes	minute	NOUN
aiua-14101	96	39	.	.	PUNCT
aiua-14101	97	1	total	total	ADJ
aiua-14101	97	2	rna	rna	PROPN
aiua-14101	97	3	was	be	AUX
aiua-14101	97	4	extracted	extract	VERB
aiua-14101	97	5	using	use	VERB
aiua-14101	97	6	trizol	trizol	ADJ
aiua-14101	97	7	reagent	reagent	NOUN
aiua-14101	97	8	(	(	PUNCT
aiua-14101	97	9	trizol	trizol	VERB
aiua-14101	97	10	®	®	NOUN
aiua-14101	97	11	reagent	reagent	NOUN
aiua-14101	97	12	,	,	PUNCT
aiua-14101	97	13	ambion	ambion	NOUN
aiua-14101	97	14	)	)	PUNCT
aiua-14101	97	15	following	follow	VERB
aiua-14101	97	16	the	the	DET
aiua-14101	97	17	manufacturer	manufacturer	NOUN
aiua-14101	97	18	’s	’s	PART
aiua-14101	97	19	protocol	protocol	NOUN
aiua-14101	97	20	.	.	PUNCT
aiua-14101	98	1	carefully	carefully	ADV
aiua-14101	98	2	dissected	dissect	VERB
aiua-14101	98	3	semen	semen	NOUN
aiua-14101	98	4	sample	sample	NOUN
aiua-14101	98	5	was	be	AUX
aiua-14101	98	6	submerged	submerge	VERB
aiua-14101	98	7	in	in	ADP
aiua-14101	98	8	1	1	NUM
aiua-14101	98	9	ml	ml	NOUN
aiua-14101	98	10	of	of	ADP
aiua-14101	98	11	trizol	trizol	ADJ
aiua-14101	98	12	reagent	reagent	ADJ
aiua-14101	98	13	and	and	CCONJ
aiua-14101	98	14	homogenized	homogenize	VERB
aiua-14101	98	15	for	for	ADP
aiua-14101	98	16	45	45	NUM
aiua-14101	98	17	seconds	second	NOUN
aiua-14101	98	18	with	with	ADP
aiua-14101	98	19	a	a	DET
aiua-14101	98	20	homogenizer	homogenizer	NOUN
aiua-14101	98	21	.	.	PUNCT
aiua-14101	99	1	the	the	DET
aiua-14101	99	2	homogenate	homogenate	NOUN
aiua-14101	99	3	was	be	AUX
aiua-14101	99	4	incubated	incubate	VERB
aiua-14101	99	5	for	for	ADP
aiua-14101	99	6	5	5	NUM
aiua-14101	99	7	minutes	minute	NOUN
aiua-14101	99	8	and	and	CCONJ
aiua-14101	99	9	added	add	VERB
aiua-14101	99	10	200	200	NUM
aiua-14101	99	11	μl	μl	ADP
aiua-14101	99	12	chloroform	chloroform	NOUN
aiua-14101	99	13	;	;	PUNCT
aiua-14101	99	14	shaken	shake	VERB
aiua-14101	99	15	vigorously	vigorously	ADV
aiua-14101	99	16	.	.	PUNCT
aiua-14101	100	1	the	the	DET
aiua-14101	100	2	mixture	mixture	NOUN
aiua-14101	100	3	was	be	AUX
aiua-14101	100	4	centrifuged	centrifuge	VERB
aiua-14101	100	5	at	at	ADP
aiua-14101	100	6	12000	12000	NUM
aiua-14101	100	7	×	×	NOUN
aiua-14101	100	8	g	g	NOUN
aiua-14101	100	9	at	at	ADP
aiua-14101	100	10	4	4	NUM
aiua-14101	100	11	°	°	ADP
aiua-14101	100	12	c	c	NOUN
aiua-14101	100	13	for	for	ADP
aiua-14101	100	14	15	15	NUM
aiua-14101	100	15	minutes	minute	NOUN
aiua-14101	100	16	.	.	PUNCT
aiua-14101	101	1	following	follow	VERB
aiua-14101	101	2	this	this	DET
aiua-14101	101	3	centrifugation	centrifugation	NOUN
aiua-14101	101	4	step	step	NOUN
aiua-14101	101	5	,	,	PUNCT
aiua-14101	101	6	the	the	DET
aiua-14101	101	7	upper	upper	ADJ
aiua-14101	101	8	aqueous	aqueous	ADJ
aiua-14101	101	9	layer	layer	NOUN
aiua-14101	101	10	was	be	AUX
aiua-14101	101	11	transferred	transfer	VERB
aiua-14101	101	12	to	to	ADP
aiua-14101	101	13	another	another	DET
aiua-14101	101	14	nuclease	nuclease	NOUN
aiua-14101	101	15	-	-	PUNCT
aiua-14101	101	16	free	free	ADJ
aiua-14101	101	17	1.5	1.5	NUM
aiua-14101	101	18	ml	ml	NOUN
aiua-14101	101	19	tube	tube	NOUN
aiua-14101	101	20	and	and	CCONJ
aiua-14101	101	21	added	add	VERB
aiua-14101	101	22	500	500	NUM
aiua-14101	101	23	μl	μl	ADP
aiua-14101	101	24	of	of	ADP
aiua-14101	101	25	isopropanol	isopropanol	NOUN
aiua-14101	101	26	;	;	PUNCT
aiua-14101	101	27	shaken	shake	VERB
aiua-14101	101	28	vigorously	vigorously	ADV
aiua-14101	101	29	.	.	PUNCT
aiua-14101	102	1	the	the	DET
aiua-14101	102	2	sample	sample	NOUN
aiua-14101	102	3	was	be	AUX
aiua-14101	102	4	centrifuged	centrifuge	VERB
aiua-14101	102	5	at	at	ADP
aiua-14101	102	6	12000	12000	NUM
aiua-14101	102	7	×	×	NOUN
aiua-14101	102	8	g	g	NOUN
aiua-14101	102	9	at	at	ADP
aiua-14101	102	10	4	4	NUM
aiua-14101	102	11	°	°	ADP
aiua-14101	102	12	c	c	NOUN
aiua-14101	102	13	for	for	ADP
aiua-14101	102	14	10	10	NUM
aiua-14101	102	15	minutes	minute	NOUN
aiua-14101	102	16	to	to	PART
aiua-14101	102	17	pellet	pellet	VERB
aiua-14101	102	18	the	the	DET
aiua-14101	102	19	precipitated	precipitate	VERB
aiua-14101	102	20	rna	rna	NOUN
aiua-14101	102	21	.	.	PUNCT
aiua-14101	103	1	the	the	DET
aiua-14101	103	2	rna	rna	NOUN
aiua-14101	103	3	-	-	PUNCT
aiua-14101	103	4	containing	contain	VERB
aiua-14101	103	5	pellet	pellet	NOUN
aiua-14101	103	6	was	be	AUX
aiua-14101	103	7	washed	wash	VERB
aiua-14101	103	8	twice	twice	ADV
aiua-14101	103	9	with	with	ADP
aiua-14101	103	10	500	500	NUM
aiua-14101	103	11	μl	μl	ADP
aiua-14101	103	12	of	of	ADP
aiua-14101	103	13	70	70	NUM
aiua-14101	103	14	%	%	NOUN
aiua-14101	103	15	ethanol	ethanol	NOUN
aiua-14101	103	16	and	and	CCONJ
aiua-14101	103	17	centrifuged	centrifuge	VERB
aiua-14101	103	18	at	at	ADP
aiua-14101	103	19	7500	7500	NUM
aiua-14101	103	20	×	×	NOUN
aiua-14101	103	21	g	g	NOUN
aiua-14101	103	22	at	at	ADP
aiua-14101	103	23	4	4	NUM
aiua-14101	103	24	°	°	ADP
aiua-14101	103	25	c	c	NOUN
aiua-14101	103	26	for	for	ADP
aiua-14101	103	27	5	5	NUM
aiua-14101	103	28	minutes	minute	NOUN
aiua-14101	103	29	.	.	PUNCT
aiua-14101	104	1	final	final	ADJ
aiua-14101	104	2	rna	rna	NOUN
aiua-14101	104	3	pellet	pellet	NOUN
aiua-14101	104	4	was	be	AUX
aiua-14101	104	5	washed	wash	VERB
aiua-14101	104	6	with	with	ADP
aiua-14101	104	7	500	500	NUM
aiua-14101	104	8	μl	μl	ADP
aiua-14101	104	9	of	of	ADP
aiua-14101	104	10	99	99	NUM
aiua-14101	104	11	%	%	NOUN
aiua-14101	104	12	ethanol	ethanol	NOUN
aiua-14101	104	13	and	and	CCONJ
aiua-14101	104	14	centrifuged	centrifuge	VERB
aiua-14101	104	15	at	at	ADP
aiua-14101	104	16	7500	7500	NUM
aiua-14101	104	17	×	×	NOUN
aiua-14101	104	18	g	g	NOUN
aiua-14101	104	19	at	at	ADP
aiua-14101	104	20	4	4	NUM
aiua-14101	104	21	°	°	ADP
aiua-14101	104	22	c	c	NOUN
aiua-14101	104	23	for	for	ADP
aiua-14101	104	24	5	5	NUM
aiua-14101	104	25	minutes	minute	NOUN
aiua-14101	104	26	.	.	PUNCT
aiua-14101	105	1	the	the	DET
aiua-14101	105	2	rna	rna	NOUN
aiua-14101	105	3	-	-	PUNCT
aiua-14101	105	4	containing	contain	VERB
aiua-14101	105	5	pellet	pellet	NOUN
aiua-14101	105	6	was	be	AUX
aiua-14101	105	7	air	air	NOUN
aiua-14101	105	8	-	-	PUNCT
aiua-14101	105	9	dried	dry	VERB
aiua-14101	105	10	and	and	CCONJ
aiua-14101	105	11	dissolved	dissolve	VERB
aiua-14101	105	12	in	in	ADP
aiua-14101	105	13	30	30	NUM
aiua-14101	105	14	ml	ml	NOUN
aiua-14101	105	15	of	of	ADP
aiua-14101	105	16	nuclease	nuclease	NOUN
aiua-14101	105	17	-	-	PUNCT
aiua-14101	105	18	free	free	ADJ
aiua-14101	105	19	water	water	NOUN
aiua-14101	105	20	.	.	PUNCT
aiua-14101	106	1	the	the	DET
aiua-14101	106	2	rna	rna	NOUN
aiua-14101	106	3	samples	sample	NOUN
aiua-14101	106	4	were	be	AUX
aiua-14101	106	5	stored	store	VERB
aiua-14101	106	6	at	at	ADP
aiua-14101	106	7	-80ºc	-80ºc	PROPN
aiua-14101	106	8	for	for	ADP
aiua-14101	106	9	downstream	downstream	ADJ
aiua-14101	106	10	application	application	NOUN
aiua-14101	106	11	later	later	ADV
aiua-14101	106	12	.	.	PUNCT
aiua-14101	107	1	the	the	DET
aiua-14101	107	2	concentration	concentration	NOUN
aiua-14101	107	3	and	and	CCONJ
aiua-14101	107	4	purity	purity	NOUN
aiua-14101	107	5	of	of	ADP
aiua-14101	107	6	rna	rna	NOUN
aiua-14101	107	7	were	be	AUX
aiua-14101	107	8	assessed	assess	VERB
aiua-14101	107	9	by	by	ADP
aiua-14101	107	10	measuring	measure	VERB
aiua-14101	107	11	absorbance	absorbance	NOUN
aiua-14101	107	12	at	at	ADP
aiua-14101	107	13	260/280	260/280	PROPN
aiua-14101	107	14	nm	nm	NOUN
aiua-14101	107	15	using	use	VERB
aiua-14101	107	16	a	a	DET
aiua-14101	107	17	nanodrop	nanodrop	NOUN
aiua-14101	107	18	™	™	ADJ
aiua-14101	107	19	1000	1000	NUM
aiua-14101	107	20	spectrophotometer	spectrophotometer	NOUN
aiua-14101	107	21	(	(	PUNCT
aiua-14101	107	22	thermo	thermo	NOUN
aiua-14101	107	23	fisher	fisher	PROPN
aiua-14101	107	24	scientific	scientific	PROPN
aiua-14101	107	25	,	,	PUNCT
aiua-14101	107	26	usa	usa	PROPN
aiua-14101	107	27	)	)	PUNCT
aiua-14101	107	28	.	.	PUNCT
aiua-14101	108	1	cdna	cdna	PROPN
aiua-14101	108	2	synthesis	synthesis	NOUN
aiua-14101	108	3	complementary	complementary	ADJ
aiua-14101	108	4	dna	dna	NOUN
aiua-14101	108	5	(	(	PUNCT
aiua-14101	108	6	cdna	cdna	PROPN
aiua-14101	108	7	)	)	PUNCT
aiua-14101	108	8	was	be	AUX
aiua-14101	108	9	synthesized	synthesize	VERB
aiua-14101	108	10	from	from	ADP
aiua-14101	108	11	1	1	NUM
aiua-14101	108	12	μg	μg	NOUN
aiua-14101	108	13	of	of	ADP
aiua-14101	108	14	total	total	ADJ
aiua-14101	108	15	rna	rna	NOUN
aiua-14101	108	16	using	use	VERB
aiua-14101	108	17	the	the	DET
aiua-14101	108	18	revertaid	revertaid	ADJ
aiua-14101	108	19	first	first	ADJ
aiua-14101	108	20	strand	strand	PROPN
aiua-14101	108	21	cdna	cdna	PROPN
aiua-14101	108	22	synthesis	synthesis	NOUN
aiua-14101	108	23	kit	kit	NOUN
aiua-14101	108	24	(	(	PUNCT
aiua-14101	108	25	thermo	thermo	NOUN
aiua-14101	108	26	fisher	fisher	PROPN
aiua-14101	108	27	scientific	scientific	PROPN
aiua-14101	108	28	,	,	PUNCT
aiua-14101	108	29	usa	usa	PROPN
aiua-14101	108	30	.	.	PUNCT
aiua-14101	109	1	the	the	DET
aiua-14101	109	2	reaction	reaction	NOUN
aiua-14101	109	3	included	include	VERB
aiua-14101	109	4	1	1	NUM
aiua-14101	109	5	μl	μl	ADP
aiua-14101	109	6	of	of	ADP
aiua-14101	109	7	oligo(dt	oligo(dt	PROPN
aiua-14101	109	8	)	)	PUNCT
aiua-14101	109	9	(	(	PUNCT
aiua-14101	109	10	18	18	NUM
aiua-14101	109	11	)	)	PUNCT
aiua-14101	109	12	primer	primer	NOUN
aiua-14101	109	13	and	and	CCONJ
aiua-14101	109	14	1	1	NUM
aiua-14101	109	15	μl	μl	ADP
aiua-14101	109	16	of	of	ADP
aiua-14101	109	17	random	random	ADJ
aiua-14101	109	18	hexamer	hexamer	NOUN
aiua-14101	109	19	primer	primer	NOUN
aiua-14101	109	20	,	,	PUNCT
aiua-14101	109	21	which	which	PRON
aiua-14101	109	22	were	be	AUX
aiua-14101	109	23	mixed	mix	VERB
aiua-14101	109	24	with	with	ADP
aiua-14101	109	25	the	the	DET
aiua-14101	109	26	rna	rna	NOUN
aiua-14101	109	27	and	and	CCONJ
aiua-14101	109	28	incubated	incubate	VERB
aiua-14101	109	29	at	at	ADP
aiua-14101	109	30	65	65	NUM
aiua-14101	109	31	°	°	ADP
aiua-14101	109	32	c	c	NOUN
aiua-14101	109	33	for	for	ADP
aiua-14101	109	34	5	5	NUM
aiua-14101	109	35	minutes	minute	NOUN
aiua-14101	109	36	,	,	PUNCT
aiua-14101	109	37	followed	follow	VERB
aiua-14101	109	38	by	by	ADP
aiua-14101	109	39	immediate	immediate	ADJ
aiua-14101	109	40	cooling	cooling	NOUN
aiua-14101	109	41	on	on	ADP
aiua-14101	109	42	ice	ice	NOUN
aiua-14101	109	43	.	.	PUNCT
aiua-14101	110	1	subsequently	subsequently	ADV
aiua-14101	110	2	,	,	PUNCT
aiua-14101	110	3	8	8	NUM
aiua-14101	110	4	μl	μl	NOUN
aiua-14101	110	5	of	of	ADP
aiua-14101	110	6	cdna	cdna	PROPN
aiua-14101	110	7	synthesis	synthesis	NOUN
aiua-14101	110	8	mix	mix	NOUN
aiua-14101	110	9	(	(	PUNCT
aiua-14101	110	10	comprising	comprise	VERB
aiua-14101	110	11	5x	5x	NUM
aiua-14101	110	12	reaction	reaction	NOUN
aiua-14101	110	13	buffer	buffer	NOUN
aiua-14101	110	14	,	,	PUNCT
aiua-14101	110	15	ribolock	ribolock	NOUN
aiua-14101	110	16	rnase	rnase	NOUN
aiua-14101	110	17	inhibitor	inhibitor	NOUN
aiua-14101	110	18	,	,	PUNCT
aiua-14101	110	19	dntp	dntp	NOUN
aiua-14101	110	20	mix	mix	NOUN
aiua-14101	110	21	,	,	PUNCT
aiua-14101	110	22	and	and	CCONJ
aiua-14101	110	23	revertaid	revertaid	PROPN
aiua-14101	110	24	m	m	PROPN
aiua-14101	110	25	-	-	PUNCT
aiua-14101	110	26	mulv	mulv	ADJ
aiua-14101	110	27	reverse	reverse	NOUN
aiua-14101	110	28	transcriptase	transcriptase	NOUN
aiua-14101	110	29	)	)	PUNCT
aiua-14101	110	30	was	be	AUX
aiua-14101	110	31	added	add	VERB
aiua-14101	110	32	.	.	PUNCT
aiua-14101	111	1	the	the	DET
aiua-14101	111	2	reaction	reaction	NOUN
aiua-14101	111	3	was	be	AUX
aiua-14101	111	4	incubated	incubate	VERB
aiua-14101	111	5	at	at	ADP
aiua-14101	111	6	42	42	NUM
aiua-14101	111	7	°	°	ADP
aiua-14101	111	8	c	c	NOUN
aiua-14101	111	9	for	for	ADP
aiua-14101	111	10	60	60	NUM
aiua-14101	111	11	minutes	minute	NOUN
aiua-14101	111	12	,	,	PUNCT
aiua-14101	111	13	followed	follow	VERB
aiua-14101	111	14	by	by	ADP
aiua-14101	111	15	25	25	NUM
aiua-14101	111	16	°	°	NOUN
aiua-14101	111	17	c	c	NOUN
aiua-14101	111	18	for	for	ADP
aiua-14101	111	19	5	5	NUM
aiua-14101	111	20	minutes	minute	NOUN
aiua-14101	111	21	,	,	PUNCT
aiua-14101	111	22	and	and	CCONJ
aiua-14101	111	23	terminated	terminate	VERB
aiua-14101	111	24	by	by	ADP
aiua-14101	111	25	heating	heat	VERB
aiua-14101	111	26	at	at	ADP
aiua-14101	111	27	70	70	NUM
aiua-14101	111	28	°	°	ADP
aiua-14101	111	29	c	c	NOUN
aiua-14101	111	30	for	for	ADP
aiua-14101	111	31	5	5	NUM
aiua-14101	111	32	minutes	minute	NOUN
aiua-14101	111	33	.	.	PUNCT
aiua-14101	112	1	quantitation	quantitation	NOUN
aiua-14101	112	2	of	of	ADP
aiua-14101	112	3	gene	gene	NOUN
aiua-14101	112	4	expression	expression	NOUN
aiua-14101	112	5	by	by	ADP
aiua-14101	112	6	rt	rt	NOUN
aiua-14101	112	7	-	-	PUNCT
aiua-14101	112	8	qpcr	qpcr	ADV
aiua-14101	112	9	reverse	reverse	VERB
aiua-14101	112	10	transcription	transcription	NOUN
aiua-14101	112	11	-	-	PUNCT
aiua-14101	112	12	quantitative	quantitative	ADJ
aiua-14101	112	13	polymerase	polymerase	NOUN
aiua-14101	112	14	chain	chain	NOUN
aiua-14101	112	15	reaction	reaction	NOUN
aiua-14101	112	16	(	(	PUNCT
aiua-14101	112	17	rt	rt	NOUN
aiua-14101	112	18	-	-	PUNCT
aiua-14101	112	19	qpcr	qpcr	ADV
aiua-14101	112	20	)	)	PUNCT
aiua-14101	112	21	was	be	AUX
aiua-14101	112	22	performed	perform	VERB
aiua-14101	112	23	using	use	VERB
aiua-14101	112	24	realq	realq	PROPN
aiua-14101	112	25	plus	plus	CCONJ
aiua-14101	112	26	2x	2x	NUM
aiua-14101	112	27	master	master	NOUN
aiua-14101	112	28	mix	mix	NOUN
aiua-14101	112	29	green	green	NOUN
aiua-14101	112	30	(	(	PUNCT
aiua-14101	112	31	ampliqon	ampliqon	NOUN
aiua-14101	112	32	,	,	PUNCT
aiua-14101	112	33	denmark	denmark	NOUN
aiua-14101	112	34	)	)	PUNCT
aiua-14101	112	35	.	.	PUNCT
aiua-14101	113	1	gene	gene	NOUN
aiua-14101	113	2	-	-	PUNCT
aiua-14101	113	3	specific	specific	ADJ
aiua-14101	113	4	primers	primer	NOUN
aiua-14101	113	5	of	of	ADP
aiua-14101	113	6	bax	bax	PROPN
aiua-14101	113	7	,	,	PUNCT
aiua-14101	113	8	bcl-2	bcl-2	NOUN
aiua-14101	113	9	,	,	PUNCT
aiua-14101	113	10	and	and	CCONJ
aiua-14101	113	11	caspase-3	caspase-3	NUM
aiua-14101	113	12	,	,	PUNCT
aiua-14101	113	13	which	which	PRON
aiua-14101	113	14	involved	involve	VERB
aiua-14101	113	15	in	in	ADP
aiua-14101	113	16	the	the	DET
aiua-14101	113	17	apoptotic	apoptotic	ADJ
aiua-14101	113	18	pathway	pathway	NOUN
aiua-14101	113	19	,	,	PUNCT
aiua-14101	113	20	were	be	AUX
aiua-14101	113	21	used	use	VERB
aiua-14101	113	22	(	(	PUNCT
aiua-14101	113	23	table	table	NOUN
aiua-14101	113	24	1	1	NUM
aiua-14101	113	25	)	)	PUNCT
aiua-14101	113	26	.	.	PUNCT
aiua-14101	114	1	a	a	DET
aiua-14101	114	2	total	total	NOUN
aiua-14101	114	3	of	of	ADP
aiua-14101	114	4	100	100	NUM
aiua-14101	114	5	ng	ng	PROPN
aiua-14101	114	6	of	of	ADP
aiua-14101	114	7	cdna	cdna	PROPN
aiua-14101	114	8	was	be	AUX
aiua-14101	114	9	used	use	VERB
aiua-14101	114	10	per	per	ADP
aiua-14101	114	11	25	25	NUM
aiua-14101	114	12	μl	μl	NOUN
aiua-14101	114	13	reaction	reaction	NOUN
aiua-14101	114	14	,	,	PUNCT
aiua-14101	114	15	which	which	PRON
aiua-14101	114	16	included	include	VERB
aiua-14101	114	17	150	150	NUM
aiua-14101	114	18	nm	nm	NOUN
aiua-14101	114	19	of	of	ADP
aiua-14101	114	20	each	each	DET
aiua-14101	114	21	primer	primer	NOUN
aiua-14101	114	22	and	and	CCONJ
aiua-14101	114	23	12.5	12.5	NUM
aiua-14101	114	24	μl	μl	ADP
aiua-14101	114	25	of	of	ADP
aiua-14101	114	26	realq	realq	NOUN
aiua-14101	114	27	plus	plus	CCONJ
aiua-14101	114	28	master	master	NOUN
aiua-14101	114	29	mix	mix	NOUN
aiua-14101	114	30	.	.	PUNCT
aiua-14101	115	1	reactions	reaction	NOUN
aiua-14101	115	2	were	be	AUX
aiua-14101	115	3	run	run	VERB
aiua-14101	115	4	in	in	ADP
aiua-14101	115	5	a	a	DET
aiua-14101	115	6	cfx96	cfx96	ADJ
aiua-14101	115	7	system	system	NOUN
aiua-14101	115	8	(	(	PUNCT
aiua-14101	115	9	bio	bio	NOUN
aiua-14101	115	10	-	-	NOUN
aiua-14101	115	11	rad	rad	NOUN
aiua-14101	115	12	)	)	PUNCT
aiua-14101	115	13	with	with	ADP
aiua-14101	115	14	the	the	DET
aiua-14101	115	15	following	follow	VERB
aiua-14101	115	16	thermal	thermal	ADJ
aiua-14101	115	17	profile	profile	NOUN
aiua-14101	115	18	:	:	PUNCT
aiua-14101	115	19	initial	initial	ADJ
aiua-14101	115	20	denaturation	denaturation	NOUN
aiua-14101	115	21	at	at	ADP
aiua-14101	115	22	95	95	NUM
aiua-14101	115	23	°	°	ADP
aiua-14101	115	24	c	c	NOUN
aiua-14101	115	25	for	for	ADP
aiua-14101	115	26	15	15	NUM
aiua-14101	115	27	minutes	minute	NOUN
aiua-14101	115	28	,	,	PUNCT
aiua-14101	115	29	followed	follow	VERB
aiua-14101	115	30	by	by	ADP
aiua-14101	115	31	40	40	NUM
aiua-14101	115	32	cycles	cycle	NOUN
aiua-14101	115	33	of	of	ADP
aiua-14101	115	34	95	95	NUM
aiua-14101	115	35	°	°	ADP
aiua-14101	115	36	c	c	NOUN
aiua-14101	115	37	for	for	ADP
aiua-14101	115	38	30	30	NUM
aiua-14101	115	39	seconds	second	NOUN
aiua-14101	115	40	,	,	PUNCT
aiua-14101	115	41	60	60	NUM
aiua-14101	115	42	°	°	NUM
aiua-14101	115	43	c	c	NOUN
aiua-14101	115	44	for	for	ADP
aiua-14101	115	45	60	60	NUM
aiua-14101	115	46	seconds	second	NOUN
aiua-14101	115	47	,	,	PUNCT
aiua-14101	115	48	and	and	CCONJ
aiua-14101	115	49	72	72	NUM
aiua-14101	115	50	°	°	NOUN
aiua-14101	115	51	c	c	NOUN
aiua-14101	115	52	for	for	ADP
aiua-14101	115	53	30	30	NUM
aiua-14101	115	54	seconds	second	NOUN
aiua-14101	115	55	.	.	PUNCT
aiua-14101	116	1	gene	gene	NOUN
aiua-14101	116	2	expression	expression	NOUN
aiua-14101	116	3	levels	level	NOUN
aiua-14101	116	4	were	be	AUX
aiua-14101	116	5	normalized	normalize	VERB
aiua-14101	116	6	against	against	ADP
aiua-14101	116	7	housekeeping	housekeeping	NOUN
aiua-14101	116	8	gene	gene	NOUN
aiua-14101	116	9	(	(	PUNCT
aiua-14101	116	10	gapdh	gapdh	NOUN
aiua-14101	116	11	)	)	PUNCT
aiua-14101	116	12	using	use	VERB
aiua-14101	116	13	the	the	DET
aiua-14101	116	14	2δδct	2δδct	NUM
aiua-14101	116	15	method	method	NOUN
aiua-14101	116	16	(	(	PUNCT
aiua-14101	116	17	21	21	NUM
aiua-14101	116	18	)	)	PUNCT
aiua-14101	116	19	.	.	PUNCT
aiua-14101	117	1	data	datum	NOUN
aiua-14101	117	2	were	be	AUX
aiua-14101	117	3	reported	report	VERB
aiua-14101	117	4	as	as	ADP
aiua-14101	117	5	fold	fold	NOUN
aiua-14101	117	6	-	-	PUNCT
aiua-14101	117	7	change	change	NOUN
aiua-14101	117	8	,	,	PUNCT
aiua-14101	117	9	with	with	ADP
aiua-14101	117	10	experiments	experiment	NOUN
aiua-14101	117	11	conducted	conduct	VERB
aiua-14101	117	12	in	in	ADP
aiua-14101	117	13	two	two	NUM
aiua-14101	117	14	biological	biological	ADJ
aiua-14101	117	15	and	and	CCONJ
aiua-14101	117	16	technical	technical	ADJ
aiua-14101	117	17	replicates	replicate	NOUN
aiua-14101	117	18	.	.	PUNCT
aiua-14101	118	1	table	table	NOUN
aiua-14101	118	2	1	1	NUM
aiua-14101	118	3	.	.	PUNCT
aiua-14101	119	1	primer	primer	NOUN
aiua-14101	119	2	sets	set	NOUN
aiua-14101	119	3	used	use	VERB
aiua-14101	119	4	in	in	ADP
aiua-14101	119	5	the	the	DET
aiua-14101	119	6	study	study	NOUN
aiua-14101	119	7	.	.	PUNCT
aiua-14101	120	1	genes	gene	NOUN
aiua-14101	120	2	primer	primer	NOUN
aiua-14101	120	3	sequence	sequence	NOUN
aiua-14101	120	4	genbank	genbank	PROPN
aiua-14101	120	5	i	i	PROPN
aiua-14101	120	6	d	d	PROPN
aiua-14101	120	7	bax	bax	PROPN
aiua-14101	120	8	f	f	PROPN
aiua-14101	120	9	:	:	PUNCT
aiua-14101	120	10	5′-cacgtctgcggggagtc-3′	5′-cacgtctgcggggagtc-3′	NUM
aiua-14101	120	11	xm_063281064.1	xm_063281064.1	X
aiua-14101	121	1	r	r	X
aiua-14101	121	2	:	:	PUNCT
aiua-14101	121	3	5′-tgttgtccagttcatcgcca-3′	5′-tgttgtccagttcatcgcca-3′	NUM
aiua-14101	121	4	bcl-2	bcl-2	ADJ
aiua-14101	121	5	f	f	X
aiua-14101	121	6	:	:	PUNCT
aiua-14101	121	7	5′-gggctacgagtgggatactg-3′	5′-gggctacgagtgggatactg-3′	NUM
aiua-14101	121	8	nm_016993.2	nm_016993.2	NOUN
aiua-14101	121	9	r	r	NOUN
aiua-14101	121	10	:	:	PUNCT
aiua-14101	121	11	5′-gaccccaccgaactcaaaga-3′	5′-gaccccaccgaactcaaaga-3′	NUM
aiua-14101	121	12	caspase-3	caspase-3	NUM
aiua-14101	121	13	f	f	NOUN
aiua-14101	121	14	:	:	PUNCT
aiua-14101	121	15	5′-ggagcttggaacgcgaagaa-3′	5′-ggagcttggaacgcgaagaa-3′	NUM
aiua-14101	122	1	nm_012922.3	nm_012922.3	ADJ
aiua-14101	122	2	r	r	NOUN
aiua-14101	122	3	:	:	PUNCT
aiua-14101	122	4	5′-ccattgcgagctgacattcc-3′	5′-ccattgcgagctgacattcc-3′	NUM
aiua-14101	122	5	gapdh	gapdh	NOUN
aiua-14101	122	6	f	f	X
aiua-14101	122	7	:	:	PUNCT
aiua-14101	122	8	5′-ctctctgctcctccctgttc-3′	5′-ctctctgctcctccctgttc-3′	NUM
aiua-14101	122	9	nm_017008.4	nm_017008.4	VERB
aiua-14101	122	10	r	r	NOUN
aiua-14101	122	11	:	:	PUNCT
aiua-14101	122	12	5′-cgacatactcagcaccagca-3′	5′-cgacatactcagcaccagca-3′	PROPN
aiua-14101	122	13	bax	bax	PROPN
aiua-14101	122	14	,	,	PUNCT
aiua-14101	122	15	bcl-2	bcl-2	VERB
aiua-14101	122	16	associated	associate	VERB
aiua-14101	122	17	x	x	NOUN
aiua-14101	122	18	-	-	NOUN
aiua-14101	122	19	protein	protein	NOUN
aiua-14101	122	20	;	;	PUNCT
aiua-14101	122	21	bcl-2	bcl-2	NOUN
aiua-14101	122	22	,	,	PUNCT
aiua-14101	122	23	b	b	X
aiua-14101	122	24	-	-	PUNCT
aiua-14101	122	25	cell	cell	NOUN
aiua-14101	122	26	leukemia	leukemia	NOUN
aiua-14101	122	27	/	/	SYM
aiua-14101	122	28	lymphoma	lymphoma	NOUN
aiua-14101	122	29	2	2	NUM
aiua-14101	122	30	;	;	PUNCT
aiua-14101	122	31	gapdh	gapdh	NOUN
aiua-14101	122	32	,	,	PUNCT
aiua-14101	122	33	glyceraldehyde-3	glyceraldehyde-3	VERB
aiua-14101	122	34	-	-	ADJ
aiua-14101	122	35	phosphate	phosphate	ADJ
aiua-14101	122	36	dehydrogenase	dehydrogenase	NOUN
aiua-14101	122	37	.	.	PUNCT
aiua-14101	123	1	archivio	archivio	NOUN
aiua-14101	123	2	italiano	italiano	PROPN
aiua-14101	123	3	di	di	PROPN
aiua-14101	123	4	urologia	urologia	PROPN
aiua-14101	123	5	e	e	PROPN
aiua-14101	123	6	andrologia	andrologia	NOUN
aiua-14101	123	7	2025	2025	NUM
aiua-14101	123	8	;	;	PUNCT
aiua-14101	123	9	97(3):14101	97(3):14101	NUM
aiua-14101	123	10	f.	f.	PROPN
aiua-14101	123	11	yilmaz	yilmaz	PROPN
aiua-14101	123	12	dayanc	dayanc	PROPN
aiua-14101	123	13	,	,	PUNCT
aiua-14101	123	14	o.	o.	PROPN
aiua-14101	123	15	kaan	kaan	PROPN
aiua-14101	123	16	yalcin	yalcin	PROPN
aiua-14101	123	17	,	,	PUNCT
aiua-14101	123	18	a.	a.	NOUN
aiua-14101	123	19	eraslan	eraslan	PROPN
aiua-14101	123	20	sakar	sakar	PROPN
aiua-14101	123	21	4	4	NUM
aiua-14101	123	22	statistical	statistical	ADJ
aiua-14101	123	23	analysis	analysis	NOUN
aiua-14101	123	24	the	the	DET
aiua-14101	123	25	spss	spss	PROPN
aiua-14101	123	26	22.0	22.0	NUM
aiua-14101	123	27	software	software	NOUN
aiua-14101	123	28	was	be	AUX
aiua-14101	123	29	used	use	VERB
aiua-14101	123	30	for	for	ADP
aiua-14101	123	31	statistical	statistical	ADJ
aiua-14101	123	32	evaluations	evaluation	NOUN
aiua-14101	123	33	(	(	PUNCT
aiua-14101	123	34	ibm	ibm	PROPN
aiua-14101	123	35	corporation	corporation	PROPN
aiua-14101	123	36	,	,	PUNCT
aiua-14101	123	37	armonk	armonk	PROPN
aiua-14101	123	38	,	,	PUNCT
aiua-14101	123	39	ny	ny	PROPN
aiua-14101	123	40	)	)	PUNCT
aiua-14101	123	41	.	.	PUNCT
aiua-14101	124	1	in	in	ADP
aiua-14101	124	2	the	the	DET
aiua-14101	124	3	evaluation	evaluation	NOUN
aiua-14101	124	4	of	of	ADP
aiua-14101	124	5	spermatological	spermatological	ADJ
aiua-14101	124	6	parameters	parameter	NOUN
aiua-14101	124	7	,	,	PUNCT
aiua-14101	124	8	one	one	NUM
aiua-14101	124	9	-	-	PUNCT
aiua-14101	124	10	way	way	NOUN
aiua-14101	124	11	analysis	analysis	NOUN
aiua-14101	124	12	of	of	ADP
aiua-14101	124	13	variance	variance	NOUN
aiua-14101	124	14	(	(	PUNCT
aiua-14101	124	15	anova	anova	PROPN
aiua-14101	124	16	)	)	PUNCT
aiua-14101	124	17	were	be	AUX
aiua-14101	124	18	used	use	VERB
aiua-14101	124	19	to	to	PART
aiua-14101	124	20	compare	compare	VERB
aiua-14101	124	21	the	the	DET
aiua-14101	124	22	significance	significance	NOUN
aiua-14101	124	23	of	of	ADP
aiua-14101	124	24	the	the	DET
aiua-14101	124	25	difference	difference	NOUN
aiua-14101	124	26	between	between	ADP
aiua-14101	124	27	the	the	DET
aiua-14101	124	28	groups	group	NOUN
aiua-14101	124	29	.	.	PUNCT
aiua-14101	125	1	the	the	DET
aiua-14101	125	2	results	result	NOUN
aiua-14101	125	3	were	be	AUX
aiua-14101	125	4	evaluated	evaluate	VERB
aiua-14101	125	5	as	as	ADP
aiua-14101	125	6	mean	mean	NOUN
aiua-14101	125	7	±	±	NUM
aiua-14101	125	8	se	se	X
aiua-14101	125	9	and	and	CCONJ
aiua-14101	125	10	those	those	PRON
aiua-14101	125	11	with	with	ADP
aiua-14101	125	12	p	p	NOUN
aiua-14101	125	13	<	<	X
aiua-14101	125	14	0.05	0.05	NUM
aiua-14101	125	15	were	be	AUX
aiua-14101	125	16	considered	consider	VERB
aiua-14101	125	17	statistically	statistically	ADV
aiua-14101	125	18	significant	significant	ADJ
aiua-14101	125	19	.	.	PUNCT
aiua-14101	126	1	duncan	duncan	PROPN
aiua-14101	126	2	test	test	NOUN
aiua-14101	126	3	was	be	AUX
aiua-14101	126	4	used	use	VERB
aiua-14101	126	5	to	to	PART
aiua-14101	126	6	indicate	indicate	VERB
aiua-14101	126	7	the	the	DET
aiua-14101	126	8	significance	significance	NOUN
aiua-14101	126	9	of	of	ADP
aiua-14101	126	10	the	the	DET
aiua-14101	126	11	difference	difference	NOUN
aiua-14101	126	12	between	between	ADP
aiua-14101	126	13	the	the	DET
aiua-14101	126	14	groups	group	NOUN
aiua-14101	126	15	as	as	ADP
aiua-14101	126	16	a	a	DET
aiua-14101	126	17	result	result	NOUN
aiua-14101	126	18	of	of	ADP
aiua-14101	126	19	the	the	DET
aiua-14101	126	20	analysis	analysis	NOUN
aiua-14101	126	21	.	.	PUNCT
aiua-14101	127	1	for	for	ADP
aiua-14101	127	2	the	the	DET
aiua-14101	127	3	gene	gene	NOUN
aiua-14101	127	4	expression	expression	NOUN
aiua-14101	127	5	analysis	analysis	NOUN
aiua-14101	127	6	,	,	PUNCT
aiua-14101	127	7	a	a	DET
aiua-14101	127	8	one	one	NUM
aiua-14101	127	9	-	-	PUNCT
aiua-14101	127	10	way	way	NOUN
aiua-14101	127	11	anova	anova	PROPN
aiua-14101	127	12	followed	follow	VERB
aiua-14101	127	13	by	by	ADP
aiua-14101	127	14	a	a	DET
aiua-14101	127	15	post	post	X
aiua-14101	127	16	hoc	hoc	X
aiua-14101	127	17	tukey	tukey	NOUN
aiua-14101	127	18	test	test	NOUN
aiua-14101	127	19	was	be	AUX
aiua-14101	127	20	performed	perform	VERB
aiua-14101	127	21	using	use	VERB
aiua-14101	127	22	graphpad	graphpad	NOUN
aiua-14101	127	23	prism	prism	NOUN
aiua-14101	127	24	9.1.1	9.1.1	NUM
aiua-14101	127	25	(	(	PUNCT
aiua-14101	127	26	graphpad	graphpad	NOUN
aiua-14101	127	27	,	,	PUNCT
aiua-14101	127	28	san	san	PROPN
aiua-14101	127	29	diego	diego	PROPN
aiua-14101	127	30	,	,	PUNCT
aiua-14101	127	31	ca	ca	PROPN
aiua-14101	127	32	,	,	PUNCT
aiua-14101	127	33	usa	usa	PROPN
aiua-14101	127	34	)	)	PUNCT
aiua-14101	127	35	.	.	PUNCT
aiua-14101	128	1	statistical	statistical	ADJ
aiua-14101	128	2	significance	significance	NOUN
aiua-14101	128	3	was	be	AUX
aiua-14101	128	4	considered	consider	VERB
aiua-14101	128	5	at	at	ADP
aiua-14101	128	6	p	p	NOUN
aiua-14101	128	7	<	<	X
aiua-14101	128	8	0.05	0.05	NUM
aiua-14101	128	9	.	.	PUNCT
aiua-14101	129	1	results	result	VERB
aiua-14101	129	2	spermatologic	spermatologic	ADJ
aiua-14101	129	3	parameters	parameter	NOUN
aiua-14101	129	4	identified	identify	VERB
aiua-14101	129	5	after	after	ADP
aiua-14101	129	6	freezing	freeze	VERB
aiua-14101	129	7	-	-	PUNCT
aiua-14101	129	8	thawing	thaw	VERB
aiua-14101	129	9	the	the	DET
aiua-14101	129	10	motility	motility	NOUN
aiua-14101	129	11	,	,	PUNCT
aiua-14101	129	12	viability	viability	NOUN
aiua-14101	129	13	rate	rate	NOUN
aiua-14101	129	14	,	,	PUNCT
aiua-14101	129	15	plasma	plasma	NOUN
aiua-14101	129	16	membrane	membrane	NOUN
aiua-14101	129	17	integrity	integrity	NOUN
aiua-14101	129	18	rates	rate	NOUN
aiua-14101	129	19	,	,	PUNCT
aiua-14101	129	20	sperm	sperm	NOUN
aiua-14101	129	21	concentration	concentration	NOUN
aiua-14101	129	22	,	,	PUNCT
aiua-14101	129	23	and	and	CCONJ
aiua-14101	129	24	abnormal	abnormal	ADJ
aiua-14101	129	25	acrosome	acrosome	NOUN
aiua-14101	129	26	ratio	ratio	NOUN
aiua-14101	129	27	,	,	PUNCT
aiua-14101	129	28	in	in	ADP
aiua-14101	129	29	the	the	DET
aiua-14101	129	30	study	study	NOUN
aiua-14101	129	31	groups	group	NOUN
aiua-14101	129	32	after	after	SCONJ
aiua-14101	129	33	post	post	ADJ
aiua-14101	129	34	-	-	ADJ
aiua-14101	129	35	thawing	thaw	VERB
aiua-14101	129	36	processes	process	NOUN
aiua-14101	129	37	are	be	AUX
aiua-14101	129	38	presented	present	VERB
aiua-14101	129	39	in	in	ADP
aiua-14101	129	40	table	table	NOUN
aiua-14101	129	41	2	2	NUM
aiua-14101	129	42	.	.	X
aiua-14101	129	43	motility	motility	NOUN
aiua-14101	129	44	the	the	DET
aiua-14101	129	45	highest	high	ADJ
aiua-14101	129	46	motility	motility	NOUN
aiua-14101	129	47	ratio	ratio	NOUN
aiua-14101	129	48	was	be	AUX
aiua-14101	129	49	9.75	9.75	NUM
aiua-14101	129	50	±	±	NUM
aiua-14101	129	51	1.33	1.33	NUM
aiua-14101	129	52	in	in	ADP
aiua-14101	129	53	the	the	DET
aiua-14101	129	54	control	control	NOUN
aiua-14101	129	55	group	group	NOUN
aiua-14101	129	56	.	.	PUNCT
aiua-14101	130	1	this	this	DET
aiua-14101	130	2	ratio	ratio	NOUN
aiua-14101	130	3	was	be	AUX
aiua-14101	130	4	3.12	3.12	NUM
aiua-14101	130	5	±	±	NUM
aiua-14101	130	6	1.61	1.61	NUM
aiua-14101	130	7	in	in	ADP
aiua-14101	130	8	the	the	DET
aiua-14101	130	9	tryptophan	tryptophan	NOUN
aiua-14101	130	10	group	group	NOUN
aiua-14101	130	11	,	,	PUNCT
aiua-14101	130	12	which	which	PRON
aiua-14101	130	13	was	be	AUX
aiua-14101	130	14	significantly	significantly	ADV
aiua-14101	130	15	lower	low	ADJ
aiua-14101	130	16	compared	compare	VERB
aiua-14101	130	17	to	to	ADP
aiua-14101	130	18	the	the	DET
aiua-14101	130	19	control	control	NOUN
aiua-14101	130	20	group	group	NOUN
aiua-14101	130	21	(	(	PUNCT
aiua-14101	130	22	p	p	X
aiua-14101	130	23	<	<	X
aiua-14101	130	24	0.05	0.05	NUM
aiua-14101	130	25	)	)	PUNCT
aiua-14101	130	26	.	.	PUNCT
aiua-14101	131	1	in	in	ADP
aiua-14101	131	2	the	the	DET
aiua-14101	131	3	group	group	NOUN
aiua-14101	132	1	to	to	PART
aiua-14101	132	2	which	which	PRON
aiua-14101	132	3	100	100	NUM
aiua-14101	132	4	mm	mm	NOUN
aiua-14101	132	5	trehalose	trehalose	INTJ
aiua-14101	132	6	was	be	AUX
aiua-14101	132	7	added	add	VERB
aiua-14101	132	8	,	,	PUNCT
aiua-14101	132	9	the	the	DET
aiua-14101	132	10	motility	motility	NOUN
aiua-14101	132	11	ratio	ratio	NOUN
aiua-14101	132	12	was	be	AUX
aiua-14101	132	13	6.87	6.87	NUM
aiua-14101	132	14	±	±	NUM
aiua-14101	132	15	2.48	2.48	NUM
aiua-14101	132	16	,	,	PUNCT
aiua-14101	132	17	but	but	CCONJ
aiua-14101	132	18	there	there	PRON
aiua-14101	132	19	was	be	VERB
aiua-14101	132	20	no	no	DET
aiua-14101	132	21	statistically	statistically	ADV
aiua-14101	132	22	significant	significant	ADJ
aiua-14101	132	23	difference	difference	NOUN
aiua-14101	132	24	between	between	ADP
aiua-14101	132	25	the	the	DET
aiua-14101	132	26	groups	group	NOUN
aiua-14101	132	27	(	(	PUNCT
aiua-14101	132	28	p	p	X
aiua-14101	132	29	>	>	X
aiua-14101	132	30	0.05	0.05	NUM
aiua-14101	132	31	)	)	PUNCT
aiua-14101	132	32	.	.	PUNCT
aiua-14101	133	1	this	this	PRON
aiua-14101	133	2	indicates	indicate	VERB
aiua-14101	133	3	that	that	SCONJ
aiua-14101	133	4	tryptophan	tryptophan	NOUN
aiua-14101	133	5	and	and	CCONJ
aiua-14101	133	6	trehalose	trehalose	NOUN
aiua-14101	133	7	supplemented	supplement	VERB
aiua-14101	133	8	to	to	ADP
aiua-14101	133	9	the	the	DET
aiua-14101	133	10	cryopreservation	cryopreservation	NOUN
aiua-14101	133	11	diluent	diluent	NOUN
aiua-14101	133	12	may	may	AUX
aiua-14101	133	13	not	not	PART
aiua-14101	133	14	be	be	AUX
aiua-14101	133	15	effective	effective	ADJ
aiua-14101	133	16	on	on	ADP
aiua-14101	133	17	motility	motility	NOUN
aiua-14101	133	18	.	.	PUNCT
aiua-14101	134	1	viable	viable	ADJ
aiua-14101	134	2	spermatozoon	spermatozoon	NOUN
aiua-14101	134	3	ratio	ratio	NOUN
aiua-14101	134	4	there	there	PRON
aiua-14101	134	5	were	be	VERB
aiua-14101	134	6	significant	significant	ADJ
aiua-14101	134	7	differences	difference	NOUN
aiua-14101	134	8	between	between	ADP
aiua-14101	134	9	the	the	DET
aiua-14101	134	10	groups	group	NOUN
aiua-14101	134	11	in	in	ADP
aiua-14101	134	12	terms	term	NOUN
aiua-14101	134	13	of	of	ADP
aiua-14101	134	14	sperm	sperm	NOUN
aiua-14101	134	15	viability	viability	NOUN
aiua-14101	134	16	ratio	ratio	NOUN
aiua-14101	134	17	(	(	PUNCT
aiua-14101	134	18	p	p	X
aiua-14101	134	19	<	<	X
aiua-14101	134	20	0.05	0.05	NUM
aiua-14101	134	21	)	)	PUNCT
aiua-14101	134	22	.	.	PUNCT
aiua-14101	135	1	the	the	DET
aiua-14101	135	2	highest	high	ADJ
aiua-14101	135	3	viability	viability	NOUN
aiua-14101	135	4	ratio	ratio	NOUN
aiua-14101	135	5	was	be	AUX
aiua-14101	135	6	26.25	26.25	NUM
aiua-14101	135	7	±	±	NUM
aiua-14101	135	8	3.10	3.10	NUM
aiua-14101	135	9	in	in	ADP
aiua-14101	135	10	the	the	DET
aiua-14101	135	11	trehalose	trehalose	PROPN
aiua-14101	135	12	group	group	NOUN
aiua-14101	135	13	,	,	PUNCT
aiua-14101	135	14	which	which	PRON
aiua-14101	135	15	was	be	AUX
aiua-14101	135	16	significantly	significantly	ADV
aiua-14101	135	17	higher	high	ADJ
aiua-14101	135	18	than	than	ADP
aiua-14101	135	19	the	the	DET
aiua-14101	135	20	ratio	ratio	NOUN
aiua-14101	135	21	of	of	ADP
aiua-14101	135	22	19.62	19.62	NUM
aiua-14101	135	23	±	±	NUM
aiua-14101	135	24	1.51	1.51	NUM
aiua-14101	135	25	in	in	ADP
aiua-14101	135	26	the	the	DET
aiua-14101	135	27	control	control	NOUN
aiua-14101	135	28	group	group	NOUN
aiua-14101	135	29	.	.	PUNCT
aiua-14101	136	1	in	in	ADP
aiua-14101	136	2	the	the	DET
aiua-14101	136	3	group	group	NOUN
aiua-14101	136	4	in	in	ADP
aiua-14101	136	5	which	which	PRON
aiua-14101	136	6	25	25	NUM
aiua-14101	136	7	mm	mm	NOUN
aiua-14101	136	8	tryptophan	tryptophan	NOUN
aiua-14101	136	9	was	be	AUX
aiua-14101	136	10	added	add	VERB
aiua-14101	136	11	,	,	PUNCT
aiua-14101	136	12	the	the	DET
aiua-14101	136	13	viability	viability	NOUN
aiua-14101	136	14	ratio	ratio	NOUN
aiua-14101	136	15	was	be	AUX
aiua-14101	136	16	15.00	15.00	NUM
aiua-14101	136	17	±	±	NUM
aiua-14101	136	18	3.19	3.19	NUM
aiua-14101	136	19	,	,	PUNCT
aiua-14101	136	20	which	which	PRON
aiua-14101	136	21	was	be	AUX
aiua-14101	136	22	significantly	significantly	ADV
aiua-14101	136	23	lower	low	ADJ
aiua-14101	136	24	than	than	ADP
aiua-14101	136	25	the	the	DET
aiua-14101	136	26	ratios	ratio	NOUN
aiua-14101	136	27	in	in	ADP
aiua-14101	136	28	the	the	DET
aiua-14101	136	29	control	control	NOUN
aiua-14101	136	30	and	and	CCONJ
aiua-14101	136	31	trehalose	trehalose	ADJ
aiua-14101	136	32	groups	group	NOUN
aiua-14101	136	33	(	(	PUNCT
aiua-14101	136	34	p	p	X
aiua-14101	136	35	<	<	X
aiua-14101	136	36	0.05	0.05	NUM
aiua-14101	136	37	)	)	PUNCT
aiua-14101	136	38	.	.	PUNCT
aiua-14101	137	1	these	these	DET
aiua-14101	137	2	findings	finding	NOUN
aiua-14101	137	3	suggest	suggest	VERB
aiua-14101	137	4	that	that	SCONJ
aiua-14101	137	5	trehalose	trehalose	PRON
aiua-14101	137	6	may	may	AUX
aiua-14101	137	7	improve	improve	VERB
aiua-14101	137	8	sperm	sperm	NOUN
aiua-14101	137	9	viability	viability	NOUN
aiua-14101	137	10	after	after	ADP
aiua-14101	137	11	freezing	freeze	VERB
aiua-14101	137	12	-	-	PUNCT
aiua-14101	137	13	thawing	thaw	VERB
aiua-14101	137	14	.	.	PUNCT
aiua-14101	138	1	plasma	plasma	NOUN
aiua-14101	138	2	membrane	membrane	NOUN
aiua-14101	138	3	integrity	integrity	NOUN
aiua-14101	138	4	ratio	ratio	NOUN
aiua-14101	138	5	according	accord	VERB
aiua-14101	138	6	to	to	ADP
aiua-14101	138	7	the	the	DET
aiua-14101	138	8	results	result	NOUN
aiua-14101	138	9	of	of	ADP
aiua-14101	138	10	host	host	NOUN
aiua-14101	138	11	assay	assay	NOUN
aiua-14101	138	12	performed	perform	VERB
aiua-14101	138	13	to	to	PART
aiua-14101	138	14	evaluate	evaluate	VERB
aiua-14101	138	15	sperm	sperm	NOUN
aiua-14101	138	16	cell	cell	NOUN
aiua-14101	138	17	membrane	membrane	NOUN
aiua-14101	138	18	integrity	integrity	NOUN
aiua-14101	138	19	,	,	PUNCT
aiua-14101	138	20	there	there	PRON
aiua-14101	138	21	were	be	VERB
aiua-14101	138	22	significant	significant	ADJ
aiua-14101	138	23	differences	difference	NOUN
aiua-14101	138	24	between	between	ADP
aiua-14101	138	25	the	the	DET
aiua-14101	138	26	groups	group	NOUN
aiua-14101	138	27	(	(	PUNCT
aiua-14101	138	28	p	p	X
aiua-14101	138	29	<	<	X
aiua-14101	138	30	0.05	0.05	NUM
aiua-14101	138	31	)	)	PUNCT
aiua-14101	138	32	.	.	PUNCT
aiua-14101	139	1	the	the	DET
aiua-14101	139	2	highest	high	ADJ
aiua-14101	139	3	host	host	NOUN
aiua-14101	139	4	positive	positive	ADJ
aiua-14101	139	5	ratio	ratio	NOUN
aiua-14101	139	6	was	be	AUX
aiua-14101	139	7	determined	determine	VERB
aiua-14101	139	8	in	in	ADP
aiua-14101	139	9	the	the	DET
aiua-14101	139	10	control	control	NOUN
aiua-14101	139	11	group	group	NOUN
aiua-14101	139	12	with	with	ADP
aiua-14101	139	13	(	(	PUNCT
aiua-14101	139	14	22.87	22.87	NUM
aiua-14101	139	15	±	±	NUM
aiua-14101	139	16	1.10	1.10	NUM
aiua-14101	139	17	)	)	PUNCT
aiua-14101	139	18	and	and	CCONJ
aiua-14101	139	19	in	in	ADP
aiua-14101	139	20	the	the	DET
aiua-14101	139	21	trehalose	trehalose	PROPN
aiua-14101	139	22	group	group	NOUN
aiua-14101	139	23	(	(	PUNCT
aiua-14101	139	24	20.25	20.25	NUM
aiua-14101	139	25	±	±	NUM
aiua-14101	139	26	3.65	3.65	NUM
aiua-14101	139	27	)	)	PUNCT
aiua-14101	139	28	.	.	PUNCT
aiua-14101	140	1	there	there	PRON
aiua-14101	140	2	was	be	VERB
aiua-14101	140	3	no	no	DET
aiua-14101	140	4	statistical	statistical	ADJ
aiua-14101	140	5	difference	difference	NOUN
aiua-14101	140	6	between	between	ADP
aiua-14101	140	7	the	the	DET
aiua-14101	140	8	groups	group	NOUN
aiua-14101	140	9	(	(	PUNCT
aiua-14101	140	10	p	p	X
aiua-14101	140	11	>	>	X
aiua-14101	140	12	0.05	0.05	NUM
aiua-14101	140	13	)	)	PUNCT
aiua-14101	140	14	.	.	PUNCT
aiua-14101	141	1	the	the	DET
aiua-14101	141	2	host	host	NOUN
aiua-14101	141	3	positive	positive	ADJ
aiua-14101	141	4	ratio	ratio	NOUN
aiua-14101	141	5	was	be	AUX
aiua-14101	141	6	11.87	11.87	NUM
aiua-14101	141	7	±	±	NUM
aiua-14101	141	8	3.07	3.07	NUM
aiua-14101	141	9	in	in	ADP
aiua-14101	141	10	the	the	DET
aiua-14101	141	11	tryptophan	tryptophan	NOUN
aiua-14101	141	12	group	group	NOUN
aiua-14101	141	13	and	and	CCONJ
aiua-14101	141	14	significantly	significantly	ADV
aiua-14101	141	15	lower	lower	ADV
aiua-14101	141	16	compared	compare	VERB
aiua-14101	141	17	to	to	ADP
aiua-14101	141	18	the	the	DET
aiua-14101	141	19	control	control	NOUN
aiua-14101	141	20	group	group	NOUN
aiua-14101	141	21	(	(	PUNCT
aiua-14101	141	22	p	p	X
aiua-14101	141	23	<	<	X
aiua-14101	141	24	0.05	0.05	NUM
aiua-14101	141	25	)	)	PUNCT
aiua-14101	141	26	.	.	PUNCT
aiua-14101	142	1	spermatozoa	spermatozoa	NOUN
aiua-14101	142	2	density	density	NOUN
aiua-14101	142	3	spermatozoa	spermatozoa	NOUN
aiua-14101	142	4	density	density	NOUN
aiua-14101	142	5	was	be	AUX
aiua-14101	142	6	determined	determine	VERB
aiua-14101	142	7	to	to	PART
aiua-14101	142	8	verify	verify	VERB
aiua-14101	142	9	the	the	DET
aiua-14101	142	10	amount	amount	NOUN
aiua-14101	142	11	of	of	ADP
aiua-14101	142	12	cells	cell	NOUN
aiua-14101	142	13	required	require	VERB
aiua-14101	142	14	for	for	ADP
aiua-14101	142	15	analyses	analysis	NOUN
aiua-14101	142	16	to	to	PART
aiua-14101	142	17	be	be	AUX
aiua-14101	142	18	performed	perform	VERB
aiua-14101	142	19	after	after	ADP
aiua-14101	142	20	freezing	freeze	VERB
aiua-14101	142	21	-	-	PUNCT
aiua-14101	142	22	thawing	thaw	VERB
aiua-14101	142	23	and	and	CCONJ
aiua-14101	142	24	to	to	PART
aiua-14101	142	25	standardize	standardize	VERB
aiua-14101	142	26	experimental	experimental	ADJ
aiua-14101	142	27	procedures	procedure	NOUN
aiua-14101	142	28	.	.	PUNCT
aiua-14101	143	1	sperm	sperm	NOUN
aiua-14101	143	2	concentration	concentration	NOUN
aiua-14101	143	3	did	do	AUX
aiua-14101	143	4	not	not	PART
aiua-14101	143	5	differ	differ	VERB
aiua-14101	143	6	significantly	significantly	ADV
aiua-14101	143	7	among	among	ADP
aiua-14101	143	8	the	the	DET
aiua-14101	143	9	experimental	experimental	ADJ
aiua-14101	143	10	groups	group	NOUN
aiua-14101	143	11	(	(	PUNCT
aiua-14101	143	12	p	p	X
aiua-14101	143	13	>	>	X
aiua-14101	143	14	0.05	0.05	NUM
aiua-14101	143	15	)	)	PUNCT
aiua-14101	143	16	.	.	PUNCT
aiua-14101	144	1	abnormal	abnormal	ADJ
aiua-14101	144	2	acrosome	acrosome	NOUN
aiua-14101	144	3	ratio	ratio	NOUN
aiua-14101	144	4	there	there	PRON
aiua-14101	144	5	was	be	VERB
aiua-14101	144	6	no	no	DET
aiua-14101	144	7	statistically	statistically	ADV
aiua-14101	144	8	significant	significant	ADJ
aiua-14101	144	9	difference	difference	NOUN
aiua-14101	144	10	between	between	ADP
aiua-14101	144	11	the	the	DET
aiua-14101	144	12	groups	group	NOUN
aiua-14101	144	13	in	in	ADP
aiua-14101	144	14	terms	term	NOUN
aiua-14101	144	15	of	of	ADP
aiua-14101	144	16	abnormal	abnormal	ADJ
aiua-14101	144	17	acrosome	acrosome	NOUN
aiua-14101	144	18	ratio	ratio	NOUN
aiua-14101	144	19	(	(	PUNCT
aiua-14101	144	20	p	p	X
aiua-14101	144	21	>	>	X
aiua-14101	144	22	0.05	0.05	NUM
aiua-14101	144	23	)	)	PUNCT
aiua-14101	144	24	.	.	PUNCT
aiua-14101	145	1	the	the	DET
aiua-14101	145	2	abnormal	abnormal	ADJ
aiua-14101	145	3	acrosome	acrosome	NOUN
aiua-14101	145	4	ratio	ratio	NOUN
aiua-14101	145	5	was	be	AUX
aiua-14101	145	6	6.37	6.37	NUM
aiua-14101	145	7	±	±	NUM
aiua-14101	145	8	0.99	0.99	NUM
aiua-14101	145	9	in	in	ADP
aiua-14101	145	10	the	the	DET
aiua-14101	145	11	control	control	NOUN
aiua-14101	145	12	group	group	NOUN
aiua-14101	145	13	,	,	PUNCT
aiua-14101	145	14	4.75	4.75	NUM
aiua-14101	145	15	±	±	NUM
aiua-14101	145	16	0.75	0.75	NUM
aiua-14101	145	17	in	in	ADP
aiua-14101	145	18	the	the	DET
aiua-14101	145	19	tryptophan	tryptophan	NOUN
aiua-14101	145	20	group	group	NOUN
aiua-14101	145	21	,	,	PUNCT
aiua-14101	145	22	and	and	CCONJ
aiua-14101	145	23	4.87	4.87	NUM
aiua-14101	145	24	±	±	NUM
aiua-14101	145	25	0.63	0.63	NUM
aiua-14101	145	26	in	in	ADP
aiua-14101	145	27	the	the	DET
aiua-14101	145	28	trehalose	trehalose	PROPN
aiua-14101	145	29	group	group	NOUN
aiua-14101	145	30	.	.	PUNCT
aiua-14101	146	1	apoptotic	apoptotic	ADJ
aiua-14101	146	2	cell	cell	NOUN
aiua-14101	146	3	ratios	ratio	NOUN
aiua-14101	146	4	determined	determine	VERB
aiua-14101	146	5	with	with	ADP
aiua-14101	146	6	annexin	annexin	NOUN
aiua-14101	146	7	v	v	NOUN
aiua-14101	146	8	-	-	PUNCT
aiua-14101	146	9	fitc	fitc	NOUN
aiua-14101	146	10	/	/	SYM
aiua-14101	146	11	pi	pi	NOUN
aiua-14101	146	12	staining	stain	VERB
aiua-14101	146	13	the	the	DET
aiua-14101	146	14	apoptotic	apoptotic	ADJ
aiua-14101	146	15	cell	cell	NOUN
aiua-14101	146	16	ratios	ratio	NOUN
aiua-14101	146	17	determined	determine	VERB
aiua-14101	146	18	with	with	ADP
aiua-14101	146	19	annexin	annexin	NOUN
aiua-14101	146	20	vfitc	vfitc	NOUN
aiua-14101	146	21	and	and	CCONJ
aiua-14101	146	22	pi	pi	NOUN
aiua-14101	146	23	staining	stain	VERB
aiua-14101	146	24	are	be	AUX
aiua-14101	146	25	presented	present	VERB
aiua-14101	146	26	in	in	ADP
aiua-14101	146	27	figure	figure	NOUN
aiua-14101	146	28	1	1	NUM
aiua-14101	146	29	.	.	PUNCT
aiua-14101	146	30	accordingly	accordingly	ADV
aiua-14101	146	31	,	,	PUNCT
aiua-14101	146	32	the	the	DET
aiua-14101	146	33	lowest	low	ADJ
aiua-14101	146	34	ratio	ratio	NOUN
aiua-14101	146	35	of	of	ADP
aiua-14101	146	36	apoptotic	apoptotic	ADJ
aiua-14101	146	37	cells	cell	NOUN
aiua-14101	146	38	was	be	AUX
aiua-14101	146	39	determined	determine	VERB
aiua-14101	146	40	in	in	ADP
aiua-14101	146	41	the	the	DET
aiua-14101	146	42	group	group	NOUN
aiua-14101	146	43	in	in	ADP
aiua-14101	146	44	which	which	PRON
aiua-14101	146	45	100	100	NUM
aiua-14101	146	46	mm	mm	NOUN
aiua-14101	146	47	trehalose	trehalose	INTJ
aiua-14101	146	48	was	be	AUX
aiua-14101	146	49	added	add	VERB
aiua-14101	146	50	(	(	PUNCT
aiua-14101	146	51	22.37	22.37	NUM
aiua-14101	146	52	±	±	NUM
aiua-14101	146	53	2.94	2.94	NUM
aiua-14101	146	54	)	)	PUNCT
aiua-14101	146	55	;	;	PUNCT
aiua-14101	146	56	however	however	ADV
aiua-14101	146	57	,	,	PUNCT
aiua-14101	146	58	this	this	DET
aiua-14101	146	59	value	value	NOUN
aiua-14101	146	60	was	be	AUX
aiua-14101	146	61	not	not	PART
aiua-14101	146	62	significantly	significantly	ADV
aiua-14101	146	63	different	different	ADJ
aiua-14101	146	64	from	from	ADP
aiua-14101	146	65	the	the	DET
aiua-14101	146	66	ratio	ratio	NOUN
aiua-14101	146	67	of	of	ADP
aiua-14101	146	68	33.00	33.00	NUM
aiua-14101	146	69	±	±	NUM
aiua-14101	146	70	4.55	4.55	NUM
aiua-14101	146	71	determined	determine	VERB
aiua-14101	146	72	in	in	ADP
aiua-14101	146	73	the	the	DET
aiua-14101	146	74	control	control	NOUN
aiua-14101	146	75	group	group	NOUN
aiua-14101	146	76	(	(	PUNCT
aiua-14101	146	77	p	p	X
aiua-14101	146	78	>	>	X
aiua-14101	146	79	0.05	0.05	NUM
aiua-14101	146	80	)	)	PUNCT
aiua-14101	146	81	.	.	PUNCT
aiua-14101	147	1	the	the	DET
aiua-14101	147	2	highest	high	ADJ
aiua-14101	147	3	ratio	ratio	NOUN
aiua-14101	147	4	was	be	AUX
aiua-14101	147	5	determined	determine	VERB
aiua-14101	147	6	as	as	ADP
aiua-14101	147	7	55.00	55.00	NUM
aiua-14101	147	8	±	±	NUM
aiua-14101	147	9	3.95	3.95	NUM
aiua-14101	147	10	in	in	ADP
aiua-14101	147	11	the	the	DET
aiua-14101	147	12	tryptophan	tryptophan	NOUN
aiua-14101	147	13	group	group	NOUN
aiua-14101	147	14	,	,	PUNCT
aiua-14101	147	15	which	which	PRON
aiua-14101	147	16	was	be	AUX
aiua-14101	147	17	significantly	significantly	ADV
aiua-14101	147	18	higher	high	ADJ
aiua-14101	147	19	than	than	ADP
aiua-14101	147	20	that	that	PRON
aiua-14101	147	21	in	in	ADP
aiua-14101	147	22	the	the	DET
aiua-14101	147	23	control	control	NOUN
aiua-14101	147	24	and	and	CCONJ
aiua-14101	147	25	trehalose	trehalose	ADJ
aiua-14101	147	26	groups	group	NOUN
aiua-14101	147	27	(	(	PUNCT
aiua-14101	147	28	p	p	X
aiua-14101	147	29	<	<	X
aiua-14101	147	30	0.01	0.01	NUM
aiua-14101	147	31	)	)	PUNCT
aiua-14101	147	32	.	.	PUNCT
aiua-14101	148	1	effects	effect	NOUN
aiua-14101	148	2	of	of	ADP
aiua-14101	148	3	tryptophan	tryptophan	NOUN
aiua-14101	148	4	and	and	CCONJ
aiua-14101	148	5	trehalose	trehalose	ADJ
aiua-14101	148	6	supplementation	supplementation	NOUN
aiua-14101	148	7	on	on	ADP
aiua-14101	148	8	the	the	DET
aiua-14101	148	9	expression	expression	NOUN
aiua-14101	148	10	of	of	ADP
aiua-14101	148	11	apoptosis	apoptosis	NOUN
aiua-14101	148	12	-	-	PUNCT
aiua-14101	148	13	related	relate	VERB
aiua-14101	148	14	genes	gene	NOUN
aiua-14101	148	15	in	in	ADP
aiua-14101	148	16	frozen	frozen	ADJ
aiua-14101	148	17	-	-	PUNCT
aiua-14101	148	18	thawed	thaw	VERB
aiua-14101	148	19	sperm	sperm	NOUN
aiua-14101	148	20	using	use	VERB
aiua-14101	148	21	real	real	ADJ
aiua-14101	148	22	-	-	PUNCT
aiua-14101	148	23	time	time	NOUN
aiua-14101	148	24	pcr	pcr	NOUN
aiua-14101	148	25	to	to	PART
aiua-14101	148	26	investigate	investigate	VERB
aiua-14101	148	27	whether	whether	SCONJ
aiua-14101	148	28	tryptophan	tryptophan	NOUN
aiua-14101	148	29	and	and	CCONJ
aiua-14101	148	30	trehalose	trehalose	PRON
aiua-14101	148	31	can	can	AUX
aiua-14101	148	32	alletable	alletable	VERB
aiua-14101	148	33	2	2	NUM
aiua-14101	148	34	.	.	PUNCT
aiua-14101	149	1	spermatological	spermatological	ADJ
aiua-14101	149	2	findings	finding	NOUN
aiua-14101	149	3	determined	determine	VERB
aiua-14101	149	4	after	after	ADP
aiua-14101	149	5	freezing	freeze	VERB
aiua-14101	149	6	-	-	PUNCT
aiua-14101	149	7	thawing	thawing	NOUN
aiua-14101	149	8	in	in	ADP
aiua-14101	149	9	the	the	DET
aiua-14101	149	10	study	study	NOUN
aiua-14101	149	11	groups	group	NOUN
aiua-14101	149	12	(	(	PUNCT
aiua-14101	149	13	n	n	NOUN
aiua-14101	149	14	=	=	SYM
aiua-14101	149	15	8)	8)	NUM
aiua-14101	149	16	.	.	PUNCT
aiua-14101	150	1	groups	group	NOUN
aiua-14101	150	2	progressive	progressive	ADJ
aiua-14101	150	3	motility	motility	NOUN
aiua-14101	150	4	viability	viability	NOUN
aiua-14101	150	5	intact	intact	ADJ
aiua-14101	150	6	plasma	plasma	NOUN
aiua-14101	150	7	sperm	sperm	NOUN
aiua-14101	150	8	concentration	concentration	NOUN
aiua-14101	150	9	abnormal	abnormal	ADJ
aiua-14101	150	10	acrosome	acrosome	NOUN
aiua-14101	150	11	(	(	PUNCT
aiua-14101	150	12	%	%	NOUN
aiua-14101	150	13	)	)	PUNCT
aiua-14101	150	14	(	(	PUNCT
aiua-14101	150	15	%	%	INTJ
aiua-14101	150	16	)	)	PUNCT
aiua-14101	150	17	membrane	membrane	NOUN
aiua-14101	150	18	integrity	integrity	NOUN
aiua-14101	150	19	(	(	PUNCT
aiua-14101	150	20	%	%	INTJ
aiua-14101	150	21	)	)	PUNCT
aiua-14101	150	22	(	(	PUNCT
aiua-14101	150	23	×10⁶	×10⁶	NOUN
aiua-14101	150	24	cells	cell	NOUN
aiua-14101	150	25	/	/	SYM
aiua-14101	150	26	ml	ml	NOUN
aiua-14101	150	27	)	)	PUNCT
aiua-14101	150	28	(	(	PUNCT
aiua-14101	150	29	%	%	INTJ
aiua-14101	150	30	)	)	PUNCT
aiua-14101	150	31	control	control	NOUN
aiua-14101	150	32	(	(	PUNCT
aiua-14101	150	33	c	c	NOUN
aiua-14101	150	34	)	)	PUNCT
aiua-14101	150	35	9.75	9.75	NUM
aiua-14101	150	36	±	±	NUM
aiua-14101	150	37	1.33	1.33	NUM
aiua-14101	150	38	19.62	19.62	NUM
aiua-14101	150	39	±	±	NUM
aiua-14101	150	40	1.51	1.51	NUM
aiua-14101	150	41	22.87	22.87	NUM
aiua-14101	150	42	±	±	NUM
aiua-14101	150	43	1.10	1.10	NUM
aiua-14101	150	44	221.00	221.00	NUM
aiua-14101	150	45	±	±	NUM
aiua-14101	150	46	3.40	3.40	NUM
aiua-14101	150	47	6.37	6.37	NUM
aiua-14101	150	48	±	±	NUM
aiua-14101	150	49	0.99	0.99	NUM
aiua-14101	150	50	tryptophan	tryptophan	NOUN
aiua-14101	150	51	(	(	PUNCT
aiua-14101	150	52	tryp	tryp	PROPN
aiua-14101	150	53	)	)	PUNCT
aiua-14101	150	54	(	(	PUNCT
aiua-14101	150	55	25	25	NUM
aiua-14101	150	56	mm	mm	NOUN
aiua-14101	150	57	)	)	PUNCT
aiua-14101	150	58	3.12	3.12	NUM
aiua-14101	150	59	±	±	NUM
aiua-14101	150	60	1.61	1.61	NUM
aiua-14101	150	61	15.00	15.00	NUM
aiua-14101	150	62	±	±	NUM
aiua-14101	150	63	3.19	3.19	NUM
aiua-14101	150	64	11.87	11.87	NUM
aiua-14101	150	65	±	±	NUM
aiua-14101	150	66	3.07	3.07	NUM
aiua-14101	150	67	245.75	245.75	NUM
aiua-14101	150	68	±	±	NUM
aiua-14101	150	69	5.74	5.74	NUM
aiua-14101	150	70	4.75	4.75	NUM
aiua-14101	150	71	±	±	NUM
aiua-14101	150	72	0.75	0.75	NUM
aiua-14101	150	73	trehalose	trehalose	INTJ
aiua-14101	150	74	(	(	PUNCT
aiua-14101	150	75	treh	treh	NOUN
aiua-14101	150	76	)	)	PUNCT
aiua-14101	150	77	(	(	PUNCT
aiua-14101	150	78	100	100	NUM
aiua-14101	150	79	mm	mm	NOUN
aiua-14101	150	80	)	)	PUNCT
aiua-14101	150	81	6.87	6.87	NUM
aiua-14101	150	82	±	±	NUM
aiua-14101	150	83	2.48	2.48	NUM
aiua-14101	150	84	26.25	26.25	NUM
aiua-14101	150	85	±	±	NUM
aiua-14101	150	86	3.10	3.10	NUM
aiua-14101	150	87	20.25	20.25	NUM
aiua-14101	150	88	±	±	NUM
aiua-14101	150	89	3.65	3.65	NUM
aiua-14101	150	90	245.5	245.5	NUM
aiua-14101	150	91	±	±	NUM
aiua-14101	150	92	4.25	4.25	NUM
aiua-14101	150	93	4.87	4.87	NUM
aiua-14101	150	94	±	±	NUM
aiua-14101	150	95	0.63	0.63	NUM
aiua-14101	150	96	c	c	NOUN
aiua-14101	150	97	vs	vs	ADP
aiua-14101	150	98	tryp	tryp	PROPN
aiua-14101	150	99	p	p	X
aiua-14101	150	100	<	<	X
aiua-14101	150	101	0.05	0.05	NUM
aiua-14101	150	102	p	p	NOUN
aiua-14101	150	103	<	<	X
aiua-14101	150	104	0.05	0.05	NUM
aiua-14101	150	105	p	p	NOUN
aiua-14101	150	106	<	<	X
aiua-14101	150	107	0.05	0.05	NUM
aiua-14101	150	108	p	p	NOUN
aiua-14101	150	109	<	<	X
aiua-14101	150	110	0.05	0.05	NUM
aiua-14101	150	111	p	p	NOUN
aiua-14101	150	112	<	<	X
aiua-14101	150	113	0.05	0.05	NUM
aiua-14101	150	114	c	c	NOUN
aiua-14101	150	115	vs	vs	ADP
aiua-14101	150	116	treh	treh	NOUN
aiua-14101	150	117	p	p	X
aiua-14101	150	118	<	<	X
aiua-14101	150	119	0.05	0.05	NUM
aiua-14101	150	120	p	p	NOUN
aiua-14101	150	121	<	<	X
aiua-14101	150	122	0.05	0.05	NUM
aiua-14101	150	123	p	p	NOUN
aiua-14101	150	124	<	<	X
aiua-14101	150	125	0.05	0.05	NUM
aiua-14101	150	126	p	p	NOUN
aiua-14101	150	127	<	<	X
aiua-14101	150	128	0.05	0.05	NUM
aiua-14101	150	129	p	p	NOUN
aiua-14101	150	130	<	<	X
aiua-14101	150	131	0.05	0.05	NUM
aiua-14101	150	132	tryp	tryp	NOUN
aiua-14101	150	133	vs	vs	ADP
aiua-14101	150	134	treh	treh	PRON
aiua-14101	150	135	p	p	X
aiua-14101	150	136	<	<	X
aiua-14101	150	137	0.05	0.05	NUM
aiua-14101	150	138	p	p	NOUN
aiua-14101	150	139	<	<	X
aiua-14101	150	140	0.05	0.05	NUM
aiua-14101	150	141	p	p	NOUN
aiua-14101	150	142	<	<	X
aiua-14101	150	143	0.05	0.05	NUM
aiua-14101	150	144	p	p	NOUN
aiua-14101	150	145	<	<	X
aiua-14101	150	146	0.05	0.05	NUM
aiua-14101	150	147	p	p	NOUN
aiua-14101	150	148	<	<	X
aiua-14101	150	149	0.05	0.05	NUM
aiua-14101	150	150	archivio	archivio	NOUN
aiua-14101	150	151	italiano	italiano	NOUN
aiua-14101	150	152	di	di	X
aiua-14101	150	153	urologia	urologia	PROPN
aiua-14101	150	154	e	e	PROPN
aiua-14101	150	155	andrologia	andrologia	NOUN
aiua-14101	150	156	2025	2025	NUM
aiua-14101	150	157	;	;	PUNCT
aiua-14101	150	158	97(3):14101	97(3):14101	NUM
aiua-14101	150	159	5	5	NUM
aiua-14101	150	160	tryptophan	tryptophan	NOUN
aiua-14101	150	161	and	and	CCONJ
aiua-14101	150	162	trehalose	trehalose	NOUN
aiua-14101	150	163	on	on	ADP
aiua-14101	150	164	post	post	ADJ
aiua-14101	150	165	-	-	ADJ
aiua-14101	150	166	thaw	thaw	ADJ
aiua-14101	150	167	sperm	sperm	NOUN
aiua-14101	150	168	viate	viate	NOUN
aiua-14101	150	169	cryodamage	cryodamage	NOUN
aiua-14101	150	170	in	in	ADP
aiua-14101	150	171	spermatozoa	spermatozoon	NOUN
aiua-14101	150	172	after	after	ADP
aiua-14101	150	173	frozen	freeze	VERB
aiua-14101	150	174	-	-	PUNCT
aiua-14101	150	175	thawed	thaw	VERB
aiua-14101	150	176	were	be	AUX
aiua-14101	150	177	analyzed	analyze	VERB
aiua-14101	150	178	the	the	DET
aiua-14101	150	179	expression	expression	NOUN
aiua-14101	150	180	of	of	ADP
aiua-14101	150	181	apoptosis	apoptosis	NOUN
aiua-14101	150	182	-	-	PUNCT
aiua-14101	150	183	related	relate	VERB
aiua-14101	150	184	genes	gene	NOUN
aiua-14101	150	185	.	.	PUNCT
aiua-14101	151	1	addition	addition	NOUN
aiua-14101	151	2	of	of	ADP
aiua-14101	151	3	25	25	NUM
aiua-14101	151	4	mm	mm	NOUN
aiua-14101	151	5	tryptophan	tryptophan	NOUN
aiua-14101	151	6	and	and	CCONJ
aiua-14101	151	7	100	100	NUM
aiua-14101	151	8	mm	mm	NOUN
aiua-14101	151	9	trehalose	trehalose	INTJ
aiua-14101	151	10	to	to	ADP
aiua-14101	151	11	the	the	DET
aiua-14101	151	12	cryoprotectant	cryoprotectant	NOUN
aiua-14101	151	13	agent	agent	NOUN
aiua-14101	151	14	affected	affect	VERB
aiua-14101	151	15	bax	bax	PROPN
aiua-14101	151	16	,	,	PUNCT
aiua-14101	151	17	bcl-2	bcl-2	NOUN
aiua-14101	151	18	,	,	PUNCT
aiua-14101	151	19	and	and	CCONJ
aiua-14101	151	20	caspase3	caspase3	PROPN
aiua-14101	151	21	mrna	mrna	PROPN
aiua-14101	151	22	levels	level	NOUN
aiua-14101	151	23	in	in	ADP
aiua-14101	151	24	comparison	comparison	NOUN
aiua-14101	151	25	to	to	ADP
aiua-14101	151	26	the	the	DET
aiua-14101	151	27	control	control	NOUN
aiua-14101	151	28	group	group	NOUN
aiua-14101	151	29	(	(	PUNCT
aiua-14101	151	30	figure	figure	NOUN
aiua-14101	151	31	2	2	NUM
aiua-14101	151	32	)	)	PUNCT
aiua-14101	151	33	.	.	PUNCT
aiua-14101	152	1	bax	bax	NOUN
aiua-14101	152	2	gene	gene	NOUN
aiua-14101	152	3	expression	expression	NOUN
aiua-14101	152	4	was	be	AUX
aiua-14101	152	5	significantly	significantly	ADV
aiua-14101	152	6	downregulated	downregulate	VERB
aiua-14101	152	7	in	in	ADP
aiua-14101	152	8	both	both	CCONJ
aiua-14101	152	9	the	the	DET
aiua-14101	152	10	tryptophan	tryptophan	NOUN
aiua-14101	152	11	and	and	CCONJ
aiua-14101	152	12	trehalose	trehalose	ADV
aiua-14101	152	13	-	-	PUNCT
aiua-14101	152	14	treated	treat	VERB
aiua-14101	152	15	groups	group	NOUN
aiua-14101	152	16	compared	compare	VERB
aiua-14101	152	17	to	to	ADP
aiua-14101	152	18	the	the	DET
aiua-14101	152	19	control	control	NOUN
aiua-14101	152	20	group	group	NOUN
aiua-14101	152	21	(	(	PUNCT
aiua-14101	152	22	p	p	X
aiua-14101	152	23	<	<	X
aiua-14101	152	24	0.001	0.001	NUM
aiua-14101	152	25	)	)	PUNCT
aiua-14101	152	26	.	.	PUNCT
aiua-14101	153	1	furthermore	furthermore	ADV
aiua-14101	153	2	,	,	PUNCT
aiua-14101	153	3	mrna	mrna	PROPN
aiua-14101	153	4	expression	expression	NOUN
aiua-14101	153	5	levels	level	NOUN
aiua-14101	153	6	in	in	ADP
aiua-14101	153	7	the	the	DET
aiua-14101	153	8	trehalose	trehalose	PROPN
aiua-14101	153	9	group	group	NOUN
aiua-14101	153	10	were	be	AUX
aiua-14101	153	11	significantly	significantly	ADV
aiua-14101	153	12	lower	low	ADJ
aiua-14101	153	13	than	than	ADP
aiua-14101	153	14	those	those	PRON
aiua-14101	153	15	observed	observe	VERB
aiua-14101	153	16	in	in	ADP
aiua-14101	153	17	the	the	DET
aiua-14101	153	18	tryptophan	tryptophan	NOUN
aiua-14101	153	19	group	group	NOUN
aiua-14101	153	20	(	(	PUNCT
aiua-14101	153	21	p	p	X
aiua-14101	153	22	<	<	X
aiua-14101	153	23	0.001	0.001	NUM
aiua-14101	153	24	)	)	PUNCT
aiua-14101	153	25	.	.	PUNCT
aiua-14101	154	1	bcl-2	bcl-2	VERB
aiua-14101	154	2	gene	gene	NOUN
aiua-14101	154	3	expression	expression	NOUN
aiua-14101	154	4	reached	reach	VERB
aiua-14101	154	5	its	its	PRON
aiua-14101	154	6	highest	high	ADJ
aiua-14101	154	7	level	level	NOUN
aiua-14101	154	8	in	in	ADP
aiua-14101	154	9	the	the	DET
aiua-14101	154	10	trehalose	trehalose	ADJ
aiua-14101	154	11	group	group	NOUN
aiua-14101	154	12	when	when	SCONJ
aiua-14101	154	13	compared	compare	VERB
aiua-14101	154	14	to	to	ADP
aiua-14101	154	15	the	the	DET
aiua-14101	154	16	control	control	NOUN
aiua-14101	154	17	and	and	CCONJ
aiua-14101	154	18	tryptophan	tryptophan	NOUN
aiua-14101	154	19	groups	group	NOUN
aiua-14101	154	20	(	(	PUNCT
aiua-14101	154	21	p	p	X
aiua-14101	154	22	<	<	X
aiua-14101	154	23	0.001	0.001	NUM
aiua-14101	154	24	)	)	PUNCT
aiua-14101	154	25	.	.	PUNCT
aiua-14101	155	1	no	no	DET
aiua-14101	155	2	statistically	statistically	ADV
aiua-14101	155	3	significant	significant	ADJ
aiua-14101	155	4	difference	difference	NOUN
aiua-14101	155	5	was	be	AUX
aiua-14101	155	6	detected	detect	VERB
aiua-14101	155	7	between	between	ADP
aiua-14101	155	8	the	the	DET
aiua-14101	155	9	control	control	NOUN
aiua-14101	155	10	and	and	CCONJ
aiua-14101	155	11	tryptophan	tryptophan	NOUN
aiua-14101	155	12	groups	group	NOUN
aiua-14101	155	13	in	in	ADP
aiua-14101	155	14	terms	term	NOUN
aiua-14101	155	15	of	of	ADP
aiua-14101	155	16	bcl-2	bcl-2	NOUN
aiua-14101	155	17	expression	expression	NOUN
aiua-14101	155	18	(	(	PUNCT
aiua-14101	155	19	p	p	X
aiua-14101	155	20	>	>	X
aiua-14101	155	21	0.05	0.05	NUM
aiua-14101	155	22	)	)	PUNCT
aiua-14101	155	23	.	.	PUNCT
aiua-14101	156	1	these	these	DET
aiua-14101	156	2	findings	finding	NOUN
aiua-14101	156	3	suggest	suggest	VERB
aiua-14101	156	4	that	that	SCONJ
aiua-14101	156	5	supplementation	supplementation	NOUN
aiua-14101	156	6	of	of	ADP
aiua-14101	156	7	the	the	DET
aiua-14101	156	8	cryopreservation	cryopreservation	NOUN
aiua-14101	156	9	extender	extender	NOUN
aiua-14101	156	10	with	with	ADP
aiua-14101	156	11	trehalose	trehalose	ADJ
aiua-14101	156	12	enhances	enhance	NOUN
aiua-14101	156	13	the	the	DET
aiua-14101	156	14	post	post	ADJ
aiua-14101	156	15	-	-	ADJ
aiua-14101	156	16	thaw	thaw	ADJ
aiua-14101	156	17	expression	expression	NOUN
aiua-14101	156	18	of	of	ADP
aiua-14101	156	19	the	the	DET
aiua-14101	156	20	antiapoptotic	antiapoptotic	ADJ
aiua-14101	156	21	bcl-2	bcl-2	ADJ
aiua-14101	156	22	gene	gene	NOUN
aiua-14101	156	23	.	.	PUNCT
aiua-14101	157	1	caspase-3	caspase-3	NUM
aiua-14101	157	2	gene	gene	NOUN
aiua-14101	157	3	expression	expression	NOUN
aiua-14101	157	4	did	do	AUX
aiua-14101	157	5	not	not	PART
aiua-14101	157	6	differ	differ	VERB
aiua-14101	157	7	significantly	significantly	ADV
aiua-14101	157	8	between	between	ADP
aiua-14101	157	9	the	the	DET
aiua-14101	157	10	tryptophan	tryptophan	NOUN
aiua-14101	157	11	and	and	CCONJ
aiua-14101	157	12	trehalose	trehalose	ADJ
aiua-14101	157	13	groups	group	NOUN
aiua-14101	157	14	(	(	PUNCT
aiua-14101	157	15	p	p	X
aiua-14101	157	16	>	>	X
aiua-14101	157	17	0.05	0.05	NUM
aiua-14101	157	18	)	)	PUNCT
aiua-14101	157	19	;	;	PUNCT
aiua-14101	157	20	however	however	ADV
aiua-14101	157	21	,	,	PUNCT
aiua-14101	157	22	when	when	SCONJ
aiua-14101	157	23	compared	compare	VERB
aiua-14101	157	24	to	to	ADP
aiua-14101	157	25	the	the	DET
aiua-14101	157	26	control	control	NOUN
aiua-14101	157	27	group	group	NOUN
aiua-14101	157	28	,	,	PUNCT
aiua-14101	157	29	both	both	CCONJ
aiua-14101	157	30	the	the	DET
aiua-14101	157	31	tryptophan	tryptophan	NOUN
aiua-14101	157	32	and	and	CCONJ
aiua-14101	157	33	trehalose	trehalose	PRON
aiua-14101	157	34	groups	group	NOUN
aiua-14101	157	35	showed	show	VERB
aiua-14101	157	36	a	a	DET
aiua-14101	157	37	significant	significant	ADJ
aiua-14101	157	38	decrease	decrease	NOUN
aiua-14101	157	39	in	in	ADP
aiua-14101	157	40	caspase-3	caspase-3	NUM
aiua-14101	157	41	expression	expression	NOUN
aiua-14101	157	42	levels	level	NOUN
aiua-14101	157	43	(	(	PUNCT
aiua-14101	157	44	p	p	X
aiua-14101	157	45	<	<	X
aiua-14101	157	46	0.05	0.05	NUM
aiua-14101	157	47	)	)	PUNCT
aiua-14101	157	48	.	.	PUNCT
aiua-14101	158	1	collectively	collectively	ADV
aiua-14101	158	2	,	,	PUNCT
aiua-14101	158	3	these	these	DET
aiua-14101	158	4	results	result	NOUN
aiua-14101	158	5	indicate	indicate	VERB
aiua-14101	158	6	that	that	SCONJ
aiua-14101	158	7	the	the	DET
aiua-14101	158	8	figure	figure	NOUN
aiua-14101	158	9	1	1	NUM
aiua-14101	158	10	.	.	PUNCT
aiua-14101	158	11	apoptotic	apoptotic	ADJ
aiua-14101	158	12	spermatozoa	spermatozoa	NOUN
aiua-14101	158	13	evaluation	evaluation	NOUN
aiua-14101	158	14	.	.	PUNCT
aiua-14101	159	1	apoptosis	apoptosis	NOUN
aiua-14101	159	2	was	be	AUX
aiua-14101	159	3	assessed	assess	VERB
aiua-14101	159	4	by	by	ADP
aiua-14101	159	5	annexin	annexin	NOUN
aiua-14101	159	6	v	v	NOUN
aiua-14101	159	7	-	-	PUNCT
aiua-14101	159	8	fitc	fitc	NOUN
aiua-14101	159	9	/	/	SYM
aiua-14101	159	10	pi	pi	NOUN
aiua-14101	159	11	assay	assay	ADV
aiua-14101	159	12	in	in	ADP
aiua-14101	159	13	epididymal	epididymal	ADJ
aiua-14101	159	14	rat	rat	NOUN
aiua-14101	159	15	sperm	sperm	NOUN
aiua-14101	159	16	.	.	PUNCT
aiua-14101	160	1	spermatozoa	spermatozoon	NOUN
aiua-14101	160	2	with	with	ADP
aiua-14101	160	3	completely	completely	ADV
aiua-14101	160	4	green	green	ADJ
aiua-14101	160	5	heads	head	NOUN
aiua-14101	160	6	were	be	AUX
aiua-14101	160	7	early	early	ADV
aiua-14101	160	8	apoptotic	apoptotic	ADJ
aiua-14101	160	9	(	(	PUNCT
aiua-14101	160	10	a	a	NOUN
aiua-14101	160	11	)	)	PUNCT
aiua-14101	160	12	,	,	PUNCT
aiua-14101	160	13	spermatozoa	spermatozoa	VERB
aiua-14101	160	14	with	with	ADP
aiua-14101	160	15	green	green	ADJ
aiua-14101	160	16	-	-	PUNCT
aiua-14101	160	17	red	red	ADJ
aiua-14101	160	18	heads	head	NOUN
aiua-14101	160	19	were	be	AUX
aiua-14101	160	20	late	late	ADV
aiua-14101	160	21	apoptotic	apoptotic	ADJ
aiua-14101	160	22	(	(	PUNCT
aiua-14101	160	23	b	b	X
aiua-14101	160	24	-	-	PUNCT
aiua-14101	160	25	c	c	NOUN
aiua-14101	160	26	)	)	PUNCT
aiua-14101	160	27	,	,	PUNCT
aiua-14101	160	28	spermatozoa	spermatozoa	VERB
aiua-14101	160	29	with	with	ADP
aiua-14101	160	30	completely	completely	ADV
aiua-14101	160	31	red	red	ADJ
aiua-14101	160	32	heads	head	NOUN
aiua-14101	160	33	were	be	AUX
aiua-14101	160	34	necrotic	necrotic	ADJ
aiua-14101	160	35	(	(	PUNCT
aiua-14101	160	36	d	d	NOUN
aiua-14101	160	37	)	)	PUNCT
aiua-14101	160	38	,	,	PUNCT
aiua-14101	160	39	and	and	CCONJ
aiua-14101	160	40	quantification	quantification	NOUN
aiua-14101	160	41	of	of	ADP
aiua-14101	160	42	the	the	DET
aiua-14101	160	43	apoptosis	apoptosis	NOUN
aiua-14101	160	44	index	index	NOUN
aiua-14101	160	45	(	(	PUNCT
aiua-14101	160	46	%	%	NOUN
aiua-14101	160	47	)	)	PUNCT
aiua-14101	160	48	of	of	ADP
aiua-14101	160	49	sperm	sperm	NOUN
aiua-14101	160	50	cells	cell	NOUN
aiua-14101	160	51	in	in	ADP
aiua-14101	160	52	each	each	DET
aiua-14101	160	53	group	group	NOUN
aiua-14101	160	54	(	(	PUNCT
aiua-14101	160	55	e	e	NOUN
aiua-14101	160	56	)	)	PUNCT
aiua-14101	160	57	.	.	PUNCT
aiua-14101	161	1	data	datum	NOUN
aiua-14101	161	2	were	be	AUX
aiua-14101	161	3	shown	show	VERB
aiua-14101	161	4	as	as	ADP
aiua-14101	161	5	the	the	DET
aiua-14101	161	6	mean	mean	NOUN
aiua-14101	161	7	±	±	PROPN
aiua-14101	161	8	se	se	X
aiua-14101	161	9	(	(	PUNCT
aiua-14101	161	10	n	n	NOUN
aiua-14101	161	11	=	=	SYM
aiua-14101	161	12	8	8	NUM
aiua-14101	161	13	in	in	ADP
aiua-14101	161	14	each	each	DET
aiua-14101	161	15	group	group	NOUN
aiua-14101	161	16	)	)	PUNCT
aiua-14101	161	17	.	.	PUNCT
aiua-14101	162	1	values	value	NOUN
aiua-14101	162	2	with	with	ADP
aiua-14101	162	3	different	different	ADJ
aiua-14101	162	4	superscripts	superscript	NOUN
aiua-14101	162	5	differ	differ	VERB
aiua-14101	162	6	significantly	significantly	ADV
aiua-14101	162	7	(	(	PUNCT
aiua-14101	162	8	p	p	X
aiua-14101	162	9	<	<	X
aiua-14101	162	10	0.001	0.001	NUM
aiua-14101	162	11	)	)	PUNCT
aiua-14101	162	12	.	.	PUNCT
aiua-14101	163	1	figure	figure	NOUN
aiua-14101	163	2	2	2	NUM
aiua-14101	163	3	.	.	PUNCT
aiua-14101	163	4	quantitative	quantitative	ADJ
aiua-14101	163	5	pcr	pcr	ADJ
aiua-14101	163	6	analysis	analysis	NOUN
aiua-14101	163	7	of	of	ADP
aiua-14101	163	8	the	the	DET
aiua-14101	163	9	relative	relative	ADJ
aiua-14101	163	10	expression	expression	NOUN
aiua-14101	163	11	of	of	ADP
aiua-14101	163	12	bax	bax	PROPN
aiua-14101	163	13	(	(	PUNCT
aiua-14101	163	14	a	a	NOUN
aiua-14101	163	15	)	)	PUNCT
aiua-14101	163	16	,	,	PUNCT
aiua-14101	163	17	bcl-2	bcl-2	PUNCT
aiua-14101	163	18	(	(	PUNCT
aiua-14101	163	19	b	b	NOUN
aiua-14101	163	20	)	)	PUNCT
aiua-14101	163	21	,	,	PUNCT
aiua-14101	163	22	and	and	CCONJ
aiua-14101	163	23	caspase-3	caspase-3	NUM
aiua-14101	163	24	genes	gene	NOUN
aiua-14101	163	25	(	(	PUNCT
aiua-14101	163	26	c	c	NOUN
aiua-14101	163	27	)	)	PUNCT
aiua-14101	163	28	on	on	ADP
aiua-14101	163	29	frozen	freeze	VERB
aiua-14101	163	30	-	-	PUNCT
aiua-14101	163	31	thawed	thaw	VERB
aiua-14101	163	32	epididymal	epididymal	ADJ
aiua-14101	163	33	rat	rat	NOUN
aiua-14101	163	34	sperm	sperm	NOUN
aiua-14101	163	35	.	.	PUNCT
aiua-14101	164	1	all	all	DET
aiua-14101	164	2	data	datum	NOUN
aiua-14101	164	3	were	be	AUX
aiua-14101	164	4	presented	present	VERB
aiua-14101	164	5	as	as	ADP
aiua-14101	164	6	mean	mean	PROPN
aiua-14101	164	7	±	±	NUM
aiua-14101	164	8	sem	sem	NOUN
aiua-14101	164	9	(	(	PUNCT
aiua-14101	164	10	n	n	NOUN
aiua-14101	164	11	=	=	SYM
aiua-14101	164	12	8	8	NUM
aiua-14101	164	13	in	in	ADP
aiua-14101	164	14	each	each	DET
aiua-14101	164	15	group	group	NOUN
aiua-14101	164	16	)	)	PUNCT
aiua-14101	164	17	.	.	PUNCT
aiua-14101	165	1	*	*	PUNCT
aiua-14101	165	2	p	p	X
aiua-14101	165	3	<	<	X
aiua-14101	165	4	0.05	0.05	NUM
aiua-14101	165	5	,	,	PUNCT
aiua-14101	165	6	*	*	PUNCT
aiua-14101	165	7	*	*	PUNCT
aiua-14101	165	8	p	p	X
aiua-14101	165	9	<	<	X
aiua-14101	165	10	0.01	0.01	NUM
aiua-14101	165	11	,	,	PUNCT
aiua-14101	165	12	and	and	CCONJ
aiua-14101	165	13	*	*	PUNCT
aiua-14101	165	14	*	*	PUNCT
aiua-14101	165	15	*	*	PUNCT
aiua-14101	165	16	p	p	X
aiua-14101	165	17	<	<	X
aiua-14101	165	18	0.001	0.001	NUM
aiua-14101	165	19	vs	vs	ADP
aiua-14101	165	20	the	the	DET
aiua-14101	165	21	other	other	ADJ
aiua-14101	165	22	group	group	NOUN
aiua-14101	165	23	.	.	PUNCT
aiua-14101	166	1	archivio	archivio	NOUN
aiua-14101	166	2	italiano	italiano	PROPN
aiua-14101	166	3	di	di	PROPN
aiua-14101	166	4	urologia	urologia	PROPN
aiua-14101	166	5	e	e	PROPN
aiua-14101	166	6	andrologia	andrologia	NOUN
aiua-14101	166	7	2025	2025	NUM
aiua-14101	166	8	;	;	PUNCT
aiua-14101	166	9	97(3):14101	97(3):14101	NUM
aiua-14101	166	10	f.	f.	PROPN
aiua-14101	166	11	yilmaz	yilmaz	PROPN
aiua-14101	166	12	dayanc	dayanc	PROPN
aiua-14101	166	13	,	,	PUNCT
aiua-14101	166	14	o.	o.	PROPN
aiua-14101	166	15	kaan	kaan	PROPN
aiua-14101	166	16	yalcin	yalcin	PROPN
aiua-14101	166	17	,	,	PUNCT
aiua-14101	166	18	a.	a.	NOUN
aiua-14101	166	19	eraslan	eraslan	PROPN
aiua-14101	166	20	sakar	sakar	PROPN
aiua-14101	166	21	6	6	NUM
aiua-14101	166	22	freeze	freeze	NOUN
aiua-14101	166	23	-	-	PUNCT
aiua-14101	166	24	thaw	thaw	NOUN
aiua-14101	166	25	process	process	NOUN
aiua-14101	166	26	induces	induce	VERB
aiua-14101	166	27	apoptosis	apoptosis	NOUN
aiua-14101	166	28	in	in	ADP
aiua-14101	166	29	rat	rat	NOUN
aiua-14101	166	30	spermatozoa	spermatozoon	NOUN
aiua-14101	166	31	,	,	PUNCT
aiua-14101	166	32	and	and	CCONJ
aiua-14101	166	33	the	the	DET
aiua-14101	166	34	addition	addition	NOUN
aiua-14101	166	35	of	of	ADP
aiua-14101	166	36	amino	amino	NOUN
aiua-14101	166	37	acid	acid	NOUN
aiua-14101	166	38	(	(	PUNCT
aiua-14101	166	39	tryptophan	tryptophan	NOUN
aiua-14101	166	40	)	)	PUNCT
aiua-14101	166	41	and	and	CCONJ
aiua-14101	166	42	disaccharide	disaccharide	NOUN
aiua-14101	166	43	(	(	PUNCT
aiua-14101	166	44	trehalose	trehalose	ADJ
aiua-14101	166	45	)	)	PUNCT
aiua-14101	166	46	to	to	ADP
aiua-14101	166	47	the	the	DET
aiua-14101	166	48	cryoprotectant	cryoprotectant	NOUN
aiua-14101	166	49	medium	medium	NOUN
aiua-14101	166	50	reduces	reduce	VERB
aiua-14101	166	51	the	the	DET
aiua-14101	166	52	expression	expression	NOUN
aiua-14101	166	53	levels	level	NOUN
aiua-14101	166	54	of	of	ADP
aiua-14101	166	55	genes	gene	NOUN
aiua-14101	166	56	involved	involve	VERB
aiua-14101	166	57	in	in	ADP
aiua-14101	166	58	the	the	DET
aiua-14101	166	59	apoptotic	apoptotic	ADJ
aiua-14101	166	60	pathway	pathway	NOUN
aiua-14101	166	61	after	after	ADP
aiua-14101	166	62	thawing	thaw	VERB
aiua-14101	166	63	.	.	PUNCT
aiua-14101	167	1	discussion	discussion	NOUN
aiua-14101	167	2	cryopreservation	cryopreservation	NOUN
aiua-14101	167	3	is	be	AUX
aiua-14101	167	4	known	know	VERB
aiua-14101	167	5	to	to	PART
aiua-14101	167	6	be	be	AUX
aiua-14101	167	7	a	a	DET
aiua-14101	167	8	more	more	ADV
aiua-14101	167	9	challenging	challenging	ADJ
aiua-14101	167	10	process	process	NOUN
aiua-14101	167	11	for	for	ADP
aiua-14101	167	12	rat	rat	NOUN
aiua-14101	167	13	sperm	sperm	NOUN
aiua-14101	167	14	compared	compare	VERB
aiua-14101	167	15	to	to	ADP
aiua-14101	167	16	other	other	ADJ
aiua-14101	167	17	mammalian	mammalian	ADJ
aiua-14101	167	18	species	specie	NOUN
aiua-14101	167	19	(	(	PUNCT
aiua-14101	167	20	6	6	NUM
aiua-14101	167	21	,	,	PUNCT
aiua-14101	167	22	22	22	NUM
aiua-14101	167	23	)	)	PUNCT
aiua-14101	167	24	.	.	PUNCT
aiua-14101	168	1	main	main	ADJ
aiua-14101	168	2	reason	reason	NOUN
aiua-14101	168	3	for	for	ADP
aiua-14101	168	4	this	this	PRON
aiua-14101	168	5	is	be	AUX
aiua-14101	168	6	that	that	SCONJ
aiua-14101	168	7	rat	rat	NOUN
aiua-14101	168	8	spermatozoa	spermatozoon	NOUN
aiua-14101	168	9	are	be	AUX
aiua-14101	168	10	highly	highly	ADV
aiua-14101	168	11	sensitive	sensitive	ADJ
aiua-14101	168	12	to	to	ADP
aiua-14101	168	13	all	all	DET
aiua-14101	168	14	kinds	kind	NOUN
aiua-14101	168	15	of	of	ADP
aiua-14101	168	16	manipulations	manipulation	NOUN
aiua-14101	168	17	and	and	CCONJ
aiua-14101	168	18	that	that	SCONJ
aiua-14101	168	19	these	these	DET
aiua-14101	168	20	procedures	procedure	NOUN
aiua-14101	168	21	constitute	constitute	VERB
aiua-14101	168	22	a	a	DET
aiua-14101	168	23	significant	significant	ADJ
aiua-14101	168	24	source	source	NOUN
aiua-14101	168	25	of	of	ADP
aiua-14101	168	26	stress	stress	NOUN
aiua-14101	168	27	on	on	ADP
aiua-14101	168	28	the	the	DET
aiua-14101	168	29	cell	cell	NOUN
aiua-14101	168	30	.	.	PUNCT
aiua-14101	169	1	furthermore	furthermore	ADV
aiua-14101	169	2	,	,	PUNCT
aiua-14101	169	3	the	the	DET
aiua-14101	169	4	molecular	molecular	ADJ
aiua-14101	169	5	structure	structure	NOUN
aiua-14101	169	6	of	of	ADP
aiua-14101	169	7	rat	rat	NOUN
aiua-14101	169	8	sperm	sperm	NOUN
aiua-14101	169	9	is	be	AUX
aiua-14101	169	10	remarkably	remarkably	ADV
aiua-14101	169	11	different	different	ADJ
aiua-14101	169	12	from	from	ADP
aiua-14101	169	13	that	that	PRON
aiua-14101	169	14	of	of	ADP
aiua-14101	169	15	other	other	ADJ
aiua-14101	169	16	mammalian	mammalian	ADJ
aiua-14101	169	17	sperm	sperm	NOUN
aiua-14101	169	18	,	,	PUNCT
aiua-14101	169	19	especially	especially	ADV
aiua-14101	169	20	due	due	ADP
aiua-14101	169	21	to	to	ADP
aiua-14101	169	22	differences	difference	NOUN
aiua-14101	169	23	in	in	ADP
aiua-14101	169	24	the	the	DET
aiua-14101	169	25	lipid	lipid	NOUN
aiua-14101	169	26	content	content	NOUN
aiua-14101	169	27	and	and	CCONJ
aiua-14101	169	28	composition	composition	NOUN
aiua-14101	169	29	of	of	ADP
aiua-14101	169	30	the	the	DET
aiua-14101	169	31	sperm	sperm	NOUN
aiua-14101	169	32	membrane	membrane	NOUN
aiua-14101	169	33	(	(	PUNCT
aiua-14101	169	34	23	23	NUM
aiua-14101	169	35	,	,	PUNCT
aiua-14101	169	36	24	24	NUM
aiua-14101	169	37	)	)	PUNCT
aiua-14101	169	38	.	.	PUNCT
aiua-14101	170	1	motility	motility	NOUN
aiua-14101	170	2	is	be	AUX
aiua-14101	170	3	considered	consider	VERB
aiua-14101	170	4	a	a	DET
aiua-14101	170	5	criterion	criterion	NOUN
aiua-14101	170	6	of	of	ADP
aiua-14101	170	7	cell	cell	NOUN
aiua-14101	170	8	activity	activity	NOUN
aiua-14101	170	9	and	and	CCONJ
aiua-14101	170	10	viability	viability	NOUN
aiua-14101	170	11	after	after	ADP
aiua-14101	170	12	cryopreservation	cryopreservation	NOUN
aiua-14101	170	13	.	.	PUNCT
aiua-14101	171	1	in	in	ADP
aiua-14101	171	2	this	this	DET
aiua-14101	171	3	study	study	NOUN
aiua-14101	171	4	,	,	PUNCT
aiua-14101	171	5	motility	motility	NOUN
aiua-14101	171	6	was	be	AUX
aiua-14101	171	7	found	find	VERB
aiua-14101	171	8	to	to	PART
aiua-14101	171	9	be	be	AUX
aiua-14101	171	10	higher	high	ADJ
aiua-14101	171	11	in	in	ADP
aiua-14101	171	12	the	the	DET
aiua-14101	171	13	control	control	NOUN
aiua-14101	171	14	group	group	NOUN
aiua-14101	171	15	(	(	PUNCT
aiua-14101	171	16	9.75	9.75	NUM
aiua-14101	171	17	±	±	NUM
aiua-14101	171	18	1.33	1.33	NUM
aiua-14101	171	19	)	)	PUNCT
aiua-14101	171	20	compared	compare	VERB
aiua-14101	171	21	to	to	ADP
aiua-14101	171	22	the	the	DET
aiua-14101	171	23	trehalose	trehalose	PROPN
aiua-14101	171	24	group	group	NOUN
aiua-14101	171	25	(	(	PUNCT
aiua-14101	171	26	6.87	6.87	NUM
aiua-14101	171	27	±	±	NUM
aiua-14101	171	28	2.48	2.48	NUM
aiua-14101	171	29	)	)	PUNCT
aiua-14101	171	30	,	,	PUNCT
aiua-14101	171	31	but	but	CCONJ
aiua-14101	171	32	the	the	DET
aiua-14101	171	33	difference	difference	NOUN
aiua-14101	171	34	was	be	AUX
aiua-14101	171	35	not	not	PART
aiua-14101	171	36	statistically	statistically	ADV
aiua-14101	171	37	significant	significant	ADJ
aiua-14101	171	38	(	(	PUNCT
aiua-14101	171	39	p	p	X
aiua-14101	171	40	>	>	X
aiua-14101	171	41	0.05	0.05	NUM
aiua-14101	171	42	)	)	PUNCT
aiua-14101	171	43	.	.	PUNCT
aiua-14101	172	1	interestingly	interestingly	ADV
aiua-14101	172	2	,	,	PUNCT
aiua-14101	172	3	although	although	SCONJ
aiua-14101	172	4	the	the	DET
aiua-14101	172	5	freezing	freezing	ADJ
aiua-14101	172	6	protocol	protocol	NOUN
aiua-14101	172	7	used	use	VERB
aiua-14101	172	8	in	in	ADP
aiua-14101	172	9	this	this	DET
aiua-14101	172	10	study	study	NOUN
aiua-14101	172	11	was	be	AUX
aiua-14101	172	12	the	the	DET
aiua-14101	172	13	same	same	ADJ
aiua-14101	172	14	as	as	ADP
aiua-14101	172	15	the	the	DET
aiua-14101	172	16	protocol	protocol	NOUN
aiua-14101	172	17	reported	report	VERB
aiua-14101	172	18	by	by	ADP
aiua-14101	172	19	nakatsukasa	nakatsukasa	PROPN
aiua-14101	172	20	et	et	PROPN
aiua-14101	172	21	al	al	PROPN
aiua-14101	172	22	.	.	PROPN
aiua-14101	173	1	(	(	PUNCT
aiua-14101	173	2	6	6	NUM
aiua-14101	173	3	)	)	PUNCT
aiua-14101	173	4	,	,	PUNCT
aiua-14101	173	5	the	the	DET
aiua-14101	173	6	addition	addition	NOUN
aiua-14101	173	7	of	of	ADP
aiua-14101	173	8	100	100	NUM
aiua-14101	173	9	mm	mm	NOUN
aiua-14101	173	10	trehalose	trehalose	INTJ
aiua-14101	173	11	to	to	ADP
aiua-14101	173	12	the	the	DET
aiua-14101	173	13	diluent	diluent	NOUN
aiua-14101	173	14	increased	increase	VERB
aiua-14101	173	15	the	the	DET
aiua-14101	173	16	post	post	ADJ
aiua-14101	173	17	-	-	ADJ
aiua-14101	173	18	thaw	thaw	ADJ
aiua-14101	173	19	motility	motility	NOUN
aiua-14101	173	20	(	(	PUNCT
aiua-14101	173	21	6.87	6.87	NUM
aiua-14101	173	22	±	±	NUM
aiua-14101	173	23	2.48	2.48	NUM
aiua-14101	173	24	)	)	PUNCT
aiua-14101	173	25	,	,	PUNCT
aiua-14101	173	26	which	which	PRON
aiua-14101	173	27	was	be	AUX
aiua-14101	173	28	higher	high	ADJ
aiua-14101	173	29	than	than	ADP
aiua-14101	173	30	the	the	DET
aiua-14101	173	31	value	value	NOUN
aiua-14101	173	32	reported	report	VERB
aiua-14101	173	33	by	by	ADP
aiua-14101	173	34	nakatsukasa	nakatsukasa	PROPN
aiua-14101	173	35	et	et	PROPN
aiua-14101	173	36	al	al	PROPN
aiua-14101	173	37	.	.	PROPN
aiua-14101	174	1	(	(	PUNCT
aiua-14101	174	2	6	6	NUM
aiua-14101	174	3	)	)	PUNCT
aiua-14101	174	4	(	(	PUNCT
aiua-14101	174	5	4.6	4.6	NUM
aiua-14101	174	6	±	±	NUM
aiua-14101	174	7	0.1	0.1	NUM
aiua-14101	174	8	)	)	PUNCT
aiua-14101	174	9	.	.	PUNCT
aiua-14101	175	1	moreover	moreover	ADV
aiua-14101	175	2	,	,	PUNCT
aiua-14101	175	3	in	in	ADP
aiua-14101	175	4	another	another	DET
aiua-14101	175	5	study	study	NOUN
aiua-14101	175	6	conducted	conduct	VERB
aiua-14101	175	7	by	by	ADP
aiua-14101	175	8	the	the	DET
aiua-14101	175	9	same	same	ADJ
aiua-14101	175	10	author	author	NOUN
aiua-14101	175	11	(	(	PUNCT
aiua-14101	175	12	2	2	NUM
aiua-14101	175	13	)	)	PUNCT
aiua-14101	175	14	,	,	PUNCT
aiua-14101	175	15	post	post	ADJ
aiua-14101	175	16	-	-	ADJ
aiua-14101	175	17	thaw	thaw	ADJ
aiua-14101	175	18	motility	motility	NOUN
aiua-14101	175	19	ratios	ratio	NOUN
aiua-14101	175	20	of	of	ADP
aiua-14101	175	21	rat	rat	NOUN
aiua-14101	175	22	sperm	sperm	NOUN
aiua-14101	175	23	was	be	AUX
aiua-14101	175	24	reported	report	VERB
aiua-14101	175	25	to	to	PART
aiua-14101	175	26	range	range	VERB
aiua-14101	175	27	from	from	ADP
aiua-14101	175	28	2	2	NUM
aiua-14101	175	29	%	%	NOUN
aiua-14101	175	30	to	to	ADP
aiua-14101	175	31	12.3	12.3	NUM
aiua-14101	175	32	%	%	NOUN
aiua-14101	175	33	.	.	PUNCT
aiua-14101	176	1	the	the	DET
aiua-14101	176	2	motility	motility	NOUN
aiua-14101	176	3	observed	observe	VERB
aiua-14101	176	4	in	in	ADP
aiua-14101	176	5	the	the	DET
aiua-14101	176	6	present	present	ADJ
aiua-14101	176	7	study	study	NOUN
aiua-14101	176	8	with	with	ADP
aiua-14101	176	9	100	100	NUM
aiua-14101	176	10	mm	mm	NOUN
aiua-14101	176	11	trehalose	trehalose	PROPN
aiua-14101	176	12	falls	fall	VERB
aiua-14101	176	13	within	within	ADP
aiua-14101	176	14	this	this	DET
aiua-14101	176	15	range	range	NOUN
aiua-14101	176	16	,	,	PUNCT
aiua-14101	176	17	suggesting	suggest	VERB
aiua-14101	176	18	that	that	SCONJ
aiua-14101	176	19	trehalose	trehalose	PRON
aiua-14101	176	20	contributes	contribute	VERB
aiua-14101	176	21	to	to	ADP
aiua-14101	176	22	maintaining	maintain	VERB
aiua-14101	176	23	motility	motility	NOUN
aiua-14101	176	24	after	after	ADP
aiua-14101	176	25	freezing	freeze	VERB
aiua-14101	176	26	and	and	CCONJ
aiua-14101	176	27	thawing	thaw	VERB
aiua-14101	176	28	.	.	PUNCT
aiua-14101	177	1	similar	similar	ADJ
aiua-14101	177	2	stabilizing	stabilizing	NOUN
aiua-14101	177	3	effects	effect	NOUN
aiua-14101	177	4	of	of	ADP
aiua-14101	177	5	trehalose	trehalose	PRON
aiua-14101	177	6	have	have	AUX
aiua-14101	177	7	also	also	ADV
aiua-14101	177	8	been	be	AUX
aiua-14101	177	9	reported	report	VERB
aiua-14101	177	10	in	in	ADP
aiua-14101	177	11	other	other	ADJ
aiua-14101	177	12	species	specie	NOUN
aiua-14101	177	13	,	,	PUNCT
aiua-14101	177	14	including	include	VERB
aiua-14101	177	15	ram	ram	NOUN
aiua-14101	177	16	(	(	PUNCT
aiua-14101	177	17	25	25	NUM
aiua-14101	177	18	)	)	PUNCT
aiua-14101	177	19	and	and	CCONJ
aiua-14101	177	20	goat	goat	PROPN
aiua-14101	177	21	(	(	PUNCT
aiua-14101	177	22	26	26	NUM
aiua-14101	177	23	)	)	PUNCT
aiua-14101	177	24	sperm	sperm	NOUN
aiua-14101	177	25	.	.	PUNCT
aiua-14101	178	1	in	in	ADP
aiua-14101	178	2	contrast	contrast	NOUN
aiua-14101	178	3	,	,	PUNCT
aiua-14101	178	4	tryptophan	tryptophan	NOUN
aiua-14101	178	5	at	at	ADP
aiua-14101	178	6	25	25	NUM
aiua-14101	178	7	mm	mm	NOUN
aiua-14101	178	8	had	have	VERB
aiua-14101	178	9	minimal	minimal	ADJ
aiua-14101	178	10	impact	impact	NOUN
aiua-14101	178	11	on	on	ADP
aiua-14101	178	12	motility	motility	NOUN
aiua-14101	178	13	.	.	PUNCT
aiua-14101	179	1	similar	similar	ADJ
aiua-14101	179	2	findings	finding	NOUN
aiua-14101	179	3	were	be	AUX
aiua-14101	179	4	reported	report	VERB
aiua-14101	179	5	by	by	ADP
aiua-14101	179	6	koçak	koçak	NOUN
aiua-14101	179	7	and	and	CCONJ
aiua-14101	179	8	yıldız	yıldız	PROPN
aiua-14101	179	9	(	(	PUNCT
aiua-14101	179	10	27	27	NUM
aiua-14101	179	11	)	)	PUNCT
aiua-14101	179	12	in	in	ADP
aiua-14101	179	13	mouse	mouse	NOUN
aiua-14101	179	14	sperm	sperm	NOUN
aiua-14101	179	15	,	,	PUNCT
aiua-14101	179	16	where	where	SCONJ
aiua-14101	179	17	the	the	DET
aiua-14101	179	18	same	same	ADJ
aiua-14101	179	19	concentration	concentration	NOUN
aiua-14101	179	20	had	have	VERB
aiua-14101	179	21	only	only	ADV
aiua-14101	179	22	a	a	DET
aiua-14101	179	23	modest	modest	ADJ
aiua-14101	179	24	effect	effect	NOUN
aiua-14101	179	25	on	on	ADP
aiua-14101	179	26	motility	motility	NOUN
aiua-14101	179	27	(	(	PUNCT
aiua-14101	179	28	20.83	20.83	NUM
aiua-14101	179	29	±	±	NUM
aiua-14101	179	30	3.96	3.96	NUM
aiua-14101	179	31	)	)	PUNCT
aiua-14101	179	32	but	but	CCONJ
aiua-14101	179	33	improved	improve	VERB
aiua-14101	179	34	fertilization	fertilization	NOUN
aiua-14101	179	35	capacity	capacity	NOUN
aiua-14101	179	36	.	.	PUNCT
aiua-14101	180	1	this	this	PRON
aiua-14101	180	2	suggests	suggest	VERB
aiua-14101	180	3	that	that	SCONJ
aiua-14101	180	4	tryptophan	tryptophan	NOUN
aiua-14101	180	5	’s	’s	PART
aiua-14101	180	6	effect	effect	NOUN
aiua-14101	180	7	on	on	ADP
aiua-14101	180	8	motility	motility	NOUN
aiua-14101	180	9	is	be	AUX
aiua-14101	180	10	indirect	indirect	ADJ
aiua-14101	180	11	and	and	CCONJ
aiua-14101	180	12	may	may	AUX
aiua-14101	180	13	depend	depend	VERB
aiua-14101	180	14	on	on	ADP
aiua-14101	180	15	its	its	PRON
aiua-14101	180	16	antioxidant	antioxidant	ADJ
aiua-14101	180	17	activity	activity	NOUN
aiua-14101	180	18	rather	rather	ADV
aiua-14101	180	19	than	than	ADP
aiua-14101	180	20	structural	structural	ADJ
aiua-14101	180	21	stabilization	stabilization	NOUN
aiua-14101	180	22	(	(	PUNCT
aiua-14101	180	23	28	28	NUM
aiua-14101	180	24	)	)	PUNCT
aiua-14101	180	25	.	.	PUNCT
aiua-14101	181	1	when	when	SCONJ
aiua-14101	181	2	viability	viability	NOUN
aiua-14101	181	3	rate	rate	NOUN
aiua-14101	181	4	was	be	AUX
aiua-14101	181	5	considered	consider	VERB
aiua-14101	181	6	,	,	PUNCT
aiua-14101	181	7	the	the	DET
aiua-14101	181	8	highest	high	ADJ
aiua-14101	181	9	postthaw	postthaw	NOUN
aiua-14101	181	10	viability	viability	NOUN
aiua-14101	181	11	(	(	PUNCT
aiua-14101	181	12	26.25	26.25	NUM
aiua-14101	181	13	±	±	NUM
aiua-14101	181	14	3.10	3.10	NUM
aiua-14101	181	15	)	)	PUNCT
aiua-14101	181	16	was	be	AUX
aiua-14101	181	17	observed	observe	VERB
aiua-14101	181	18	in	in	ADP
aiua-14101	181	19	the	the	DET
aiua-14101	181	20	trehalose	trehalose	ADJ
aiua-14101	181	21	group	group	NOUN
aiua-14101	181	22	and	and	CCONJ
aiua-14101	181	23	was	be	AUX
aiua-14101	181	24	significantly	significantly	ADV
aiua-14101	181	25	higher	high	ADJ
aiua-14101	181	26	than	than	ADP
aiua-14101	181	27	in	in	ADP
aiua-14101	181	28	the	the	DET
aiua-14101	181	29	tryptophan	tryptophan	NOUN
aiua-14101	181	30	group	group	NOUN
aiua-14101	181	31	(	(	PUNCT
aiua-14101	181	32	p	p	X
aiua-14101	181	33	<	<	X
aiua-14101	181	34	0.05	0.05	NUM
aiua-14101	181	35	)	)	PUNCT
aiua-14101	181	36	.	.	PUNCT
aiua-14101	182	1	trehalose	trehalose	INTJ
aiua-14101	182	2	,	,	PUNCT
aiua-14101	182	3	as	as	ADP
aiua-14101	182	4	a	a	DET
aiua-14101	182	5	non	non	ADJ
aiua-14101	182	6	-	-	ADJ
aiua-14101	182	7	permeable	permeable	ADJ
aiua-14101	182	8	disaccharide	disaccharide	NOUN
aiua-14101	182	9	,	,	PUNCT
aiua-14101	182	10	reduces	reduce	VERB
aiua-14101	182	11	osmotic	osmotic	ADJ
aiua-14101	182	12	stress	stress	NOUN
aiua-14101	182	13	during	during	ADP
aiua-14101	182	14	dehydration	dehydration	NOUN
aiua-14101	182	15	and	and	CCONJ
aiua-14101	182	16	stabilizes	stabilize	VERB
aiua-14101	182	17	sperm	sperm	NOUN
aiua-14101	182	18	cell	cell	NOUN
aiua-14101	182	19	structures	structure	NOUN
aiua-14101	182	20	,	,	PUNCT
aiua-14101	182	21	leading	lead	VERB
aiua-14101	182	22	to	to	ADP
aiua-14101	182	23	better	well	ADJ
aiua-14101	182	24	survival	survival	NOUN
aiua-14101	182	25	(	(	PUNCT
aiua-14101	182	26	29	29	NUM
aiua-14101	182	27	)	)	PUNCT
aiua-14101	182	28	.	.	PUNCT
aiua-14101	183	1	this	this	DET
aiua-14101	183	2	result	result	NOUN
aiua-14101	183	3	is	be	AUX
aiua-14101	183	4	consistent	consistent	ADJ
aiua-14101	183	5	with	with	ADP
aiua-14101	183	6	findings	finding	NOUN
aiua-14101	183	7	in	in	ADP
aiua-14101	183	8	pig	pig	NOUN
aiua-14101	183	9	(	(	PUNCT
aiua-14101	183	10	30	30	NUM
aiua-14101	183	11	)	)	PUNCT
aiua-14101	183	12	,	,	PUNCT
aiua-14101	183	13	ram	ram	NOUN
aiua-14101	183	14	(	(	PUNCT
aiua-14101	183	15	31	31	NUM
aiua-14101	183	16	)	)	PUNCT
aiua-14101	183	17	,	,	PUNCT
aiua-14101	183	18	human	human	NOUN
aiua-14101	183	19	(	(	PUNCT
aiua-14101	183	20	32	32	NUM
aiua-14101	183	21	)	)	PUNCT
aiua-14101	183	22	,	,	PUNCT
aiua-14101	183	23	fish	fish	NOUN
aiua-14101	183	24	(	(	PUNCT
aiua-14101	183	25	33	33	NUM
aiua-14101	183	26	,	,	PUNCT
aiua-14101	183	27	34	34	NUM
aiua-14101	183	28	)	)	PUNCT
aiua-14101	183	29	,	,	PUNCT
aiua-14101	183	30	rabbit	rabbit	NOUN
aiua-14101	183	31	(	(	PUNCT
aiua-14101	183	32	35	35	NUM
aiua-14101	183	33	)	)	PUNCT
aiua-14101	183	34	,	,	PUNCT
aiua-14101	183	35	buffalo	buffalo	NOUN
aiua-14101	183	36	(	(	PUNCT
aiua-14101	183	37	36	36	NUM
aiua-14101	183	38	)	)	PUNCT
aiua-14101	183	39	,	,	PUNCT
aiua-14101	183	40	and	and	CCONJ
aiua-14101	183	41	mouse	mouse	NOUN
aiua-14101	183	42	(	(	PUNCT
aiua-14101	183	43	27	27	NUM
aiua-14101	183	44	)	)	PUNCT
aiua-14101	183	45	sperm	sperm	NOUN
aiua-14101	183	46	cryopreservation	cryopreservation	NOUN
aiua-14101	183	47	,	,	PUNCT
aiua-14101	183	48	where	where	SCONJ
aiua-14101	183	49	trehalose	trehalose	PRON
aiua-14101	183	50	enhanced	enhanced	ADJ
aiua-14101	183	51	viability	viability	NOUN
aiua-14101	183	52	.	.	PUNCT
aiua-14101	184	1	by	by	ADP
aiua-14101	184	2	comparison	comparison	NOUN
aiua-14101	184	3	,	,	PUNCT
aiua-14101	184	4	tryptophan	tryptophan	NOUN
aiua-14101	184	5	showed	show	VERB
aiua-14101	184	6	weaker	weak	ADJ
aiua-14101	184	7	effects	effect	NOUN
aiua-14101	184	8	on	on	ADP
aiua-14101	184	9	viability	viability	NOUN
aiua-14101	184	10	,	,	PUNCT
aiua-14101	184	11	likely	likely	ADJ
aiua-14101	184	12	because	because	SCONJ
aiua-14101	184	13	it	it	PRON
aiua-14101	184	14	mainly	mainly	ADV
aiua-14101	184	15	reduces	reduce	VERB
aiua-14101	184	16	oxidative	oxidative	ADJ
aiua-14101	184	17	stress	stress	NOUN
aiua-14101	184	18	without	without	ADP
aiua-14101	184	19	improving	improve	VERB
aiua-14101	184	20	osmotic	osmotic	ADJ
aiua-14101	184	21	stability	stability	NOUN
aiua-14101	184	22	.	.	PUNCT
aiua-14101	185	1	since	since	SCONJ
aiua-14101	185	2	rat	rat	NOUN
aiua-14101	185	3	sperm	sperm	NOUN
aiua-14101	185	4	membranes	membrane	NOUN
aiua-14101	185	5	have	have	VERB
aiua-14101	185	6	a	a	DET
aiua-14101	185	7	high	high	ADJ
aiua-14101	185	8	lipid	lipid	NOUN
aiua-14101	185	9	content	content	NOUN
aiua-14101	185	10	and	and	CCONJ
aiua-14101	185	11	are	be	AUX
aiua-14101	185	12	prone	prone	ADJ
aiua-14101	185	13	to	to	AUX
aiua-14101	185	14	oxidative	oxidative	ADJ
aiua-14101	185	15	damage	damage	NOUN
aiua-14101	185	16	(	(	PUNCT
aiua-14101	185	17	28	28	NUM
aiua-14101	185	18	,	,	PUNCT
aiua-14101	185	19	37	37	NUM
aiua-14101	185	20	)	)	PUNCT
aiua-14101	185	21	,	,	PUNCT
aiua-14101	185	22	tryptophan	tryptophan	NOUN
aiua-14101	185	23	may	may	AUX
aiua-14101	185	24	have	have	VERB
aiua-14101	185	25	more	more	ADJ
aiua-14101	185	26	impact	impact	NOUN
aiua-14101	185	27	on	on	ADP
aiua-14101	185	28	dna	dna	NOUN
aiua-14101	185	29	or	or	CCONJ
aiua-14101	185	30	membrane	membrane	NOUN
aiua-14101	185	31	integrity	integrity	NOUN
aiua-14101	185	32	than	than	ADP
aiua-14101	185	33	on	on	ADP
aiua-14101	185	34	viability	viability	NOUN
aiua-14101	185	35	itself	itself	PRON
aiua-14101	185	36	.	.	PUNCT
aiua-14101	186	1	plasma	plasma	NOUN
aiua-14101	186	2	membrane	membrane	NOUN
aiua-14101	186	3	integrity	integrity	NOUN
aiua-14101	186	4	is	be	AUX
aiua-14101	186	5	a	a	DET
aiua-14101	186	6	critical	critical	ADJ
aiua-14101	186	7	indicator	indicator	NOUN
aiua-14101	186	8	for	for	ADP
aiua-14101	186	9	assessing	assess	VERB
aiua-14101	186	10	sperm	sperm	NOUN
aiua-14101	186	11	quality	quality	NOUN
aiua-14101	186	12	and	and	CCONJ
aiua-14101	186	13	reproductive	reproductive	ADJ
aiua-14101	186	14	potential	potential	NOUN
aiua-14101	186	15	after	after	ADP
aiua-14101	186	16	cryopreservation	cryopreservation	NOUN
aiua-14101	186	17	.	.	PUNCT
aiua-14101	187	1	the	the	DET
aiua-14101	187	2	trehalose	trehalose	PROPN
aiua-14101	187	3	group	group	NOUN
aiua-14101	187	4	maintained	maintain	VERB
aiua-14101	187	5	the	the	DET
aiua-14101	187	6	highest	high	ADJ
aiua-14101	187	7	membrane	membrane	NOUN
aiua-14101	187	8	integrity	integrity	NOUN
aiua-14101	187	9	(	(	PUNCT
aiua-14101	187	10	20.25	20.25	NUM
aiua-14101	187	11	±	±	NUM
aiua-14101	187	12	3.65	3.65	NUM
aiua-14101	187	13	)	)	PUNCT
aiua-14101	187	14	,	,	PUNCT
aiua-14101	187	15	while	while	SCONJ
aiua-14101	187	16	tryptophan	tryptophan	NOUN
aiua-14101	187	17	had	have	VERB
aiua-14101	187	18	a	a	DET
aiua-14101	187	19	reduced	reduced	ADJ
aiua-14101	187	20	effect	effect	NOUN
aiua-14101	187	21	(	(	PUNCT
aiua-14101	187	22	p	p	X
aiua-14101	187	23	<	<	X
aiua-14101	187	24	0.05	0.05	NUM
aiua-14101	187	25	)	)	PUNCT
aiua-14101	187	26	.	.	PUNCT
aiua-14101	188	1	trehalose	trehalose	PROPN
aiua-14101	188	2	likely	likely	ADV
aiua-14101	188	3	provides	provide	VERB
aiua-14101	188	4	superior	superior	ADJ
aiua-14101	188	5	protection	protection	NOUN
aiua-14101	188	6	by	by	ADP
aiua-14101	188	7	stabilizing	stabilize	VERB
aiua-14101	188	8	the	the	DET
aiua-14101	188	9	plasma	plasma	NOUN
aiua-14101	188	10	membrane	membrane	NOUN
aiua-14101	188	11	bilayer	bilayer	NOUN
aiua-14101	188	12	and	and	CCONJ
aiua-14101	188	13	minimizing	minimize	VERB
aiua-14101	188	14	osmotic	osmotic	ADJ
aiua-14101	188	15	imbalance	imbalance	NOUN
aiua-14101	188	16	during	during	ADP
aiua-14101	188	17	freezing	freezing	NOUN
aiua-14101	188	18	(	(	PUNCT
aiua-14101	188	19	35	35	NUM
aiua-14101	188	20	)	)	PUNCT
aiua-14101	188	21	.	.	PUNCT
aiua-14101	189	1	this	this	PRON
aiua-14101	189	2	is	be	AUX
aiua-14101	189	3	consistent	consistent	ADJ
aiua-14101	189	4	with	with	ADP
aiua-14101	189	5	studies	study	NOUN
aiua-14101	189	6	on	on	ADP
aiua-14101	189	7	ram	ram	NOUN
aiua-14101	189	8	(	(	PUNCT
aiua-14101	189	9	25	25	NUM
aiua-14101	189	10	,	,	PUNCT
aiua-14101	189	11	38	38	NUM
aiua-14101	189	12	)	)	PUNCT
aiua-14101	189	13	,	,	PUNCT
aiua-14101	189	14	goat	goat	PROPN
aiua-14101	189	15	(	(	PUNCT
aiua-14101	189	16	26	26	NUM
aiua-14101	189	17	)	)	PUNCT
aiua-14101	189	18	,	,	PUNCT
aiua-14101	189	19	and	and	CCONJ
aiua-14101	189	20	bull	bull	NOUN
aiua-14101	189	21	(	(	PUNCT
aiua-14101	189	22	39	39	NUM
aiua-14101	189	23	)	)	PUNCT
aiua-14101	189	24	sperm	sperm	NOUN
aiua-14101	189	25	where	where	SCONJ
aiua-14101	189	26	trehalose	trehalose	ADJ
aiua-14101	189	27	preserved	preserve	VERB
aiua-14101	189	28	membrane	membrane	NOUN
aiua-14101	189	29	structure	structure	NOUN
aiua-14101	189	30	more	more	ADV
aiua-14101	189	31	effectively	effectively	ADV
aiua-14101	189	32	than	than	ADP
aiua-14101	189	33	amino	amino	ADJ
aiua-14101	189	34	acids	acid	NOUN
aiua-14101	189	35	.	.	PUNCT
aiua-14101	190	1	sperm	sperm	NOUN
aiua-14101	190	2	concentration	concentration	NOUN
aiua-14101	190	3	did	do	AUX
aiua-14101	190	4	not	not	PART
aiua-14101	190	5	differ	differ	VERB
aiua-14101	190	6	significantly	significantly	ADV
aiua-14101	190	7	among	among	ADP
aiua-14101	190	8	the	the	DET
aiua-14101	190	9	experimental	experimental	ADJ
aiua-14101	190	10	groups	group	NOUN
aiua-14101	190	11	.	.	PUNCT
aiua-14101	191	1	this	this	PRON
aiua-14101	191	2	was	be	AUX
aiua-14101	191	3	expected	expect	VERB
aiua-14101	191	4	,	,	PUNCT
aiua-14101	191	5	as	as	SCONJ
aiua-14101	191	6	sperm	sperm	NOUN
aiua-14101	191	7	concentration	concentration	NOUN
aiua-14101	191	8	is	be	AUX
aiua-14101	191	9	determined	determine	VERB
aiua-14101	191	10	prior	prior	ADV
aiua-14101	191	11	to	to	ADP
aiua-14101	191	12	freezing	freezing	NOUN
aiua-14101	191	13	and	and	CCONJ
aiua-14101	191	14	is	be	AUX
aiua-14101	191	15	used	use	VERB
aiua-14101	191	16	to	to	PART
aiua-14101	191	17	confirm	confirm	VERB
aiua-14101	191	18	the	the	DET
aiua-14101	191	19	amount	amount	NOUN
aiua-14101	191	20	of	of	ADP
aiua-14101	191	21	cells	cell	NOUN
aiua-14101	191	22	needed	need	VERB
aiua-14101	191	23	for	for	ADP
aiua-14101	191	24	post	post	ADJ
aiua-14101	191	25	-	-	ADJ
aiua-14101	191	26	freezethaw	freezethaw	ADJ
aiua-14101	191	27	analyses	analysis	NOUN
aiua-14101	191	28	and	and	CCONJ
aiua-14101	191	29	to	to	PART
aiua-14101	191	30	standardize	standardize	VERB
aiua-14101	191	31	experimental	experimental	ADJ
aiua-14101	191	32	procedures	procedure	NOUN
aiua-14101	191	33	(	(	PUNCT
aiua-14101	191	34	p	p	X
aiua-14101	191	35	>	>	X
aiua-14101	191	36	0.05	0.05	NUM
aiua-14101	191	37	)	)	PUNCT
aiua-14101	191	38	.	.	PUNCT
aiua-14101	192	1	when	when	SCONJ
aiua-14101	192	2	examining	examine	VERB
aiua-14101	192	3	the	the	DET
aiua-14101	192	4	abnormal	abnormal	ADJ
aiua-14101	192	5	acrosome	acrosome	NOUN
aiua-14101	192	6	ratio	ratio	NOUN
aiua-14101	192	7	,	,	PUNCT
aiua-14101	192	8	the	the	DET
aiua-14101	192	9	lowest	low	ADJ
aiua-14101	192	10	acrosome	acrosome	NOUN
aiua-14101	192	11	damage	damage	NOUN
aiua-14101	192	12	was	be	AUX
aiua-14101	192	13	found	find	VERB
aiua-14101	192	14	in	in	ADP
aiua-14101	192	15	the	the	DET
aiua-14101	192	16	trehalose	trehalose	NOUN
aiua-14101	192	17	(	(	PUNCT
aiua-14101	192	18	4.87	4.87	NUM
aiua-14101	192	19	±	±	NOUN
aiua-14101	192	20	0.63	0.63	NUM
aiua-14101	192	21	)	)	PUNCT
aiua-14101	192	22	and	and	CCONJ
aiua-14101	192	23	tryptophan	tryptophan	NOUN
aiua-14101	192	24	(	(	PUNCT
aiua-14101	192	25	4.75	4.75	NUM
aiua-14101	192	26	±	±	NUM
aiua-14101	192	27	0.75	0.75	NUM
aiua-14101	192	28	)	)	PUNCT
aiua-14101	192	29	groups	group	NOUN
aiua-14101	192	30	compared	compare	VERB
aiua-14101	192	31	to	to	ADP
aiua-14101	192	32	the	the	DET
aiua-14101	192	33	control	control	NOUN
aiua-14101	192	34	(	(	PUNCT
aiua-14101	192	35	6.37	6.37	NUM
aiua-14101	192	36	±	±	NUM
aiua-14101	192	37	0.99	0.99	NUM
aiua-14101	192	38	)	)	PUNCT
aiua-14101	192	39	,	,	PUNCT
aiua-14101	192	40	although	although	SCONJ
aiua-14101	192	41	the	the	DET
aiua-14101	192	42	differences	difference	NOUN
aiua-14101	192	43	were	be	AUX
aiua-14101	192	44	not	not	PART
aiua-14101	192	45	statistically	statistically	ADV
aiua-14101	192	46	significant	significant	ADJ
aiua-14101	192	47	(	(	PUNCT
aiua-14101	192	48	p	p	X
aiua-14101	192	49	>	>	X
aiua-14101	192	50	0.05	0.05	NUM
aiua-14101	192	51	)	)	PUNCT
aiua-14101	192	52	.	.	PUNCT
aiua-14101	193	1	these	these	DET
aiua-14101	193	2	findings	finding	NOUN
aiua-14101	193	3	indicate	indicate	VERB
aiua-14101	193	4	that	that	SCONJ
aiua-14101	193	5	trehalose	trehalose	NOUN
aiua-14101	193	6	and	and	CCONJ
aiua-14101	193	7	tryptophan	tryptophan	NOUN
aiua-14101	193	8	have	have	VERB
aiua-14101	193	9	comparable	comparable	ADJ
aiua-14101	193	10	cryoprotective	cryoprotective	ADJ
aiua-14101	193	11	effects	effect	NOUN
aiua-14101	193	12	in	in	ADP
aiua-14101	193	13	preserving	preserve	VERB
aiua-14101	193	14	acrosome	acrosome	NOUN
aiua-14101	193	15	integrity	integrity	NOUN
aiua-14101	193	16	.	.	PUNCT
aiua-14101	194	1	trehalose	trehalose	PROPN
aiua-14101	194	2	stabilizes	stabilize	VERB
aiua-14101	194	3	the	the	DET
aiua-14101	194	4	plasma	plasma	NOUN
aiua-14101	194	5	membrane	membrane	NOUN
aiua-14101	194	6	by	by	ADP
aiua-14101	194	7	reducing	reduce	VERB
aiua-14101	194	8	osmotic	osmotic	ADJ
aiua-14101	194	9	stress	stress	NOUN
aiua-14101	194	10	,	,	PUNCT
aiua-14101	194	11	while	while	SCONJ
aiua-14101	194	12	tryptophan	tryptophan	NOUN
aiua-14101	194	13	limits	limit	NOUN
aiua-14101	194	14	oxidative	oxidative	ADJ
aiua-14101	194	15	damage	damage	NOUN
aiua-14101	194	16	through	through	ADP
aiua-14101	194	17	its	its	PRON
aiua-14101	194	18	antioxidant	antioxidant	ADJ
aiua-14101	194	19	properties	property	NOUN
aiua-14101	194	20	.	.	PUNCT
aiua-14101	195	1	similar	similar	ADJ
aiua-14101	195	2	effects	effect	NOUN
aiua-14101	195	3	were	be	AUX
aiua-14101	195	4	reported	report	VERB
aiua-14101	195	5	by	by	ADP
aiua-14101	195	6	gholami	gholami	PROPN
aiua-14101	195	7	et	et	PROPN
aiua-14101	195	8	al	al	PROPN
aiua-14101	195	9	.	.	PUNCT
aiua-14101	196	1	(	(	PUNCT
aiua-14101	196	2	40	40	NUM
aiua-14101	196	3	)	)	PUNCT
aiua-14101	196	4	for	for	ADP
aiua-14101	196	5	trehalose	trehalose	PRON
aiua-14101	196	6	in	in	ADP
aiua-14101	196	7	ram	ram	NOUN
aiua-14101	196	8	sperm	sperm	NOUN
aiua-14101	196	9	,	,	PUNCT
aiua-14101	196	10	and	and	CCONJ
aiua-14101	196	11	by	by	ADP
aiua-14101	196	12	koçak	koçak	NOUN
aiua-14101	196	13	and	and	CCONJ
aiua-14101	196	14	yıldız	yıldız	PROPN
aiua-14101	196	15	(	(	PUNCT
aiua-14101	196	16	27	27	NUM
aiua-14101	196	17	)	)	PUNCT
aiua-14101	196	18	,	,	PUNCT
aiua-14101	196	19	who	who	PRON
aiua-14101	196	20	showed	show	VERB
aiua-14101	196	21	that	that	SCONJ
aiua-14101	196	22	25	25	NUM
aiua-14101	196	23	mm	mm	NOUN
aiua-14101	196	24	tryptophan	tryptophan	NOUN
aiua-14101	196	25	reduced	reduce	VERB
aiua-14101	196	26	acrosome	acrosome	NOUN
aiua-14101	196	27	damage	damage	NOUN
aiua-14101	196	28	in	in	ADP
aiua-14101	196	29	mouse	mouse	NOUN
aiua-14101	196	30	sperm	sperm	NOUN
aiua-14101	196	31	(	(	PUNCT
aiua-14101	196	32	11.17	11.17	NUM
aiua-14101	196	33	±	±	NUM
aiua-14101	196	34	1.22	1.22	NUM
aiua-14101	196	35	)	)	PUNCT
aiua-14101	196	36	compared	compare	VERB
aiua-14101	196	37	to	to	ADP
aiua-14101	196	38	the	the	DET
aiua-14101	196	39	control	control	NOUN
aiua-14101	196	40	(	(	PUNCT
aiua-14101	196	41	12.83	12.83	NUM
aiua-14101	196	42	±	±	NUM
aiua-14101	196	43	1.22	1.22	NUM
aiua-14101	196	44	)	)	PUNCT
aiua-14101	196	45	.	.	PUNCT
aiua-14101	197	1	the	the	DET
aiua-14101	197	2	comparable	comparable	ADJ
aiua-14101	197	3	outcomes	outcome	NOUN
aiua-14101	197	4	in	in	ADP
aiua-14101	197	5	our	our	PRON
aiua-14101	197	6	study	study	NOUN
aiua-14101	197	7	suggest	suggest	VERB
aiua-14101	197	8	that	that	SCONJ
aiua-14101	197	9	both	both	DET
aiua-14101	197	10	additives	additive	NOUN
aiua-14101	197	11	have	have	VERB
aiua-14101	197	12	potential	potential	NOUN
aiua-14101	197	13	to	to	PART
aiua-14101	197	14	reduce	reduce	VERB
aiua-14101	197	15	acrosomal	acrosomal	NOUN
aiua-14101	197	16	damage	damage	NOUN
aiua-14101	197	17	,	,	PUNCT
aiua-14101	197	18	but	but	CCONJ
aiua-14101	197	19	larger	large	ADJ
aiua-14101	197	20	sample	sample	NOUN
aiua-14101	197	21	sizes	size	NOUN
aiua-14101	197	22	may	may	AUX
aiua-14101	197	23	be	be	AUX
aiua-14101	197	24	needed	need	VERB
aiua-14101	197	25	to	to	PART
aiua-14101	197	26	reveal	reveal	VERB
aiua-14101	197	27	significant	significant	ADJ
aiua-14101	197	28	differences	difference	NOUN
aiua-14101	197	29	.	.	PUNCT
aiua-14101	198	1	apoptosis	apoptosis	NOUN
aiua-14101	198	2	is	be	AUX
aiua-14101	198	3	a	a	DET
aiua-14101	198	4	mechanism	mechanism	NOUN
aiua-14101	198	5	of	of	ADP
aiua-14101	198	6	programmed	program	VERB
aiua-14101	198	7	cell	cell	NOUN
aiua-14101	198	8	death	death	NOUN
aiua-14101	198	9	,	,	PUNCT
aiua-14101	198	10	being	be	AUX
aiua-14101	198	11	a	a	DET
aiua-14101	198	12	physiological	physiological	ADJ
aiua-14101	198	13	process	process	NOUN
aiua-14101	198	14	that	that	PRON
aiua-14101	198	15	eliminates	eliminate	VERB
aiua-14101	198	16	cells	cell	NOUN
aiua-14101	198	17	that	that	PRON
aiua-14101	198	18	threaten	threaten	VERB
aiua-14101	198	19	survival	survival	NOUN
aiua-14101	198	20	,	,	PUNCT
aiua-14101	198	21	and	and	CCONJ
aiua-14101	198	22	is	be	AUX
aiua-14101	198	23	critical	critical	ADJ
aiua-14101	198	24	for	for	ADP
aiua-14101	198	25	spermatozoa	spermatozoa	NOUN
aiua-14101	198	26	(	(	PUNCT
aiua-14101	198	27	16	16	NUM
aiua-14101	198	28	)	)	PUNCT
aiua-14101	198	29	.	.	PUNCT
aiua-14101	199	1	therefore	therefore	ADV
aiua-14101	199	2	,	,	PUNCT
aiua-14101	199	3	in	in	ADP
aiua-14101	199	4	this	this	DET
aiua-14101	199	5	study	study	NOUN
aiua-14101	199	6	,	,	PUNCT
aiua-14101	199	7	the	the	DET
aiua-14101	199	8	annexin	annexin	NOUN
aiua-14101	199	9	v	v	NOUN
aiua-14101	199	10	-	-	PUNCT
aiua-14101	199	11	fitc	fitc	NOUN
aiua-14101	199	12	/	/	SYM
aiua-14101	199	13	pi	pi	NOUN
aiua-14101	199	14	test	test	NOUN
aiua-14101	199	15	was	be	AUX
aiua-14101	199	16	performed	perform	VERB
aiua-14101	199	17	to	to	PART
aiua-14101	199	18	determine	determine	VERB
aiua-14101	199	19	whether	whether	SCONJ
aiua-14101	199	20	tryptophan	tryptophan	NOUN
aiua-14101	199	21	and	and	CCONJ
aiua-14101	199	22	trehalose	trehalose	PRON
aiua-14101	199	23	added	add	VERB
aiua-14101	199	24	to	to	ADP
aiua-14101	199	25	the	the	DET
aiua-14101	199	26	cryopreservation	cryopreservation	NOUN
aiua-14101	199	27	diluent	diluent	NOUN
aiua-14101	199	28	had	have	VERB
aiua-14101	199	29	any	any	DET
aiua-14101	199	30	effect	effect	NOUN
aiua-14101	199	31	on	on	ADP
aiua-14101	199	32	the	the	DET
aiua-14101	199	33	apoptotic	apoptotic	ADJ
aiua-14101	199	34	mechanism	mechanism	NOUN
aiua-14101	199	35	after	after	ADP
aiua-14101	199	36	thawing	thaw	VERB
aiua-14101	199	37	.	.	PUNCT
aiua-14101	200	1	according	accord	VERB
aiua-14101	200	2	to	to	ADP
aiua-14101	200	3	the	the	DET
aiua-14101	200	4	test	test	NOUN
aiua-14101	200	5	results	result	NOUN
aiua-14101	200	6	,	,	PUNCT
aiua-14101	200	7	spermatozoa	spermatozoon	NOUN
aiua-14101	200	8	with	with	ADP
aiua-14101	200	9	the	the	DET
aiua-14101	200	10	head	head	NOUN
aiua-14101	200	11	stained	stain	VERB
aiua-14101	200	12	completely	completely	ADV
aiua-14101	200	13	green	green	ADJ
aiua-14101	200	14	were	be	AUX
aiua-14101	200	15	considered	consider	VERB
aiua-14101	200	16	as	as	ADP
aiua-14101	200	17	early	early	ADJ
aiua-14101	200	18	apoptotic	apoptotic	ADJ
aiua-14101	200	19	,	,	PUNCT
aiua-14101	200	20	spermatozoa	spermatozoon	NOUN
aiua-14101	200	21	with	with	ADP
aiua-14101	200	22	green	green	ADJ
aiua-14101	200	23	-	-	PUNCT
aiua-14101	200	24	red	red	ADJ
aiua-14101	200	25	staining	stain	VERB
aiua-14101	200	26	were	be	AUX
aiua-14101	200	27	considered	consider	VERB
aiua-14101	200	28	as	as	ADP
aiua-14101	200	29	late	late	ADJ
aiua-14101	200	30	apoptotic	apoptotic	ADJ
aiua-14101	200	31	,	,	PUNCT
aiua-14101	200	32	and	and	CCONJ
aiua-14101	200	33	spermatozoa	spermatozoon	NOUN
aiua-14101	200	34	with	with	ADP
aiua-14101	200	35	red	red	ADJ
aiua-14101	200	36	staining	staining	NOUN
aiua-14101	200	37	were	be	AUX
aiua-14101	200	38	considered	consider	VERB
aiua-14101	200	39	as	as	ADP
aiua-14101	200	40	necrotic	necrotic	ADJ
aiua-14101	200	41	.	.	PUNCT
aiua-14101	201	1	when	when	SCONJ
aiua-14101	201	2	apoptotic	apoptotic	ADJ
aiua-14101	201	3	cell	cell	NOUN
aiua-14101	201	4	ratios	ratio	NOUN
aiua-14101	201	5	were	be	AUX
aiua-14101	201	6	evaluated	evaluate	VERB
aiua-14101	201	7	together	together	ADV
aiua-14101	201	8	with	with	ADP
aiua-14101	201	9	apoptosis	apoptosis	NOUN
aiua-14101	201	10	-	-	PUNCT
aiua-14101	201	11	related	relate	VERB
aiua-14101	201	12	gene	gene	NOUN
aiua-14101	201	13	expression	expression	NOUN
aiua-14101	201	14	,	,	PUNCT
aiua-14101	201	15	trehalose	trehalose	PROPN
aiua-14101	201	16	demonstrated	demonstrate	VERB
aiua-14101	201	17	a	a	DET
aiua-14101	201	18	clear	clear	ADJ
aiua-14101	201	19	cryoprotective	cryoprotective	ADJ
aiua-14101	201	20	advantage	advantage	NOUN
aiua-14101	201	21	.	.	PUNCT
aiua-14101	202	1	the	the	DET
aiua-14101	202	2	annexin	annexin	NOUN
aiua-14101	202	3	v	v	NOUN
aiua-14101	202	4	-	-	PUNCT
aiua-14101	202	5	fitc	fitc	NOUN
aiua-14101	202	6	/	/	SYM
aiua-14101	202	7	pi	pi	NOUN
aiua-14101	202	8	assay	assay	PROPN
aiua-14101	202	9	demonstrated	demonstrate	VERB
aiua-14101	202	10	a	a	DET
aiua-14101	202	11	significantly	significantly	ADV
aiua-14101	202	12	reduced	reduce	VERB
aiua-14101	202	13	apoptosis	apoptosis	NOUN
aiua-14101	202	14	index	index	NOUN
aiua-14101	202	15	(	(	PUNCT
aiua-14101	202	16	%	%	INTJ
aiua-14101	202	17	)	)	PUNCT
aiua-14101	202	18	in	in	ADP
aiua-14101	202	19	the	the	DET
aiua-14101	202	20	trehalose	trehalose	PROPN
aiua-14101	202	21	group	group	NOUN
aiua-14101	202	22	(	(	PUNCT
aiua-14101	202	23	22.37	22.37	NUM
aiua-14101	202	24	±	±	NUM
aiua-14101	202	25	2.94	2.94	NUM
aiua-14101	202	26	)	)	PUNCT
aiua-14101	202	27	compared	compare	VERB
aiua-14101	202	28	with	with	ADP
aiua-14101	202	29	controls	control	NOUN
aiua-14101	202	30	(	(	PUNCT
aiua-14101	202	31	33.00	33.00	NUM
aiua-14101	202	32	±	±	NUM
aiua-14101	202	33	4.55	4.55	NUM
aiua-14101	202	34	,	,	PUNCT
aiua-14101	202	35	p	p	X
aiua-14101	202	36	<	<	X
aiua-14101	202	37	0.01	0.01	NUM
aiua-14101	202	38	)	)	PUNCT
aiua-14101	202	39	,	,	PUNCT
aiua-14101	202	40	aligning	align	VERB
aiua-14101	202	41	with	with	ADP
aiua-14101	202	42	the	the	DET
aiua-14101	202	43	downregulation	downregulation	NOUN
aiua-14101	202	44	of	of	ADP
aiua-14101	202	45	proapoptotic	proapoptotic	ADJ
aiua-14101	202	46	bax	bax	PROPN
aiua-14101	202	47	(	(	PUNCT
aiua-14101	202	48	p	p	X
aiua-14101	202	49	<	<	X
aiua-14101	202	50	0.001	0.001	NUM
aiua-14101	202	51	)	)	PUNCT
aiua-14101	202	52	and	and	CCONJ
aiua-14101	202	53	caspase-3	caspase-3	NUM
aiua-14101	202	54	(	(	PUNCT
aiua-14101	202	55	p	p	X
aiua-14101	202	56	<	<	X
aiua-14101	202	57	0.05	0.05	NUM
aiua-14101	202	58	)	)	PUNCT
aiua-14101	202	59	expression	expression	NOUN
aiua-14101	202	60	and	and	CCONJ
aiua-14101	202	61	the	the	DET
aiua-14101	202	62	upregulation	upregulation	NOUN
aiua-14101	202	63	of	of	ADP
aiua-14101	202	64	anti	anti	ADJ
aiua-14101	202	65	-	-	ADJ
aiua-14101	202	66	apoptotic	apoptotic	ADJ
aiua-14101	202	67	bcl-2	bcl-2	ADJ
aiua-14101	202	68	expression	expression	NOUN
aiua-14101	202	69	(	(	PUNCT
aiua-14101	202	70	p	p	X
aiua-14101	202	71	<	<	X
aiua-14101	202	72	0.001	0.001	NUM
aiua-14101	202	73	)	)	PUNCT
aiua-14101	202	74	.	.	PUNCT
aiua-14101	203	1	these	these	DET
aiua-14101	203	2	findings	finding	NOUN
aiua-14101	203	3	are	be	AUX
aiua-14101	203	4	consistent	consistent	ADJ
aiua-14101	203	5	with	with	ADP
aiua-14101	203	6	previous	previous	ADJ
aiua-14101	203	7	studies	study	NOUN
aiua-14101	203	8	(	(	PUNCT
aiua-14101	203	9	41	41	NUM
aiua-14101	203	10	-	-	SYM
aiua-14101	203	11	44	44	NUM
aiua-14101	203	12	)	)	PUNCT
aiua-14101	203	13	.	.	PUNCT
aiua-14101	204	1	in	in	ADP
aiua-14101	204	2	canine	canine	NOUN
aiua-14101	204	3	sperm	sperm	NOUN
aiua-14101	204	4	,	,	PUNCT
aiua-14101	204	5	trehalose	trehalose	ADJ
aiua-14101	204	6	supplementation	supplementation	NOUN
aiua-14101	204	7	during	during	ADP
aiua-14101	204	8	cryopreservation	cryopreservation	NOUN
aiua-14101	204	9	caused	cause	VERB
aiua-14101	204	10	slight	slight	ADJ
aiua-14101	204	11	numerical	numerical	ADJ
aiua-14101	204	12	increases	increase	NOUN
aiua-14101	204	13	in	in	ADP
aiua-14101	204	14	bcl-2	bcl-2	NOUN
aiua-14101	204	15	and	and	CCONJ
aiua-14101	204	16	decreases	decrease	NOUN
aiua-14101	204	17	in	in	ADP
aiua-14101	204	18	bax	bax	NOUN
aiua-14101	204	19	expression	expression	NOUN
aiua-14101	204	20	(	(	PUNCT
aiua-14101	204	21	45	45	NUM
aiua-14101	204	22	)	)	PUNCT
aiua-14101	204	23	.	.	PUNCT
aiua-14101	205	1	similarly	similarly	ADV
aiua-14101	205	2	,	,	PUNCT
aiua-14101	205	3	in	in	ADP
aiua-14101	205	4	rat	rat	NOUN
aiua-14101	205	5	ovaries	ovary	NOUN
aiua-14101	205	6	,	,	PUNCT
aiua-14101	205	7	xie	xie	PROPN
aiua-14101	205	8	et	et	PROPN
aiua-14101	205	9	al	al	PROPN
aiua-14101	205	10	.	.	PROPN
aiua-14101	206	1	(	(	PUNCT
aiua-14101	206	2	41	41	NUM
aiua-14101	206	3	)	)	PUNCT
aiua-14101	206	4	reported	report	VERB
aiua-14101	206	5	that	that	SCONJ
aiua-14101	206	6	adding	add	VERB
aiua-14101	206	7	trehalose	trehalose	PRON
aiua-14101	206	8	to	to	ADP
aiua-14101	206	9	the	the	DET
aiua-14101	206	10	cryoprotective	cryoprotective	ADJ
aiua-14101	206	11	agent	agent	NOUN
aiua-14101	206	12	significantly	significantly	ADV
aiua-14101	206	13	reduced	reduce	VERB
aiua-14101	206	14	the	the	DET
aiua-14101	206	15	apoptotic	apoptotic	ADJ
aiua-14101	206	16	cell	cell	NOUN
aiua-14101	206	17	ratio	ratio	NOUN
aiua-14101	206	18	and	and	CCONJ
aiua-14101	206	19	suppressed	suppress	VERB
aiua-14101	206	20	bax	bax	NOUN
aiua-14101	206	21	gene	gene	NOUN
aiua-14101	206	22	expression	expression	NOUN
aiua-14101	206	23	.	.	PUNCT
aiua-14101	207	1	however	however	ADV
aiua-14101	207	2	,	,	PUNCT
aiua-14101	207	3	bumbat	bumbat	PROPN
aiua-14101	207	4	et	et	PROPN
aiua-14101	207	5	al	al	PROPN
aiua-14101	207	6	.	.	PUNCT
aiua-14101	208	1	(	(	PUNCT
aiua-14101	208	2	46	46	NUM
aiua-14101	208	3	)	)	PUNCT
aiua-14101	208	4	reported	report	VERB
aiua-14101	208	5	that	that	SCONJ
aiua-14101	208	6	trehalose	trehalose	ADJ
aiua-14101	208	7	supplementation	supplementation	NOUN
aiua-14101	208	8	in	in	ADP
aiua-14101	208	9	the	the	DET
aiua-14101	208	10	cryopreservation	cryopreservation	NOUN
aiua-14101	208	11	archivio	archivio	NOUN
aiua-14101	208	12	italiano	italiano	X
aiua-14101	208	13	di	di	X
aiua-14101	208	14	urologia	urologia	PROPN
aiua-14101	208	15	e	e	PROPN
aiua-14101	208	16	andrologia	andrologia	NOUN
aiua-14101	208	17	2025	2025	NUM
aiua-14101	208	18	;	;	PUNCT
aiua-14101	208	19	97(3):14101	97(3):14101	NUM
aiua-14101	208	20	7	7	NUM
aiua-14101	208	21	tryptophan	tryptophan	NOUN
aiua-14101	208	22	and	and	CCONJ
aiua-14101	208	23	trehalose	trehalose	NOUN
aiua-14101	208	24	on	on	ADP
aiua-14101	208	25	post	post	ADJ
aiua-14101	208	26	-	-	ADJ
aiua-14101	208	27	thaw	thaw	ADJ
aiua-14101	208	28	sperm	sperm	NOUN
aiua-14101	208	29	medium	medium	NOUN
aiua-14101	208	30	of	of	ADP
aiua-14101	208	31	cancer	cancer	NOUN
aiua-14101	208	32	cells	cell	NOUN
aiua-14101	208	33	paradoxically	paradoxically	ADV
aiua-14101	208	34	upregulated	upregulate	VERB
aiua-14101	208	35	not	not	PART
aiua-14101	208	36	only	only	ADV
aiua-14101	208	37	the	the	DET
aiua-14101	208	38	anti	anti	ADJ
aiua-14101	208	39	-	-	ADJ
aiua-14101	208	40	apoptotic	apoptotic	ADJ
aiua-14101	208	41	gene	gene	NOUN
aiua-14101	208	42	bcl-2	bcl-2	PUNCT
aiua-14101	208	43	but	but	CCONJ
aiua-14101	208	44	also	also	ADV
aiua-14101	208	45	the	the	DET
aiua-14101	208	46	pro	pro	ADJ
aiua-14101	208	47	-	-	ADJ
aiua-14101	208	48	apoptotic	apoptotic	ADJ
aiua-14101	208	49	genes	gene	NOUN
aiua-14101	208	50	bax	bax	PROPN
aiua-14101	208	51	and	and	CCONJ
aiua-14101	208	52	bad	bad	ADJ
aiua-14101	208	53	,	,	PUNCT
aiua-14101	208	54	suggesting	suggest	VERB
aiua-14101	208	55	that	that	SCONJ
aiua-14101	208	56	such	such	ADJ
aiua-14101	208	57	variations	variation	NOUN
aiua-14101	208	58	may	may	AUX
aiua-14101	208	59	depend	depend	VERB
aiua-14101	208	60	on	on	ADP
aiua-14101	208	61	tissue	tissue	NOUN
aiua-14101	208	62	type	type	NOUN
aiua-14101	208	63	or	or	CCONJ
aiua-14101	208	64	experimental	experimental	ADJ
aiua-14101	208	65	conditions	condition	NOUN
aiua-14101	208	66	.	.	PUNCT
aiua-14101	209	1	overall	overall	ADV
aiua-14101	209	2	,	,	PUNCT
aiua-14101	209	3	trehalose	trehalose	PRON
aiua-14101	209	4	consistently	consistently	ADV
aiua-14101	209	5	reduced	reduce	VERB
aiua-14101	209	6	both	both	PRON
aiua-14101	209	7	apoptotic	apoptotic	ADJ
aiua-14101	209	8	cell	cell	NOUN
aiua-14101	209	9	counts	count	NOUN
aiua-14101	209	10	and	and	CCONJ
aiua-14101	209	11	pro	pro	ADJ
aiua-14101	209	12	-	-	ADJ
aiua-14101	209	13	apoptotic	apoptotic	ADJ
aiua-14101	209	14	gene	gene	NOUN
aiua-14101	209	15	expression	expression	NOUN
aiua-14101	209	16	,	,	PUNCT
aiua-14101	209	17	whereas	whereas	SCONJ
aiua-14101	209	18	tryptophan	tryptophan	NOUN
aiua-14101	209	19	showed	show	VERB
aiua-14101	209	20	more	more	ADJ
aiua-14101	209	21	variable	variable	ADJ
aiua-14101	209	22	effects	effect	NOUN
aiua-14101	209	23	.	.	PUNCT
aiua-14101	210	1	although	although	SCONJ
aiua-14101	210	2	tryptophan	tryptophan	NOUN
aiua-14101	210	3	also	also	ADV
aiua-14101	210	4	lowered	lower	VERB
aiua-14101	210	5	bax	bax	PROPN
aiua-14101	210	6	and	and	CCONJ
aiua-14101	210	7	caspase-3	caspase-3	NUM
aiua-14101	210	8	expression	expression	NOUN
aiua-14101	210	9	,	,	PUNCT
aiua-14101	210	10	the	the	DET
aiua-14101	210	11	apoptosis	apoptosis	NOUN
aiua-14101	210	12	index	index	NOUN
aiua-14101	210	13	in	in	ADP
aiua-14101	210	14	the	the	DET
aiua-14101	210	15	tryptophan	tryptophan	NOUN
aiua-14101	210	16	group	group	NOUN
aiua-14101	210	17	(	(	PUNCT
aiua-14101	210	18	55.00	55.00	NUM
aiua-14101	210	19	±	±	NUM
aiua-14101	210	20	3.95	3.95	NUM
aiua-14101	210	21	)	)	PUNCT
aiua-14101	210	22	was	be	AUX
aiua-14101	210	23	unexpectedly	unexpectedly	ADV
aiua-14101	210	24	higher	high	ADJ
aiua-14101	210	25	than	than	ADP
aiua-14101	210	26	in	in	ADP
aiua-14101	210	27	the	the	DET
aiua-14101	210	28	control	control	NOUN
aiua-14101	210	29	,	,	PUNCT
aiua-14101	210	30	suggesting	suggest	VERB
aiua-14101	210	31	that	that	SCONJ
aiua-14101	210	32	the	the	DET
aiua-14101	210	33	25	25	NUM
aiua-14101	210	34	mm	mm	NOUN
aiua-14101	210	35	concentration	concentration	NOUN
aiua-14101	210	36	may	may	AUX
aiua-14101	210	37	not	not	PART
aiua-14101	210	38	be	be	AUX
aiua-14101	210	39	optimal	optimal	ADJ
aiua-14101	210	40	and	and	CCONJ
aiua-14101	210	41	that	that	SCONJ
aiua-14101	210	42	its	its	PRON
aiua-14101	210	43	effects	effect	NOUN
aiua-14101	210	44	could	could	AUX
aiua-14101	210	45	be	be	AUX
aiua-14101	210	46	dose	dose	VERB
aiua-14101	210	47	-	-	PUNCT
aiua-14101	210	48	dependent	dependent	ADJ
aiua-14101	210	49	,	,	PUNCT
aiua-14101	210	50	as	as	SCONJ
aiua-14101	210	51	reported	report	VERB
aiua-14101	210	52	in	in	ADP
aiua-14101	210	53	several	several	ADJ
aiua-14101	210	54	sperm	sperm	NOUN
aiua-14101	210	55	cryopreservation	cryopreservation	NOUN
aiua-14101	210	56	studies	study	NOUN
aiua-14101	210	57	(	(	PUNCT
aiua-14101	210	58	27	27	NUM
aiua-14101	210	59	,	,	PUNCT
aiua-14101	210	60	34	34	NUM
aiua-14101	210	61	,	,	PUNCT
aiua-14101	210	62	36	36	NUM
aiua-14101	210	63	)	)	PUNCT
aiua-14101	210	64	.	.	PUNCT
aiua-14101	211	1	in	in	ADP
aiua-14101	211	2	summary	summary	NOUN
aiua-14101	211	3	,	,	PUNCT
aiua-14101	211	4	trehalose	trehalose	PRON
aiua-14101	211	5	provided	provide	VERB
aiua-14101	211	6	more	more	ADV
aiua-14101	211	7	consistent	consistent	ADJ
aiua-14101	211	8	cryoprotective	cryoprotective	ADJ
aiua-14101	211	9	benefits	benefit	NOUN
aiua-14101	211	10	than	than	ADP
aiua-14101	211	11	tryptophan	tryptophan	NOUN
aiua-14101	211	12	by	by	ADP
aiua-14101	211	13	improving	improve	VERB
aiua-14101	211	14	viability	viability	NOUN
aiua-14101	211	15	,	,	PUNCT
aiua-14101	211	16	preserving	preserve	VERB
aiua-14101	211	17	plasma	plasma	NOUN
aiua-14101	211	18	membrane	membrane	NOUN
aiua-14101	211	19	integrity	integrity	NOUN
aiua-14101	211	20	,	,	PUNCT
aiua-14101	211	21	reducing	reduce	VERB
aiua-14101	211	22	apoptosis	apoptosis	NOUN
aiua-14101	211	23	,	,	PUNCT
aiua-14101	211	24	and	and	CCONJ
aiua-14101	211	25	maintaining	maintain	VERB
aiua-14101	211	26	acrosomal	acrosomal	NOUN
aiua-14101	211	27	structure	structure	NOUN
aiua-14101	211	28	.	.	PUNCT
aiua-14101	212	1	tryptophan	tryptophan	NOUN
aiua-14101	212	2	offered	offer	VERB
aiua-14101	212	3	limited	limited	ADJ
aiua-14101	212	4	benefits	benefit	NOUN
aiua-14101	212	5	,	,	PUNCT
aiua-14101	212	6	mainly	mainly	ADV
aiua-14101	212	7	through	through	ADP
aiua-14101	212	8	its	its	PRON
aiua-14101	212	9	antioxidant	antioxidant	ADJ
aiua-14101	212	10	action	action	NOUN
aiua-14101	212	11	,	,	PUNCT
aiua-14101	212	12	and	and	CCONJ
aiua-14101	212	13	its	its	PRON
aiua-14101	212	14	effectiveness	effectiveness	NOUN
aiua-14101	212	15	may	may	AUX
aiua-14101	212	16	vary	vary	VERB
aiua-14101	212	17	with	with	ADP
aiua-14101	212	18	concentration	concentration	NOUN
aiua-14101	212	19	.	.	PUNCT
aiua-14101	213	1	these	these	DET
aiua-14101	213	2	findings	finding	NOUN
aiua-14101	213	3	indicate	indicate	VERB
aiua-14101	213	4	that	that	SCONJ
aiua-14101	213	5	optimizing	optimize	VERB
aiua-14101	213	6	trehalose	trehalose	DET
aiua-14101	213	7	concentration	concentration	NOUN
aiua-14101	213	8	is	be	AUX
aiua-14101	213	9	a	a	DET
aiua-14101	213	10	promising	promising	ADJ
aiua-14101	213	11	approach	approach	NOUN
aiua-14101	213	12	for	for	ADP
aiua-14101	213	13	rat	rat	NOUN
aiua-14101	213	14	sperm	sperm	NOUN
aiua-14101	213	15	cryopreservation	cryopreservation	NOUN
aiua-14101	213	16	,	,	PUNCT
aiua-14101	213	17	while	while	SCONJ
aiua-14101	213	18	further	further	ADJ
aiua-14101	213	19	research	research	NOUN
aiua-14101	213	20	is	be	AUX
aiua-14101	213	21	needed	need	VERB
aiua-14101	213	22	to	to	PART
aiua-14101	213	23	explore	explore	VERB
aiua-14101	213	24	the	the	DET
aiua-14101	213	25	dose	dose	NOUN
aiua-14101	213	26	-	-	PUNCT
aiua-14101	213	27	response	response	NOUN
aiua-14101	213	28	effects	effect	NOUN
aiua-14101	213	29	of	of	ADP
aiua-14101	213	30	tryptophan	tryptophan	NOUN
aiua-14101	213	31	and	and	CCONJ
aiua-14101	213	32	to	to	PART
aiua-14101	213	33	evaluate	evaluate	VERB
aiua-14101	213	34	fertility	fertility	NOUN
aiua-14101	213	35	outcomes	outcome	NOUN
aiua-14101	213	36	in	in	ADP
aiua-14101	213	37	vivo	vivo	NOUN
aiua-14101	213	38	.	.	PUNCT
aiua-14101	214	1	conclusions	conclusion	NOUN
aiua-14101	214	2	in	in	ADP
aiua-14101	214	3	conclusion	conclusion	NOUN
aiua-14101	214	4	,	,	PUNCT
aiua-14101	214	5	according	accord	VERB
aiua-14101	214	6	to	to	ADP
aiua-14101	214	7	the	the	DET
aiua-14101	214	8	parameters	parameter	NOUN
aiua-14101	214	9	evaluated	evaluate	VERB
aiua-14101	214	10	,	,	PUNCT
aiua-14101	214	11	the	the	DET
aiua-14101	214	12	addition	addition	NOUN
aiua-14101	214	13	of	of	ADP
aiua-14101	214	14	the	the	DET
aiua-14101	214	15	disaccharide	disaccharide	NOUN
aiua-14101	214	16	sugar	sugar	NOUN
aiua-14101	214	17	trehalose	trehalose	ADV
aiua-14101	214	18	to	to	ADP
aiua-14101	214	19	rat	rat	NOUN
aiua-14101	214	20	sperm	sperm	NOUN
aiua-14101	214	21	diluent	diluent	NOUN
aiua-14101	214	22	provided	provide	VERB
aiua-14101	214	23	more	more	ADV
aiua-14101	214	24	effective	effective	ADJ
aiua-14101	214	25	protection	protection	NOUN
aiua-14101	214	26	against	against	ADP
aiua-14101	214	27	cryodamage	cryodamage	NOUN
aiua-14101	214	28	during	during	ADP
aiua-14101	214	29	cryopreservation	cryopreservation	NOUN
aiua-14101	214	30	than	than	ADP
aiua-14101	214	31	the	the	DET
aiua-14101	214	32	aromatic	aromatic	ADJ
aiua-14101	214	33	amino	amino	NOUN
aiua-14101	214	34	acid	acid	NOUN
aiua-14101	214	35	tryptophan	tryptophan	NOUN
aiua-14101	214	36	.	.	PUNCT
aiua-14101	215	1	the	the	DET
aiua-14101	215	2	use	use	NOUN
aiua-14101	215	3	of	of	ADP
aiua-14101	215	4	these	these	DET
aiua-14101	215	5	substances	substance	NOUN
aiua-14101	215	6	may	may	AUX
aiua-14101	215	7	provide	provide	VERB
aiua-14101	215	8	a	a	DET
aiua-14101	215	9	significant	significant	ADJ
aiua-14101	215	10	advance	advance	NOUN
aiua-14101	215	11	in	in	ADP
aiua-14101	215	12	overcoming	overcome	VERB
aiua-14101	215	13	the	the	DET
aiua-14101	215	14	difficulties	difficulty	NOUN
aiua-14101	215	15	encountered	encounter	VERB
aiua-14101	215	16	in	in	ADP
aiua-14101	215	17	the	the	DET
aiua-14101	215	18	cryopreservation	cryopreservation	NOUN
aiua-14101	215	19	process	process	NOUN
aiua-14101	215	20	of	of	ADP
aiua-14101	215	21	rat	rat	NOUN
aiua-14101	215	22	sperm	sperm	NOUN
aiua-14101	215	23	and	and	CCONJ
aiua-14101	215	24	may	may	AUX
aiua-14101	215	25	lay	lay	VERB
aiua-14101	215	26	a	a	DET
aiua-14101	215	27	strong	strong	ADJ
aiua-14101	215	28	foundation	foundation	NOUN
aiua-14101	215	29	for	for	ADP
aiua-14101	215	30	future	future	ADJ
aiua-14101	215	31	studies	study	NOUN
aiua-14101	215	32	.	.	PUNCT
aiua-14101	216	1	references	reference	NOUN
aiua-14101	216	2	1	1	NUM
aiua-14101	216	3	.	.	PUNCT
aiua-14101	217	1	takeo	takeo	PROPN
aiua-14101	217	2	t	t	PROPN
aiua-14101	217	3	,	,	PUNCT
aiua-14101	217	4	nakao	nakao	PROPN
aiua-14101	217	5	s	s	PROPN
aiua-14101	217	6	,	,	PUNCT
aiua-14101	217	7	mikoda	mikoda	NOUN
aiua-14101	217	8	n	n	CCONJ
aiua-14101	217	9	,	,	PUNCT
aiua-14101	217	10	et	et	PROPN
aiua-14101	217	11	al	al	PROPN
aiua-14101	217	12	.	.	PROPN
aiua-14101	217	13	optimized	optimize	VERB
aiua-14101	217	14	protocols	protocol	NOUN
aiua-14101	217	15	for	for	ADP
aiua-14101	217	16	sperm	sperm	NOUN
aiua-14101	217	17	cryopreservation	cryopreservation	NOUN
aiua-14101	217	18	and	and	CCONJ
aiua-14101	217	19	in	in	ADP
aiua-14101	217	20	vitro	vitro	X
aiua-14101	217	21	fertilization	fertilization	NOUN
aiua-14101	217	22	in	in	ADP
aiua-14101	217	23	the	the	DET
aiua-14101	217	24	rat	rat	NOUN
aiua-14101	217	25	.	.	PROPN
aiua-14101	217	26	lab	lab	PROPN
aiua-14101	217	27	anim	anim	NOUN
aiua-14101	217	28	.	.	PUNCT
aiua-14101	217	29	2022	2022	NUM
aiua-14101	217	30	;	;	PUNCT
aiua-14101	217	31	51:256	51:256	NUM
aiua-14101	217	32	-	-	SYM
aiua-14101	217	33	74	74	NUM
aiua-14101	217	34	.	.	PUNCT
aiua-14101	218	1	2	2	NUM
aiua-14101	218	2	.	.	X
aiua-14101	218	3	nakatsukasa	nakatsukasa	NOUN
aiua-14101	218	4	e	e	NOUN
aiua-14101	218	5	,	,	PUNCT
aiua-14101	218	6	kashiwazaki	kashiwazaki	PROPN
aiua-14101	218	7	n	n	CCONJ
aiua-14101	218	8	,	,	PUNCT
aiua-14101	218	9	takizawa	takizawa	PROPN
aiua-14101	218	10	a	a	PROPN
aiua-14101	218	11	,	,	PUNCT
aiua-14101	218	12	et	et	PROPN
aiua-14101	218	13	al	al	PROPN
aiua-14101	218	14	.	.	PUNCT
aiua-14101	218	15	cryopreservation	cryopreservation	NOUN
aiua-14101	218	16	of	of	ADP
aiua-14101	218	17	spermatozoa	spermatozoon	NOUN
aiua-14101	218	18	from	from	ADP
aiua-14101	218	19	closed	closed	ADJ
aiua-14101	218	20	colonies	colony	NOUN
aiua-14101	218	21	,	,	PUNCT
aiua-14101	218	22	and	and	CCONJ
aiua-14101	218	23	inbred	inbreed	VERB
aiua-14101	218	24	,	,	PUNCT
aiua-14101	218	25	spontaneous	spontaneous	ADJ
aiua-14101	218	26	mutant	mutant	NOUN
aiua-14101	218	27	,	,	PUNCT
aiua-14101	218	28	and	and	CCONJ
aiua-14101	218	29	transgenic	transgenic	NOUN
aiua-14101	218	30	strains	strain	NOUN
aiua-14101	218	31	of	of	ADP
aiua-14101	218	32	rats	rat	NOUN
aiua-14101	218	33	.	.	PUNCT
aiua-14101	219	1	comp	comp	PROPN
aiua-14101	219	2	med	med	PROPN
aiua-14101	219	3	.	.	PUNCT
aiua-14101	219	4	2003	2003	NUM
aiua-14101	219	5	;	;	PUNCT
aiua-14101	219	6	53:639	53:639	NUM
aiua-14101	219	7	-	-	SYM
aiua-14101	219	8	46	46	NUM
aiua-14101	219	9	.	.	PUNCT
aiua-14101	220	1	3	3	X
aiua-14101	220	2	.	.	X
aiua-14101	220	3	nakagata	nakagata	PROPN
aiua-14101	220	4	n	n	CCONJ
aiua-14101	220	5	,	,	PUNCT
aiua-14101	220	6	mikoda	mikoda	NOUN
aiua-14101	220	7	n	n	CCONJ
aiua-14101	220	8	,	,	PUNCT
aiua-14101	220	9	nakao	nakao	PROPN
aiua-14101	220	10	s	s	PROPN
aiua-14101	220	11	,	,	PUNCT
aiua-14101	220	12	et	et	PROPN
aiua-14101	220	13	al	al	PROPN
aiua-14101	220	14	.	.	PUNCT
aiua-14101	220	15	establishment	establishment	NOUN
aiua-14101	220	16	of	of	ADP
aiua-14101	220	17	sperm	sperm	NOUN
aiua-14101	220	18	cryopreservation	cryopreservation	NOUN
aiua-14101	220	19	and	and	CCONJ
aiua-14101	220	20	in	in	ADP
aiua-14101	220	21	vitro	vitro	X
aiua-14101	220	22	fertilisation	fertilisation	NOUN
aiua-14101	220	23	protocols	protocol	NOUN
aiua-14101	220	24	for	for	ADP
aiua-14101	220	25	rats	rat	NOUN
aiua-14101	220	26	.	.	PUNCT
aiua-14101	221	1	sci	sci	PROPN
aiua-14101	221	2	rep	rep	PROPN
aiua-14101	221	3	.	.	PROPN
aiua-14101	221	4	2020	2020	NUM
aiua-14101	221	5	;	;	PUNCT
aiua-14101	221	6	10:1026	10:1026	NUM
aiua-14101	221	7	.	.	NOUN
aiua-14101	222	1	4	4	NUM
aiua-14101	222	2	.	.	X
aiua-14101	222	3	kim	kim	PROPN
aiua-14101	222	4	s	s	PROPN
aiua-14101	222	5	,	,	PUNCT
aiua-14101	222	6	agca	agca	PROPN
aiua-14101	222	7	c	c	NOUN
aiua-14101	222	8	,	,	PUNCT
aiua-14101	222	9	agca	agca	PROPN
aiua-14101	222	10	y.	y.	NOUN
aiua-14101	222	11	changes	change	NOUN
aiua-14101	222	12	in	in	ADP
aiua-14101	222	13	rat	rat	NOUN
aiua-14101	222	14	spermatozoa	spermatozoa	NOUN
aiua-14101	222	15	function	function	NOUN
aiua-14101	222	16	after	after	ADP
aiua-14101	222	17	cooling	cool	VERB
aiua-14101	222	18	,	,	PUNCT
aiua-14101	222	19	cryopreservation	cryopreservation	NOUN
aiua-14101	222	20	and	and	CCONJ
aiua-14101	222	21	centrifugation	centrifugation	NOUN
aiua-14101	222	22	processes	process	NOUN
aiua-14101	222	23	.	.	PUNCT
aiua-14101	223	1	cryobiology	cryobiology	NOUN
aiua-14101	223	2	.	.	PUNCT
aiua-14101	224	1	2012	2012	NUM
aiua-14101	224	2	;	;	PUNCT
aiua-14101	224	3	65:215	65:215	NUM
aiua-14101	224	4	-	-	SYM
aiua-14101	224	5	23	23	NUM
aiua-14101	224	6	.	.	PUNCT
aiua-14101	225	1	5	5	X
aiua-14101	225	2	.	.	X
aiua-14101	225	3	nakata	nakata	PROPN
aiua-14101	225	4	m	m	PROPN
aiua-14101	225	5	,	,	PUNCT
aiua-14101	225	6	okuda	okuda	VERB
aiua-14101	225	7	y	y	PROPN
aiua-14101	225	8	,	,	PUNCT
aiua-14101	225	9	yamashita	yamashita	PROPN
aiua-14101	225	10	y	y	PROPN
aiua-14101	225	11	,	,	PUNCT
aiua-14101	225	12	et	et	PROPN
aiua-14101	225	13	al	al	PROPN
aiua-14101	225	14	.	.	PROPN
aiua-14101	226	1	successful	successful	ADJ
aiua-14101	226	2	production	production	NOUN
aiua-14101	226	3	of	of	ADP
aiua-14101	226	4	offspring	offspring	NOUN
aiua-14101	226	5	using	use	VERB
aiua-14101	226	6	cryopreserved	cryopreserved	ADJ
aiua-14101	226	7	sperm	sperm	NOUN
aiua-14101	226	8	via	via	ADP
aiua-14101	226	9	nonsurgical	nonsurgical	ADJ
aiua-14101	226	10	artificial	artificial	ADJ
aiua-14101	226	11	insemination	insemination	NOUN
aiua-14101	226	12	in	in	ADP
aiua-14101	226	13	rats	rat	NOUN
aiua-14101	226	14	.	.	PUNCT
aiua-14101	227	1	j	j	PROPN
aiua-14101	227	2	reprod	reprod	PROPN
aiua-14101	227	3	dev	dev	PROPN
aiua-14101	227	4	.	.	PROPN
aiua-14101	227	5	2012	2012	NUM
aiua-14101	227	6	;	;	PUNCT
aiua-14101	227	7	58:501	58:501	NUM
aiua-14101	227	8	-	-	SYM
aiua-14101	227	9	4	4	NUM
aiua-14101	227	10	.	.	NOUN
aiua-14101	227	11	6	6	NUM
aiua-14101	227	12	.	.	X
aiua-14101	227	13	nakatsukasa	nakatsukasa	NOUN
aiua-14101	228	1	e	e	NOUN
aiua-14101	228	2	,	,	PUNCT
aiua-14101	228	3	inomata	inomata	PROPN
aiua-14101	228	4	t	t	PROPN
aiua-14101	228	5	,	,	PUNCT
aiua-14101	228	6	ikeda	ikeda	PROPN
aiua-14101	228	7	t	t	PROPN
aiua-14101	228	8	,	,	PUNCT
aiua-14101	228	9	et	et	PROPN
aiua-14101	228	10	al	al	PROPN
aiua-14101	228	11	.	.	PUNCT
aiua-14101	228	12	generation	generation	NOUN
aiua-14101	228	13	of	of	ADP
aiua-14101	228	14	live	live	ADJ
aiua-14101	228	15	rat	rat	NOUN
aiua-14101	228	16	offspring	offspring	NOUN
aiua-14101	228	17	by	by	ADP
aiua-14101	228	18	intrauterine	intrauterine	ADJ
aiua-14101	228	19	insemination	insemination	NOUN
aiua-14101	228	20	with	with	ADP
aiua-14101	228	21	epididymal	epididymal	ADJ
aiua-14101	228	22	spermatozoa	spermatozoa	NOUN
aiua-14101	228	23	cryopreserved	cryopreserve	VERB
aiua-14101	228	24	at	at	ADP
aiua-14101	228	25	-196	-196	PROPN
aiua-14101	228	26	°	°	PROPN
aiua-14101	228	27	c	c	NOUN
aiua-14101	228	28	.	.	PUNCT
aiua-14101	229	1	reproduction	reproduction	NOUN
aiua-14101	229	2	.	.	PUNCT
aiua-14101	230	1	2001	2001	NUM
aiua-14101	230	2	;	;	PUNCT
aiua-14101	230	3	122:463	122:463	X
aiua-14101	230	4	-	-	SYM
aiua-14101	230	5	7	7	NUM
aiua-14101	230	6	.	.	NOUN
aiua-14101	230	7	7	7	NUM
aiua-14101	230	8	.	.	X
aiua-14101	231	1	kutluyer	kutluyer	PROPN
aiua-14101	231	2	f	f	PROPN
aiua-14101	231	3	,	,	PUNCT
aiua-14101	231	4	aksu	aksu	PROPN
aiua-14101	231	5	ö	ö	PROPN
aiua-14101	231	6	,	,	PUNCT
aiua-14101	231	7	kocabas	kocabas	PROPN
aiua-14101	231	8	m.	m.	PROPN
aiua-14101	231	9	effect	effect	NOUN
aiua-14101	231	10	of	of	ADP
aiua-14101	231	11	l	l	NOUN
aiua-14101	231	12	-	-	NOUN
aiua-14101	231	13	tryptophan	tryptophan	NOUN
aiua-14101	231	14	on	on	ADP
aiua-14101	231	15	sperm	sperm	NOUN
aiua-14101	231	16	quality	quality	NOUN
aiua-14101	231	17	of	of	ADP
aiua-14101	231	18	tigris	tigris	NOUN
aiua-14101	231	19	scraper	scraper	NOUN
aiua-14101	231	20	(	(	PUNCT
aiua-14101	231	21	capoeta	capoeta	ADJ
aiua-14101	231	22	umbla	umbla	NOUN
aiua-14101	231	23	)	)	PUNCT
aiua-14101	231	24	(	(	PUNCT
aiua-14101	231	25	pisces	pisce	NOUN
aiua-14101	231	26	:	:	PUNCT
aiua-14101	231	27	cyprinidae	cyprinidae	NOUN
aiua-14101	231	28	)	)	PUNCT
aiua-14101	231	29	after	after	ADP
aiua-14101	231	30	cryopreservation	cryopreservation	NOUN
aiua-14101	231	31	.	.	PUNCT
aiua-14101	232	1	cryo	cryo	PROPN
aiua-14101	232	2	letters	letter	NOUN
aiua-14101	232	3	.	.	PUNCT
aiua-14101	233	1	2019	2019	NUM
aiua-14101	233	2	;	;	PUNCT
aiua-14101	233	3	40:77	40:77	NUM
aiua-14101	233	4	-	-	SYM
aiua-14101	233	5	82	82	NUM
aiua-14101	233	6	.	.	NOUN
aiua-14101	233	7	8	8	NUM
aiua-14101	233	8	.	.	NOUN
aiua-14101	233	9	lahnsteiner	lahnsteiner	PROPN
aiua-14101	234	1	f	f	PROPN
aiua-14101	234	2	,	,	PUNCT
aiua-14101	234	3	berger	berger	PROPN
aiua-14101	234	4	b	b	PROPN
aiua-14101	234	5	,	,	PUNCT
aiua-14101	234	6	weismann	weismann	PROPN
aiua-14101	234	7	t	t	PROPN
aiua-14101	234	8	,	,	PUNCT
aiua-14101	234	9	et	et	PROPN
aiua-14101	234	10	al	al	PROPN
aiua-14101	234	11	.	.	PUNCT
aiua-14101	235	1	the	the	DET
aiua-14101	235	2	influence	influence	NOUN
aiua-14101	235	3	of	of	ADP
aiua-14101	235	4	various	various	ADJ
aiua-14101	235	5	cryoprotectants	cryoprotectant	NOUN
aiua-14101	235	6	on	on	ADP
aiua-14101	235	7	semen	semen	NOUN
aiua-14101	235	8	quality	quality	NOUN
aiua-14101	235	9	of	of	ADP
aiua-14101	235	10	the	the	DET
aiua-14101	235	11	rainbow	rainbow	NOUN
aiua-14101	235	12	trout	trout	NOUN
aiua-14101	235	13	(	(	PUNCT
aiua-14101	235	14	oncorhynchus	oncorhynchus	NOUN
aiua-14101	235	15	mykiss	mykiss	NOUN
aiua-14101	235	16	)	)	PUNCT
aiua-14101	235	17	before	before	ADP
aiua-14101	235	18	and	and	CCONJ
aiua-14101	235	19	after	after	ADP
aiua-14101	235	20	cryopreservation	cryopreservation	NOUN
aiua-14101	235	21	.	.	PUNCT
aiua-14101	236	1	j	j	PROPN
aiua-14101	236	2	appl	appl	PROPN
aiua-14101	236	3	ichthyol	ichthyol	PROPN
aiua-14101	236	4	.	.	PUNCT
aiua-14101	237	1	1996	1996	NUM
aiua-14101	237	2	;	;	PUNCT
aiua-14101	237	3	12:99	12:99	NUM
aiua-14101	237	4	-	-	SYM
aiua-14101	237	5	106	106	NUM
aiua-14101	237	6	.	.	PUNCT
aiua-14101	238	1	9	9	NUM
aiua-14101	238	2	.	.	X
aiua-14101	238	3	nayak	nayak	PROPN
aiua-14101	238	4	bn	bn	PROPN
aiua-14101	238	5	,	,	PUNCT
aiua-14101	238	6	buttar	buttar	PROPN
aiua-14101	238	7	hs	hs	PROPN
aiua-14101	238	8	.	.	PROPN
aiua-14101	238	9	evaluation	evaluation	NOUN
aiua-14101	238	10	of	of	ADP
aiua-14101	238	11	the	the	DET
aiua-14101	238	12	antioxidant	antioxidant	ADJ
aiua-14101	238	13	properties	property	NOUN
aiua-14101	238	14	of	of	ADP
aiua-14101	238	15	tryptophan	tryptophan	NOUN
aiua-14101	238	16	and	and	CCONJ
aiua-14101	238	17	its	its	PRON
aiua-14101	238	18	metabolites	metabolite	NOUN
aiua-14101	238	19	in	in	ADP
aiua-14101	238	20	in	in	ADP
aiua-14101	238	21	vitro	vitro	X
aiua-14101	238	22	assay	assay	PROPN
aiua-14101	238	23	.	.	PUNCT
aiua-14101	239	1	j	j	PROPN
aiua-14101	239	2	complement	complement	PROPN
aiua-14101	239	3	integr	integr	PROPN
aiua-14101	239	4	med	me	VERB
aiua-14101	239	5	.	.	PROPN
aiua-14101	240	1	2016	2016	NUM
aiua-14101	240	2	;	;	PUNCT
aiua-14101	240	3	13:129	13:129	NUM
aiua-14101	240	4	-	-	SYM
aiua-14101	240	5	36	36	NUM
aiua-14101	240	6	.	.	PUNCT
aiua-14101	241	1	10	10	NUM
aiua-14101	241	2	.	.	PUNCT
aiua-14101	241	3	xu	xu	PROPN
aiua-14101	242	1	k	k	PROPN
aiua-14101	242	2	,	,	PUNCT
aiua-14101	242	3	liu	liu	PROPN
aiua-14101	242	4	g	g	PROPN
aiua-14101	242	5	,	,	PUNCT
aiua-14101	242	6	fu	fu	PROPN
aiua-14101	242	7	c.	c.	PROPN
aiua-14101	242	8	the	the	DET
aiua-14101	242	9	tryptophan	tryptophan	NOUN
aiua-14101	242	10	pathway	pathway	NOUN
aiua-14101	242	11	targeting	target	VERB
aiua-14101	242	12	antioxidant	antioxidant	ADJ
aiua-14101	242	13	capacity	capacity	NOUN
aiua-14101	242	14	in	in	ADP
aiua-14101	242	15	the	the	DET
aiua-14101	242	16	placenta	placenta	NOUN
aiua-14101	242	17	.	.	PUNCT
aiua-14101	243	1	oxid	oxid	PROPN
aiua-14101	243	2	med	med	PROPN
aiua-14101	243	3	cell	cell	NOUN
aiua-14101	243	4	longev	longev	NOUN
aiua-14101	243	5	.	.	PUNCT
aiua-14101	243	6	2018	2018	NUM
aiua-14101	243	7	;	;	PUNCT
aiua-14101	243	8	2018:1054797	2018:1054797	NUM
aiua-14101	243	9	.	.	NOUN
aiua-14101	243	10	11	11	NUM
aiua-14101	243	11	.	.	PUNCT
aiua-14101	244	1	richard	richard	PROPN
aiua-14101	244	2	dm	dm	PROPN
aiua-14101	244	3	,	,	PUNCT
aiua-14101	244	4	dawes	dawes	PROPN
aiua-14101	244	5	ma	ma	PROPN
aiua-14101	244	6	,	,	PUNCT
aiua-14101	244	7	mathias	mathias	PROPN
aiua-14101	244	8	cw	cw	PROPN
aiua-14101	244	9	,	,	PUNCT
aiua-14101	244	10	et	et	PROPN
aiua-14101	244	11	al	al	PROPN
aiua-14101	244	12	.	.	PUNCT
aiua-14101	245	1	l	l	NOUN
aiua-14101	245	2	-	-	NOUN
aiua-14101	245	3	tryptophan	tryptophan	NOUN
aiua-14101	245	4	:	:	PUNCT
aiua-14101	245	5	basic	basic	ADJ
aiua-14101	245	6	metabolic	metabolic	NOUN
aiua-14101	245	7	functions	function	NOUN
aiua-14101	245	8	,	,	PUNCT
aiua-14101	245	9	behavioral	behavioral	ADJ
aiua-14101	245	10	research	research	NOUN
aiua-14101	245	11	and	and	CCONJ
aiua-14101	245	12	therapeutic	therapeutic	ADJ
aiua-14101	245	13	indications	indication	NOUN
aiua-14101	245	14	.	.	PUNCT
aiua-14101	246	1	int	int	NOUN
aiua-14101	246	2	j	j	PROPN
aiua-14101	246	3	tryptophan	tryptophan	NOUN
aiua-14101	246	4	res	re	NOUN
aiua-14101	246	5	.	.	PUNCT
aiua-14101	246	6	2009	2009	NUM
aiua-14101	246	7	;	;	PUNCT
aiua-14101	246	8	2:45	2:45	NUM
aiua-14101	246	9	-	-	SYM
aiua-14101	246	10	60	60	NUM
aiua-14101	246	11	.	.	PUNCT
aiua-14101	247	1	12	12	NUM
aiua-14101	247	2	.	.	PUNCT
aiua-14101	247	3	fuller	full	ADJ
aiua-14101	247	4	bj	bj	NOUN
aiua-14101	247	5	.	.	PUNCT
aiua-14101	248	1	cryoprotectants	cryoprotectant	NOUN
aiua-14101	248	2	:	:	PUNCT
aiua-14101	248	3	the	the	DET
aiua-14101	248	4	essential	essential	ADJ
aiua-14101	248	5	antifreezes	antifreeze	VERB
aiua-14101	248	6	to	to	PART
aiua-14101	248	7	protect	protect	VERB
aiua-14101	248	8	life	life	NOUN
aiua-14101	248	9	in	in	ADP
aiua-14101	248	10	the	the	DET
aiua-14101	248	11	frozen	frozen	ADJ
aiua-14101	248	12	state	state	NOUN
aiua-14101	248	13	.	.	PUNCT
aiua-14101	249	1	cryo	cryo	NOUN
aiua-14101	249	2	letters	letter	NOUN
aiua-14101	249	3	.	.	PUNCT
aiua-14101	250	1	2004	2004	NUM
aiua-14101	250	2	;	;	PUNCT
aiua-14101	250	3	25:375	25:375	NUM
aiua-14101	250	4	-	-	SYM
aiua-14101	250	5	88	88	NUM
aiua-14101	250	6	.	.	PUNCT
aiua-14101	251	1	13	13	NUM
aiua-14101	251	2	.	.	X
aiua-14101	251	3	aboagla	aboagla	PROPN
aiua-14101	251	4	eme	eme	PROPN
aiua-14101	251	5	,	,	PUNCT
aiua-14101	251	6	terada	terada	PROPN
aiua-14101	251	7	t.	t.	NOUN
aiua-14101	251	8	effects	effect	NOUN
aiua-14101	251	9	of	of	ADP
aiua-14101	251	10	the	the	DET
aiua-14101	251	11	supplementation	supplementation	NOUN
aiua-14101	251	12	of	of	ADP
aiua-14101	251	13	trehalose	trehalose	PRON
aiua-14101	251	14	extender	extender	NOUN
aiua-14101	251	15	containing	contain	VERB
aiua-14101	251	16	egg	egg	NOUN
aiua-14101	251	17	yolk	yolk	NOUN
aiua-14101	251	18	with	with	ADP
aiua-14101	251	19	sodium	sodium	NOUN
aiua-14101	251	20	dodecyl	dodecyl	NOUN
aiua-14101	251	21	sulfate	sulfate	VERB
aiua-14101	251	22	on	on	ADP
aiua-14101	251	23	the	the	DET
aiua-14101	251	24	freezability	freezability	NOUN
aiua-14101	251	25	of	of	ADP
aiua-14101	251	26	goat	goat	PROPN
aiua-14101	251	27	spermatozoa	spermatozoa	PROPN
aiua-14101	251	28	.	.	PUNCT
aiua-14101	252	1	theriogenology	theriogenology	PROPN
aiua-14101	252	2	.	.	PUNCT
aiua-14101	253	1	2004	2004	NUM
aiua-14101	253	2	;	;	PUNCT
aiua-14101	253	3	62:80918	62:80918	NUM
aiua-14101	253	4	.	.	PUNCT
aiua-14101	254	1	14	14	NUM
aiua-14101	254	2	.	.	PUNCT
aiua-14101	255	1	quinn	quinn	PROPN
aiua-14101	255	2	p	p	NOUN
aiua-14101	255	3	,	,	PUNCT
aiua-14101	255	4	kerin	kerin	PROPN
aiua-14101	255	5	jf	jf	PROPN
aiua-14101	255	6	,	,	PUNCT
aiua-14101	255	7	warnes	warnes	PROPN
aiua-14101	255	8	gm	gm	PROPN
aiua-14101	255	9	.	.	PUNCT
aiua-14101	256	1	improved	improve	VERB
aiua-14101	256	2	pregnancy	pregnancy	NOUN
aiua-14101	256	3	rate	rate	NOUN
aiua-14101	256	4	in	in	ADP
aiua-14101	256	5	human	human	NOUN
aiua-14101	256	6	in	in	ADP
aiua-14101	256	7	vitro	vitro	X
aiua-14101	256	8	fertilization	fertilization	NOUN
aiua-14101	256	9	with	with	ADP
aiua-14101	256	10	the	the	DET
aiua-14101	256	11	use	use	NOUN
aiua-14101	256	12	of	of	ADP
aiua-14101	256	13	a	a	DET
aiua-14101	256	14	medium	medium	NOUN
aiua-14101	256	15	based	base	VERB
aiua-14101	256	16	on	on	ADP
aiua-14101	256	17	the	the	DET
aiua-14101	256	18	composition	composition	NOUN
aiua-14101	256	19	of	of	ADP
aiua-14101	256	20	human	human	ADJ
aiua-14101	256	21	tubal	tubal	ADJ
aiua-14101	256	22	fluid	fluid	NOUN
aiua-14101	256	23	.	.	PUNCT
aiua-14101	257	1	fertil	fertil	PROPN
aiua-14101	257	2	steril	steril	PROPN
aiua-14101	257	3	.	.	PROPN
aiua-14101	257	4	1985	1985	NUM
aiua-14101	257	5	;	;	PUNCT
aiua-14101	257	6	44:493	44:493	NUM
aiua-14101	257	7	-	-	SYM
aiua-14101	257	8	8	8	NUM
aiua-14101	257	9	.	.	NOUN
aiua-14101	257	10	15	15	NUM
aiua-14101	257	11	.	.	PUNCT
aiua-14101	258	1	world	world	PROPN
aiua-14101	258	2	health	health	PROPN
aiua-14101	258	3	organization	organization	PROPN
aiua-14101	258	4	.	.	PUNCT
aiua-14101	259	1	who	who	PRON
aiua-14101	259	2	laboratory	laboratory	VERB
aiua-14101	259	3	manual	manual	NOUN
aiua-14101	259	4	for	for	ADP
aiua-14101	259	5	the	the	DET
aiua-14101	259	6	examination	examination	NOUN
aiua-14101	259	7	of	of	ADP
aiua-14101	259	8	human	human	ADJ
aiua-14101	259	9	semen	semen	NOUN
aiua-14101	259	10	and	and	CCONJ
aiua-14101	259	11	sperm	sperm	NOUN
aiua-14101	259	12	-	-	PUNCT
aiua-14101	259	13	cervical	cervical	ADJ
aiua-14101	259	14	mucus	mucus	NOUN
aiua-14101	259	15	interaction	interaction	NOUN
aiua-14101	259	16	.	.	PUNCT
aiua-14101	260	1	cambridge	cambridge	PROPN
aiua-14101	260	2	:	:	PUNCT
aiua-14101	260	3	cambridge	cambridge	PROPN
aiua-14101	260	4	university	university	PROPN
aiua-14101	260	5	press	press	NOUN
aiua-14101	260	6	;	;	PUNCT
aiua-14101	260	7	1999	1999	NUM
aiua-14101	260	8	.	.	PUNCT
aiua-14101	261	1	16	16	NUM
aiua-14101	261	2	.	.	PUNCT
aiua-14101	262	1	agarwal	agarwal	PROPN
aiua-14101	262	2	a	a	PROPN
aiua-14101	262	3	,	,	PUNCT
aiua-14101	262	4	gupta	gupta	PROPN
aiua-14101	262	5	s	s	PART
aiua-14101	262	6	,	,	PUNCT
aiua-14101	262	7	sharma	sharma	PROPN
aiua-14101	262	8	r.	r.	PROPN
aiua-14101	262	9	eosin	eosin	PROPN
aiua-14101	262	10	-	-	PUNCT
aiua-14101	262	11	nigrosin	nigrosin	NOUN
aiua-14101	262	12	staining	stain	VERB
aiua-14101	262	13	procedure	procedure	NOUN
aiua-14101	262	14	.	.	PUNCT
aiua-14101	263	1	in	in	ADP
aiua-14101	263	2	:	:	PUNCT
aiua-14101	263	3	agarwal	agarwal	PROPN
aiua-14101	263	4	a	a	X
aiua-14101	263	5	,	,	PUNCT
aiua-14101	263	6	gupta	gupta	PROPN
aiua-14101	263	7	s	s	PART
aiua-14101	263	8	,	,	PUNCT
aiua-14101	263	9	sharma	sharma	PROPN
aiua-14101	263	10	r	r	PROPN
aiua-14101	263	11	,	,	PUNCT
aiua-14101	263	12	eds	ed	NOUN
aiua-14101	263	13	.	.	PUNCT
aiua-14101	263	14	andrological	andrological	ADJ
aiua-14101	263	15	evaluation	evaluation	NOUN
aiua-14101	263	16	of	of	ADP
aiua-14101	263	17	male	male	ADJ
aiua-14101	263	18	infertility	infertility	NOUN
aiua-14101	263	19	.	.	PUNCT
aiua-14101	264	1	cham	cham	PROPN
aiua-14101	264	2	:	:	PUNCT
aiua-14101	264	3	springer	springer	NOUN
aiua-14101	264	4	;	;	PUNCT
aiua-14101	264	5	2016	2016	NUM
aiua-14101	264	6	.	.	PUNCT
aiua-14101	265	1	pp	pp	ADV
aiua-14101	265	2	73	73	NUM
aiua-14101	265	3	-	-	SYM
aiua-14101	265	4	7	7	NUM
aiua-14101	265	5	.	.	NUM
aiua-14101	265	6	17	17	NUM
aiua-14101	265	7	.	.	PUNCT
aiua-14101	266	1	jeyendran	jeyendran	PROPN
aiua-14101	266	2	rs	rs	PROPN
aiua-14101	266	3	,	,	PUNCT
aiua-14101	266	4	van	van	PROPN
aiua-14101	266	5	der	der	NOUN
aiua-14101	266	6	ven	ven	PROPN
aiua-14101	266	7	hh	hh	PROPN
aiua-14101	266	8	,	,	PUNCT
aiua-14101	266	9	perez	perez	PROPN
aiua-14101	266	10	-	-	PUNCT
aiua-14101	266	11	pelaez	pelaez	PROPN
aiua-14101	266	12	m	m	PROPN
aiua-14101	266	13	,	,	PUNCT
aiua-14101	266	14	et	et	PROPN
aiua-14101	266	15	al	al	PROPN
aiua-14101	266	16	.	.	PUNCT
aiua-14101	267	1	development	development	NOUN
aiua-14101	267	2	of	of	ADP
aiua-14101	267	3	an	an	DET
aiua-14101	267	4	assay	assay	NOUN
aiua-14101	267	5	to	to	PART
aiua-14101	267	6	assess	assess	VERB
aiua-14101	267	7	the	the	DET
aiua-14101	267	8	functional	functional	ADJ
aiua-14101	267	9	integrity	integrity	NOUN
aiua-14101	267	10	of	of	ADP
aiua-14101	267	11	the	the	DET
aiua-14101	267	12	human	human	ADJ
aiua-14101	267	13	sperm	sperm	NOUN
aiua-14101	267	14	membrane	membrane	NOUN
aiua-14101	267	15	and	and	CCONJ
aiua-14101	267	16	its	its	PRON
aiua-14101	267	17	relationship	relationship	NOUN
aiua-14101	267	18	to	to	ADP
aiua-14101	267	19	other	other	ADJ
aiua-14101	267	20	semen	semen	NOUN
aiua-14101	267	21	characteristics	characteristic	NOUN
aiua-14101	267	22	.	.	PUNCT
aiua-14101	268	1	j	j	PROPN
aiua-14101	268	2	reprod	reprod	PROPN
aiua-14101	268	3	fertil	fertil	PROPN
aiua-14101	268	4	.	.	PUNCT
aiua-14101	268	5	1984	1984	NUM
aiua-14101	268	6	;	;	PUNCT
aiua-14101	268	7	70:219	70:219	NUM
aiua-14101	268	8	-	-	SYM
aiua-14101	268	9	28	28	NUM
aiua-14101	268	10	.	.	PUNCT
aiua-14101	269	1	18	18	NUM
aiua-14101	269	2	.	.	PUNCT
aiua-14101	269	3	somfai	somfai	PROPN
aiua-14101	269	4	t	t	PROPN
aiua-14101	269	5	,	,	PUNCT
aiua-14101	269	6	bodó	bodó	PROPN
aiua-14101	269	7	s	s	PROPN
aiua-14101	269	8	,	,	PUNCT
aiua-14101	269	9	nagy	nagy	PROPN
aiua-14101	269	10	s	s	PROPN
aiua-14101	269	11	,	,	PUNCT
aiua-14101	269	12	et	et	PROPN
aiua-14101	269	13	al	al	PROPN
aiua-14101	269	14	.	.	PROPN
aiua-14101	269	15	effect	effect	NOUN
aiua-14101	269	16	of	of	ADP
aiua-14101	269	17	swim	swim	VERB
aiua-14101	269	18	up	up	ADP
aiua-14101	269	19	and	and	CCONJ
aiua-14101	269	20	percoll	percoll	NOUN
aiua-14101	269	21	treatment	treatment	NOUN
aiua-14101	269	22	on	on	ADP
aiua-14101	269	23	viability	viability	NOUN
aiua-14101	269	24	and	and	CCONJ
aiua-14101	269	25	acrosome	acrosome	NOUN
aiua-14101	269	26	integrity	integrity	NOUN
aiua-14101	269	27	of	of	ADP
aiua-14101	269	28	frozen	frozen	ADJ
aiua-14101	269	29	-	-	PUNCT
aiua-14101	269	30	thawed	thaw	VERB
aiua-14101	269	31	bull	bull	NOUN
aiua-14101	269	32	spermatozoa	spermatozoon	NOUN
aiua-14101	269	33	.	.	PUNCT
aiua-14101	270	1	reprod	reprod	PROPN
aiua-14101	270	2	domest	domest	PROPN
aiua-14101	270	3	anim	anim	PROPN
aiua-14101	270	4	.	.	PUNCT
aiua-14101	271	1	2002	2002	NUM
aiua-14101	271	2	;	;	PUNCT
aiua-14101	271	3	37:285	37:285	NUM
aiua-14101	271	4	-	-	SYM
aiua-14101	271	5	90	90	NUM
aiua-14101	271	6	.	.	PUNCT
aiua-14101	272	1	declarations	declaration	NOUN
aiua-14101	272	2	ethical	ethical	ADJ
aiua-14101	272	3	approval	approval	NOUN
aiua-14101	272	4	:	:	PUNCT
aiua-14101	272	5	ethical	ethical	ADJ
aiua-14101	272	6	approval	approval	NOUN
aiua-14101	272	7	for	for	ADP
aiua-14101	272	8	this	this	DET
aiua-14101	272	9	study	study	NOUN
aiua-14101	272	10	was	be	AUX
aiua-14101	272	11	obtained	obtain	VERB
aiua-14101	272	12	by	by	ADP
aiua-14101	272	13	the	the	DET
aiua-14101	272	14	animal	animal	NOUN
aiua-14101	272	15	experiments	experiment	VERB
aiua-14101	272	16	local	local	ADJ
aiua-14101	272	17	ethics	ethic	NOUN
aiua-14101	272	18	committee	committee	PROPN
aiua-14101	272	19	(	(	PUNCT
aiua-14101	272	20	hadyek	hadyek	NOUN
aiua-14101	272	21	)	)	PUNCT
aiua-14101	272	22	of	of	ADP
aiua-14101	272	23	hatay	hatay	NOUN
aiua-14101	272	24	mustafa	mustafa	PROPN
aiua-14101	272	25	kemal	kemal	PROPN
aiua-14101	272	26	university	university	PROPN
aiua-14101	272	27	,	,	PUNCT
aiua-14101	272	28	hatay	hatay	NOUN
aiua-14101	272	29	,	,	PUNCT
aiua-14101	272	30	türkiye	türkiye	PROPN
aiua-14101	272	31	(	(	PUNCT
aiua-14101	272	32	ethical	ethical	ADJ
aiua-14101	272	33	approval	approval	NOUN
aiua-14101	272	34	numbered	number	VERB
aiua-14101	272	35	2024/09	2024/09	NUM
aiua-14101	272	36	-	-	SYM
aiua-14101	272	37	01	01	NUM
aiua-14101	272	38	)	)	PUNCT
aiua-14101	272	39	.	.	PUNCT
aiua-14101	273	1	availability	availability	NOUN
aiua-14101	273	2	of	of	ADP
aiua-14101	273	3	data	datum	NOUN
aiua-14101	273	4	and	and	CCONJ
aiua-14101	273	5	material	material	NOUN
aiua-14101	273	6	:	:	PUNCT
aiua-14101	273	7	the	the	DET
aiua-14101	273	8	data	datum	NOUN
aiua-14101	273	9	used	use	VERB
aiua-14101	273	10	in	in	ADP
aiua-14101	273	11	this	this	DET
aiua-14101	273	12	study	study	NOUN
aiua-14101	273	13	were	be	AUX
aiua-14101	273	14	generated	generate	VERB
aiua-14101	273	15	by	by	ADP
aiua-14101	273	16	the	the	DET
aiua-14101	273	17	author(s	author(s	NOUN
aiua-14101	273	18	)	)	PUNCT
aiua-14101	273	19	and	and	CCONJ
aiua-14101	273	20	are	be	AUX
aiua-14101	273	21	available	available	ADJ
aiua-14101	273	22	from	from	ADP
aiua-14101	273	23	the	the	DET
aiua-14101	273	24	corresponding	corresponding	ADJ
aiua-14101	273	25	author	author	NOUN
aiua-14101	273	26	upon	upon	SCONJ
aiua-14101	273	27	reasonable	reasonable	ADJ
aiua-14101	273	28	request	request	NOUN
aiua-14101	273	29	.	.	PUNCT
aiua-14101	274	1	competing	compete	VERB
aiua-14101	274	2	interests	interest	NOUN
aiua-14101	274	3	:	:	PUNCT
aiua-14101	274	4	none	none	NOUN
aiua-14101	274	5	of	of	ADP
aiua-14101	274	6	the	the	DET
aiua-14101	274	7	authors	author	NOUN
aiua-14101	274	8	have	have	VERB
aiua-14101	274	9	any	any	DET
aiua-14101	274	10	conflicts	conflict	NOUN
aiua-14101	274	11	of	of	ADP
aiua-14101	274	12	interest	interest	NOUN
aiua-14101	274	13	to	to	PART
aiua-14101	274	14	declare	declare	VERB
aiua-14101	274	15	that	that	PRON
aiua-14101	274	16	are	be	AUX
aiua-14101	274	17	either	either	CCONJ
aiua-14101	274	18	directly	directly	ADV
aiua-14101	274	19	or	or	CCONJ
aiua-14101	274	20	indirectly	indirectly	ADV
aiua-14101	274	21	related	relate	VERB
aiua-14101	274	22	to	to	ADP
aiua-14101	274	23	the	the	DET
aiua-14101	274	24	study	study	NOUN
aiua-14101	274	25	design	design	NOUN
aiua-14101	274	26	,	,	PUNCT
aiua-14101	274	27	execution	execution	NOUN
aiua-14101	274	28	or	or	CCONJ
aiua-14101	274	29	reporting	reporting	NOUN
aiua-14101	274	30	of	of	ADP
aiua-14101	274	31	this	this	DET
aiua-14101	274	32	work	work	NOUN
aiua-14101	274	33	.	.	PUNCT
aiua-14101	275	1	funding	funding	NOUN
aiua-14101	275	2	:	:	PUNCT
aiua-14101	275	3	this	this	DET
aiua-14101	275	4	study	study	NOUN
aiua-14101	275	5	did	do	AUX
aiua-14101	275	6	not	not	PART
aiua-14101	275	7	receive	receive	VERB
aiua-14101	275	8	any	any	DET
aiua-14101	275	9	financial	financial	ADJ
aiua-14101	275	10	support	support	NOUN
aiua-14101	275	11	.	.	PUNCT
aiua-14101	276	1	authors	author	NOUN
aiua-14101	276	2	'	'	PART
aiua-14101	276	3	contributions	contribution	NOUN
aiua-14101	276	4	:	:	PUNCT
aiua-14101	276	5	f.y.d	f.y.d	PROPN
aiua-14101	276	6	.	.	PROPN
aiua-14101	276	7	,	,	PUNCT
aiua-14101	276	8	o.k.y	o.k.y	PROPN
aiua-14101	276	9	.	.	PUNCT
aiua-14101	277	1	and	and	CCONJ
aiua-14101	277	2	a.e.ş.	a.e.ş.	PROPN
aiua-14101	277	3	designed	design	VERB
aiua-14101	277	4	the	the	DET
aiua-14101	277	5	study	study	NOUN
aiua-14101	277	6	,	,	PUNCT
aiua-14101	277	7	performed	perform	VERB
aiua-14101	277	8	the	the	DET
aiua-14101	277	9	experiments	experiment	NOUN
aiua-14101	277	10	,	,	PUNCT
aiua-14101	277	11	performed	perform	VERB
aiua-14101	277	12	the	the	DET
aiua-14101	277	13	analyses	analysis	NOUN
aiua-14101	277	14	,	,	PUNCT
aiua-14101	277	15	and	and	CCONJ
aiua-14101	277	16	prepared	prepare	VERB
aiua-14101	277	17	the	the	DET
aiua-14101	277	18	first	first	ADJ
aiua-14101	277	19	drafts	draft	NOUN
aiua-14101	277	20	of	of	ADP
aiua-14101	277	21	the	the	DET
aiua-14101	277	22	manuscript	manuscript	NOUN
aiua-14101	277	23	based	base	VERB
aiua-14101	277	24	on	on	ADP
aiua-14101	277	25	the	the	DET
aiua-14101	277	26	data	datum	NOUN
aiua-14101	277	27	obtained	obtain	VERB
aiua-14101	277	28	.	.	PUNCT
aiua-14101	278	1	then	then	ADV
aiua-14101	278	2	,	,	PUNCT
aiua-14101	278	3	they	they	PRON
aiua-14101	278	4	made	make	VERB
aiua-14101	278	5	final	final	ADJ
aiua-14101	278	6	revisions	revision	NOUN
aiua-14101	278	7	on	on	ADP
aiua-14101	278	8	the	the	DET
aiua-14101	278	9	manuscript	manuscript	NOUN
aiua-14101	278	10	.	.	PUNCT
aiua-14101	279	1	all	all	DET
aiua-14101	279	2	authors	author	NOUN
aiua-14101	279	3	equally	equally	ADV
aiua-14101	279	4	contributed	contribute	VERB
aiua-14101	279	5	to	to	ADP
aiua-14101	279	6	the	the	DET
aiua-14101	279	7	interpretation	interpretation	NOUN
aiua-14101	279	8	of	of	ADP
aiua-14101	279	9	the	the	DET
aiua-14101	279	10	results	result	NOUN
aiua-14101	279	11	.	.	PUNCT
aiua-14101	280	1	acknowledgments	acknowledgment	NOUN
aiua-14101	280	2	:	:	PUNCT
aiua-14101	280	3	we	we	PRON
aiua-14101	280	4	would	would	AUX
aiua-14101	280	5	like	like	VERB
aiua-14101	280	6	to	to	PART
aiua-14101	280	7	express	express	VERB
aiua-14101	280	8	our	our	PRON
aiua-14101	280	9	gratitude	gratitude	NOUN
aiua-14101	280	10	to	to	ADP
aiua-14101	280	11	all	all	DET
aiua-14101	280	12	researchers	researcher	NOUN
aiua-14101	280	13	for	for	ADP
aiua-14101	280	14	their	their	PRON
aiua-14101	280	15	valuable	valuable	ADJ
aiua-14101	280	16	contributions	contribution	NOUN
aiua-14101	280	17	.	.	PUNCT
aiua-14101	281	1	archivio	archivio	NOUN
aiua-14101	281	2	italiano	italiano	PROPN
aiua-14101	281	3	di	di	PROPN
aiua-14101	281	4	urologia	urologia	PROPN
aiua-14101	281	5	e	e	PROPN
aiua-14101	281	6	andrologia	andrologia	NOUN
aiua-14101	281	7	2025	2025	NUM
aiua-14101	281	8	;	;	PUNCT
aiua-14101	281	9	97(3):14101	97(3):14101	NUM
aiua-14101	281	10	f.	f.	PROPN
aiua-14101	281	11	yilmaz	yilmaz	PROPN
aiua-14101	281	12	dayanc	dayanc	PROPN
aiua-14101	281	13	,	,	PUNCT
aiua-14101	281	14	o.	o.	PROPN
aiua-14101	281	15	kaan	kaan	PROPN
aiua-14101	281	16	yalcin	yalcin	PROPN
aiua-14101	281	17	,	,	PUNCT
aiua-14101	281	18	a.	a.	NOUN
aiua-14101	281	19	eraslan	eraslan	PROPN
aiua-14101	281	20	sakar	sakar	PROPN
aiua-14101	281	21	8	8	NUM
aiua-14101	281	22	19	19	NUM
aiua-14101	281	23	.	.	PUNCT
aiua-14101	282	1	hancock	hancock	PROPN
aiua-14101	282	2	jl	jl	PROPN
aiua-14101	282	3	.	.	PUNCT
aiua-14101	283	1	the	the	DET
aiua-14101	283	2	morphology	morphology	NOUN
aiua-14101	283	3	of	of	ADP
aiua-14101	283	4	bull	bull	NOUN
aiua-14101	283	5	spermatozoa	spermatozoon	NOUN
aiua-14101	283	6	.	.	PUNCT
aiua-14101	284	1	j	j	PROPN
aiua-14101	284	2	exp	exp	NOUN
aiua-14101	284	3	biol	biol	PROPN
aiua-14101	284	4	.	.	PUNCT
aiua-14101	285	1	1952	1952	NUM
aiua-14101	285	2	;	;	PUNCT
aiua-14101	285	3	29:445	29:445	NUM
aiua-14101	285	4	-	-	SYM
aiua-14101	285	5	53	53	NUM
aiua-14101	285	6	.	.	NOUN
aiua-14101	286	1	20	20	NUM
aiua-14101	286	2	.	.	PUNCT
aiua-14101	287	1	günay	günay	PROPN
aiua-14101	287	2	a	a	PRON
aiua-14101	287	3	,	,	PUNCT
aiua-14101	287	4	tanyolaç	tanyolaç	ADP
aiua-14101	287	5	a	a	DET
aiua-14101	287	6	,	,	PUNCT
aiua-14101	287	7	üstüner	üstüner	NOUN
aiua-14101	287	8	f.	f.	PROPN
aiua-14101	287	9	the	the	DET
aiua-14101	287	10	effects	effect	NOUN
aiua-14101	287	11	of	of	ADP
aiua-14101	287	12	different	different	ADJ
aiua-14101	287	13	staining	stain	VERB
aiua-14101	287	14	methods	method	NOUN
aiua-14101	287	15	on	on	ADP
aiua-14101	287	16	the	the	DET
aiua-14101	287	17	acrosome	acrosome	NOUN
aiua-14101	287	18	structure	structure	NOUN
aiua-14101	287	19	of	of	ADP
aiua-14101	287	20	ram	ram	NOUN
aiua-14101	287	21	spermatozoa	spermatozoon	NOUN
aiua-14101	287	22	.	.	PUNCT
aiua-14101	288	1	turk	turk	PROPN
aiua-14101	288	2	j	j	PROPN
aiua-14101	288	3	vet	vet	PROPN
aiua-14101	288	4	anim	anim	PROPN
aiua-14101	288	5	sci	sci	PROPN
aiua-14101	288	6	.	.	PROPN
aiua-14101	288	7	2003	2003	NUM
aiua-14101	288	8	;	;	PUNCT
aiua-14101	288	9	27:115	27:115	NUM
aiua-14101	288	10	-	-	SYM
aiua-14101	288	11	20	20	NUM
aiua-14101	288	12	.	.	PUNCT
aiua-14101	288	13	21	21	NUM
aiua-14101	288	14	.	.	PUNCT
aiua-14101	289	1	livak	livak	PROPN
aiua-14101	289	2	kj	kj	PROPN
aiua-14101	289	3	,	,	PUNCT
aiua-14101	289	4	schmittgen	schmittgen	PROPN
aiua-14101	289	5	td	td	NOUN
aiua-14101	289	6	.	.	PUNCT
aiua-14101	289	7	analysis	analysis	NOUN
aiua-14101	289	8	of	of	ADP
aiua-14101	289	9	relative	relative	ADJ
aiua-14101	289	10	gene	gene	NOUN
aiua-14101	289	11	expression	expression	NOUN
aiua-14101	289	12	data	datum	NOUN
aiua-14101	289	13	using	use	VERB
aiua-14101	289	14	real	real	ADJ
aiua-14101	289	15	-	-	PUNCT
aiua-14101	289	16	time	time	NOUN
aiua-14101	289	17	quantitative	quantitative	ADJ
aiua-14101	289	18	pcr	pcr	NOUN
aiua-14101	289	19	and	and	CCONJ
aiua-14101	289	20	the	the	DET
aiua-14101	289	21	2−δδct	2−δδct	NUM
aiua-14101	289	22	method	method	NOUN
aiua-14101	289	23	.	.	PUNCT
aiua-14101	290	1	methods	method	NOUN
aiua-14101	290	2	.	.	PUNCT
aiua-14101	291	1	2001	2001	NUM
aiua-14101	291	2	;	;	PUNCT
aiua-14101	291	3	25:402	25:402	NUM
aiua-14101	291	4	-	-	SYM
aiua-14101	291	5	8	8	NUM
aiua-14101	291	6	.	.	PUNCT
aiua-14101	292	1	22	22	NUM
aiua-14101	292	2	.	.	PUNCT
aiua-14101	293	1	seita	seita	PROPN
aiua-14101	293	2	y	y	PROPN
aiua-14101	293	3	,	,	PUNCT
aiua-14101	293	4	sugio	sugio	PROPN
aiua-14101	293	5	s	s	PROPN
aiua-14101	293	6	,	,	PUNCT
aiua-14101	293	7	ito	ito	PROPN
aiua-14101	293	8	j	j	PROPN
aiua-14101	293	9	,	,	PUNCT
aiua-14101	293	10	et	et	PROPN
aiua-14101	293	11	al	al	PROPN
aiua-14101	293	12	.	.	PUNCT
aiua-14101	293	13	generation	generation	NOUN
aiua-14101	293	14	of	of	ADP
aiua-14101	293	15	live	live	ADJ
aiua-14101	293	16	rats	rat	NOUN
aiua-14101	293	17	produced	produce	VERB
aiua-14101	293	18	by	by	ADP
aiua-14101	293	19	in	in	ADP
aiua-14101	293	20	vitro	vitro	X
aiua-14101	293	21	fertilization	fertilization	NOUN
aiua-14101	293	22	using	use	VERB
aiua-14101	293	23	cryopreserved	cryopreserved	ADJ
aiua-14101	293	24	spermatozoa	spermatozoon	NOUN
aiua-14101	293	25	.	.	PUNCT
aiua-14101	294	1	biol	biol	PROPN
aiua-14101	294	2	reprod	reprod	PROPN
aiua-14101	294	3	.	.	PUNCT
aiua-14101	294	4	2009	2009	NUM
aiua-14101	294	5	;	;	PUNCT
aiua-14101	294	6	80:503	80:503	NUM
aiua-14101	294	7	-	-	SYM
aiua-14101	294	8	10	10	NUM
aiua-14101	294	9	.	.	PUNCT
aiua-14101	295	1	23	23	NUM
aiua-14101	295	2	.	.	PUNCT
aiua-14101	296	1	agrawal	agrawal	PROPN
aiua-14101	296	2	p	p	X
aiua-14101	296	3	,	,	PUNCT
aiua-14101	296	4	magargee	magargee	PROPN
aiua-14101	296	5	sf	sf	PROPN
aiua-14101	296	6	,	,	PUNCT
aiua-14101	296	7	hammerstedt	hammerstedt	NOUN
aiua-14101	296	8	rh	rh	PROPN
aiua-14101	296	9	.	.	PROPN
aiua-14101	296	10	isolation	isolation	NOUN
aiua-14101	296	11	and	and	CCONJ
aiua-14101	296	12	characterization	characterization	NOUN
aiua-14101	296	13	of	of	ADP
aiua-14101	296	14	the	the	DET
aiua-14101	296	15	plasma	plasma	NOUN
aiua-14101	296	16	membrane	membrane	NOUN
aiua-14101	296	17	of	of	ADP
aiua-14101	296	18	rat	rat	NOUN
aiua-14101	296	19	cauda	cauda	NOUN
aiua-14101	296	20	epididymal	epididymal	ADJ
aiua-14101	296	21	spermatozoa	spermatozoa	NOUN
aiua-14101	296	22	.	.	PUNCT
aiua-14101	297	1	j	j	PROPN
aiua-14101	297	2	androl	androl	PROPN
aiua-14101	297	3	.	.	PROPN
aiua-14101	297	4	1988	1988	NUM
aiua-14101	297	5	;	;	PUNCT
aiua-14101	297	6	9:178	9:178	NUM
aiua-14101	297	7	-	-	SYM
aiua-14101	297	8	89	89	NUM
aiua-14101	297	9	.	.	PUNCT
aiua-14101	297	10	24	24	NUM
aiua-14101	297	11	.	.	PUNCT
aiua-14101	297	12	hammerstedt	hammerstedt	PROPN
aiua-14101	297	13	rh	rh	PROPN
aiua-14101	297	14	,	,	PUNCT
aiua-14101	297	15	graham	graham	PROPN
aiua-14101	297	16	jk	jk	PROPN
aiua-14101	297	17	,	,	PUNCT
aiua-14101	297	18	nolan	nolan	PROPN
aiua-14101	297	19	jp	jp	PROPN
aiua-14101	297	20	.	.	PUNCT
aiua-14101	298	1	cryopreservation	cryopreservation	NOUN
aiua-14101	298	2	of	of	ADP
aiua-14101	298	3	mammalian	mammalian	ADJ
aiua-14101	298	4	sperm	sperm	NOUN
aiua-14101	298	5	:	:	PUNCT
aiua-14101	298	6	what	what	PRON
aiua-14101	298	7	we	we	PRON
aiua-14101	298	8	ask	ask	VERB
aiua-14101	298	9	them	they	PRON
aiua-14101	298	10	to	to	PART
aiua-14101	298	11	survive	survive	VERB
aiua-14101	298	12	.	.	PUNCT
aiua-14101	299	1	j	j	PROPN
aiua-14101	299	2	androl	androl	PROPN
aiua-14101	299	3	.	.	PROPN
aiua-14101	299	4	1990	1990	NUM
aiua-14101	299	5	;	;	PUNCT
aiua-14101	299	6	11:73	11:73	NUM
aiua-14101	299	7	-	-	SYM
aiua-14101	299	8	88	88	NUM
aiua-14101	299	9	.	.	PUNCT
aiua-14101	300	1	25	25	NUM
aiua-14101	300	2	.	.	PUNCT
aiua-14101	301	1	tonieto	tonieto	PROPN
aiua-14101	301	2	ra	ra	PROPN
aiua-14101	301	3	,	,	PUNCT
aiua-14101	301	4	goularte	goularte	PROPN
aiua-14101	301	5	kl	kl	PROPN
aiua-14101	301	6	,	,	PUNCT
aiua-14101	301	7	gastal	gastal	PROPN
aiua-14101	301	8	gda	gda	PROPN
aiua-14101	301	9	,	,	PUNCT
aiua-14101	301	10	et	et	PROPN
aiua-14101	301	11	al	al	PROPN
aiua-14101	301	12	.	.	PROPN
aiua-14101	301	13	cryoprotectant	cryoprotectant	ADJ
aiua-14101	301	14	effect	effect	NOUN
aiua-14101	301	15	of	of	ADP
aiua-14101	301	16	trehalose	trehalose	PRON
aiua-14101	301	17	and	and	CCONJ
aiua-14101	301	18	low	low	ADJ
aiua-14101	301	19	-	-	PUNCT
aiua-14101	301	20	density	density	NOUN
aiua-14101	301	21	lipoprotein	lipoprotein	NOUN
aiua-14101	301	22	in	in	ADP
aiua-14101	301	23	extenders	extender	NOUN
aiua-14101	301	24	for	for	ADP
aiua-14101	301	25	frozen	frozen	ADJ
aiua-14101	301	26	ram	ram	NOUN
aiua-14101	301	27	semen	semen	NOUN
aiua-14101	301	28	.	.	PUNCT
aiua-14101	302	1	small	small	ADJ
aiua-14101	302	2	rumin	rumin	NOUN
aiua-14101	302	3	res	re	NOUN
aiua-14101	302	4	.	.	PUNCT
aiua-14101	302	5	2010	2010	NUM
aiua-14101	302	6	;	;	PUNCT
aiua-14101	302	7	93:206	93:206	NUM
aiua-14101	302	8	-	-	SYM
aiua-14101	302	9	9	9	NUM
aiua-14101	302	10	.	.	NOUN
aiua-14101	302	11	26	26	NUM
aiua-14101	302	12	.	.	PUNCT
aiua-14101	303	1	khalili	khalili	PROPN
aiua-14101	303	2	b	b	PROPN
aiua-14101	303	3	,	,	PUNCT
aiua-14101	303	4	farshad	farshad	VERB
aiua-14101	303	5	a	a	PRON
aiua-14101	303	6	,	,	PUNCT
aiua-14101	303	7	zamiri	zamiri	PROPN
aiua-14101	303	8	mj	mj	PROPN
aiua-14101	303	9	,	,	PUNCT
aiua-14101	303	10	et	et	PROPN
aiua-14101	303	11	al	al	PROPN
aiua-14101	303	12	.	.	PUNCT
aiua-14101	304	1	effects	effect	NOUN
aiua-14101	304	2	of	of	ADP
aiua-14101	304	3	sucrose	sucrose	NOUN
aiua-14101	304	4	and	and	CCONJ
aiua-14101	304	5	trehalose	trehalose	PRON
aiua-14101	304	6	on	on	ADP
aiua-14101	304	7	the	the	DET
aiua-14101	304	8	freezability	freezability	NOUN
aiua-14101	304	9	of	of	ADP
aiua-14101	304	10	markhoz	markhoz	PROPN
aiua-14101	304	11	goat	goat	PROPN
aiua-14101	304	12	spermatozoa	spermatozoa	PROPN
aiua-14101	304	13	.	.	PUNCT
aiua-14101	305	1	asianaustralas	asianaustralas	PROPN
aiua-14101	305	2	j	j	PROPN
aiua-14101	305	3	anim	anim	PROPN
aiua-14101	305	4	sci	sci	PROPN
aiua-14101	305	5	.	.	PROPN
aiua-14101	305	6	2009	2009	NUM
aiua-14101	305	7	;	;	PUNCT
aiua-14101	305	8	22:1614	22:1614	NUM
aiua-14101	305	9	-	-	SYM
aiua-14101	305	10	9	9	NUM
aiua-14101	305	11	.	.	NOUN
aiua-14101	305	12	27	27	NUM
aiua-14101	305	13	.	.	PUNCT
aiua-14101	305	14	koçak	koçak	PROPN
aiua-14101	305	15	g	g	PROPN
aiua-14101	305	16	,	,	PUNCT
aiua-14101	305	17	yildiz	yildiz	PROPN
aiua-14101	305	18	c.	c.	PROPN
aiua-14101	306	1	the	the	DET
aiua-14101	306	2	effects	effect	NOUN
aiua-14101	306	3	of	of	ADP
aiua-14101	306	4	ferulic	ferulic	ADJ
aiua-14101	306	5	acid	acid	NOUN
aiua-14101	306	6	,	,	PUNCT
aiua-14101	306	7	tryptophan	tryptophan	NOUN
aiua-14101	306	8	,	,	PUNCT
aiua-14101	306	9	and	and	CCONJ
aiua-14101	306	10	lglutamine	lglutamine	VERB
aiua-14101	306	11	on	on	ADP
aiua-14101	306	12	the	the	DET
aiua-14101	306	13	cryopreservation	cryopreservation	NOUN
aiua-14101	306	14	of	of	ADP
aiua-14101	306	15	mouse	mouse	NOUN
aiua-14101	306	16	spermatozoa	spermatozoon	NOUN
aiua-14101	306	17	.	.	PUNCT
aiua-14101	307	1	biopreserv	biopreserv	PROPN
aiua-14101	307	2	biobank	biobank	PROPN
aiua-14101	307	3	.	.	PUNCT
aiua-14101	308	1	2024	2024	NUM
aiua-14101	308	2	;	;	PUNCT
aiua-14101	308	3	22:286	22:286	NUM
aiua-14101	308	4	-	-	SYM
aiua-14101	308	5	93	93	NUM
aiua-14101	308	6	.	.	PUNCT
aiua-14101	309	1	28	28	NUM
aiua-14101	309	2	.	.	PUNCT
aiua-14101	310	1	aitken	aitken	PROPN
aiua-14101	310	2	rj	rj	PROPN
aiua-14101	310	3	,	,	PUNCT
aiua-14101	310	4	baker	baker	PROPN
aiua-14101	310	5	ma	ma	PROPN
aiua-14101	310	6	.	.	PROPN
aiua-14101	310	7	oxidative	oxidative	ADJ
aiua-14101	310	8	stress	stress	NOUN
aiua-14101	310	9	and	and	CCONJ
aiua-14101	310	10	male	male	ADJ
aiua-14101	310	11	reproductive	reproductive	ADJ
aiua-14101	310	12	biology	biology	NOUN
aiua-14101	310	13	.	.	PUNCT
aiua-14101	311	1	reprod	reprod	PROPN
aiua-14101	311	2	fertil	fertil	PROPN
aiua-14101	311	3	dev	dev	PROPN
aiua-14101	311	4	.	.	PROPN
aiua-14101	311	5	2004	2004	NUM
aiua-14101	311	6	;	;	PUNCT
aiua-14101	311	7	16:581	16:581	NUM
aiua-14101	311	8	-	-	SYM
aiua-14101	311	9	8	8	NUM
aiua-14101	311	10	.	.	NOUN
aiua-14101	311	11	29	29	NUM
aiua-14101	311	12	.	.	X
aiua-14101	312	1	aboagla	aboagla	PROPN
aiua-14101	312	2	eme	eme	PROPN
aiua-14101	312	3	,	,	PUNCT
aiua-14101	312	4	terada	terada	PROPN
aiua-14101	312	5	t.	t.	PROPN
aiua-14101	312	6	trehalose	trehalose	ADV
aiua-14101	312	7	-	-	PUNCT
aiua-14101	312	8	enhanced	enhance	VERB
aiua-14101	312	9	fluidity	fluidity	NOUN
aiua-14101	312	10	of	of	ADP
aiua-14101	312	11	the	the	DET
aiua-14101	312	12	goat	goat	PROPN
aiua-14101	312	13	sperm	sperm	NOUN
aiua-14101	312	14	membrane	membrane	NOUN
aiua-14101	312	15	and	and	CCONJ
aiua-14101	312	16	its	its	PRON
aiua-14101	312	17	protection	protection	NOUN
aiua-14101	312	18	during	during	ADP
aiua-14101	312	19	freezing	freeze	VERB
aiua-14101	312	20	.	.	PUNCT
aiua-14101	313	1	biol	biol	PROPN
aiua-14101	313	2	reprod	reprod	PROPN
aiua-14101	313	3	.	.	PUNCT
aiua-14101	313	4	2003	2003	NUM
aiua-14101	313	5	;	;	PUNCT
aiua-14101	313	6	69:1245	69:1245	NUM
aiua-14101	313	7	-	-	SYM
aiua-14101	313	8	50	50	NUM
aiua-14101	313	9	.	.	PUNCT
aiua-14101	314	1	30	30	NUM
aiua-14101	314	2	.	.	PUNCT
aiua-14101	315	1	hu	hu	PROPN
aiua-14101	315	2	jh	jh	PROPN
aiua-14101	315	3	,	,	PUNCT
aiua-14101	315	4	li	li	PROPN
aiua-14101	315	5	qw	qw	PROPN
aiua-14101	315	6	,	,	PUNCT
aiua-14101	315	7	li	li	PROPN
aiua-14101	315	8	g	g	PROPN
aiua-14101	315	9	,	,	PUNCT
aiua-14101	315	10	et	et	PROPN
aiua-14101	315	11	al	al	PROPN
aiua-14101	315	12	.	.	PUNCT
aiua-14101	316	1	the	the	DET
aiua-14101	316	2	cryoprotective	cryoprotective	ADJ
aiua-14101	316	3	effect	effect	NOUN
aiua-14101	316	4	of	of	ADP
aiua-14101	316	5	trehalose	trehalose	ADJ
aiua-14101	316	6	supplementation	supplementation	NOUN
aiua-14101	316	7	on	on	ADP
aiua-14101	316	8	boar	boar	NOUN
aiua-14101	316	9	spermatozoa	spermatozoa	NOUN
aiua-14101	316	10	quality	quality	NOUN
aiua-14101	316	11	.	.	PUNCT
aiua-14101	317	1	anim	anim	PROPN
aiua-14101	317	2	reprod	reprod	PROPN
aiua-14101	317	3	sci	sci	PROPN
aiua-14101	317	4	.	.	PROPN
aiua-14101	317	5	2009	2009	NUM
aiua-14101	317	6	;	;	PUNCT
aiua-14101	317	7	112:107	112:107	X
aiua-14101	317	8	-	-	SYM
aiua-14101	317	9	18	18	NUM
aiua-14101	317	10	.	.	PUNCT
aiua-14101	317	11	31	31	NUM
aiua-14101	317	12	.	.	PUNCT
aiua-14101	318	1	aisen	aisen	PROPN
aiua-14101	318	2	eg	eg	PROPN
aiua-14101	318	3	,	,	PUNCT
aiua-14101	318	4	medina	medina	PROPN
aiua-14101	318	5	vh	vh	PROPN
aiua-14101	318	6	,	,	PUNCT
aiua-14101	318	7	venturino	venturino	NOUN
aiua-14101	318	8	a.	a.	NOUN
aiua-14101	318	9	cryopreservation	cryopreservation	NOUN
aiua-14101	318	10	and	and	CCONJ
aiua-14101	318	11	postthawed	postthawe	VERB
aiua-14101	318	12	fertility	fertility	NOUN
aiua-14101	318	13	of	of	ADP
aiua-14101	318	14	ram	ram	NOUN
aiua-14101	318	15	semen	semen	NOUN
aiua-14101	318	16	frozen	freeze	VERB
aiua-14101	318	17	in	in	ADP
aiua-14101	318	18	different	different	ADJ
aiua-14101	318	19	trehalose	trehalose	ADJ
aiua-14101	318	20	concentrations	concentration	NOUN
aiua-14101	318	21	.	.	PUNCT
aiua-14101	319	1	theriogenology	theriogenology	NOUN
aiua-14101	319	2	.	.	PUNCT
aiua-14101	320	1	2002	2002	NUM
aiua-14101	320	2	;	;	PUNCT
aiua-14101	320	3	57:1801	57:1801	NUM
aiua-14101	320	4	-	-	SYM
aiua-14101	320	5	8	8	NUM
aiua-14101	320	6	.	.	PUNCT
aiua-14101	321	1	32	32	NUM
aiua-14101	321	2	.	.	PUNCT
aiua-14101	322	1	suksai	suksai	PROPN
aiua-14101	322	2	m	m	PROPN
aiua-14101	322	3	,	,	PUNCT
aiua-14101	322	4	dhanaworavibul	dhanaworavibul	VERB
aiua-14101	322	5	k.	k.	PROPN
aiua-14101	322	6	effects	effect	NOUN
aiua-14101	322	7	of	of	ADP
aiua-14101	322	8	trehalose	trehalose	NOUN
aiua-14101	322	9	and	and	CCONJ
aiua-14101	322	10	sucrose	sucrose	VERB
aiua-14101	322	11	on	on	ADP
aiua-14101	322	12	human	human	ADJ
aiua-14101	322	13	sperm	sperm	NOUN
aiua-14101	322	14	motility	motility	NOUN
aiua-14101	322	15	,	,	PUNCT
aiua-14101	322	16	vitality	vitality	NOUN
aiua-14101	322	17	and	and	CCONJ
aiua-14101	322	18	morphology	morphology	NOUN
aiua-14101	322	19	after	after	ADP
aiua-14101	322	20	cryopreservation	cryopreservation	NOUN
aiua-14101	322	21	.	.	PUNCT
aiua-14101	323	1	j	j	PROPN
aiua-14101	323	2	health	health	PROPN
aiua-14101	323	3	sci	sci	PROPN
aiua-14101	323	4	med	med	PROPN
aiua-14101	323	5	res	re	NOUN
aiua-14101	323	6	.	.	PROPN
aiua-14101	323	7	2019	2019	NUM
aiua-14101	323	8	;	;	PUNCT
aiua-14101	323	9	37:101	37:101	NUM
aiua-14101	323	10	-	-	SYM
aiua-14101	323	11	7	7	NUM
aiua-14101	323	12	.	.	NUM
aiua-14101	323	13	33	33	NUM
aiua-14101	323	14	..	..	PUNCT
aiua-14101	323	15	junior	junior	ADJ
aiua-14101	323	16	as	as	ADP
aiua-14101	323	17	,	,	PUNCT
aiua-14101	323	18	jardim	jardim	PROPN
aiua-14101	323	19	rd	rd	PROPN
aiua-14101	323	20	,	,	PUNCT
aiua-14101	323	21	streit	streit	PROPN
aiua-14101	323	22	dp	dp	PROPN
aiua-14101	323	23	,	,	PUNCT
aiua-14101	323	24	et	et	PROPN
aiua-14101	323	25	al	al	PROPN
aiua-14101	323	26	.	.	PROPN
aiua-14101	324	1	trehalose	trehalose	PROPN
aiua-14101	324	2	in	in	ADP
aiua-14101	324	3	extenders	extender	NOUN
aiua-14101	324	4	for	for	ADP
aiua-14101	324	5	cryopreservation	cryopreservation	NOUN
aiua-14101	324	6	of	of	ADP
aiua-14101	324	7	tambaqui	tambaqui	NOUN
aiua-14101	324	8	(	(	PUNCT
aiua-14101	324	9	colossoma	colossoma	NOUN
aiua-14101	324	10	macropomum	macropomum	ADJ
aiua-14101	324	11	)	)	PUNCT
aiua-14101	324	12	sperm	sperm	NOUN
aiua-14101	324	13	.	.	PUNCT
aiua-14101	325	1	cryo	cryo	NOUN
aiua-14101	325	2	letters	letter	NOUN
aiua-14101	325	3	.	.	PUNCT
aiua-14101	326	1	2022	2022	NUM
aiua-14101	326	2	;	;	PUNCT
aiua-14101	326	3	43:264	43:264	NOUN
aiua-14101	326	4	-	-	SYM
aiua-14101	326	5	8	8	NUM
aiua-14101	326	6	.	.	PUNCT
aiua-14101	327	1	34	34	NUM
aiua-14101	327	2	.	.	PUNCT
aiua-14101	328	1	kutluyer	kutluyer	PROPN
aiua-14101	328	2	f.	f.	PROPN
aiua-14101	328	3	in	in	ADP
aiua-14101	328	4	vitro	vitro	X
aiua-14101	328	5	effect	effect	NOUN
aiua-14101	328	6	of	of	ADP
aiua-14101	328	7	l	l	NOUN
aiua-14101	328	8	-	-	NOUN
aiua-14101	328	9	tryptophan	tryptophan	NOUN
aiua-14101	328	10	on	on	ADP
aiua-14101	328	11	the	the	DET
aiua-14101	328	12	quality	quality	NOUN
aiua-14101	328	13	and	and	CCONJ
aiua-14101	328	14	fertilizing	fertilizing	NOUN
aiua-14101	328	15	capacity	capacity	NOUN
aiua-14101	328	16	of	of	ADP
aiua-14101	328	17	sperms	sperm	NOUN
aiua-14101	328	18	of	of	ADP
aiua-14101	328	19	endangered	endanger	VERB
aiua-14101	328	20	species	specie	NOUN
aiua-14101	328	21	of	of	ADP
aiua-14101	328	22	trouts	trouts	PROPN
aiua-14101	328	23	.	.	PUNCT
aiua-14101	329	1	pak	pak	PROPN
aiua-14101	329	2	j	j	PROPN
aiua-14101	329	3	zool	zool	PROPN
aiua-14101	329	4	.	.	PROPN
aiua-14101	329	5	2018	2018	NUM
aiua-14101	329	6	;	;	PUNCT
aiua-14101	329	7	50:903	50:903	NUM
aiua-14101	329	8	-	-	SYM
aiua-14101	329	9	10	10	NUM
aiua-14101	329	10	.	.	PUNCT
aiua-14101	329	11	35	35	NUM
aiua-14101	329	12	.	.	PUNCT
aiua-14101	330	1	zhu	zhu	PROPN
aiua-14101	331	1	z	z	X
aiua-14101	331	2	,	,	PUNCT
aiua-14101	331	3	fan	fan	PROPN
aiua-14101	331	4	x	x	NOUN
aiua-14101	331	5	,	,	PUNCT
aiua-14101	331	6	pan	pan	PROPN
aiua-14101	331	7	y	y	PROPN
aiua-14101	331	8	,	,	PUNCT
aiua-14101	331	9	et	et	PROPN
aiua-14101	331	10	al	al	PROPN
aiua-14101	331	11	.	.	PUNCT
aiua-14101	332	1	trehalose	trehalose	PROPN
aiua-14101	332	2	improves	improve	VERB
aiua-14101	332	3	rabbit	rabbit	NOUN
aiua-14101	332	4	sperm	sperm	NOUN
aiua-14101	332	5	quality	quality	NOUN
aiua-14101	332	6	during	during	ADP
aiua-14101	332	7	cryopreservation	cryopreservation	NOUN
aiua-14101	332	8	.	.	PUNCT
aiua-14101	333	1	cryobiology	cryobiology	NOUN
aiua-14101	333	2	.	.	PUNCT
aiua-14101	334	1	2017	2017	NUM
aiua-14101	334	2	;	;	PUNCT
aiua-14101	334	3	75:45	75:45	NUM
aiua-14101	334	4	-	-	SYM
aiua-14101	334	5	51	51	NUM
aiua-14101	334	6	.	.	PUNCT
aiua-14101	335	1	36	36	NUM
aiua-14101	335	2	.	.	PUNCT
aiua-14101	336	1	ahmed	ahmed	PROPN
aiua-14101	336	2	h	h	PROPN
aiua-14101	336	3	,	,	PUNCT
aiua-14101	336	4	jahan	jahan	PROPN
aiua-14101	336	5	s	s	PROPN
aiua-14101	336	6	,	,	PUNCT
aiua-14101	336	7	khan	khan	PROPN
aiua-14101	336	8	a	a	PROPN
aiua-14101	336	9	,	,	PUNCT
aiua-14101	336	10	et	et	PROPN
aiua-14101	336	11	al	al	PROPN
aiua-14101	336	12	.	.	PROPN
aiua-14101	336	13	supplementation	supplementation	NOUN
aiua-14101	336	14	of	of	ADP
aiua-14101	336	15	l	l	NOUN
aiua-14101	336	16	-	-	NOUN
aiua-14101	336	17	tryptophan	tryptophan	NOUN
aiua-14101	336	18	(	(	PUNCT
aiua-14101	336	19	an	an	DET
aiua-14101	336	20	aromatic	aromatic	ADJ
aiua-14101	336	21	amino	amino	NOUN
aiua-14101	336	22	acid	acid	NOUN
aiua-14101	336	23	)	)	PUNCT
aiua-14101	336	24	in	in	ADP
aiua-14101	336	25	tris	tris	ADJ
aiua-14101	336	26	citric	citric	ADJ
aiua-14101	336	27	acid	acid	NOUN
aiua-14101	336	28	extender	extender	NOUN
aiua-14101	336	29	enhances	enhance	VERB
aiua-14101	336	30	post	post	ADJ
aiua-14101	336	31	-	-	ADJ
aiua-14101	336	32	thaw	thaw	ADJ
aiua-14101	336	33	progressive	progressive	ADJ
aiua-14101	336	34	motility	motility	NOUN
aiua-14101	336	35	,	,	PUNCT
aiua-14101	336	36	plasmalemma	plasmalemma	ADJ
aiua-14101	336	37	,	,	PUNCT
aiua-14101	336	38	mitochondrial	mitochondrial	ADJ
aiua-14101	336	39	membrane	membrane	NOUN
aiua-14101	336	40	potential	potential	NOUN
aiua-14101	336	41	,	,	PUNCT
aiua-14101	336	42	acrosome	acrosome	ADV
aiua-14101	336	43	,	,	PUNCT
aiua-14101	336	44	and	and	CCONJ
aiua-14101	336	45	dna	dna	PROPN
aiua-14101	336	46	integrities	integrity	NOUN
aiua-14101	336	47	,	,	PUNCT
aiua-14101	336	48	and	and	CCONJ
aiua-14101	336	49	in	in	ADP
aiua-14101	336	50	vivo	vivo	ADJ
aiua-14101	336	51	fertility	fertility	NOUN
aiua-14101	336	52	rate	rate	NOUN
aiua-14101	336	53	of	of	ADP
aiua-14101	336	54	buffalo	buffalo	PROPN
aiua-14101	336	55	(	(	PUNCT
aiua-14101	336	56	bubalus	bubalus	ADJ
aiua-14101	336	57	bubalis	bubalis	PROPN
aiua-14101	336	58	)	)	PUNCT
aiua-14101	336	59	bull	bull	NOUN
aiua-14101	336	60	spermatozoa	spermatozoon	NOUN
aiua-14101	336	61	.	.	PUNCT
aiua-14101	337	1	cryobiology	cryobiology	NOUN
aiua-14101	337	2	.	.	PUNCT
aiua-14101	338	1	2020	2020	NUM
aiua-14101	338	2	;	;	PUNCT
aiua-14101	338	3	92:117	92:117	NUM
aiua-14101	338	4	-	-	SYM
aiua-14101	338	5	23	23	NUM
aiua-14101	338	6	.	.	PUNCT
aiua-14101	339	1	37	37	NUM
aiua-14101	339	2	.	.	PUNCT
aiua-14101	339	3	white	white	PROPN
aiua-14101	339	4	ig	ig	PROPN
aiua-14101	339	5	.	.	PUNCT
aiua-14101	340	1	lipids	lipids	PROPN
aiua-14101	340	2	and	and	CCONJ
aiua-14101	340	3	calcium	calcium	NOUN
aiua-14101	340	4	uptake	uptake	NOUN
aiua-14101	340	5	of	of	ADP
aiua-14101	340	6	sperm	sperm	NOUN
aiua-14101	340	7	in	in	ADP
aiua-14101	340	8	relation	relation	NOUN
aiua-14101	340	9	to	to	ADP
aiua-14101	340	10	cold	cold	ADJ
aiua-14101	340	11	shock	shock	NOUN
aiua-14101	340	12	and	and	CCONJ
aiua-14101	340	13	preservation	preservation	NOUN
aiua-14101	340	14	:	:	PUNCT
aiua-14101	340	15	a	a	DET
aiua-14101	340	16	review	review	NOUN
aiua-14101	340	17	.	.	PUNCT
aiua-14101	341	1	reprod	reprod	PROPN
aiua-14101	341	2	fertil	fertil	PROPN
aiua-14101	341	3	dev	dev	PROPN
aiua-14101	341	4	.	.	PROPN
aiua-14101	341	5	1993	1993	NUM
aiua-14101	341	6	;	;	PUNCT
aiua-14101	341	7	5:639	5:639	NUM
aiua-14101	341	8	-	-	SYM
aiua-14101	341	9	58	58	NUM
aiua-14101	341	10	.	.	PUNCT
aiua-14101	342	1	38	38	NUM
aiua-14101	342	2	.	.	PUNCT
aiua-14101	343	1	jafaroghli	jafaroghli	PROPN
aiua-14101	343	2	m	m	PROPN
aiua-14101	343	3	,	,	PUNCT
aiua-14101	343	4	khalili	khalili	PROPN
aiua-14101	343	5	b	b	PROPN
aiua-14101	343	6	,	,	PUNCT
aiua-14101	343	7	farshad	farshad	VERB
aiua-14101	343	8	a	a	PRON
aiua-14101	343	9	,	,	PUNCT
aiua-14101	343	10	et	et	PROPN
aiua-14101	343	11	al	al	PROPN
aiua-14101	343	12	.	.	PUNCT
aiua-14101	344	1	the	the	DET
aiua-14101	344	2	effect	effect	NOUN
aiua-14101	344	3	of	of	ADP
aiua-14101	344	4	supplementation	supplementation	NOUN
aiua-14101	344	5	of	of	ADP
aiua-14101	344	6	cryopreservation	cryopreservation	NOUN
aiua-14101	344	7	diluents	diluent	NOUN
aiua-14101	344	8	with	with	ADP
aiua-14101	344	9	sugars	sugar	NOUN
aiua-14101	344	10	on	on	ADP
aiua-14101	344	11	the	the	DET
aiua-14101	344	12	post	post	ADJ
aiua-14101	344	13	-	-	ADJ
aiua-14101	344	14	thawing	thaw	VERB
aiua-14101	344	15	fertility	fertility	NOUN
aiua-14101	344	16	of	of	ADP
aiua-14101	344	17	ram	ram	NOUN
aiua-14101	344	18	semen	semen	NOUN
aiua-14101	344	19	.	.	PUNCT
aiua-14101	345	1	small	small	ADJ
aiua-14101	345	2	rumin	rumin	NOUN
aiua-14101	345	3	res	re	NOUN
aiua-14101	345	4	.	.	PUNCT
aiua-14101	345	5	2011	2011	NUM
aiua-14101	345	6	;	;	PUNCT
aiua-14101	345	7	96:58	96:58	NUM
aiua-14101	345	8	-	-	SYM
aiua-14101	345	9	63	63	NUM
aiua-14101	345	10	.	.	PUNCT
aiua-14101	345	11	39	39	NUM
aiua-14101	345	12	.	.	PUNCT
aiua-14101	346	1	hu	hu	PROPN
aiua-14101	346	2	jh	jh	PROPN
aiua-14101	346	3	,	,	PUNCT
aiua-14101	346	4	zan	zan	PROPN
aiua-14101	346	5	ls	ls	PROPN
aiua-14101	346	6	,	,	PUNCT
aiua-14101	346	7	zhao	zhao	PROPN
aiua-14101	346	8	xl	xl	PROPN
aiua-14101	346	9	,	,	PUNCT
aiua-14101	346	10	et	et	PROPN
aiua-14101	346	11	al	al	PROPN
aiua-14101	346	12	.	.	PUNCT
aiua-14101	346	13	effects	effect	NOUN
aiua-14101	346	14	of	of	ADP
aiua-14101	346	15	trehalose	trehalose	ADJ
aiua-14101	346	16	supplementation	supplementation	NOUN
aiua-14101	346	17	on	on	ADP
aiua-14101	346	18	semen	semen	NOUN
aiua-14101	346	19	quality	quality	NOUN
aiua-14101	346	20	and	and	CCONJ
aiua-14101	346	21	oxidative	oxidative	ADJ
aiua-14101	346	22	stress	stress	NOUN
aiua-14101	346	23	variables	variable	NOUN
aiua-14101	346	24	in	in	ADP
aiua-14101	346	25	frozen	frozen	ADJ
aiua-14101	346	26	-	-	PUNCT
aiua-14101	346	27	thawed	thaw	VERB
aiua-14101	346	28	bovine	bovine	NOUN
aiua-14101	346	29	semen	semen	NOUN
aiua-14101	346	30	.	.	PUNCT
aiua-14101	347	1	j	j	PROPN
aiua-14101	347	2	anim	anim	PROPN
aiua-14101	347	3	sci	sci	PROPN
aiua-14101	347	4	.	.	PROPN
aiua-14101	347	5	2010	2010	NUM
aiua-14101	347	6	;	;	PUNCT
aiua-14101	347	7	88:1657	88:1657	NOUN
aiua-14101	347	8	-	-	PUNCT
aiua-14101	347	9	62	62	NUM
aiua-14101	347	10	.	.	PUNCT
aiua-14101	348	1	40	40	NUM
aiua-14101	348	2	.	.	PUNCT
aiua-14101	349	1	gholami	gholami	PROPN
aiua-14101	349	2	d	d	NOUN
aiua-14101	349	3	,	,	PUNCT
aiua-14101	349	4	sharafi	sharafi	PROPN
aiua-14101	349	5	m	m	PROPN
aiua-14101	349	6	,	,	PUNCT
aiua-14101	349	7	esmaeili	esmaeili	NOUN
aiua-14101	349	8	v	v	NOUN
aiua-14101	349	9	,	,	PUNCT
aiua-14101	349	10	et	et	PROPN
aiua-14101	349	11	al	al	PROPN
aiua-14101	349	12	.	.	PROPN
aiua-14101	349	13	beneficial	beneficial	ADJ
aiua-14101	349	14	effects	effect	NOUN
aiua-14101	349	15	of	of	ADP
aiua-14101	349	16	trehalose	trehalose	NOUN
aiua-14101	349	17	and	and	CCONJ
aiua-14101	349	18	gentiobiose	gentiobiose	VERB
aiua-14101	349	19	on	on	ADP
aiua-14101	349	20	human	human	ADJ
aiua-14101	349	21	sperm	sperm	NOUN
aiua-14101	349	22	cryopreservation	cryopreservation	NOUN
aiua-14101	349	23	.	.	PUNCT
aiua-14101	350	1	plos	plos	PROPN
aiua-14101	350	2	one	one	NUM
aiua-14101	350	3	.	.	PUNCT
aiua-14101	351	1	2023	2023	NUM
aiua-14101	351	2	;	;	PUNCT
aiua-14101	351	3	18	18	NUM
aiua-14101	351	4	:	:	PUNCT
aiua-14101	351	5	e0271210	e0271210	PROPN
aiua-14101	351	6	.	.	PROPN
aiua-14101	351	7	41	41	NUM
aiua-14101	351	8	.	.	PUNCT
aiua-14101	352	1	xie	xie	PROPN
aiua-14101	352	2	l	l	PROPN
aiua-14101	352	3	,	,	PUNCT
aiua-14101	352	4	ding	de	VERB
aiua-14101	352	5	y	y	PROPN
aiua-14101	352	6	,	,	PUNCT
aiua-14101	352	7	xu	xu	PROPN
aiua-14101	353	1	p	p	X
aiua-14101	353	2	,	,	PUNCT
aiua-14101	353	3	et	et	PROPN
aiua-14101	353	4	al	al	PROPN
aiua-14101	353	5	.	.	PUNCT
aiua-14101	353	6	effects	effect	NOUN
aiua-14101	353	7	of	of	ADP
aiua-14101	353	8	sucrose	sucrose	PROPN
aiua-14101	353	9	,	,	PUNCT
aiua-14101	353	10	trehalose	trehalose	ADV
aiua-14101	353	11	,	,	PUNCT
aiua-14101	353	12	and	and	CCONJ
aiua-14101	353	13	fructose	fructose	NOUN
aiua-14101	353	14	on	on	ADP
aiua-14101	353	15	whole	whole	ADJ
aiua-14101	353	16	ovarian	ovarian	ADJ
aiua-14101	353	17	cryopreservation	cryopreservation	NOUN
aiua-14101	353	18	in	in	ADP
aiua-14101	353	19	a	a	DET
aiua-14101	353	20	rat	rat	NOUN
aiua-14101	353	21	model	model	NOUN
aiua-14101	353	22	.	.	PUNCT
aiua-14101	354	1	clin	clin	PROPN
aiua-14101	354	2	exp	exp	NOUN
aiua-14101	354	3	obstet	obstet	NOUN
aiua-14101	354	4	gynecol	gynecol	PROPN
aiua-14101	354	5	.	.	PUNCT
aiua-14101	354	6	2024	2024	NUM
aiua-14101	354	7	;	;	PUNCT
aiua-14101	354	8	51:90	51:90	NUM
aiua-14101	354	9	.	.	PUNCT
aiua-14101	355	1	42	42	NUM
aiua-14101	355	2	.	.	PUNCT
aiua-14101	355	3	du	du	PROPN
aiua-14101	355	4	t	t	PROPN
aiua-14101	355	5	,	,	PUNCT
aiua-14101	355	6	chao	chao	PROPN
aiua-14101	355	7	l	l	PROPN
aiua-14101	355	8	,	,	PUNCT
aiua-14101	355	9	zhao	zhao	PROPN
aiua-14101	355	10	s	s	PROPN
aiua-14101	355	11	,	,	PUNCT
aiua-14101	355	12	et	et	PROPN
aiua-14101	355	13	al	al	PROPN
aiua-14101	355	14	.	.	PROPN
aiua-14101	356	1	successful	successful	ADJ
aiua-14101	356	2	cryopreservation	cryopreservation	NOUN
aiua-14101	356	3	of	of	ADP
aiua-14101	356	4	whole	whole	ADJ
aiua-14101	356	5	sheep	sheep	NOUN
aiua-14101	356	6	ovary	ovary	ADJ
aiua-14101	356	7	by	by	ADP
aiua-14101	356	8	using	use	VERB
aiua-14101	356	9	dmso	dmso	NOUN
aiua-14101	356	10	-	-	PUNCT
aiua-14101	356	11	free	free	ADJ
aiua-14101	356	12	cryoprotectant	cryoprotectant	NOUN
aiua-14101	356	13	.	.	PUNCT
aiua-14101	357	1	j	j	PROPN
aiua-14101	357	2	assist	assist	VERB
aiua-14101	357	3	reprod	reprod	PROPN
aiua-14101	357	4	genet	genet	PROPN
aiua-14101	357	5	.	.	PUNCT
aiua-14101	358	1	2015	2015	NUM
aiua-14101	358	2	;	;	PUNCT
aiua-14101	358	3	32:1267	32:1267	NUM
aiua-14101	358	4	-	-	SYM
aiua-14101	358	5	75	75	NUM
aiua-14101	358	6	.	.	PUNCT
aiua-14101	359	1	43	43	NUM
aiua-14101	359	2	.	.	PUNCT
aiua-14101	360	1	izaguirre	izaguirre	PROPN
aiua-14101	360	2	-	-	PUNCT
aiua-14101	360	3	pérez	pérez	PROPN
aiua-14101	360	4	n	n	CCONJ
aiua-14101	360	5	,	,	PUNCT
aiua-14101	360	6	ligero	ligero	PROPN
aiua-14101	360	7	g	g	PROPN
aiua-14101	360	8	,	,	PUNCT
aiua-14101	360	9	aguilar	aguilar	PROPN
aiua-14101	360	10	-	-	PUNCT
aiua-14101	360	11	solana	solana	PROPN
aiua-14101	360	12	pa	pa	PROPN
aiua-14101	360	13	,	,	PUNCT
aiua-14101	360	14	et	et	PROPN
aiua-14101	360	15	al	al	PROPN
aiua-14101	360	16	.	.	PROPN
aiua-14101	361	1	trehalose	trehalose	PROPN
aiua-14101	361	2	cryopreservation	cryopreservation	NOUN
aiua-14101	361	3	of	of	ADP
aiua-14101	361	4	human	human	ADJ
aiua-14101	361	5	mesenchymal	mesenchymal	NOUN
aiua-14101	361	6	stem	stem	NOUN
aiua-14101	361	7	cells	cell	NOUN
aiua-14101	361	8	from	from	ADP
aiua-14101	361	9	cord	cord	NOUN
aiua-14101	361	10	tissue	tissue	NOUN
aiua-14101	361	11	.	.	PUNCT
aiua-14101	362	1	biopreserv	biopreserv	PROPN
aiua-14101	362	2	biobank	biobank	PROPN
aiua-14101	362	3	.	.	PUNCT
aiua-14101	363	1	2024	2024	NUM
aiua-14101	363	2	;	;	PUNCT
aiua-14101	363	3	22:294	22:294	NUM
aiua-14101	363	4	-	-	SYM
aiua-14101	363	5	302	302	NUM
aiua-14101	363	6	.	.	PUNCT
aiua-14101	364	1	44	44	NUM
aiua-14101	364	2	.	.	PUNCT
aiua-14101	365	1	lee	lee	PROPN
aiua-14101	365	2	ya	ya	PROPN
aiua-14101	365	3	,	,	PUNCT
aiua-14101	365	4	kim	kim	PROPN
aiua-14101	365	5	yh	yh	PROPN
aiua-14101	365	6	,	,	PUNCT
aiua-14101	365	7	kim	kim	PROPN
aiua-14101	365	8	bs	bs	PROPN
aiua-14101	365	9	,	,	PUNCT
aiua-14101	365	10	et	et	PROPN
aiua-14101	365	11	al	al	PROPN
aiua-14101	365	12	.	.	PUNCT
aiua-14101	365	13	cryopreservation	cryopreservation	NOUN
aiua-14101	365	14	in	in	ADP
aiua-14101	365	15	trehalose	trehalose	PRON
aiua-14101	365	16	preserves	preserve	NOUN
aiua-14101	365	17	functional	functional	ADJ
aiua-14101	365	18	capacity	capacity	NOUN
aiua-14101	365	19	of	of	ADP
aiua-14101	365	20	murine	murine	ADJ
aiua-14101	365	21	spermatogonial	spermatogonial	ADJ
aiua-14101	365	22	stem	stem	NOUN
aiua-14101	365	23	cells	cell	NOUN
aiua-14101	365	24	.	.	PUNCT
aiua-14101	366	1	plos	plos	PROPN
aiua-14101	366	2	one	one	NUM
aiua-14101	366	3	.	.	PUNCT
aiua-14101	367	1	2013	2013	NUM
aiua-14101	367	2	;	;	PUNCT
aiua-14101	367	3	8	8	NUM
aiua-14101	367	4	:	:	PUNCT
aiua-14101	367	5	e54889	e54889	VERB
aiua-14101	367	6	.	.	PUNCT
aiua-14101	368	1	45	45	NUM
aiua-14101	368	2	.	.	PUNCT
aiua-14101	369	1	ibrahim	ibrahim	PROPN
aiua-14101	369	2	s	s	PROPN
aiua-14101	369	3	,	,	PUNCT
aiua-14101	369	4	shin	shin	PROPN
aiua-14101	369	5	s	s	PROPN
aiua-14101	369	6	,	,	PUNCT
aiua-14101	369	7	talha	talha	PROPN
aiua-14101	369	8	nah	nah	INTJ
aiua-14101	369	9	,	,	PUNCT
aiua-14101	369	10	et	et	PROPN
aiua-14101	369	11	al	al	PROPN
aiua-14101	369	12	.	.	PROPN
aiua-14101	369	13	effect	effect	NOUN
aiua-14101	369	14	of	of	ADP
aiua-14101	369	15	trehalose	trehalose	ADJ
aiua-14101	369	16	supplementation	supplementation	NOUN
aiua-14101	369	17	in	in	ADP
aiua-14101	369	18	egg	egg	NOUN
aiua-14101	369	19	-	-	PUNCT
aiua-14101	369	20	yolk	yolk	NOUN
aiua-14101	369	21	-	-	PUNCT
aiua-14101	369	22	free	free	ADJ
aiua-14101	369	23	extender	extender	NOUN
aiua-14101	369	24	on	on	ADP
aiua-14101	369	25	conventional	conventional	ADJ
aiua-14101	369	26	parameters	parameter	NOUN
aiua-14101	369	27	and	and	CCONJ
aiua-14101	369	28	gene	gene	NOUN
aiua-14101	369	29	expression	expression	NOUN
aiua-14101	369	30	related	relate	VERB
aiua-14101	369	31	to	to	ADP
aiua-14101	369	32	reactive	reactive	ADJ
aiua-14101	369	33	oxygen	oxygen	NOUN
aiua-14101	369	34	species	specie	NOUN
aiua-14101	369	35	,	,	PUNCT
aiua-14101	369	36	apoptosis	apoptosis	NOUN
aiua-14101	369	37	,	,	PUNCT
aiua-14101	369	38	and	and	CCONJ
aiua-14101	369	39	motility	motility	NOUN
aiua-14101	369	40	of	of	ADP
aiua-14101	369	41	frozen	frozen	ADJ
aiua-14101	369	42	dog	dog	NOUN
aiua-14101	369	43	spermatozoa	spermatozoon	NOUN
aiua-14101	369	44	.	.	PUNCT
aiua-14101	370	1	biopreserv	biopreserv	PROPN
aiua-14101	370	2	biobank	biobank	PROPN
aiua-14101	370	3	.	.	PUNCT
aiua-14101	371	1	2024	2024	NUM
aiua-14101	371	2	;	;	PUNCT
aiua-14101	371	3	22:395	22:395	NUM
aiua-14101	371	4	-	-	SYM
aiua-14101	371	5	403	403	NUM
aiua-14101	371	6	.	.	PUNCT
aiua-14101	372	1	46	46	NUM
aiua-14101	372	2	.	.	PUNCT
aiua-14101	373	1	bumbat	bumbat	PROPN
aiua-14101	373	2	m	m	PROPN
aiua-14101	373	3	,	,	PUNCT
aiua-14101	373	4	wang	wang	PROPN
aiua-14101	373	5	m	m	PROPN
aiua-14101	373	6	,	,	PUNCT
aiua-14101	373	7	liang	liang	PROPN
aiua-14101	373	8	w	w	PROPN
aiua-14101	373	9	,	,	PUNCT
aiua-14101	373	10	et	et	PROPN
aiua-14101	373	11	al	al	PROPN
aiua-14101	373	12	.	.	PUNCT
aiua-14101	373	13	effects	effect	NOUN
aiua-14101	373	14	of	of	ADP
aiua-14101	373	15	me2so	me2so	NOUN
aiua-14101	373	16	and	and	CCONJ
aiua-14101	373	17	trehalose	trehalose	NOUN
aiua-14101	373	18	on	on	ADP
aiua-14101	373	19	the	the	DET
aiua-14101	373	20	cell	cell	NOUN
aiua-14101	373	21	viability	viability	NOUN
aiua-14101	373	22	,	,	PUNCT
aiua-14101	373	23	proliferation	proliferation	NOUN
aiua-14101	373	24	,	,	PUNCT
aiua-14101	373	25	and	and	CCONJ
aiua-14101	373	26	bcl-2	bcl-2	VERB
aiua-14101	373	27	family	family	NOUN
aiua-14101	373	28	gene	gene	NOUN
aiua-14101	373	29	(	(	PUNCT
aiua-14101	373	30	bcl2	bcl2	PROPN
aiua-14101	373	31	bax	bax	PROPN
aiua-14101	373	32	,	,	PUNCT
aiua-14101	373	33	and	and	CCONJ
aiua-14101	373	34	bad	bad	ADJ
aiua-14101	373	35	)	)	PUNCT
aiua-14101	373	36	expression	expression	NOUN
aiua-14101	373	37	in	in	ADP
aiua-14101	373	38	cryopreserved	cryopreserve	VERB
aiua-14101	373	39	human	human	ADJ
aiua-14101	373	40	breast	breast	NOUN
aiua-14101	373	41	cancer	cancer	NOUN
aiua-14101	373	42	cells	cell	NOUN
aiua-14101	373	43	.	.	PUNCT
aiua-14101	374	1	biopreserv	biopreserv	PROPN
aiua-14101	374	2	biobank	biobank	PROPN
aiua-14101	374	3	.	.	PUNCT
aiua-14101	375	1	2020	2020	NUM
aiua-14101	375	2	;	;	PUNCT
aiua-14101	375	3	18:33	18:33	NUM
aiua-14101	375	4	-	-	SYM
aiua-14101	375	5	40	40	NUM
aiua-14101	375	6	.	.	PUNCT
aiua-14101	376	1	correspondence	correspondence	NOUN
aiua-14101	376	2	firdevs	firdev	VERB
aiua-14101	376	3	yilmaz	yilmaz	PROPN
aiua-14101	376	4	dayanc	dayanc	PROPN
aiua-14101	376	5	,	,	PUNCT
aiua-14101	376	6	ph.d	ph.d	PROPN
aiua-14101	376	7	.	.	PUNCT
aiua-14101	377	1	fiirdevsyiilmaz@gmail.com	fiirdevsyiilmaz@gmail.com	PROPN
aiua-14101	378	1	hatay	hatay	NOUN
aiua-14101	378	2	mustafa	mustafa	PROPN
aiua-14101	378	3	kemal	kemal	PROPN
aiua-14101	378	4	university	university	PROPN
aiua-14101	378	5	,	,	PUNCT
aiua-14101	378	6	campus	campus	NOUN
aiua-14101	378	7	of	of	ADP
aiua-14101	378	8	tayfur	tayfur	NOUN
aiua-14101	378	9	sokmen	sokman	NOUN
aiua-14101	378	10	,	,	PUNCT
aiua-14101	378	11	faculty	faculty	NOUN
aiua-14101	378	12	of	of	ADP
aiua-14101	378	13	veterinary	veterinary	ADJ
aiua-14101	378	14	medicine	medicine	NOUN
aiua-14101	378	15	,	,	PUNCT
aiua-14101	378	16	department	department	NOUN
aiua-14101	378	17	of	of	ADP
aiua-14101	378	18	reproduction	reproduction	NOUN
aiua-14101	378	19	and	and	CCONJ
aiua-14101	378	20	artificial	artificial	ADJ
aiua-14101	378	21	insemination	insemination	NOUN
aiua-14101	378	22	,	,	PUNCT
aiua-14101	378	23	31060	31060	NUM
aiua-14101	378	24	,	,	PUNCT
aiua-14101	378	25	hatay	hatay	NOUN
aiua-14101	378	26	,	,	PUNCT
aiua-14101	378	27	turkey	turkey	PROPN
aiua-14101	378	28	oguz	oguz	PROPN
aiua-14101	378	29	kaan	kaan	PROPN
aiua-14101	378	30	yalcin	yalcin	PROPN
aiua-14101	378	31	okaanyalcin@gmail.com	okaanyalcin@gmail.com	PROPN
aiua-14101	379	1	hatay	hatay	NOUN
aiua-14101	379	2	mustafa	mustafa	PROPN
aiua-14101	379	3	kemal	kemal	PROPN
aiua-14101	379	4	university	university	PROPN
aiua-14101	379	5	,	,	PUNCT
aiua-14101	379	6	faculty	faculty	NOUN
aiua-14101	379	7	of	of	ADP
aiua-14101	379	8	veterinary	veterinary	ADJ
aiua-14101	379	9	medicine	medicine	NOUN
aiua-14101	379	10	,	,	PUNCT
aiua-14101	379	11	department	department	NOUN
aiua-14101	379	12	of	of	ADP
aiua-14101	379	13	reproduction	reproduction	NOUN
aiua-14101	379	14	and	and	CCONJ
aiua-14101	379	15	artificial	artificial	ADJ
aiua-14101	379	16	insemination	insemination	NOUN
aiua-14101	379	17	,	,	PUNCT
aiua-14101	379	18	hatay	hatay	NOUN
aiua-14101	379	19	,	,	PUNCT
aiua-14101	379	20	turkey	turkey	PROPN
aiua-14101	379	21	aysel	aysel	PROPN
aiua-14101	379	22	eraslan	eraslan	PROPN
aiua-14101	379	23	sakar	sakar	PROPN
aiua-14101	379	24	ayseleraslan@gmail.com	ayseleraslan@gmail.com	X
aiua-14101	379	25	hatay	hatay	PROPN
aiua-14101	379	26	mustafa	mustafa	PROPN
aiua-14101	379	27	kemal	kemal	PROPN
aiua-14101	379	28	university	university	PROPN
aiua-14101	379	29	,	,	PUNCT
aiua-14101	379	30	faculty	faculty	NOUN
aiua-14101	379	31	of	of	ADP
aiua-14101	379	32	veterinary	veterinary	ADJ
aiua-14101	379	33	medicine	medicine	NOUN
aiua-14101	379	34	,	,	PUNCT
aiua-14101	379	35	department	department	NOUN
aiua-14101	379	36	of	of	ADP
aiua-14101	379	37	genetics	genetics	PROPN
aiua-14101	379	38	,	,	PUNCT
aiua-14101	379	39	hatay	hatay	NOUN
aiua-14101	379	40	,	,	PUNCT
aiua-14101	379	41	turkey	turkey	NOUN