335Archivio Italiano di Urologia e Andrologia 2016; 88, 4 CASE REPORT Intraoperative ultrasound-guided enucleation of testicular nodule Riccardo Boschian 1, Giovanni Liguori 1, Stefano Bucci 1, Michele Bertolotto 1, Carlo Trombetta 1 Department of Urology and Radiology, University of Trieste, Trieste, Italy; Objective: We report a case of enucleation of a non-palpable right testicular lesion found incidentally at testicular ultrasonography during inves- tigations in a patient with azoospermia. Materials and methods: In 2011 bilateral hypoechoic non- palpable testicular lesions (5 mm and 3 mm to the right, 3 mm to the left) were found in a 28 years old patient, during diagnostic investigations for azoospermia. In March 2016, ultrasonography showed that the diameter of the right major nodule had grown to 12 mm, characterized by increased vascularization and increased texture. Blood exams showed serum FSH above normal levels with negative oncologic markers. The patients underwent surgical enucleation of the right nodule under ultrasonography guidance. Results: In post operative day 1 a control ultrasonography documented the disappearance of the lesion. Hystopathologic examination diagnosed a Leydig cell tumor, with negative surgical margins. The patient is in good clinical conditions and is under periodic ultrasonographic follow up. Conclusion: Organ sparing surgery represent a good thera- peutic option for little intraparenchymal lesions, mostly in young patients in which is preferable to preserve fertility. Intraoperatory ultrasonography represent an important tool for the localization of the lesion. KEY WORDS: Leydig cell tumor; Ultrasonography; Testis sparing surgery. Submitted 15 November 2016; Accepted 15 December 2016 Summary No conflict of interest declared. periodic ultrasonography follow up showed a progres- sive increase of the 5 mm right nodule. In march 2016, colorDoppler ultrasound showed that the diameter of the right nodule had grown to 12 mm, characterized by increased vascularization and increased texture to elas- tography. Blood exams showed serum FSH above normal levels but normal serum LH and testosterone, with neg- ative oncologic markers. Surgical technique: after right inguinal incision and access to the inguinal canal, the right spermatic cord was identified and isolated to the external inguinal ring, with subsequent exteriorization of the testicle. The testicular nodule was identified with intraoperative ultrasonogra- phy and marked with a needle. After incision of tunica albuginea, the nodule was resected by blunt dissection and the surgical specimen was sent to histopathological examination. The tunica albuginea was closed and after the eversion of the tunica vaginalis, the testicle was repo- sitioned in the scrotum. Intraoperative histological examination diagnosed a Leydig cell tumor. RESULTS The post operative course was regular, without compli- cations and the patient is in good clinical conditions. In post operative day 1 ultrasonography was performed with the evidence of the disappearance of the lesion in absence of intraparenchymal hematoma. Histopathologic exam diagnosed a Leydig cell tumor with surgical mar- gins free from disease. The tumor was immunopositive for Calretinin, Alpha-inhibin and Melan-A, with a weak positivity for p53. Because of the presence of bilateral lesions (of stable dimensions through the years) and the positivity for p53, the patients is actually under periodic ultrasound follow up. DISCUSSION Leydig cell tumours represent about 1-3% of adult tes- ticular tumours and are most common in the third to sixth decade in adults (1). Only 3% of Leydig cell tumours are bilateral and it is the benign definitive his- tology in 80% of the cases (1). Frequently its clinical presentation is asymptomatic with typical features as gynaecomastia, infertility or endocrinological disorders DOI: 10.4081/aiua.2016.4.335 Presented at 20th National Congress SIEUN, Sciacca 2016 CASE REPORT We report a case of resection of a non-palpable right tes- ticular lesion (histopathologic examination revealed a Leydig cell tumor) found incidentally at testicular ultra- sonography during investigations in a patient with azoospermia. MATERIALS AND METHODS A 28 years old patient underwent surgical enucleation under ultrasonography guidance of a 12 mm nodule to the right testicle. In 2011 bilateral hypoechoic non-palpable testicular lesions, respectively of 5 mm and 3 mm to the right and 3 mm to the left, were found in the patient during diag- nostic investigations for azoospermia. Through the years Boschian-Intraoperative ultrasound_Stesura Seveso 09/01/17 10:47 Pagina 335 Archivio Italiano di Urologia e Andrologia 2016; 88, 4 R. Boschian, G. Liguori, S. Bucci, M. Bertolotto, C. Trombetta 336 and it is often detected as a collateral finding during radi- ological investigations (mostly ultrasonography). Testis sparing surgery is highly recommended in every small, non palpable, ultrasound-detected nodule, in absence of rete testis invasion and with normal serum LH and testosterone (1, 2). If the histological examination reveals a malignant tumor, it is possible to perform a delayed orchidectomy. CONCLUSIONS Testis sparing surgery represent a good therapeutic option for little intraparenchymal lesions, mostly in young patients in which is preferable to preserve fertili- ty. Intraoperative ultrasonography represent an impor- tant tool for the precise identification and marking of the lesion, helping guarantee a radical excision as accurate as possible. REFERENCES 1. Albers P, Albrecht W, Algaba F, et al. EAU Guidelines on testicu- lar Cancer. 2. Giannarini G, Dieckmann KP, Albers P, et al. Organ-sparing sur- gery for adult testicular tumours: a systematic review of the litera- ture. Eur Urol. 2010; 57:780-90. Correspondence Riccardo Boschian, MD (Corresponding Author) rboschian@gmail.com Giovanni Liguori, MD gioliguori33@gmail.com Stefano Bucci, MD urostef@yahoo.it Michele Bertolotto, MD bertolot@univ.trieste.it Carlo Trombetta, MD trombcar@units.it Department of Urology, University of Trieste, Trieste, Italy Boschian-Intraoperative ultrasound_Stesura Seveso 09/01/17 10:47 Pagina 336