Bangladesh Journal of Pharmacology Research Article AlphaAlphaAlpha---glucosidase inhibitory effect glucosidase inhibitory effect glucosidase inhibitory effect and enzyme kinetics of coastal me-and enzyme kinetics of coastal me-and enzyme kinetics of coastal me- dicinal plants dicinal plants dicinal plants BJP Introduction About 2.5-7% of the world's populations diagnosed with leading endocrine disease diabetes mellitus which causes morbidity and mortality (Seghrouchni et al., 2002). By the act of pancreatic -amylase, starch has hydrolyzed and absorbed as glucose in the small intestine by -glucosidases, leads to hyperglycemic condition. Moreover, the use of several approaches, - glucosidase inhibitors is one of the alternative thera- peutic approaches. Dietary carbohydrates such as mal- tose and sucrose absorbed by different types of - glucosidases viz. maltase, sucrase, glucoamylase and isomaltase present in the intestine. Thus, inhibition of these enzymes can definitely decrease the postprandial hyperglycemia and could be a key tactic in the manage- ments of diabetes mellitus (Hirsh et al., 1997). The -glucosidase inhibitors have isolated from natural sources, such as plants, foodstuffs and microbes (Kumar et al., 2011). Coastal medicinal plants also engage in recreation to treat diabetes, particularly where most people have limited resources and do not have access to modern treatment (Ramanathan, 2000). The main interest of studies in to medicinal plants with hypoglycemic effect is to understand the metabolic activities of human intestinal tract. Citrullus colocynthis used for constipa- tion, edema, bacterial infections, cancer and diabetes. The aerial part of C. colocynthis showed antimicrobial and anti-inflammatory, anti-oxidant and local anesthe- tic effect reported by Gurudeeban et al., (2010). Suaeda monoica, S. maritime and S. nudiflora are traditional medicines for wound healing and hepatitis. The aerial part of this family plant has triterpenoids, sterols which have antiviral activity (Bandaranayake, 2002). Aegle A Journal of the Bangladesh Pharmacological Society (BDPS) Bangladesh J Pharmacol 2012; 7: 186-191 Journal homepage: www.banglajol.info; www.bdjpharmacol.com Abstracted/indexed in Academic Search Complete, Agroforestry Abstracts, Asia Journals Online, Bangladesh Journals Online, Biological Abstracts, BIOSIS Previews, CAB Abstracts, Current Abstracts, Directory of Open Access Journals, EMBASE/Excerpta Medica, Google Scholar, HINARI (WHO), International Pharmaceutical Abstracts, Open J-gate, Science Citation Index Expanded, SCOPUS and Social Sciences Citation Index ISSN: 1991-0088 Alpha-glucosidase inhibitory effect and enzyme kinetics of coastal medicinal plants Selvaraj Gurudeeban, Kaliyamurthi Satyavani and Thirugnanasambandam Ramanathan Marine Medicinal Plant Biotechnology Division, Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai 608502, Tamil Nadu, India. Abstract Numerous metabolic disorders and diseases associated with the glucosidase enzyme activity. Therefore, in the present study we evaluated alpha glucosidase inhibitory effect of coastal medicinal plants on Saccharomyces cerevisiae -glucosidase to control postprandial hyperglycemia. The n-hexane extracts of study plants screened for -glucosidase inhibitory activity using yeast glucosidase. The enzyme kinetics studied Line weaver burk plot method. Among the ten plants Citrullus colocynthis, Aegle marmelos and Ipomoea pes-caprae exhibited potent -glucosidase inhibitory activity, 85.9 ± 0.1, 72.2 ± 0.3 and 67.9 ± 0.1 respectively. From the enzyme kinetics assay potent plants contain uncompetitive inhibitors of alpha glucosidase. C. colocynthis, A. marmelos and Ipomoea pes-caprae have potential alpha- glucosidase inhibitors that can exploited for its used to treat diabetes. Article Info Received: 2 August 2012 Accepted: 3 September 2012 Available Online: 24 September 2012 DOI: 10.3329/bjp.v7i3.11499 Cite this article: Gurudeeban S, Satyavani K, Rama- nathan T. Alpha-glucosidase inhibito- ry effect and enzyme kinetics of coastal medicinal plants. Bangladesh J Pharmacol. 2012; 7: 186-91. This work is licensed under a Creative Commons Attribution 3.0 License. You are free to copy, distribute and perform the work. You must attribute the work in the manner specified by the author or licensor. marmelos, edible plant which are useful in ophthalmia, deafness, in ammations, catarrh, diabetes and asthmatic complaints, diarrhea, dysentery and stomachalgia, oph- thalmia, ulcers, dropsy, cholera and cardio tonic effect like digitalis (Nadkarni, 1976). Sesuvium portulacastrum has essential oils used as a remedy for fever and scurvy (Magwab et al., 2008). Clerodendrum inerme used to treat of skin diseases, veneral infections, elephantiasis and asthma (Anonymous, 2001). Among these conditions, 10 coastal medicinal plants used to manage diabetes in India namely C. colocynthis, Ipomoea pes-caprae, S. monoica, S. maritime, S. nudiflora, Sesuvium portulacastrum, A. marmelos, Clerodendrum inerme, Ruta graveolens and Casuarina equisetifolia. These medicinal plants are widely explored for their thera- peutic potential. However no reports are available on the mechanism of -glucosidase inhibitory effects. Therefore, the present study aimed to evaluate their effects on reduced glucose utilization effect. Materials and Methods Source of explants The aerial parts of the 10 medicinal plants collected from the Southeast coast of India and kept under shade net (50%) house environment. The specimen certified by the Herbaria of C. A. S. in Marine Biology, Annama- lai University and Botanical Survey of India (BSI) Coimbatore, Tamil Nadu. Preparation of extract The aerial parts of the plants extracted with n-hexane using a Soxhlet assembly for 48 hours, filtered and last traces of the solvent evaporated under reduced pre- ssure in a rotary evaporator. The resulting extract filtered through filter paper (Whatman No. 1). The experimental chemicals purchased from Sigma-Aldrich Mumbai. Assay for α-glucosidase inhibitory activity Alpha-glucosidase activity estimated according to the method previously reported by Shibano et al. (1997) with trivial alterations. 50 µL of 0.1M phosphate buffer (with pH of 7.0), 10 µL of test sample and 25 µL of - glucosidase solution incubated at 37ºC for 30 min and blanks prepared simultaneously. Then the reaction terminated by the addition of 100 µL of 0.2 M sodium carbonate solution. The enzymatic hydrolysis of subs- trate examine by the amount of p-nitrophenol released in the reaction mixture at 410 nm using microplate reader. Controls conduct in an identical manner repla- cing the plant extract with methanol and -D-Glucose used as positive control. All experiments carried out in triplicates. The inhibition percentage of -glucosidase assess by the following formula: -glucosidase% = 100X (control-sample)/controlwhereas, Control = test– blank; Sample = test – blank. Kinetics of α-glucosidase inhibitor To determine the plants extracts inhibition against S. cerevisiae -glucosidase, with increasing concentration of PNPG (4-nitrophenyl -D-glucopyranoside) used as substrate in the absence and presence of plant extracts at different concentrations. The data determined by Line weaver-Burk plot analysis of the data (Line Bangladesh J Pharmacol 2012; 7: 186-191 187 Table I α-Glucosidase inhibitory effect of coastal medicinal plants (Southeast coast of India) Plant name Voucher No. Family Plant parts used % of -glucosidase inhibition Aegle marmelos AUCASMB65 Rutaceae leaf & fruit 72.2 ± 0.3 Casuarina equisetifolia AUCASMB67 leaf 24.9 ± 0.4 Casuarinaceae Citrullus colocynthis AUCASMB66 whole part 85.9 ± 0.1 Clerodendrum inerme AUCASMB33 Verbenaceae leaf 41.0 ± 0.3 Ipomoea pes-caprae AUCASMB68 Convolvulaceae whole part 67.9 ± 0.1 Ruta graveolens AUCASMB69 Rutaceae leaf & fruit 25.8 ± 0.2 Suaeda monoica AUCASMB19 Chenopodiaceae whole part No activity Suaeda maritime AUCASMB20 Suaeda nudiflora AUCASMB21 Sesuvium portulacastrum AUCASMB22 Aizoaceae weaver, 1934), which calculated from the result according to Michaelis-Menten kinetics. Statistical analysis All assays performed at three times with triplicates samples. Values represented as mean ± Standard devia- tion. Results and Discussion Coastal plants are most promising source for recent drug development in marine pharmacological research. Earlier reports revealed the basic reason for diabetic mellitus, that the enzyme -glucosidase participated in biological process like digestion, biosynthesis of glycol- protein and mainly involve in the cleavage of glycosidic bond, as a result increase in blood glucose level leads to chronic hyperglycemia. One of the therapeutic approa- ches for decreasing postprandial hyperglycemia is to retard absorption of glucose by carbohydrate hydro- lyzing enzymes, -amylase and -glucosidase inhibi- tion, in the digestive organs (Deshpande et al., 2009). Hence, we evaluated 10 coastal plants to develop such eminent -glucosidase inhibitors from marine floral resources for reducing wide range of side effects. Among the 10 study plants A. marmelos, C. colocynthis and I. pes-caprae showed moderate -glucosidase inhibitory activity. Ruta graveolens and Casuarina equisetifolia showed less inhibitory activity and salt marsh plants S. monoica, S. maritime, S. nudiflora and Sesuvium portulacastrum did not posses any inhibition 188 Bangladesh J Pharmacol 2012; 7: 186-191 Table II Kinetics effect of α-glucosidase inhibition on plant extracts Citrullus colocynthis Aegle marmelos Ipomoea pes-caprae Concentration (µg/mol) Km (mM) Vmax(mM/min -1) Km (mM) Vmax (mM/min -1) Km (mM) Vmax(mM/min -1) 0 1.226 0.883 3.900 0.334 2.633 0.243 4 1.562 4.329 0.365 5.675 0.250 8 0.886 0.851 2.633 0.312 4.248 0.243 Figure 1 : Lineweaver-Burk plot of S. cerevisiae -glucosidase and PNPG with 4 µg/mL and 8 µg/mL of citrullus colocynthis extract Inhibitor concentration 4 µg Inhibitor concentration 8 µg Uninhibited reaction -0.3 -0.2 -0.1 0 0.1 0.2 0.3 0.4 0.5 60 50 40 30 20 10 0 -10 -20 1/ V (m M /m in ).1 1/S (mM) against S. cerevisiae -glucosidase. Acarbose used as positive control which showed low -glucosidase inhibitory potential with IC50 value of 133 μg/mL (Table I). Earlier studies, most mammalian -glucosidase inhibi- tors did not effectively inhibit microbial -glucosidases (Oki et al., 1999), because plants and mammalian enzymes are maltostructure, it hydrolyze homogeneous substrate whereas bacterial, yeast, and insect enzymes are maltooligosaccharides show higher activity toward heterogeneous substrates such as sucrose and pNPG, and no activity toward homogeneous substrates (Kimura, 2002; Kimura et al., 2004). Our present result exhibits the strong enzymatic inhibitory activity against yeast - glucosidases shown by three plant extracts (A. marmelos, C. colocynthis and I. pes-caprae) is obviously enhanced than the commercial inhibitor acarbose at low concentration. This will favor that A. marmelos, C. colocynthis and I. pes-caprae extracts can decrease blood glucose level. The inhibition kinetics of A. marmelos, C. colocynthis and I. pes-caprae analyzed by Lineweaver-Burk plot analysis, which indicated that it is a non-competitive inhibitor with respect to PNPG for S. cerevisiae -glucosidase (Table II). Inhibitor concentration (μg/mL) plotted on the X-axis and 1/V (mM/min)-1 values obtained from the Lineweaver-Burk plot plotted on the Y-axis. The aerial extract of C. colocynthis has uncompetitive inhibitor (s) of alpha-glucosidase (Figure 1) and reduc- tion of Vmax from 1.2 mM/min-1 to 0.8 mM/min-1 and Km from 1.6 mM to 0.9 mM. The leaf and fruit extract of A. marmelos has mixed inhibitors of alpha glucosidase (Figure 2) and there is a reduction in Vmax from 3.7 mM/min-1 to 3.1 mM/min-1 and Km from 4.3 mM to 2.6 mM. The aerial extract of I. pes-caprae (Figure 3) has uncompetitive inhibitors of alpha-glucosidase and there is reduction of Vmax from 0.3 mM/min-1 to 0.2 mM/ min-1 and the Km from 5.7 mM to 4.2 mM. The commercial alpha-glucosidase inhibitor, acarbose is a competitive inhibitor of the enzyme and it required at higher concentration to reduce the post-prandial glucose level. The uncompetitive inhibitors bind to the enzyme-substrate complex, lowering the Km and the maximum enzyme activity (Vmax). Conclusion Inhibition of -glucosidase is one of the therapeutic approaches for preventing post postprandial hypergly- cemia. Hence, the explore for -glucosidase inhibitors in coastal medicinal plants is imperative because these inhibitors could control the postprandial hyperglycemia of diabetic patients. Acknowledgements The authors are gratefully acknowledged to the Director and Bangladesh J Pharmacol 2012; 7: 186-191 189 Figure 2 : Lineweaver-Burk plot of S. cerevisiae -glucosidase and PNPG with 4 µg/mL and 8 µg/mL of Aegle marmelos extracts Inhibitor concentration 4 µg Inhibitor concentration 8 µg Uninhibited reaction -0.3 -0.2 -0.1 0 0.1 0.2 0.3 0.4 0.5 80 70 60 50 40 30 20 10 0 1/ V (m M /m in ).1 1/S (mM) Dean, Faculty of Marine Sciences and authorities of Annamalai University, India for providing all support during the study period. References Anonymous I. Wealth of India. National Institute of Science Communication and Council of Scientific and Industrial Research, New Delhi. 2001; 2: 67-68. Bandaranayake WM. Bioactivities, bioactive compounds and chemical constituents of mangrove plants. Wet Ecol Manag. 2002; 10: 421-452. Deshpande MC, Venkateswarlu V, Babu RK, Trivedi RK. 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Bangladesh J Pharmacol 2012; 7: 186-191 191 Author Info Thirugnanasambandam Ramanathan (Principal contact) e-mail: marinepharmacology@ gmail.com DatePrinted: This article was downloaded by you on: Dec 12, 2017