Bangladesh Journal of Pharmacology Research Article AntiAntiAnti---cancer activity of cancer activity of cancer activity of Ruellia Ruellia Ruellia squarrosa squarrosa squarrosa against human prostate against human prostate against human prostate cancer cell linecancer cell linecancer cell line BJP Introduction Ongoing research is being focused to seek out effective treatments for cancer.It is hoped that search for com- pounds having significant anti-cancer and cytotoxic activities in plants may contribute to finding effective anti-cancer therapeutics. Many plants have recently reported with anti-cancer activity like Aspergillus niger (Channabasava et al., 2014) and Morus nigra (Qadir et al., 2014). Ruellia squarrosa belonging to family Acanthaceaeis commonly known as “Water Bluebell” or “Neeli Daisy”. Ruellia species are traditionally used for the treatment of flu, fever and inflammation and scientifica- lly have been proved to be anti-pyretic, analgesic, and anti-oxidant (Chen et al. 2006). The objective of this study was to evaluate the anti- cancer activity of R. squarrosa extracts against human prostate cancer (PC3) cell line. Materials and Methods Preparation of extracts The aerial and root parts of R. squarrosa were collected from the surrounding of BZU, Multan. The plant was identified by Prof. Altaf Ahmed Dasti, Institute of Pure and Applied Biology, BZU, Multan. Voucher specimens No. Fl.C. 1-2 were deposited in the herbarium. The shade dried plant material (roots and aerial parts) of R. squarrosa were ground; then subjected for maceration with dichloromethane and methanol for three days successively. The both dichloromethane and methanol extracts were concentrated by using the rotary evaporator. Anti-cancer assay (MTT assay) Anti-cancer activity was recorded in 96-well microplates by MTT assay. Human prostate cancer cells (PC3) were cultured in DMEM (Dulbecco's Modified Eagle's Medium), along with 5% of FBS (fetal bovine serum), 100 IU/mL of penicillin and 100 µg/mL of streptomycin in 75 cm2 flasks, and kept in 5% CO2 incubator at 37oC. Exponentially growing cells were harvested, counted with hemocytometer and diluted with a particular medium. Cell culture with the concentration of 1 x 105 cells/mL was prepared and introduced (100 µL/well) into 96-well plates. After incubation, medium was removed and 200 µL of fresh medium was added with concentrations of compounds A Journal of the Bangladesh Pharmacological Society (BDPS) Bangladesh J Pharmacol 2015; 10: 97-99 Journal homepage: www.banglajol.info Abstracted/indexed in Academic Search Complete, Agroforestry Abstracts, Asia Journals Online, Bangladesh Journals Online, Biological Abstracts, BIOSIS Previews, CAB Abstracts, Current Abstracts, Directory of Open Access Journals, EMBASE/Excerpta Medica, Google Scholar, HINARI (WHO), International Pharmaceutical Abstracts, Open J-gate, Science Citation Index Expanded, SCOPUS and Social Sciences Citation Index ISSN: 1991-0088 Abstract Ruellia species are traditionally used for the treatment of flu, fever and inflammation. The aim of this study was to evaluate the anti-cancer activity of Ruellia squarrosa extracts against human prostate cancer (PC3) cell line. Dichloromethane and methanol extracts of aerial and root parts of the plant were made by maceration. Anti-cancer activity was estimated by MTT assay and percentage inhibition of cells was calculated. Results of MTT showed that 30 mg/ml of dichloromethane extract of root parts of the plant showed moderate anti-cancer activity (58% inhibition) against PC3 cell line with IC50 15.4 ± 0.3. It was concluded that R. squarrosa (root) possesses anti-cancer activity against human prostate cancer (PC3) cell line. This work is licensed under a Creative Commons Attribution 3.0 License. You are free to copy, distribute and perform the work. You must attribute the work in the manner specified by the author or licensor. Anti-cancer activity of Ruellia squarrosa against human prostate cancer cell line Muhammad Khurram Afzal, Muhammad Uzair and Bashir Ahmad Chaudhry Faculty of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan. Article Info Received: 14 December 2014 Accepted: 22 January 2015 Available Online: 30 January 2015 DOI: 10.3329/bjp.v10i1.21211 Cite this article: Afzal MK, Uzair M, Chaudhry BA. Anti-cancer activity of Ruellia squar- rosa against human prostate cancer cell line. Bangladesh J Pharmacol. 2015; 10: 97-99. (1-30 µM). After 48 hours, 200 µL MTT (0.5 mg/mL) was added to each well and incubated further for 4 hours. 100 µL of DMSO was added to each well. The extent of MTT reduction was calculated by measuring the absorbance at 570 nm, using a micro plate reader. The cytotoxicity was measured as concentration causing 50% growth inhibition (IC50) for PC3 cells. The percent inhibition was determined by using the follow- ing formula: Graph was plotted against concentrations to calculate IC50. Statistical analysis A logistic linear regression model was fit to the data using Microsoft Excel 2013 to calculate the IC50. The data obtained were expressed as mean ± SD. A value of p<0.05 was considered as significant. Results and Discussion Dichloromethane and methanol extracts of roots and aerial parts of R. squarrosa were evaluated for anti- cancer activity by MTT assay against human prostate cancer (PC3) cell line. The anti-cancer activity of extracts is given in Figure 1. The dichloromethane extract of root part of the plant showed moderate anti- cancer activity against PC3 cells line with IC50 15.4 ± 0.3 using doxorubicin as standard. In the previous studies, extracts of Convolvulus arvensis have shown cytotoxic effects against Colo 205 cells (Kaur and Kalia, 2012), Morus nigra against human cervical cancer cell line (HeLa) (Qadir et al., 2014), Bidens pilosa against HeLa and KB cell lines (Sundara- rajan et al. 2006) and Carthamus oxyacanthus against murine B16-F1 melanoma cells (Alesiani et al., 2010). We have evaluated anti-cancer activity of R. squarrosa against human prostate cancer cell lines. MTS analysis indicated the anti-proliferative activity of methanol and dicholoromethane extracts of Aerial parts as well as roots of the plant. For all the extracts, decline in cell proliferation was dose dependent. At a dose of 30 mg/ ml, there was a sharp decline in cell proliferation by dicholoromethane extract of roots of R. squarrosa. At this dose almost 42% of the total cells survived and 58% became dead. Ruellia species are rich in flavonoids (Lin et al. 2006). The anti-cancer activity of flavonoids has already been established (Kandaswami et al., 2005). Therefore, anti- cancer activity of R. squarrosa may be due to the flavonoids present in it. Acknowledgment The authors are grateful to the Pharmacy Department, Bahauddin Zakariya University Multan, Pakistan. 98 Bangladesh J Pharmacol 2015; 10: 97-99 % In hi bi tio n Concentration (mg/mL) % Inhibition 60 50 40 30 20 10 0 AM AD RD RM Figure 1: Anti-cancer activity of R. squarrosa (A: Aerial parts; R: Roots; M: Methanol extract; D: Dicholoromethane extract) References Alesiani D, Canini A, D’Abrosca B, DellaGreca M, Fiorentino A, Mastellone C, Pacifico S. Antioxidant and antiprolifera- tive activities of phytochemicals from Quince Cydonia vulgaris peels. Food Chem. 2010; 118: 199-207. Channabasava, Govindappa M. First report of anti-cancer agent, lapachol producing endophyte, Aspergillus niger of Tabebuia argentea and its in vitro cytotoxicity assays. Bangladesh J Pharmacol. 2014; 9: 129-39. Chen FA, Shieh P, Kuo D, Hsieh C. Evaluation of the anti- oxidant activity of Ruellia tuberosa. Food Chem. 2006; 94: 14- 15. Kandaswami C, Lee LT, Lee PP, Hwang JJ, Ke FC, Huang YT, Lee MT. The antitumor activities of flavonoids. In Vivo. 2005; 19: 895-909. Lin C, Huang Y, Cheng L, Sheu S, Chen C. Bioactive flavonoid from Ruellia tuberosa. J China Med. 2006; 17: 103-09. Kaur M, Kalia AN. Convolvulus arvensis: A useful weed. Int J Pharm Pharm Sci. 2012; 4: 38-40 Parkin DM, Bray F, Ferlay J, Pisani P. Global cancer statistics. CA Cancer J Clin. 2005, 55; 74-108. Qadir MI, Ali M, Ibrahim Z. Anticancer activity of Morus nigra leaves extract. Bangladesh J Pharmacol. 2014; 9: 496-97. Bangladesh J Pharmacol 2015; 10: 97-99 99 Author Info Muhammad Khurram Afzal (Principal contact) e-mail: khurramafzal28@yahoo.com Your feedback about this paper 1. Number of times you have read this paper 2. Quality of paper 3. Your comments Bangladesh J Pharmacol 2015 10 9799: Author Info Muhammad Khurram Afzal Principal contact email khurramafzal28yahoocom: DatePrinted: This article was downloaded by you on: Jun 12, 2017 Text2: Dropdown3: [Click] Dropdown4: [0]