Bangladesh Journal of Pharmacology Research Article In vitro In vitro In vitro inhibitory potential of inhibitory potential of inhibitory potential of methanolic extract of methanolic extract of methanolic extract of Celosia ar-Celosia ar-Celosia ar- genteagenteagentea var. var. var. cristatacristatacristata on tyrosinase, on tyrosinase, on tyrosinase, acetylcholinesterase and butyryl-acetylcholinesterase and butyryl-acetylcholinesterase and butyryl- cholinesterase enzymescholinesterase enzymescholinesterase enzymes BJP Introduction Tyrosinase is an important enzyme involved in production of melanin which causes skin problems (Briganti et al., 2003). It also causes neurotoxicity associated with Parkinson’s disease (Khan, 2007). While Alzheimer’s disease is a neurodegenerative disorder associated with loss of memory and cognition (Blennow et al., 2006). It is due to the inefficiency of cholinergic function in the brain stated by cholinergic hypothesis (Perry, 1986) so cholinomimetic drugs can improve it. Discovery of novel tyrosinase, acetylcholinesterase and butyrylcholinesterase enzyme inhibitors from medicinal plants may open new road to the era of pharmaco- logical research (Asanuma et al., 2003; Akhondzadeh et al., 2003; Wettstein, 2000). Celosia argentea var. cristata (L.) O. Kuntze (Family: Amaranthaceae) commonly known as cockscomb, kulgha and sardabi (Kritikar and Basu, 1987) distribu- ted worldwide including Pakistan (Prajapati and Pyrohit, 2003). Traditionally it is used to treat atherosclerosis (Wang et al., 2010), inflammation, diarrhea (Tova, 2004), dysentery (Shanmugam et al., 2011), abscess, cancer, pain and urinary tract infections. It is anti-tussive, expectorant, and hypotensive (Duke et al., 2002). It contains cochliophilin, isoflavone, crista- tein, phenethyl-alcohol, kaempferol, quercetin, β- sitosteorol, octadecenoic acid, stigmasterol, saponins, celosin A-D (Xiang et al., 2010). The plant possess hepatoprotective (Sun et al., 2011), anti-oxidant (Pyo et al., 2008) and antiviral activity (Begam et al., 2006). This study was conducted to evaluate the pharmacological potential of plant in the inhibition of three important enzymes i.e., tyrosinase, acetylcholine esterase and butrylcholine esterase to provide basis for its use in skin hyperpigmentation, Parkinson’s disease, Alzheimer’s disease and dementia. Materials and Methods Chemicals and drugs Mushroom tyrosinase (EC 1.14.1.8.1, 30 U), Electric eel AchEstrase (Type-VI-S, EC 3.1.1.7), horse serum A Journal of the Bangladesh Pharmacological Society (BDPS) Bangladesh J Pharmacol 2015; 10: 449-454 Journal homepage: www.banglajol.info Abstracted/indexed in Academic Search Complete, Agroforestry Abstracts, Asia Journals Online, Bangladesh Journals Online, Biological Abstracts, BIOSIS Previews, CAB Abstracts, Current Abstracts, Directory of Open Access Journals, EMBASE/Excerpta Medica, Google Scholar, HINARI (WHO), International Pharmaceutical Abstracts, Open J-gate, Science Citation Index Expanded, SCOPUS and Social Sciences Citation Index ISSN: 1991-0088 Abstract In the current study, methanol extract of Celosia argentea var. cristata was tested for its inhibitory potential against tyrosinase, acetylcholinesterase and butyryl-cholinesterase enzymes at the concentration of 0.5 mM by ELISA microtiter plate assays. A significant tyrosinase inhibitory activity (63.6%), acetylcholi-nesterase inhibitory activity (80.3%) and butyrylcholinesterse inhibitory activity (68.2%) was shown by crude methanolic extract of C. argentea var. cristata with respective IC50 values of 268.5 ± 0.2 µg/mL, 73.6 ± 0.1 µg/mL and 132.8 ± 0.9 µg/mL. The result of this study reveals the use of C. argentea var. cristata in skin hyperpigmentation, Parkinson’s disease and neurodegenerative disorders like Alzheimer’s disease and dementia. Article Info Received: 5 April 2015 Accepted: 8 May 2015 Available Online: 24 May 2015 DOI: 10.3329/bjp.v10i2.22880 Cite this article: Saqib F, Janbaz KH, Sherwani MK. In vitro inhibitory potential of methanol- ic extract of Celosia argentea var. cristata on tyrosinase, acetylcholinesterase and butyrylcholinesterase enzymes. Bang- ladesh J Pharmacol. 2015; 10: 449-54. This work is licensed under a Creative Commons Attribution 3.0 License. You are free to copy, distribute and perform the work. You must attribute the work in the manner specified by the author or licensor. In vitro inhibitory potential of methanolic extract of Celosia argentea var. cristata on tyrosinase, acetylcholinesterase and butyrylcholinesterase enzymes Fatima Saqib, Khalid Hussain Janbaz and Maryam Khan Sherwani Faculty of Pharmacy, Bahaudin Zakariya University, Multan, Pakistan. BChEstrase (EC 3.1.1.8), L-dopamine, acetylthiocholine iodide, butyrylthiocholine chloride and DTNB (5,5'- dithiobis-(2-nitrobenzoic acid)) were purchased from Sigma chemicals Co. St Louis, MO, USA. All other chemicals were of the analytical grade available from Merck/Fluka/Sigma. Collection and authentication of plant material Whole plant of C. argentea var. cristata was collected from botanical garden of Bahauddin Zakariya University Multan and identified with the kind cooperation of an expert taxonomist (Prof. Altaf Dasti) from the Institute of Pure and Applied Biology at Bahauddin Zakariya University Multan, Punjab, Pakistan. A sample voucher (STW-231) was submitted to the herbarium of Institute of Pure and Applied Biology at Bahauddin Zakariya University Multan, Punjab, Pakistan. Extraction of crude extract The plant material was shade-dried and rendered free from soil and adulterated material and coarsely ground by electrical device. The powdered material was soaked into aqueous-ethanol (80%) for 72 hours with occasional shaking. The soaked material was rendered free from plant debris by passing through a muslin cloth and fluid portion was filtered through a fine filter paper (Williamson et al., 1998). The above mentioned extraction procedure was repeated twice on the plant debris and filtrate was subsequently combined before subjecting to evaporation under reduced pressure on a rotary evaporator to thick paste like mass of dark brown color, i.e., crude extract of C. argentea var. cristata, yielding approximately 11.1% (w/w). Preliminary phytochemical analysis Qualitative phytochemical analysis of crude extract was done for the pre-sence of alkaloids, anthraquinones, coumarins, saponins, flavonoids and tannins as reported elsewhere (Janbaz and Saqib, 2015) Tyrosinase inhibition activity Total volume of reaction mixture 100 μL containing 60 μL phosphate buffer (100 mM), pH 6.8, 10 μL mushroom tyrosinase enzyme (5 units) and 10 μL 0.5 mM test compound mixed in 96-well plate (Lee et al., 2009). Contents were pre incubated for 5 min at 37°C. After incubation, 20 μL of 10 mM L-dopamine was added as a substrate. Contents were mixed and incubated for further 30 min. Absorbance was taken at 490 nm using Synergy HT Biotek 96-well plate reader. The enzyme inhibition (%) was calculated by the bellow formula. %Inhibition = 100 - (absorbance of test sample/ absorbance of control × 100) Acetylcholinesterase inhibition activity The AChE inhibition activity was performed according to the method (Ellman et al., 1961) with slight modifications. Total volume of the reaction mixture was 100 µL. It contained 60 µL Na2HPO4 buffer with concentration of 50 mM and pH 7.7. Ten microliter test compound (0.5 mM/well) was added, followed by the addition of 10 µL (0.005 unit per well) enzyme. The contents were mixed and pre-read at 405 nm. Then contents were pre-incubated for 10 min at 37ºC. The reaction was initiated by the addition of 10 µL of 0.5 mM/well substrate (acetylthiocholine iodide), followed by the addition of 10 µL DTNB, 0.5 mM/well. After 30 min of incubation at 37ºC absorbance was measured at 405 nm using 96-well plate reader Synergy HT, biotek, USA. All experiments were carried out with their respective controls in triplicate. Eserine (0.5 mM/well) was used as a positive control. The percent inhibition was calculated by the help of following equation. Control – Test Inhibition (%) = X 100 Control Butyrylcholinesterase inhibition activity The BChE inhibition activity was performed according to the method (Ellman et al., 1961) with slight modifications. Total volume of the reaction mixture was 100 µL containing 60 µL, Na2H PO4 buffer, 50 mM and pH 7.7. The 10 µL test compound 0.5 mM per well was added followed by the addition of 10 µL (0.5 unit per well) BChE (Sigma Inc.). The contents were mixed and pre-read at 405 nm and then pre-incubated for 10 min at 37ºC. The reaction was initiated by the addition of 10 µL of 0.5 mM/well substrate (butyrylthiocholine chloride). Followed by the addition of 10 µL DTNB (5,5'-dithiobis- (2-nitrobenzoic acid)), 0.5 mM well-1. After 30 min of incubation at 37ºC, absorbance was measured at 405 nm using 96-well plate reader Synergy HT, Biotek, USA. All experiments were carried out with their respective controls in triplicate. Eserine (0.5 mM/well) was used as positive control. The percent inhibition was calculated by the help of following equation. Control – Test Inhibition (%) = X 100 Control Statistical analysis In all of the enzyme inhibition activities, the experi- ments were performed three times and the results were expressed as mean ± standard error of mean (S.E.M) of three parallel measurements and the respective. IC50 values were calculated using EZ–Fit Enzyme kinetics software (Perrella Scientific Inc. Amherst, USA). Amherst USA software. The data was analyzed by an analysis of variance p<0.05. Results were processed by computer programs: Excel and Ezi Fit software. 450 Bangladesh J Pharmacol 2015; 10: 449-454 Results The result of preliminary phytochemical analysis show- ed that extract of C. argentea var. cristata contains alka- loids, saponins, flavonoids, tannins and phenolic com- pounds. Very moderate antityrosinase activity was seen with the methanolic extract of Celosia argentea var. cristata at the dose of 0.5 mg/mL which showed 63.6% inhibition of tyrosinase with 50% inhibition (IC50) at a concentration of 268.9 ± 0.2 µg/mL (p<0.05) (Table I). The standard drug kojic acid, showed significant tyrosinase inhibition activity with the 0.5 mg/mL concentration showing a 93.5% inhibition of tyrosinase (IC50 6.0 ± 0.1 µg/mL). The %tyrosinase inhibition activity of crude extract and kojic acid are shown in a Figure 1. The 0.5 mg/mL of methanolic extract of C. argentea var. cristata showed 80.3% inhibition of acetylcholinesterase and 68.2% inhibition of butyrylcholinesterase with respective 50% inhibition of acetylcholinesterase (IC50) at a concentration of 73.6 ± 0.1 µg/mL (p<0.01) and butyrylcholinesterase (IC50) at 132.8 ± 0.9 µg/mL (Table I). The standard drug eserine, showed significant 91.3% acetylcholinestersae and 82.8% butyrylcholinesterase inhibition activity with the 0.5 mg/mL concentration. The IC50 values of the extract was found to be greater than the standard, eserine which showed an IC50 of acetylcholinesterase at concentration of 0.04 ± 0.0 µg/ mL and IC50 of butyrylcholinesterase at 0.9 ± 0.0 µg/mL. The %acetylcholinesterase and butyrylcholines-terase inhibition activity of crude extract and standard, eserine are shown in a Figure 2. Discussion The skin is an important barrier that protects our body from damage due to its direct contact with the outside environment. Melanin is the important pigment in the skin. It protects our skin from UV damage by absorbing sunlight and removing reactive oxygen species. About Bangladesh J Pharmacol 2015; 10: 449-454 451 Table I Inhibitory effects of crude extract of Celosia argentea var. cristata on tyrosinase, acetylcholinesterase and bu- tyrylcholinesterase Sample Conc. (mM) %Tyrosinase inhibition %AChE inhibition %BChE inhibition IC50 (µmol) Tyrosinase AChE BChE Crude extract 0.5 mM 63.6 ± 0.6 80.3 ± 0.8 68.2 ± 0.7 268.9 ± 0.2 73.6 ± 0.1 132.8 ± 0.9 Eserine 0.5 mM - 91.3 ± 1.2 82.8 ± 1.1 - 0.0 ± 0.0 0.9 ± 0.0 Kojic acid 0.5 mM 93.5 ± 0.9 - - 6.0 ± 0.1 - - All experiments are performed in triplicate and represented as mean± SEM; Standard drugs used are Kojic acid for tyrosinase inhibition and eserine for AChE and BChE inhibition K ojic A ci d(0 .5 m M ) Cc.C r(0 .5 m M ) 0 25 50 75 100 % In hi bi tio n of ty ro si na se Kojic acid (0.5 mM) Cc.Cr (0.5 mM) 100 75 50 25 0 Figure 1: Showing percentage inhibition of tyrosinase by crude extract of Celosia argentea var. cristata (Cc.cr) and standard drug kojic acid Figure 2: Showing percentage inhibition of acetyl cholinester- ase inhibition (AChE) and butyryl cholinesterase (BChE) by crude extract of Celosia argentea var. cristata (Cc.Cr) and stand- ard drug eserine P er ce nt ag e in hi bi tio n A C h E B C h E 0 20 40 60 80 100 Enzyme 100 80 60 40 20 0 AChE BChE Cc.Cr (0.5 mM) Eserine (0.5 mM) Cc E 10% of skin cells in the innermost layer of epidermis produce melanin. Upon exposure to UV radiation, melanogenesis is initiated by the enzyme tyrosinase resulting in skin darkening (Vamos, 1981). As discussed earlier, tyrosinase is involved in the transformation of tyrosine to o-dopaquinone. The hyperpigmented skin is observed in various dermatological disorders namely melasoma, solar lentigines and ephelides (Maeda and Fukuda, 1991). The reactive oxygen species produced in the human body enhances the DNA damage, melanin biosynthesis and induces the proliferation of melano- cytes (Yasui and Sakurai, 2003) which results in hyper pigmentation disorders. The production of melanin in the body can be reduced by several mechanisms inclu- ding direct inhibition of tyrosinase enzyme, inhibiting the transport of melanosome to the stratum corneum, the supplementation of anti-oxidants (Pawelek and Kormer, 1982) as well. The most common skin lightening and depigmentation agents available commercially are kojic acid, arbutin, catechins, hydroquinone (HQ) and azelaic acid (Maeda and Fukuda, 1996). Some adverse effects of these syn- thetic compounds are irreversible cutaneous damage, ochronosis etc. These adverse effects have led to the search for safer plant-based skin lightening ingredients. The aqueous methanolic extract of C. argentea var. cristata showed a moderate anti-tyrosinase activity with 63.6% inhibition. The preliminary chemical examination of the crude extract of C. argentea var. cristata has demonstrated the presence of phenolic and flavonoid compounds which may contribute to its tyrosinase inhibitory effect as reported earlier (Kubo et al., 2003; Nagendra et al., 2009). Anti-oxidant activity also affect tyrosinase activity (Kim et al., 2008). The potent skin whitening and anti-oxidant ability of C. argentea var. cristata make it a suitable candidate for the remedy of hyper pigmentation disorders of skin. AChE (EC 3.1.1.7) comprise a family of enzymes which include serine hydrolases. They share about 55% of amino acid sequence identity and have similar catalytic properties. The different specificities for substrates and inhibitors are due to the difference in amino acid residues of the active sites of AChE and BChE (Cyglar et al., 1993). Acetylcholinesterase plays a prominent role as it participate in termination of acetylcholine based signal transmission through neurosynaptic cleft or neuromuscular junction (Ballard et al., 2005; Khaled et al., 2010). Butyrylcholinesterase (BChE) is a non-specific ester hydrolyzing enzyme with no known endogenous physiological substrate (Quinn, 1987). It can hydrolyze various esters of choline and other compounds (Sun et al., 2001), e.g., cocaine, some organophosphorus compounds. BChE is produced in liver and enriched in blood circulation. In addition, it is also present in adipose tissues, intestine, smooth muscle cells, and white matter of the brain (Schwarz et al., 1995). H1 and H2 receptor antagonists have been shown to possess AChE inhibitory activities. A deficit of ACh levels in CNS leads to conditions such as Alzheimer’s disease (AD) which is the most common form of dementia, a progressive neurologic disease of the brain that leads to loss of mental ability severe enough to interfere with normal activities of daily living and decline in cognitive functions such as remembering, reasoning and planning. It affects parts of the brain that control thought, memory, and language. It is characterized by nerve-cell loss, abnormal tangles and plaques within nerve cells and deficiencies of several neurochemicals such as acetylcholine (ACh) and butyrylcholine (BCh), which are essential for the transmission of nerve messages. It was postulated that blocking the enzyme cholinesterase (ChE) induced hydrolysis of ACh and subsequent increase in Ach concentration in central synapses and enhancement of cholinergic functions provides the symptomatic improvement to AD patient (Schwarz et al., 1995; Pohanka et al., 2009). ChE inhibitors were developed to improve the effectiveness of ACh by inhibiting its breakdown and increasing the levels in the brain or by strengthening the way nerve cells responds to it. Increased concentrations of ACh in the brain leads to increased communication between nerve cells and may temporarily improve or stabilize the symptoms of AD. These drugs appear to work best in the early and moderate stages of AD. It has been further suggested that dual inhibition of AChE and BChE enzymes should be one of the objectives in the treatment of cognitive dysfunction associated with AD (Pohanka et al., 2010). The results obtained with the crude extract of C. argentea var. cristata, exhibits outstanding enzyme inhibitory activity against AChE (80.3%), and BChE (68.2%). The outstanding results obtained with the crude extracts of C. argentea var. cristata, indicate the need for further work on the isolation, purification and investigation of the active principles responsible for the extract inhibitory activity. Conclusion The crude extract of Celosia argentea var. cristata possess- es inhibitory activity against tyrosinase, acetylcholine estrase and butrylcholineestrase enzymes which pro- vide a base for its use in hyperpigmentation of skin, Parkinson’s disease and other neurodegenerative disorders like Alzheimer’s disease and dementia. Competing Interest The authors declare that they have no competing interests. 452 Bangladesh J Pharmacol 2015; 10: 449-454 Acknowledgment The authors acknowledge Bahauddin Zakariya University and Islamia University for providing research facilities. 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J Jilin Agric Univ. 2010; 32: 657-60. Yasui H, Sakurai H. Age dependent generation of reactive oxygen species in the skin of live hairless rats exposed to UVA light. Exp Dermatol. 2003; 12: 655-61. 454 Bangladesh J Pharmacol 2015; 10: 449-454 Author Info Fatima Saqib (Principal contact) e-mail: fatima2saqib@yahoo.com mailto:fatima2saqib@yahoo.com