Introduction Overview of exopolysaccharide Lactic acid bacteria are widely exploited in medicine, and traditional dairy products, as well as in biotechno- logical and industrial fermentation processes as a well- established starter culture (Park, 2001; Savadogo et al., 2004; Park et al., 2008; Kim et al., 2013a; Rather et al., 2013; Park et al., 2014). Lactic acid bacteria have shown a significant importance in health complications with increasing number of health beneficial microflora in the intestinal tract (Park et al., 2010), along with an ability to synthesize functional exopolysaccharides (Ricciardi and Clementi 2000; Seo et al., 2001; Savadogo et al., 2004; Kim et al., 2006; Kim et al., 2007). In addition, an important role of lactic acid bacteria has been noticed in the food fermentation, since lactic acid bacteria-derived fermented foods display increased rate of hygienic safety, storage stability and attractive sensory proper- ties (Rather et al., 2014). Traditional differentiation of lactic acid bacteria species can be accomplished through their identification and detection by employing various A Journal of the Bangladesh Pharmacological Society (BDPS) Bangladesh J Pharmacol 2016; 11: 1-23 Journal homepage: www.banglajol.info Abstracted/indexed in Academic Search Complete, Agroforestry Abstracts, Asia Journals Online, Bangladesh Journals Online, Biological Abstracts, BIOSIS Previews, CAB Abstracts, Current Abstracts, Directory of Open Access Journals, EMBASE/Excerpta Medica, Google Scholar, HINARI (WHO), International Pharmaceutical Abstracts, Open J-gate, Science Citation Index Expanded, SCOPUS and Social Sciences Citation Index; ISSN: 1991-0088 Abstract Lactic acid bacteria exhibit the most effective potential to divert significant amount of fermentable sugars towards the biosynthesis of functional exopolysaccharide. Exopolysaccharides from lactic acid bacteria are receiving a renewed interest due to the claims of human health benefits. This review provides an update on multiple uses and production of exopolysaccharides with major emphasis on their chemical properties, characterization, and some other molecular strategies adopted for their genetics and biological tailoring to better understand the process of exopolysaccharide production along with their antiviral efficacy with multiple modes of action. Additionally, microbiological, biochemical, nutritional and biotechnological aspects of exopolysaccharide production have also been discussed. Moreover, appro- priate suggestions have been made on lactic acid bacteria improvements, leading to enhanced production with advanced modification and production process that may contribute to the economic soundness of applications in food and pharmacological industries with this promising group of bio- molecules. Article Info Received: 22 June 2015 Accepted: 9 July 2015 Available Online: 16 December 2015 DOI: 10.3329/bjp.v11i1.23819 Cite this article: Bajpai VK, Rather IA, Majumder R, Shukla S, Aeron A, Kim K, Kang SC, Dubey RC, Maheshwari DK, Lim J, Park YH. Exopolysaccharide and lactic acid bacteria: Perception, func- tionality and prospects. Bangladesh J Pharmacol. 2016; 11: 1-23. Exopolysaccharide and lactic acid bacteria: Perception, functionality and prospects Vivek K. Bajpai1, Irfan Ahmad Rather1, Rajib Majumder1, Shruti Shukla2, Abhinav Aeron3, Kangmin Kim3, Sun-Chul Kang4, R. C. Dubey5, D. K. Maheshwari5, Jeongheui Lim6 and Yong-Ha Park1 1Department of Applied Microbiology and Biotechnology, School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Korea; 2Department of Food Science and Technology, Yeungnam University, 280 Daehak-ro, Gyeongsan-si, Gyeongsangbuk-do 712-749, Korea; 3Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, 79 Gobong-ro, Iksan-si 570-752, Joellabuk- do (Jeonbuk), Korea; 4Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk, 712-714, Korea; 5Department of Botany and Microbiology, Faculty of Life Sciences, Gurukul Kangri University, Haridwar 249-404, Uttarakhand, India; 6National Science Museum, Yuseong-gu, Daejeon, 305-705, Korea. M in i-r ev ie w http://www.bioxbio.com/if/html/BANGL-J-PHARMACOL.html http://www.asiajol.info/index.php/record/view/49691 molecular methods as potential alternatives in order to assess their quality control measures in dairy products (Bae et al., 2003; Kim et al., 2003; Savadogo et al., 2004; Koh et al., 2004; Bae et al., 2005). Due to the versatile potentialily of microbial exopoly- saccharides to work as a texturizer, viscosifer, emul- sifier and syneresis-lowering agent, as well as due to their pseudo-plastic rheological behavior and water binding capacity, they have shown demanding Indus- trial need especially in food industry (Kodali et al., 2009). A wide range of different lactic acid bacteria produce different types of chemically-structured forms of exopolysaccharides. Since, there is no confirmed reports available on the harmful effects of lactic acid bacteria so far, they are classified as Generally Regarded as Safe (GRAS) microorganisms (Yadav et al., 2011). The microbial exopolysaccharides play a vital role to conceal the bacterial surface facilitating an adhesive interaction at the surface of other bacteria (Yadav et al., 2011). Moreover, they also work as a substance in the rhizosphere community for bacterial aggregation as well as environment protective agents (Badel et al., 2011). Since exopolysaccharides significant -ly contribute to the specific rheology and smooth textural properties of fermented and milk products, they have become major targets of ongoing research especially in food processing industry (Frengova et al., 2002; Habibi et al., 2011). Production of exopolysaccha- ridesis considered a unique feature of lactic acid bacteria in the formation of starters for fermented milk products. In addition, EPSs have shown number of health beneficial effects in human beings especially in the treatment of gastrointestinal, tumor and bowl diseases (Gibson and Rastall, 2003). Lactic acid bacteria-derived exopolysaccharides, altho- ugh produced in a very less amount in fermented yogurt, play a crucial role in improved smooth and creamy texture of yogurt, one of the very important aspects of yogurt quality. These EPSs have also shown industrial effectiveness in the development of improved quality low-milk-solid yogurt, low-fat yogurt, and cream yogurt with various health beneficial properties (Feldmane et al., 2013). In addition, various health beneficial attributes of lactic acid bacteria-derived exopolysaccharides have been confirmed previously either as non-digestible food fractions (Patel and Prajapati, 2013) or being natural candidates to treat cancer, ulcer and immune modulation along with their potent ability to reduce blood cholesterol levels (Madhuri and Prabhakar, 2014). Lactic acid bacteria and lactic acid bacteria-derived exopolysaccharides also have significant economic and therapeutic potential for the development of nutrient rich functional food products with prolonged human health beneficial effects. Interestingly, exopolysaccharides may also play an important role by interacting with human immune system serving as vital component of functional foods as well as provide healing effects in bowel disease by working as prebiotics (Vinderola et al., 2006; Bello et al., 2001). A few selected lactic acid bacteria display exopolysaccharide production in the form of glucans or fructans by utilizing sucrose as sole carbon source through the action of glycosyl-transferase enzymes (Tieking et al., 2005). In addition, although, exopoly- saccharides show potential ability to colonize dental surfaces by Streptococci, non-significant importance has been given on the relevance to the ecological niche of gastrointestinal biome lactic acid bacteria (Banas and Vickerman, 2003; Sims et al., 2011) Lactic acid bacteria-derived exopolysaccharides in composition might exist as a single type of sugar monomer (homo-polysaccharides) or in the combina- tion of several types of monomers (hetero-polysaccha- rides). However, variations in sugar composition, chain length, degree of branching, or sugar linkages in the exopolysaccharides produced by different lactic acid bacteria have been observed as leading factors, which assist in the termination of the rheological and health- promoting potential of lactic acid bacteria-derived exopolysaccharides (Ruas-Madiedo et al., 2002). Based on the chemical composition of lactic acid bacteria- derived exopolysaccharides, they have been classified in two chemical classes, homo-exopolysaccharide and hetero-exopolysaccharide (Harutoshi, 2013). Homo- exopolysaccharides are the chemical structures of single type of monosaccharide, whereas, hetero-exopolysa- ccharides contain regular repeating units of 3-8 different carbohydrate moieties synthesized from intracellular sugar nucleotide precursors (Ganzle et al., 2005). However, biosynthesis process of both homo- and hetero-exopolysaccharides differs from each other. Synthesis of homo-exopolysaccharide occurs through the enzymatic reactions of glucansucrase or levan- sucrase by using sucrose (van Hijum et al., 2004), whereas, hetero-exopolysaccharide synthesis completes in four major steps involving sugar transportation, sugar nucleotide synthesis, repeating unit synthesis, and polymerization of the repeating units (de Vuyst and Degeest, 1999). Generally exopolysaccharides are produced either in a bioreactor or in situ through proper down-stream processing for their further practical applications as a functional food additives and in fermentation purposes. Since lactic acid bacteria are often used in the preparation of fermented mixed starter cultures for dairy fermented food products, application of exopoly- saccharides as bio-ingredients in food industry depends on the recovery rate and economic yield production (Sanchez et al., 2006). Lactic acid bacteria are the 2 Bangladesh J Pharmacol 2016; 11: 1-23 predominant microbiota and play an important role in natural fermentation of meat and vegetables and are used as mixed starters in controlled fermentation process (Sanchez et al., 2006). In addition, mesophilic lactobacilli bacteria as a secondary microbiota have also shown significant role in the development of unique flavor and texture of cheese products (Wouters et al., 2002). Biotechnological advances have led to the discovery of lactic acid bacteria-derived biopolymers or exopoly- saccharide molecules with confirmed evidences of industrial and medical usefulness to mankind. Enriched with biocompatibility, being GRAS and non-toxic ability of lactic acid bacteria-derived exopolysaccha- rides have made them a first-line choice in the treat- ment of various chronic diseases including tissue engi- neering, drug delivery system, and disease healing ability as compared to the plants and algal-based polysaccharides (Otero and Vincenzini, 2003). Reports have confirmed that a few selected biopolymers can be degraded in vivo, they might be possible alternatives to synthetic biopolymers for using in tissue replacement and controlled drug release strategies (Rehm, 2010). This overview provides recent advancements on the knowledge of functional properties of lactic acid bacteria-derived classified exopolysaccharides, their chemical nature, molecular characterization, genetic synthesis and applications in medical and industrial sector with specially emphasis on their future prospects. Classification and chemical composition of exopoly- saccharide The microbial exopolysaccharide from lactic acid bacteria can be divided into two major groups such as homo- exopolysaccharides and hetero-exopolysaccharides. An overview on the classification of lactic acid bacteria- derived exopolysaccharides has been summarized in Figure 1. In microbial cells, hetero- and some homo- polysaccharides are synthesized and secreted into the extracellular environment. Although synthesis of homo- polysaccharide occurs outside the cells after specific enzymes are exuded (Donota et al., 2012). Homo- exopolysaccharides consist of four sub-groups including -D-glucans, -D-glucans, fructans and poly-galactans (Harutoshi, 2013). The homo-exopolysaccharides, -D- glucans such as dextrans produced from Leuconostoc mesenteroides subsp. mesenteroides and L. mesenteroides subsp. dextranicum, alternans from L. mesenteroides and mutans from Streptococcus mutants and S. sobrinus are mainly composed of -1,6 and -1,3-linked glucose molecules with variable degrees of branching at position 3, although, they may also be present at position 2 or 4 however less frequently (de Vuyst and Degeest, 1999; Harutoshi, 2013). On the other hand, homo- exopolysaccharides such as -D-glucans produced by Pediococcus and Streptococcus species are composed of glucose molecules linked to -1,3 residual position along with -1,2 branching. Mono-polysaccharides, fructans produced from Streptococcus salivarius are linked to -fructose molecules at -2,6 residual position along with -2,1-branching on O1 site. The poly- galactans types of homo-exopolysaccharides are composed of repeating sugar units which are identical in their chemical structures and linked together with different glycosidic bonds. The hetero-exopoly- saccharides are produced by the mesophilic and thermophilic lactic acid bacteria. The major mesophilic lactic acid bacteria include L. lactis sub-sp. lactis, L. rhamnosus, L. lactis sub-sp. cremoris, L. sakei, L. casei whereas L. delbrueckii sub-sp. bulgaricus, L. acidophilus, L. helveticus and S. thermophiles which are considered to be the major representatives of thermophlic lactic acid bacteria. Sugar linkage pattern of some of the selected lactic acid bacteria-derived exopolysaccharides has been given in Figure 2. Homo-exopolysaccharide The lactic acid bacteria have ability to produce exopolysaccharide either as an environmental secretion or cell adhered EPS in a capsular form (Harutoshi, 2013). Generally exopolysaccharides are classified into two major groups including homo-exopolysaccharide a n d h e t e r o - e x o p o l y s a c c h a r i d e . H o m o - exopolysaccharides are consisting of single type of monosaccharide such as -D-glucans, -D-glucans, fructans, and polygalactans, whereas, hetero- exopolysaccharides are formed of different types of monosaccharides including D-glucose, D-galactose, L- rhamnose, and their derivatives (Mayo et al., 2010; Harutoshi, 2013). Homo-exopolysaccharides possess differences with e→ch other’s due to v→rious import→nt factors such as the primary structural skeleton including pattern of chain bonds, brand structures and molecular weights. The most significant groups of lactic acid bacteria-derived exopolysaccharide are represen- ted by -glucans such as dextrans and mutans which are produced by L. mesenteroides and S. mutans, respectively; whereas fructans such as levan are produced by S. salivarius (Cerning, 1990). Dextran is generally produced from sucrose in L. mesenteroides sub-sp. Mesenteroides (Cerning, 1990). However, increased rate of salt components has shown negative effect on dextran formation in the growth medium during serial transformation. Moreover, Leuconostoc species such as L. amelibiosum can also produce dextran in the growth medium when supplemented with orange or tomato juice (Vuyst and Degeest, 1999). Generally the members of dextran family share -1,6- linkages with variable degree of branching at residual position of 2, 3 or 4 (Harutoshi, 2013). A number of strains of Streptococcus species have shown the ability to form mutan, a homo exopolysaccharide glucan with -1,3 linkages which differs from dextran Bangladesh J Pharmacol 2016; 11: 1-23 3 F ig u re 1 : C la ss if ic a ti o n o f b a ct er ia l ex o p o ly sa cc h a ri d e 4 Bangladesh J Pharmacol 2016; 11: 1-23 Figure 2: Sugar linkage pattern of various EPSs isolated form different lactic acid bacteria (LAB) [Gruter et al., 1993; Landersjo et al., 2001; Robijin et al., 1995; van Casteren et al., 2000; van Casteren, 2001; Yamamoto et al., 1995] Bangladesh J Pharmacol 2016; 11: 1-23 5 being highly soluble in water as well as dextran possesses higher number of -1,6 linkages (Cerning, 1990). Since water insoluble mutan helps to facilitate microorganisms to attach on teeth surface, it is highly consumed in dental cavities and dental caries. Another glucan homo-exopolysaccharide, an altrnan firstly isolated from L. mesenteroides NRRL B-1355 with distinctive physical attributes possesses -1,6 and -1,3 alternate linkage, making it to be a component of high water solubility and low viscosity potential for using at industrial purposes, especially in food industry as a low viscosity texturizer (Cote and Robyt, 1982; Harutoshi, 2013). A fructan homo-exopolysaccharide, levan is produced in the growth medium utilizing sucrose as carbon source, which is composed of -2,6-linked fructose units with -2,1-linked branching (Harutoshi, 2013). For example, a fructan, insulin is also composed of -2,1- linked fructose sugar units along with -2,6-linked branching. Previously S. salivarius, L. mesenteroides, and L. reuteri have shown the ability to synthesize homo- EPS levan (Uchida, 1996). A homo-exopolysaccharide, fructan has also been reported to produce from L. sanfranciscensis (Korakli et al.,2002). Chemical struc- tures of some of the beneficial and industrial homo- exopolysaccharides have been provided in Figure 3A. Hetero-exopolysaccharide The biopolymer members of hetero-exopolysaccharide show diverse range of chemical differentiation among e→ch other’s, composed of repe→ting units of m→inly D- glucose, D-galactose, and L-rhamnose (Harutoshi, 2013). The chemical composition and repeating struc- tural subunits of monosaccharides used to form a hetero-EPS polymer do not mean to confer species specificity. Although exceptions exist such as a Lactobacillus species, L. kefiranofaciens subsp. Kefirano- faciens, isolated from a fermented food product Kefir in North Caucasus, were able to produce enormous amount of hetero-exopolysaccharides (De Vos et al., 2009; Harutoshi, 2013). A number of microbial species such as Streptococcus thermophilus, Lactococcus lactis, L. delbrueckii, and Lactobacillus helveticus have been well recognized with an ability to produce hetero- exopolysaccharides (Mozzi et al., 2006) In addition, lactic acid bacteria of homo-fermentative nature are well known for their ability to produce hetero-EPS, whereas, a hetero-fermentative strain L. fermentum has been found to produce exopolysaccharide with confirmed chemical nature determination (Leo et al., 2007). Although previously presence of inadequate production of hetero-exopolysaccharides has been confirmed in L. brevis and L. buchneri in the growth medium containing glucose or sucrose as carbon sources, no results were reported on the origin (Figueroa et al., 1995). In general, the lactic acid bacteria have produced a wide variety of hetero-exopolysaccharides, however they have shown wide range of variations. A few bacterial isolates such as S. thermophiles, L. lactis sub-sp. cremoris, L. delbrueckii sub-sp. bulgaricus, L. casei and L. plantarum found to produce 50-350, 80-600, 60-150, 50-60 and about 140 mg/liter of hetero-exopolysaccharide (Tallon et al., 2003; Tsuda and Miyamoto, 2010; Harutoshi, 2013). The maximum amount of exopolysaccharide recovery has been noticed for L. rhamnosus RW-9595 and L. kefiranofaciens WT-2B by 2,275 and 2,500 mg/liter, respectively (Maeda et al., 2004). Lactic acid bacteria have shown less ability on exopolysaccharide production than the microorganisms of industrial importance such as Xanthomonas campestris with about xanthan exopolysaccharide production of 30 -50 g/liter (de Vuyst and Degeest, 1999; Harutoshi, 2013). However, in situ exploitation has been success- fully achieved for exopolysaccharides production from lactic acid bacteria, making them to be the candidates of choice for enhanced production of exopolysaccharides with higher yields, and their practical applications in food, pharma and dairy industries. Chemical structures of some of the beneficial hetero-exopolysaccharides have been given in Figure 3B. Molecular characterization of exopolysaccharide Molecular characterization of exopolysaccharides requi- res a complete information of molecular mass and identification of absolute configuration and composi- tion of monomers leading to a final characterization of any polysaccharide molecule along with a linkage monomer pattern. Now-a-days, several analytical and spectral techniques are available to determine the molecular mass of any polysaccharide molecule. Previously size exclusion chromatography based on refractive index and retention time has been successfully used to determine the molecular mass of various polysaccharide molecules (Nichols et al., 2005). According to Williams et al., (1992), polysaccharide compounds are eluted very close to the limit of stationary phase during exclusion and represent very high molecular mass moieties. Application of combination detector which uses light scattering device to measure the molecular mass, can be able to determine molecular mass of polysaccharide molecules with significant accuracy (Law et al., 2001). In addition, application of various chromatographic methods have provided trustworthy results on the determination of chemical composition of polysaccharide monomers, among them methanolysis and per-trimethylsilylation have proved to be the significant steps for the processing of polysaccharide monomer samples which can be analyzed either by gas-chromatography and mass spectrometry or gas-liquid chromatography. Besides, analytical approach of methylation and nuclear magnetic resonance techniques have been successfully 6 Bangladesh J Pharmacol 2016; 11: 1-23 F ig u re 3 A : C h em ic a l st ru ct u re s o f so m e o f se le ct ed i n d u st ri al h o m o -e x o p o ly sa cc h a ri d es p ro d u ce d b y l a ct ic a ci d b a ct er ia ( L A B ). a : L ev a n ( fr u ct a n s) ; b : I n su li n -t y p e fr u ct a n ; c : D ex tr an ( g lu ca n ); d : M u ta n ( g lu ca n ); e : A lt er n an ( g lu ca n ); f : G el la n Bangladesh J Pharmacol 2016; 11: 1-23 7 F ig u re 3 B : C h em ic a l st ru ct u re s o f so m e o f se le ct ed i n d u st ri al h et er o -e x o p o ly sa cc h a ri d es p ro d u ce d b y la ct ic a ci d b a ct er ia ( L A B ). a : X a n th a n ; b : H y a lu ro n a n ; c: C h o n d ro it in s u lf at e; d : S u cc in o g ly ca n ; e : .H ep a ri n ; f: K er ta n s u lf a te 8 Bangladesh J Pharmacol 2016; 11: 1-23 used to determine the linkage pattern of polysaccharide monomers (Le Costaouec et al., 2012). In the current scenario of molecular mass and spectroscopic data analysis, nuclear magnetic resonance techniques have provided most accurate data for complex poly- saccharide molecules (Duus et al., 2000). It has been reported that two-dimensional nuclear magnetic resonance spectroscopic technique has been successfully able to determine the primary chemical structure of exopolysaccharides of microbial origin (Leeflang et al., 2000). Application of known spectros- copic techniques is gaining more importance in the field of chemical structure analysis along with an additional efforts on to develop more advanced techniques for the easy and accurate determination of large molecular mass carbohydrate or polysaccharide molecules. In this regard, spin diffusion technique, a complement to the hetero nuclear multiple bond correlation, has provided positive results on the determination of linkage pattern of exopolysaccharide monomers (Vincent and Zwahlen, 2000). Recently suggestions have been made on the use of deuterium-induced differential isotope shifts to analyze the linkage pattern of exopolysaccharides monomers which can be a good substitute to two- dimensional nuclear magnetic resonance technique and shaped pulses since these techniques result in the deletion of methylation step, leading to direct inspec- tion of nuclear magnetic resonance of polysaccharide complex molecules (Navarini et al., 2001; Bendiak, 2002). In general, analysis and differentiation of different exopolysaccharides in various taxa of different groups of lactic acid bacteria by traditional and conven- tional methods assess the number of monosaccharides present in the repeating backbone units of oligosaccha- ride molecules which reflect the sugar residual numbers in the main oligosaccharide chain. However, a macroscopic identification can assist to determine the similarity among the variety of exopolysaccharides based on the similar structural writing pattern and set of rules for presenting exopolysaccharide structures. Genetics and biological synthesis of exopolysaccha- ridein lactic acid bacteria Lactic acid bacteria have potent ability to synthesize varied classes of polysaccharides assisting them to construct essential components of cell wall. Lactic acid bacteria synthesize polysaccharides using various known biosynthetic mechanisms correlated with cell wall component mechanisms (Welman and Maddox, 2003). The functional properties and biosynthetic ability of bacteria specially cell wall components were review- ed previously. In general, biosynthesis of exopoly- saccharide is an energy-dependent process which utilizes one mole of adenosine triphosphate leading to conversion of each hexose substrate molecule to hexose phosphate followed by the utilization of high energy phosphate bond for the biosynthesis of each nucleotide sugar molecules. Further, this process requires another one mole of adenosine triphosphate energy, needed for the phosphorylation of the lipid carrier and for the transportation and polymerization of polysaccharide molecule. Since lactic acid bacteria have ability to generate energy in limited amount, this may result in the limited production of exopolysaccharide by lactic acid bacteria (de Vuyst and Degeest, 1999). However, production of exopolysaccharide is directly dependent on the growth of bacterial cells and can be well explained by the combined chemistry of cell growth parameters and their ability on exopoly-saccharide biosynthesis (Cerning et al., 1992). The exopolysaccharide biosynthesis represents a very complexprocess involving the functional ability of number of genes assisting in the EPS biosynthesis. These exopolysaccharide related genes of plasmid and chromosomal regions specifically in mesophilic and thermophilic lactic acid bacteriasuch as Lactococcus, Streptococcus and Lactobacilli encode various enzymes and proteins, and importantly participate in the exopolysaccharide biosynthesis (Van Kranenburg et al., 1997; de Vuyst and Degeest, 1999). Genetic analysis of S. thermophiles Sfi6 resulted in the identification of exopolysaccharide locus with a 15.25kb region which encoded 16 open readings to different lactic acid bacteria (Lamothe et al., 2002; Stingele et al.,1996). The orientation of gene cluster was unidirectional and each gene was transcribed as single mRNA (Jolly et al., 2001). However, mesophilic isolates of lactic acid bacteria have shown instability on exopolysaccharide production due to the unstable nature of plasmid region genes which specially encode various enzymes for exopolysaccharide biosynthesis in mesophilic lactic acid bacteria. On the other hand, deletions and genetic re-establishments in lactic acid bacteria may affect the exopolysaccharide production ability of thermophilic bacteria significantly. Moreover, a number of other housekeeping genes encoding various enzymes needed for the formation of sugar nucleotides also play an important role in the molecular biosynthesis of exopolysaccharide in lactic acid bacteria. An overview on biosynthetic ability of exopoly- saccharides by lactic acid bacteria has been given in Figure 4. As demonstrated, the biosynthesis of glucose- 1-phosphate results in the production of UDP-glucose, UDP-galactose, and dTDP-rhamnose nucleotide sugars which are attached to phospholipids on the lipid carrier and assemble into sugar units. Further, transfer of sugar nucleotides diphospho-precursors to a carrier lipid results in the formation of basic repeat unit, where lipid carrier plays a pivotal role on assembling of repeating sugar units (Kleerebezem et al., 1999). In addition, analysis of lipid-linked oligosaccharides (Peant et al., 2005) has confirmed that biosynthesis of heteropolysaccahirdes begins with the formation of EPS monomers such as intracellular exopolysaccharide Bangladesh J Pharmacol 2016; 11: 1-23 9 precursors, uridine-59-diphosphate-galactose, uridine- 59-diphosphate-glucose and deoxythymidine diphosphate-rhamnose, leading to the biosynthesis of most repeating units (Peant et al., 2005). Role of different exopolysaccharide encoding genes has been confirmed previously in various lactic acid bacteriaat genus and species level (Broadbent et al., 2003; Jolly et al., 2001, 2002; van Kranenburg et al., 1997). Generally microorganisms produce number of substances as a mixture of polymers which are synthesized through specific genes located on gene cluster (Hay et al., 2010; Orr et al., 2009; van Kranenburg et al., 1999). Although vast majority of genetic information is available on xanthan, related information on other types of exopolysaccharides is rare. The exopolysaccharide biosynthesis of xanthan is controlled by gum genes in Xanthomonas campestris located on a single gene cluster of 12 kb (Vorholter et al., 2008), where nucleotide sugars of uridine-59-diphosphate-glucose, uridine-59- diphosphate-glucoronate and guanosine-59- diphosphate-mannose make the repeating units of xanthan, leading to enhanced production of xanthan exopolysaccharide (Vorholter et al., 2008). In addition, gum genes (D, H, I, K, M) also encode the biosynthesis of glycosyltransterase (van Kranenburg et al., 1999; Vorholter et al., 2008). Recently it has been reported that in Pseudomonas aeruginosa, 12 gene cluster in a single operon encodes the production of alginates (Hay et al., 2010). However, a gum gene (algC) involved in EPS production was found to locate on chromosomal region which encodes phosphomannomutase (Hay et al., 2010). Although limited information is known on molecular mechanism of exopolysaccharide biosynthesis, genetic establishments such as genes encoding regulation, repeating-unit assembly, chain- length determination, polymerization and exportation play a very important role in exopolysaccharide biosynthesis especially in Gram-positive bacteria (de Vuyst and Degeest, 1999; Peant et al., 2005; Jolly et al., 2001). Way of organization of exopolysaccharide- regulation genes in some of the selected lactic acid bacteriahas been illustrated in Figure 5. Figure 4: Biosynthesis of exopolysaccharide (EPS) in lactic acid bacteria (LAB). 1st step: Transportation of sugar into the cytoplasm; 2nd step: Synthesis of glucose-1-phosphate; 3rd step: Sugar nucleotides syn- thesisand polymerization into EPS subunit; 4th step: Export out of the cytoplasm; 5th step: Polymerization and detachment of EPS [Kleerebezem et al., 1999] (Lipid carrier (step 3) (step 4) UDP-glucose UDP-galactose dTDP-rhamnose Glucose -1-phosphate dTDP-glucose dTDP-4k-6D mannose Glucose - 6-phosphate Glucose + Galactose- 6– phosphate Glucose + Galactose Lactose - 6 - phosphate Py ro ph os ph at e R es ul tin g EP S Lactose (Step 1) Lactose Lactate (step 5) 10 Bangladesh J Pharmacol 2016; 11: 1-23 It is obvious to say that in-depth studies are certainly required to provide innovativeinsights on the exopoly- saccharide biosynthesis pathways and theirgenetic regulation for the enhanced production of quality-rich EPS (Welman and Maddox, 2003). Multitude of combined experimental skills with an integrated molecular approach on metabolic biosynthetic pathway will probably require in order to bring a significant production rate of exopolysaccharide from lactic acid bacteria. In addition, in silico production, genetic modifications, gene expression level studies, molecular modeling and application of advanced techniques may also help to enhance on exopolysaccharide biosynthesis from lactic acid bacteria (Welman and Maddox, 2003). Since lactic acid bacteria represent a vast group of bacteria with multitude of industrial applications, successful exploitation of these group of bacteria in terms of their potent ability on exopolysaccharide biosynthesis and their various functional and health beneficial effects will certainly lead to innovative industrial and pharmacological applications. Industrial and pharmacological applications of exo- polysaccharide Microbial exopolysaccharides, molecules of diverse chemical composition,have shown multitude of functional and health beneficial effects with enormous range of applications in food, cosmetic, agronomy and pharmaceutical industries. Since lactic acid bacteria- based products ascertain various functional properties, application of EPS can be exploited in the food industry with reliable amount of yields and recovery rate (Patel et al., 2010; Patel et al., 2012). Moreover, production of exopolysaccharides from lactic acid bacteria can be economically feasible if production parameters and other factors which affect the exopolysaccharide pro- duction could be optimized such as cheaper substrates and cost-effective fermentation conditions (Patel et al., 2010; Patel et al., 2012). A detailed description on recent progress pertaining to various functional and health beneficial properties of lactic acid bacteria and lactic acid bacteria-based exopolysaccharides has been summarized in Table I. A number of importantapplicationsof lactic acid bacte- ria-based exopolysaccharides have been developed for their commercial exploitation especially with health benefit purposes and considerable amount of progress has been made for discovering novel types of microbial exopolysaccharides for industrial purposes (Tieking et al., 2005; Kumar and Modi, 2009). Moreover, lactic acid bacteria-basedexopolysaccharides have shown remarkably positive effects on gut health, as well as exhibit antitumor effect, cholesterol lowering effect, and immunomodulatory effect (Madhuri and Prabhakar, 2014). The exopolysaccharide derived from Bacillus licheniformis was found to diminish the replication of herpes simplex virus-2in human peripheral blood mononuclear cells exhibiting antiviral effect in vivo. In addition, non-digestible oligosaccharides as prebiotics have also been found to stimulate the growth and activity of health beneficial bacteria including Bifidobacteria and Lactobacilli in the colon, thereby show health benefits in human host (Harutoshi, 2013). Also exopolysaccharides can facilitate the colonization of gastrointestinal tract by lactic acid bacteria and Bifidobacteria due to their prolong survival ability in gastrointestinal tract. Besides, exopolysaccharides Figure 5: Systematic way of organization of exopolysaccharide(EPS) genes in some LAB species (Lactococcus lactis) [Van Kranen- burg et al., 1997; Welman andMaddox, 2003] Polymerization chain length Export Rha Rha Glc Glc - Glc Glc - Glc - Gal Glc - Glc - Gal Glc - Glc - Gal Polymerization 1 kb Gal-P IS R X A B C D E F G H I J L orfY K Bangladesh J Pharmacol 2016; 11: 1-23 11 Table I List of different EPSs isolated from LAB strains showing various functional properties Name of EPS Source strain Structure Application and biological importance References Reuteran Lactobacillus reuteri LB 121 Lb. reuteri ATCC 55730 Lb. reuteri 35-5 -1,4 linkage, also -1,6 glycosidicbonds Used in bakery Tieking and Ganzle, 2005 Kralj et al., 2005 Inulin Lb. johnsonii NCC 533. Streptococcus mutans JC2, Leuc. citreum CW28 Lactobacillus reuteri 121 -1,2 glycosidic bonds Prebiotics, nourish gut mucosal cells and inhibit pathogens, targeted drug delivery against colon cancer, substitute of fat in food products Buchholz and Seibel, 2008 Sartor , 2004 Kefiran Lb. kefirgranum Lb. parakefir Lb. kefir Lb. delbrueckii subsp. bulgaricus Glucose and galactose monomers form variable acidic bonds Improve viscoelastic prop- erties of acid milk gels, antimicrobial and wound healing properties, ability to lower blood pressure and cholesterol in serum, capacity to retard tumor growth, enhance immunity of gut Micheli et al., 1999 Medranoet al.,2008 Vinderola et al., 2006 New EPS Lb. johnsonii 6)- -D-Galp-(1 6)- -D- Glcp- (1 3) - -D-Gal f - (1 3)- -D-Glcp-(1 2)- - D-Galf-(1 Immune-reactivity Gorska-Fraczek et al., 2013 OLL1073R-1 L. delbrueckii sp. bul- garicus - Anti-IFV IgA, IgG1 in BAL NK cell →ctivity (IFV = influenza virus; BALF = bronchoalveolar lavage fluid) Nagai et al., 2011 Novel hetero -EPS S.phocae PI80 Larger number of (O-H), (C=O), (C-H) stretching frequency and carboxyl group. Combination of arabinose, fructose and galactose monomers Emulsifying and flocculat- ing activities, antioxidant, anti-biofilm Kanmani et al., 2011 -glucan Pediococcus damnosus 2.6 Dglcose monomers linked by -glycosidic bonds Starter culture Martensson et al., 2000 New EPS Leuconostoc sp. CFR 2181 (1 → 6) linkage Glucose, rhamnose and arabinose Starter culture Vijayendra and Babu, 2008 Glucan Lactococcus lactis 1.8 Capsular - Van der Meulen et al., 2007 and -glucans Lb. brevis G-77 - Starter culture Glucan Leuc. citreum 1.10 Non-capsular - Van der Meulen et al., 2007 Hetero-EPS Lb. curvatus 10 Galactosamine, galactose, glucose - Van der Meulen et al., 2007 Homo-EPS-3 Lb. plantarumLP6 71.3% carbohydrate con- tent Antioxidant Li et al., 2013 12 Bangladesh J Pharmacol 2016; 11: 1-23 render unique features including alleviation of lactose intolerance, immunity enhancement against harmful pathogens and reduction of mutagenic enzymes such as -glucuronidase, nitroreductase and chologlycine hydrolase (de Roos and Katan, 2000). In addition to the ability of exopolysaccharides as food additives, they have shown improved specificity of drug release in the treatment of colon cancer. EPSs which serve as substrates for colon microflora, have also been utilized successfully in drug conjugation as well as coating and matrix agents (Vandamme et al., 2002). It has been reported that few selected exopolysaccharides have been found to display B-cell mitogen like activity along with an ability to modify the functions of macrophages and splenocyte (Kitazawa et al., 2000). An exopoly- saccharide derived from L. johnsonni isolated from intestinal tract of mice displayed broad range of immunity reactions (Gorska-Fraczek et al., 2013). Bifidobacteria and Lactobacilli conferred to be as health promoting bacteria have potential ability to inhibit pathogenic bacteria and stimulate host immune system dramatically (Mitsuoka, 1992). In general, microflora of gastrointestinal tract consist of about 1014 colony forming unit/g of different types of both pathogenic and beneficial bacteria with diverse range of composi-tional variations along the gastrointestine (Mitsuoka, 1992). Equilibrium of gastrointestinal microflora signifi-cantly affect the host health in terms of smooth bowl moment, tympanites flatulence and nutrient absorption (Harutoshi, 2013). A number of factors might have detrimental effect on this microflora equilibrium such as a biotic or biotic stress, antibiotic consumption, infection, food poisoning, and the natural ageing process. However, to diminish this detrimental effect and to maintain the proper growth and biological activities of these beneficial microflora, supply of specific ingredients in consumable food can provide significant improvement (Harutoshi, 2013). Further, levans, group of fructans, have been used in food, pharma and cosmetic industries with special emphasis in food industry as a food and feed additives when used with prebiotics (Kang et al., 2009). Levans and dextrans have also been found to exhibit hypocholestrolemic effect (Kang et al., 2009). In addition, alginates have been used as thickening agents in food industry (Remminghorst et al., 2009). Also xanthan has showed its potent ability being used as a viscosifer, thickener, emulsifier or stabilizer in the food industry (Becker and Vorholter, 2009). Exopoly- saccharides of microbial origin have been known for their versatile rheological properties which make them suitable candidates for using in food industry especially in variety of yoghurts with a high purity rate (Tieking and Ganzle, 2005). As reported previously, fructose- Table I (Cont.) List of different EPSs isolated from LAB strains showing various functional properties Name of EPS Source strain Structure Application and biological importance References Neutral EPS Lb.fermentum TDS030603 D-gl ucose a nd D - galactose, terminal D- Glcp, 3-substituted D-Glcp, 2,3-disubstituted D-Glcp, and 6-substituted D-Galp - Gerwig et al., 2014 EPS Lb. rhamnosus KL37B Non-saccharide repeating units - Gorska-Graczek et al., 2011 Kefiran LAB D-glucose (Glc), D- galactose (Gal) and D- xylose (Xyl) - Zajsek et al., 2013 Neutral EPS Lb. johnsonii 142 3)- -D-Galp-(1 3)- -D- Glcp- (1 5) - -D-Gal f - (1 3)- -D-Galp-(1 3)- - D-Galp-(1 - Gorska et al., 2010 Glucan Weissella cibaria MG1 e (a-1,6) and glucooligo- saccharide Adjunct culture in cheese Lynch et al., 2014 EPS Lb. casei C12 Heteropolysaccharide Adjunct culture in cheese Glucan Lb. reuteri ff2hh2 homopolysaccharide ( - 1,6 and -1,4) and fructooligosaccharide Adjunct culture in cheese Glucan Lb. reuteri cc2 Homopolysaccharide ( - 1,6 and -1,4) and fructooligosaccharide Adjunct culture in cheese Bangladesh J Pharmacol 2016; 11: 1-23 13 oligosaccharides, low calorie and low sugar compo- nents have various applications in food industry with a status non-carcinogenic (Yun, 1996). Also based on the prebiotic properties of inulin and fructose-oligo- saccharides, they have been used in food industry (Rhee et al., 2002). Moreover, polymers of fructose, fructans have significant role in plant cellular stress tolerance by protecting membrane stabilization (Oliver et al., 2001). In addition, dextran has ability to protect the producer strain during starvation or adverse conditions such as an alkaline or acidic state (Kim et al., 2007). Since fructose-oligosaccharides and exopolysaccharides have ability to protect bacteria from adverse stress conditions, they might have practical application in food industry to protect health beneficial lactic acid bacteria in fermented food products and storage of starter cultures (Tieking and Ganzle, 2005). Moreover, a number of other important roles of microbial exopolysaccharides have been reported recently (Liang and Wang, 2015). Various exopolysaccharides have been found to protect microorganisms from dehydration, phagocytosis, predation, bacteriophage attack, and from the adversary effects of antibiotics and toxic compounds (Roberts, 1996). Proper exploitation of lactic acid bacteria could be a faceable approach to produce versatile exopolysaccharide polymers for their practical applications (Frengova et al., 2002). Microbial EPSs have been found to display number of biological activities including antimicrobial, antiulcer, antitumor, immunomodulatory, and cholesterol lowering effect (Welman and Maddock, 2003). Recently utilization of coconut waste water has been exploited in Thailand for the production of exopoly- saccharide using an efficient strain L. confuses TISTR1498 leading to limit the coconut waste water supply as a waste to an extended amount (Seesuri- yachan et al., 2011). Tieking and Ganzle (2005) reported the beneficial importance of lactic acid bacteria-derived exopolysaccharidesin dough and bread which may affect their technological properties in various aspects such as water absorption properties, rheological proper- ties, stability during frozen state, loaf volume, and staling. In addition, polymers of exopolysaccharide have been successfully used in backing applications to improve the metabolic traits, flavor, texture and shelf- life of bread (Tieking and Ganzle, 2005). A detailed overview on the industrial and pharmacological usefulness of microbial exopolysaccharides has been summarized in Figure 6. Figure 6: Multifarious applications of microbial exopolysaccharides (EPS) in various industries - Purification of compounds of medical interest - As a vaccine - Maintenance of gut health - Surgical sutures - Anticoagulants - Laxative - Cholesterol lowering ability - Controlled drug release - Control viscosity and modify flow - Improve texture - Improve mouth feel - Improve freeze-thaw stability - Thickeners softeners - Suspending agents - Antiviral - Antimicrobial - Anti - inflammatory - Immunomodulatory - Antitumor - Anti - infective - Pesticide - Wound healing ability - Influence on antibiotic stress - Genetic regulation in human pathogenesis - Therapeutic interventions - Human immunity enhancers - Functional properties - Food additives in bakery products - Limiting factor for biofuel production - Low calories food products - Dietary fibers - Films and coating agents - Salad dressings - Frozen food icing - Moisturizing agents - Purification of enzymes for diagnostic purposes - Encapsulation drug delivery system - Chemical modification of pharmaceutical drugs - Coating, lotions - Enzyme innobilization - Immune modulation - Blood volume expanders - Capacity for gel formation - Flocculent - Lubricant - Dye and pigment suspensors - Suspending agents in ceramics glazes - Rheology modifier in paper and pulp - Soil conditioners - Physical and chemical properties - Rheological capacity - Improve sensory attributes - Texturizers - Stabilizers - Viscosifers - Elasticity - Gelling agents 14 Bangladesh J Pharmacol 2016; 11: 1-23 Polysaccharides as microbicides It has been observed that a polymer group of sulfated polysaccharides has shown significant biological and functional properties (Ghosh et al., 2009). Since these sulfated polysaccharides as macromolecules display imitate patterns of sulfate substitution on glycosami- noglycans present in cell membranes, they have been found to be potent antiviral agents. Generally sulfated polysaccharides represent a complex polymer form of heterogeneous composition. Although chemical struc- ture and activity relationship of sulfated polysaccha- rides have not been studied in detail, a number of these macromolecule even in crude forms have shown potent antiviral effect (Ghosh et al., 2009). Microbicides are biocidal compounds or substances which reduce the infectivity of microbes, such asvirusesorbacteria. Generally microbicides are applied vaginally or rectally and protect the user from sexually transmitted infections caused by pathogenic fungi, viruses or bacteria. High molecular weight electrolytes, polyanions, are considered to be the most effective microbicides. A structural-activity relationship study of sulfated polysaccharides has confirmed the potent efficacy of these macromolecules as antiviral agents in terms of their ability to serve as microbicides (Ghosh et al., 2009). Clinical trials offer justified approach of polysaccha- rides to be used as microbicides in future generations. These approaches include the synergistic or combina- tory effect of various drugs especially in the initial inhibition of viral infection steps. Since lactic acid bacteria-derived sulfated polysaccharide confer safe and acceptable status on topical applications, strategies on developing next-generation microbicides using sulfated polysaccharides alone or in combination may give much better results on curing sexually transmitted diseases in terms of their antiviral or antimicrobial potency (Bollen et al., 2008; Kilmarx et al., 2008; Brache et al., 2007; Klasse et al., 2008). A microbicide (PC-815) developed via the combined mixture formulation of carrageenan and the nucleoside reverse transcriptase inhibitor MIV-150 has confirmed its efficacy as a next- generation microbicide (Woolfson et al., 2000; Malcom et al., 2005). However, successful application of these polysaccharide-based microbicide formulations has become a bloom and critical issue on these inhibitors for proper vaginal delivery system. Exopolysaccharides as antiviral drugs and target Figure 7: Schematic presentation of various antiviral mode of actions of probiotics including inhibition of virus by direct binding of viral particles (a); blockage of viral attachment by steric hindrance, blockage of cover receptor sites in non-specific manner (b); induction of mucosal regeneration thus binding virus particles and inhibit adherence to epithelial cells leading to inhibition of virus replication (c); production of antiviral compounds (d); antiviral effect through production of nitric oxide and dehydrogen- ase (e); promotion of normalization of mucosal barrier and enhancement in integrity of mucosal cells (f); epithelial immune re- sponse modulation (g); immune response modulation through macrophages (h); antiviral effect through activation of CD + T lymphocyte differentiation into cytotoxic T lymphocytes (i); antiviral effect by phagocyte promotion through Th1 activation (j); through induction of B cells proliferation (k) and antiviral or viral neutralization effect through secretion of antibodies (l) [Lehtoranta, 2012] a b d e c m Probiotic NO- H2 O- Lume Epithelium Lamina propria MALT IgM IgG Ig Macrophag Phagocyte activation K I j CD4+ B cell Th1 Plasma Macrophage Th2 NK cell CTL g h i CD8 f M cell Bangladesh J Pharmacol 2016; 11: 1-23 15 http://en.wikipedia.org/wiki/Virus http://en.wikipedia.org/wiki/Bacterium modes of action Lactic acid bacteria have shown great potential in the prevention of severe gastrointestinal disorders in human beings and animals (Ohet al., 2010; Kumar et al., 2010). Although the mode of antiviral action of lactic acid bacteria has not beenelucidated in details, they have shown significant ability to inhibit viral infections and/or replicationeither directly or indirectly caused by respiratory, gastroenteric, murine, influenza, herpes and Newcastle disease viruses (Oh et al., 2010; Seo et al., 2010; 2012; Lange-Starke et al., 2014; Kassaa et al., 2014;). However, variations in the antiviral effect have been observed at species level based on the efficiency and biological properties of the test strain. Generally, mode of action of lactic acid bacteria as antiviral agents accomplished in four major steps which include i) adsorption hindrance, ii) internalization of virus in the cell, iii) production of antiviral substances, and iv) establishment of antiviral effect through immuno- modulation or cross-talk. An outline of possible antiviral modes of action of lactic acid bacteria has been summarized in Figure 7. Exopolysaccharides are known as potentially useful and biologically active polymer substances for medicinal and pharmaceutical uses due to their versatile biological properties. Liu et al., (2004) reported that polysaccharide may prevent viral infection through blockage of virus adsorption onto the host cells by interacting either with virus particles or with the host cell. This study confirmed that strong evidences on interaction of polysaccharide molecule and cell membrane should be occurred in order to confirm the proper blocking of receptor resulting in the adsorption of virus on the cell membrane (Liu et al., 2004). Among the tested polysaccharides, sulfated polysaccharides have shown potent ability to display antiviral effect (Oh et al., 2010). Inhibition of virus-cell adsorption onto the host cell is considered to be the first steps in viral infection process. It has been found that sulfated polysaccharides inhibit the virus-cell attachment and display antiviral effect against various types of viruses including hepatitis B virus, human cytomegalovirus, herpes simplex virus and influenza virus (Oh et al., 2010). Hence, in the current scenario of antiviral research, lactic acid bacteria and their derived polymers or polysaccharides are considered potential candidates in antiviral therapy to prevent or treat viral infections in both human and animals with remarkable efficacy and might have significant contribution in medicine and pharmaceutical industries in future (Oh et al., 2010). An effective antiviral substance or compound has ability to prevent the cells from viral infections either directly by inactivating the virus particles or by interfering the replication cycle of virus. However, intracellular activity of any antiviral drug should be interpreted by excluding the possible capability of inactivating the virus extracellularly. A protein bound microbial polysaccharide showed potent ability to reduce the viral titer of herpes simplex virus-1 and herpes simplex virus-2 viruses (Eo et al., 2000). Although no precise antiviral mode of action of protein bound polysaccharide was defined, the significant antiviral activity against herpes simplex viruses might be attributed to the following reasons including disintegration of the entire herpes simplex virus particles, solubilization of the virus envelope, modifi- cation in chemical composition, protein degradation, or by covering the essential proteins of virus envelop (Eo et al., 2000). This study revealed that protein bound polysaccharide may exert its partial antiviral mode of action against herpes simplex virus-2 virus via binding the glycoproteins of herpes simplex virus-2 virus to protein bound polysaccharide which may interfere at the initial state of virus replication or release of any virions. Similarly the sulfated polysaccharides dextran sulphate and heparin showed remarkable antiviral activity via their interaction with virus particle attach- ment to the host cell (Bouhlal et al., 2011). In addition, other sulfated polysaccharides including pentosan, polysulphate, sulphated cyclodextrins, xylofuranan sulphate, ribofuranan sulphate and mannan sulphate have also been found to exhibit inhibition of viral replication process for herpes simplex virus, human cytomegalo virus and human immunodeficiency virus (Bouhlal et al., 2011). Polymers of exopolysaccharide have shown potent antiviral effects, however, concerns have been raised on their acceptability due to their being relatively large molecular weight compounds and with a phenomenon that they block virus attachment, an unfavorable step in antiviral therapy, making them the molecules of uncertainty that they could pass through the different barriers of the body even cell membrane (Bouhlal et al., 2011). Large polymer molecules might be difficult to penetrate to certain extent when using in topical application for the treatment of specific cutaneous herpes simplex virus-1 viral infections, small oligosaccharide polymers such as dextran sulfate have ability to pass through easily from different body barriers. Also these oligosaccharides have shown better diffusion ability in blood when fed in the experimental rats with oral heparin (Li et al., 2011). This study confirmed that oligosaccharide polymers such as dextrans possessing sulfate group might serve as natural alternatives in antiviral therapy leading to proper utilization of these inexpensive polysaccharides found abundantly in nature (Bouhlal et al., 2011). How- ever, presence of other functional group other than sulfate might also have possibilities on the efficiency and antiviral properties of other types of polysaccha- rides. It has been reported that polysaccharides such as 16 Bangladesh J Pharmacol 2016; 11: 1-23 carrageenan inhibit antiviral effect via inhibition of viral protein synthesis process only when it is present at early stage of virus particle entry (Bouhlal et al., 2011). In addition, although sulfated polysaccharides have shown their ability to reduce the number of viral plaques when used in crude form, the virions can internalized even at a 10-times higher concentration of carrageenan, necessary to block the early step of viral replication. This indicated that although virus internalization occurs, the viral replication can be blocked even in the absence of carrageenan confirming that perpetration was not solely acceptable. In contrast, lactic acid bacteria or lactic acid bacteria-derived polysaccharides show diverse range of biological properties. Recently, L. brevis KB290, a lactic acid bacterium with known immunomodulatory properties shown potent antiviral effect against influenza virus in experimental mice models (Waki et al., 2013). The results confirmed that KB290 when administered orally alleviated influenza virus-induced clinical symptoms which might be induced by long-lasting enhancement of interferon- production and the augmentation of influenza virus-specific immunoglobulin-A production, suggesting that KB290 might have exopolysaccharide like component responsible for this antiviral effect (Waki et al., 2013). Also Kawase et al., (2010) reported that a Lactobacilli strain isolated from human intestinal tract prevented viral infection caused by influenza virus. An exopolysaccharide isolated from a probiotic strain L. bulgaricus OLL1073R-1 showed potent immuno -stimulatory effect (Makino et al., 2006; Nishimura- Uemura et al., 2003). Further, the effect of yogurt fermented with OLL1073R-1 on the reduced risk of respiratory infections was visualized in elderly following cohort studies (Nagai et al., 2011; Makino et al., 2010). It was found that fermented yogurt augmented the NK cell activity in the subjects who had lower NK cell activity, confirming that administration of exopolysaccharide containing yogurt might have potential role to prevent the infections of influenza virus since lymphocyte natural killer cells of innate immune system have crucial role in the early host defense against various viral infections (Makino et al., 2010; Viver et al., 2008). Previously reported studies confirmed that first- generation antiviral drugs showed less potency in preventing the viral vaginal infection caused by human immunodeficiency virus-1 transmitted virus. However, polysaccharides as second-generation drug alone or in combined formulation with other polysaccharide or drugs have shown broad range of antiviral spectrum leading to provide a sustainable antiviral therapy against vaginal pathogenesis (Ghosh et al., 2009). Lactic acid bacteria and lactic acid bacteria-derived polysaccharide specifically sulfated polysaccharides have several advantages over varied classes of synthetic and antibiotic antiviral substances having severe side effects. These polysaccharides have relatively low production cost, exert broad range of antiviral efficacy, show low range of toxicity, display low range of viral drug resistance, and show high lyophilicity, making them the candidates of promising antiviral drug discovery in near future with safe and widely acceptable levels which show precise modes of antiviral action. Future prospects Numerous studies have confirmed that lactic acid bacteria have potent ability to produce various types of chemically complex poly- and oligosaccharides. A plethora of information on the exopolysaccharide production ability of various Lactobacilli along with their diverse role in ecosystem have been well reviewed in the literature. However, efficacy on low amount of production by these Lactobacilli has limited their application as food grade additives. Influenced with these hurdles in exopolysaccharide production from lactic acid bacteria, studies on optimized parameters for efficient fermentation in combination with molecular and metabolic engineering techniques may help to achieve higher amount of exopolysaccharide biosynthesis by lactic acid bacteria. Moreover, a detailed information on the chemical and structural relativity relationship might help to provide microbial exopolysaccharides a new way for their practical applications in various desirable fields to be used as natural health promoters with diverse range of biological properties. Although pivotal information at molecular level for some of the selected lactic acid bacteria on metabolic pathways involved in exopolysaccharide biosynthesis, gene sequencing with well-established chemical composition profile are available, this lacks the information on mechanisms of the secretion of microbial exopolysaccharides from bacterial cells as well as their relationship with genomic sequences and related enzymes responsible for exopolysaccharide production. Hence, future studies using advanced molecular techniques on this industrially-sound group of exopolysaccharides may result in the detailed view of the secretion of exopolysaccharides from microbial cells which may provide a scientifically innovative look leading to their enhanced production. In near future, gene expression level studies and molecular techniques can give new insights on enhanced production of microbial exopolysaccharides under controlled chemical environment leading to more functional and versatile exopolysaccharide production. Also the types of suitable methods can be a good target for obtaining optimized parameters for higher amount of exopoly- saccharide production for microbial community. In this regards, exopolysaccharide producing lactic acid bacteria could be served as potent and natural microbial sources as efficient starter cultures with predictable Bangladesh J Pharmacol 2016; 11: 1-23 17 chemical characteristics which may contribute significantly to develop innovative approaches for the enhanced production of various types of exopoly- saccharides added with new and additional functional tracts of biological efficacy. Concluding remark Microbial exopolysaccharides have attracted huge attention in various industries especially in food, dairy and cosmetic industries due to their versatile functional and biological properties. Microbial exopolysaccharides have also been used in medicine and pharmaceutical industries making them important tools in drug delivery system and delayed drug released formulation. A number of selected microbial strains which produce exopolysaccharides efficiently have shown additional metabolic features, health beneficial effect and significantly affect texture, aroma, and shelf- life of various food products. Apart from, exopolysaccharides have shown diverse range of industrial applications, they have been shown potent role in cellular recognition as well as found to protect the microbial cells from various adverse conditions such as osmotic stress, solid surface adhesion, protozoa predation, phagocytosis, phage attack, antibiotics or toxic compounds. Moreover, microbial exopoly- saccharides have been successfully applied in the food industry as a bio-thickeners, viscosifers, as well as stabilizing and emulsifying agents. Since exopoly- saccharides display multitude of biological and functional properties, when used as prebiotics, they can stimulate the function and growth of beneficial colon microflora thereby improving the host health system. Also exopolysaccharide polymers vary in their chemical nature, any specific functional property is positively correlated to the chemical structure of exopolysaccha- rides. Exopolysaccharides of microbial origin display wide range of chemical diversity and functions and their production is not limited to a group of species. For example, they display monomeric compositions, lin- kage bonds and associated conjugates whereas the functions include intrinsic and applications. The major intrinsic functions of exopolysaccharides include morphological, structural and protective efficacy. However, exopolysaccharides can also be applied for human purposes in medical, cosmetic, food, pharma and dairy industries along with their efficacy in other industrial and environmental prospects. Although diverse range of microbial exopolysaccharides represent a huge amount of practical applications, specifically it is vital and necessity of these exopolysaccharide for human uses since they confer GRAS status as well as show cost effectiveness when used in environmental application for neutralizing toxic constituents. In non-industrial applications, cost of exopolysaccharide production has become a major limiting factor especially in case of bio-flocculation process. Apart from this, experimental efforts are still needed on searching efficient bacterial strains, enough capable to produce exopolysaccharides and exopolysaccharide-conjugates with higher yields that can meet their industrial need. In addition, use of cost- effective and cheap substrate materials, optimization of fermentation condition in combination with genetic and metabolic engineering approaches might serve as effective tools for the enhanced production of functional exopolysaccharides for industrial applications. Consequent inflation and inefficient downstream processing have also limited the exopolysaccharide production which has become a major hurdle in exopolysaccharide exploitation at industrial level. However, sufficient genetic information on exopolysaccharide biosynthesis and bioprocess manipulation strategies may assist to overcome such less exopolysaccharide production recovery. On the other hand, low awareness of consumers on exopolysaccharides also is a limiting factor for their commercial exploitation. Strict international laws and legislations have also affected significantly on the commercial usefulness of microbial exopolysaccharides. 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Bajpai Kangmin Kim Sun-Chul Kang Yong-Ha Park First two authors contributed equally e-mail: vbajpai04@yahoo.com activase@jbnu.ac.kr sckang@daegu.ac.kr peter@ynu.ac.kr Bangladesh J Pharmacol 2016; 11: 1-23 23 http://www.ncbi.nlm.nih.gov/pubmed?term=Vorh%C3%B6lter%20FJ%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Schneiker%20S%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Goesmann%20A%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Krause%20L%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Bekel%20T%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Kaiser%20O%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Linke%20B%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Patschkowski%20T%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=R%C3%BCckert%20C%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Schmid%20J%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Sidhu%20VK%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Sieber%20V%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Tauch%20A%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Watt%20SA%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Weisshaar%20B%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Becker%20A%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Becker%20A%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=Niehaus%20K%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed?term=P%C3%BChler%20A%5BAuthor%5D&cauthor=true&cauthor_uid=18304669 http://www.ncbi.nlm.nih.gov/pubmed/18304669 Your feedback about this paper 1. 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