Bangladesh Journal of Pharmacology Volume: 10; Number 4; Year 2015 Cite this article as: Swamy MK, Sinniah UR. Phytochemical profile and in vitro α-amylase inhibitory potential of different solvent extracts of Lantana camara. Bangladesh J Pharmacol. 2015; 10: 962-63. http://www.bioxbio.com/if/html/BANGL-J-PHARMACOL.html Phytochemical profile and in vitro α-amylase inhibi- tory potential of different solvent extracts of Lantana camara Sir, Methanolic leaf and fruit extracts of Lantana camara were reported to possess antihyperglycemic property in both streptozotocin- and alloxan-induced diabetic rats (Ganesh et al., 2010; Kazmi et al., 2012; Venkatachalam et al., 2011). However, α-amylase inhibition property of L. camara is yet to be investigated. Therefore, the present study was undertaken to examine phyto- chemistry and in vitro α-amylase inhibitory potential of different solvent extracts of L. camara leaves collected from local areas of Serdang, Malaysia in July, 2015. The plant leaves were dried at room temperature and ground into powder. About 50 g of powdered leaves were extracted with 100 mL each of petroleum ether, chloroform, ethyl acetate, acetone, methanol and water and placed on orbital shaker at room temperature for 48 hours. After filtration, the filtrates were evaporated to dryness using rotavapor. The final yield of chloroform, ethyl acetate, acetone and methanol extracts were found to be 3.8%, 9.0%, 6.0% and 11.8% respectively. The phytocomponents present in each extract were deter- mined using standard protocols (Harborne, 1998; Kumara et al., 2012). Final concentrations of each extracts were prepared by dissolving in dimethyl sulfoxide and α-amylase inhibitory assay was perfor- med using the method of Tamil et al. (2010). The study employed acarbose as the standard inhibitor of α- amylase. Preliminary phytochemical analysis revealed the presence of phenols, flavonoids and cardiac glycosides in all the solvent extracts tested. However, saponins were absent in chloroform extract while, in ethyl acetate, acetone and methanol extracts tannins were not determined (Table I). In vitro α-amylase inhibitory potential of various solvent extracts (20-100 μg/mL) of L. camara leaves are shown in Figure 1. Acetone extracts A Journal of the Bangladesh Pharmacological Society (BDPS) Bangladesh J Pharmacol 2015; 10: 962-963 Journal homepage: www.banglajol.info Abstracted/indexed in Academic Search Complete, Agroforestry Abstracts, Asia Journals Online, Bangladesh Journals Online, Biological Abstracts, BIOSIS Previews, CAB Abstracts, Current Abstracts, Directory of Open Access Journals, EMBASE/Excerpta Medica, Google Scholar, HINARI (WHO), International Pharmaceutical Abstracts, Open J-gate, Science Citation Index Expanded, SCOPUS and Social Sciences Citation Index ISSN: 1991-0088; DOI: 10.3329/bjp.v10i4.25371 Letter to the Editor Table I Phytochemical of different extracts of L. camara leaves Chloro- form Ethyl acetate Acetone Metha- nol Phenols Present Present Present Present Flavonoids Present Present Present Present Saponins Absent Present Present Present Tannins Present Absent Absent Absent Cardiac glycosides Present Present Present Present Terpenoids Present Absent Absent Present Figure 1: In vitro α-amylase inhibitory potential of various solvent extracts of Lantana camara at 100 μg/mL concentration exhibited predominant α- amylase inhibitory potential (57.7 ± 1.5%) with an IC50 value of 66.8 μg/mL. The reference drug, acarbose inhibited 62.4 ± 0.4% of α-amylase activity at 100 μg/ mL concentration and its IC 50 value was found to be 80 μg/mL. As the concentration of all the extracts increased, the inhibitory potential was observed to increase representing a dose dependent α -amylase inhibitory activity. Ethyl acetate extract at 20 μg/mL concentration had the lowest inhibitory activity (8.66 ± 1.3%). On the other hand, chloroform, ethyl acetate and methanol extracts at 100 μg/mL exhibited 33 ± 1.5 %, 28 ± 1.5 % and 36.3 ±0.6 % respectively with IC50 value of >150 μg/mL. Acetone extracts of L. camara exhibited superior α-amylase inhibitory potential in comparison to standard drug, acarbose. Thus, this study indicates the possible exploration of L. camara in the development of new drug molecule to combat type 2 diabetes by inhibiting carbohydrate hydrolyzing enzymes. Mallappa Kumara Swamy and Uma Rani Sinniah Department of Crop Science, Faculty of Agriculture, Universiti Purta Malaysia, 43400 UPM Serdang, Selangor, Malaysia. Corresponding author: email: swamy.bio@gmail.com; Tel: +601-7248-4528 References Ganesh T, Sen S, Thilagam E, Thamotharan G, Loganathan T, Chakraborty R. Pharmacognostic and anti-hyperglycemic evaluation of Lantana camara (L.) var. aculeate leaves in alloxan-induced hyperglycemic rats. Int J Res Pharm Sci. 2010; 1: 247-52. Harborne JB. Phytochemical methods. London, Chapman and Hall Publication, 1998, pp 34-88. Kazmi I, Rahman M, Afzal M, Gupta G, Saleem S, Afzal O, Shaharyar MA, Nautiyal U, Ahmed S, Anwar F. Anti- diabetic potential of ursolic acid stearoyl glucoside: A new triterpenic gycosidic ester from Lantana camara. Fitoterapia 2012; 83: 142-46. Kumara SM, Sudipta KM, Lokesh P, Neeki A, Rashmi W, Bhaumik H, Darshil H, Vijay R, Kashyap SSN. 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