Bangladesh Journal of Pharmacology Volume: 12; Number 1; Year 2017 Cite this article as: Shah AU, Khan RA, Ahmed M. Pharmacological activities of methanolic crude extract of Quercus dialatata fruit. Bangladesh J Pharmacol. 2017; 12: 91-92. Pharmacological activities of the methano- lic extract of Quercus dialatata fruit Sir, Natural products have been in practice for the treatment of free radicals (Kokate and Purohit 2004). Reactive oxygen species are increased or in the body the anti-oxidants level decreased thus the balance changes towards the pro-oxidants and this conditions is known as oxidation stress and cellular damage occur in prolong oxidative stress. To solve this problem we can add the antioxidants in a proper amount in our nutrition (Ku and Mun, 2007). According to Rababah et al. (2004) different parts of the plants such as leaves, fruits, oil seed roots and vegetables have the capacity as natural antioxidants. Presently we arranged a study to investigate the methanolic fruit extract of Quercus dialatata a novel plant obtained from hilly areas for antifungal, antioxidant and phytochemical analysis. Dry fruit of Q. dialatata was collected from the North Waziristan Agency and the methanolic extract was prepared using rotary evaporator. Protocol of Duraipandiyan and Ignacimuthu (2009) was used to screen cytotoxic, DPPH and antifungal activities while Re et al. (1999) for ABTS and Nishikimi et al., (1972) for superoxide free radical scavenging activities. Results of the present study revealed that the crude extract showed that the death of brine shrimp was directly proportional to the concentration of fruit extract and was maximum (70.0%) at 1,000 µg/mL (Table I). Significant scavenging results were obtained during scavenging of free radicals viz; 71.2 ± 2.1% against DPPH, 76.3 ± 2.4% to ABTS and 30 ± 3.5% against superoxide at 600 μg/mL were obtained (Table II). Growth inhibition of 6.8 ± 0.2 and 4.7 ± 0.6 mm were recorded using Alternaria and Fusarium oxysporium (Figure 1). The results obtained in this study indicate that C. Dialatota possesses significant anti-oxidant, antifungal and cytotoxic actvaties which are may be due to the presence of bioactive compounds. Akram Ullah Shah, Rahmat Ali Khan and Mushtaq Ahmed Department of Biotechnology, University of Science and Technology, Bannu 28100, Pakistan. Corresponding author: email: Rahmatgul_81@yahoo.com References Duraipandiyan V, Ignacimuthu S. Antibacterial and antifungal activity of flindersine isolated from the traditional medicinal plant, Toddalia asiatica (L.) Lam. J Ethanopharmacol. 2009; A Journal of the Bangladesh Pharmacological Society (BDPS) Bangladesh J Pharmacol 2017; 12: 91-92 Journal homepage: www.banglajol.info Abstracted/indexed in Academic Search Complete, Agroforestry Abstracts, Asia Journals Online, Bangladesh Journals Online, Biological Abstracts, BIOSIS Previews, CAB Abstracts, Current Abstracts, Directory of Open Access Journals, EMBASE/Excerpta Medica, Google Scholar, HINARI (WHO), International Pharmaceutical Abstracts, Open J-gate, Science Citation Index Expanded, SCOPUS and Social Sciences Citation Index ISSN: 1991-0088; DOI: 10.3329/bjp.v12i1.30740 Letter to the Editor Table I Survival of brine shrimps in the presence of vari- ous concentration of Q. dialatata extract Concentration (µg/mL) No. of brine shrimp % Survival 50 10 80.0 ± 1.1 100 10 60.0 ± 1.3 300 10 50.2 ± 1.0 500 10 40.2 ± 1.4 1000 10 30.1 ± 1.7 Data are mean ± SD Alternaria Terbinafine Fusarium Terbinafine oxysporium 18 16 14 12 10 8 6 4 2 0 Figure 1: Antimicrobial activity of extract (mm) Zo ne o f i nh ib iti on (m m ) Table II Free radical scavenging activity Free Radical % Scavenging ability DPPH 71.2 ± 2.1 ABTS 76.3 ± 2.4 Superoxide radical 30.0 ± 3.5 Data are mean ± SD 123: 494-98. Kokate CK, Purohit AP. Text book of Pharmacognosy. 2004; 29: 317-18. Ku CB, Mun SP. Antioxidant activities of ethanol extracts from seeds in fresh Bokbunja (Rubus coreanus Miq) and wine processing waste. Bioresour Technol. 2007; 99: 4503-09. Nishikimi M, Rao NA, Yagi K. The occurance of superoxide anion in the reaction of reduced phenazine metho-sulphate and molecular oxygen. Biochem Biophys Res Commun. 1972; 46: 849-54. Rababah TM, Hettlarachchy NS, Horex R. Total phenolics and antioxidant activities of fenugreek, green tea, black tea, grape seed, ginger, rosemary, gotu kola and ginkgo extracts, vitamin E and tert. butylhydroquinone. J Agric Food Chem. 2004; 52: 5183-86. Re R, Pellegrino N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolonization assay. Free Radical Biol Med. 1999; 26; 1231-37. Bangladesh J Pharmacol 2017; 12: 91-92 92 Free Radical Scavenging ability: DatePrinted: This article was downloaded by you on: Mar 04, 2017