Bangladesh Journal of Pharmacology Volume: 17; Number 2; Year 2022 Cite this article as: Gaikwad RG, Shinde AJ. Antiproliferative effect of Memecylon malabaricum leaves methanolic extract against A-431 cell lines. Bangladesh J Pharmacol. 2022; 17: 54-55. Antiproliferative effect of Memecylon malabaricum leaves methanolic extract against A-431 cell lines Sir, Psoriasis is a chronic, immune-mediated inflammatory disorder of the skin. The pathogenesis includes the environmental trigger factors with genetic factors, trauma, chemicals, bacterial infection, etc. (Gaikwad et al., 2021). Various treatments are available for psoriasis management, but herbal therapy is accepted nowadays because of its popularity, minimum adverse effects, etc. Some studies suggest using natural in psoriasis treatment only because of their ability to inhibit proliferation. Literature reveals the antiproliferative effect of some plants such as Acacia nilotica (Thiagarajan et al. 2020), Glycyrrhiza glabra (Shinde et al., 2016), Euphorbia fischeriana (Wu at al., 2016), Triumfetta welwit- schii (Moyo and Mukanganyama, 2015), Xylopia aethio- pica (Adaramoye et al., 2011), etc. The antiproliferative effect of plant Memecylon edule had been described. Rutin, ursolic acid and thujone isola- ted from M. edule exhibited antiproliferative effect against U-937 and HT-60 cell lines (Srinivasan et al., 2016). Herein, the antiproliferative effect of methanolic extract of M. malabaricum was assessed against A-431 cell lines using the MTT assay method. In vitro antiproliferative activity was done with A-431 cell lines (Khatoon et al., 2021). In brief A-431 cells were incubated at a concentration of 1 × 104 cells/mL in a culture medium (24 hours at 37°C, 5% CO2). Cells were seeded at a concentration (70 μL) 104 cells/well in a 100 μL culture medium and 100 μL sample of M. malabaricum leaves extract in (50, 100, 150, 200, 250 μg/ mL) into microplates, respectively (tissue culture grade, and 96 wells). The control wells were incubated with DMSO (0.2% in PBS) and cell line. All samples were incubated in triplicate. Cell cultures were incubated for 24 hours at 37°C and 5% CO2 in a CO2 incubator (Thermo Scientific BB1505). After incubation, the medium was removed entirely and added 20 μL of MTT reagent (5 mg/min PBS). After the addition of MTT, cells were incubated for 4 hours at 37°C in a CO2 incubator. The yellowish MTT was reduced to dark- colored formazan by viable cells only after removing the medium altogether. Added 200 μL of DMSO (kept for 10 min) and incubated at 37°C (wrapped with aluminium foil). Five concentrations of the M. malabaricum leaves extract were analyzed by measuring the absorbance of each sample by a microplate reader (Benesphera E21) at a wavelength of 550 nm. As shown in Figure 1, the methanolic leaves extract of M. malabaricum showed remarkable antiproliferative activity against the A-431 cell line with %cell growth inhibition value of 20.6–35.7. The antiproliferative activity of the methanolic leaves extracts of M. malabaricum was comparable to the standard 5- fluorouracil (5-FU). Antiproliferative activity of the leaves extracts of M. malabaricum indicated the potential of the M. malabaricum for antipsoriatic potential. The M. malabaricum showed dose-dependent antiproliferative activity. Epidermal proliferation occurs due to an increase in cGMP and decreased cAMP because of lipid peroxidation (Nowak-Perlak et al., 2022). The antiproliferative effects result from gene expression regulation and are initiated with transcriptional activation of JAK-STAT, signal transduction pathways (Aalinkeel et al., 2010) The authors are thankful to the Principal, Dr. H. N. More Bharati Vidyapeeth College of Pharmacy, for providing A Journal of the Bangladesh Pharmacological Society (BDPS) Bangladesh J Pharmacol 2022; 17: 54-55 Journal homepage: www.banglajol.info; www.bdpsjournal.org Abstracted/indexed in Academic Search Complete, Agroforestry Abstracts, Asia Journals Online, Bangladesh Journals Online, Biological Abstracts, BIOSIS Previews, CAB Abstracts, Current Abstracts, Directory of Open Access Journals, EMBASE/Excerpta Medica, Global Health, Google Scholar, HINARI (WHO), International Pharmaceutical Abstracts, Open J-gate, Science Citation Index Expanded, SCOPUS and Social Sciences Citation Index ISSN: 1991-0088; DOI: 10.3329/bjp.v17i2.59408 Letter to the Editor This work is licensed under a Creative Commons Attribution 4.0 International License. You are free to copy, distribute and perform the work. You must attribute the work in the manner specified by the author or licensor Figure 1: Antiproliferative activity of methanolic extract Me- mecylon malabaricum leaves % I n h ib it io n 100 80 60 40 20 0 100 200 300 400 500 Concentration (µg/mL) 5-Fluorouracil M. malabarium https://www.tandfonline.com/author/Srinivasan%2C+R facilities for research work. The authors are also grateful to Chhatrapati Shahu Maharaj Research, Training and Human Development Institute, Pune, for providing financial assistance in this work. Ravindra G. Gaikwad and Anilkumar J. Shinde Department of Pharmaceutics, Bharati Vidyapeeth College of Pharmacy, Kolhapur, India. Corresponding author: Email: anilkumar.shinde@bharatividyapeeth.edu References Aalinkeel R, Hu Z, Nair BB, Sykes DE, Reynolds JL, Mahajan SD, Schwartz SA. Genomic analysis highlights the role of the JAK-STAT signaling in the antiproliferative effects of dietary flavonoid: Ashwagandha in prostate cancer cells. 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Screening and analysis of anti-A431 cell proliferative fraction of Euphorbia fischeriana Steud. extract. Biomed Res. 2016; 27: 1231-36. Bangladesh J Pharmacol 2022; 17: 54-55 55 mailto:Anilkumar.shinde@bharatividyapeeth.edu