Microsoft Word - 18. BJPT 15 - 155_edit KA. REV.doc Bangladesh J. Plant Taxon. 23(2): 247-253, 2016 (December) © 2016 Bangladesh Association of Plant Taxonomists PALYNOLOGICAL STUDIES ON SOME SPECIES OF ANABASIS L. (AMARANTHACEAE) FROM IRAN M. ASSADI, S.M.M. HAMDI1 AND F. FAJANI2 Research Institute Rangelands and Forests, Tehran, Iran Keywords: Amaranthaceae; Anabasis; Exine ornamentation; Palynology; Iran. Abstract The paper presents pollen micromorphology by scanning electron microscopy of seven Anabasis L. species namely, A. haussknechtii Bge., A. aphylla L., A. calcarea (Charif & Allen) Bokhari & Wendelbo, A. eugeniae Iljin, A. eriopoda (Shrernk) Volkens, A. annua Bge. and A. setifera Moq. from Iran. Pollen grains in all studied species are peripolyporate, spherical and are of two basic types based on pores diameter inner of holes. The study showed that the sculpturing of exine provides valuable characters in species delimitation, sometimes even for closely related ones. An artificial key based on pollen characters for seven species is provided. Introduction Amaranthaceae comprises of approximately 163 genera and 1825 species (Kadereit et al., 2003 and APG III, 2009). Anabasis belonging to the tribe Salsoleae s.l. is one of the largest tribes in Amaranthaceae, includes one-third of the genera currently recognized in the family (Ku¨hn et al., 1993), distributed throughout arid, semiarid, saline, and hypersaline ecosystems of temperate and subtropical regions (Pyankov, et al., 2001; Kadereit et al., 2003 and Akhani et al., 2007). Anabasis is distributed in Iran by 10 species and 2 varieties (Freitag, 1997 and Assadi, 2001). Iran is one of the distribution centers for Anabasis (Akhani et al., 2007). Tsukada (1967) studied amranth-chenopoid pollens by electron microscope and used pollen characters like pores, shape, size and conical tubercle as key characters. There is no study on the pollen micromorpholgy on Anabasis from Iran. The present study aims to investigate the pollen micromorpholgy of Anabasis, to evaluate its taxonomic value and to prepare an identification key for seven Anabasis species from Iran based on pollen micromorphological characters. Materials and Methods This study includes seven Anabasis species from Iran based on materials deposited in the National Herbarium of Iran, Research Institute of Forests and Rangelands (TARI) and Islamic Azad University Garmsar Herbarium (IAUGH) and also collected from different localities of Iran during 2013-2014 (Table 1). Pollen grains of 7 species of the genus Anabasis were studied by scanning electron microscope. Samples were obtained mostly from fresh collected herbarium specimens. The voucher and the pollen specimens, deposited in TARI Herbarium, are listed under table 1. For SEM, were used the protocol explained by Davies (1999) with some modifications. The specimens were mounted on 12.5 mm diameter stubs and attached with sticky tabs and then coated in a sputter coater with approximately 25 µm of Gold- Paladium. Pollens were examined and photographed by a Philips scanning electron microscope model XL. Following quantitative                                                              1 Central Tehran Branch, Islamic Azad University, Tehran, Iran. Corresponding author. Email. m.hamdi@iauctb.ac.ir 2 Garmsar Branch, Islamic Azad University, Garmsar, Iran. 248 ASSADI et al. parameters were recorded: polar and equatorial pollen, P/E, pore diameters inner and outer, number of conical tubercle, pore high, distance between of centers of the adjacent pores and pores number. The terminology used for describing the pollens features followed in general by Moore et al. (1991) and Punt et al. (1994). Table 1. Distribution of species of Anabasis L. studied. Species Locality Collector Altitude (m) Herbarium number Herbarium name Anabasis setifera Moq. Tehran-Eyvaneky, Roude Shour Fajani 780 5899 IAUGH A. annua Bge Kerman, 45 km to Mahan, Assadi & Amirabadi 2300 66521 TARI A. aphylla L. Tehran, 31 km south of Firouzkouh, Pirdeh Fajani 2200 5900 IAUGH A. haussknechtii Bge. Semnan, 10 km, S.-E. Semnan, near Aella village Fajani 1050 5901 IAUGH A. eugeniae Iljin Azerbaijan, 34 km from Alamdar toward Khodaafarin, between Ahmadabad and Siahroud Assadi and Shahsavari 700 65828 TARI A. eriopoda (Shrenk) Volkens Protected of Touran area, west- nourth of Dochah, in road of Chah Jaam Feritag 1150 14103 TARI A. calcarea (Charif&Aellen) Bokhari and Wendelbo Semnan, 25 km east-south of Masnan in road of Taroud Wendelboo and Foroughi 1120 18684 TARI Results The main features of the investigated of pollen are summarized in Table 2. Our studies show that the sculpturing of exine provides valuable characters for separating the species, sometimes even for closely related ones, and delimitation of species. Pollen grains of all studied species are peripolyporate, spherical, having 32-83 conical tubercles on pore of pollen surface, polar length 12.4-26.10 µm, equatorial length 9.83-25 µm, number of pores 8-25 on pollen surface, 23-85 conical tubercle per 5 µm, distance between the center of adjacent pores 4.84-10 µm, pores diameter (outer) 3.5-10.2 µm, pores diameter (inner) 2-6 µm, pores height 0.1-0.35 µm and tectal of conical tubercle length 0.2-0.6 µm. Following Punt et al. (1994) two basic types of pollen grains are distinguished based on holes pore diameters (inner), such as, Type A: pore diameter of holes (inner) on the exine is less than 3 µm² and Type B: pore diameter of holes (inner) on the exine is more than 3 µm (Table 2). Type A includes A. eriopoda, A. haussknechtii, A. annua and A. aphylla; and Type B includes A.eugeniae, A. calcarea and A. setifera. On the basis of the exine sculpturing at proximal face, two main pollen types, as faveat tectum and faweat tectum are recognized (Table 2, Figs. 1& 2). PALYNOLOGICAL STUDIES ON SOME SPECIES OF ANABASIS 249 250 ASSADI et al. Fig. 1. Micrograph of pollen grains in Anabasis (Amaranthaceae). A-B, faveat tectum at the proximal face in A. setifera pollen with faveat ornamentation of exine. C-D, faweat tectum at the proximal face in A. annua pollen with faveat ornamentation of exine. E-F, faweat tectum at the proximal face in A. eriopoda pollen with faveat ornamentation of exine. G-H, faweat tectum at the proximal face in A.eugenia. pollen with faveat ornamentation of exine. Scale bars: A, C, E & G= 10 µm; B, D, F, H = 1 µm. PALYNOLOGICAL STUDIES ON SOME SPECIES OF ANABASIS 251 Fig. 2. Micrograph of pollen grain in Anabasis (Amaranthaceae). I-J. Faveat tectum at the proximal face in A. calcarea pollen with faveat ornamentation of exine. K-L. Faweat tectum at the proximal face in A. aphylla pollen with faveat ornamentation of exine. M-N. Faweat tectum at the proximal face in A. hausskanchtii pollen with faveat ornamentation of exine. Scale bars: I, K & M = 10 µm; J, L & N = 1 µm. Discussion The basic palynomorphological characters of Anabasis in all studied species are the occurrence of spherical shape and peripolyporate pollens. The importance of pore number and C/D ratio in the Chenopodium L. species was emphasised by Andrews & Swanson (1967) and Uotila (1997). The present study does not show any correlation between pore number and pollen size. 252 ASSADI et al. The present study confirms the findings of Pinar & Inceoglu (1999) and Hamdi et al. (2009) that the pollen morphology of the Salsola L. and Chenopodium species in general shows uniform type characteristics by pollen micromorphology. Pollen grains in the Anabasis can be grouped into two groups as found in Amaranthaceae by Tsukada (1967). Pollen morphological characters of the Anabasis species are closely related to Salsola, Suaeda Forssk. Ex Scop., Chenopodium, Halocharis Moq. , Sarcobatus Nees , Traganum Delile of Amaranthaceae, as well as to the pollen morphology type found in Portulacaceae, Phytolacaceae and Caryophyllaceae (Tsukada, 1967; Uotila, 1974; Skvarla & Nowicke, 1976), Youngjae & Lee, 1995; Borsch, 1998); Pinar & Inceoglu, 1999; Hamdi et al., 2009 and Nikolaevna Toderich et al., 2010). There is variation in distance between the centers of the adjacent pores and corresponding pore number (Mc Andrews & Swanson, 1967). Nikolaevna Toderich (2010) noted the correlation between length of the polar and equatorial axes and size of the flower in Salsola. This study suggests that there are significant differences in pollen size, tubercles on surface exine, distance between the centers of the adjacent pores and pore numbers on surface exine within Anabasis species. The results of this study show that pollen micromorphology can be used in delimiting species of this genus. Based on pollen morphological characters following key is provided in indentifying seven species in Anabasis from Iran Key to Iranian Anabasis species based on pollen characters 1. Pore diameter (inner) of holes on the exine less than 3 µm 2 - Pore diameter (inner) of holes on the exine more than 3 µm 5 2. Pore diameter (outer) of holes on the exine less than 4 µm A. haussknechtii - Pores diameter (outer) of holes on the exine more than 4 µm 3 3. Pore height less than 0.3 µm A. eriopoda - Pore height more than 0.3 µm 4 4. Number of conical tubercle on holes 80-83 A. annua - Number of conical tubercle on holes 32-35 A. aphylla 5. 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