Wedelia trilobata (L Bangladesh J. Plant Taxon. 17(1): 93-96, 2010 (June) - Short communication © 2010 Bangladesh Association of Plant Taxonomists A COMPARISON OF POLLEN GRAINS OF POTENTILLA RECTA L. (ROSACEAE) GROUPS A, B & C IN TURKEY ELA AYŞE KÖKSAL1, BARIŞ AŞCI2 AND NUR MÜNEVVER PINAR2,3 Elementary Science Education Department, Faculty of Education, Derbent Campus, Niğde University, 51100 Niğde, Turkey Keywords: Pollen grains; Potentilla recta L.; Rosaceae. Potentilla L. (Rosaceae) is represented by 59 species in Turkey (Tübitak, 2005). It is, however, difficult to identify some of its members at the species and subspecies levels and P. recta L. is one of them. This species is an extremely variable polyploid and includes various samples resembling morphologically and linking to each other. Peşmen (1972) grouped P. recta into A, B & C based upon some differences in sepal, petal and leaf anatomy. In the present study the pollen grains of P. recta groups A, B & C were examined and compared using light microscope (LM), scanning electron microscope (SEM) and transmission electron microscope (TEM) to elucidate their taxonomic position at species or subspecies level. The Wodehouse (1935, W) and Erdtman (1960, Acetolysis, A) methods were used to prepare slides of pollen grains for LM study where different pollen measurements were recorded under a Leitz-Wetzlar microscope (×16; ×100). The measurements of each character were taken until a Gauss Curve was obtained. In addition, at least 30 pollen grains from each group were measured for 1) the length of exine and intine from the slides prepared with the W method and 2) the length of exine from the slides prepared with the A method. The photographs were taken with an Olympus C-35 AD-4 type camera attached on an Olympus BH 2 microscope. For the SEM study, the unacetolysed pollen grains were directly placed on stubs, covered with gold and their photographs were taken with a JSM electron microscope. Skvarla (1966) was followed to examine the acetolysed pollen grains under TEM. In the TEM micrographs, the tectum, columella, foot layer, and endexine were measured at least 5 different points and their means were taken. The major pollen grain features of groups A, B & C of P. recta are given in Tables 1 and 2 and depicted in Figs. 1-3. In all groups, pollen grains are monad, isopolar, radially symmetric and tricolporate. Although there are variations among the groups according to the pollen shape (Table 2), but they are roughly prolate. The amb shape is inter semiangular. The ornamentation is suprastriate-microperforate. The liras lie parallel to the colpus. The exine is subtectate. The intratectal columellae that form tectum are distinct, small, sometimes bifurcated and thin. The infratectal columellae are distinct, long, bifurcated. There are also foramina on the columella. The foot layer is not continuous. 1 E-mail: eakoksal@nigde.edu.tr; elaaysekoksal@gmail.com 2 Biology Department, Faculty of Science, Ankara University, Ankara, 06100 Turkey 3 Corresponding author. E-mail: pinar@science.ankara.edu.tr 94 KÖKSAL et al. 94 KÖKSAL et al. POLLEN GRAINS OF POTENTILLA RECTA 95 Fig. 1. LM (A-E) and SEM (F-G) micrographs of Potentilla recta Group A pollen grains. A. Pore (W); B. Amb shape in polar view (W); C. Pore in equatorial view (A); D. Colpus in equatorial view (A); E. Exine structure and aperture in polar view (A) (Bar = 10 µm); F. Equatorial view, ornamentation and aperture; G. Ornamentation in polar axis. Fig. 2. LM (A-E), SEM (F) and TEM (G, H) micrographs of Potentilla recta Group B pollen grains. A, B. Exine structure and aperture in equatorial view (W); C. Amb shape in polar view (W); D, E. Exine structure and aperture in equatorial view (A) (Bar = 10 µm); F. Microperforate ornamentation in polar axis; G. General structure of exine structure in a section from the aperture region; H. Exine structure in a section from mesocolpium area (arrows show that exine structure is subtectate). 96 KÖKSAL et al. Fig. 3. LM (A-D) and SEM (E) micrographs of Potentilla recta Group C pollen grains. A, B. Exine structure and aperture in equatorial view (A); C, D. Exine structure, aperture and amb shape in polar view (A) (Bar = 10 µm); E. Ornamentation in mesocolpium. The endexine is discontinuous. It is thicker under the aperture. The edge of the colpus is straight and the colpus ends are acute. The operculum is present. The ornamentation is striate. The pore is lalongate. Based on the data on the pollen grains, all the three groups seem to be similar in regard of the morphological features, thus cannot be classified into separate taxa. Acknowledgements We would like to thank Professor Emeritus Dr. Özden İnceoğlu for her initial guidance during the course of this study. References Erdtman, G. 1960. The Acetolysis Method. A revised description. Svensk Botanisk Tidskrift 54: 561-564. Peşmen, H. 1972. Potentilla recta L. Flora of Turkey. Vol. 4. Edinburgh University Press, Edinburgh. pp. 41- 45. Skvarla, J.J. 1966. Techniques of pollen and spore electron microscopy. I. Staining, dehydration and embedding. Oklahoma Geology Notes 26: 179-186. Tübitak. 2005. Taxonomic species database of Turkey Vol. 10. Wodehouse, R.P. 1935. Pollen Grains. McGraw Hill, New York. pp. 1-435. (Manuscript received on 29 January 2009; revised on 20 July 2009)