id	sid	tid	token	lemma	pos
bionys-354	1	1	jovanović	jovanović	INTJ
bionys-354	1	2	et	et	PROPN
bionys-354	1	3	al	al	PROPN
bionys-354	1	4	2020	2020	NUM
bionys-354	1	5	,	,	PUNCT
bionys-354	1	6	biologica	biologica	PROPN
bionys-354	1	7	nyssana	nyssana	PROPN
bionys-354	1	8	11(2	11(2	PROPN
bionys-354	1	9	)	)	PUNCT
bionys-354	1	10	11	11	NUM
bionys-354	1	11	(	(	PUNCT
bionys-354	1	12	2	2	NUM
bionys-354	1	13	)	)	PUNCT
bionys-354	1	14	december	december	PROPN
bionys-354	1	15	2020	2020	NUM
bionys-354	1	16	:	:	PUNCT
bionys-354	1	17	139	139	NUM
bionys-354	1	18	-	-	SYM
bionys-354	1	19	147	147	NUM
bionys-354	1	20	doi	doi	NOUN
bionys-354	1	21	:	:	PUNCT
bionys-354	1	22	10.5281	10.5281	NUM
bionys-354	1	23	/	/	SYM
bionys-354	1	24	zenodo.4393973	zenodo.4393973	NOUN
bionys-354	1	25	optimizing	optimizing	NOUN
bionys-354	1	26	conditions	condition	NOUN
bionys-354	1	27	for	for	ADP
bionys-354	1	28	mgmt	mgmt	NOUN
bionys-354	1	29	promoter	promoter	NOUN
bionys-354	1	30	methylation	methylation	NOUN
bionys-354	1	31	status	status	NOUN
bionys-354	1	32	analysis	analysis	NOUN
bionys-354	1	33	in	in	ADP
bionys-354	1	34	glioblastoma	glioblastoma	NOUN
bionys-354	1	35	ffpe	ffpe	NOUN
bionys-354	1	36	samples	sample	VERB
bionys-354	1	37	original	original	ADJ
bionys-354	1	38	article	article	NOUN
bionys-354	1	39	nikola	nikola	PROPN
bionys-354	1	40	m.	m.	PROPN
bionys-354	1	41	jovanović	jovanović	PROPN
bionys-354	1	42	university	university	PROPN
bionys-354	1	43	of	of	ADP
bionys-354	1	44	niš	niš	PROPN
bionys-354	1	45	,	,	PUNCT
bionys-354	1	46	faculty	faculty	NOUN
bionys-354	1	47	of	of	ADP
bionys-354	1	48	sciences	science	NOUN
bionys-354	1	49	and	and	CCONJ
bionys-354	1	50	mathematics	mathematic	NOUN
bionys-354	1	51	,	,	PUNCT
bionys-354	1	52	department	department	NOUN
bionys-354	1	53	of	of	ADP
bionys-354	1	54	biology	biology	NOUN
bionys-354	1	55	and	and	CCONJ
bionys-354	1	56	ecology	ecology	NOUN
bionys-354	1	57	,	,	PUNCT
bionys-354	1	58	višegradska	višegradska	PROPN
bionys-354	1	59	33	33	NUM
bionys-354	1	60	,	,	PUNCT
bionys-354	1	61	18000	18000	NUM
bionys-354	1	62	niš	niš	NOUN
bionys-354	1	63	,	,	PUNCT
bionys-354	1	64	serbia	serbia	PROPN
bionys-354	1	65	nikolajov90@gmail.com	nikolajov90@gmail.com	X
bionys-354	1	66	(	(	PUNCT
bionys-354	1	67	corresponding	corresponding	ADJ
bionys-354	1	68	autor	autor	NOUN
bionys-354	1	69	)	)	PUNCT
bionys-354	1	70	vesna	vesna	PROPN
bionys-354	1	71	nikolov	nikolov	PROPN
bionys-354	1	72	university	university	PROPN
bionys-354	1	73	of	of	ADP
bionys-354	1	74	niš	niš	PROPN
bionys-354	1	75	,	,	PUNCT
bionys-354	1	76	faculty	faculty	NOUN
bionys-354	1	77	of	of	ADP
bionys-354	1	78	medicine	medicine	NOUN
bionys-354	1	79	,	,	PUNCT
bionys-354	1	80	clinic	clinic	NOUN
bionys-354	1	81	of	of	ADP
bionys-354	1	82	neurosurgery	neurosurgery	NOUN
bionys-354	1	83	,	,	PUNCT
bionys-354	1	84	clinical	clinical	ADJ
bionys-354	1	85	center	center	NOUN
bionys-354	1	86	,	,	PUNCT
bionys-354	1	87	18000	18000	NUM
bionys-354	1	88	niš	niš	NOUN
bionys-354	1	89	,	,	PUNCT
bionys-354	1	90	serbia	serbia	PROPN
bionys-354	1	91	v.novak@yahoo.com	v.novak@yahoo.com	PROPN
bionys-354	1	92	nataša	nataša	PROPN
bionys-354	1	93	vidović	vidović	PROPN
bionys-354	1	94	university	university	PROPN
bionys-354	1	95	of	of	ADP
bionys-354	1	96	niš	niš	PROPN
bionys-354	1	97	,	,	PUNCT
bionys-354	1	98	faculty	faculty	NOUN
bionys-354	1	99	of	of	ADP
bionys-354	1	100	medicine	medicine	NOUN
bionys-354	1	101	,	,	PUNCT
bionys-354	1	102	pathology	pathology	NOUN
bionys-354	1	103	and	and	CCONJ
bionys-354	1	104	pathological	pathological	ADJ
bionys-354	1	105	anatomy	anatomy	NOUN
bionys-354	1	106	center	center	NOUN
bionys-354	1	107	,	,	PUNCT
bionys-354	1	108	18000	18000	NUM
bionys-354	1	109	niš	niš	NOUN
bionys-354	1	110	,	,	PUNCT
bionys-354	1	111	serbia	serbia	PROPN
bionys-354	1	112	vidovic.patologija@gmail.com	vidovic.patologija@gmail.com	PROPN
bionys-354	1	113	jelena	jelena	PROPN
bionys-354	1	114	vitorović	vitorović	PROPN
bionys-354	1	115	university	university	PROPN
bionys-354	1	116	of	of	ADP
bionys-354	1	117	niš	niš	PROPN
bionys-354	1	118	,	,	PUNCT
bionys-354	1	119	faculty	faculty	NOUN
bionys-354	1	120	of	of	ADP
bionys-354	1	121	sciences	science	NOUN
bionys-354	1	122	and	and	CCONJ
bionys-354	1	123	mathematics	mathematic	NOUN
bionys-354	1	124	,	,	PUNCT
bionys-354	1	125	department	department	NOUN
bionys-354	1	126	of	of	ADP
bionys-354	1	127	biology	biology	NOUN
bionys-354	1	128	and	and	CCONJ
bionys-354	1	129	ecology	ecology	NOUN
bionys-354	1	130	,	,	PUNCT
bionys-354	1	131	višegradska	višegradska	PROPN
bionys-354	1	132	33	33	NUM
bionys-354	1	133	,	,	PUNCT
bionys-354	1	134	18000	18000	NUM
bionys-354	1	135	niš	niš	NOUN
bionys-354	1	136	,	,	PUNCT
bionys-354	1	137	serbia	serbia	PROPN
bionys-354	1	138	jelena.rajkovic@gmail.com	jelena.rajkovic@gmail.com	PROPN
bionys-354	1	139	svetlana	svetlana	PROPN
bionys-354	1	140	tošić	tošić	PROPN
bionys-354	1	141	university	university	PROPN
bionys-354	1	142	of	of	ADP
bionys-354	1	143	niš	niš	PROPN
bionys-354	1	144	,	,	PUNCT
bionys-354	1	145	faculty	faculty	NOUN
bionys-354	1	146	of	of	ADP
bionys-354	1	147	sciences	science	NOUN
bionys-354	1	148	and	and	CCONJ
bionys-354	1	149	mathematics	mathematic	NOUN
bionys-354	1	150	,	,	PUNCT
bionys-354	1	151	department	department	NOUN
bionys-354	1	152	of	of	ADP
bionys-354	1	153	biology	biology	NOUN
bionys-354	1	154	and	and	CCONJ
bionys-354	1	155	ecology	ecology	NOUN
bionys-354	1	156	,	,	PUNCT
bionys-354	1	157	višegradska	višegradska	PROPN
bionys-354	1	158	33	33	NUM
bionys-354	1	159	,	,	PUNCT
bionys-354	1	160	18000	18000	NUM
bionys-354	1	161	niš	niš	NOUN
bionys-354	1	162	,	,	PUNCT
bionys-354	1	163	serbia	serbia	PROPN
bionys-354	1	164	tosicsvetlana59@yahoo.com	tosicsvetlana59@yahoo.com	X
bionys-354	1	165	vladimir	vladimir	PROPN
bionys-354	1	166	j.	j.	PROPN
bionys-354	1	167	cvetković	cvetković	PROPN
bionys-354	1	168	university	university	PROPN
bionys-354	1	169	of	of	ADP
bionys-354	1	170	niš	niš	PROPN
bionys-354	1	171	,	,	PUNCT
bionys-354	1	172	faculty	faculty	NOUN
bionys-354	1	173	of	of	ADP
bionys-354	1	174	sciences	science	NOUN
bionys-354	1	175	and	and	CCONJ
bionys-354	1	176	mathematics	mathematic	NOUN
bionys-354	1	177	,	,	PUNCT
bionys-354	1	178	department	department	NOUN
bionys-354	1	179	of	of	ADP
bionys-354	1	180	biology	biology	NOUN
bionys-354	1	181	and	and	CCONJ
bionys-354	1	182	ecology	ecology	NOUN
bionys-354	1	183	,	,	PUNCT
bionys-354	1	184	višegradska	višegradska	PROPN
bionys-354	1	185	33	33	NUM
bionys-354	1	186	,	,	PUNCT
bionys-354	1	187	18000	18000	NUM
bionys-354	1	188	niš	niš	NOUN
bionys-354	1	189	,	,	PUNCT
bionys-354	1	190	serbia	serbia	PROPN
bionys-354	1	191	biovlada@yahoo.com	biovlada@yahoo.com	X
bionys-354	2	1	tatjana	tatjana	PROPN
bionys-354	2	2	mitrović	mitrović	VERB
bionys-354	2	3	university	university	PROPN
bionys-354	2	4	of	of	ADP
bionys-354	2	5	niš	niš	PROPN
bionys-354	2	6	,	,	PUNCT
bionys-354	2	7	faculty	faculty	NOUN
bionys-354	2	8	of	of	ADP
bionys-354	2	9	sciences	science	NOUN
bionys-354	2	10	and	and	CCONJ
bionys-354	2	11	mathematics	mathematic	NOUN
bionys-354	2	12	,	,	PUNCT
bionys-354	2	13	department	department	NOUN
bionys-354	2	14	of	of	ADP
bionys-354	2	15	biology	biology	NOUN
bionys-354	2	16	and	and	CCONJ
bionys-354	2	17	ecology	ecology	NOUN
bionys-354	2	18	,	,	PUNCT
bionys-354	2	19	višegradska	višegradska	PROPN
bionys-354	2	20	33	33	NUM
bionys-354	2	21	,	,	PUNCT
bionys-354	2	22	18000	18000	NUM
bionys-354	2	23	niš	niš	NOUN
bionys-354	2	24	,	,	PUNCT
bionys-354	2	25	serbia	serbia	PROPN
bionys-354	2	26	tatjanamitrovic3@gmail.com	tatjanamitrovic3@gmail.com	X
bionys-354	3	1	tatjana	tatjana	PROPN
bionys-354	3	2	jevtović	jevtović	PROPN
bionys-354	3	3	-	-	PUNCT
bionys-354	3	4	stoimenov	stoimenov	PROPN
bionys-354	3	5	university	university	PROPN
bionys-354	3	6	of	of	ADP
bionys-354	3	7	niš	niš	PROPN
bionys-354	3	8	,	,	PUNCT
bionys-354	3	9	faculty	faculty	NOUN
bionys-354	3	10	of	of	ADP
bionys-354	3	11	medicine	medicine	NOUN
bionys-354	3	12	,	,	PUNCT
bionys-354	3	13	department	department	NOUN
bionys-354	3	14	of	of	ADP
bionys-354	3	15	biochemistry	biochemistry	NOUN
bionys-354	3	16	,	,	PUNCT
bionys-354	3	17	18000	18000	NUM
bionys-354	3	18	niš	niš	NOUN
bionys-354	3	19	,	,	PUNCT
bionys-354	3	20	serbia	serbia	PROPN
bionys-354	3	21	tjevtovic@yahoo.com	tjevtovic@yahoo.com	PROPN
bionys-354	3	22	received	receive	VERB
bionys-354	3	23	:	:	PUNCT
bionys-354	3	24	may	may	AUX
bionys-354	3	25	14	14	NUM
bionys-354	3	26	,	,	PUNCT
bionys-354	3	27	2020	2020	NUM
bionys-354	3	28	revised	revise	VERB
bionys-354	3	29	:	:	PUNCT
bionys-354	3	30	september	september	PROPN
bionys-354	3	31	15	15	NUM
bionys-354	3	32	,	,	PUNCT
bionys-354	3	33	2020	2020	NUM
bionys-354	3	34	accepted	accept	VERB
bionys-354	3	35	:	:	PUNCT
bionys-354	3	36	november	november	PROPN
bionys-354	3	37	30	30	NUM
bionys-354	3	38	,	,	PUNCT
bionys-354	3	39	2020	2020	NUM
bionys-354	3	40	abstract	abstract	NOUN
bionys-354	3	41	:	:	PUNCT
bionys-354	3	42	the	the	DET
bionys-354	3	43	methylation	methylation	NOUN
bionys-354	3	44	status	status	NOUN
bionys-354	3	45	of	of	ADP
bionys-354	3	46	the	the	DET
bionys-354	3	47	mgmt	mgmt	NOUN
bionys-354	3	48	promoter	promoter	NOUN
bionys-354	3	49	represents	represent	VERB
bionys-354	3	50	the	the	DET
bionys-354	3	51	valuable	valuable	ADJ
bionys-354	3	52	prognostic	prognostic	ADJ
bionys-354	3	53	and	and	CCONJ
bionys-354	3	54	predictive	predictive	ADJ
bionys-354	3	55	marker	marker	NOUN
bionys-354	3	56	in	in	ADP
bionys-354	3	57	glioblastoma	glioblastoma	NOUN
bionys-354	3	58	(	(	PUNCT
bionys-354	3	59	gbm	gbm	NOUN
bionys-354	3	60	)	)	PUNCT
bionys-354	3	61	patients	patient	NOUN
bionys-354	3	62	undergoing	undergo	VERB
bionys-354	3	63	treatment	treatment	NOUN
bionys-354	3	64	with	with	ADP
bionys-354	3	65	alkylating	alkylate	VERB
bionys-354	3	66	agents	agent	NOUN
bionys-354	3	67	such	such	ADJ
bionys-354	3	68	as	as	ADP
bionys-354	3	69	temozolomide	temozolomide	NOUN
bionys-354	3	70	.	.	PUNCT
bionys-354	4	1	although	although	SCONJ
bionys-354	4	2	formalinfixed	formalinfixed	ADJ
bionys-354	4	3	and	and	CCONJ
bionys-354	4	4	paraffin	paraffin	NOUN
bionys-354	4	5	-	-	PUNCT
bionys-354	4	6	embedded	embed	VERB
bionys-354	4	7	tissue	tissue	NOUN
bionys-354	4	8	(	(	PUNCT
bionys-354	4	9	ffpe	ffpe	NOUN
bionys-354	4	10	)	)	PUNCT
bionys-354	4	11	signifies	signify	VERB
bionys-354	4	12	the	the	DET
bionys-354	4	13	most	most	ADV
bionys-354	4	14	commonly	commonly	ADV
bionys-354	4	15	used	use	VERB
bionys-354	4	16	source	source	NOUN
bionys-354	4	17	for	for	ADP
bionys-354	4	18	tissue	tissue	NOUN
bionys-354	4	19	-	-	PUNCT
bionys-354	4	20	based	base	VERB
bionys-354	4	21	molecular	molecular	ADJ
bionys-354	4	22	testing	testing	NOUN
bionys-354	4	23	,	,	PUNCT
bionys-354	4	24	its	its	PRON
bionys-354	4	25	use	use	NOUN
bionys-354	4	26	in	in	ADP
bionys-354	4	27	methylation	methylation	NOUN
bionys-354	4	28	-	-	PUNCT
bionys-354	4	29	specific	specific	ADJ
bionys-354	4	30	polymerase	polymerase	NOUN
bionys-354	4	31	chain	chain	NOUN
bionys-354	4	32	reaction	reaction	NOUN
bionys-354	4	33	(	(	PUNCT
bionys-354	4	34	msp	msp	PROPN
bionys-354	4	35	)	)	PUNCT
bionys-354	4	36	analysis	analysis	NOUN
bionys-354	4	37	manifests	manifest	VERB
bionys-354	4	38	certain	certain	ADJ
bionys-354	4	39	limitations	limitation	NOUN
bionys-354	4	40	due	due	ADP
bionys-354	4	41	to	to	ADP
bionys-354	4	42	low	low	ADJ
bionys-354	4	43	dna	dna	PROPN
bionys-354	4	44	integrity	integrity	NOUN
bionys-354	4	45	.	.	PUNCT
bionys-354	5	1	our	our	PRON
bionys-354	5	2	study	study	NOUN
bionys-354	5	3	aimed	aim	VERB
bionys-354	5	4	to	to	PART
bionys-354	5	5	identify	identify	VERB
bionys-354	5	6	the	the	DET
bionys-354	5	7	optimal	optimal	ADJ
bionys-354	5	8	mgmt	mgmt	PROPN
bionys-354	5	9	promoter	promoter	NOUN
bionys-354	5	10	msp	msp	PROPN
bionys-354	5	11	reaction	reaction	NOUN
bionys-354	5	12	conditions	condition	NOUN
bionys-354	5	13	concerning	concern	VERB
bionys-354	5	14	the	the	DET
bionys-354	5	15	utilization	utilization	NOUN
bionys-354	5	16	of	of	ADP
bionys-354	5	17	bisulfite	bisulfite	NOUN
bionys-354	5	18	-	-	PUNCT
bionys-354	5	19	converted	convert	VERB
bionys-354	5	20	ffpe	ffpe	NOUN
bionys-354	5	21	-	-	PUNCT
bionys-354	5	22	derived	derive	VERB
bionys-354	5	23	template	template	NOUN
bionys-354	5	24	dna	dna	NOUN
bionys-354	5	25	.	.	PUNCT
bionys-354	6	1	several	several	ADJ
bionys-354	6	2	optimizing	optimizing	NOUN
bionys-354	6	3	reactions	reaction	NOUN
bionys-354	6	4	were	be	AUX
bionys-354	6	5	conducted	conduct	VERB
bionys-354	6	6	and	and	CCONJ
bionys-354	6	7	subjected	subject	VERB
bionys-354	6	8	to	to	ADP
bionys-354	6	9	imagej	imagej	ADJ
bionys-354	6	10	software	software	NOUN
bionys-354	6	11	analysis	analysis	NOUN
bionys-354	6	12	.	.	PUNCT
bionys-354	7	1	as	as	ADP
bionys-354	7	2	a	a	DET
bionys-354	7	3	result	result	NOUN
bionys-354	7	4	,	,	PUNCT
bionys-354	7	5	4u	4u	NOUN
bionys-354	7	6	of	of	ADP
bionys-354	7	7	hotstartaq	hotstartaq	PROPN
bionys-354	7	8	and	and	CCONJ
bionys-354	7	9	125	125	NUM
bionys-354	7	10	ng	ng	PROPN
bionys-354	7	11	of	of	ADP
bionys-354	7	12	template	template	NOUN
bionys-354	7	13	dna	dna	NOUN
bionys-354	7	14	were	be	AUX
bionys-354	7	15	specified	specify	VERB
bionys-354	7	16	as	as	ADP
bionys-354	7	17	necessary	necessary	ADJ
bionys-354	7	18	for	for	ADP
bionys-354	7	19	successful	successful	ADJ
bionys-354	7	20	msp	msp	PROPN
bionys-354	7	21	reactions	reaction	NOUN
bionys-354	7	22	.	.	PUNCT
bionys-354	8	1	the	the	DET
bionys-354	8	2	confirmation	confirmation	NOUN
bionys-354	8	3	of	of	ADP
bionys-354	8	4	optimization	optimization	NOUN
bionys-354	8	5	success	success	NOUN
bionys-354	8	6	was	be	AUX
bionys-354	8	7	obtained	obtain	VERB
bionys-354	8	8	through	through	ADP
bionys-354	8	9	comparison	comparison	NOUN
bionys-354	8	10	of	of	ADP
bionys-354	8	11	semi	semi	ADJ
bionys-354	8	12	-	-	ADJ
bionys-354	8	13	quantitative	quantitative	ADJ
bionys-354	8	14	values	value	NOUN
bionys-354	8	15	of	of	ADP
bionys-354	8	16	dna	dna	PROPN
bionys-354	8	17	methylation	methylation	NOUN
bionys-354	8	18	levels	level	NOUN
bionys-354	8	19	between	between	ADP
bionys-354	8	20	reference	reference	NOUN
bionys-354	8	21	fresh	fresh	ADJ
bionys-354	8	22	frozen	frozen	ADJ
bionys-354	8	23	tissue	tissue	NOUN
bionys-354	8	24	and	and	CCONJ
bionys-354	8	25	corresponding	correspond	VERB
bionys-354	8	26	ffpe	ffpe	NOUN
bionys-354	8	27	sample	sample	NOUN
bionys-354	8	28	obtained	obtain	VERB
bionys-354	8	29	from	from	ADP
bionys-354	8	30	the	the	DET
bionys-354	8	31	same	same	ADJ
bionys-354	8	32	gbm	gbm	NOUN
bionys-354	8	33	patient	patient	NOUN
bionys-354	8	34	.	.	PUNCT
bionys-354	9	1	key	key	ADJ
bionys-354	9	2	words	word	NOUN
bionys-354	9	3	:	:	PUNCT
bionys-354	9	4	glioblastoma	glioblastoma	NOUN
bionys-354	9	5	,	,	PUNCT
bionys-354	9	6	ffpe	ffpe	NOUN
bionys-354	9	7	,	,	PUNCT
bionys-354	9	8	methylation	methylation	NOUN
bionys-354	9	9	,	,	PUNCT
bionys-354	9	10	mgmt	mgmt	PROPN
bionys-354	9	11	,	,	PUNCT
bionys-354	9	12	msp	msp	PROPN
bionys-354	9	13	,	,	PUNCT
bionys-354	9	14	optimization	optimization	NOUN
bionys-354	9	15	,	,	PUNCT
bionys-354	9	16	taq	taq	NOUN
bionys-354	9	17	polymerase	polymerase	NOUN
bionys-354	9	18	apstract	apstract	NOUN
bionys-354	9	19	:	:	PUNCT
bionys-354	9	20	optimizacija	optimizacija	VERB
bionys-354	9	21	uslova	uslova	VERB
bionys-354	9	22	msp	msp	PROPN
bionys-354	9	23	reakcije	reakcije	PROPN
bionys-354	9	24	za	za	PROPN
bionys-354	9	25	promotorni	promotorni	PROPN
bionys-354	9	26	region	region	PROPN
bionys-354	9	27	mgmt	mgmt	PROPN
bionys-354	9	28	gena	gena	PROPN
bionys-354	9	29	kod	kod	PROPN
bionys-354	9	30	ffpe	ffpe	PROPN
bionys-354	9	31	uzoraka	uzoraka	PROPN
bionys-354	9	32	glioblastoma	glioblastoma	PROPN
bionys-354	9	33	status	status	NOUN
bionys-354	9	34	metilacije	metilacije	PROPN
bionys-354	9	35	promotornog	promotornog	PROPN
bionys-354	9	36	regiona	regiona	PROPN
bionys-354	9	37	mgmt	mgmt	PROPN
bionys-354	9	38	gena	gena	PROPN
bionys-354	9	39	predstavlja	predstavlja	PROPN
bionys-354	9	40	jedan	jedan	PROPN
bionys-354	9	41	od	od	PROPN
bionys-354	9	42	najvažnijih	najvažnijih	PROPN
bionys-354	9	43	prognostičkih	prognostičkih	PROPN
bionys-354	10	1	i	i	PRON
bionys-354	10	2	prediktivnih	prediktivnih	PROPN
bionys-354	10	3	markera	markera	NOUN
bionys-354	10	4	pacijenata	pacijenata	VERB
bionys-354	10	5	obolelih	obolelih	VERB
bionys-354	10	6	od	od	NUM
bionys-354	10	7	glioblastoma	glioblastoma	NOUN
bionys-354	10	8	(	(	PUNCT
bionys-354	10	9	gbm	gbm	PROPN
bionys-354	10	10	)	)	PUNCT
bionys-354	10	11	podvrgnutih	podvrgnutih	PROPN
bionys-354	10	12	lečenju	lečenju	PROPN
bionys-354	10	13	alikirajućim	alikirajućim	PROPN
bionys-354	10	14	agensima	agensima	PROPN
bionys-354	10	15	poput	poput	PROPN
bionys-354	10	16	temozolomida	temozolomida	PROPN
bionys-354	10	17	(	(	PUNCT
bionys-354	10	18	tmz	tmz	PROPN
bionys-354	10	19	)	)	PUNCT
bionys-354	10	20	.	.	PUNCT
bionys-354	11	1	iako	iako	PROPN
bionys-354	11	2	uzorci	uzorci	PROPN
bionys-354	11	3	tkiva	tkiva	PROPN
bionys-354	11	4	fiksiranih	fiksiranih	PROPN
bionys-354	11	5	u	u	PROPN
bionys-354	11	6	formalinu	formalinu	NOUN
bionys-354	11	7	i	i	PRON
bionys-354	11	8	ukalupljenih	ukalupljenih	VERB
bionys-354	11	9	u	u	PROPN
bionys-354	11	10	parafinske	parafinske	PROPN
bionys-354	11	11	blokove	blokove	NOUN
bionys-354	11	12	(	(	PUNCT
bionys-354	11	13	ffpe	ffpe	NOUN
bionys-354	11	14	)	)	PUNCT
bionys-354	11	15	predstavljaju	predstavljaju	ADJ
bionys-354	11	16	najuobičajeniji	najuobičajeniji	PROPN
bionys-354	11	17	izvor	izvor	PROPN
bionys-354	11	18	dnk	dnk	PROPN
bionys-354	11	19	materijala	materijala	PROPN
bionys-354	11	20	prilikom	prilikom	NOUN
bionys-354	11	21	molekularno	molekularno	ADJ
bionys-354	11	22	-	-	PUNCT
bionys-354	11	23	bioloških	bioloških	NOUN
bionys-354	11	24	analiza	analiza	NOUN
bionys-354	11	25	,	,	PUNCT
bionys-354	11	26	njihova	njihova	VERB
bionys-354	11	27	upotreba	upotreba	PROPN
bionys-354	11	28	pri	pri	PROPN
bionys-354	11	29	metodi	metodi	PROPN
bionys-354	11	30	lančane	lančane	PROPN
bionys-354	11	31	reakcije	reakcije	PROPN
bionys-354	11	32	polimeraze	polimeraze	PROPN
bionys-354	11	33	specifične	specifične	PROPN
bionys-354	11	34	za	za	PROPN
bionys-354	11	35	metilaciju	metilaciju	PROPN
bionys-354	11	36	(	(	PUNCT
bionys-354	11	37	msp	msp	PROPN
bionys-354	11	38	)	)	PUNCT
bionys-354	11	39	ispoljava	ispoljava	NOUN
bionys-354	11	40	određena	određena	PROPN
bionys-354	11	41	ograničenja	ograničenja	PROPN
bionys-354	11	42	usled	usle	VERB
bionys-354	11	43	niskog	niskog	ADJ
bionys-354	11	44	integriteta	integriteta	ADJ
bionys-354	11	45	dnk	dnk	NOUN
bionys-354	11	46	.	.	PUNCT
bionys-354	12	1	cilj	cilj	ADJ
bionys-354	12	2	ovog	ovog	PROPN
bionys-354	12	3	rada	rada	PROPN
bionys-354	12	4	predstavljalo	predstavljalo	PROPN
bionys-354	12	5	je	je	PROPN
bionys-354	12	6	definisanje	definisanje	PROPN
bionys-354	12	7	optimalnih	optimalnih	PROPN
bionys-354	12	8	uslova	uslova	PROPN
bionys-354	12	9	msp	msp	PROPN
bionys-354	12	10	reakcije	reakcije	PROPN
bionys-354	12	11	za	za	PROPN
bionys-354	12	12	mgmt	mgmt	PROPN
bionys-354	12	13	promotorni	promotorni	PROPN
bionys-354	12	14	region	region	PROPN
bionys-354	12	15	prilikom	prilikom	PROPN
bionys-354	12	16	korišćenja	korišćenja	PROPN
bionys-354	12	17	bisulfitno	bisulfitno	PROPN
bionys-354	12	18	-	-	PUNCT
bionys-354	12	19	konvertovane	konvertovane	PROPN
bionys-354	12	20	dnk	dnk	NOUN
bionys-354	12	21	izolovane	izolovane	NOUN
bionys-354	12	22	iz	iz	INTJ
bionys-354	12	23	ffpe	ffpe	PROPN
bionys-354	12	24	uzoraka	uzoraka	PROPN
bionys-354	12	25	kao	kao	PROPN
bionys-354	12	26	matrice	matrice	PROPN
bionys-354	12	27	za	za	PROPN
bionys-354	12	28	msp	msp	PROPN
bionys-354	12	29	reakciju	reakciju	NOUN
bionys-354	12	30	.	.	PUNCT
bionys-354	13	1	izvedeno	izvedeno	PROPN
bionys-354	13	2	je	je	PROPN
bionys-354	13	3	nekoliko	nekoliko	NOUN
bionys-354	13	4	reakcija	reakcija	VERB
bionys-354	13	5	optimizacije	optimizacije	NOUN
bionys-354	13	6	,	,	PUNCT
bionys-354	13	7	a	a	DET
bionys-354	13	8	dobijeni	dobijeni	NOUN
bionys-354	13	9	rezultati	rezultati	NOUN
bionys-354	13	10	su	su	PROPN
bionys-354	13	11	obrađeni	obrađeni	PROPN
bionys-354	13	12	u	u	PROPN
bionys-354	13	13	imagej	imagej	ADV
bionys-354	13	14	programu	programu	NOUN
bionys-354	13	15	.	.	PUNCT
bionys-354	14	1	kao	kao	PROPN
bionys-354	14	2	rezultat	rezultat	PROPN
bionys-354	14	3	,	,	PUNCT
bionys-354	14	4	hotstartaq	hotstartaq	PROPN
bionys-354	14	5	polimeraza	polimeraza	NOUN
bionys-354	14	6	u	u	PROPN
bionys-354	14	7	količini	količini	PROPN
bionys-354	14	8	od	od	PROPN
bionys-354	14	9	4u	4u	NOUN
bionys-354	15	1	i	i	PRON
bionys-354	15	2	dnk	dnk	VERB
bionys-354	15	3	matrica	matrica	PROPN
bionys-354	15	4	u	u	PROPN
bionys-354	15	5	količini	količini	PROPN
bionys-354	15	6	od	od	PROPN
bionys-354	15	7	125	125	NUM
bionys-354	15	8	ng	ng	PROPN
bionys-354	15	9	izdvojene	izdvojene	PROPN
bionys-354	15	10	su	su	PROPN
bionys-354	15	11	kao	kao	PROPN
bionys-354	15	12	neophodne	neophodne	PROPN
bionys-354	15	13	za	za	PROPN
bionys-354	15	14	uspešnu	uspešnu	PROPN
bionys-354	15	15	msp	msp	PROPN
bionys-354	15	16	reakciju	reakciju	NOUN
bionys-354	15	17	.	.	PUNCT
bionys-354	16	1	potvrda	potvrda	ADJ
bionys-354	16	2	uspešnosti	uspešnosti	PROPN
bionys-354	16	3	reakcija	reakcija	VERB
bionys-354	16	4	optimizacije	optimizacije	PROPN
bionys-354	16	5	dobijena	dobijena	PROPN
bionys-354	16	6	je	je	PROPN
bionys-354	16	7	upoređivanjem	upoređivanjem	PROPN
bionys-354	16	8	semi	semi	ADJ
bionys-354	16	9	-	-	ADJ
bionys-354	16	10	kvantitativnih	kvantitativnih	ADJ
bionys-354	16	11	vrednosti	vrednosti	NOUN
bionys-354	16	12	nivoa	nivoa	PROPN
bionys-354	16	13	metilacije	metilacije	PROPN
bionys-354	16	14	dnk	dnk	VERB
bionys-354	16	15	između	između	NOUN
bionys-354	16	16	referentnog	referentnog	NOUN
bionys-354	16	17	sveže	sveže	PROPN
bionys-354	16	18	-	-	PUNCT
bionys-354	16	19	zamrznutog	zamrznutog	NUM
bionys-354	16	20	uzorka	uzorka	NOUN
bionys-354	16	21	(	(	PUNCT
bionys-354	16	22	ff	ff	NOUN
bionys-354	16	23	)	)	PUNCT
bionys-354	17	1	i	i	PRON
bionys-354	17	2	odgovarajućeg	odgovarajućeg	VERB
bionys-354	17	3	ffpe	ffpe	PROPN
bionys-354	17	4	uzorka	uzorka	PROPN
bionys-354	17	5	poreklom	poreklom	PROPN
bionys-354	17	6	od	od	PROPN
bionys-354	17	7	istog	istog	PROPN
bionys-354	17	8	gbm	gbm	PROPN
bionys-354	17	9	pacijenta	pacijenta	PROPN
bionys-354	17	10	.	.	PUNCT
bionys-354	18	1	ključne	ključne	PROPN
bionys-354	18	2	reči	reči	NOUN
bionys-354	18	3	:	:	PUNCT
bionys-354	18	4	glioblastomi	glioblastomi	ADJ
bionys-354	18	5	,	,	PUNCT
bionys-354	18	6	ffpe	ffpe	NOUN
bionys-354	18	7	,	,	PUNCT
bionys-354	18	8	metilacija	metilacija	NOUN
bionys-354	18	9	,	,	PUNCT
bionys-354	18	10	mgmt	mgmt	PROPN
bionys-354	18	11	,	,	PUNCT
bionys-354	18	12	msp	msp	PROPN
bionys-354	18	13	,	,	PUNCT
bionys-354	18	14	optimizacija	optimizacija	ADV
bionys-354	18	15	,	,	PUNCT
bionys-354	18	16	taq	taq	PROPN
bionys-354	18	17	polimeraza	polimeraza	VERB
bionys-354	18	18	©	©	PROPN
bionys-354	18	19	2020	2020	NUM
bionys-354	18	20	jovanovic	jovanovic	PROPN
bionys-354	18	21	et	et	PROPN
bionys-354	18	22	al	al	PROPN
bionys-354	18	23	.	.	PUNCT
bionys-354	19	1	this	this	PRON
bionys-354	19	2	is	be	AUX
bionys-354	19	3	an	an	DET
bionys-354	19	4	open	open	ADJ
bionys-354	19	5	-	-	PUNCT
bionys-354	19	6	access	access	NOUN
bionys-354	19	7	article	article	NOUN
bionys-354	19	8	distributed	distribute	VERB
bionys-354	19	9	under	under	ADP
bionys-354	19	10	the	the	DET
bionys-354	19	11	terms	term	NOUN
bionys-354	19	12	of	of	ADP
bionys-354	19	13	the	the	DET
bionys-354	19	14	creative	creative	ADJ
bionys-354	19	15	commons	common	NOUN
bionys-354	19	16	attribution	attribution	NOUN
bionys-354	19	17	license	license	NOUN
bionys-354	19	18	,	,	PUNCT
bionys-354	19	19	which	which	PRON
bionys-354	19	20	permits	permit	VERB
bionys-354	19	21	unrestricted	unrestricted	ADJ
bionys-354	19	22	use	use	NOUN
bionys-354	19	23	,	,	PUNCT
bionys-354	19	24	distribution	distribution	NOUN
bionys-354	19	25	,	,	PUNCT
bionys-354	19	26	and	and	CCONJ
bionys-354	19	27	build	build	VERB
bionys-354	19	28	upon	upon	SCONJ
bionys-354	19	29	your	your	PRON
bionys-354	19	30	work	work	NOUN
bionys-354	19	31	non	non	ADJ
bionys-354	19	32	-	-	ADJ
bionys-354	19	33	commercially	commercially	ADV
bionys-354	19	34	under	under	ADP
bionys-354	19	35	the	the	DET
bionys-354	19	36	same	same	ADJ
bionys-354	19	37	license	license	NOUN
bionys-354	19	38	as	as	ADP
bionys-354	19	39	the	the	DET
bionys-354	19	40	original	original	ADJ
bionys-354	19	41	.	.	PUNCT
bionys-354	20	1	139	139	NUM
bionys-354	20	2	introduction	introduction	NOUN
bionys-354	20	3	glioblastoma	glioblastoma	NOUN
bionys-354	20	4	(	(	PUNCT
bionys-354	20	5	gbm	gbm	PROPN
bionys-354	20	6	)	)	PUNCT
bionys-354	20	7	refers	refer	VERB
bionys-354	20	8	to	to	ADP
bionys-354	20	9	the	the	DET
bionys-354	20	10	highly	highly	ADV
bionys-354	20	11	infiltrative	infiltrative	ADJ
bionys-354	20	12	type	type	NOUN
bionys-354	20	13	of	of	ADP
bionys-354	20	14	gliomas	glioma	NOUN
bionys-354	20	15	,	,	PUNCT
bionys-354	20	16	malignant	malignant	ADJ
bionys-354	20	17	brain	brain	NOUN
bionys-354	20	18	tumors	tumor	NOUN
bionys-354	20	19	.	.	PUNCT
bionys-354	21	1	owing	owe	VERB
bionys-354	21	2	to	to	ADP
bionys-354	21	3	great	great	ADJ
bionys-354	21	4	morphological	morphological	ADJ
bionys-354	21	5	and	and	CCONJ
bionys-354	21	6	genetic	genetic	ADJ
bionys-354	21	7	heterogeneity	heterogeneity	NOUN
bionys-354	21	8	,	,	PUNCT
bionys-354	21	9	it	it	PRON
bionys-354	21	10	is	be	AUX
bionys-354	21	11	characterized	characterize	VERB
bionys-354	21	12	by	by	ADP
bionys-354	21	13	extremely	extremely	ADV
bionys-354	21	14	low	low	ADJ
bionys-354	21	15	five	five	NUM
bionys-354	21	16	-	-	PUNCT
bionys-354	21	17	year	year	NOUN
bionys-354	21	18	survival	survival	NOUN
bionys-354	21	19	rates	rate	NOUN
bionys-354	21	20	–	–	PUNCT
bionys-354	21	21	only	only	ADV
bionys-354	21	22	5	5	NUM
bionys-354	21	23	%	%	NOUN
bionys-354	21	24	,	,	PUNCT
bionys-354	21	25	and	and	CCONJ
bionys-354	21	26	an	an	DET
bionys-354	21	27	annual	annual	ADJ
bionys-354	21	28	incidence	incidence	NOUN
bionys-354	21	29	of	of	ADP
bionys-354	21	30	5.26	5.26	NUM
bionys-354	21	31	per	per	ADP
bionys-354	21	32	100,000	100,000	NUM
bionys-354	21	33	people	people	NOUN
bionys-354	21	34	(	(	PUNCT
bionys-354	21	35	soomro	soomro	PROPN
bionys-354	21	36	et	et	PROPN
bionys-354	21	37	al	al	PROPN
bionys-354	21	38	.	.	PROPN
bionys-354	21	39	,	,	PUNCT
bionys-354	21	40	2017	2017	NUM
bionys-354	21	41	)	)	PUNCT
bionys-354	21	42	.	.	PUNCT
bionys-354	22	1	although	although	SCONJ
bionys-354	22	2	it	it	PRON
bionys-354	22	3	is	be	AUX
bionys-354	22	4	widely	widely	ADV
bionys-354	22	5	accepted	accept	VERB
bionys-354	22	6	that	that	SCONJ
bionys-354	22	7	gliomas	glioma	NOUN
bionys-354	22	8	originate	originate	VERB
bionys-354	22	9	from	from	ADP
bionys-354	22	10	normal	normal	ADJ
bionys-354	22	11	glial	glial	ADJ
bionys-354	22	12	cells	cell	NOUN
bionys-354	22	13	,	,	PUNCT
bionys-354	22	14	increasing	increase	VERB
bionys-354	22	15	evidence	evidence	NOUN
bionys-354	22	16	supports	support	VERB
bionys-354	22	17	the	the	DET
bionys-354	22	18	role	role	NOUN
bionys-354	22	19	of	of	ADP
bionys-354	22	20	various	various	ADJ
bionys-354	22	21	cell	cell	NOUN
bionys-354	22	22	types	type	NOUN
bionys-354	22	23	such	such	ADJ
bionys-354	22	24	as	as	ADP
bionys-354	22	25	glial	glial	ADJ
bionys-354	22	26	or	or	CCONJ
bionys-354	22	27	neural	neural	ADJ
bionys-354	22	28	precursors	precursor	NOUN
bionys-354	22	29	and	and	CCONJ
bionys-354	22	30	stem	stem	NOUN
bionys-354	22	31	cells	cell	NOUN
bionys-354	22	32	as	as	ADP
bionys-354	22	33	their	their	PRON
bionys-354	22	34	possible	possible	ADJ
bionys-354	22	35	origin	origin	NOUN
bionys-354	22	36	(	(	PUNCT
bionys-354	22	37	chen	chen	PROPN
bionys-354	22	38	et	et	PROPN
bionys-354	22	39	al	al	PROPN
bionys-354	22	40	.	.	PROPN
bionys-354	22	41	,	,	PUNCT
bionys-354	22	42	2017	2017	NUM
bionys-354	22	43	)	)	PUNCT
bionys-354	22	44	.	.	PUNCT
bionys-354	23	1	recent	recent	ADJ
bionys-354	23	2	findings	finding	NOUN
bionys-354	23	3	140	140	NUM
bionys-354	23	4	in	in	ADP
bionys-354	23	5	the	the	DET
bionys-354	23	6	molecular	molecular	ADJ
bionys-354	23	7	biology	biology	NOUN
bionys-354	23	8	of	of	ADP
bionys-354	23	9	glioma	glioma	NOUN
bionys-354	23	10	have	have	AUX
bionys-354	23	11	led	lead	VERB
bionys-354	23	12	to	to	ADP
bionys-354	23	13	the	the	DET
bionys-354	23	14	novel	novel	NOUN
bionys-354	23	15	,	,	PUNCT
bionys-354	23	16	improved	improved	ADJ
bionys-354	23	17	system	system	NOUN
bionys-354	23	18	of	of	ADP
bionys-354	23	19	classification	classification	NOUN
bionys-354	23	20	which	which	PRON
bionys-354	23	21	was	be	AUX
bionys-354	23	22	established	establish	VERB
bionys-354	23	23	by	by	ADP
bionys-354	23	24	the	the	DET
bionys-354	23	25	world	world	PROPN
bionys-354	23	26	health	health	PROPN
bionys-354	23	27	organization	organization	NOUN
bionys-354	23	28	(	(	PUNCT
bionys-354	23	29	who	who	PRON
bionys-354	23	30	)	)	PUNCT
bionys-354	23	31	in	in	ADP
bionys-354	23	32	2016	2016	NUM
bionys-354	23	33	(	(	PUNCT
bionys-354	23	34	louis	louis	PROPN
bionys-354	23	35	et	et	PROPN
bionys-354	23	36	al	al	PROPN
bionys-354	23	37	.	.	PROPN
bionys-354	23	38	,	,	PUNCT
bionys-354	23	39	2016	2016	NUM
bionys-354	23	40	)	)	PUNCT
bionys-354	23	41	.	.	PUNCT
bionys-354	24	1	namely	namely	ADV
bionys-354	24	2	,	,	PUNCT
bionys-354	24	3	the	the	DET
bionys-354	24	4	shortcomings	shortcoming	NOUN
bionys-354	24	5	of	of	ADP
bionys-354	24	6	histopathology	histopathology	NOUN
bionys-354	24	7	based	base	VERB
bionys-354	24	8	classification	classification	NOUN
bionys-354	24	9	were	be	AUX
bionys-354	24	10	exceeded	exceed	VERB
bionys-354	24	11	through	through	ADP
bionys-354	24	12	the	the	DET
bionys-354	24	13	incorporation	incorporation	NOUN
bionys-354	24	14	of	of	ADP
bionys-354	24	15	molecular	molecular	ADJ
bionys-354	24	16	diagnostic	diagnostic	ADJ
bionys-354	24	17	criteria	criterion	NOUN
bionys-354	24	18	testing	test	VERB
bionys-354	24	19	for	for	ADP
bionys-354	24	20	isocitrate	isocitrate	ADJ
bionys-354	24	21	dehydrogenase	dehydrogenase	NOUN
bionys-354	24	22	(	(	PUNCT
bionys-354	24	23	idh	idh	NOUN
bionys-354	24	24	)	)	PUNCT
bionys-354	24	25	mutation	mutation	NOUN
bionys-354	24	26	,	,	PUNCT
bionys-354	24	27	chromosome	chromosome	NOUN
bionys-354	24	28	1p/19q	1p/19q	NOUN
bionys-354	24	29	deletion	deletion	NOUN
bionys-354	24	30	,	,	PUNCT
bionys-354	24	31	and	and	CCONJ
bionys-354	24	32	histone	histone	NOUN
bionys-354	24	33	mutations	mutation	NOUN
bionys-354	24	34	.	.	PUNCT
bionys-354	25	1	in	in	ADP
bionys-354	25	2	2010	2010	NUM
bionys-354	25	3	,	,	PUNCT
bionys-354	25	4	the	the	DET
bionys-354	25	5	cancer	cancer	NOUN
bionys-354	25	6	genome	genome	NOUN
bionys-354	25	7	atlas	atlas	PROPN
bionys-354	25	8	(	(	PUNCT
bionys-354	25	9	tcga	tcga	PROPN
bionys-354	25	10	)	)	PUNCT
bionys-354	25	11	classification	classification	NOUN
bionys-354	25	12	of	of	ADP
bionys-354	25	13	gbms	gbms	NOUN
bionys-354	25	14	had	have	AUX
bionys-354	25	15	identified	identify	VERB
bionys-354	25	16	4	4	NUM
bionys-354	25	17	subtypes	subtype	NOUN
bionys-354	25	18	of	of	ADP
bionys-354	25	19	glioblastomas	glioblastoma	NOUN
bionys-354	25	20	based	base	VERB
bionys-354	25	21	upon	upon	SCONJ
bionys-354	25	22	gene	gene	NOUN
bionys-354	25	23	expression	expression	NOUN
bionys-354	25	24	profiles	profile	NOUN
bionys-354	25	25	and	and	CCONJ
bionys-354	25	26	genomic	genomic	NOUN
bionys-354	25	27	clustering	clustering	NOUN
bionys-354	25	28	–	–	PUNCT
bionys-354	25	29	proneural	proneural	ADJ
bionys-354	25	30	,	,	PUNCT
bionys-354	25	31	neural	neural	ADJ
bionys-354	25	32	,	,	PUNCT
bionys-354	25	33	classical	classical	ADJ
bionys-354	25	34	,	,	PUNCT
bionys-354	25	35	and	and	CCONJ
bionys-354	25	36	mesenchymal	mesenchymal	ADJ
bionys-354	25	37	subtypes	subtype	NOUN
bionys-354	25	38	(	(	PUNCT
bionys-354	25	39	verhaak	verhaak	NOUN
bionys-354	25	40	et	et	PROPN
bionys-354	25	41	al	al	PROPN
bionys-354	25	42	.	.	PROPN
bionys-354	25	43	,	,	PUNCT
bionys-354	25	44	2010	2010	NUM
bionys-354	25	45	)	)	PUNCT
bionys-354	25	46	.	.	PUNCT
bionys-354	26	1	the	the	DET
bionys-354	26	2	methylation	methylation	NOUN
bionys-354	26	3	of	of	ADP
bionys-354	26	4	the	the	DET
bionys-354	26	5	cytosine	cytosine	NOUN
bionys-354	26	6	-	-	PUNCT
bionys-354	26	7	phosphate	phosphate	NOUN
bionys-354	26	8	-	-	PUNCT
bionys-354	26	9	guanine	guanine	NOUN
bionys-354	26	10	(	(	PUNCT
bionys-354	26	11	cpg	cpg	NOUN
bionys-354	26	12	)	)	PUNCT
bionys-354	26	13	islands	island	NOUN
bionys-354	26	14	in	in	ADP
bionys-354	26	15	the	the	DET
bionys-354	26	16	promoter	promoter	NOUN
bionys-354	26	17	region	region	NOUN
bionys-354	26	18	of	of	ADP
bionys-354	26	19	o6	o6	NOUN
bionys-354	26	20	-	-	PUNCT
bionys-354	26	21	methyl	methyl	NOUN
bionys-354	26	22	guanine	guanine	NOUN
bionys-354	26	23	-	-	PUNCT
bionys-354	26	24	dna	dna	PROPN
bionys-354	26	25	methyltransferase	methyltransferase	NOUN
bionys-354	26	26	gene	gene	NOUN
bionys-354	26	27	(	(	PUNCT
bionys-354	26	28	mgmt	mgmt	PROPN
bionys-354	26	29	)	)	PUNCT
bionys-354	26	30	represents	represent	VERB
bionys-354	26	31	one	one	NUM
bionys-354	26	32	of	of	ADP
bionys-354	26	33	the	the	DET
bionys-354	26	34	most	most	ADV
bionys-354	26	35	common	common	ADJ
bionys-354	26	36	epigenetic	epigenetic	ADJ
bionys-354	26	37	alterations	alteration	NOUN
bionys-354	26	38	in	in	ADP
bionys-354	26	39	gbm	gbm	NOUN
bionys-354	26	40	.	.	PUNCT
bionys-354	27	1	it	it	PRON
bionys-354	27	2	is	be	AUX
bionys-354	27	3	present	present	ADJ
bionys-354	27	4	in	in	ADP
bionys-354	27	5	both	both	CCONJ
bionys-354	27	6	primary	primary	ADJ
bionys-354	27	7	and	and	CCONJ
bionys-354	27	8	secondary	secondary	ADJ
bionys-354	27	9	gbm	gbm	NOUN
bionys-354	27	10	in	in	ADP
bionys-354	27	11	42	42	NUM
bionys-354	27	12	%	%	NOUN
bionys-354	27	13	and	and	CCONJ
bionys-354	27	14	79	79	NUM
bionys-354	27	15	%	%	NOUN
bionys-354	27	16	patients	patient	NOUN
bionys-354	27	17	respectively	respectively	ADV
bionys-354	27	18	(	(	PUNCT
bionys-354	27	19	soomro	soomro	PROPN
bionys-354	27	20	et	et	PROPN
bionys-354	27	21	al	al	PROPN
bionys-354	27	22	.	.	PROPN
bionys-354	27	23	,	,	PUNCT
bionys-354	27	24	2017	2017	NUM
bionys-354	27	25	)	)	PUNCT
bionys-354	27	26	.	.	PUNCT
bionys-354	28	1	the	the	DET
bionys-354	28	2	mgmt	mgmt	NOUN
bionys-354	28	3	enzyme	enzyme	NOUN
bionys-354	28	4	is	be	AUX
bionys-354	28	5	an	an	DET
bionys-354	28	6	excision	excision	NOUN
bionys-354	28	7	repair	repair	NOUN
bionys-354	28	8	enzyme	enzyme	NOUN
bionys-354	28	9	that	that	PRON
bionys-354	28	10	removes	remove	VERB
bionys-354	28	11	alkyl	alkyl	NOUN
bionys-354	28	12	adducts	adduct	NOUN
bionys-354	28	13	from	from	ADP
bionys-354	28	14	the	the	DET
bionys-354	28	15	o6position	o6position	NOUN
bionys-354	28	16	of	of	ADP
bionys-354	28	17	the	the	DET
bionys-354	28	18	guanine	guanine	NOUN
bionys-354	28	19	.	.	PUNCT
bionys-354	29	1	thus	thus	ADV
bionys-354	29	2	,	,	PUNCT
bionys-354	29	3	it	it	PRON
bionys-354	29	4	protects	protect	VERB
bionys-354	29	5	normal	normal	ADJ
bionys-354	29	6	cells	cell	NOUN
bionys-354	29	7	from	from	ADP
bionys-354	29	8	carcinogens	carcinogen	NOUN
bionys-354	29	9	by	by	ADP
bionys-354	29	10	repairing	repair	VERB
bionys-354	29	11	double	double	ADJ
bionys-354	29	12	-	-	PUNCT
bionys-354	29	13	strand	strand	NOUN
bionys-354	29	14	breaks	break	NOUN
bionys-354	29	15	and	and	CCONJ
bionys-354	29	16	base	base	NOUN
bionys-354	29	17	mispairing	mispairing	NOUN
bionys-354	29	18	which	which	PRON
bionys-354	29	19	leads	lead	VERB
bionys-354	29	20	to	to	ADP
bionys-354	29	21	apoptosis	apoptosis	NOUN
bionys-354	29	22	and	and	CCONJ
bionys-354	29	23	cell	cell	NOUN
bionys-354	29	24	death	death	NOUN
bionys-354	29	25	.	.	PUNCT
bionys-354	30	1	during	during	ADP
bionys-354	30	2	that	that	DET
bionys-354	30	3	process	process	NOUN
bionys-354	30	4	it	it	PRON
bionys-354	30	5	is	be	AUX
bionys-354	30	6	being	be	AUX
bionys-354	30	7	irreversibly	irreversibly	ADV
bionys-354	30	8	deactivated	deactivate	VERB
bionys-354	30	9	,	,	PUNCT
bionys-354	30	10	which	which	PRON
bionys-354	30	11	is	be	AUX
bionys-354	30	12	why	why	SCONJ
bionys-354	30	13	mgmt	mgmt	NOUN
bionys-354	30	14	is	be	AUX
bionys-354	30	15	referred	refer	VERB
bionys-354	30	16	to	to	ADP
bionys-354	30	17	as	as	ADP
bionys-354	30	18	“	"	PUNCT
bionys-354	30	19	suicidal	suicidal	ADJ
bionys-354	30	20	”	"	PUNCT
bionys-354	30	21	enzyme	enzyme	NOUN
bionys-354	30	22	(	(	PUNCT
bionys-354	30	23	thon	thon	PROPN
bionys-354	30	24	et	et	PROPN
bionys-354	30	25	al	al	PROPN
bionys-354	30	26	.	.	PROPN
bionys-354	30	27	,	,	PUNCT
bionys-354	30	28	2013	2013	NUM
bionys-354	30	29	;	;	PUNCT
bionys-354	30	30	soomro	soomro	PROPN
bionys-354	30	31	et	et	PROPN
bionys-354	30	32	al	al	PROPN
bionys-354	30	33	.	.	PROPN
bionys-354	30	34	,	,	PUNCT
bionys-354	30	35	2017	2017	NUM
bionys-354	30	36	)	)	PUNCT
bionys-354	30	37	.	.	PUNCT
bionys-354	31	1	regarding	regard	VERB
bionys-354	31	2	the	the	DET
bionys-354	31	3	mgmt	mgmt	ADJ
bionys-354	31	4	role	role	NOUN
bionys-354	31	5	of	of	ADP
bionys-354	31	6	counteracting	counteract	VERB
bionys-354	31	7	the	the	DET
bionys-354	31	8	activity	activity	NOUN
bionys-354	31	9	of	of	ADP
bionys-354	31	10	alkylating	alkylate	VERB
bionys-354	31	11	agents	agent	NOUN
bionys-354	31	12	,	,	PUNCT
bionys-354	31	13	its	its	PRON
bionys-354	31	14	inactivation	inactivation	NOUN
bionys-354	31	15	through	through	ADP
bionys-354	31	16	epigenetic	epigenetic	ADJ
bionys-354	31	17	silencing	silencing	NOUN
bionys-354	31	18	was	be	AUX
bionys-354	31	19	recognized	recognize	VERB
bionys-354	31	20	as	as	ADP
bionys-354	31	21	an	an	DET
bionys-354	31	22	important	important	ADJ
bionys-354	31	23	and	and	CCONJ
bionys-354	31	24	clinically	clinically	ADV
bionys-354	31	25	relevant	relevant	ADJ
bionys-354	31	26	factor	factor	NOUN
bionys-354	31	27	in	in	ADP
bionys-354	31	28	gbm	gbm	NOUN
bionys-354	31	29	patients	patient	NOUN
bionys-354	31	30	undergoing	undergo	VERB
bionys-354	31	31	treatment	treatment	NOUN
bionys-354	31	32	with	with	ADP
bionys-354	31	33	alkylating	alkylate	VERB
bionys-354	31	34	agents	agent	NOUN
bionys-354	31	35	such	such	ADJ
bionys-354	31	36	as	as	ADP
bionys-354	31	37	temozolomide	temozolomide	NOUN
bionys-354	31	38	(	(	PUNCT
bionys-354	31	39	tmz	tmz	PROPN
bionys-354	31	40	)	)	PUNCT
bionys-354	31	41	.	.	PUNCT
bionys-354	32	1	the	the	DET
bionys-354	32	2	positive	positive	ADJ
bionys-354	32	3	methylation	methylation	NOUN
bionys-354	32	4	status	status	NOUN
bionys-354	32	5	of	of	ADP
bionys-354	32	6	the	the	DET
bionys-354	32	7	mgmt	mgmt	NOUN
bionys-354	32	8	promoter	promoter	NOUN
bionys-354	32	9	represents	represent	VERB
bionys-354	32	10	a	a	DET
bionys-354	32	11	strong	strong	ADJ
bionys-354	32	12	and	and	CCONJ
bionys-354	32	13	independent	independent	ADJ
bionys-354	32	14	predictive	predictive	ADJ
bionys-354	32	15	factor	factor	NOUN
bionys-354	32	16	of	of	ADP
bionys-354	32	17	favorable	favorable	ADJ
bionys-354	32	18	survival	survival	NOUN
bionys-354	32	19	in	in	ADP
bionys-354	32	20	gbm	gbm	PROPN
bionys-354	32	21	.	.	PUNCT
bionys-354	33	1	the	the	DET
bionys-354	33	2	median	median	PROPN
bionys-354	33	3	survival	survival	NOUN
bionys-354	33	4	was	be	AUX
bionys-354	33	5	significantly	significantly	ADV
bionys-354	33	6	longer	long	ADV
bionys-354	33	7	in	in	ADP
bionys-354	33	8	patients	patient	NOUN
bionys-354	33	9	with	with	ADP
bionys-354	33	10	a	a	DET
bionys-354	33	11	methylated	methylate	VERB
bionys-354	33	12	mgmt	mgmt	NOUN
bionys-354	33	13	promoter	promoter	NOUN
bionys-354	33	14	(	(	PUNCT
bionys-354	33	15	21.7	21.7	NUM
bionys-354	33	16	months	month	NOUN
bionys-354	33	17	)	)	PUNCT
bionys-354	33	18	in	in	ADP
bionys-354	33	19	comparison	comparison	NOUN
bionys-354	33	20	with	with	ADP
bionys-354	33	21	the	the	DET
bionys-354	33	22	patients	patient	NOUN
bionys-354	33	23	lacking	lack	VERB
bionys-354	33	24	methylation	methylation	NOUN
bionys-354	33	25	(	(	PUNCT
bionys-354	33	26	12.7	12.7	NUM
bionys-354	33	27	months	month	NOUN
bionys-354	33	28	)	)	PUNCT
bionys-354	33	29	.	.	PUNCT
bionys-354	34	1	additionally	additionally	ADV
bionys-354	34	2	,	,	PUNCT
bionys-354	34	3	a	a	DET
bionys-354	34	4	high	high	ADJ
bionys-354	34	5	frequency	frequency	NOUN
bionys-354	34	6	of	of	ADP
bionys-354	34	7	mgmt	mgmt	PROPN
bionys-354	34	8	promoter	promoter	NOUN
bionys-354	34	9	methylation	methylation	NOUN
bionys-354	34	10	was	be	AUX
bionys-354	34	11	documented	document	VERB
bionys-354	34	12	in	in	ADP
bionys-354	34	13	long	long	ADJ
bionys-354	34	14	-	-	PUNCT
bionys-354	34	15	term	term	NOUN
bionys-354	34	16	gbm	gbm	NOUN
bionys-354	34	17	survivors	survivor	NOUN
bionys-354	34	18	undergoing	undergo	VERB
bionys-354	34	19	tmz	tmz	NOUN
bionys-354	34	20	treatment	treatment	NOUN
bionys-354	34	21	(	(	PUNCT
bionys-354	34	22	thon	thon	PROPN
bionys-354	34	23	et	et	PROPN
bionys-354	34	24	al	al	PROPN
bionys-354	34	25	.	.	PROPN
bionys-354	34	26	,	,	PUNCT
bionys-354	34	27	2011	2011	NUM
bionys-354	34	28	;	;	PUNCT
bionys-354	34	29	thon	thon	PROPN
bionys-354	34	30	et	et	PROPN
bionys-354	34	31	al	al	PROPN
bionys-354	34	32	.	.	PROPN
bionys-354	34	33	,	,	PUNCT
bionys-354	34	34	2013	2013	NUM
bionys-354	34	35	)	)	PUNCT
bionys-354	34	36	.	.	PUNCT
bionys-354	35	1	given	give	VERB
bionys-354	35	2	the	the	DET
bionys-354	35	3	fact	fact	NOUN
bionys-354	35	4	that	that	SCONJ
bionys-354	35	5	methylated	methylate	VERB
bionys-354	35	6	cytosine	cytosine	NOUN
bionys-354	35	7	in	in	ADP
bionys-354	35	8	cpg	cpg	PROPN
bionys-354	35	9	islands	island	NOUN
bionys-354	35	10	exhibits	exhibit	VERB
bionys-354	35	11	the	the	DET
bionys-354	35	12	same	same	ADJ
bionys-354	35	13	base	base	NOUN
bionys-354	35	14	-	-	PUNCT
bionys-354	35	15	pairing	pair	VERB
bionys-354	35	16	interactions	interaction	NOUN
bionys-354	35	17	as	as	ADP
bionys-354	35	18	unmethylated	unmethylated	ADJ
bionys-354	35	19	cytosine	cytosine	NOUN
bionys-354	35	20	,	,	PUNCT
bionys-354	35	21	the	the	DET
bionys-354	35	22	evaluation	evaluation	NOUN
bionys-354	35	23	of	of	ADP
bionys-354	35	24	methylation	methylation	NOUN
bionys-354	35	25	status	status	NOUN
bionys-354	35	26	using	use	VERB
bionys-354	35	27	conventional	conventional	ADJ
bionys-354	35	28	hybridization	hybridization	NOUN
bionys-354	35	29	-	-	PUNCT
bionys-354	35	30	based	base	VERB
bionys-354	35	31	methods	method	NOUN
bionys-354	35	32	,	,	PUNCT
bionys-354	35	33	i.e.	i.e.	X
bionys-354	35	34	microarrays	microarray	NOUN
bionys-354	35	35	and	and	CCONJ
bionys-354	35	36	pcr	pcr	NOUN
bionys-354	35	37	,	,	PUNCT
bionys-354	35	38	is	be	AUX
bionys-354	35	39	not	not	PART
bionys-354	35	40	suitable	suitable	ADJ
bionys-354	35	41	(	(	PUNCT
bionys-354	35	42	holmes	holmes	PROPN
bionys-354	35	43	et	et	PROPN
bionys-354	35	44	al	al	PROPN
bionys-354	35	45	.	.	PROPN
bionys-354	35	46	,	,	PUNCT
bionys-354	35	47	2014	2014	NUM
bionys-354	35	48	)	)	PUNCT
bionys-354	35	49	.	.	PUNCT
bionys-354	36	1	to	to	PART
bionys-354	36	2	address	address	VERB
bionys-354	36	3	that	that	SCONJ
bionys-354	36	4	,	,	PUNCT
bionys-354	36	5	frommer	frommer	PROPN
bionys-354	36	6	et	et	PROPN
bionys-354	36	7	al	al	PROPN
bionys-354	36	8	.	.	PROPN
bionys-354	36	9	have	have	AUX
bionys-354	36	10	designed	design	VERB
bionys-354	36	11	a	a	DET
bionys-354	36	12	protocol	protocol	NOUN
bionys-354	36	13	that	that	PRON
bionys-354	36	14	utilizes	utilize	VERB
bionys-354	36	15	bisulfite	bisulfite	NOUN
bionys-354	36	16	–	–	PUNCT
bionys-354	36	17	induced	induce	VERB
bionys-354	36	18	modification	modification	NOUN
bionys-354	36	19	of	of	ADP
bionys-354	36	20	genomic	genomic	ADJ
bionys-354	36	21	dna	dna	NOUN
bionys-354	36	22	resulting	result	VERB
bionys-354	36	23	in	in	ADP
bionys-354	36	24	the	the	DET
bionys-354	36	25	conversion	conversion	NOUN
bionys-354	36	26	of	of	ADP
bionys-354	36	27	unmethylated	unmethylated	ADJ
bionys-354	36	28	cytosine	cytosine	NOUN
bionys-354	36	29	to	to	ADP
bionys-354	36	30	uracil	uracil	NUM
bionys-354	36	31	,	,	PUNCT
bionys-354	36	32	while	while	SCONJ
bionys-354	36	33	5	5	NUM
bionys-354	36	34	-	-	PUNCT
bionys-354	36	35	methylcytosines	methylcytosine	NOUN
bionys-354	36	36	remain	remain	VERB
bionys-354	36	37	intact	intact	ADJ
bionys-354	36	38	(	(	PUNCT
bionys-354	36	39	frommer	frommer	NOUN
bionys-354	36	40	et	et	PROPN
bionys-354	36	41	al	al	PROPN
bionys-354	36	42	.	.	PROPN
bionys-354	36	43	,	,	PUNCT
bionys-354	36	44	1992	1992	NUM
bionys-354	36	45	)	)	PUNCT
bionys-354	36	46	.	.	PUNCT
bionys-354	37	1	such	such	ADJ
bionys-354	37	2	bisulfite	bisulfite	NOUN
bionys-354	37	3	-	-	PUNCT
bionys-354	37	4	converted	convert	VERB
bionys-354	37	5	dna	dna	NOUN
bionys-354	37	6	could	could	AUX
bionys-354	37	7	be	be	AUX
bionys-354	37	8	subsequently	subsequently	ADV
bionys-354	37	9	analyzed	analyze	VERB
bionys-354	37	10	via	via	ADP
bionys-354	37	11	pcr	pcr	NOUN
bionys-354	37	12	in	in	ADP
bionys-354	37	13	order	order	NOUN
bionys-354	37	14	to	to	PART
bionys-354	37	15	acquire	acquire	VERB
bionys-354	37	16	desired	desire	VERB
bionys-354	37	17	epigenetic	epigenetic	ADJ
bionys-354	37	18	information	information	NOUN
bionys-354	37	19	.	.	PUNCT
bionys-354	38	1	with	with	ADP
bionys-354	38	2	the	the	DET
bionys-354	38	3	numerous	numerous	ADJ
bionys-354	38	4	commercially	commercially	ADV
bionys-354	38	5	available	available	ADJ
bionys-354	38	6	kits	kit	NOUN
bionys-354	38	7	and	and	CCONJ
bionys-354	38	8	several	several	ADJ
bionys-354	38	9	technological	technological	ADJ
bionys-354	38	10	advances	advance	NOUN
bionys-354	38	11	,	,	PUNCT
bionys-354	38	12	current	current	ADJ
bionys-354	38	13	bisulfite	bisulfite	NOUN
bionys-354	38	14	-	-	PUNCT
bionys-354	38	15	treatment	treatment	NOUN
bionys-354	38	16	protocols	protocol	NOUN
bionys-354	38	17	are	be	AUX
bionys-354	38	18	more	more	ADV
bionys-354	38	19	convenient	convenient	ADJ
bionys-354	38	20	and	and	CCONJ
bionys-354	38	21	user	user	NOUN
bionys-354	38	22	friendly	friendly	ADJ
bionys-354	38	23	in	in	ADP
bionys-354	38	24	comparison	comparison	NOUN
bionys-354	38	25	with	with	ADP
bionys-354	38	26	the	the	DET
bionys-354	38	27	original	original	ADJ
bionys-354	38	28	16	16	NUM
bionys-354	38	29	hours	hour	NOUN
bionys-354	38	30	protocol	protocol	NOUN
bionys-354	38	31	(	(	PUNCT
bionys-354	38	32	holmes	holmes	PROPN
bionys-354	38	33	et	et	PROPN
bionys-354	38	34	al	al	PROPN
bionys-354	38	35	.	.	PROPN
bionys-354	38	36	,	,	PUNCT
bionys-354	38	37	2014	2014	NUM
bionys-354	38	38	)	)	PUNCT
bionys-354	38	39	.	.	PUNCT
bionys-354	39	1	however	however	ADV
bionys-354	39	2	,	,	PUNCT
bionys-354	39	3	the	the	DET
bionys-354	39	4	success	success	NOUN
bionys-354	39	5	of	of	ADP
bionys-354	39	6	bisulfite	bisulfite	NOUN
bionys-354	39	7	-	-	PUNCT
bionys-354	39	8	conversion	conversion	NOUN
bionys-354	39	9	may	may	AUX
bionys-354	39	10	vary	vary	VERB
bionys-354	39	11	considerably	considerably	ADV
bionys-354	39	12	depending	depend	VERB
bionys-354	39	13	on	on	ADP
bionys-354	39	14	the	the	DET
bionys-354	39	15	quality	quality	NOUN
bionys-354	39	16	of	of	ADP
bionys-354	39	17	dna	dna	PROPN
bionys-354	39	18	samples	sample	NOUN
bionys-354	39	19	and	and	CCONJ
bionys-354	39	20	the	the	DET
bionys-354	39	21	choice	choice	NOUN
bionys-354	39	22	of	of	ADP
bionys-354	39	23	tissue	tissue	NOUN
bionys-354	39	24	samples	sample	NOUN
bionys-354	39	25	(	(	PUNCT
bionys-354	39	26	tournier	tournier	X
bionys-354	39	27	et	et	PROPN
bionys-354	39	28	al	al	PROPN
bionys-354	39	29	.	.	PROPN
bionys-354	39	30	,	,	PUNCT
bionys-354	39	31	2012	2012	NUM
bionys-354	39	32	)	)	PUNCT
bionys-354	39	33	.	.	PUNCT
bionys-354	40	1	there	there	PRON
bionys-354	40	2	are	be	VERB
bionys-354	40	3	strong	strong	ADJ
bionys-354	40	4	suggestions	suggestion	NOUN
bionys-354	40	5	for	for	ADP
bionys-354	40	6	avoiding	avoid	VERB
bionys-354	40	7	formalin	formalin	NOUN
bionys-354	40	8	-	-	PUNCT
bionys-354	40	9	fixed	fix	VERB
bionys-354	40	10	and	and	CCONJ
bionys-354	40	11	paraffin	paraffin	NOUN
bionys-354	40	12	-	-	PUNCT
bionys-354	40	13	embedded	embed	VERB
bionys-354	40	14	tissues	tissue	NOUN
bionys-354	40	15	(	(	PUNCT
bionys-354	40	16	ffpe	ffpe	NOUN
bionys-354	40	17	)	)	PUNCT
bionys-354	40	18	samples	sample	NOUN
bionys-354	40	19	for	for	ADP
bionys-354	40	20	bisulfite	bisulfite	NOUN
bionys-354	40	21	conversion	conversion	NOUN
bionys-354	40	22	and	and	CCONJ
bionys-354	40	23	following	follow	VERB
bionys-354	40	24	methylation	methylation	NOUN
bionys-354	40	25	-	-	PUNCT
bionys-354	40	26	specific	specific	ADJ
bionys-354	40	27	polymerase	polymerase	NOUN
bionys-354	40	28	chain	chain	NOUN
bionys-354	40	29	reaction	reaction	NOUN
bionys-354	40	30	(	(	PUNCT
bionys-354	40	31	msp	msp	PROPN
bionys-354	40	32	)	)	PUNCT
bionys-354	40	33	.	.	PUNCT
bionys-354	41	1	these	these	PRON
bionys-354	41	2	are	be	AUX
bionys-354	41	3	supported	support	VERB
bionys-354	41	4	by	by	ADP
bionys-354	41	5	evidence	evidence	NOUN
bionys-354	41	6	that	that	SCONJ
bionys-354	41	7	the	the	DET
bionys-354	41	8	use	use	NOUN
bionys-354	41	9	of	of	ADP
bionys-354	41	10	ffpe	ffpe	NOUN
bionys-354	41	11	induces	induce	VERB
bionys-354	41	12	non	non	ADJ
bionys-354	41	13	-	-	ADJ
bionys-354	41	14	reproducible	reproducible	ADJ
bionys-354	41	15	bisulfite	bisulfite	NOUN
bionys-354	41	16	conversion	conversion	NOUN
bionys-354	41	17	leading	lead	VERB
bionys-354	41	18	to	to	ADP
bionys-354	41	19	unreliable	unreliable	ADJ
bionys-354	41	20	and	and	CCONJ
bionys-354	41	21	inconsistent	inconsistent	ADJ
bionys-354	41	22	results	result	NOUN
bionys-354	41	23	for	for	ADP
bionys-354	41	24	methylation	methylation	NOUN
bionys-354	41	25	levels	level	NOUN
bionys-354	41	26	(	(	PUNCT
bionys-354	41	27	tournier	tournier	X
bionys-354	41	28	et	et	PROPN
bionys-354	41	29	al	al	PROPN
bionys-354	41	30	.	.	PROPN
bionys-354	41	31	,	,	PUNCT
bionys-354	41	32	2012	2012	NUM
bionys-354	41	33	)	)	PUNCT
bionys-354	41	34	.	.	PUNCT
bionys-354	42	1	in	in	ADP
bionys-354	42	2	contrast	contrast	NOUN
bionys-354	42	3	with	with	ADP
bionys-354	42	4	ffpe	ffpe	NOUN
bionys-354	42	5	,	,	PUNCT
bionys-354	42	6	the	the	DET
bionys-354	42	7	use	use	NOUN
bionys-354	42	8	of	of	ADP
bionys-354	42	9	fresh	fresh	ADJ
bionys-354	42	10	frozen	frozen	ADJ
bionys-354	42	11	tissue	tissue	NOUN
bionys-354	42	12	(	(	PUNCT
bionys-354	42	13	ff	ff	NOUN
bionys-354	42	14	)	)	PUNCT
bionys-354	42	15	brings	bring	VERB
bionys-354	42	16	reproducible	reproducible	ADJ
bionys-354	42	17	and	and	CCONJ
bionys-354	42	18	satisfactory	satisfactory	ADJ
bionys-354	42	19	results	result	NOUN
bionys-354	42	20	,	,	PUNCT
bionys-354	42	21	owing	owe	VERB
bionys-354	42	22	to	to	ADP
bionys-354	42	23	the	the	DET
bionys-354	42	24	process	process	NOUN
bionys-354	42	25	of	of	ADP
bionys-354	42	26	cryopreservation	cryopreservation	NOUN
bionys-354	42	27	which	which	PRON
bionys-354	42	28	provides	provide	VERB
bionys-354	42	29	the	the	DET
bionys-354	42	30	adequate	adequate	ADJ
bionys-354	42	31	dna	dna	PROPN
bionys-354	42	32	preservation	preservation	NOUN
bionys-354	42	33	.	.	PUNCT
bionys-354	43	1	given	give	VERB
bionys-354	43	2	that	that	SCONJ
bionys-354	43	3	the	the	DET
bionys-354	43	4	ffpe	ffpe	NOUN
bionys-354	43	5	samples	sample	NOUN
bionys-354	43	6	consist	consist	VERB
bionys-354	43	7	of	of	ADP
bionys-354	43	8	degraded	degraded	ADJ
bionys-354	43	9	dna	dna	NOUN
bionys-354	43	10	generally	generally	ADV
bionys-354	43	11	less	less	ADJ
bionys-354	43	12	than	than	ADP
bionys-354	43	13	300	300	NUM
bionys-354	43	14	bp	bp	NOUN
bionys-354	43	15	,	,	PUNCT
bionys-354	43	16	the	the	DET
bionys-354	43	17	main	main	ADJ
bionys-354	43	18	challenge	challenge	NOUN
bionys-354	43	19	with	with	ADP
bionys-354	43	20	managing	manage	VERB
bionys-354	43	21	ffpe	ffpe	NOUN
bionys-354	43	22	samples	sample	NOUN
bionys-354	43	23	is	be	AUX
bionys-354	43	24	to	to	PART
bionys-354	43	25	provide	provide	VERB
bionys-354	43	26	an	an	DET
bionys-354	43	27	efficient	efficient	ADJ
bionys-354	43	28	cell	cell	NOUN
bionys-354	43	29	lysis	lysis	NOUN
bionys-354	43	30	which	which	PRON
bionys-354	43	31	releases	release	VERB
bionys-354	43	32	dna	dna	NOUN
bionys-354	43	33	of	of	ADP
bionys-354	43	34	sufficient	sufficient	ADJ
bionys-354	43	35	quality	quality	NOUN
bionys-354	43	36	and	and	CCONJ
bionys-354	43	37	quantity	quantity	NOUN
bionys-354	43	38	for	for	ADP
bionys-354	43	39	further	further	ADJ
bionys-354	43	40	analysis	analysis	NOUN
bionys-354	43	41	(	(	PUNCT
bionys-354	43	42	holmes	holmes	PROPN
bionys-354	43	43	et	et	PROPN
bionys-354	43	44	al	al	PROPN
bionys-354	43	45	.	.	PROPN
bionys-354	43	46	,	,	PUNCT
bionys-354	43	47	2014	2014	NUM
bionys-354	43	48	)	)	PUNCT
bionys-354	43	49	.	.	PUNCT
bionys-354	44	1	what	what	PRON
bionys-354	44	2	makes	make	VERB
bionys-354	44	3	it	it	PRON
bionys-354	44	4	even	even	ADV
bionys-354	44	5	more	more	ADV
bionys-354	44	6	difficult	difficult	ADJ
bionys-354	44	7	,	,	PUNCT
bionys-354	44	8	formalin	formalin	NOUN
bionys-354	44	9	-	-	PUNCT
bionys-354	44	10	fixation	fixation	NOUN
bionys-354	44	11	induces	induce	VERB
bionys-354	44	12	the	the	DET
bionys-354	44	13	formation	formation	NOUN
bionys-354	44	14	of	of	ADP
bionys-354	44	15	dna	dna	NOUN
bionys-354	44	16	-	-	PUNCT
bionys-354	44	17	protein	protein	NOUN
bionys-354	44	18	crosslinks	crosslink	NOUN
bionys-354	44	19	,	,	PUNCT
bionys-354	44	20	which	which	PRON
bionys-354	44	21	often	often	ADV
bionys-354	44	22	could	could	AUX
bionys-354	44	23	not	not	PART
bionys-354	44	24	be	be	AUX
bionys-354	44	25	completely	completely	ADV
bionys-354	44	26	removed	remove	VERB
bionys-354	44	27	by	by	ADP
bionys-354	44	28	common	common	ADJ
bionys-354	44	29	lysis	lysis	NOUN
bionys-354	44	30	protocols	protocol	NOUN
bionys-354	44	31	.	.	PUNCT
bionys-354	45	1	nevertheless	nevertheless	ADV
bionys-354	45	2	,	,	PUNCT
bionys-354	45	3	as	as	SCONJ
bionys-354	45	4	ffpe	ffpe	NOUN
bionys-354	45	5	samples	sample	NOUN
bionys-354	45	6	are	be	AUX
bionys-354	45	7	widely	widely	ADV
bionys-354	45	8	available	available	ADJ
bionys-354	45	9	,	,	PUNCT
bionys-354	45	10	this	this	DET
bionys-354	45	11	type	type	NOUN
bionys-354	45	12	of	of	ADP
bionys-354	45	13	tissue	tissue	NOUN
bionys-354	45	14	sample	sample	NOUN
bionys-354	45	15	is	be	AUX
bionys-354	45	16	the	the	DET
bionys-354	45	17	most	most	ADV
bionys-354	45	18	commonly	commonly	ADV
bionys-354	45	19	used	use	VERB
bionys-354	45	20	source	source	NOUN
bionys-354	45	21	for	for	ADP
bionys-354	45	22	tissue	tissue	NOUN
bionys-354	45	23	-	-	PUNCT
bionys-354	45	24	based	base	VERB
bionys-354	45	25	molecular	molecular	ADJ
bionys-354	45	26	testing	testing	NOUN
bionys-354	45	27	(	(	PUNCT
bionys-354	45	28	dietrich	dietrich	PROPN
bionys-354	45	29	et	et	PROPN
bionys-354	45	30	al	al	PROPN
bionys-354	45	31	.	.	PROPN
bionys-354	45	32	,	,	PUNCT
bionys-354	45	33	2013	2013	NUM
bionys-354	45	34	)	)	PUNCT
bionys-354	45	35	.	.	PUNCT
bionys-354	46	1	alongside	alongside	ADP
bionys-354	46	2	the	the	DET
bionys-354	46	3	low	low	ADJ
bionys-354	46	4	pricing	pricing	NOUN
bionys-354	46	5	of	of	ADP
bionys-354	46	6	long	long	ADJ
bionys-354	46	7	term	term	NOUN
bionys-354	46	8	storage	storage	NOUN
bionys-354	46	9	,	,	PUNCT
bionys-354	46	10	ffpe	ffpe	NOUN
bionys-354	46	11	is	be	AUX
bionys-354	46	12	often	often	ADV
bionys-354	46	13	the	the	DET
bionys-354	46	14	only	only	ADJ
bionys-354	46	15	available	available	ADJ
bionys-354	46	16	material	material	NOUN
bionys-354	46	17	for	for	ADP
bionys-354	46	18	retrospective	retrospective	ADJ
bionys-354	46	19	studies	study	NOUN
bionys-354	46	20	and	and	CCONJ
bionys-354	46	21	the	the	DET
bionys-354	46	22	most	most	ADV
bionys-354	46	23	important	important	ADJ
bionys-354	46	24	material	material	NOUN
bionys-354	46	25	for	for	ADP
bionys-354	46	26	standard	standard	ADJ
bionys-354	46	27	routine	routine	ADJ
bionys-354	46	28	diagnostics	diagnostic	NOUN
bionys-354	46	29	in	in	ADP
bionys-354	46	30	the	the	DET
bionys-354	46	31	era	era	NOUN
bionys-354	46	32	of	of	ADP
bionys-354	46	33	personalized	personalized	ADJ
bionys-354	46	34	medicine	medicine	NOUN
bionys-354	46	35	(	(	PUNCT
bionys-354	46	36	dietrich	dietrich	PROPN
bionys-354	46	37	et	et	PROPN
bionys-354	46	38	al	al	PROPN
bionys-354	46	39	.	.	PROPN
bionys-354	46	40	,	,	PUNCT
bionys-354	46	41	2013	2013	NUM
bionys-354	46	42	)	)	PUNCT
bionys-354	46	43	.	.	PUNCT
bionys-354	47	1	therefore	therefore	ADV
bionys-354	47	2	,	,	PUNCT
bionys-354	47	3	several	several	ADJ
bionys-354	47	4	commercially	commercially	ADV
bionys-354	47	5	available	available	ADJ
bionys-354	47	6	kits	kit	NOUN
bionys-354	47	7	for	for	ADP
bionys-354	47	8	ffpe	ffpe	NOUN
bionys-354	47	9	dna	dna	PROPN
bionys-354	47	10	isolation	isolation	NOUN
bionys-354	47	11	and	and	CCONJ
bionys-354	47	12	bisulfite	bisulfite	NOUN
bionys-354	47	13	conversion	conversion	NOUN
bionys-354	47	14	were	be	AUX
bionys-354	47	15	developed	develop	VERB
bionys-354	47	16	to	to	PART
bionys-354	47	17	ensure	ensure	VERB
bionys-354	47	18	results	result	NOUN
bionys-354	47	19	as	as	ADV
bionys-354	47	20	reliable	reliable	ADJ
bionys-354	47	21	as	as	ADP
bionys-354	47	22	possible	possible	ADJ
bionys-354	47	23	(	(	PUNCT
bionys-354	47	24	holmes	holmes	PROPN
bionys-354	47	25	et	et	PROPN
bionys-354	47	26	al	al	PROPN
bionys-354	47	27	.	.	PROPN
bionys-354	47	28	,	,	PUNCT
bionys-354	47	29	2014	2014	NUM
bionys-354	47	30	;	;	PUNCT
bionys-354	47	31	de	de	X
bionys-354	47	32	ruijter	ruijter	NOUN
bionys-354	47	33	et	et	PROPN
bionys-354	47	34	al	al	PROPN
bionys-354	47	35	.	.	PROPN
bionys-354	47	36	,	,	PUNCT
bionys-354	47	37	2015	2015	NUM
bionys-354	47	38	;	;	PUNCT
bionys-354	47	39	ludgate	ludgate	PROPN
bionys-354	47	40	et	et	PROPN
bionys-354	47	41	al	al	PROPN
bionys-354	47	42	.	.	PROPN
bionys-354	47	43	,	,	PUNCT
bionys-354	47	44	2017	2017	NUM
bionys-354	47	45	;	;	PUNCT
bionys-354	47	46	kint	kint	NOUN
bionys-354	47	47	et	et	PROPN
bionys-354	47	48	al	al	PROPN
bionys-354	47	49	.	.	PROPN
bionys-354	47	50	,	,	PUNCT
bionys-354	47	51	2018	2018	NUM
bionys-354	47	52	)	)	PUNCT
bionys-354	47	53	.	.	PUNCT
bionys-354	48	1	this	this	DET
bionys-354	48	2	study	study	NOUN
bionys-354	48	3	aimed	aim	VERB
bionys-354	48	4	to	to	PART
bionys-354	48	5	define	define	VERB
bionys-354	48	6	optimal	optimal	ADJ
bionys-354	48	7	pcr	pcr	ADJ
bionys-354	48	8	conditions	condition	NOUN
bionys-354	48	9	for	for	ADP
bionys-354	48	10	evaluation	evaluation	NOUN
bionys-354	48	11	of	of	ADP
bionys-354	48	12	mgmt	mgmt	PROPN
bionys-354	48	13	promoter	promoter	NOUN
bionys-354	48	14	methylation	methylation	NOUN
bionys-354	48	15	status	status	NOUN
bionys-354	48	16	in	in	ADP
bionys-354	48	17	the	the	DET
bionys-354	48	18	ffpe	ffpe	NOUN
bionys-354	48	19	sample	sample	NOUN
bionys-354	48	20	obtained	obtain	VERB
bionys-354	48	21	from	from	ADP
bionys-354	48	22	gbm	gbm	PROPN
bionys-354	48	23	patient	patient	NOUN
bionys-354	48	24	,	,	PUNCT
bionys-354	48	25	considering	consider	VERB
bionys-354	48	26	the	the	DET
bionys-354	48	27	recommendations	recommendation	NOUN
bionys-354	48	28	from	from	ADP
bionys-354	48	29	the	the	DET
bionys-354	48	30	previous	previous	ADJ
bionys-354	48	31	studies	study	NOUN
bionys-354	48	32	(	(	PUNCT
bionys-354	48	33	dietrich	dietrich	PROPN
bionys-354	48	34	et	et	PROPN
bionys-354	48	35	al	al	PROPN
bionys-354	48	36	.	.	PROPN
bionys-354	48	37	,	,	PUNCT
bionys-354	48	38	2013	2013	NUM
bionys-354	48	39	)	)	PUNCT
bionys-354	48	40	.	.	PUNCT
bionys-354	49	1	also	also	ADV
bionys-354	49	2	,	,	PUNCT
bionys-354	49	3	to	to	PART
bionys-354	49	4	investigate	investigate	VERB
bionys-354	49	5	the	the	DET
bionys-354	49	6	validity	validity	NOUN
bionys-354	49	7	of	of	ADP
bionys-354	49	8	the	the	DET
bionys-354	49	9	results	result	NOUN
bionys-354	49	10	,	,	PUNCT
bionys-354	49	11	they	they	PRON
bionys-354	49	12	were	be	AUX
bionys-354	49	13	compared	compare	VERB
bionys-354	49	14	with	with	ADP
bionys-354	49	15	mgmt	mgmt	NOUN
bionys-354	49	16	promoter	promoter	NOUN
bionys-354	49	17	methylation	methylation	NOUN
bionys-354	49	18	status	status	NOUN
bionys-354	49	19	obtained	obtain	VERB
bionys-354	49	20	from	from	ADP
bionys-354	49	21	the	the	DET
bionys-354	49	22	ff	ff	NOUN
bionys-354	49	23	sample	sample	NOUN
bionys-354	49	24	of	of	ADP
bionys-354	49	25	the	the	DET
bionys-354	49	26	same	same	ADJ
bionys-354	49	27	gbm	gbm	NOUN
bionys-354	49	28	patient	patient	NOUN
bionys-354	49	29	.	.	PUNCT
bionys-354	50	1	material	material	NOUN
bionys-354	50	2	and	and	CCONJ
bionys-354	50	3	methods	method	NOUN
bionys-354	50	4	patient	patient	ADJ
bionys-354	50	5	and	and	CCONJ
bionys-354	50	6	tumor	tumor	NOUN
bionys-354	50	7	specimens	specimen	NOUN
bionys-354	50	8	both	both	CCONJ
bionys-354	50	9	ff	ff	NOUN
bionys-354	50	10	and	and	CCONJ
bionys-354	50	11	ffpe	ffpe	ADJ
bionys-354	50	12	tissue	tissue	NOUN
bionys-354	50	13	tumor	tumor	NOUN
bionys-354	50	14	specimens	specimen	NOUN
bionys-354	50	15	were	be	AUX
bionys-354	50	16	collected	collect	VERB
bionys-354	50	17	from	from	ADP
bionys-354	50	18	the	the	DET
bionys-354	50	19	gbm	gbm	NOUN
bionys-354	50	20	patient	patient	NOUN
bionys-354	50	21	(	(	PUNCT
bionys-354	50	22	male	male	NOUN
bionys-354	50	23	,	,	PUNCT
bionys-354	50	24	63	63	NUM
bionys-354	50	25	years	year	NOUN
bionys-354	50	26	old	old	ADJ
bionys-354	50	27	)	)	PUNCT
bionys-354	50	28	operated	operate	VERB
bionys-354	50	29	on	on	ADP
bionys-354	50	30	at	at	ADP
bionys-354	50	31	the	the	DET
bionys-354	50	32	neurosurgery	neurosurgery	ADJ
bionys-354	50	33	clinic	clinic	NOUN
bionys-354	50	34	(	(	PUNCT
bionys-354	50	35	the	the	DET
bionys-354	50	36	biologica	biologica	PROPN
bionys-354	50	37	nyssana	nyssana	PROPN
bionys-354	50	38	●	●	PUNCT
bionys-354	50	39	11	11	NUM
bionys-354	50	40	(	(	PUNCT
bionys-354	50	41	2	2	NUM
bionys-354	50	42	)	)	PUNCT
bionys-354	50	43	december	december	PROPN
bionys-354	50	44	2020	2020	NUM
bionys-354	50	45	:	:	PUNCT
bionys-354	50	46	199	199	NUM
bionys-354	50	47	-	-	SYM
bionys-354	50	48	147	147	NUM
bionys-354	50	49	jovanović	jovanović	NOUN
bionys-354	50	50	et	et	PROPN
bionys-354	50	51	al	al	PROPN
bionys-354	50	52	.	.	PUNCT
bionys-354	51	1	●	●	PUNCT
bionys-354	51	2	optimizing	optimize	VERB
bionys-354	51	3	conditions	condition	NOUN
bionys-354	51	4	for	for	ADP
bionys-354	51	5	mgmt	mgmt	NOUN
bionys-354	51	6	promoter	promoter	NOUN
bionys-354	51	7	methylation	methylation	NOUN
bionys-354	51	8	status	status	NOUN
bionys-354	51	9	analysis	analysis	NOUN
bionys-354	51	10	in	in	ADP
bionys-354	51	11	glioblastoma	glioblastoma	NOUN
bionys-354	51	12	ffpe	ffpe	NOUN
bionys-354	51	13	samples	sample	NOUN
bionys-354	51	14	141	141	NUM
bionys-354	51	15	biologica	biologica	NOUN
bionys-354	51	16	nyssana	nyssana	PROPN
bionys-354	52	1	●	●	PUNCT
bionys-354	52	2	11	11	NUM
bionys-354	52	3	(	(	PUNCT
bionys-354	52	4	2	2	NUM
bionys-354	52	5	)	)	PUNCT
bionys-354	52	6	december	december	PROPN
bionys-354	52	7	2020	2020	NUM
bionys-354	52	8	:	:	PUNCT
bionys-354	52	9	199	199	NUM
bionys-354	52	10	-	-	SYM
bionys-354	52	11	147	147	NUM
bionys-354	52	12	jovanović	jovanović	NOUN
bionys-354	52	13	et	et	PROPN
bionys-354	52	14	al	al	PROPN
bionys-354	52	15	.	.	PUNCT
bionys-354	53	1	●	●	PUNCT
bionys-354	53	2	optimizing	optimize	VERB
bionys-354	53	3	conditions	condition	NOUN
bionys-354	53	4	for	for	ADP
bionys-354	53	5	mgmt	mgmt	NOUN
bionys-354	53	6	promoter	promoter	NOUN
bionys-354	53	7	methylation	methylation	NOUN
bionys-354	53	8	status	status	NOUN
bionys-354	53	9	analysis	analysis	NOUN
bionys-354	53	10	in	in	ADP
bionys-354	53	11	glioblastoma	glioblastoma	NOUN
bionys-354	53	12	ffpe	ffpe	NOUN
bionys-354	53	13	samples	sample	NOUN
bionys-354	53	14	clinical	clinical	ADJ
bionys-354	53	15	centre	centre	NOUN
bionys-354	53	16	of	of	ADP
bionys-354	53	17	niš	niš	NOUN
bionys-354	53	18	,	,	PUNCT
bionys-354	53	19	serbia	serbia	PROPN
bionys-354	53	20	)	)	PUNCT
bionys-354	53	21	in	in	ADP
bionys-354	53	22	2013	2013	NUM
bionys-354	53	23	.	.	PUNCT
bionys-354	54	1	the	the	DET
bionys-354	54	2	patient	patient	ADJ
bionys-354	54	3	underwent	undergo	VERB
bionys-354	54	4	total	total	ADJ
bionys-354	54	5	resection	resection	NOUN
bionys-354	54	6	of	of	ADP
bionys-354	54	7	the	the	DET
bionys-354	54	8	tumor	tumor	NOUN
bionys-354	54	9	and	and	CCONJ
bionys-354	54	10	had	have	VERB
bionys-354	54	11	a	a	DET
bionys-354	54	12	karnofsky	karnofsky	ADJ
bionys-354	54	13	score	score	NOUN
bionys-354	54	14	of	of	ADP
bionys-354	54	15	≥80	≥80	NUM
bionys-354	54	16	%	%	NOUN
bionys-354	54	17	.	.	PUNCT
bionys-354	55	1	the	the	DET
bionys-354	55	2	diagnosis	diagnosis	NOUN
bionys-354	55	3	of	of	ADP
bionys-354	55	4	glioblastoma	glioblastoma	NOUN
bionys-354	55	5	who	who	PRON
bionys-354	55	6	grade	grade	VERB
bionys-354	55	7	iv	iv	NUM
bionys-354	55	8	was	be	AUX
bionys-354	55	9	confirmed	confirm	VERB
bionys-354	55	10	by	by	ADP
bionys-354	55	11	an	an	DET
bionys-354	55	12	expert	expert	ADJ
bionys-354	55	13	neuropathologist	neuropathologist	NOUN
bionys-354	55	14	(	(	PUNCT
bionys-354	55	15	n.v	n.v	PROPN
bionys-354	55	16	and	and	CCONJ
bionys-354	55	17	m.k	m.k	PROPN
bionys-354	55	18	)	)	PUNCT
bionys-354	55	19	.	.	PUNCT
bionys-354	56	1	the	the	DET
bionys-354	56	2	written	write	VERB
bionys-354	56	3	informed	inform	VERB
bionys-354	56	4	consent	consent	NOUN
bionys-354	56	5	of	of	ADP
bionys-354	56	6	study	study	NOUN
bionys-354	56	7	participation	participation	NOUN
bionys-354	56	8	was	be	AUX
bionys-354	56	9	obtained	obtain	VERB
bionys-354	56	10	from	from	ADP
bionys-354	56	11	the	the	DET
bionys-354	56	12	gbm	gbm	NOUN
bionys-354	56	13	patient	patient	NOUN
bionys-354	56	14	.	.	PUNCT
bionys-354	57	1	the	the	DET
bionys-354	57	2	ethics	ethics	PROPN
bionys-354	57	3	committee	committee	PROPN
bionys-354	57	4	of	of	ADP
bionys-354	57	5	the	the	DET
bionys-354	57	6	faculty	faculty	NOUN
bionys-354	57	7	of	of	ADP
bionys-354	57	8	medicine	medicine	NOUN
bionys-354	57	9	,	,	PUNCT
bionys-354	57	10	niš	niš	NOUN
bionys-354	57	11	,	,	PUNCT
bionys-354	57	12	serbia	serbia	PROPN
bionys-354	57	13	,	,	PUNCT
bionys-354	57	14	approved	approve	VERB
bionys-354	57	15	the	the	DET
bionys-354	57	16	informed	informed	ADJ
bionys-354	57	17	consent	consent	NOUN
bionys-354	57	18	form	form	NOUN
bionys-354	57	19	and	and	CCONJ
bionys-354	57	20	study	study	NOUN
bionys-354	57	21	protocol	protocol	NOUN
bionys-354	57	22	(	(	PUNCT
bionys-354	57	23	01	01	NUM
bionys-354	57	24	-	-	PUNCT
bionys-354	57	25	211310	211310	NUM
bionys-354	57	26	)	)	PUNCT
bionys-354	57	27	.	.	PUNCT
bionys-354	58	1	dna	dna	PROPN
bionys-354	58	2	isolation	isolation	NOUN
bionys-354	58	3	and	and	CCONJ
bionys-354	58	4	bisulfite	bisulfite	NOUN
bionys-354	58	5	conversion	conversion	NOUN
bionys-354	58	6	extraction	extraction	NOUN
bionys-354	58	7	of	of	ADP
bionys-354	58	8	genomic	genomic	ADJ
bionys-354	58	9	dna	dna	PROPN
bionys-354	58	10	was	be	AUX
bionys-354	58	11	performed	perform	VERB
bionys-354	58	12	using	use	VERB
bionys-354	58	13	qiaamp	qiaamp	PROPN
bionys-354	58	14	®	®	PROPN
bionys-354	58	15	dna	dna	PROPN
bionys-354	58	16	mini	mini	NOUN
bionys-354	58	17	kit	kit	NOUN
bionys-354	58	18	(	(	PUNCT
bionys-354	58	19	qiagen	qiagen	PROPN
bionys-354	58	20	,	,	PUNCT
bionys-354	58	21	hilden	hilden	PROPN
bionys-354	58	22	,	,	PUNCT
bionys-354	58	23	germany	germany	PROPN
bionys-354	58	24	)	)	PUNCT
bionys-354	58	25	from	from	ADP
bionys-354	58	26	25	25	NUM
bionys-354	58	27	mg	mg	NOUN
bionys-354	58	28	of	of	ADP
bionys-354	58	29	ff	ff	NOUN
bionys-354	58	30	sample	sample	NOUN
bionys-354	58	31	and	and	CCONJ
bionys-354	58	32	qiaamp	qiaamp	VERB
bionys-354	58	33	dna	dna	PROPN
bionys-354	58	34	ffpe	ffpe	NOUN
bionys-354	58	35	tissue	tissue	NOUN
bionys-354	58	36	kit	kit	NOUN
bionys-354	58	37	(	(	PUNCT
bionys-354	58	38	qiagen	qiagen	PROPN
bionys-354	58	39	,	,	PUNCT
bionys-354	58	40	hilden	hilden	PROPN
bionys-354	58	41	,	,	PUNCT
bionys-354	58	42	germany	germany	PROPN
bionys-354	58	43	,	,	PUNCT
bionys-354	58	44	catalogue	catalogue	NOUN
bionys-354	58	45	no	no	NOUN
bionys-354	58	46	.	.	NOUN
bionys-354	59	1	56404	56404	NUM
bionys-354	59	2	)	)	PUNCT
bionys-354	60	1	from	from	ADP
bionys-354	60	2	8	8	NUM
bionys-354	60	3	freshly	freshly	ADV
bionys-354	60	4	cut	cut	VERB
bionys-354	60	5	sections	section	NOUN
bionys-354	60	6	with	with	ADP
bionys-354	60	7	a	a	DET
bionys-354	60	8	thickness	thickness	NOUN
bionys-354	60	9	of	of	ADP
bionys-354	60	10	10	10	NUM
bionys-354	60	11	µm	µm	NOUN
bionys-354	60	12	from	from	ADP
bionys-354	60	13	ffpe	ffpe	NOUN
bionys-354	60	14	sample	sample	NOUN
bionys-354	60	15	.	.	PUNCT
bionys-354	61	1	a	a	DET
bionys-354	61	2	total	total	NOUN
bionys-354	61	3	of	of	ADP
bionys-354	61	4	2	2	NUM
bionys-354	61	5	µg	µg	NOUN
bionys-354	61	6	of	of	ADP
bionys-354	61	7	genomic	genomic	ADJ
bionys-354	61	8	dna	dna	PROPN
bionys-354	61	9	was	be	AUX
bionys-354	61	10	modified	modify	VERB
bionys-354	61	11	by	by	ADP
bionys-354	61	12	sodium	sodium	NOUN
bionys-354	61	13	bisulfite	bisulfite	NOUN
bionys-354	61	14	using	use	VERB
bionys-354	61	15	epitect	epitect	ADJ
bionys-354	61	16	®	®	NOUN
bionys-354	61	17	bisulfite	bisulfite	NOUN
bionys-354	61	18	kit	kit	NOUN
bionys-354	61	19	(	(	PUNCT
bionys-354	61	20	qiagen	qiagen	PROPN
bionys-354	61	21	,	,	PUNCT
bionys-354	61	22	hilden	hilden	PROPN
bionys-354	61	23	,	,	PUNCT
bionys-354	61	24	germany	germany	PROPN
bionys-354	61	25	)	)	PUNCT
bionys-354	61	26	for	for	ADP
bionys-354	61	27	the	the	DET
bionys-354	61	28	ff	ff	PROPN
bionys-354	61	29	dna	dna	PROPN
bionys-354	61	30	sample	sample	NOUN
bionys-354	61	31	and	and	CCONJ
bionys-354	61	32	epitect	epitect	ADJ
bionys-354	61	33	plus	plus	CCONJ
bionys-354	61	34	ffpe	ffpe	NOUN
bionys-354	61	35	bisulfite	bisulfite	NOUN
bionys-354	61	36	kit	kit	NOUN
bionys-354	61	37	(	(	PUNCT
bionys-354	61	38	qiagen	qiagen	PROPN
bionys-354	61	39	,	,	PUNCT
bionys-354	61	40	hilden	hilden	PROPN
bionys-354	61	41	,	,	PUNCT
bionys-354	61	42	germany	germany	PROPN
bionys-354	61	43	,	,	PUNCT
bionys-354	61	44	catalogue	catalogue	NOUN
bionys-354	61	45	no	no	NOUN
bionys-354	61	46	.	.	NOUN
bionys-354	62	1	59144	59144	NUM
bionys-354	62	2	)	)	PUNCT
bionys-354	63	1	for	for	ADP
bionys-354	63	2	ffpe	ffpe	ADJ
bionys-354	63	3	dna	dna	PROPN
bionys-354	63	4	sample	sample	NOUN
bionys-354	63	5	.	.	PUNCT
bionys-354	64	1	biospec	biospec	NOUN
bionys-354	64	2	–	–	PUNCT
bionys-354	64	3	nano	nano	ADJ
bionys-354	64	4	uv	uv	NOUN
bionys-354	64	5	–	–	PUNCT
bionys-354	64	6	vis	vis	X
bionys-354	64	7	spectrophotometer	spectrophotometer	X
bionys-354	64	8	(	(	PUNCT
bionys-354	64	9	shimadzu	shimadzu	PROPN
bionys-354	64	10	,	,	PUNCT
bionys-354	64	11	kyoto	kyoto	PROPN
bionys-354	64	12	,	,	PUNCT
bionys-354	64	13	japan	japan	PROPN
bionys-354	64	14	)	)	PUNCT
bionys-354	64	15	was	be	AUX
bionys-354	64	16	utilized	utilize	VERB
bionys-354	64	17	for	for	ADP
bionys-354	64	18	the	the	DET
bionys-354	64	19	determination	determination	NOUN
bionys-354	64	20	of	of	ADP
bionys-354	64	21	quantity	quantity	NOUN
bionys-354	64	22	and	and	CCONJ
bionys-354	64	23	quality	quality	NOUN
bionys-354	64	24	of	of	ADP
bionys-354	64	25	isolated	isolated	ADJ
bionys-354	64	26	dna	dna	NOUN
bionys-354	64	27	and	and	CCONJ
bionys-354	64	28	bisulfite	bisulfite	NOUN
bionys-354	64	29	converted	convert	VERB
bionys-354	64	30	samples	sample	NOUN
bionys-354	64	31	.	.	PUNCT
bionys-354	65	1	isolated	isolate	VERB
bionys-354	65	2	dna	dna	NOUN
bionys-354	65	3	samples	sample	NOUN
bionys-354	65	4	were	be	AUX
bionys-354	65	5	inspected	inspect	VERB
bionys-354	65	6	for	for	ADP
bionys-354	65	7	degradation	degradation	NOUN
bionys-354	65	8	and	and	CCONJ
bionys-354	65	9	visualized	visualize	VERB
bionys-354	65	10	by	by	ADP
bionys-354	65	11	running	run	VERB
bionys-354	65	12	the	the	DET
bionys-354	65	13	dna	dna	PROPN
bionys-354	65	14	samples	sample	NOUN
bionys-354	65	15	on	on	ADP
bionys-354	65	16	2	2	NUM
bionys-354	65	17	%	%	NOUN
bionys-354	65	18	agarose	agarose	X
bionys-354	65	19	gel	gel	NOUN
bionys-354	65	20	.	.	PUNCT
bionys-354	66	1	methylation	methylation	NOUN
bionys-354	66	2	-	-	PUNCT
bionys-354	66	3	specific	specific	ADJ
bionys-354	66	4	polymerase	polymerase	NOUN
bionys-354	66	5	chain	chain	NOUN
bionys-354	66	6	reaction	reaction	NOUN
bionys-354	66	7	(	(	PUNCT
bionys-354	66	8	msp	msp	PROPN
bionys-354	66	9	)	)	PUNCT
bionys-354	66	10	all	all	PRON
bionys-354	66	11	of	of	ADP
bionys-354	66	12	the	the	DET
bionys-354	66	13	msp	msp	PROPN
bionys-354	66	14	reactions	reaction	NOUN
bionys-354	66	15	were	be	AUX
bionys-354	66	16	carried	carry	VERB
bionys-354	66	17	out	out	ADP
bionys-354	66	18	in	in	ADP
bionys-354	66	19	a	a	DET
bionys-354	66	20	total	total	ADJ
bionys-354	66	21	volume	volume	NOUN
bionys-354	66	22	of	of	ADP
bionys-354	66	23	20	20	NUM
bionys-354	66	24	µl	µl	NOUN
bionys-354	66	25	containing	contain	VERB
bionys-354	66	26	0.2	0.2	NUM
bionys-354	66	27	μm	μm	NUM
bionys-354	66	28	dntp	dntp	NOUN
bionys-354	66	29	mix	mix	NOUN
bionys-354	66	30	,	,	PUNCT
bionys-354	66	31	1	1	NUM
bionys-354	66	32	×	×	NOUN
bionys-354	66	33	pcr	pcr	NOUN
bionys-354	66	34	buffer	buffer	NOUN
bionys-354	66	35	with	with	ADP
bionys-354	66	36	1.5	1.5	NUM
bionys-354	66	37	mm	mm	NOUN
bionys-354	66	38	mgcl2	mgcl2	PROPN
bionys-354	66	39	(	(	PUNCT
bionys-354	66	40	qiagen	qiagen	PROPN
bionys-354	66	41	,	,	PUNCT
bionys-354	66	42	hilden	hilden	PROPN
bionys-354	66	43	,	,	PUNCT
bionys-354	66	44	germany	germany	PROPN
bionys-354	66	45	)	)	PUNCT
bionys-354	66	46	and	and	CCONJ
bionys-354	66	47	10	10	NUM
bionys-354	66	48	pm	pm	NOUN
bionys-354	66	49	of	of	ADP
bionys-354	66	50	appropriate	appropriate	ADJ
bionys-354	66	51	forward	forward	ADV
bionys-354	66	52	and	and	CCONJ
bionys-354	66	53	reverse	reverse	ADJ
bionys-354	66	54	primer	primer	NOUN
bionys-354	66	55	(	(	PUNCT
bionys-354	66	56	tab	tab	NOUN
bionys-354	66	57	.	.	NOUN
bionys-354	66	58	1	1	NUM
bionys-354	66	59	)	)	PUNCT
bionys-354	66	60	.	.	PUNCT
bionys-354	67	1	amplification	amplification	NOUN
bionys-354	67	2	reactions	reaction	NOUN
bionys-354	67	3	were	be	AUX
bionys-354	67	4	performed	perform	VERB
bionys-354	67	5	in	in	ADP
bionys-354	67	6	a	a	DET
bionys-354	67	7	mastercycler	mastercycler	ADJ
bionys-354	67	8	gradient	gradient	NOUN
bionys-354	67	9	(	(	PUNCT
bionys-354	67	10	eppendorf	eppendorf	PROPN
bionys-354	67	11	)	)	PUNCT
bionys-354	67	12	using	use	VERB
bionys-354	67	13	the	the	DET
bionys-354	67	14	following	follow	VERB
bionys-354	67	15	program	program	NOUN
bionys-354	67	16	:	:	PUNCT
bionys-354	68	1	95	95	NUM
bionys-354	68	2	°	°	NUM
bionys-354	68	3	c	c	NOUN
bionys-354	68	4	for	for	ADP
bionys-354	68	5	15	15	NUM
bionys-354	68	6	min	min	NOUN
bionys-354	68	7	,	,	PUNCT
bionys-354	68	8	then	then	ADV
bionys-354	68	9	35	35	NUM
bionys-354	68	10	cycles	cycle	NOUN
bionys-354	68	11	of	of	ADP
bionys-354	68	12	95	95	NUM
bionys-354	68	13	°	°	ADP
bionys-354	68	14	c	c	NOUN
bionys-354	68	15	for	for	ADP
bionys-354	68	16	50	50	NUM
bionys-354	68	17	s	s	NOUN
bionys-354	68	18	,	,	PUNCT
bionys-354	68	19	59	59	NUM
bionys-354	68	20	°	°	NUM
bionys-354	68	21	c	c	NOUN
bionys-354	68	22	for	for	ADP
bionys-354	68	23	50	50	NUM
bionys-354	68	24	s	s	NOUN
bionys-354	68	25	and	and	CCONJ
bionys-354	68	26	72	72	NUM
bionys-354	68	27	°	°	NUM
bionys-354	68	28	c	c	NOUN
bionys-354	68	29	for	for	ADP
bionys-354	68	30	50	50	NUM
bionys-354	68	31	s	s	NOUN
bionys-354	68	32	,	,	PUNCT
bionys-354	68	33	and	and	CCONJ
bionys-354	68	34	a	a	DET
bionys-354	68	35	final	final	ADJ
bionys-354	68	36	extension	extension	NOUN
bionys-354	68	37	at	at	ADP
bionys-354	68	38	72	72	NUM
bionys-354	68	39	°	°	ADP
bionys-354	68	40	c	c	NOUN
bionys-354	68	41	for	for	ADP
bionys-354	68	42	10	10	NUM
bionys-354	68	43	min	min	NOUN
bionys-354	68	44	.	.	PROPN
bionys-354	68	45	msp	msp	PROPN
bionys-354	68	46	optimization	optimization	NOUN
bionys-354	68	47	reactions	reaction	NOUN
bionys-354	68	48	with	with	ADP
bionys-354	68	49	four	four	NUM
bionys-354	68	50	different	different	ADJ
bionys-354	68	51	amounts	amount	NOUN
bionys-354	68	52	of	of	ADP
bionys-354	68	53	bisulfite	bisulfite	NOUN
bionys-354	68	54	-	-	PUNCT
bionys-354	68	55	converted	convert	VERB
bionys-354	68	56	template	template	NOUN
bionys-354	68	57	dna	dna	NOUN
bionys-354	68	58	(	(	PUNCT
bionys-354	68	59	31.25	31.25	NUM
bionys-354	68	60	ng	ng	PROPN
bionys-354	68	61	,	,	PUNCT
bionys-354	68	62	62.5	62.5	NUM
bionys-354	68	63	ng	ng	PROPN
bionys-354	68	64	,	,	PUNCT
bionys-354	68	65	125	125	NUM
bionys-354	68	66	ng	ng	PROPN
bionys-354	68	67	,	,	PUNCT
bionys-354	68	68	and	and	CCONJ
bionys-354	68	69	250	250	NUM
bionys-354	68	70	ng	ng	NOUN
bionys-354	68	71	)	)	PUNCT
bionys-354	68	72	in	in	ADP
bionys-354	68	73	combination	combination	NOUN
bionys-354	68	74	with	with	ADP
bionys-354	68	75	1u	1u	NUM
bionys-354	68	76	and	and	CCONJ
bionys-354	68	77	4u	4u	NOUN
bionys-354	68	78	hotstartaq	hotstartaq	NOUN
bionys-354	68	79	dna	dna	NOUN
bionys-354	68	80	polymerase	polymerase	NOUN
bionys-354	68	81	(	(	PUNCT
bionys-354	68	82	qiagen	qiagen	PROPN
bionys-354	68	83	,	,	PUNCT
bionys-354	68	84	hilden	hilden	PROPN
bionys-354	68	85	,	,	PUNCT
bionys-354	68	86	germany	germany	PROPN
bionys-354	68	87	,	,	PUNCT
bionys-354	68	88	catalogue	catalogue	NOUN
bionys-354	68	89	no	no	NOUN
bionys-354	68	90	.	.	NOUN
bionys-354	68	91	203203	203203	NUM
bionys-354	68	92	)	)	PUNCT
bionys-354	68	93	were	be	AUX
bionys-354	68	94	conducted	conduct	VERB
bionys-354	68	95	according	accord	VERB
bionys-354	68	96	to	to	ADP
bionys-354	68	97	suggestions	suggestion	NOUN
bionys-354	68	98	from	from	ADP
bionys-354	68	99	the	the	DET
bionys-354	68	100	previous	previous	ADJ
bionys-354	68	101	study	study	NOUN
bionys-354	68	102	(	(	PUNCT
bionys-354	68	103	dietrich	dietrich	PROPN
bionys-354	68	104	et	et	PROPN
bionys-354	68	105	al	al	PROPN
bionys-354	68	106	.	.	PROPN
bionys-354	68	107	,	,	PUNCT
bionys-354	68	108	2013	2013	NUM
bionys-354	68	109	)	)	PUNCT
bionys-354	68	110	.	.	PUNCT
bionys-354	69	1	following	follow	VERB
bionys-354	69	2	the	the	DET
bionys-354	69	3	determination	determination	NOUN
bionys-354	69	4	of	of	ADP
bionys-354	69	5	template	template	NOUN
bionys-354	69	6	dna	dna	NOUN
bionys-354	69	7	optimal	optimal	ADJ
bionys-354	69	8	concentration	concentration	NOUN
bionys-354	69	9	,	,	PUNCT
bionys-354	69	10	another	another	DET
bionys-354	69	11	set	set	NOUN
bionys-354	69	12	of	of	ADP
bionys-354	69	13	optimization	optimization	NOUN
bionys-354	69	14	reactions	reaction	NOUN
bionys-354	69	15	was	be	AUX
bionys-354	69	16	designed	design	VERB
bionys-354	69	17	regarding	regard	VERB
bionys-354	69	18	the	the	DET
bionys-354	69	19	investigation	investigation	NOUN
bionys-354	69	20	of	of	ADP
bionys-354	69	21	the	the	DET
bionys-354	69	22	optimal	optimal	ADJ
bionys-354	69	23	concentration	concentration	NOUN
bionys-354	69	24	of	of	ADP
bionys-354	69	25	hotstartaq	hotstartaq	NOUN
bionys-354	69	26	polymerase	polymerase	NOUN
bionys-354	69	27	for	for	ADP
bionys-354	69	28	successful	successful	ADJ
bionys-354	69	29	mgmt	mgmt	PROPN
bionys-354	69	30	msp	msp	PROPN
bionys-354	69	31	reactions	reaction	NOUN
bionys-354	69	32	using	use	VERB
bionys-354	69	33	ffpe	ffpe	PROPN
bionys-354	69	34	dna	dna	PROPN
bionys-354	69	35	isolates	isolate	NOUN
bionys-354	69	36	.	.	PUNCT
bionys-354	70	1	therefore	therefore	ADV
bionys-354	70	2	,	,	PUNCT
bionys-354	70	3	msp	msp	PROPN
bionys-354	70	4	reactions	reaction	NOUN
bionys-354	70	5	with	with	ADP
bionys-354	70	6	1u	1u	NUM
bionys-354	70	7	,	,	PUNCT
bionys-354	70	8	2u	2u	NOUN
bionys-354	70	9	,	,	PUNCT
bionys-354	70	10	and	and	CCONJ
bionys-354	70	11	4u	4u	NOUN
bionys-354	70	12	of	of	ADP
bionys-354	70	13	hotstartaq	hotstartaq	PROPN
bionys-354	70	14	polymerase	polymerase	NOUN
bionys-354	70	15	and	and	CCONJ
bionys-354	70	16	unmethylated	unmethylate	VERB
bionys-354	70	17	set	set	NOUN
bionys-354	70	18	of	of	ADP
bionys-354	70	19	mgmt	mgmt	NOUN
bionys-354	70	20	primers	primer	NOUN
bionys-354	70	21	were	be	AUX
bionys-354	70	22	conducted	conduct	VERB
bionys-354	70	23	simultaneously	simultaneously	ADV
bionys-354	70	24	with	with	ADP
bionys-354	70	25	msp	msp	PROPN
bionys-354	70	26	reaction	reaction	NOUN
bionys-354	70	27	including	include	VERB
bionys-354	70	28	template	template	NOUN
bionys-354	70	29	dna	dna	PROPN
bionys-354	70	30	obtained	obtain	VERB
bionys-354	70	31	from	from	ADP
bionys-354	70	32	the	the	DET
bionys-354	70	33	ff	ff	NOUN
bionys-354	70	34	sample	sample	NOUN
bionys-354	70	35	and	and	CCONJ
bionys-354	70	36	1u	1u	NUM
bionys-354	70	37	of	of	ADP
bionys-354	70	38	hotstartaq	hotstartaq	PROPN
bionys-354	70	39	polymerase	polymerase	NOUN
bionys-354	70	40	.	.	PUNCT
bionys-354	71	1	for	for	ADP
bionys-354	71	2	comparison	comparison	NOUN
bionys-354	71	3	of	of	ADP
bionys-354	71	4	methylation	methylation	NOUN
bionys-354	71	5	level	level	NOUN
bionys-354	71	6	evaluation	evaluation	NOUN
bionys-354	71	7	between	between	ADP
bionys-354	71	8	ffpe	ffpe	NOUN
bionys-354	71	9	and	and	CCONJ
bionys-354	71	10	ff	ff	ADJ
bionys-354	71	11	dna	dna	PROPN
bionys-354	71	12	isolates	isolate	NOUN
bionys-354	71	13	,	,	PUNCT
bionys-354	71	14	another	another	DET
bionys-354	71	15	set	set	NOUN
bionys-354	71	16	of	of	ADP
bionys-354	71	17	msp	msp	PROPN
bionys-354	71	18	reactions	reaction	NOUN
bionys-354	71	19	was	be	AUX
bionys-354	71	20	carried	carry	VERB
bionys-354	71	21	out	out	ADP
bionys-354	71	22	using	use	VERB
bionys-354	71	23	both	both	DET
bionys-354	71	24	sets	set	NOUN
bionys-354	71	25	of	of	ADP
bionys-354	71	26	mgmt	mgmt	NOUN
bionys-354	71	27	primers	primer	NOUN
bionys-354	71	28	and	and	CCONJ
bionys-354	71	29	ffpe	ffpe	NOUN
bionys-354	71	30	and	and	CCONJ
bionys-354	71	31	ff	ff	ADJ
bionys-354	71	32	isolates	isolate	NOUN
bionys-354	71	33	as	as	ADP
bionys-354	71	34	dna	dna	PROPN
bionys-354	71	35	templates	template	NOUN
bionys-354	71	36	,	,	PUNCT
bionys-354	71	37	followed	follow	VERB
bionys-354	71	38	by	by	ADP
bionys-354	71	39	agarose	agarose	NOUN
bionys-354	71	40	gel	gel	NOUN
bionys-354	71	41	electrophoresis	electrophoresis	NOUN
bionys-354	71	42	.	.	PUNCT
bionys-354	72	1	msp	msp	PROPN
bionys-354	72	2	reactions	reaction	NOUN
bionys-354	72	3	using	use	VERB
bionys-354	72	4	ffpederived	ffpederived	ADJ
bionys-354	72	5	bisulfite	bisulfite	NOUN
bionys-354	72	6	-	-	PUNCT
bionys-354	72	7	converted	convert	VERB
bionys-354	72	8	template	template	NOUN
bionys-354	72	9	dna	dna	NOUN
bionys-354	72	10	were	be	AUX
bionys-354	72	11	conducted	conduct	VERB
bionys-354	72	12	in	in	ADP
bionys-354	72	13	duplicate	duplicate	NOUN
bionys-354	72	14	.	.	PUNCT
bionys-354	73	1	analysis	analysis	NOUN
bionys-354	73	2	of	of	ADP
bionys-354	73	3	methylation	methylation	NOUN
bionys-354	73	4	data	datum	NOUN
bionys-354	73	5	after	after	ADP
bionys-354	73	6	each	each	DET
bionys-354	73	7	optimization	optimization	NOUN
bionys-354	73	8	,	,	PUNCT
bionys-354	73	9	msp	msp	PROPN
bionys-354	73	10	gel	gel	NOUN
bionys-354	73	11	images	image	NOUN
bionys-354	73	12	were	be	AUX
bionys-354	73	13	subjected	subject	VERB
bionys-354	73	14	to	to	ADP
bionys-354	73	15	imagej	imagej	ADJ
bionys-354	73	16	software	software	NOUN
bionys-354	73	17	analysis	analysis	NOUN
bionys-354	73	18	(	(	PUNCT
bionys-354	73	19	national	national	PROPN
bionys-354	73	20	institute	institute	PROPN
bionys-354	73	21	of	of	ADP
bionys-354	73	22	health	health	PROPN
bionys-354	73	23	,	,	PUNCT
bionys-354	73	24	bethesda	bethesda	PROPN
bionys-354	73	25	,	,	PUNCT
bionys-354	73	26	md	md	PROPN
bionys-354	73	27	,	,	PUNCT
bionys-354	73	28	usa	usa	PROPN
bionys-354	73	29	)	)	PUNCT
bionys-354	73	30	with	with	ADP
bionys-354	73	31	the	the	DET
bionys-354	73	32	aim	aim	NOUN
bionys-354	73	33	of	of	ADP
bionys-354	73	34	measuring	measure	VERB
bionys-354	73	35	the	the	DET
bionys-354	73	36	fluorescence	fluorescence	ADJ
bionys-354	73	37	intensity	intensity	NOUN
bionys-354	73	38	of	of	ADP
bionys-354	73	39	methylated	methylate	VERB
bionys-354	73	40	(	(	PUNCT
bionys-354	73	41	m	m	NOUN
bionys-354	73	42	)	)	PUNCT
bionys-354	73	43	and	and	CCONJ
bionys-354	73	44	unmethylated	unmethylated	ADJ
bionys-354	73	45	(	(	PUNCT
bionys-354	73	46	u	u	NOUN
bionys-354	73	47	)	)	PUNCT
bionys-354	73	48	msp	msp	PROPN
bionys-354	73	49	bands	band	NOUN
bionys-354	73	50	.	.	PUNCT
bionys-354	74	1	m	m	PROPN
bionys-354	74	2	/	/	SYM
bionys-354	74	3	u	u	PROPN
bionys-354	74	4	intensity	intensity	NOUN
bionys-354	74	5	ratio	ratio	NOUN
bionys-354	74	6	values	value	NOUN
bionys-354	74	7	represent	represent	VERB
bionys-354	74	8	the	the	DET
bionys-354	74	9	common	common	ADJ
bionys-354	74	10	approach	approach	NOUN
bionys-354	74	11	of	of	ADP
bionys-354	74	12	semi	semi	ADJ
bionys-354	74	13	-	-	ADJ
bionys-354	74	14	quantitative	quantitative	ADJ
bionys-354	74	15	evaluation	evaluation	NOUN
bionys-354	74	16	of	of	ADP
bionys-354	74	17	the	the	DET
bionys-354	74	18	mgmt	mgmt	PROPN
bionys-354	74	19	promoter	promoter	NOUN
bionys-354	74	20	methylation	methylation	NOUN
bionys-354	74	21	level	level	NOUN
bionys-354	74	22	(	(	PUNCT
bionys-354	74	23	christians	christians	PROPN
bionys-354	74	24	et	et	PROPN
bionys-354	74	25	al	al	PROPN
bionys-354	74	26	.	.	PROPN
bionys-354	74	27	,	,	PUNCT
bionys-354	74	28	2012	2012	NUM
bionys-354	74	29	)	)	PUNCT
bionys-354	74	30	.	.	PUNCT
bionys-354	75	1	results	result	VERB
bionys-354	75	2	ff	ff	NOUN
bionys-354	75	3	and	and	CCONJ
bionys-354	75	4	ffpe	ffpe	PROPN
bionys-354	75	5	dna	dna	PROPN
bionys-354	75	6	isolates	isolate	VERB
bionys-354	75	7	concentrations	concentration	NOUN
bionys-354	75	8	and	and	CCONJ
bionys-354	75	9	purity	purity	NOUN
bionys-354	75	10	of	of	ADP
bionys-354	75	11	genomic	genomic	ADJ
bionys-354	75	12	dna	dna	PROPN
bionys-354	75	13	isolates	isolate	NOUN
bionys-354	75	14	and	and	CCONJ
bionys-354	75	15	bisulfite	bisulfite	NOUN
bionys-354	75	16	-	-	PUNCT
bionys-354	75	17	converted	convert	VERB
bionys-354	75	18	dna	dna	NOUN
bionys-354	75	19	samples	sample	NOUN
bionys-354	75	20	are	be	AUX
bionys-354	75	21	presented	present	VERB
bionys-354	75	22	in	in	ADP
bionys-354	75	23	tab	tab	NOUN
bionys-354	75	24	.	.	PUNCT
bionys-354	76	1	2	2	X
bionys-354	76	2	.	.	X
bionys-354	76	3	gene	gene	NOUN
bionys-354	76	4	primer	primer	NOUN
bionys-354	76	5	sequence	sequence	NOUN
bionys-354	76	6	(	(	PUNCT
bionys-354	76	7	5’-3	5’-3	PROPN
bionys-354	76	8	’	'	PUNCT
bionys-354	76	9	)	)	PUNCT
bionys-354	76	10	amplicon	amplicon	NOUN
bionys-354	76	11	size	size	NOUN
bionys-354	76	12	(	(	PUNCT
bionys-354	76	13	bp	bp	PROPN
bionys-354	76	14	)	)	PUNCT
bionys-354	76	15	mgmt	mgmt	PROPN
bionys-354	76	16	unmethylated	unmethylate	VERB
bionys-354	76	17	(	(	PUNCT
bionys-354	76	18	u	u	NOUN
bionys-354	76	19	)	)	PUNCT
bionys-354	76	20	f	f	NOUN
bionys-354	76	21	:	:	PUNCT
bionys-354	76	22	tttgtgttttgatgtttgtaggtttttgt	tttgtgttttgatgtttgtaggtttttgt	PROPN
bionys-354	76	23	r	r	NOUN
bionys-354	76	24	:	:	PUNCT
bionys-354	76	25	aactccacactcttccaaaaacaaaaca	aactccacactcttccaaaaacaaaaca	NOUN
bionys-354	76	26	93	93	NUM
bionys-354	76	27	mgmt	mgmt	NOUN
bionys-354	76	28	methylated	methylate	VERB
bionys-354	76	29	(	(	PUNCT
bionys-354	76	30	m	m	NOUN
bionys-354	76	31	)	)	PUNCT
bionys-354	77	1	f	f	NOUN
bionys-354	77	2	:	:	PUNCT
bionys-354	77	3	tttcgacgttcgtaggttttcgc	tttcgacgttcgtaggttttcgc	PROPN
bionys-354	77	4	r	r	NOUN
bionys-354	77	5	:	:	PUNCT
bionys-354	77	6	gcactcttccgaaaacgaaacg	gcactcttccgaaaacgaaacg	NOUN
bionys-354	77	7	81	81	NUM
bionys-354	77	8	table	table	NOUN
bionys-354	77	9	1	1	NUM
bionys-354	77	10	.	.	PUNCT
bionys-354	78	1	primer	primer	NOUN
bionys-354	78	2	sequences	sequence	NOUN
bionys-354	78	3	and	and	CCONJ
bionys-354	78	4	amplicon	amplicon	NOUN
bionys-354	78	5	size	size	NOUN
bionys-354	78	6	total	total	ADJ
bionys-354	78	7	yield	yield	NOUN
bionys-354	78	8	of	of	ADP
bionys-354	78	9	dna	dna	PROPN
bionys-354	78	10	(	(	PUNCT
bionys-354	78	11	ng	ng	NOUN
bionys-354	78	12	)	)	PUNCT
bionys-354	78	13	od	od	PROPN
bionys-354	78	14	260/280	260/280	NUM
bionys-354	78	15	yield	yield	NOUN
bionys-354	78	16	of	of	ADP
bionys-354	78	17	bisulfite	bisulfite	NOUN
bionys-354	78	18	-	-	PUNCT
bionys-354	78	19	converted	convert	VERB
bionys-354	78	20	dna	dna	NOUN
bionys-354	78	21	per	per	ADP
bionys-354	78	22	single	single	ADJ
bionys-354	78	23	conversion	conversion	NOUN
bionys-354	78	24	(	(	PUNCT
bionys-354	78	25	ng	ng	NOUN
bionys-354	78	26	)	)	PUNCT
bionys-354	78	27	ffpe	ffpe	NOUN
bionys-354	78	28	dna	dna	PROPN
bionys-354	78	29	isolate	isolate	NOUN
bionys-354	78	30	(	(	PUNCT
bionys-354	78	31	from	from	ADP
bionys-354	78	32	8	8	NUM
bionys-354	78	33	sections	section	NOUN
bionys-354	78	34	with	with	ADP
bionys-354	78	35	a	a	DET
bionys-354	78	36	thickness	thickness	NOUN
bionys-354	78	37	of	of	ADP
bionys-354	78	38	10	10	NUM
bionys-354	78	39	µm	µm	NOUN
bionys-354	78	40	)	)	PUNCT
bionys-354	78	41	4,537.5	4,537.5	NUM
bionys-354	78	42	1.94	1.94	NUM
bionys-354	78	43	2,265.8	2,265.8	NUM
bionys-354	78	44	ff	ff	PROPN
bionys-354	78	45	dna	dna	PROPN
bionys-354	78	46	isolate	isolate	NOUN
bionys-354	78	47	(	(	PUNCT
bionys-354	78	48	50	50	NUM
bionys-354	78	49	mg	mg	NOUN
bionys-354	78	50	of	of	ADP
bionys-354	78	51	tissue	tissue	NOUN
bionys-354	78	52	)	)	PUNCT
bionys-354	78	53	35,209.2	35,209.2	NOUN
bionys-354	78	54	1.87	1.87	NUM
bionys-354	78	55	2,094.4	2,094.4	NUM
bionys-354	78	56	table	table	NOUN
bionys-354	78	57	2	2	NUM
bionys-354	78	58	.	.	PUNCT
bionys-354	78	59	quantity	quantity	NOUN
bionys-354	78	60	and	and	CCONJ
bionys-354	78	61	quality	quality	NOUN
bionys-354	78	62	of	of	ADP
bionys-354	78	63	isolated	isolated	ADJ
bionys-354	78	64	dna	dna	NOUN
bionys-354	78	65	and	and	CCONJ
bionys-354	78	66	bisulfite	bisulfite	NOUN
bionys-354	78	67	converted	convert	VERB
bionys-354	78	68	samples	sample	NOUN
bionys-354	78	69	(	(	PUNCT
bionys-354	78	70	biospec	biospec	NOUN
bionys-354	78	71	–	–	PUNCT
bionys-354	78	72	nano	nano	ADJ
bionys-354	78	73	uv	uv	NOUN
bionys-354	78	74	–	–	PUNCT
bionys-354	78	75	vis	vis	X
bionys-354	78	76	spectrophotometer	spectrophotometer	NOUN
bionys-354	78	77	)	)	PUNCT
bionys-354	78	78	142	142	NUM
bionys-354	78	79	total	total	ADJ
bionys-354	78	80	yield	yield	NOUN
bionys-354	78	81	of	of	ADP
bionys-354	78	82	isolated	isolated	ADJ
bionys-354	78	83	dna	dna	NOUN
bionys-354	78	84	from	from	ADP
bionys-354	78	85	ffpe	ffpe	NOUN
bionys-354	78	86	sample	sample	NOUN
bionys-354	78	87	was	be	AUX
bionys-354	78	88	4,537.5	4,537.5	NUM
bionys-354	78	89	ng	ng	PROPN
bionys-354	78	90	and	and	CCONJ
bionys-354	78	91	35,209.2	35,209.2	NUM
bionys-354	78	92	ng	ng	PROPN
bionys-354	78	93	from	from	ADP
bionys-354	78	94	ff	ff	PROPN
bionys-354	78	95	sample	sample	NOUN
bionys-354	78	96	,	,	PUNCT
bionys-354	78	97	with	with	ADP
bionys-354	78	98	od	od	PROPN
bionys-354	78	99	260/280	260/280	NUM
bionys-354	78	100	values	value	NOUN
bionys-354	78	101	of	of	ADP
bionys-354	78	102	1.94	1.94	NUM
bionys-354	78	103	and	and	CCONJ
bionys-354	78	104	1.87	1.87	NUM
bionys-354	78	105	,	,	PUNCT
bionys-354	78	106	respectively	respectively	ADV
bionys-354	78	107	.	.	PUNCT
bionys-354	79	1	recorded	record	VERB
bionys-354	79	2	yield	yield	NOUN
bionys-354	79	3	of	of	ADP
bionys-354	79	4	bisulfite	bisulfite	NOUN
bionys-354	79	5	-	-	PUNCT
bionys-354	79	6	converted	convert	VERB
bionys-354	79	7	dna	dna	NOUN
bionys-354	79	8	per	per	ADP
bionys-354	79	9	single	single	ADJ
bionys-354	79	10	conversion	conversion	NOUN
bionys-354	79	11	was	be	AUX
bionys-354	79	12	2,265.8	2,265.8	NUM
bionys-354	79	13	ng	ng	PROPN
bionys-354	79	14	for	for	ADP
bionys-354	79	15	ffpe	ffpe	NOUN
bionys-354	79	16	and	and	CCONJ
bionys-354	79	17	2,094.4	2,094.4	NUM
bionys-354	79	18	ng	ng	PROPN
bionys-354	79	19	for	for	ADP
bionys-354	79	20	ff	ff	PROPN
bionys-354	79	21	dna	dna	PROPN
bionys-354	79	22	sample	sample	NOUN
bionys-354	79	23	.	.	PUNCT
bionys-354	80	1	agarose	agarose	NOUN
bionys-354	80	2	gel	gel	NOUN
bionys-354	80	3	images	image	NOUN
bionys-354	80	4	representing	represent	VERB
bionys-354	80	5	the	the	DET
bionys-354	80	6	integrity	integrity	NOUN
bionys-354	80	7	of	of	ADP
bionys-354	80	8	isolated	isolated	ADJ
bionys-354	80	9	dna	dna	NOUN
bionys-354	80	10	are	be	AUX
bionys-354	80	11	shown	show	VERB
bionys-354	80	12	in	in	ADP
bionys-354	80	13	fig	fig	NOUN
bionys-354	80	14	.	.	PUNCT
bionys-354	81	1	1	1	X
bionys-354	81	2	.	.	X
bionys-354	81	3	agarose	agarose	NOUN
bionys-354	81	4	gel	gel	NOUN
bionys-354	81	5	images	image	NOUN
bionys-354	81	6	of	of	ADP
bionys-354	81	7	ff	ff	PROPN
bionys-354	81	8	dna	dna	PROPN
bionys-354	81	9	samples	sample	NOUN
bionys-354	81	10	displayed	display	VERB
bionys-354	81	11	that	that	SCONJ
bionys-354	81	12	most	most	ADJ
bionys-354	81	13	of	of	ADP
bionys-354	81	14	the	the	DET
bionys-354	81	15	dna	dna	PROPN
bionys-354	81	16	fragments	fragment	NOUN
bionys-354	81	17	have	have	AUX
bionys-354	81	18	migrated	migrate	VERB
bionys-354	81	19	conjointly	conjointly	ADV
bionys-354	81	20	with	with	ADP
bionys-354	81	21	the	the	DET
bionys-354	81	22	largest	large	ADJ
bionys-354	81	23	fragments	fragment	NOUN
bionys-354	81	24	of	of	ADP
bionys-354	81	25	the	the	DET
bionys-354	81	26	dna	dna	PROPN
bionys-354	81	27	ladder	ladder	NOUN
bionys-354	81	28	marker	marker	NOUN
bionys-354	81	29	(	(	PUNCT
bionys-354	81	30	~9000	~9000	PROPN
bionys-354	81	31	bp	bp	X
bionys-354	81	32	)	)	PUNCT
bionys-354	81	33	thereby	thereby	ADV
bionys-354	81	34	forming	form	VERB
bionys-354	81	35	a	a	DET
bionys-354	81	36	noticeable	noticeable	ADJ
bionys-354	81	37	band	band	NOUN
bionys-354	81	38	.	.	PUNCT
bionys-354	82	1	in	in	ADP
bionys-354	82	2	contrast	contrast	NOUN
bionys-354	82	3	,	,	PUNCT
bionys-354	82	4	highly	highly	ADV
bionys-354	82	5	degraded	degraded	ADJ
bionys-354	82	6	ffpe	ffpe	NOUN
bionys-354	82	7	dna	dna	NOUN
bionys-354	82	8	samples	sample	NOUN
bionys-354	82	9	were	be	AUX
bionys-354	82	10	presented	present	VERB
bionys-354	82	11	on	on	ADP
bionys-354	82	12	agarose	agarose	NOUN
bionys-354	82	13	gels	gel	NOUN
bionys-354	82	14	as	as	ADP
bionys-354	82	15	characteristic	characteristic	ADJ
bionys-354	82	16	large	large	ADJ
bionys-354	82	17	smears	smear	NOUN
bionys-354	82	18	originating	originate	VERB
bionys-354	82	19	from	from	ADP
bionys-354	82	20	dna	dna	PROPN
bionys-354	82	21	fragments	fragment	NOUN
bionys-354	82	22	of	of	ADP
bionys-354	82	23	various	various	ADJ
bionys-354	82	24	sizes	size	NOUN
bionys-354	82	25	,	,	PUNCT
bionys-354	82	26	mostly	mostly	ADV
bionys-354	82	27	shorter	short	ADJ
bionys-354	82	28	than	than	ADP
bionys-354	82	29	1,264	1,264	NUM
bionys-354	82	30	bp	bp	NOUN
bionys-354	82	31	.	.	PUNCT
bionys-354	83	1	mgmt	mgmt	PROPN
bionys-354	83	2	msp	msp	PROPN
bionys-354	83	3	template	template	NOUN
bionys-354	83	4	dna	dna	PROPN
bionys-354	83	5	concentration	concentration	NOUN
bionys-354	83	6	optimization	optimization	NOUN
bionys-354	83	7	in	in	ADP
bionys-354	83	8	ffpe	ffpe	ADJ
bionys-354	83	9	samples	sample	NOUN
bionys-354	83	10	agarose	agarose	NOUN
bionys-354	83	11	gel	gel	NOUN
bionys-354	83	12	image	image	NOUN
bionys-354	83	13	of	of	ADP
bionys-354	83	14	msp	msp	PROPN
bionys-354	83	15	optimization	optimization	NOUN
bionys-354	83	16	of	of	ADP
bionys-354	83	17	optimal	optimal	ADJ
bionys-354	83	18	template	template	NOUN
bionys-354	83	19	dna	dna	NOUN
bionys-354	83	20	quantity	quantity	NOUN
bionys-354	83	21	is	be	AUX
bionys-354	83	22	presented	present	VERB
bionys-354	83	23	in	in	ADP
bionys-354	83	24	fig	fig	NOUN
bionys-354	83	25	.	.	PUNCT
bionys-354	84	1	2	2	X
bionys-354	84	2	.	.	X
bionys-354	84	3	with	with	ADP
bionys-354	84	4	the	the	DET
bionys-354	84	5	clear	clear	ADJ
bionys-354	84	6	difference	difference	NOUN
bionys-354	84	7	in	in	ADP
bionys-354	84	8	fluorescence	fluorescence	ADJ
bionys-354	84	9	intensity	intensity	NOUN
bionys-354	84	10	of	of	ADP
bionys-354	84	11	both	both	CCONJ
bionys-354	84	12	methylated	methylate	VERB
bionys-354	84	13	and	and	CCONJ
bionys-354	84	14	unmethylated	unmethylated	ADJ
bionys-354	84	15	mgmt	mgmt	PROPN
bionys-354	84	16	msp	msp	PROPN
bionys-354	84	17	products	product	NOUN
bionys-354	84	18	for	for	ADP
bionys-354	84	19	1u	1u	NUM
bionys-354	84	20	and	and	CCONJ
bionys-354	84	21	4u	4u	NOUN
bionys-354	84	22	of	of	ADP
bionys-354	84	23	hotstartaq	hotstartaq	PROPN
bionys-354	84	24	polymerase	polymerase	NOUN
bionys-354	84	25	,	,	PUNCT
bionys-354	84	26	the	the	DET
bionys-354	84	27	most	most	ADV
bionys-354	84	28	appropriate	appropriate	ADJ
bionys-354	84	29	m	m	NOUN
bionys-354	84	30	and	and	CCONJ
bionys-354	84	31	u	u	PROPN
bionys-354	84	32	msp	msp	PROPN
bionys-354	84	33	products	product	NOUN
bionys-354	84	34	were	be	AUX
bionys-354	84	35	recorded	record	VERB
bionys-354	84	36	for	for	ADP
bionys-354	84	37	125	125	NUM
bionys-354	84	38	ng	ng	PROPN
bionys-354	84	39	quantity	quantity	NOUN
bionys-354	84	40	of	of	ADP
bionys-354	84	41	template	template	NOUN
bionys-354	84	42	dna	dna	NOUN
bionys-354	84	43	and	and	CCONJ
bionys-354	84	44	4u	4u	NOUN
bionys-354	84	45	of	of	ADP
bionys-354	84	46	hotstartaq	hotstartaq	PROPN
bionys-354	84	47	polymerase	polymerase	NOUN
bionys-354	84	48	.	.	PUNCT
bionys-354	85	1	biologica	biologica	PROPN
bionys-354	85	2	nyssana	nyssana	PROPN
bionys-354	86	1	●	●	PUNCT
bionys-354	86	2	11	11	NUM
bionys-354	86	3	(	(	PUNCT
bionys-354	86	4	2	2	NUM
bionys-354	86	5	)	)	PUNCT
bionys-354	86	6	december	december	PROPN
bionys-354	86	7	2020	2020	NUM
bionys-354	86	8	:	:	PUNCT
bionys-354	86	9	199	199	NUM
bionys-354	86	10	-	-	SYM
bionys-354	86	11	147	147	NUM
bionys-354	86	12	jovanović	jovanović	NOUN
bionys-354	86	13	et	et	PROPN
bionys-354	86	14	al	al	PROPN
bionys-354	86	15	.	.	PUNCT
bionys-354	87	1	●	●	PUNCT
bionys-354	87	2	optimizing	optimize	VERB
bionys-354	87	3	conditions	condition	NOUN
bionys-354	87	4	for	for	ADP
bionys-354	87	5	mgmt	mgmt	NOUN
bionys-354	87	6	promoter	promoter	NOUN
bionys-354	87	7	methylation	methylation	NOUN
bionys-354	87	8	status	status	NOUN
bionys-354	87	9	analysis	analysis	NOUN
bionys-354	87	10	in	in	ADP
bionys-354	87	11	glioblastoma	glioblastoma	NOUN
bionys-354	87	12	ffpe	ffpe	NOUN
bionys-354	87	13	samples	sample	NOUN
bionys-354	87	14	fig	fig	NOUN
bionys-354	87	15	.	.	PUNCT
bionys-354	88	1	1	1	X
bionys-354	88	2	.	.	X
bionys-354	88	3	agarose	agarose	NOUN
bionys-354	88	4	gel	gel	NOUN
bionys-354	88	5	electrophoresis	electrophoresis	NOUN
bionys-354	88	6	images	image	NOUN
bionys-354	88	7	of	of	ADP
bionys-354	88	8	isolated	isolated	ADJ
bionys-354	88	9	dna	dna	NOUN
bionys-354	88	10	samples	sample	NOUN
bionys-354	88	11	(	(	PUNCT
bionys-354	88	12	~400ng	~400ng	X
bionys-354	88	13	)	)	PUNCT
bionys-354	88	14	a	a	X
bionys-354	88	15	)	)	PUNCT
bionys-354	88	16	high	high	ADJ
bionys-354	88	17	-	-	PUNCT
bionys-354	88	18	quality	quality	NOUN
bionys-354	88	19	dna	dna	PROPN
bionys-354	88	20	isolates	isolate	NOUN
bionys-354	88	21	obtained	obtain	VERB
bionys-354	88	22	from	from	ADP
bionys-354	88	23	ff	ff	NOUN
bionys-354	88	24	samples	sample	NOUN
bionys-354	88	25	(	(	PUNCT
bionys-354	88	26	4000	4000	NUM
bionys-354	88	27	-	-	SYM
bionys-354	88	28	9000	9000	NUM
bionys-354	88	29	bp	bp	PROPN
bionys-354	88	30	fragments	fragment	NOUN
bionys-354	88	31	)	)	PUNCT
bionys-354	88	32	.	.	PUNCT
bionys-354	89	1	lane	lane	NOUN
bionys-354	89	2	1	1	NUM
bionys-354	89	3	–	–	PUNCT
bionys-354	89	4	marker	marker	NOUN
bionys-354	89	5	λ	λ	PROPN
bionys-354	89	6	dna	dna	PROPN
bionys-354	89	7	-	-	PUNCT
bionys-354	89	8	bst	bst	PROPN
bionys-354	89	9	eii	eii	PROPN
bionys-354	89	10	(	(	PUNCT
bionys-354	89	11	new	new	PROPN
bionys-354	89	12	england	england	PROPN
bionys-354	89	13	biolabs	biolabs	PROPN
bionys-354	89	14	®	®	PROPN
bionys-354	89	15	inc	inc	PROPN
bionys-354	89	16	.	.	PROPN
bionys-354	89	17	#	#	SYM
bionys-354	89	18	n3014s	n3014s	PROPN
bionys-354	89	19	)	)	PUNCT
bionys-354	89	20	,	,	PUNCT
bionys-354	89	21	lane	lane	PROPN
bionys-354	89	22	2ff	2ff	ADJ
bionys-354	89	23	dna	dna	PROPN
bionys-354	89	24	sample	sample	NOUN
bionys-354	89	25	(	(	PUNCT
bionys-354	89	26	first	first	ADJ
bionys-354	89	27	dna	dna	PROPN
bionys-354	89	28	elution	elution	NOUN
bionys-354	89	29	)	)	PUNCT
bionys-354	89	30	,	,	PUNCT
bionys-354	89	31	lane	lane	NOUN
bionys-354	89	32	3ff	3ff	ADJ
bionys-354	89	33	dna	dna	PROPN
bionys-354	89	34	sample	sample	NOUN
bionys-354	89	35	(	(	PUNCT
bionys-354	89	36	second	second	ADJ
bionys-354	89	37	dna	dna	PROPN
bionys-354	89	38	elution	elution	NOUN
bionys-354	89	39	)	)	PUNCT
bionys-354	89	40	b	b	X
bionys-354	89	41	)	)	PUNCT
bionys-354	89	42	low	low	ADJ
bionys-354	89	43	-	-	PUNCT
bionys-354	89	44	quality	quality	NOUN
bionys-354	89	45	ffpe	ffpe	NOUN
bionys-354	89	46	dna	dna	NOUN
bionys-354	89	47	samples	sample	NOUN
bionys-354	89	48	with	with	ADP
bionys-354	89	49	highly	highly	ADV
bionys-354	89	50	degraded	degraded	ADJ
bionys-354	89	51	dna	dna	NOUN
bionys-354	89	52	fragments	fragment	NOUN
bionys-354	89	53	:	:	PUNCT
bionys-354	89	54	lane	lane	NOUN
bionys-354	89	55	1	1	NUM
bionys-354	89	56	–	–	PUNCT
bionys-354	89	57	marker	marker	NOUN
bionys-354	89	58	λ	λ	PROPN
bionys-354	89	59	dnabst	dnabst	PROPN
bionys-354	89	60	eii	eii	PROPN
bionys-354	89	61	,	,	PUNCT
bionys-354	89	62	lanes	lane	VERB
bionys-354	89	63	2,3,4	2,3,4	NUM
bionys-354	89	64	ffpe	ffpe	NOUN
bionys-354	89	65	dna	dna	PROPN
bionys-354	89	66	samples	sample	NOUN
bionys-354	89	67	.	.	PUNCT
bionys-354	90	1	fig	fig	NOUN
bionys-354	90	2	.	.	PUNCT
bionys-354	91	1	2	2	X
bionys-354	91	2	.	.	X
bionys-354	91	3	msp	msp	PROPN
bionys-354	91	4	optimization	optimization	NOUN
bionys-354	91	5	for	for	ADP
bionys-354	91	6	ffpe	ffpe	PROPN
bionys-354	91	7	dna	dna	PROPN
bionys-354	91	8	isolates	isolate	VERB
bionys-354	91	9	a	a	PRON
bionys-354	91	10	)	)	PUNCT
bionys-354	91	11	agarose	agarose	NOUN
bionys-354	91	12	gel	gel	NOUN
bionys-354	91	13	image	image	PROPN
bionys-354	91	14	b	b	PROPN
bionys-354	91	15	)	)	PUNCT
bionys-354	91	16	legend	legend	NOUN
bionys-354	91	17	143	143	NUM
bionys-354	91	18	biologica	biologica	PROPN
bionys-354	91	19	nyssana	nyssana	PROPN
bionys-354	92	1	●	●	PUNCT
bionys-354	92	2	11	11	NUM
bionys-354	92	3	(	(	PUNCT
bionys-354	92	4	2	2	NUM
bionys-354	92	5	)	)	PUNCT
bionys-354	92	6	december	december	PROPN
bionys-354	92	7	2020	2020	NUM
bionys-354	92	8	:	:	PUNCT
bionys-354	92	9	199	199	NUM
bionys-354	92	10	-	-	SYM
bionys-354	92	11	147	147	NUM
bionys-354	92	12	jovanović	jovanović	NOUN
bionys-354	92	13	et	et	PROPN
bionys-354	92	14	al	al	PROPN
bionys-354	92	15	.	.	PUNCT
bionys-354	93	1	●	●	PUNCT
bionys-354	93	2	optimizing	optimize	VERB
bionys-354	93	3	conditions	condition	NOUN
bionys-354	93	4	for	for	ADP
bionys-354	93	5	mgmt	mgmt	NOUN
bionys-354	93	6	promoter	promoter	NOUN
bionys-354	93	7	methylation	methylation	NOUN
bionys-354	93	8	status	status	NOUN
bionys-354	93	9	analysis	analysis	NOUN
bionys-354	93	10	in	in	ADP
bionys-354	93	11	glioblastoma	glioblastoma	NOUN
bionys-354	93	12	ffpe	ffpe	NOUN
bionys-354	93	13	samples	sample	NOUN
bionys-354	93	14	fig	fig	NOUN
bionys-354	93	15	.	.	PUNCT
bionys-354	94	1	3	3	X
bionys-354	94	2	.	.	X
bionys-354	94	3	results	result	NOUN
bionys-354	94	4	of	of	ADP
bionys-354	94	5	msp	msp	PROPN
bionys-354	94	6	optimization	optimization	NOUN
bionys-354	94	7	regarding	regard	VERB
bionys-354	94	8	defining	define	VERB
bionys-354	94	9	the	the	DET
bionys-354	94	10	optimal	optimal	ADJ
bionys-354	94	11	amount	amount	NOUN
bionys-354	94	12	of	of	ADP
bionys-354	94	13	hotstartaq	hotstartaq	PROPN
bionys-354	94	14	polymerase	polymerase	NOUN
bionys-354	94	15	.	.	PUNCT
bionys-354	95	1	a	a	DET
bionys-354	95	2	)	)	PUNCT
bionys-354	95	3	original	original	ADJ
bionys-354	95	4	agarose	agarose	NOUN
bionys-354	95	5	gel	gel	NOUN
bionys-354	95	6	image	image	NOUN
bionys-354	95	7	with	with	ADP
bionys-354	95	8	a	a	DET
bionys-354	95	9	legend	legend	NOUN
bionys-354	95	10	on	on	ADP
bionys-354	95	11	the	the	DET
bionys-354	95	12	right	right	NOUN
bionys-354	95	13	,	,	PUNCT
bionys-354	95	14	presenting	present	VERB
bionys-354	95	15	ffpe	ffpe	NOUN
bionys-354	95	16	unmethylated	unmethylated	ADJ
bionys-354	95	17	mgmt	mgmt	PROPN
bionys-354	95	18	promoter	promoter	NOUN
bionys-354	95	19	msp	msp	PROPN
bionys-354	95	20	products	product	NOUN
bionys-354	95	21	with	with	ADP
bionys-354	95	22	1u	1u	NUM
bionys-354	95	23	,	,	PUNCT
bionys-354	95	24	2u	2u	NOUN
bionys-354	95	25	and	and	CCONJ
bionys-354	95	26	4u	4u	NOUN
bionys-354	95	27	of	of	ADP
bionys-354	95	28	hotstartaq	hotstartaq	PROPN
bionys-354	95	29	polymerase	polymerase	NOUN
bionys-354	95	30	alongside	alongside	ADV
bionys-354	95	31	with	with	ADP
bionys-354	95	32	ff	ff	PROPN
bionys-354	95	33	unmethylated	unmethylated	ADJ
bionys-354	95	34	mgmt	mgmt	PROPN
bionys-354	95	35	msp	msp	PROPN
bionys-354	95	36	with	with	ADP
bionys-354	95	37	1u	1u	NUM
bionys-354	95	38	of	of	ADP
bionys-354	95	39	hotstartaq	hotstartaq	PROPN
bionys-354	95	40	polymerase	polymerase	NOUN
bionys-354	95	41	product	product	NOUN
bionys-354	95	42	as	as	ADP
bionys-354	95	43	a	a	DET
bionys-354	95	44	positive	positive	ADJ
bionys-354	95	45	control	control	NOUN
bionys-354	95	46	.	.	PUNCT
bionys-354	96	1	b	b	X
bionys-354	96	2	)	)	PUNCT
bionys-354	96	3	imagej	imagej	ADJ
bionys-354	96	4	software	software	NOUN
bionys-354	96	5	processed	process	VERB
bionys-354	96	6	image	image	NOUN
bionys-354	96	7	with	with	ADP
bionys-354	96	8	fluorescence	fluorescence	ADJ
bionys-354	96	9	intensity	intensity	NOUN
bionys-354	96	10	analysis	analysis	NOUN
bionys-354	96	11	of	of	ADP
bionys-354	96	12	specific	specific	ADJ
bionys-354	96	13	unmethylated	unmethylated	ADJ
bionys-354	96	14	mgmt	mgmt	PROPN
bionys-354	96	15	msp	msp	PROPN
bionys-354	96	16	product	product	NOUN
bionys-354	96	17	bands	band	NOUN
bionys-354	96	18	,	,	PUNCT
bionys-354	96	19	with	with	ADP
bionys-354	96	20	corresponding	correspond	VERB
bionys-354	96	21	chart	chart	NOUN
bionys-354	96	22	representing	represent	VERB
bionys-354	96	23	their	their	PRON
bionys-354	96	24	relative	relative	ADJ
bionys-354	96	25	values	value	NOUN
bionys-354	96	26	.	.	PUNCT
bionys-354	97	1	c	c	X
bionys-354	97	2	)	)	PUNCT
bionys-354	97	3	imagej	imagej	ADV
bionys-354	97	4	fluorescence	fluorescence	ADJ
bionys-354	97	5	intensity	intensity	NOUN
bionys-354	97	6	analysis	analysis	NOUN
bionys-354	97	7	of	of	ADP
bionys-354	97	8	primer	primer	NOUN
bionys-354	97	9	-	-	PUNCT
bionys-354	97	10	dimer	dimer	NOUN
bionys-354	97	11	presence	presence	NOUN
bionys-354	97	12	in	in	ADP
bionys-354	97	13	msp	msp	PROPN
bionys-354	97	14	reactions	reaction	NOUN
bionys-354	97	15	.	.	PUNCT
bionys-354	98	1	144	144	NUM
bionys-354	98	2	biologica	biologica	PROPN
bionys-354	98	3	nyssana	nyssana	PROPN
bionys-354	98	4	●	●	PUNCT
bionys-354	98	5	11	11	NUM
bionys-354	98	6	(	(	PUNCT
bionys-354	98	7	2	2	NUM
bionys-354	98	8	)	)	PUNCT
bionys-354	98	9	december	december	PROPN
bionys-354	98	10	2020	2020	NUM
bionys-354	98	11	:	:	PUNCT
bionys-354	98	12	199	199	NUM
bionys-354	98	13	-	-	SYM
bionys-354	98	14	147	147	NUM
bionys-354	98	15	jovanović	jovanović	NOUN
bionys-354	98	16	et	et	PROPN
bionys-354	98	17	al	al	PROPN
bionys-354	98	18	.	.	PUNCT
bionys-354	99	1	●	●	PUNCT
bionys-354	99	2	optimizing	optimize	VERB
bionys-354	99	3	conditions	condition	NOUN
bionys-354	99	4	for	for	ADP
bionys-354	99	5	mgmt	mgmt	NOUN
bionys-354	99	6	promoter	promoter	NOUN
bionys-354	99	7	methylation	methylation	NOUN
bionys-354	99	8	status	status	NOUN
bionys-354	99	9	analysis	analysis	NOUN
bionys-354	99	10	in	in	ADP
bionys-354	99	11	glioblastoma	glioblastoma	NOUN
bionys-354	99	12	ffpe	ffpe	NOUN
bionys-354	99	13	samples	sample	NOUN
bionys-354	99	14	mgmt	mgmt	PROPN
bionys-354	99	15	msp	msp	PROPN
bionys-354	99	16	hotstartaq	hotstartaq	PROPN
bionys-354	99	17	polymerase	polymerase	NOUN
bionys-354	99	18	concentration	concentration	NOUN
bionys-354	99	19	optimization	optimization	NOUN
bionys-354	99	20	in	in	ADP
bionys-354	99	21	ffpe	ffpe	ADJ
bionys-354	99	22	samples	sample	NOUN
bionys-354	99	23	regarding	regard	VERB
bionys-354	99	24	further	further	ADJ
bionys-354	99	25	determination	determination	NOUN
bionys-354	99	26	of	of	ADP
bionys-354	99	27	the	the	DET
bionys-354	99	28	optimal	optimal	ADJ
bionys-354	99	29	amount	amount	NOUN
bionys-354	99	30	of	of	ADP
bionys-354	99	31	hot	hot	ADJ
bionys-354	99	32	start	start	NOUN
bionys-354	99	33	taq	taq	NOUN
bionys-354	99	34	polymerase	polymerase	NOUN
bionys-354	99	35	,	,	PUNCT
bionys-354	99	36	agarose	agarose	NOUN
bionys-354	99	37	gel	gel	NOUN
bionys-354	99	38	image	image	NOUN
bionys-354	99	39	was	be	AUX
bionys-354	99	40	acquired	acquire	VERB
bionys-354	99	41	following	follow	VERB
bionys-354	99	42	msp	msp	PROPN
bionys-354	99	43	optimization	optimization	NOUN
bionys-354	99	44	and	and	CCONJ
bionys-354	99	45	processed	process	VERB
bionys-354	99	46	with	with	ADP
bionys-354	99	47	imagej	imagej	ADJ
bionys-354	99	48	software	software	NOUN
bionys-354	99	49	analysis	analysis	NOUN
bionys-354	99	50	(	(	PUNCT
bionys-354	99	51	fig	fig	NOUN
bionys-354	99	52	.	.	PUNCT
bionys-354	100	1	3	3	NUM
bionys-354	100	2	)	)	PUNCT
bionys-354	100	3	.	.	PUNCT
bionys-354	101	1	fluorescence	fluorescence	PROPN
bionys-354	101	2	intensity	intensity	NOUN
bionys-354	101	3	values	value	NOUN
bionys-354	101	4	acquired	acquire	VERB
bionys-354	101	5	using	use	VERB
bionys-354	101	6	the	the	DET
bionys-354	101	7	imagej	imagej	ADJ
bionys-354	101	8	software	software	NOUN
bionys-354	101	9	have	have	AUX
bionys-354	101	10	confirmed	confirm	VERB
bionys-354	101	11	the	the	DET
bionys-354	101	12	4u	4u	NOUN
bionys-354	101	13	of	of	ADP
bionys-354	101	14	hotstartaq	hotstartaq	NOUN
bionys-354	101	15	polymerase	polymerase	NOUN
bionys-354	101	16	as	as	ADP
bionys-354	101	17	an	an	DET
bionys-354	101	18	optimal	optimal	ADJ
bionys-354	101	19	amount	amount	NOUN
bionys-354	101	20	for	for	ADP
bionys-354	101	21	successful	successful	ADJ
bionys-354	101	22	msp	msp	PROPN
bionys-354	101	23	mgmt	mgmt	PROPN
bionys-354	101	24	reaction	reaction	NOUN
bionys-354	101	25	in	in	ADP
bionys-354	101	26	ffpe	ffpe	ADJ
bionys-354	101	27	samples	sample	NOUN
bionys-354	101	28	.	.	PUNCT
bionys-354	102	1	among	among	ADP
bionys-354	102	2	tested	test	VERB
bionys-354	102	3	concentrations	concentration	NOUN
bionys-354	102	4	,	,	PUNCT
bionys-354	102	5	the	the	DET
bionys-354	102	6	highest	high	ADJ
bionys-354	102	7	fluorescence	fluorescence	ADJ
bionys-354	102	8	intensity	intensity	NOUN
bionys-354	102	9	value	value	NOUN
bionys-354	102	10	of	of	ADP
bionys-354	102	11	unmethylated	unmethylated	ADJ
bionys-354	102	12	msp	msp	PROPN
bionys-354	102	13	products	product	NOUN
bionys-354	102	14	was	be	AUX
bionys-354	102	15	recorded	record	VERB
bionys-354	102	16	for	for	ADP
bionys-354	102	17	4u	4u	NOUN
bionys-354	102	18	of	of	ADP
bionys-354	102	19	hotstartaq	hotstartaq	PROPN
bionys-354	102	20	polymerase	polymerase	NOUN
bionys-354	102	21	.	.	PUNCT
bionys-354	103	1	it	it	PRON
bionys-354	103	2	was	be	AUX
bionys-354	103	3	equivalent	equivalent	ADJ
bionys-354	103	4	to	to	ADP
bionys-354	103	5	57,43	57,43	NUM
bionys-354	103	6	%	%	NOUN
bionys-354	103	7	of	of	ADP
bionys-354	103	8	the	the	DET
bionys-354	103	9	recorded	record	VERB
bionys-354	103	10	fluorescence	fluorescence	NOUN
bionys-354	103	11	intensity	intensity	NOUN
bionys-354	103	12	value	value	NOUN
bionys-354	103	13	of	of	ADP
bionys-354	103	14	the	the	DET
bionys-354	103	15	reference	reference	NOUN
bionys-354	103	16	ff	ff	PROPN
bionys-354	103	17	dna	dna	PROPN
bionys-354	103	18	mgmt	mgmt	PROPN
bionys-354	103	19	msp	msp	PROPN
bionys-354	103	20	product	product	NOUN
bionys-354	103	21	.	.	PUNCT
bionys-354	104	1	furthermore	furthermore	ADV
bionys-354	104	2	,	,	PUNCT
bionys-354	104	3	the	the	DET
bionys-354	104	4	fluorescence	fluorescence	NOUN
bionys-354	104	5	intensity	intensity	NOUN
bionys-354	104	6	value	value	NOUN
bionys-354	104	7	of	of	ADP
bionys-354	104	8	primer	primer	NOUN
bionys-354	104	9	-	-	PUNCT
bionys-354	104	10	dimers	dimer	NOUN
bionys-354	104	11	was	be	AUX
bionys-354	104	12	the	the	DET
bionys-354	104	13	lowest	low	ADJ
bionys-354	104	14	for	for	ADP
bionys-354	104	15	the	the	DET
bionys-354	104	16	4u	4u	NOUN
bionys-354	104	17	concentration	concentration	NOUN
bionys-354	104	18	,	,	PUNCT
bionys-354	104	19	being	be	AUX
bionys-354	104	20	even	even	ADV
bionys-354	104	21	lower	low	ADJ
bionys-354	104	22	than	than	ADP
bionys-354	104	23	the	the	DET
bionys-354	104	24	one	one	NOUN
bionys-354	104	25	recorded	record	VERB
bionys-354	104	26	for	for	ADP
bionys-354	104	27	the	the	DET
bionys-354	104	28	reference	reference	NOUN
bionys-354	104	29	ff	ff	PROPN
bionys-354	104	30	dna	dna	PROPN
bionys-354	104	31	template	template	NOUN
bionys-354	104	32	msp	msp	NOUN
bionys-354	104	33	37.16	37.16	NUM
bionys-354	104	34	%	%	NOUN
bionys-354	104	35	of	of	ADP
bionys-354	104	36	the	the	DET
bionys-354	104	37	reference	reference	NOUN
bionys-354	104	38	value	value	NOUN
bionys-354	104	39	.	.	PUNCT
bionys-354	105	1	finally	finally	ADV
bionys-354	105	2	,	,	PUNCT
bionys-354	105	3	only	only	ADV
bionys-354	105	4	for	for	ADP
bionys-354	105	5	the	the	DET
bionys-354	105	6	4u	4u	NOUN
bionys-354	105	7	hotstartaq	hotstartaq	NOUN
bionys-354	105	8	polymerase	polymerase	NOUN
bionys-354	105	9	concentration	concentration	NOUN
bionys-354	105	10	was	be	AUX
bionys-354	105	11	documented	document	VERB
bionys-354	105	12	that	that	SCONJ
bionys-354	105	13	the	the	DET
bionys-354	105	14	mgmt	mgmt	PROPN
bionys-354	105	15	product	product	NOUN
bionys-354	105	16	/	/	SYM
bionys-354	105	17	primer	primer	NOUN
bionys-354	105	18	-	-	PUNCT
bionys-354	105	19	dimer	dimer	NOUN
bionys-354	105	20	fluorescence	fluorescence	NOUN
bionys-354	105	21	intensity	intensity	NOUN
bionys-354	105	22	ratio	ratio	NOUN
bionys-354	105	23	exceeds	exceed	VERB
bionys-354	105	24	100	100	NUM
bionys-354	105	25	%	%	NOUN
bionys-354	105	26	of	of	ADP
bionys-354	105	27	the	the	DET
bionys-354	105	28	reference	reference	NOUN
bionys-354	105	29	value	value	NOUN
bionys-354	105	30	(	(	PUNCT
bionys-354	105	31	113.12	113.12	NUM
bionys-354	105	32	%	%	NOUN
bionys-354	105	33	)	)	PUNCT
bionys-354	105	34	,	,	PUNCT
bionys-354	105	35	suggesting	suggest	VERB
bionys-354	105	36	optimal	optimal	ADJ
bionys-354	105	37	reaction	reaction	NOUN
bionys-354	105	38	conditions	condition	NOUN
bionys-354	105	39	(	(	PUNCT
bionys-354	105	40	tab	tab	NOUN
bionys-354	105	41	.	.	NOUN
bionys-354	105	42	3	3	NUM
bionys-354	105	43	and	and	CCONJ
bionys-354	105	44	fig	fig	NOUN
bionys-354	105	45	.	.	PUNCT
bionys-354	106	1	4	4	NUM
bionys-354	106	2	)	)	PUNCT
bionys-354	106	3	.	.	PUNCT
bionys-354	107	1	mgmt	mgmt	PROPN
bionys-354	107	2	methylation	methylation	PROPN
bionys-354	107	3	assesment	assesment	NOUN
bionys-354	107	4	in	in	ADP
bionys-354	107	5	ffpe	ffpe	NOUN
bionys-354	107	6	and	and	CCONJ
bionys-354	107	7	ff	ff	ADJ
bionys-354	107	8	sample	sample	NOUN
bionys-354	107	9	figure	figure	NOUN
bionys-354	107	10	5	5	NUM
bionys-354	107	11	(	(	PUNCT
bionys-354	107	12	a	a	NOUN
bionys-354	107	13	)	)	PUNCT
bionys-354	107	14	shows	show	VERB
bionys-354	107	15	the	the	DET
bionys-354	107	16	agarose	agarose	NOUN
bionys-354	107	17	gel	gel	NOUN
bionys-354	107	18	image	image	NOUN
bionys-354	107	19	of	of	ADP
bionys-354	107	20	mgmt	mgmt	PROPN
bionys-354	107	21	msp	msp	PROPN
bionys-354	107	22	reactions	reaction	NOUN
bionys-354	107	23	which	which	PRON
bionys-354	107	24	included	include	VERB
bionys-354	107	25	bisulfite	bisulfite	NOUN
bionys-354	107	26	-	-	PUNCT
bionys-354	107	27	converted	convert	VERB
bionys-354	107	28	template	template	NOUN
bionys-354	107	29	dna	dna	NOUN
bionys-354	107	30	of	of	ADP
bionys-354	107	31	the	the	DET
bionys-354	107	32	same	same	ADJ
bionys-354	107	33	patient	patient	NOUN
bionys-354	107	34	originating	originating	NOUN
bionys-354	107	35	from	from	ADP
bionys-354	107	36	both	both	PRON
bionys-354	107	37	ffpe	ffpe	NOUN
bionys-354	107	38	and	and	CCONJ
bionys-354	107	39	ff	ff	NOUN
bionys-354	107	40	samples	sample	NOUN
bionys-354	107	41	,	,	PUNCT
bionys-354	107	42	alongside	alongside	ADP
bionys-354	107	43	with	with	ADP
bionys-354	107	44	randomly	randomly	ADV
bionys-354	107	45	chosen	choose	VERB
bionys-354	107	46	ffpe	ffpe	PROPN
bionys-354	107	47	sample	sample	NOUN
bionys-354	107	48	from	from	ADP
bionys-354	107	49	another	another	DET
bionys-354	107	50	gbm	gbm	NOUN
bionys-354	107	51	patient	patient	NOUN
bionys-354	107	52	.	.	PUNCT
bionys-354	108	1	m	m	X
bionys-354	108	2	/	/	SYM
bionys-354	108	3	u	u	NOUN
bionys-354	108	4	ratio	ratio	NOUN
bionys-354	108	5	values	value	NOUN
bionys-354	108	6	of	of	ADP
bionys-354	108	7	ff	ff	NOUN
bionys-354	108	8	and	and	CCONJ
bionys-354	108	9	ffpe	ffpe	NOUN
bionys-354	108	10	samples	sample	NOUN
bionys-354	108	11	were	be	AUX
bionys-354	108	12	acquired	acquire	VERB
bionys-354	108	13	via	via	ADP
bionys-354	108	14	imagej	imagej	ADJ
bionys-354	108	15	software	software	NOUN
bionys-354	108	16	analysis	analysis	NOUN
bionys-354	108	17	fig	fig	NOUN
bionys-354	108	18	.	.	PUNCT
bionys-354	109	1	5	5	NUM
bionys-354	109	2	(	(	PUNCT
bionys-354	109	3	b	b	NOUN
bionys-354	109	4	)	)	PUNCT
bionys-354	109	5	.	.	PUNCT
bionys-354	110	1	as	as	ADP
bionys-354	110	2	a	a	DET
bionys-354	110	3	result	result	NOUN
bionys-354	110	4	of	of	ADP
bionys-354	110	5	imagej	imagej	ADJ
bionys-354	110	6	software	software	NOUN
bionys-354	110	7	analysis	analysis	NOUN
bionys-354	110	8	,	,	PUNCT
bionys-354	110	9	both	both	CCONJ
bionys-354	110	10	the	the	DET
bionys-354	110	11	ffpe	ffpe	NOUN
bionys-354	110	12	and	and	CCONJ
bionys-354	110	13	ff	ff	NOUN
bionys-354	110	14	samples	sample	NOUN
bionys-354	110	15	were	be	AUX
bionys-354	110	16	assessed	assess	VERB
bionys-354	110	17	as	as	ADP
bionys-354	110	18	strongly	strongly	ADV
bionys-354	110	19	methylated	methylate	VERB
bionys-354	110	20	semi	semi	ADJ
bionys-354	110	21	-	-	ADJ
bionys-354	110	22	quantitative	quantitative	ADJ
bionys-354	110	23	category	category	NOUN
bionys-354	110	24	of	of	ADP
bionys-354	110	25	mgmt	mgmt	PROPN
bionys-354	110	26	promoter	promoter	NOUN
bionys-354	110	27	methylation	methylation	NOUN
bionys-354	110	28	(	(	PUNCT
bionys-354	110	29	m	m	NOUN
bionys-354	110	30	/	/	SYM
bionys-354	110	31	u	u	NOUN
bionys-354	110	32	ratio	ratio	NOUN
bionys-354	110	33	<	<	X
bionys-354	110	34	1	1	NUM
bionys-354	110	35	)	)	PUNCT
bionys-354	110	36	.	.	PUNCT
bionys-354	111	1	the	the	DET
bionys-354	111	2	observed	observe	VERB
bionys-354	111	3	difference	difference	NOUN
bionys-354	111	4	in	in	ADP
bionys-354	111	5	m	m	PROPN
bionys-354	111	6	/	/	SYM
bionys-354	111	7	u	u	NOUN
bionys-354	111	8	ratio	ratio	NOUN
bionys-354	111	9	values	value	NOUN
bionys-354	111	10	was	be	AUX
bionys-354	111	11	8.47	8.47	NUM
bionys-354	111	12	%	%	NOUN
bionys-354	111	13	(	(	PUNCT
bionys-354	111	14	tab	tab	NOUN
bionys-354	111	15	.	.	NOUN
bionys-354	111	16	4	4	NUM
bionys-354	111	17	)	)	PUNCT
bionys-354	111	18	.	.	PUNCT
bionys-354	112	1	discussion	discussion	NOUN
bionys-354	112	2	results	result	VERB
bionys-354	112	3	from	from	ADP
bionys-354	112	4	our	our	PRON
bionys-354	112	5	study	study	NOUN
bionys-354	112	6	have	have	AUX
bionys-354	112	7	confirmed	confirm	VERB
bionys-354	112	8	the	the	DET
bionys-354	112	9	great	great	ADJ
bionys-354	112	10	difference	difference	NOUN
bionys-354	112	11	in	in	ADP
bionys-354	112	12	dna	dna	PROPN
bionys-354	112	13	fragmentation	fragmentation	NOUN
bionys-354	112	14	level	level	NOUN
bionys-354	112	15	between	between	ADP
bionys-354	112	16	ff	ff	NOUN
bionys-354	112	17	and	and	CCONJ
bionys-354	112	18	ffpe	ffpe	ADJ
bionys-354	112	19	dna	dna	PROPN
bionys-354	112	20	isolates	isolate	NOUN
bionys-354	112	21	(	(	PUNCT
bionys-354	112	22	fig	fig	NOUN
bionys-354	112	23	.	.	PUNCT
bionys-354	113	1	1	1	NUM
bionys-354	113	2	)	)	PUNCT
bionys-354	113	3	.	.	PUNCT
bionys-354	114	1	in	in	ADP
bionys-354	114	2	contrast	contrast	NOUN
bionys-354	114	3	with	with	ADP
bionys-354	114	4	the	the	DET
bionys-354	114	5	ff	ff	NOUN
bionys-354	114	6	sample	sample	NOUN
bionys-354	114	7	,	,	PUNCT
bionys-354	114	8	the	the	DET
bionys-354	114	9	ffpe	ffpe	NOUN
bionys-354	114	10	sample	sample	NOUN
bionys-354	114	11	from	from	ADP
bionys-354	114	12	our	our	PRON
bionys-354	114	13	study	study	NOUN
bionys-354	114	14	was	be	AUX
bionys-354	114	15	in	in	ADP
bionys-354	114	16	great	great	ADJ
bionys-354	114	17	portion	portion	NOUN
bionys-354	114	18	consisted	consist	VERB
bionys-354	114	19	of	of	ADP
bionys-354	114	20	dna	dna	PROPN
bionys-354	114	21	fragments	fragment	NOUN
bionys-354	114	22	shorter	short	ADJ
bionys-354	114	23	than	than	ADP
bionys-354	114	24	~1,000	~1,000	PROPN
bionys-354	114	25	bp	bp	PROPN
bionys-354	114	26	,	,	PUNCT
bionys-354	114	27	which	which	PRON
bionys-354	114	28	were	be	AUX
bionys-354	114	29	forming	form	VERB
bionys-354	114	30	a	a	DET
bionys-354	114	31	smear	smear	NOUN
bionys-354	114	32	and	and	CCONJ
bionys-354	114	33	lacking	lack	VERB
bionys-354	114	34	a	a	DET
bionys-354	114	35	distinguishable	distinguishable	ADJ
bionys-354	114	36	band	band	NOUN
bionys-354	114	37	on	on	ADP
bionys-354	114	38	the	the	DET
bionys-354	114	39	agarose	agarose	NOUN
bionys-354	114	40	gel	gel	NOUN
bionys-354	114	41	.	.	PUNCT
bionys-354	115	1	this	this	DET
bionys-354	115	2	observation	observation	NOUN
bionys-354	115	3	is	be	AUX
bionys-354	115	4	consistent	consistent	ADJ
bionys-354	115	5	with	with	ADP
bionys-354	115	6	previously	previously	ADV
bionys-354	115	7	described	describe	VERB
bionys-354	115	8	ffpe	ffpe	NOUN
bionys-354	115	9	dna	dna	PROPN
bionys-354	115	10	isolate	isolate	VERB
bionys-354	115	11	properties	property	NOUN
bionys-354	115	12	originating	originate	VERB
bionys-354	115	13	from	from	ADP
bionys-354	115	14	the	the	DET
bionys-354	115	15	tissue	tissue	NOUN
bionys-354	115	16	preparation	preparation	NOUN
bionys-354	115	17	and	and	CCONJ
bionys-354	115	18	fixation	fixation	NOUN
bionys-354	115	19	process	process	NOUN
bionys-354	115	20	.	.	PUNCT
bionys-354	116	1	namely	namely	ADV
bionys-354	116	2	,	,	PUNCT
bionys-354	116	3	it	it	PRON
bionys-354	116	4	was	be	AUX
bionys-354	116	5	shown	show	VERB
bionys-354	116	6	that	that	SCONJ
bionys-354	116	7	mechanical	mechanical	ADJ
bionys-354	116	8	stress	stress	NOUN
bionys-354	116	9	resulting	result	VERB
bionys-354	116	10	from	from	ADP
bionys-354	116	11	cross	cross	ADJ
bionys-354	116	12	-	-	ADJ
bionys-354	116	13	linking	link	VERB
bionys-354	116	14	during	during	ADP
bionys-354	116	15	the	the	DET
bionys-354	116	16	fixation	fixation	NOUN
bionys-354	116	17	process	process	NOUN
bionys-354	116	18	,	,	PUNCT
bionys-354	116	19	the	the	DET
bionys-354	116	20	concentration	concentration	NOUN
bionys-354	116	21	of	of	ADP
bionys-354	116	22	formalin	formalin	NOUN
bionys-354	116	23	,	,	PUNCT
bionys-354	116	24	ph	ph	VERB
bionys-354	116	25	,	,	PUNCT
bionys-354	116	26	and	and	CCONJ
bionys-354	116	27	salt	salt	NOUN
bionys-354	116	28	,	,	PUNCT
bionys-354	116	29	the	the	DET
bionys-354	116	30	temperature	temperature	NOUN
bionys-354	116	31	,	,	PUNCT
bionys-354	116	32	and	and	CCONJ
bionys-354	116	33	tissue	tissue	NOUN
bionys-354	116	34	type	type	NOUN
bionys-354	116	35	have	have	VERB
bionys-354	116	36	a	a	DET
bionys-354	116	37	great	great	ADJ
bionys-354	116	38	impact	impact	NOUN
bionys-354	116	39	on	on	ADP
bionys-354	116	40	the	the	DET
bionys-354	116	41	quality	quality	NOUN
bionys-354	116	42	of	of	ADP
bionys-354	116	43	the	the	DET
bionys-354	116	44	ffpe	ffpe	NOUN
bionys-354	116	45	-	-	PUNCT
bionys-354	116	46	derived	derive	VERB
bionys-354	116	47	dna	dna	NOUN
bionys-354	116	48	(	(	PUNCT
bionys-354	116	49	ludgate	ludgate	PROPN
bionys-354	116	50	et	et	PROPN
bionys-354	116	51	al	al	PROPN
bionys-354	116	52	.	.	PROPN
bionys-354	116	53	,	,	PUNCT
bionys-354	116	54	2017	2017	NUM
bionys-354	116	55	)	)	PUNCT
bionys-354	116	56	.	.	PUNCT
bionys-354	117	1	among	among	ADP
bionys-354	117	2	such	such	ADJ
bionys-354	117	3	factors	factor	NOUN
bionys-354	117	4	,	,	PUNCT
bionys-354	117	5	the	the	DET
bionys-354	117	6	deparaffinization	deparaffinization	NOUN
bionys-354	117	7	was	be	AUX
bionys-354	117	8	emphasized	emphasize	VERB
bionys-354	117	9	as	as	ADP
bionys-354	117	10	the	the	DET
bionys-354	117	11	crucial	crucial	ADJ
bionys-354	117	12	process	process	NOUN
bionys-354	117	13	which	which	PRON
bionys-354	117	14	reduces	reduce	VERB
bionys-354	117	15	the	the	DET
bionys-354	117	16	quality	quality	NOUN
bionys-354	117	17	of	of	ADP
bionys-354	117	18	the	the	DET
bionys-354	117	19	isolated	isolated	ADJ
bionys-354	117	20	dna	dna	NOUN
bionys-354	117	21	(	(	PUNCT
bionys-354	117	22	sengüven	sengüven	NOUN
bionys-354	117	23	et	et	PROPN
bionys-354	117	24	al	al	PROPN
bionys-354	117	25	.	.	PROPN
bionys-354	117	26	,	,	PUNCT
bionys-354	117	27	total	total	ADJ
bionys-354	117	28	amount	amount	NOUN
bionys-354	117	29	of	of	ADP
bionys-354	117	30	hotstartaq	hotstartaq	PROPN
bionys-354	117	31	polymerase	polymerase	NOUN
bionys-354	117	32	units	unit	NOUN
bionys-354	117	33	in	in	ADP
bionys-354	117	34	msp	msp	PROPN
bionys-354	117	35	reaction	reaction	NOUN
bionys-354	117	36	1u	1u	NUM
bionys-354	117	37	2u	2u	NOUN
bionys-354	117	38	4u	4u	NOUN
bionys-354	117	39	fluorescence	fluorescence	NOUN
bionys-354	117	40	intensity	intensity	NOUN
bionys-354	117	41	of	of	ADP
bionys-354	117	42	unmethylated	unmethylated	ADJ
bionys-354	117	43	mgmt	mgmt	NOUN
bionys-354	117	44	mps	mps	PROPN
bionys-354	117	45	product	product	NOUN
bionys-354	117	46	compared	compare	VERB
bionys-354	117	47	to	to	ADP
bionys-354	117	48	ff	ff	ADJ
bionys-354	117	49	refference	refference	NOUN
bionys-354	117	50	(	(	PUNCT
bionys-354	117	51	%	%	INTJ
bionys-354	117	52	)	)	PUNCT
bionys-354	117	53	48.27	48.27	NUM
bionys-354	117	54	34.67	34.67	NUM
bionys-354	117	55	57.43	57.43	NUM
bionys-354	117	56	fluorescence	fluorescence	NOUN
bionys-354	117	57	intensity	intensity	NOUN
bionys-354	117	58	of	of	ADP
bionys-354	117	59	primer	primer	NOUN
bionys-354	117	60	-	-	PUNCT
bionys-354	117	61	dimers	dimer	NOUN
bionys-354	117	62	compared	compare	VERB
bionys-354	117	63	to	to	ADP
bionys-354	117	64	ff	ff	ADJ
bionys-354	117	65	refference	refference	NOUN
bionys-354	117	66	(	(	PUNCT
bionys-354	117	67	%	%	INTJ
bionys-354	117	68	)	)	PUNCT
bionys-354	117	69	200.60	200.60	NUM
bionys-354	117	70	219.85	219.85	NUM
bionys-354	117	71	37.16	37.16	NUM
bionys-354	117	72	product	product	NOUN
bionys-354	117	73	/	/	SYM
bionys-354	117	74	primer	primer	NOUN
bionys-354	117	75	-	-	PUNCT
bionys-354	117	76	dimer	dimer	NOUN
bionys-354	117	77	intensity	intensity	NOUN
bionys-354	117	78	ratio	ratio	NOUN
bionys-354	117	79	compared	compare	VERB
bionys-354	117	80	to	to	ADP
bionys-354	117	81	ff	ff	ADJ
bionys-354	117	82	refference	refference	NOUN
bionys-354	117	83	(	(	PUNCT
bionys-354	117	84	%	%	INTJ
bionys-354	117	85	)	)	PUNCT
bionys-354	117	86	49.10	49.10	NUM
bionys-354	117	87	36.30	36.30	NUM
bionys-354	117	88	113.12	113.12	NUM
bionys-354	117	89	table	table	NOUN
bionys-354	117	90	3	3	NUM
bionys-354	117	91	.	.	PUNCT
bionys-354	118	1	fluorescence	fluorescence	NOUN
bionys-354	118	2	intensity	intensity	NOUN
bionys-354	118	3	values	value	NOUN
bionys-354	118	4	of	of	ADP
bionys-354	118	5	3	3	NUM
bionys-354	118	6	different	different	ADJ
bionys-354	118	7	concentrations	concentration	NOUN
bionys-354	118	8	of	of	ADP
bionys-354	118	9	hotstartaq	hotstartaq	PROPN
bionys-354	118	10	polymerase	polymerase	NOUN
bionys-354	118	11	obtained	obtain	VERB
bionys-354	118	12	using	use	VERB
bionys-354	118	13	imagej	imagej	ADJ
bionys-354	118	14	software	software	NOUN
bionys-354	118	15	analysis	analysis	NOUN
bionys-354	118	16	fig	fig	NOUN
bionys-354	118	17	.	.	PUNCT
bionys-354	119	1	4	4	X
bionys-354	119	2	.	.	X
bionys-354	119	3	mgmt	mgmt	NOUN
bionys-354	119	4	product	product	NOUN
bionys-354	119	5	/	/	SYM
bionys-354	119	6	primer	primer	NOUN
bionys-354	119	7	-	-	PUNCT
bionys-354	119	8	dimer	dimer	NOUN
bionys-354	119	9	fluorescence	fluorescence	NOUN
bionys-354	119	10	intensity	intensity	NOUN
bionys-354	119	11	ratios	ratio	NOUN
bionys-354	119	12	detected	detect	VERB
bionys-354	119	13	for	for	ADP
bionys-354	119	14	125	125	NUM
bionys-354	119	15	ng	ng	PROPN
bionys-354	119	16	of	of	ADP
bionys-354	119	17	template	template	NOUN
bionys-354	119	18	dna	dna	NOUN
bionys-354	119	19	and	and	CCONJ
bionys-354	119	20	3	3	NUM
bionys-354	119	21	different	different	ADJ
bionys-354	119	22	concentrations	concentration	NOUN
bionys-354	119	23	of	of	ADP
bionys-354	119	24	hotstartaq	hotstartaq	NOUN
bionys-354	119	25	polymerase	polymerase	NOUN
bionys-354	119	26	in	in	ADP
bionys-354	119	27	ffpe	ffpe	NOUN
bionys-354	119	28	and	and	CCONJ
bionys-354	119	29	reference	reference	NOUN
bionys-354	119	30	ff	ff	PROPN
bionys-354	119	31	dna	dna	PROPN
bionys-354	119	32	bisulfiteconverted	bisulfiteconverte	VERB
bionys-354	119	33	samples	sample	NOUN
bionys-354	119	34	145	145	NUM
bionys-354	119	35	2014	2014	NUM
bionys-354	119	36	)	)	PUNCT
bionys-354	119	37	.	.	PUNCT
bionys-354	120	1	given	give	VERB
bionys-354	120	2	the	the	DET
bionys-354	120	3	fact	fact	NOUN
bionys-354	120	4	that	that	SCONJ
bionys-354	120	5	bisulfite	bisulfite	NOUN
bionys-354	120	6	conversion	conversion	NOUN
bionys-354	120	7	further	far	ADV
bionys-354	120	8	degrades	degrade	VERB
bionys-354	120	9	dna	dna	PROPN
bionys-354	120	10	,	,	PUNCT
bionys-354	120	11	the	the	DET
bionys-354	120	12	challenges	challenge	NOUN
bionys-354	120	13	which	which	PRON
bionys-354	120	14	arise	arise	VERB
bionys-354	120	15	in	in	ADP
bionys-354	120	16	dna	dna	PROPN
bionys-354	120	17	methylation	methylation	NOUN
bionys-354	120	18	analysis	analysis	NOUN
bionys-354	120	19	are	be	AUX
bionys-354	120	20	no	no	PRON
bionys-354	120	21	surprising	surprising	ADJ
bionys-354	120	22	(	(	PUNCT
bionys-354	120	23	patterson	patterson	PROPN
bionys-354	120	24	et	et	PROPN
bionys-354	120	25	al	al	PROPN
bionys-354	120	26	.	.	PROPN
bionys-354	120	27	,	,	PUNCT
bionys-354	120	28	2011	2011	NUM
bionys-354	120	29	)	)	PUNCT
bionys-354	120	30	.	.	PUNCT
bionys-354	121	1	as	as	ADP
bionys-354	121	2	a	a	DET
bionys-354	121	3	consequence	consequence	NOUN
bionys-354	121	4	,	,	PUNCT
bionys-354	121	5	msp	msp	PROPN
bionys-354	121	6	reaction	reaction	NOUN
bionys-354	121	7	conditions	condition	NOUN
bionys-354	121	8	concerning	concern	VERB
bionys-354	121	9	methylation	methylation	NOUN
bionys-354	121	10	analysis	analysis	NOUN
bionys-354	121	11	may	may	AUX
bionys-354	121	12	significantly	significantly	ADV
bionys-354	121	13	differ	differ	VERB
bionys-354	121	14	in	in	ADP
bionys-354	121	15	case	case	NOUN
bionys-354	121	16	of	of	ADP
bionys-354	121	17	using	use	VERB
bionys-354	121	18	the	the	DET
bionys-354	121	19	ffpe	ffpe	NOUN
bionys-354	121	20	instead	instead	ADV
bionys-354	121	21	of	of	ADP
bionys-354	121	22	the	the	DET
bionys-354	121	23	ff	ff	NOUN
bionys-354	121	24	bisulfite	bisulfite	NOUN
bionys-354	121	25	-	-	PUNCT
bionys-354	121	26	converted	convert	VERB
bionys-354	121	27	sample	sample	NOUN
bionys-354	121	28	as	as	ADP
bionys-354	121	29	template	template	NOUN
bionys-354	121	30	dna	dna	NOUN
bionys-354	121	31	.	.	PUNCT
bionys-354	122	1	considering	consider	VERB
bionys-354	122	2	the	the	DET
bionys-354	122	3	significance	significance	NOUN
bionys-354	122	4	of	of	ADP
bionys-354	122	5	ffpe	ffpe	ADJ
bionys-354	122	6	tissues	tissue	NOUN
bionys-354	122	7	as	as	ADP
bionys-354	122	8	a	a	DET
bionys-354	122	9	valuable	valuable	ADJ
bionys-354	122	10	dna	dna	PROPN
bionys-354	122	11	source	source	NOUN
bionys-354	122	12	for	for	ADP
bionys-354	122	13	mgmt	mgmt	PROPN
bionys-354	122	14	methylation	methylation	NOUN
bionys-354	122	15	analysis	analysis	NOUN
bionys-354	122	16	in	in	ADP
bionys-354	122	17	gbm	gbm	NOUN
bionys-354	122	18	patients	patient	NOUN
bionys-354	122	19	,	,	PUNCT
bionys-354	122	20	as	as	ADV
bionys-354	122	21	well	well	ADV
bionys-354	122	22	as	as	ADP
bionys-354	122	23	for	for	ADP
bionys-354	122	24	clinical	clinical	ADJ
bionys-354	122	25	and	and	CCONJ
bionys-354	122	26	cancer	cancer	NOUN
bionys-354	122	27	research	research	NOUN
bionys-354	122	28	in	in	ADP
bionys-354	122	29	general	general	ADJ
bionys-354	122	30	,	,	PUNCT
bionys-354	122	31	our	our	PRON
bionys-354	122	32	main	main	ADJ
bionys-354	122	33	goal	goal	NOUN
bionys-354	122	34	was	be	AUX
bionys-354	122	35	to	to	PART
bionys-354	122	36	reveal	reveal	VERB
bionys-354	122	37	the	the	DET
bionys-354	122	38	optimal	optimal	ADJ
bionys-354	122	39	mgmt	mgmt	NOUN
bionys-354	122	40	promoter	promoter	NOUN
bionys-354	122	41	methylation	methylation	NOUN
bionys-354	122	42	msp	msp	PROPN
bionys-354	122	43	reaction	reaction	NOUN
bionys-354	122	44	conditions	condition	NOUN
bionys-354	122	45	which	which	PRON
bionys-354	122	46	could	could	AUX
bionys-354	122	47	bring	bring	VERB
bionys-354	122	48	the	the	DET
bionys-354	122	49	results	result	NOUN
bionys-354	122	50	as	as	ADV
bionys-354	122	51	valid	valid	ADJ
bionys-354	122	52	as	as	ADP
bionys-354	122	53	those	those	PRON
bionys-354	122	54	involving	involve	VERB
bionys-354	122	55	ff	ff	NOUN
bionys-354	122	56	-	-	PUNCT
bionys-354	122	57	derived	derive	VERB
bionys-354	122	58	dna	dna	NOUN
bionys-354	122	59	.	.	PUNCT
bionys-354	123	1	the	the	DET
bionys-354	123	2	most	most	ADV
bionys-354	123	3	significant	significant	ADJ
bionys-354	123	4	msp	msp	PROPN
bionys-354	123	5	reaction	reaction	NOUN
bionys-354	123	6	parameters	parameter	NOUN
bionys-354	123	7	–	–	PUNCT
bionys-354	123	8	taq	taq	NOUN
bionys-354	123	9	polymerase	polymerase	NOUN
bionys-354	123	10	and	and	CCONJ
bionys-354	123	11	template	template	NOUN
bionys-354	123	12	dna	dna	NOUN
bionys-354	123	13	concentrations	concentration	NOUN
bionys-354	123	14	were	be	AUX
bionys-354	123	15	selected	select	VERB
bionys-354	123	16	considering	consider	VERB
bionys-354	123	17	the	the	DET
bionys-354	123	18	findings	finding	NOUN
bionys-354	123	19	of	of	ADP
bionys-354	123	20	improved	improve	VERB
bionys-354	123	21	pcr	pcr	ADJ
bionys-354	123	22	performance	performance	NOUN
bionys-354	123	23	in	in	ADP
bionys-354	123	24	ffpe	ffpe	ADJ
bionys-354	123	25	tissue	tissue	NOUN
bionys-354	123	26	samples	sample	NOUN
bionys-354	123	27	presented	present	VERB
bionys-354	123	28	by	by	ADP
bionys-354	123	29	dietrich	dietrich	PROPN
bionys-354	123	30	et	et	PROPN
bionys-354	123	31	al	al	PROPN
bionys-354	123	32	.	.	PROPN
bionys-354	124	1	(	(	PUNCT
bionys-354	124	2	2013	2013	NUM
bionys-354	124	3	)	)	PUNCT
bionys-354	124	4	.	.	PUNCT
bionys-354	125	1	primarily	primarily	ADV
bionys-354	125	2	,	,	PUNCT
bionys-354	125	3	our	our	PRON
bionys-354	125	4	study	study	NOUN
bionys-354	125	5	tested	test	VERB
bionys-354	125	6	4	4	NUM
bionys-354	125	7	concentrations	concentration	NOUN
bionys-354	125	8	of	of	ADP
bionys-354	125	9	template	template	NOUN
bionys-354	125	10	dna	dna	NOUN
bionys-354	125	11	(	(	PUNCT
bionys-354	125	12	31.25	31.25	NUM
bionys-354	125	13	ng	ng	PROPN
bionys-354	125	14	,	,	PUNCT
bionys-354	125	15	62.5	62.5	NUM
bionys-354	125	16	ng	ng	PROPN
bionys-354	125	17	,	,	PUNCT
bionys-354	125	18	125	125	NUM
bionys-354	125	19	ng	ng	PROPN
bionys-354	125	20	,	,	PUNCT
bionys-354	125	21	and	and	CCONJ
bionys-354	125	22	250	250	NUM
bionys-354	125	23	ng	ng	PROPN
bionys-354	125	24	)	)	PUNCT
bionys-354	125	25	and	and	CCONJ
bionys-354	125	26	2	2	NUM
bionys-354	125	27	concentrations	concentration	NOUN
bionys-354	125	28	of	of	ADP
bionys-354	125	29	hotstarttaq	hotstarttaq	NOUN
bionys-354	125	30	polymerase	polymerase	NOUN
bionys-354	125	31	(	(	PUNCT
bionys-354	125	32	1u	1u	NUM
bionys-354	125	33	and	and	CCONJ
bionys-354	125	34	4u	4u	NOUN
bionys-354	125	35	)	)	PUNCT
bionys-354	125	36	for	for	ADP
bionys-354	125	37	the	the	DET
bionys-354	125	38	capability	capability	NOUN
bionys-354	125	39	of	of	ADP
bionys-354	125	40	alleviating	alleviate	VERB
bionys-354	125	41	pcr	pcr	ADJ
bionys-354	125	42	inhibition	inhibition	NOUN
bionys-354	125	43	and	and	CCONJ
bionys-354	125	44	amplifying	amplifying	ADJ
bionys-354	125	45	methylated	methylate	VERB
bionys-354	125	46	mgmt	mgmt	NOUN
bionys-354	125	47	promoter	promoter	NOUN
bionys-354	125	48	(	(	PUNCT
bionys-354	125	49	81	81	NUM
bionys-354	125	50	bp	bp	NOUN
bionys-354	125	51	)	)	PUNCT
bionys-354	125	52	and	and	CCONJ
bionys-354	125	53	unmethylated	unmethylated	ADJ
bionys-354	125	54	mgmt	mgmt	NOUN
bionys-354	125	55	promoter	promoter	NOUN
bionys-354	125	56	(	(	PUNCT
bionys-354	125	57	93	93	NUM
bionys-354	125	58	bp	bp	NOUN
bionys-354	125	59	)	)	PUNCT
bionys-354	125	60	msp	msp	NOUN
bionys-354	125	61	products	product	NOUN
bionys-354	125	62	.	.	PUNCT
bionys-354	126	1	in	in	ADP
bionys-354	126	2	concordance	concordance	NOUN
bionys-354	126	3	with	with	ADP
bionys-354	126	4	previous	previous	ADJ
bionys-354	126	5	findings	finding	NOUN
bionys-354	126	6	,	,	PUNCT
bionys-354	126	7	this	this	DET
bionys-354	126	8	optimization	optimization	NOUN
bionys-354	126	9	reaction	reaction	NOUN
bionys-354	126	10	revealed	reveal	VERB
bionys-354	126	11	the	the	DET
bionys-354	126	12	4u	4u	NOUN
bionys-354	126	13	of	of	ADP
bionys-354	126	14	hotstarttaq	hotstarttaq	PROPN
bionys-354	126	15	polymerase	polymerase	NOUN
bionys-354	126	16	,	,	PUNCT
bionys-354	126	17	alongside	alongside	ADP
bionys-354	126	18	with	with	ADP
bionys-354	126	19	125	125	NUM
bionys-354	126	20	ng	ng	PROPN
bionys-354	126	21	of	of	ADP
bionys-354	126	22	template	template	NOUN
bionys-354	126	23	dna	dna	NOUN
bionys-354	126	24	as	as	ADP
bionys-354	126	25	optimal	optimal	ADJ
bionys-354	126	26	concentrations	concentration	NOUN
bionys-354	126	27	for	for	ADP
bionys-354	126	28	both	both	CCONJ
bionys-354	126	29	methylated	methylate	VERB
bionys-354	126	30	and	and	CCONJ
bionys-354	126	31	unmethylated	unmethylated	ADJ
bionys-354	126	32	msp	msp	PROPN
bionys-354	126	33	products	product	NOUN
bionys-354	126	34	.	.	PUNCT
bionys-354	127	1	as	as	SCONJ
bionys-354	127	2	seen	see	VERB
bionys-354	127	3	in	in	ADP
bionys-354	127	4	fig	fig	NOUN
bionys-354	127	5	.	.	PUNCT
bionys-354	128	1	2	2	NUM
bionys-354	128	2	,	,	PUNCT
bionys-354	128	3	the	the	DET
bionys-354	128	4	gradual	gradual	ADJ
bionys-354	128	5	increase	increase	NOUN
bionys-354	128	6	of	of	ADP
bionys-354	128	7	template	template	NOUN
bionys-354	128	8	dna	dna	NOUN
bionys-354	128	9	amount	amount	NOUN
bionys-354	128	10	enhances	enhance	VERB
bionys-354	128	11	the	the	DET
bionys-354	128	12	msp	msp	PROPN
bionys-354	128	13	product	product	NOUN
bionys-354	128	14	intensity	intensity	NOUN
bionys-354	128	15	,	,	PUNCT
bionys-354	128	16	both	both	PRON
bionys-354	128	17	in	in	ADP
bionys-354	128	18	1u	1u	NUM
bionys-354	128	19	and	and	CCONJ
bionys-354	128	20	4u	4u	NOUN
bionys-354	128	21	reaction	reaction	NOUN
bionys-354	128	22	subgroups	subgroup	NOUN
bionys-354	128	23	.	.	PUNCT
bionys-354	129	1	this	this	DET
bionys-354	129	2	overcoming	overcoming	NOUN
bionys-354	129	3	of	of	ADP
bionys-354	129	4	pcr	pcr	ADJ
bionys-354	129	5	inhibition	inhibition	NOUN
bionys-354	129	6	occurs	occur	VERB
bionys-354	129	7	through	through	ADP
bionys-354	129	8	increasing	increase	VERB
bionys-354	129	9	the	the	DET
bionys-354	129	10	probability	probability	NOUN
bionys-354	129	11	of	of	ADP
bionys-354	129	12	the	the	DET
bionys-354	129	13	presence	presence	NOUN
bionys-354	129	14	of	of	ADP
bionys-354	129	15	template	template	NOUN
bionys-354	129	16	molecules	molecule	NOUN
bionys-354	129	17	of	of	ADP
bionys-354	129	18	proper	proper	ADJ
bionys-354	129	19	length	length	NOUN
bionys-354	129	20	and	and	CCONJ
bionys-354	129	21	integrity	integrity	NOUN
bionys-354	129	22	.	.	PUNCT
bionys-354	130	1	as	as	ADP
bionys-354	130	2	a	a	DET
bionys-354	130	3	result	result	NOUN
bionys-354	130	4	,	,	PUNCT
bionys-354	130	5	higher	high	ADJ
bionys-354	130	6	template	template	NOUN
bionys-354	130	7	concentrations	concentration	NOUN
bionys-354	130	8	and	and	CCONJ
bionys-354	130	9	1u	1u	NUM
bionys-354	130	10	concentration	concentration	NOUN
bionys-354	130	11	of	of	ADP
bionys-354	130	12	hotstartaq	hotstartaq	NOUN
bionys-354	130	13	polymerase	polymerase	NOUN
bionys-354	130	14	emerged	emerge	VERB
bionys-354	130	15	as	as	ADP
bionys-354	130	16	one	one	NUM
bionys-354	130	17	of	of	ADP
bionys-354	130	18	the	the	DET
bionys-354	130	19	possible	possible	ADJ
bionys-354	130	20	solutions	solution	NOUN
bionys-354	130	21	for	for	ADP
bionys-354	130	22	optimal	optimal	ADJ
bionys-354	130	23	amplification	amplification	NOUN
bionys-354	130	24	conditions	condition	NOUN
bionys-354	130	25	.	.	PUNCT
bionys-354	131	1	furthermore	furthermore	ADV
bionys-354	131	2	,	,	PUNCT
bionys-354	131	3	a	a	DET
bionys-354	131	4	4	4	NUM
bionys-354	131	5	-	-	ADJ
bionys-354	131	6	fold	fold	ADJ
bionys-354	131	7	increase	increase	NOUN
bionys-354	131	8	of	of	ADP
bionys-354	131	9	the	the	DET
bionys-354	131	10	hotstarttaq	hotstarttaq	PROPN
bionys-354	131	11	polymerase	polymerase	NOUN
bionys-354	131	12	clearly	clearly	ADV
bionys-354	131	13	showed	show	VERB
bionys-354	131	14	the	the	DET
bionys-354	131	15	successful	successful	ADJ
bionys-354	131	16	amplification	amplification	NOUN
bionys-354	131	17	in	in	ADP
bionys-354	131	18	replicates	replicate	NOUN
bionys-354	131	19	containing	contain	VERB
bionys-354	131	20	low	low	ADJ
bionys-354	131	21	amounts	amount	NOUN
bionys-354	131	22	of	of	ADP
bionys-354	131	23	template	template	NOUN
bionys-354	131	24	dna	dna	NOUN
bionys-354	131	25	.	.	PUNCT
bionys-354	132	1	the	the	DET
bionys-354	132	2	listed	list	VERB
bionys-354	132	3	observations	observation	NOUN
bionys-354	132	4	were	be	AUX
bionys-354	132	5	suggesting	suggest	VERB
bionys-354	132	6	the	the	DET
bionys-354	132	7	optimal	optimal	ADJ
bionys-354	132	8	amount	amount	NOUN
bionys-354	132	9	of	of	ADP
bionys-354	132	10	125	125	NUM
bionys-354	132	11	ng	ng	NOUN
bionys-354	132	12	and	and	CCONJ
bionys-354	132	13	4u	4u	NOUN
bionys-354	132	14	of	of	ADP
bionys-354	132	15	hotstarttaq	hotstarttaq	NOUN
bionys-354	132	16	polymerase	polymerase	NOUN
bionys-354	132	17	in	in	ADP
bionys-354	132	18	both	both	CCONJ
bionys-354	132	19	methylated	methylate	VERB
bionys-354	132	20	and	and	CCONJ
bionys-354	132	21	unmethylated	unmethylated	ADJ
bionys-354	132	22	mgmt	mgmt	NOUN
bionys-354	132	23	products	product	NOUN
bionys-354	132	24	.	.	PUNCT
bionys-354	133	1	however	however	ADV
bionys-354	133	2	,	,	PUNCT
bionys-354	133	3	the	the	DET
bionys-354	133	4	presence	presence	NOUN
bionys-354	133	5	of	of	ADP
bionys-354	133	6	additional	additional	ADJ
bionys-354	133	7	primer	primer	NOUN
bionys-354	133	8	-	-	PUNCT
bionys-354	133	9	dimer	dimer	NOUN
bionys-354	133	10	bands	band	NOUN
bionys-354	133	11	on	on	ADP
bionys-354	133	12	agarose	agarose	NOUN
bionys-354	133	13	gel	gel	NOUN
bionys-354	133	14	could	could	AUX
bionys-354	133	15	lead	lead	VERB
bionys-354	133	16	to	to	ADP
bionys-354	133	17	misinterpretation	misinterpretation	NOUN
bionys-354	133	18	of	of	ADP
bionys-354	133	19	methylation	methylation	NOUN
bionys-354	133	20	level	level	NOUN
bionys-354	133	21	analysis	analysis	NOUN
bionys-354	133	22	.	.	PUNCT
bionys-354	134	1	to	to	PART
bionys-354	134	2	address	address	VERB
bionys-354	134	3	that	that	DET
bionys-354	134	4	issue	issue	NOUN
bionys-354	134	5	and	and	CCONJ
bionys-354	134	6	further	far	ADV
bionys-354	134	7	elucidate	elucidate	VERB
bionys-354	134	8	optimal	optimal	ADJ
bionys-354	134	9	msp	msp	NOUN
bionys-354	134	10	conditions	condition	NOUN
bionys-354	134	11	,	,	PUNCT
bionys-354	134	12	another	another	DET
bionys-354	134	13	optimization	optimization	NOUN
bionys-354	134	14	reaction	reaction	NOUN
bionys-354	134	15	was	be	AUX
bionys-354	134	16	conducted	conduct	VERB
bionys-354	134	17	.	.	PUNCT
bionys-354	135	1	given	give	VERB
bionys-354	135	2	the	the	DET
bionys-354	135	3	relatively	relatively	ADV
bionys-354	135	4	short	short	ADJ
bionys-354	135	5	length	length	NOUN
bionys-354	135	6	of	of	ADP
bionys-354	135	7	the	the	DET
bionys-354	135	8	mgmt	mgmt	PROPN
bionys-354	135	9	promoter	promoter	PROPN
bionys-354	135	10	msp	msp	PROPN
bionys-354	135	11	products	product	NOUN
bionys-354	135	12	,	,	PUNCT
bionys-354	135	13	this	this	DET
bionys-354	135	14	optimization	optimization	NOUN
bionys-354	135	15	introduced	introduce	VERB
bionys-354	135	16	the	the	DET
bionys-354	135	17	2u	2u	ADJ
bionys-354	135	18	concentration	concentration	NOUN
bionys-354	135	19	of	of	ADP
bionys-354	135	20	hotstarttaq	hotstarttaq	NOUN
bionys-354	135	21	polymerase	polymerase	NOUN
bionys-354	135	22	as	as	ADP
bionys-354	135	23	a	a	DET
bionys-354	135	24	possible	possible	ADJ
bionys-354	135	25	solution	solution	NOUN
bionys-354	135	26	for	for	ADP
bionys-354	135	27	economizing	economize	VERB
bionys-354	135	28	enzyme	enzyme	NOUN
bionys-354	135	29	consumption	consumption	NOUN
bionys-354	135	30	.	.	PUNCT
bionys-354	136	1	for	for	ADP
bionys-354	136	2	that	that	DET
bionys-354	136	3	purpose	purpose	NOUN
bionys-354	136	4	,	,	PUNCT
bionys-354	136	5	optimization	optimization	NOUN
bionys-354	136	6	reaction	reaction	NOUN
bionys-354	136	7	was	be	AUX
bionys-354	136	8	performed	perform	VERB
bionys-354	136	9	including	include	VERB
bionys-354	136	10	3	3	NUM
bionys-354	136	11	concentrations	concentration	NOUN
bionys-354	136	12	of	of	ADP
bionys-354	136	13	hotstartaq	hotstartaq	NOUN
bionys-354	136	14	polymerase	polymerase	NOUN
bionys-354	136	15	(	(	PUNCT
bionys-354	136	16	1u	1u	NUM
bionys-354	136	17	,	,	PUNCT
bionys-354	136	18	2u	2u	NOUN
bionys-354	136	19	,	,	PUNCT
bionys-354	136	20	and	and	CCONJ
bionys-354	136	21	4u	4u	NOUN
bionys-354	136	22	)	)	PUNCT
bionys-354	136	23	,	,	PUNCT
bionys-354	136	24	125	125	NUM
bionys-354	136	25	ng	ng	PROPN
bionys-354	136	26	amount	amount	NOUN
bionys-354	136	27	of	of	ADP
bionys-354	136	28	template	template	NOUN
bionys-354	136	29	dna	dna	NOUN
bionys-354	136	30	,	,	PUNCT
bionys-354	136	31	and	and	CCONJ
bionys-354	136	32	unmethylated	unmethylated	ADJ
bionys-354	136	33	mgmt	mgmt	NOUN
bionys-354	136	34	promoter	promoter	NOUN
bionys-354	136	35	primer	primer	NOUN
bionys-354	136	36	set	set	NOUN
bionys-354	136	37	(	(	PUNCT
bionys-354	136	38	fig	fig	NOUN
bionys-354	136	39	.	.	PUNCT
bionys-354	137	1	biologica	biologica	PROPN
bionys-354	137	2	nyssana	nyssana	PROPN
bionys-354	138	1	●	●	PUNCT
bionys-354	138	2	11	11	NUM
bionys-354	138	3	(	(	PUNCT
bionys-354	138	4	2	2	NUM
bionys-354	138	5	)	)	PUNCT
bionys-354	138	6	december	december	PROPN
bionys-354	138	7	2020	2020	NUM
bionys-354	138	8	:	:	PUNCT
bionys-354	138	9	199	199	NUM
bionys-354	138	10	-	-	SYM
bionys-354	138	11	147	147	NUM
bionys-354	138	12	jovanović	jovanović	NOUN
bionys-354	138	13	et	et	PROPN
bionys-354	138	14	al	al	PROPN
bionys-354	138	15	.	.	PUNCT
bionys-354	139	1	●	●	PUNCT
bionys-354	139	2	optimizing	optimize	VERB
bionys-354	139	3	conditions	condition	NOUN
bionys-354	139	4	for	for	ADP
bionys-354	139	5	mgmt	mgmt	NOUN
bionys-354	139	6	promoter	promoter	NOUN
bionys-354	139	7	methylation	methylation	NOUN
bionys-354	139	8	status	status	NOUN
bionys-354	139	9	analysis	analysis	NOUN
bionys-354	139	10	in	in	ADP
bionys-354	139	11	glioblastoma	glioblastoma	NOUN
bionys-354	139	12	ffpe	ffpe	NOUN
bionys-354	139	13	samples	sample	NOUN
bionys-354	139	14	fig	fig	NOUN
bionys-354	139	15	.	.	PUNCT
bionys-354	140	1	5	5	X
bionys-354	140	2	.	.	X
bionys-354	140	3	differences	difference	NOUN
bionys-354	140	4	in	in	ADP
bionys-354	140	5	mgmt	mgmt	PROPN
bionys-354	140	6	promoter	promoter	NOUN
bionys-354	140	7	methylation	methylation	NOUN
bionys-354	140	8	assessment	assessment	NOUN
bionys-354	140	9	between	between	ADP
bionys-354	140	10	ff	ff	NOUN
bionys-354	140	11	and	and	CCONJ
bionys-354	140	12	ffpe	ffpe	NOUN
bionys-354	140	13	samples	sample	NOUN
bionys-354	140	14	:	:	PUNCT
bionys-354	140	15	a	a	X
bionys-354	140	16	)	)	PUNCT
bionys-354	140	17	agarose	agarose	NOUN
bionys-354	140	18	gel	gel	NOUN
bionys-354	140	19	image	image	NOUN
bionys-354	140	20	presenting	present	VERB
bionys-354	140	21	methylated	methylate	VERB
bionys-354	140	22	and	and	CCONJ
bionys-354	140	23	unmethylated	unmethylated	ADJ
bionys-354	140	24	mgmt	mgmt	PROPN
bionys-354	140	25	promoter	promoter	NOUN
bionys-354	140	26	msp	msp	PROPN
bionys-354	140	27	products	product	NOUN
bionys-354	140	28	with	with	ADP
bionys-354	140	29	the	the	DET
bionys-354	140	30	corresponding	correspond	VERB
bionys-354	140	31	legend	legend	NOUN
bionys-354	140	32	on	on	ADP
bionys-354	140	33	the	the	DET
bionys-354	140	34	right	right	ADJ
bionys-354	140	35	side	side	NOUN
bionys-354	140	36	.	.	PUNCT
bionys-354	141	1	lanes	lane	NOUN
bionys-354	141	2	3	3	NUM
bionys-354	141	3	and	and	CCONJ
bionys-354	141	4	4	4	NUM
bionys-354	141	5	methylated	methylate	VERB
bionys-354	141	6	mgmt	mgmt	NOUN
bionys-354	141	7	promoter	promoter	NOUN
bionys-354	141	8	msp	msp	PROPN
bionys-354	141	9	products	product	NOUN
bionys-354	141	10	involving	involve	VERB
bionys-354	141	11	ffpe	ffpe	NOUN
bionys-354	141	12	samples	sample	NOUN
bionys-354	141	13	of	of	ADP
bionys-354	141	14	the	the	DET
bionys-354	141	15	previously	previously	ADV
bionys-354	141	16	listed	list	VERB
bionys-354	141	17	gbm	gbm	NOUN
bionys-354	141	18	patient	patient	NOUN
bionys-354	141	19	as	as	ADP
bionys-354	141	20	template	template	NOUN
bionys-354	141	21	dna	dna	NOUN
bionys-354	141	22	;	;	PUNCT
bionys-354	141	23	lanes	lane	NOUN
bionys-354	141	24	8	8	NUM
bionys-354	141	25	and	and	CCONJ
bionys-354	141	26	9	9	NUM
bionys-354	141	27	unmethylated	unmethylated	ADJ
bionys-354	141	28	mgmt	mgmt	PROPN
bionys-354	141	29	promoter	promoter	NOUN
bionys-354	141	30	msp	msp	PROPN
bionys-354	141	31	products	product	NOUN
bionys-354	141	32	involving	involve	VERB
bionys-354	141	33	ffpe	ffpe	NOUN
bionys-354	141	34	samples	sample	NOUN
bionys-354	141	35	of	of	ADP
bionys-354	141	36	the	the	DET
bionys-354	141	37	previously	previously	ADV
bionys-354	141	38	listed	list	VERB
bionys-354	141	39	gbm	gbm	NOUN
bionys-354	141	40	patient	patient	NOUN
bionys-354	141	41	as	as	ADP
bionys-354	141	42	template	template	NOUN
bionys-354	141	43	dna	dna	NOUN
bionys-354	141	44	;	;	PUNCT
bionys-354	141	45	lanes	lane	NOUN
bionys-354	141	46	12	12	NUM
bionys-354	141	47	and	and	CCONJ
bionys-354	141	48	13	13	NUM
bionys-354	141	49	reference	reference	NOUN
bionys-354	141	50	ff	ff	PROPN
bionys-354	141	51	sample	sample	NOUN
bionys-354	141	52	mgmt	mgmt	PROPN
bionys-354	141	53	promoter	promoter	NOUN
bionys-354	141	54	msp	msp	PROPN
bionys-354	141	55	products	product	NOUN
bionys-354	141	56	(	(	PUNCT
bionys-354	141	57	methylated	methylate	VERB
bionys-354	141	58	and	and	CCONJ
bionys-354	141	59	unmethylated	unmethylated	ADJ
bionys-354	141	60	)	)	PUNCT
bionys-354	141	61	;	;	PUNCT
bionys-354	141	62	lanes	lane	VERB
bionys-354	141	63	5,6,10,11	5,6,10,11	PROPN
bionys-354	141	64	mgmt	mgmt	NOUN
bionys-354	141	65	promoter	promoter	NOUN
bionys-354	141	66	msp	msp	PROPN
bionys-354	141	67	products	product	NOUN
bionys-354	141	68	(	(	PUNCT
bionys-354	141	69	methylated	methylate	VERB
bionys-354	141	70	and	and	CCONJ
bionys-354	141	71	unmethylated	unmethylated	ADJ
bionys-354	141	72	)	)	PUNCT
bionys-354	141	73	assessed	assess	VERB
bionys-354	141	74	with	with	ADP
bionys-354	141	75	ffpe	ffpe	ADJ
bionys-354	141	76	bisulfite	bisulfite	NOUN
bionys-354	141	77	-	-	PUNCT
bionys-354	141	78	converted	convert	VERB
bionys-354	141	79	sample	sample	NOUN
bionys-354	141	80	of	of	ADP
bionys-354	141	81	different	different	ADJ
bionys-354	141	82	gbm	gbm	NOUN
bionys-354	141	83	patient	patient	NOUN
bionys-354	141	84	.	.	PUNCT
bionys-354	142	1	b	b	X
bionys-354	142	2	)	)	PUNCT
bionys-354	142	3	fluorescence	fluorescence	NOUN
bionys-354	142	4	intensity	intensity	NOUN
bionys-354	142	5	levels	level	NOUN
bionys-354	142	6	of	of	ADP
bionys-354	142	7	methylated	methylate	VERB
bionys-354	142	8	(	(	PUNCT
bionys-354	142	9	m	m	NOUN
bionys-354	142	10	)	)	PUNCT
bionys-354	142	11	and	and	CCONJ
bionys-354	142	12	unmethylated	unmethylated	ADJ
bionys-354	142	13	(	(	PUNCT
bionys-354	142	14	u	u	NOUN
bionys-354	142	15	)	)	PUNCT
bionys-354	142	16	msp	msp	PROPN
bionys-354	142	17	bands	band	NOUN
bionys-354	142	18	obtained	obtain	VERB
bionys-354	142	19	using	use	VERB
bionys-354	142	20	imagej	imagej	ADJ
bionys-354	142	21	software	software	NOUN
bionys-354	142	22	.	.	PUNCT
bionys-354	143	1	products	product	NOUN
bionys-354	143	2	and	and	CCONJ
bionys-354	143	3	ffpe	ffpe	NOUN
bionys-354	143	4	-	-	PUNCT
bionys-354	143	5	derived	derive	VERB
bionys-354	143	6	template	template	NOUN
bionys-354	143	7	dna	dna	NOUN
bionys-354	143	8	.	.	PUNCT
bionys-354	144	1	although	although	SCONJ
bionys-354	144	2	the	the	DET
bionys-354	144	3	above	above	ADV
bionys-354	144	4	-	-	PUNCT
bionys-354	144	5	mentioned	mention	VERB
bionys-354	144	6	pcr	pcr	NOUN
bionys-354	144	7	conditions	condition	NOUN
bionys-354	144	8	refer	refer	VERB
bionys-354	144	9	to	to	ADP
bionys-354	144	10	the	the	DET
bionys-354	144	11	amplification	amplification	NOUN
bionys-354	144	12	of	of	ADP
bionys-354	144	13	specific	specific	ADJ
bionys-354	144	14	mgmt	mgmt	NOUN
bionys-354	144	15	promoter	promoter	NOUN
bionys-354	144	16	pcr	pcr	NOUN
bionys-354	144	17	products	product	NOUN
bionys-354	144	18	using	use	VERB
bionys-354	144	19	the	the	DET
bionys-354	144	20	end	end	NOUN
bionys-354	144	21	-	-	PUNCT
bionys-354	144	22	point	point	NOUN
bionys-354	144	23	pcr	pcr	ADJ
bionys-354	144	24	method	method	NOUN
bionys-354	144	25	,	,	PUNCT
bionys-354	144	26	they	they	PRON
bionys-354	144	27	could	could	AUX
bionys-354	144	28	also	also	ADV
bionys-354	144	29	be	be	AUX
bionys-354	144	30	taken	take	VERB
bionys-354	144	31	into	into	ADP
bionys-354	144	32	consideration	consideration	NOUN
bionys-354	144	33	while	while	SCONJ
bionys-354	144	34	performing	perform	VERB
bionys-354	144	35	ms	ms	ADJ
bionys-354	144	36	-	-	PUNCT
bionys-354	144	37	qpcr	qpcr	ADJ
bionys-354	144	38	reactions	reaction	NOUN
bionys-354	144	39	.	.	PUNCT
bionys-354	145	1	however	however	ADV
bionys-354	145	2	,	,	PUNCT
bionys-354	145	3	it	it	PRON
bionys-354	145	4	should	should	AUX
bionys-354	145	5	be	be	AUX
bionys-354	145	6	noted	note	VERB
bionys-354	145	7	that	that	SCONJ
bionys-354	145	8	due	due	ADP
bionys-354	145	9	to	to	ADP
bionys-354	145	10	the	the	DET
bionys-354	145	11	small	small	ADJ
bionys-354	145	12	sample	sample	NOUN
bionys-354	145	13	size	size	NOUN
bionys-354	145	14	and	and	CCONJ
bionys-354	145	15	a	a	DET
bionys-354	145	16	relatively	relatively	ADV
bionys-354	145	17	small	small	ADJ
bionys-354	145	18	number	number	NOUN
bionys-354	145	19	of	of	ADP
bionys-354	145	20	measurements	measurement	NOUN
bionys-354	145	21	,	,	PUNCT
bionys-354	145	22	the	the	DET
bionys-354	145	23	presented	present	VERB
bionys-354	145	24	results	result	NOUN
bionys-354	145	25	were	be	AUX
bionys-354	145	26	not	not	PART
bionys-354	145	27	additionally	additionally	ADV
bionys-354	145	28	supported	support	VERB
bionys-354	145	29	by	by	ADP
bionys-354	145	30	some	some	DET
bionys-354	145	31	form	form	NOUN
bionys-354	145	32	of	of	ADP
bionys-354	145	33	statistical	statistical	ADJ
bionys-354	145	34	analysis	analysis	NOUN
bionys-354	145	35	.	.	PUNCT
bionys-354	146	1	acknowledgement	acknowledgement	NOUN
bionys-354	146	2	.	.	PUNCT
bionys-354	147	1	this	this	DET
bionys-354	147	2	study	study	NOUN
bionys-354	147	3	was	be	AUX
bionys-354	147	4	supported	support	VERB
bionys-354	147	5	by	by	ADP
bionys-354	147	6	the	the	DET
bionys-354	147	7	ministry	ministry	PROPN
bionys-354	147	8	of	of	ADP
bionys-354	147	9	education	education	PROPN
bionys-354	147	10	,	,	PUNCT
bionys-354	147	11	science	science	NOUN
bionys-354	147	12	and	and	CCONJ
bionys-354	147	13	technological	technological	ADJ
bionys-354	147	14	development	development	NOUN
bionys-354	147	15	of	of	ADP
bionys-354	147	16	the	the	DET
bionys-354	147	17	republic	republic	NOUN
bionys-354	147	18	of	of	ADP
bionys-354	147	19	serbia	serbia	PROPN
bionys-354	147	20	(	(	PUNCT
bionys-354	147	21	contract	contract	NOUN
bionys-354	147	22	number	number	NOUN
bionys-354	147	23	:	:	PUNCT
bionys-354	147	24	451	451	NUM
bionys-354	147	25	-	-	SYM
bionys-354	147	26	03	03	NUM
bionys-354	147	27	-	-	PUNCT
bionys-354	147	28	68/2020	68/2020	NUM
bionys-354	147	29	-	-	PUNCT
bionys-354	147	30	14/200124	14/200124	NUM
bionys-354	147	31	)	)	PUNCT
bionys-354	147	32	.	.	PUNCT
bionys-354	148	1	references	reference	NOUN
bionys-354	148	2	chen	chen	PROPN
bionys-354	148	3	,	,	PUNCT
bionys-354	148	4	r.	r.	PROPN
bionys-354	148	5	,	,	PUNCT
bionys-354	148	6	smith	smith	PROPN
bionys-354	148	7	-	-	PUNCT
bionys-354	148	8	cohn	cohn	PROPN
bionys-354	148	9	,	,	PUNCT
bionys-354	148	10	m.	m.	NOUN
bionys-354	148	11	,	,	PUNCT
bionys-354	148	12	cohen	cohen	PROPN
bionys-354	148	13	,	,	PUNCT
bionys-354	148	14	a.l	a.l	PROPN
bionys-354	148	15	.	.	PROPN
bionys-354	148	16	,	,	PUNCT
bionys-354	148	17	colman	colman	PROPN
bionys-354	148	18	,	,	PUNCT
bionys-354	148	19	h.	h.	PROPN
bionys-354	148	20	2017	2017	NUM
bionys-354	148	21	:	:	PUNCT
bionys-354	148	22	glioma	glioma	NOUN
bionys-354	148	23	subclassifications	subclassification	NOUN
bionys-354	148	24	and	and	CCONJ
bionys-354	148	25	their	their	PRON
bionys-354	148	26	clinical	clinical	ADJ
bionys-354	148	27	significance	significance	NOUN
bionys-354	148	28	.	.	PUNCT
bionys-354	149	1	neurotherapeutics	neurotherapeutic	NOUN
bionys-354	149	2	,	,	PUNCT
bionys-354	149	3	14	14	NUM
bionys-354	149	4	:	:	SYM
bionys-354	149	5	284297	284297	NUM
bionys-354	149	6	.	.	PUNCT
bionys-354	150	1	christians	christians	PROPN
bionys-354	150	2	,	,	PUNCT
bionys-354	150	3	a.	a.	PROPN
bionys-354	150	4	,	,	PUNCT
bionys-354	150	5	hartmann	hartmann	PROPN
bionys-354	150	6	,	,	PUNCT
bionys-354	150	7	c.	c.	PROPN
bionys-354	150	8	,	,	PUNCT
bionys-354	150	9	benner	benner	PROPN
bionys-354	150	10	,	,	PUNCT
bionys-354	150	11	a.	a.	PROPN
bionys-354	150	12	,	,	PUNCT
bionys-354	150	13	meyer	meyer	PROPN
bionys-354	150	14	,	,	PUNCT
bionys-354	150	15	j.	j.	PROPN
bionys-354	150	16	,	,	PUNCT
bionys-354	150	17	von	von	PROPN
bionys-354	150	18	deimling	deimling	PROPN
bionys-354	150	19	,	,	PUNCT
bionys-354	150	20	a.	a.	NOUN
bionys-354	150	21	,	,	PUNCT
bionys-354	150	22	weller	weller	NOUN
bionys-354	150	23	,	,	PUNCT
bionys-354	150	24	m.	m.	NOUN
bionys-354	150	25	,	,	PUNCT
bionys-354	150	26	wick	wick	PROPN
bionys-354	150	27	,	,	PUNCT
bionys-354	150	28	w.	w.	PROPN
bionys-354	150	29	,	,	PUNCT
bionys-354	150	30	weiler	weiler	PROPN
bionys-354	150	31	,	,	PUNCT
bionys-354	150	32	m.	m.	NOUN
bionys-354	150	33	2012	2012	NUM
bionys-354	150	34	:	:	PUNCT
bionys-354	150	35	prognostic	prognostic	ADJ
bionys-354	150	36	value	value	NOUN
bionys-354	150	37	of	of	ADP
bionys-354	150	38	three	three	NUM
bionys-354	150	39	different	different	ADJ
bionys-354	150	40	methods	method	NOUN
bionys-354	150	41	of	of	ADP
bionys-354	150	42	mgmt	mgmt	PROPN
bionys-354	150	43	promoter	promoter	NOUN
bionys-354	150	44	methylation	methylation	NOUN
bionys-354	150	45	analysis	analysis	NOUN
bionys-354	150	46	in	in	ADP
bionys-354	150	47	a	a	DET
bionys-354	150	48	prospective	prospective	ADJ
bionys-354	150	49	trial	trial	NOUN
bionys-354	150	50	on	on	ADP
bionys-354	150	51	newly	newly	ADV
bionys-354	150	52	diagnosed	diagnose	VERB
bionys-354	150	53	glioblastoma	glioblastoma	NOUN
bionys-354	150	54	.	.	PUNCT
bionys-354	151	1	plos	plos	PROPN
bionys-354	151	2	one	one	NUM
bionys-354	151	3	,	,	PUNCT
bionys-354	151	4	7(3	7(3	NUM
bionys-354	151	5	):	):	PUNCT
bionys-354	151	6	e33449	e33449	NOUN
bionys-354	151	7	.	.	PUNCT
bionys-354	151	8	de	de	PROPN
bionys-354	151	9	ruijter	ruijter	PROPN
bionys-354	151	10	,	,	PUNCT
bionys-354	151	11	t.c	t.c	PROPN
bionys-354	151	12	.	.	PROPN
bionys-354	151	13	,	,	PUNCT
bionys-354	151	14	de	de	PROPN
bionys-354	151	15	hoon	hoon	PROPN
bionys-354	151	16	,	,	PUNCT
bionys-354	151	17	j.p	j.p	PROPN
bionys-354	151	18	.	.	PROPN
bionys-354	151	19	,	,	PUNCT
bionys-354	151	20	slaats	slaats	PROPN
bionys-354	151	21	,	,	PUNCT
bionys-354	151	22	j.	j.	PROPN
bionys-354	151	23	,	,	PUNCT
bionys-354	151	24	de	de	PROPN
bionys-354	151	25	vries	vries	PROPN
bionys-354	151	26	,	,	PUNCT
bionys-354	151	27	b.	b.	PROPN
bionys-354	151	28	,	,	PUNCT
bionys-354	151	29	janssen	janssen	PROPN
bionys-354	151	30	,	,	PUNCT
bionys-354	151	31	m.j	m.j	PROPN
bionys-354	151	32	.	.	PROPN
bionys-354	151	33	,	,	PUNCT
bionys-354	151	34	van	van	PROPN
bionys-354	151	35	wezel	wezel	ADJ
bionys-354	151	36	,	,	PUNCT
bionys-354	151	37	t.	t.	NOUN
bionys-354	151	38	,	,	PUNCT
bionys-354	151	39	aarts	aart	NOUN
bionys-354	151	40	,	,	PUNCT
bionys-354	151	41	m.j	m.j	PROPN
bionys-354	151	42	.	.	PROPN
bionys-354	151	43	,	,	PUNCT
bionys-354	151	44	van	van	PROPN
bionys-354	151	45	engeland	engeland	PROPN
bionys-354	151	46	,	,	PUNCT
bionys-354	151	47	m.	m.	NOUN
bionys-354	151	48	,	,	PUNCT
bionys-354	151	49	tjan	tjan	PROPN
bionys-354	151	50	-	-	NOUN
bionys-354	151	51	heijnen	heijnen	NOUN
bionys-354	151	52	,	,	PUNCT
bionys-354	151	53	v.c	v.c	PROPN
bionys-354	151	54	.	.	PROPN
bionys-354	151	55	,	,	PUNCT
bionys-354	151	56	van	van	PROPN
bionys-354	151	57	neste	neste	PROPN
bionys-354	151	58	,	,	PUNCT
bionys-354	151	59	l.	l.	PROPN
bionys-354	151	60	,	,	PUNCT
bionys-354	151	61	veeck	veeck	PROPN
bionys-354	151	62	,	,	PUNCT
bionys-354	151	63	j.	j.	PROPN
bionys-354	151	64	2015	2015	NUM
bionys-354	151	65	:	:	PUNCT
bionys-354	151	66	formalin	formalin	NOUN
bionys-354	151	67	-	-	PUNCT
bionys-354	151	68	fixed	fix	VERB
bionys-354	151	69	,	,	PUNCT
bionys-354	151	70	paraffinembedded	paraffinembedde	VERB
bionys-354	151	71	(	(	PUNCT
bionys-354	151	72	ffpe	ffpe	NOUN
bionys-354	151	73	)	)	PUNCT
bionys-354	151	74	tissueepigenomics	tissueepigenomic	NOUN
bionys-354	151	75	using	use	VERB
bionys-354	151	76	infinium	infinium	NOUN
bionys-354	151	77	humanmethylation450beadchip	humanmethylation450beadchip	NOUN
bionys-354	151	78	assays	assay	NOUN
bionys-354	151	79	.	.	PUNCT
bionys-354	152	1	models	model	NOUN
bionys-354	152	2	and	and	CCONJ
bionys-354	152	3	techniques	technique	NOUN
bionys-354	152	4	,	,	PUNCT
bionys-354	152	5	95	95	NUM
bionys-354	152	6	:	:	PUNCT
bionys-354	152	7	833	833	NUM
bionys-354	152	8	-	-	SYM
bionys-354	152	9	842	842	NUM
bionys-354	152	10	.	.	PUNCT
bionys-354	153	1	dietrich	dietrich	PROPN
bionys-354	153	2	,	,	PUNCT
bionys-354	153	3	d.	d.	PROPN
bionys-354	153	4	,	,	PUNCT
bionys-354	153	5	uhl	uhl	PROPN
bionys-354	153	6	,	,	PUNCT
bionys-354	153	7	b.	b.	PROPN
bionys-354	153	8	,	,	PUNCT
bionys-354	153	9	sailer	sailer	NOUN
bionys-354	153	10	,	,	PUNCT
bionys-354	153	11	v.	v.	PROPN
bionys-354	153	12	,	,	PUNCT
bionys-354	153	13	holmes	holmes	PROPN
bionys-354	153	14	,	,	PUNCT
bionys-354	153	15	e.e	e.e	PROPN
bionys-354	153	16	.	.	PROPN
bionys-354	153	17	,	,	PUNCT
bionys-354	153	18	jung	jung	PROPN
bionys-354	153	19	,	,	PUNCT
bionys-354	153	20	m.	m.	NOUN
bionys-354	153	21	,	,	PUNCT
bionys-354	153	22	meller	meller	PROPN
bionys-354	153	23	,	,	PUNCT
bionys-354	153	24	s.	s.	PROPN
bionys-354	153	25	,	,	PUNCT
bionys-354	153	26	kristiansen	kristiansen	PROPN
bionys-354	153	27	,	,	PUNCT
bionys-354	153	28	g.	g.	PROPN
bionys-354	153	29	2013	2013	NUM
bionys-354	153	30	:	:	PUNCT
bionys-354	153	31	improved	improve	VERB
bionys-354	153	32	pcr	pcr	ADJ
bionys-354	153	33	performance	performance	NOUN
bionys-354	153	34	using	use	VERB
bionys-354	153	35	template	template	NOUN
bionys-354	153	36	dna	dna	NOUN
bionys-354	153	37	from	from	ADP
bionys-354	153	38	formalin	formalin	NOUN
bionys-354	153	39	-	-	PUNCT
bionys-354	153	40	fixed	fix	VERB
bionys-354	153	41	and	and	CCONJ
bionys-354	153	42	paraffin	paraffin	NOUN
bionys-354	153	43	-	-	PUNCT
bionys-354	153	44	embedded	embed	VERB
bionys-354	153	45	tissues	tissue	NOUN
bionys-354	153	46	by	by	ADP
bionys-354	153	47	overcoming	overcome	VERB
bionys-354	153	48	pcr	pcr	ADJ
bionys-354	153	49	inhibition	inhibition	NOUN
bionys-354	153	50	.	.	PUNCT
bionys-354	154	1	plos	plos	PROPN
bionys-354	154	2	one	one	NUM
bionys-354	154	3	,	,	PUNCT
bionys-354	154	4	8(10	8(10	NUM
bionys-354	154	5	):	):	PUNCT
bionys-354	154	6	e77771	e77771	PROPN
bionys-354	154	7	.	.	PUNCT
bionys-354	154	8	esteller	esteller	NOUN
bionys-354	154	9	,	,	PUNCT
bionys-354	154	10	m.	m.	NOUN
bionys-354	154	11	,	,	PUNCT
bionys-354	154	12	garcia	garcia	PROPN
bionys-354	154	13	-	-	PUNCT
bionys-354	154	14	foncillas	foncillas	PROPN
bionys-354	154	15	,	,	PUNCT
bionys-354	154	16	j.	j.	PROPN
bionys-354	154	17	,	,	PUNCT
bionys-354	154	18	andion	andion	NOUN
bionys-354	154	19	,	,	PUNCT
bionys-354	154	20	e.	e.	PROPN
bionys-354	154	21	,	,	PUNCT
bionys-354	154	22	goodman	goodman	PROPN
bionys-354	154	23	,	,	PUNCT
bionys-354	154	24	s.n	s.n	PROPN
bionys-354	154	25	.	.	PROPN
bionys-354	154	26	,	,	PUNCT
bionys-354	154	27	hidalgo	hidalgo	PROPN
bionys-354	154	28	,	,	PUNCT
bionys-354	154	29	o.f	o.f	PROPN
bionys-354	154	30	.	.	PROPN
bionys-354	154	31	,	,	PUNCT
bionys-354	154	32	vanaclocha	vanaclocha	PROPN
bionys-354	154	33	,	,	PUNCT
bionys-354	154	34	v.	v.	PROPN
bionys-354	154	35	,	,	PUNCT
bionys-354	154	36	baylin	baylin	PROPN
bionys-354	154	37	,	,	PUNCT
bionys-354	154	38	s.b	s.b	PROPN
bionys-354	154	39	.	.	PROPN
bionys-354	154	40	,	,	PUNCT
bionys-354	154	41	herman	herman	PROPN
bionys-354	154	42	,	,	PUNCT
bionys-354	154	43	j.g	j.g	PROPN
bionys-354	154	44	.	.	PROPN
bionys-354	154	45	2000	2000	NUM
bionys-354	154	46	:	:	PUNCT
bionys-354	155	1	inactivation	inactivation	NOUN
bionys-354	155	2	of	of	ADP
bionys-354	155	3	the	the	DET
bionys-354	155	4	dna	dna	NOUN
bionys-354	155	5	-	-	PUNCT
bionys-354	155	6	repair	repair	NOUN
bionys-354	155	7	gene	gene	NOUN
bionys-354	155	8	mgmt	mgmt	NOUN
bionys-354	155	9	and	and	CCONJ
bionys-354	155	10	the	the	DET
bionys-354	155	11	clinical	clinical	ADJ
bionys-354	155	12	response	response	NOUN
bionys-354	155	13	of	of	ADP
bionys-354	155	14	gliomas	glioma	NOUN
bionys-354	155	15	to	to	ADP
bionys-354	155	16	alkylating	alkylate	VERB
bionys-354	155	17	agents	agent	NOUN
bionys-354	155	18	.	.	PUNCT
bionys-354	156	1	the	the	DET
bionys-354	156	2	new	new	PROPN
bionys-354	156	3	england	england	PROPN
bionys-354	156	4	journal	journal	PROPN
bionys-354	156	5	of	of	ADP
bionys-354	156	6	medicine	medicine	NOUN
bionys-354	156	7	,	,	PUNCT
bionys-354	156	8	343(19	343(19	NUM
bionys-354	156	9	):	):	PUNCT
bionys-354	156	10	1350	1350	NUM
bionys-354	156	11	-	-	SYM
bionys-354	156	12	1354	1354	NUM
bionys-354	156	13	.	.	PUNCT
bionys-354	157	1	frommer	frommer	NOUN
bionys-354	157	2	,	,	PUNCT
bionys-354	157	3	m.	m.	NOUN
bionys-354	157	4	,	,	PUNCT
bionys-354	157	5	mcdonald	mcdonald	PROPN
bionys-354	157	6	,	,	PUNCT
bionys-354	157	7	l.e	l.e	PROPN
bionys-354	157	8	.	.	PROPN
bionys-354	157	9	,	,	PUNCT
bionys-354	157	10	millar	millar	PROPN
bionys-354	157	11	,	,	PUNCT
bionys-354	157	12	d.s	d.s	PROPN
bionys-354	157	13	.	.	PROPN
bionys-354	157	14	,	,	PUNCT
bionys-354	157	15	collis	collis	PROPN
bionys-354	157	16	,	,	PUNCT
bionys-354	157	17	c.m	c.m	PROPN
bionys-354	157	18	.	.	PROPN
bionys-354	157	19	,	,	PUNCT
bionys-354	157	20	watt	watt	PROPN
bionys-354	157	21	,	,	PUNCT
bionys-354	157	22	f.	f.	PROPN
bionys-354	157	23	,	,	PUNCT
bionys-354	157	24	grigg	grigg	PROPN
bionys-354	157	25	,	,	PUNCT
bionys-354	157	26	g.w	g.w	PROPN
bionys-354	157	27	.	.	PROPN
bionys-354	157	28	,	,	PUNCT
bionys-354	157	29	molloy	molloy	PROPN
bionys-354	157	30	,	,	PUNCT
bionys-354	157	31	p.l	p.l	PROPN
bionys-354	157	32	.	.	PROPN
bionys-354	157	33	,	,	PUNCT
bionys-354	157	34	paul	paul	PROPN
bionys-354	157	35	,	,	PUNCT
bionys-354	157	36	c.l	c.l	PROPN
bionys-354	157	37	.	.	PROPN
bionys-354	157	38	1992	1992	NUM
bionys-354	157	39	:	:	PUNCT
bionys-354	157	40	a	a	DET
bionys-354	157	41	genomic	genomic	NOUN
bionys-354	157	42	sequencing	sequence	VERB
bionys-354	157	43	protocol	protocol	NOUN
bionys-354	157	44	that	that	PRON
bionys-354	157	45	yields	yield	VERB
bionys-354	157	46	a	a	DET
bionys-354	157	47	positive	positive	ADJ
bionys-354	157	48	display	display	NOUN
bionys-354	157	49	of	of	ADP
bionys-354	157	50	5	5	NUM
bionys-354	157	51	-	-	PUNCT
bionys-354	157	52	methylcytosine	methylcytosine	NOUN
bionys-354	157	53	residues	residue	NOUN
bionys-354	157	54	in	in	ADP
bionys-354	157	55	individual	individual	ADJ
bionys-354	157	56	dna	dna	PROPN
bionys-354	157	57	strands	strand	NOUN
bionys-354	157	58	.	.	PUNCT
bionys-354	158	1	proceedings	proceeding	NOUN
bionys-354	158	2	of	of	ADP
bionys-354	158	3	the	the	DET
bionys-354	158	4	national	national	PROPN
bionys-354	158	5	academy	academy	PROPN
bionys-354	158	6	of	of	ADP
bionys-354	158	7	sciences	sciences	PROPN
bionys-354	158	8	of	of	ADP
bionys-354	158	9	the	the	DET
bionys-354	158	10	united	united	PROPN
bionys-354	158	11	states	states	PROPN
bionys-354	158	12	of	of	ADP
bionys-354	158	13	america	america	PROPN
bionys-354	158	14	,	,	PUNCT
bionys-354	158	15	89(5	89(5	NUM
bionys-354	158	16	):	):	PUNCT
bionys-354	158	17	1827	1827	NUM
bionys-354	158	18	-	-	SYM
bionys-354	158	19	1831	1831	NUM
bionys-354	158	20	.	.	PUNCT
bionys-354	159	1	holmes	holmes	PROPN
bionys-354	159	2	,	,	PUNCT
bionys-354	159	3	e.e	e.e	PROPN
bionys-354	159	4	.	.	PROPN
bionys-354	159	5	,	,	PUNCT
bionys-354	159	6	jung	jung	PROPN
bionys-354	159	7	,	,	PUNCT
bionys-354	159	8	m.	m.	NOUN
bionys-354	159	9	,	,	PUNCT
bionys-354	159	10	meller	meller	PROPN
bionys-354	159	11	,	,	PUNCT
bionys-354	159	12	s.	s.	PROPN
bionys-354	159	13	,	,	PUNCT
bionys-354	159	14	leisse	leisse	PROPN
bionys-354	159	15	,	,	PUNCT
bionys-354	159	16	a.	a.	NOUN
bionys-354	159	17	,	,	PUNCT
bionys-354	159	18	146	146	NUM
bionys-354	159	19	biologica	biologica	PROPN
bionys-354	159	20	nyssana	nyssana	PROPN
bionys-354	159	21	●	●	PUNCT
bionys-354	159	22	11	11	NUM
bionys-354	159	23	(	(	PUNCT
bionys-354	159	24	2	2	NUM
bionys-354	159	25	)	)	PUNCT
bionys-354	159	26	december	december	PROPN
bionys-354	159	27	2020	2020	NUM
bionys-354	159	28	:	:	PUNCT
bionys-354	159	29	199	199	NUM
bionys-354	159	30	-	-	SYM
bionys-354	159	31	147	147	NUM
bionys-354	159	32	jovanović	jovanović	NOUN
bionys-354	159	33	et	et	PROPN
bionys-354	159	34	al	al	PROPN
bionys-354	159	35	.	.	PUNCT
bionys-354	160	1	●	●	PUNCT
bionys-354	160	2	optimizing	optimize	VERB
bionys-354	160	3	conditions	condition	NOUN
bionys-354	160	4	for	for	ADP
bionys-354	160	5	mgmt	mgmt	NOUN
bionys-354	160	6	promoter	promoter	NOUN
bionys-354	160	7	methylation	methylation	NOUN
bionys-354	160	8	status	status	NOUN
bionys-354	160	9	analysis	analysis	NOUN
bionys-354	160	10	in	in	ADP
bionys-354	160	11	glioblastoma	glioblastoma	NOUN
bionys-354	160	12	ffpe	ffpe	NOUN
bionys-354	160	13	samples	sample	NOUN
bionys-354	160	14	3	3	NUM
bionys-354	160	15	)	)	PUNCT
bionys-354	160	16	.	.	PUNCT
bionys-354	161	1	ff	ff	INTJ
bionys-354	161	2	dna	dna	NOUN
bionys-354	161	3	bisulfite	bisulfite	NOUN
bionys-354	161	4	-	-	PUNCT
bionys-354	161	5	converted	convert	VERB
bionys-354	161	6	sample	sample	NOUN
bionys-354	161	7	served	serve	VERB
bionys-354	161	8	as	as	ADP
bionys-354	161	9	a	a	DET
bionys-354	161	10	control	control	NOUN
bionys-354	161	11	msp	msp	NOUN
bionys-354	161	12	reaction	reaction	NOUN
bionys-354	161	13	.	.	PUNCT
bionys-354	162	1	despite	despite	SCONJ
bionys-354	162	2	successful	successful	ADJ
bionys-354	162	3	amplification	amplification	NOUN
bionys-354	162	4	of	of	ADP
bionys-354	162	5	the	the	DET
bionys-354	162	6	specific	specific	ADJ
bionys-354	162	7	product	product	NOUN
bionys-354	162	8	in	in	ADP
bionys-354	162	9	1u	1u	NUM
bionys-354	162	10	and	and	CCONJ
bionys-354	162	11	2u	2u	NOUN
bionys-354	162	12	replicates	replicate	NOUN
bionys-354	162	13	,	,	PUNCT
bionys-354	162	14	an	an	DET
bionys-354	162	15	imagej	imagej	ADJ
bionys-354	162	16	software	software	NOUN
bionys-354	162	17	analysis	analysis	NOUN
bionys-354	162	18	showed	show	VERB
bionys-354	162	19	the	the	DET
bionys-354	162	20	significant	significant	ADJ
bionys-354	162	21	presence	presence	NOUN
bionys-354	162	22	of	of	ADP
bionys-354	162	23	primer	primer	NOUN
bionys-354	162	24	-	-	PUNCT
bionys-354	162	25	dimer	dimer	NOUN
bionys-354	162	26	bands	band	NOUN
bionys-354	162	27	in	in	ADP
bionys-354	162	28	1u	1u	NUM
bionys-354	162	29	and	and	CCONJ
bionys-354	162	30	2u	2u	NOUN
bionys-354	162	31	,	,	PUNCT
bionys-354	162	32	but	but	CCONJ
bionys-354	162	33	not	not	PART
bionys-354	162	34	in	in	ADP
bionys-354	162	35	4u	4u	NOUN
bionys-354	162	36	replicate	replicate	VERB
bionys-354	162	37	,	,	PUNCT
bionys-354	162	38	confirming	confirm	VERB
bionys-354	162	39	the	the	DET
bionys-354	162	40	4u	4u	NOUN
bionys-354	162	41	concentration	concentration	NOUN
bionys-354	162	42	of	of	ADP
bionys-354	162	43	hotstartaq	hotstartaq	NOUN
bionys-354	162	44	polymerase	polymerase	NOUN
bionys-354	162	45	as	as	ADP
bionys-354	162	46	the	the	DET
bionys-354	162	47	optimal	optimal	ADJ
bionys-354	162	48	one	one	NOUN
bionys-354	162	49	(	(	PUNCT
bionys-354	162	50	fig	fig	NOUN
bionys-354	162	51	.	.	PUNCT
bionys-354	162	52	4	4	NUM
bionys-354	162	53	)	)	PUNCT
bionys-354	162	54	.	.	PUNCT
bionys-354	163	1	since	since	SCONJ
bionys-354	163	2	the	the	DET
bionys-354	163	3	original	original	ADJ
bionys-354	163	4	study	study	NOUN
bionys-354	163	5	concerning	concern	VERB
bionys-354	163	6	mgmt	mgmt	PROPN
bionys-354	163	7	promoter	promoter	NOUN
bionys-354	163	8	methylation	methylation	NOUN
bionys-354	163	9	in	in	ADP
bionys-354	163	10	gbm	gbm	PROPN
bionys-354	163	11	patients	patient	NOUN
bionys-354	163	12	,	,	PUNCT
bionys-354	163	13	there	there	PRON
bionys-354	163	14	were	be	VERB
bionys-354	163	15	several	several	ADJ
bionys-354	163	16	common	common	ADJ
bionys-354	163	17	approaches	approach	NOUN
bionys-354	163	18	of	of	ADP
bionys-354	163	19	methylation	methylation	NOUN
bionys-354	163	20	status	status	NOUN
bionys-354	163	21	assessment	assessment	NOUN
bionys-354	163	22	(	(	PUNCT
bionys-354	163	23	esteller	esteller	NOUN
bionys-354	163	24	et	et	PROPN
bionys-354	163	25	a.	a.	PROPN
bionys-354	163	26	,	,	PUNCT
bionys-354	163	27	2000	2000	NUM
bionys-354	163	28	)	)	PUNCT
bionys-354	163	29	.	.	PUNCT
bionys-354	164	1	in	in	ADP
bionys-354	164	2	order	order	NOUN
bionys-354	164	3	of	of	ADP
bionys-354	164	4	improving	improve	VERB
bionys-354	164	5	the	the	DET
bionys-354	164	6	qualitative	qualitative	ADJ
bionys-354	164	7	assessment	assessment	NOUN
bionys-354	164	8	results	result	NOUN
bionys-354	164	9	obtained	obtain	VERB
bionys-354	164	10	by	by	ADP
bionys-354	164	11	the	the	DET
bionys-354	164	12	end	end	NOUN
bionys-354	164	13	-	-	PUNCT
bionys-354	164	14	point	point	NOUN
bionys-354	164	15	pcr	pcr	NOUN
bionys-354	164	16	method	method	NOUN
bionys-354	164	17	,	,	PUNCT
bionys-354	164	18	the	the	DET
bionys-354	164	19	semiquantitative	semiquantitative	ADJ
bionys-354	164	20	approach	approach	NOUN
bionys-354	164	21	which	which	PRON
bionys-354	164	22	utilizes	utilize	VERB
bionys-354	164	23	additional	additional	ADJ
bionys-354	164	24	imagej	imagej	ADJ
bionys-354	164	25	software	software	NOUN
bionys-354	164	26	analysis	analysis	NOUN
bionys-354	164	27	of	of	ADP
bionys-354	164	28	gel	gel	NOUN
bionys-354	164	29	images	image	NOUN
bionys-354	164	30	was	be	AUX
bionys-354	164	31	proposed	propose	VERB
bionys-354	164	32	(	(	PUNCT
bionys-354	164	33	dietrich	dietrich	PROPN
bionys-354	164	34	et	et	PROPN
bionys-354	164	35	al	al	PROPN
bionys-354	164	36	.	.	PROPN
bionys-354	164	37	,	,	PUNCT
bionys-354	164	38	2013	2013	NUM
bionys-354	164	39	)	)	PUNCT
bionys-354	164	40	.	.	PUNCT
bionys-354	165	1	thus	thus	ADV
bionys-354	165	2	,	,	PUNCT
bionys-354	165	3	through	through	ADP
bionys-354	165	4	the	the	DET
bionys-354	165	5	measuring	measuring	NOUN
bionys-354	165	6	of	of	ADP
bionys-354	165	7	fluorescence	fluorescence	ADJ
bionys-354	165	8	intensity	intensity	NOUN
bionys-354	165	9	ratios	ratio	NOUN
bionys-354	165	10	of	of	ADP
bionys-354	165	11	methylated	methylate	VERB
bionys-354	165	12	and	and	CCONJ
bionys-354	165	13	unmethylated	unmethylated	ADJ
bionys-354	165	14	mgmt	mgmt	NOUN
bionys-354	165	15	promoter	promoter	NOUN
bionys-354	165	16	product	product	NOUN
bionys-354	165	17	bands	band	NOUN
bionys-354	165	18	,	,	PUNCT
bionys-354	165	19	patients	patient	NOUN
bionys-354	165	20	could	could	AUX
bionys-354	165	21	be	be	AUX
bionys-354	165	22	sorted	sort	VERB
bionys-354	165	23	into	into	ADP
bionys-354	165	24	three	three	NUM
bionys-354	165	25	groups	group	NOUN
bionys-354	165	26	–	–	PUNCT
bionys-354	165	27	unmethylated	unmethylated	ADJ
bionys-354	165	28	(	(	PUNCT
bionys-354	165	29	m	m	NOUN
bionys-354	165	30	/	/	SYM
bionys-354	165	31	u	u	NOUN
bionys-354	165	32	ratio	ratio	NOUN
bionys-354	165	33	=	=	NOUN
bionys-354	165	34	0	0	NUM
bionys-354	165	35	)	)	PUNCT
bionys-354	165	36	,	,	PUNCT
bionys-354	165	37	with	with	ADP
bionys-354	165	38	weak	weak	ADJ
bionys-354	165	39	promoter	promoter	NOUN
bionys-354	165	40	methylation	methylation	NOUN
bionys-354	165	41	(	(	PUNCT
bionys-354	165	42	m	m	NOUN
bionys-354	165	43	/	/	SYM
bionys-354	165	44	u	u	NOUN
bionys-354	165	45	ratio	ratio	NOUN
bionys-354	165	46	between	between	ADP
bionys-354	165	47	0	0	NUM
bionys-354	165	48	and	and	CCONJ
bionys-354	165	49	1	1	NUM
bionys-354	165	50	)	)	PUNCT
bionys-354	165	51	and	and	CCONJ
bionys-354	165	52	strongly	strongly	ADV
bionys-354	165	53	methylated	methylate	VERB
bionys-354	165	54	(	(	PUNCT
bionys-354	165	55	m	m	NOUN
bionys-354	165	56	/	/	SYM
bionys-354	165	57	u	u	NOUN
bionys-354	165	58	ratio	ratio	NOUN
bionys-354	165	59	>	>	X
bionys-354	165	60	1	1	NUM
bionys-354	165	61	)	)	PUNCT
bionys-354	165	62	.	.	PUNCT
bionys-354	166	1	although	although	SCONJ
bionys-354	166	2	this	this	DET
bionys-354	166	3	approach	approach	NOUN
bionys-354	166	4	is	be	AUX
bionys-354	166	5	widely	widely	ADV
bionys-354	166	6	replaced	replace	VERB
bionys-354	166	7	by	by	ADP
bionys-354	166	8	qpcr	qpcr	ADJ
bionys-354	166	9	methods	method	NOUN
bionys-354	166	10	of	of	ADP
bionys-354	166	11	methylation	methylation	NOUN
bionys-354	166	12	status	status	NOUN
bionys-354	166	13	assessment	assessment	NOUN
bionys-354	166	14	,	,	PUNCT
bionys-354	166	15	for	for	ADP
bionys-354	166	16	this	this	DET
bionys-354	166	17	study	study	NOUN
bionys-354	166	18	it	it	PRON
bionys-354	166	19	presents	present	VERB
bionys-354	166	20	a	a	DET
bionys-354	166	21	valuable	valuable	ADJ
bionys-354	166	22	method	method	NOUN
bionys-354	166	23	for	for	ADP
bionys-354	166	24	validation	validation	NOUN
bionys-354	166	25	of	of	ADP
bionys-354	166	26	optimizing	optimize	VERB
bionys-354	166	27	msp	msp	PROPN
bionys-354	166	28	reaction	reaction	NOUN
bionys-354	166	29	results	result	NOUN
bionys-354	166	30	.	.	PUNCT
bionys-354	167	1	by	by	ADP
bionys-354	167	2	comparing	compare	VERB
bionys-354	167	3	m	m	PROPN
bionys-354	167	4	/	/	SYM
bionys-354	167	5	u	u	NOUN
bionys-354	167	6	ratio	ratio	NOUN
bionys-354	167	7	values	value	NOUN
bionys-354	167	8	with	with	ADP
bionys-354	167	9	those	those	PRON
bionys-354	167	10	of	of	ADP
bionys-354	167	11	ff	ff	NOUN
bionys-354	167	12	samples	sample	NOUN
bionys-354	167	13	,	,	PUNCT
bionys-354	167	14	ffpe	ffpe	ADJ
bionys-354	167	15	mgmt	mgmt	PROPN
bionys-354	167	16	promoter	promoter	PROPN
bionys-354	167	17	msp	msp	PROPN
bionys-354	167	18	reaction	reaction	NOUN
bionys-354	167	19	conditions	condition	NOUN
bionys-354	167	20	could	could	AUX
bionys-354	167	21	be	be	AUX
bionys-354	167	22	tested	test	VERB
bionys-354	167	23	.	.	PUNCT
bionys-354	168	1	for	for	ADP
bionys-354	168	2	that	that	DET
bionys-354	168	3	purpose	purpose	NOUN
bionys-354	168	4	,	,	PUNCT
bionys-354	168	5	the	the	DET
bionys-354	168	6	final	final	ADJ
bionys-354	168	7	set	set	NOUN
bionys-354	168	8	of	of	ADP
bionys-354	168	9	mgmt	mgmt	PROPN
bionys-354	168	10	promoter	promoter	PROPN
bionys-354	168	11	msp	msp	PROPN
bionys-354	168	12	reactions	reaction	NOUN
bionys-354	168	13	in	in	ADP
bionys-354	168	14	our	our	PRON
bionys-354	168	15	study	study	NOUN
bionys-354	168	16	have	have	AUX
bionys-354	168	17	included	include	VERB
bionys-354	168	18	both	both	DET
bionys-354	168	19	reference	reference	NOUN
bionys-354	168	20	ff	ff	NOUN
bionys-354	168	21	and	and	CCONJ
bionys-354	168	22	ffpe	ffpe	ADJ
bionys-354	168	23	dna	dna	PROPN
bionys-354	168	24	isolates	isolate	NOUN
bionys-354	168	25	,	,	PUNCT
bionys-354	168	26	performed	perform	VERB
bionys-354	168	27	in	in	ADP
bionys-354	168	28	duplicate	duplicate	NOUN
bionys-354	168	29	with	with	ADP
bionys-354	168	30	previously	previously	ADV
bionys-354	168	31	defined	define	VERB
bionys-354	168	32	pcr	pcr	ADJ
bionys-354	168	33	conditions	condition	NOUN
bionys-354	168	34	(	(	PUNCT
bionys-354	168	35	4u	4u	NOUN
bionys-354	168	36	hotstarttaq	hotstarttaq	VERB
bionys-354	168	37	polymerase	polymerase	NOUN
bionys-354	168	38	and	and	CCONJ
bionys-354	168	39	125	125	NUM
bionys-354	168	40	ng	ng	PROPN
bionys-354	168	41	of	of	ADP
bionys-354	168	42	template	template	NOUN
bionys-354	168	43	dna	dna	PROPN
bionys-354	168	44	)	)	PUNCT
bionys-354	168	45	.	.	PUNCT
bionys-354	169	1	indeed	indeed	ADV
bionys-354	169	2	,	,	PUNCT
bionys-354	169	3	m	m	PROPN
bionys-354	169	4	/	/	SYM
bionys-354	169	5	u	u	NOUN
bionys-354	169	6	ratios	ratio	NOUN
bionys-354	169	7	obtained	obtain	VERB
bionys-354	169	8	for	for	ADP
bionys-354	169	9	corresponding	correspond	VERB
bionys-354	169	10	ff	ff	NOUN
bionys-354	169	11	and	and	CCONJ
bionys-354	169	12	ffpe	ffpe	NOUN
bionys-354	169	13	samples	sample	NOUN
bionys-354	169	14	in	in	ADP
bionys-354	169	15	our	our	PRON
bionys-354	169	16	study	study	NOUN
bionys-354	169	17	were	be	AUX
bionys-354	169	18	very	very	ADV
bionys-354	169	19	similar	similar	ADJ
bionys-354	169	20	,	,	PUNCT
bionys-354	169	21	differing	differ	VERB
bionys-354	169	22	only	only	ADV
bionys-354	169	23	in	in	ADP
bionys-354	169	24	8.47	8.47	NUM
bionys-354	169	25	percent	percent	NOUN
bionys-354	169	26	(	(	PUNCT
bionys-354	169	27	tab	tab	NOUN
bionys-354	169	28	.	.	PUNCT
bionys-354	169	29	4	4	NUM
bionys-354	169	30	)	)	PUNCT
bionys-354	169	31	.	.	PUNCT
bionys-354	170	1	in	in	ADP
bionys-354	170	2	that	that	DET
bionys-354	170	3	manner	manner	NOUN
bionys-354	170	4	both	both	PRON
bionys-354	170	5	of	of	ADP
bionys-354	170	6	the	the	DET
bionys-354	170	7	samples	sample	NOUN
bionys-354	170	8	were	be	AUX
bionys-354	170	9	assessed	assess	VERB
bionys-354	170	10	as	as	ADP
bionys-354	170	11	strongly	strongly	ADV
bionys-354	170	12	methylated	methylate	VERB
bionys-354	170	13	(	(	PUNCT
bionys-354	170	14	tab	tab	NOUN
bionys-354	170	15	.	.	NOUN
bionys-354	170	16	4	4	NUM
bionys-354	170	17	)	)	PUNCT
bionys-354	170	18	,	,	PUNCT
bionys-354	170	19	although	although	SCONJ
bionys-354	170	20	the	the	DET
bionys-354	170	21	absolute	absolute	ADJ
bionys-354	170	22	intensity	intensity	NOUN
bionys-354	170	23	values	value	NOUN
bionys-354	170	24	were	be	AUX
bionys-354	170	25	almost	almost	ADV
bionys-354	170	26	3	3	ADJ
bionys-354	170	27	-	-	ADJ
bionys-354	170	28	fold	fold	ADJ
bionys-354	170	29	different	different	ADJ
bionys-354	170	30	(	(	PUNCT
bionys-354	170	31	fig	fig	NOUN
bionys-354	170	32	.	.	PUNCT
bionys-354	171	1	5b	5b	NUM
bionys-354	171	2	)	)	PUNCT
bionys-354	171	3	.	.	PUNCT
bionys-354	172	1	this	this	DET
bionys-354	172	2	observation	observation	NOUN
bionys-354	172	3	suggests	suggest	VERB
bionys-354	172	4	that	that	SCONJ
bionys-354	172	5	the	the	DET
bionys-354	172	6	optimizing	optimizing	NOUN
bionys-354	172	7	conditions	condition	NOUN
bionys-354	172	8	for	for	ADP
bionys-354	172	9	mgmt	mgmt	PROPN
bionys-354	172	10	promoter	promoter	NOUN
bionys-354	172	11	msp	msp	PROPN
bionys-354	172	12	using	use	VERB
bionys-354	172	13	ffpe	ffpe	NOUN
bionys-354	172	14	-	-	PUNCT
bionys-354	172	15	derived	derive	VERB
bionys-354	172	16	dna	dna	NOUN
bionys-354	172	17	samples	sample	NOUN
bionys-354	172	18	presented	present	VERB
bionys-354	172	19	in	in	ADP
bionys-354	172	20	this	this	DET
bionys-354	172	21	study	study	NOUN
bionys-354	172	22	were	be	AUX
bionys-354	172	23	properly	properly	ADV
bionys-354	172	24	defined	define	VERB
bionys-354	172	25	.	.	PUNCT
bionys-354	173	1	conclusion	conclusion	NOUN
bionys-354	173	2	the	the	DET
bionys-354	173	3	results	result	NOUN
bionys-354	173	4	acquired	acquire	VERB
bionys-354	173	5	in	in	ADP
bionys-354	173	6	our	our	PRON
bionys-354	173	7	study	study	NOUN
bionys-354	173	8	were	be	AUX
bionys-354	173	9	in	in	ADP
bionys-354	173	10	line	line	NOUN
bionys-354	173	11	with	with	ADP
bionys-354	173	12	previous	previous	ADJ
bionys-354	173	13	findings	finding	NOUN
bionys-354	173	14	concerning	concern	VERB
bionys-354	173	15	the	the	DET
bionys-354	173	16	use	use	NOUN
bionys-354	173	17	of	of	ADP
bionys-354	173	18	ffpe	ffpe	ADJ
bionys-354	173	19	tissue	tissue	NOUN
bionys-354	173	20	in	in	ADP
bionys-354	173	21	msp	msp	PROPN
bionys-354	173	22	reactions	reaction	NOUN
bionys-354	173	23	.	.	PUNCT
bionys-354	174	1	thus	thus	ADV
bionys-354	174	2	,	,	PUNCT
bionys-354	174	3	gel	gel	NOUN
bionys-354	174	4	electrophoresis	electrophoresis	NOUN
bionys-354	174	5	analysis	analysis	NOUN
bionys-354	174	6	of	of	ADP
bionys-354	174	7	the	the	DET
bionys-354	174	8	ff	ff	NOUN
bionys-354	174	9	and	and	CCONJ
bionys-354	174	10	ffpe	ffpe	PROPN
bionys-354	174	11	dna	dna	PROPN
bionys-354	174	12	isolates	isolate	NOUN
bionys-354	174	13	confirmed	confirm	VERB
bionys-354	174	14	the	the	DET
bionys-354	174	15	high	high	ADJ
bionys-354	174	16	level	level	NOUN
bionys-354	174	17	of	of	ADP
bionys-354	174	18	degradation	degradation	NOUN
bionys-354	174	19	in	in	ADP
bionys-354	174	20	ffpe	ffpe	ADJ
bionys-354	174	21	isolates	isolate	NOUN
bionys-354	174	22	.	.	PUNCT
bionys-354	175	1	based	base	VERB
bionys-354	175	2	on	on	ADP
bionys-354	175	3	several	several	ADJ
bionys-354	175	4	optimizing	optimizing	NOUN
bionys-354	175	5	reactions	reaction	NOUN
bionys-354	175	6	,	,	PUNCT
bionys-354	175	7	4u	4u	NOUN
bionys-354	175	8	hotstartaq	hotstartaq	NOUN
bionys-354	175	9	polymerase	polymerase	NOUN
bionys-354	175	10	and	and	CCONJ
bionys-354	175	11	125	125	NUM
bionys-354	175	12	ng	ng	PROPN
bionys-354	175	13	of	of	ADP
bionys-354	175	14	template	template	NOUN
bionys-354	175	15	dna	dna	NOUN
bionys-354	175	16	could	could	AUX
bionys-354	175	17	be	be	AUX
bionys-354	175	18	singled	single	VERB
bionys-354	175	19	out	out	ADP
bionys-354	175	20	with	with	ADP
bionys-354	175	21	high	high	ADJ
bionys-354	175	22	certainty	certainty	NOUN
bionys-354	175	23	as	as	ADP
bionys-354	175	24	suitable	suitable	ADJ
bionys-354	175	25	concentrations	concentration	NOUN
bionys-354	175	26	for	for	ADP
bionys-354	175	27	successful	successful	ADJ
bionys-354	175	28	msp	msp	PROPN
bionys-354	175	29	reaction	reaction	NOUN
bionys-354	175	30	concerning	concern	VERB
bionys-354	175	31	methylated	methylate	VERB
bionys-354	175	32	and	and	CCONJ
bionys-354	175	33	unmethylated	unmethylated	ADJ
bionys-354	175	34	mgmt	mgmt	NOUN
bionys-354	175	35	promoter	promoter	NOUN
bionys-354	175	36	amplification	amplification	NOUN
bionys-354	176	1	biologica	biologica	PROPN
bionys-354	176	2	nyssana	nyssana	PROPN
bionys-354	176	3	●	●	PUNCT
bionys-354	176	4	11	11	NUM
bionys-354	176	5	(	(	PUNCT
bionys-354	176	6	2	2	NUM
bionys-354	176	7	)	)	PUNCT
bionys-354	176	8	december	december	PROPN
bionys-354	176	9	2020	2020	NUM
bionys-354	176	10	:	:	PUNCT
bionys-354	176	11	199	199	NUM
bionys-354	176	12	-	-	SYM
bionys-354	176	13	147	147	NUM
bionys-354	176	14	jovanović	jovanović	NOUN
bionys-354	176	15	et	et	PROPN
bionys-354	176	16	al	al	PROPN
bionys-354	176	17	.	.	PUNCT
bionys-354	177	1	●	●	PUNCT
bionys-354	177	2	optimizing	optimize	VERB
bionys-354	177	3	conditions	condition	NOUN
bionys-354	177	4	for	for	ADP
bionys-354	177	5	mgmt	mgmt	NOUN
bionys-354	177	6	promoter	promoter	NOUN
bionys-354	177	7	methylation	methylation	NOUN
bionys-354	177	8	status	status	NOUN
bionys-354	177	9	analysis	analysis	NOUN
bionys-354	177	10	in	in	ADP
bionys-354	177	11	glioblastoma	glioblastoma	NOUN
bionys-354	177	12	ffpe	ffpe	NOUN
bionys-354	177	13	samples	sample	NOUN
bionys-354	177	14	sailer	sailer	NOUN
bionys-354	177	15	,	,	PUNCT
bionys-354	177	16	v.	v.	ADV
bionys-354	177	17	,	,	PUNCT
bionys-354	177	18	zech	zech	PROPN
bionys-354	177	19	,	,	PUNCT
bionys-354	177	20	j.	j.	PROPN
bionys-354	177	21	,	,	PUNCT
bionys-354	177	22	mengdehl	mengdehl	PROPN
bionys-354	177	23	,	,	PUNCT
bionys-354	177	24	m.	m.	NOUN
bionys-354	177	25	,	,	PUNCT
bionys-354	177	26	garbe	garbe	PROPN
bionys-354	177	27	,	,	PUNCT
bionys-354	177	28	l.a	l.a	PROPN
bionys-354	177	29	.	.	PROPN
bionys-354	177	30	,	,	PUNCT
bionys-354	177	31	uhl	uhl	PROPN
bionys-354	177	32	,	,	PUNCT
bionys-354	177	33	b.	b.	PROPN
bionys-354	177	34	,	,	PUNCT
bionys-354	177	35	kristiansen	kristiansen	PROPN
bionys-354	177	36	,	,	PUNCT
bionys-354	177	37	g.	g.	PROPN
bionys-354	177	38	,	,	PUNCT
bionys-354	177	39	dietrich	dietrich	PROPN
bionys-354	177	40	,	,	PUNCT
bionys-354	177	41	d.	d.	PROPN
bionys-354	177	42	2014	2014	NUM
bionys-354	177	43	:	:	PUNCT
bionys-354	177	44	performance	performance	NOUN
bionys-354	177	45	evaluation	evaluation	NOUN
bionys-354	177	46	of	of	ADP
bionys-354	177	47	kits	kit	NOUN
bionys-354	177	48	for	for	ADP
bionys-354	177	49	bisulfiteconversion	bisulfiteconversion	NOUN
bionys-354	177	50	of	of	ADP
bionys-354	177	51	dna	dna	PROPN
bionys-354	177	52	from	from	ADP
bionys-354	177	53	tissues	tissue	NOUN
bionys-354	177	54	,	,	PUNCT
bionys-354	177	55	cell	cell	NOUN
bionys-354	177	56	lines	line	NOUN
bionys-354	177	57	,	,	PUNCT
bionys-354	177	58	ffpe	ffpe	NOUN
bionys-354	177	59	tissues	tissue	NOUN
bionys-354	177	60	,	,	PUNCT
bionys-354	177	61	aspirates	aspirate	NOUN
bionys-354	177	62	,	,	PUNCT
bionys-354	177	63	lavages	lavage	NOUN
bionys-354	177	64	,	,	PUNCT
bionys-354	177	65	effusions	effusion	NOUN
bionys-354	177	66	,	,	PUNCT
bionys-354	177	67	plasma	plasma	NOUN
bionys-354	177	68	,	,	PUNCT
bionys-354	177	69	serum	serum	NOUN
bionys-354	177	70	,	,	PUNCT
bionys-354	177	71	and	and	CCONJ
bionys-354	177	72	urine	urine	NOUN
bionys-354	177	73	.	.	PUNCT
bionys-354	178	1	plos	plos	PROPN
bionys-354	178	2	one	one	NUM
bionys-354	178	3	,	,	PUNCT
bionys-354	178	4	9(4	9(4	NUM
bionys-354	178	5	):	):	PUNCT
bionys-354	178	6	e93933	e93933	PROPN
bionys-354	178	7	.	.	PUNCT
bionys-354	178	8	kint	kint	PROPN
bionys-354	178	9	,	,	PUNCT
bionys-354	178	10	s.	s.	PROPN
bionys-354	178	11	,	,	PUNCT
bionys-354	178	12	de	de	PROPN
bionys-354	178	13	spiegelaere	spiegelaere	PROPN
bionys-354	178	14	,	,	PUNCT
bionys-354	178	15	w.	w.	PROPN
bionys-354	178	16	,	,	PUNCT
bionys-354	178	17	de	de	PROPN
bionys-354	178	18	kesel	kesel	PROPN
bionys-354	178	19	,	,	PUNCT
bionys-354	178	20	j.	j.	PROPN
bionys-354	178	21	,	,	PUNCT
bionys-354	178	22	vandekerckhove	vandekerckhove	NOUN
bionys-354	178	23	,	,	PUNCT
bionys-354	178	24	l.	l.	PROPN
bionys-354	178	25	,	,	PUNCT
bionys-354	178	26	van	van	PROPN
bionys-354	178	27	criekinge	criekinge	NOUN
bionys-354	178	28	,	,	PUNCT
bionys-354	178	29	w.	w.	NOUN
bionys-354	178	30	2018	2018	NUM
bionys-354	178	31	:	:	PUNCT
bionys-354	178	32	evaluation	evaluation	NOUN
bionys-354	178	33	of	of	ADP
bionys-354	178	34	bisulfite	bisulfite	NOUN
bionys-354	178	35	kits	kit	NOUN
bionys-354	178	36	for	for	ADP
bionys-354	178	37	dna	dna	PROPN
bionys-354	178	38	methylation	methylation	NOUN
bionys-354	178	39	profiling	profiling	NOUN
bionys-354	178	40	in	in	ADP
bionys-354	178	41	terms	term	NOUN
bionys-354	178	42	of	of	ADP
bionys-354	178	43	dna	dna	PROPN
bionys-354	178	44	fragmentation	fragmentation	NOUN
bionys-354	178	45	and	and	CCONJ
bionys-354	178	46	dna	dna	PROPN
bionys-354	178	47	recovery	recovery	NOUN
bionys-354	178	48	using	use	VERB
bionys-354	178	49	digital	digital	ADJ
bionys-354	178	50	pcr	pcr	NOUN
bionys-354	178	51	.	.	PUNCT
bionys-354	179	1	plos	plos	PROPN
bionys-354	179	2	one	one	NUM
bionys-354	179	3	,	,	PUNCT
bionys-354	179	4	13(6	13(6	PROPN
bionys-354	179	5	):	):	PUNCT
bionys-354	179	6	e0199091	e0199091	PROPN
bionys-354	179	7	.	.	PROPN
bionys-354	179	8	louis	louis	PROPN
bionys-354	179	9	,	,	PUNCT
bionys-354	179	10	d.n	d.n	PROPN
bionys-354	179	11	.	.	PROPN
bionys-354	179	12	,	,	PUNCT
bionys-354	179	13	perry	perry	PROPN
bionys-354	179	14	,	,	PUNCT
bionys-354	179	15	a.	a.	NOUN
bionys-354	179	16	,	,	PUNCT
bionys-354	179	17	reifenberger	reifenberger	NOUN
bionys-354	179	18	,	,	PUNCT
bionys-354	179	19	g.	g.	PROPN
bionys-354	179	20	,	,	PUNCT
bionys-354	179	21	von	von	PROPN
bionys-354	179	22	deimling	deimling	PROPN
bionys-354	179	23	,	,	PUNCT
bionys-354	179	24	a.	a.	NOUN
bionys-354	179	25	,	,	PUNCT
bionys-354	179	26	figarella	figarella	NOUN
bionys-354	179	27	-	-	PUNCT
bionys-354	179	28	branger	branger	NOUN
bionys-354	179	29	,	,	PUNCT
bionys-354	179	30	d.	d.	PROPN
bionys-354	179	31	,	,	PUNCT
bionys-354	179	32	cavenee	cavenee	PROPN
bionys-354	179	33	,	,	PUNCT
bionys-354	179	34	w.k	w.k	PROPN
bionys-354	179	35	.	.	PROPN
bionys-354	179	36	,	,	PUNCT
bionys-354	179	37	ohgaki	ohgaki	PROPN
bionys-354	179	38	,	,	PUNCT
bionys-354	179	39	h.	h.	PROPN
bionys-354	179	40	,	,	PUNCT
bionys-354	179	41	wiestler	wiestler	PROPN
bionys-354	179	42	,	,	PUNCT
bionys-354	179	43	o.d	o.d	PROPN
bionys-354	179	44	.	.	PROPN
bionys-354	179	45	,	,	PUNCT
bionys-354	179	46	kleihues	kleihue	NOUN
bionys-354	179	47	,	,	PUNCT
bionys-354	179	48	p.	p.	PROPN
bionys-354	179	49	,	,	PUNCT
bionys-354	179	50	ellison	ellison	PROPN
bionys-354	179	51	,	,	PUNCT
bionys-354	179	52	d.w	d.w	PROPN
bionys-354	179	53	.	.	PROPN
bionys-354	179	54	2016	2016	NUM
bionys-354	179	55	:	:	PUNCT
bionys-354	179	56	the	the	DET
bionys-354	179	57	2016	2016	NUM
bionys-354	179	58	world	world	PROPN
bionys-354	179	59	health	health	NOUN
bionys-354	179	60	organization	organization	NOUN
bionys-354	179	61	classification	classification	NOUN
bionys-354	179	62	of	of	ADP
bionys-354	179	63	tumors	tumor	NOUN
bionys-354	179	64	of	of	ADP
bionys-354	179	65	the	the	DET
bionys-354	179	66	central	central	ADJ
bionys-354	179	67	nervous	nervous	ADJ
bionys-354	179	68	system	system	NOUN
bionys-354	179	69	:	:	PUNCT
bionys-354	179	70	a	a	DET
bionys-354	179	71	summary	summary	NOUN
bionys-354	179	72	.	.	PUNCT
bionys-354	180	1	acta	acta	PROPN
bionys-354	180	2	neuropathologica	neuropathologica	PROPN
bionys-354	180	3	,	,	PUNCT
bionys-354	180	4	131(6	131(6	NUM
bionys-354	180	5	):	):	PUNCT
bionys-354	180	6	803	803	NUM
bionys-354	180	7	-	-	SYM
bionys-354	180	8	820	820	NUM
bionys-354	180	9	.	.	PUNCT
bionys-354	181	1	ludgate	ludgate	PROPN
bionys-354	181	2	,	,	PUNCT
bionys-354	181	3	j.l	j.l	PROPN
bionys-354	181	4	.	.	PROPN
bionys-354	181	5	,	,	PUNCT
bionys-354	181	6	wright	wright	PROPN
bionys-354	181	7	,	,	PUNCT
bionys-354	181	8	j.	j.	PROPN
bionys-354	181	9	,	,	PUNCT
bionys-354	181	10	stockwell	stockwell	PROPN
bionys-354	181	11	,	,	PUNCT
bionys-354	181	12	p.a	p.a	PROPN
bionys-354	181	13	.	.	PROPN
bionys-354	181	14	,	,	PUNCT
bionys-354	181	15	morison	morison	PROPN
bionys-354	181	16	,	,	PUNCT
bionys-354	181	17	i.m	i.m	PROPN
bionys-354	181	18	.	.	PROPN
bionys-354	181	19	,	,	PUNCT
bionys-354	181	20	eccles	eccles	PROPN
bionys-354	181	21	,	,	PUNCT
bionys-354	181	22	m.r	m.r	PROPN
bionys-354	181	23	.	.	PROPN
bionys-354	181	24	,	,	PUNCT
bionys-354	181	25	chatterjee	chatterjee	PROPN
bionys-354	181	26	,	,	PUNCT
bionys-354	181	27	a.	a.	NOUN
bionys-354	181	28	2017	2017	NUM
bionys-354	181	29	:	:	PUNCT
bionys-354	181	30	a	a	DET
bionys-354	181	31	streamlined	streamlined	ADJ
bionys-354	181	32	method	method	NOUN
bionys-354	181	33	for	for	ADP
bionys-354	181	34	analysing	analyse	VERB
bionys-354	181	35	genome	genome	NOUN
bionys-354	181	36	-	-	PUNCT
bionys-354	181	37	wide	wide	ADJ
bionys-354	181	38	dna	dna	PROPN
bionys-354	181	39	methylation	methylation	NOUN
bionys-354	181	40	patterns	pattern	NOUN
bionys-354	181	41	from	from	ADP
bionys-354	181	42	low	low	ADJ
bionys-354	181	43	amounts	amount	NOUN
bionys-354	181	44	of	of	ADP
bionys-354	181	45	ffpe	ffpe	PROPN
bionys-354	181	46	dna	dna	PROPN
bionys-354	181	47	.	.	PUNCT
bionys-354	182	1	bmc	bmc	PROPN
bionys-354	182	2	medical	medical	ADJ
bionys-354	182	3	genomics	genomic	NOUN
bionys-354	182	4	,	,	PUNCT
bionys-354	182	5	10(1	10(1	NUM
bionys-354	182	6	):	):	PUNCT
bionys-354	182	7	54	54	NUM
bionys-354	182	8	.	.	PUNCT
bionys-354	183	1	patterson	patterson	PROPN
bionys-354	183	2	,	,	PUNCT
bionys-354	183	3	k.	k.	PROPN
bionys-354	183	4	,	,	PUNCT
bionys-354	183	5	molloy	molloy	PROPN
bionys-354	183	6	,	,	PUNCT
bionys-354	183	7	l.	l.	PROPN
bionys-354	183	8	,	,	PUNCT
bionys-354	183	9	qu	qu	PROPN
bionys-354	183	10	,	,	PUNCT
bionys-354	183	11	w.	w.	PROPN
bionys-354	183	12	,	,	PUNCT
bionys-354	183	13	clark	clark	PROPN
bionys-354	183	14	,	,	PUNCT
bionys-354	183	15	s.	s.	PROPN
bionys-354	183	16	2011	2011	NUM
bionys-354	183	17	:	:	PUNCT
bionys-354	183	18	dna	dna	PROPN
bionys-354	183	19	methylation	methylation	NOUN
bionys-354	183	20	:	:	PUNCT
bionys-354	183	21	bisulphite	bisulphite	ADJ
bionys-354	183	22	modification	modification	NOUN
bionys-354	183	23	and	and	CCONJ
bionys-354	183	24	analysis	analysis	NOUN
bionys-354	183	25	.	.	PUNCT
bionys-354	184	1	journal	journal	NOUN
bionys-354	184	2	of	of	ADP
bionys-354	184	3	visualized	visualized	ADJ
bionys-354	184	4	experiments	experiment	NOUN
bionys-354	184	5	,	,	PUNCT
bionys-354	184	6	(	(	PUNCT
bionys-354	184	7	56	56	NUM
bionys-354	184	8	):	):	PUNCT
bionys-354	184	9	3170	3170	NUM
bionys-354	184	10	.	.	PUNCT
bionys-354	185	1	sengüven	sengüven	PROPN
bionys-354	185	2	,	,	PUNCT
bionys-354	185	3	b.	b.	PROPN
bionys-354	185	4	,	,	PUNCT
bionys-354	185	5	baris	baris	PROPN
bionys-354	185	6	,	,	PUNCT
bionys-354	185	7	e.	e.	PROPN
bionys-354	185	8	,	,	PUNCT
bionys-354	185	9	oygur	oygur	PROPN
bionys-354	185	10	,	,	PUNCT
bionys-354	185	11	t.	t.	PROPN
bionys-354	185	12	,	,	PUNCT
bionys-354	185	13	berktas	berkta	NOUN
bionys-354	185	14	,	,	PUNCT
bionys-354	185	15	m.	m.	NOUN
bionys-354	185	16	2014	2014	NUM
bionys-354	185	17	:	:	PUNCT
bionys-354	185	18	comparison	comparison	NOUN
bionys-354	185	19	of	of	ADP
bionys-354	185	20	methods	method	NOUN
bionys-354	185	21	for	for	ADP
bionys-354	185	22	the	the	DET
bionys-354	185	23	extraction	extraction	NOUN
bionys-354	185	24	of	of	ADP
bionys-354	185	25	dna	dna	NOUN
bionys-354	185	26	from	from	ADP
bionys-354	185	27	formalin	formalin	NOUN
bionys-354	185	28	-	-	PUNCT
bionys-354	185	29	fixed	fix	VERB
bionys-354	185	30	,	,	PUNCT
bionys-354	185	31	paraffin	paraffin	NOUN
bionys-354	185	32	-	-	PUNCT
bionys-354	185	33	embedded	embed	VERB
bionys-354	185	34	archival	archival	NOUN
bionys-354	185	35	tissues	tissue	NOUN
bionys-354	185	36	.	.	PUNCT
bionys-354	186	1	international	international	ADJ
bionys-354	186	2	journal	journal	PROPN
bionys-354	186	3	of	of	ADP
bionys-354	186	4	medical	medical	ADJ
bionys-354	186	5	sciences	science	NOUN
bionys-354	186	6	,	,	PUNCT
bionys-354	186	7	11(5	11(5	NUM
bionys-354	186	8	):	):	PUNCT
bionys-354	186	9	494	494	NUM
bionys-354	186	10	-	-	SYM
bionys-354	186	11	499	499	NUM
bionys-354	186	12	.	.	PUNCT
bionys-354	187	1	soomro	soomro	PROPN
bionys-354	187	2	,	,	PUNCT
bionys-354	187	3	s.h	s.h	PROPN
bionys-354	187	4	.	.	PROPN
bionys-354	187	5	,	,	PUNCT
bionys-354	187	6	ting	ting	PROPN
bionys-354	187	7	,	,	PUNCT
bionys-354	187	8	l.r	l.r	PROPN
bionys-354	187	9	.	.	PROPN
bionys-354	187	10	,	,	PUNCT
bionys-354	187	11	qing	qing	NOUN
bionys-354	187	12	,	,	PUNCT
bionys-354	187	13	y.y	y.y	PROPN
bionys-354	187	14	.	.	PROPN
bionys-354	187	15	,	,	PUNCT
bionys-354	187	16	ren	ren	PROPN
bionys-354	187	17	,	,	PUNCT
bionys-354	187	18	m.	m.	NOUN
bionys-354	187	19	2017	2017	NUM
bionys-354	187	20	:	:	PUNCT
bionys-354	187	21	molecular	molecular	ADJ
bionys-354	187	22	biology	biology	NOUN
bionys-354	187	23	of	of	ADP
bionys-354	187	24	glioblastoma	glioblastoma	NOUN
bionys-354	187	25	:	:	PUNCT
bionys-354	187	26	classification	classification	NOUN
bionys-354	187	27	and	and	CCONJ
bionys-354	187	28	mutational	mutational	ADJ
bionys-354	187	29	locations	location	NOUN
bionys-354	187	30	.	.	PUNCT
bionys-354	188	1	the	the	DET
bionys-354	188	2	journal	journal	NOUN
bionys-354	188	3	of	of	ADP
bionys-354	188	4	the	the	DET
bionys-354	188	5	pakistan	pakistan	PROPN
bionys-354	188	6	medical	medical	PROPN
bionys-354	188	7	association	association	PROPN
bionys-354	188	8	,	,	PUNCT
bionys-354	188	9	67(9	67(9	NOUN
bionys-354	188	10	):	):	PUNCT
bionys-354	188	11	14101414	14101414	NUM
bionys-354	188	12	.	.	PUNCT
bionys-354	189	1	thon	thon	PROPN
bionys-354	189	2	,	,	PUNCT
bionys-354	189	3	n.	n.	NOUN
bionys-354	189	4	,	,	PUNCT
bionys-354	189	5	eigenbrod	eigenbrod	PROPN
bionys-354	189	6	,	,	PUNCT
bionys-354	189	7	s.	s.	PROPN
bionys-354	189	8	,	,	PUNCT
bionys-354	189	9	grasbon	grasbon	NOUN
bionys-354	189	10	-	-	PUNCT
bionys-354	189	11	frodl	frodl	ADJ
bionys-354	189	12	,	,	PUNCT
bionys-354	189	13	e.m	e.m	PROPN
bionys-354	189	14	.	.	PROPN
bionys-354	189	15	,	,	PUNCT
bionys-354	189	16	lutz	lutz	PROPN
bionys-354	189	17	,	,	PUNCT
bionys-354	189	18	j.	j.	PROPN
bionys-354	189	19	,	,	PUNCT
bionys-354	189	20	kreth	kreth	PROPN
bionys-354	189	21	,	,	PUNCT
bionys-354	189	22	s.	s.	PROPN
bionys-354	189	23	,	,	PUNCT
bionys-354	189	24	popperl	popperl	PROPN
bionys-354	189	25	,	,	PUNCT
bionys-354	189	26	g.	g.	NOUN
bionys-354	189	27	,	,	PUNCT
bionys-354	189	28	belka	belka	NOUN
bionys-354	189	29	,	,	PUNCT
bionys-354	189	30	c.	c.	PROPN
bionys-354	189	31	,	,	PUNCT
bionys-354	189	32	kretzschmar	kretzschmar	PROPN
bionys-354	189	33	,	,	PUNCT
bionys-354	189	34	h.a	h.a	PROPN
bionys-354	189	35	.	.	PROPN
bionys-354	189	36	,	,	PUNCT
bionys-354	189	37	tonn	tonn	PROPN
bionys-354	189	38	,	,	PUNCT
bionys-354	189	39	j.c	j.c	PROPN
bionys-354	189	40	.	.	PROPN
bionys-354	189	41	,	,	PUNCT
bionys-354	189	42	kreth	kreth	PROPN
bionys-354	189	43	,	,	PUNCT
bionys-354	189	44	f.w	f.w	PROPN
bionys-354	189	45	.	.	PROPN
bionys-354	189	46	2011	2011	NUM
bionys-354	189	47	:	:	PUNCT
bionys-354	189	48	predominant	predominant	ADJ
bionys-354	189	49	influence	influence	NOUN
bionys-354	189	50	of	of	ADP
bionys-354	189	51	mgmt	mgmt	PROPN
bionys-354	189	52	methylation	methylation	NOUN
bionys-354	189	53	in	in	ADP
bionys-354	189	54	non	non	ADJ
bionys-354	189	55	-	-	ADJ
bionys-354	189	56	resectable	resectable	ADJ
bionys-354	189	57	glioblastoma	glioblastoma	NOUN
bionys-354	189	58	after	after	ADP
bionys-354	189	59	radiotherapy	radiotherapy	NOUN
bionys-354	189	60	plus	plus	CCONJ
bionys-354	189	61	temozolomide	temozolomide	NOUN
bionys-354	189	62	.	.	PUNCT
bionys-354	190	1	journal	journal	PROPN
bionys-354	190	2	of	of	ADP
bionys-354	190	3	neurology	neurology	NOUN
bionys-354	190	4	,	,	PUNCT
bionys-354	190	5	neurosurgery	neurosurgery	NOUN
bionys-354	190	6	,	,	PUNCT
bionys-354	190	7	and	and	CCONJ
bionys-354	190	8	psychiatry	psychiatry	NOUN
bionys-354	190	9	,	,	PUNCT
bionys-354	190	10	82(4	82(4	NUM
bionys-354	190	11	):	):	PUNCT
bionys-354	190	12	441–446	441–446	NUM
bionys-354	190	13	.	.	PUNCT
bionys-354	191	1	thon	thon	PROPN
bionys-354	191	2	,	,	PUNCT
bionys-354	191	3	n.	n.	NOUN
bionys-354	191	4	,	,	PUNCT
bionys-354	191	5	kreth	kreth	PROPN
bionys-354	191	6	,	,	PUNCT
bionys-354	191	7	s.	s.	PROPN
bionys-354	191	8	,	,	PUNCT
bionys-354	191	9	kreth	kreth	PROPN
bionys-354	191	10	,	,	PUNCT
bionys-354	191	11	f.w	f.w	PROPN
bionys-354	191	12	.	.	PROPN
bionys-354	191	13	2013	2013	NUM
bionys-354	191	14	:	:	PUNCT
bionys-354	191	15	personalized	personalized	ADJ
bionys-354	191	16	treatment	treatment	NOUN
bionys-354	191	17	strategies	strategy	NOUN
bionys-354	191	18	in	in	ADP
bionys-354	191	19	glioblastoma	glioblastoma	NOUN
bionys-354	191	20	:	:	PUNCT
bionys-354	191	21	mgmt	mgmt	NOUN
bionys-354	191	22	promoter	promoter	NOUN
bionys-354	191	23	methylation	methylation	NOUN
bionys-354	191	24	status	status	NOUN
bionys-354	191	25	.	.	PUNCT
bionys-354	192	1	onco	onco	NOUN
bionys-354	192	2	targets	target	NOUN
bionys-354	192	3	and	and	CCONJ
bionys-354	192	4	therapy	therapy	NOUN
bionys-354	192	5	,	,	PUNCT
bionys-354	192	6	6	6	NUM
bionys-354	192	7	:	:	SYM
bionys-354	192	8	1363	1363	NUM
bionys-354	192	9	-	-	SYM
bionys-354	192	10	72	72	NUM
bionys-354	192	11	.	.	PUNCT
bionys-354	193	1	tournier	tournier	PROPN
bionys-354	193	2	,	,	PUNCT
bionys-354	193	3	b.	b.	PROPN
bionys-354	193	4	,	,	PUNCT
bionys-354	193	5	chapusot	chapusot	NOUN
bionys-354	193	6	,	,	PUNCT
bionys-354	193	7	c.	c.	PROPN
bionys-354	193	8	,	,	PUNCT
bionys-354	193	9	courcet	courcet	PROPN
bionys-354	193	10	,	,	PUNCT
bionys-354	193	11	e.	e.	PROPN
bionys-354	193	12	,	,	PUNCT
bionys-354	193	13	martin	martin	PROPN
bionys-354	193	14	,	,	PUNCT
bionys-354	193	15	l.	l.	PROPN
bionys-354	193	16	,	,	PUNCT
bionys-354	193	17	lepage	lepage	PROPN
bionys-354	193	18	,	,	PUNCT
bionys-354	193	19	c.	c.	NOUN
bionys-354	193	20	,	,	PUNCT
bionys-354	193	21	faivre	faivre	PROPN
bionys-354	193	22	,	,	PUNCT
bionys-354	193	23	j.	j.	PROPN
bionys-354	193	24	,	,	PUNCT
bionys-354	193	25	piard	piard	NOUN
bionys-354	193	26	,	,	PUNCT
bionys-354	193	27	f.	f.	PROPN
bionys-354	193	28	2012	2012	NUM
bionys-354	193	29	:	:	PUNCT
bionys-354	193	30	why	why	SCONJ
bionys-354	193	31	do	do	AUX
bionys-354	193	32	results	result	VERB
bionys-354	193	33	conflict	conflict	NOUN
bionys-354	193	34	regarding	regard	VERB
bionys-354	193	35	the	the	DET
bionys-354	193	36	prognostic	prognostic	ADJ
bionys-354	193	37	value	value	NOUN
bionys-354	193	38	of	of	ADP
bionys-354	193	39	the	the	DET
bionys-354	193	40	methylation	methylation	NOUN
bionys-354	193	41	status	status	NOUN
bionys-354	193	42	in	in	ADP
bionys-354	193	43	colon	colon	NOUN
bionys-354	193	44	cancers	cancer	NOUN
bionys-354	193	45	?	?	PUNCT
bionys-354	194	1	the	the	DET
bionys-354	194	2	role	role	NOUN
bionys-354	194	3	of	of	ADP
bionys-354	194	4	the	the	DET
bionys-354	194	5	preservation	preservation	NOUN
bionys-354	194	6	method	method	NOUN
bionys-354	194	7	.	.	PUNCT
bionys-354	195	1	bmc	bmc	NOUN
bionys-354	195	2	cancer	cancer	NOUN
bionys-354	195	3	,	,	PUNCT
bionys-354	195	4	12	12	NUM
bionys-354	195	5	:	:	SYM
bionys-354	195	6	12	12	NUM
bionys-354	195	7	.	.	PUNCT
bionys-354	196	1	verhaak	verhaak	NOUN
bionys-354	196	2	,	,	PUNCT
bionys-354	196	3	r.g	r.g	PROPN
bionys-354	196	4	.	.	PROPN
bionys-354	196	5	,	,	PUNCT
bionys-354	196	6	hoadley	hoadley	PROPN
bionys-354	196	7	,	,	PUNCT
bionys-354	196	8	k.a	k.a	PROPN
bionys-354	196	9	.	.	PROPN
bionys-354	196	10	,	,	PUNCT
bionys-354	196	11	purdom	purdom	NOUN
bionys-354	196	12	,	,	PUNCT
bionys-354	196	13	e.	e.	PROPN
bionys-354	196	14	,	,	PUNCT
bionys-354	196	15	wang	wang	PROPN
bionys-354	196	16	,	,	PUNCT
bionys-354	196	17	v.	v.	PROPN
bionys-354	196	18	,	,	PUNCT
bionys-354	196	19	qi	qi	PROPN
bionys-354	196	20	,	,	PUNCT
bionys-354	196	21	y.	y.	PROPN
bionys-354	196	22	,	,	PUNCT
bionys-354	196	23	wilkerson	wilkerson	PROPN
bionys-354	196	24	,	,	PUNCT
bionys-354	196	25	m.d	m.d	PROPN
bionys-354	196	26	.	.	PROPN
bionys-354	196	27	,	,	PUNCT
bionys-354	196	28	miller	miller	PROPN
bionys-354	196	29	,	,	PUNCT
bionys-354	196	30	c.d	c.d	PROPN
bionys-354	196	31	.	.	PROPN
bionys-354	196	32	,	,	PUNCT
bionys-354	196	33	ding	ding	PROPN
bionys-354	196	34	,	,	PUNCT
bionys-354	196	35	l.	l.	PROPN
bionys-354	196	36	,	,	PUNCT
bionys-354	196	37	golub	golub	PROPN
bionys-354	196	38	,	,	PUNCT
bionys-354	196	39	t.	t.	PROPN
bionys-354	196	40	,	,	PUNCT
bionys-354	196	41	mesirov	mesirov	PROPN
bionys-354	196	42	,	,	PUNCT
bionys-354	196	43	j.p	j.p	PROPN
bionys-354	196	44	.	.	PROPN
bionys-354	196	45	,	,	PUNCT
bionys-354	196	46	alexe	alexe	PROPN
bionys-354	196	47	,	,	PUNCT
bionys-354	196	48	g.	g.	PROPN
bionys-354	196	49	,	,	PUNCT
bionys-354	196	50	lawrence	lawrence	PROPN
bionys-354	196	51	,	,	PUNCT
bionys-354	196	52	m	m	PROPN
bionys-354	196	53	,	,	PUNCT
bionys-354	196	54	o’kelly	o’kelly	ADV
bionys-354	196	55	,	,	PUNCT
bionys-354	196	56	m.	m.	NOUN
bionys-354	196	57	,temayo	,temayo	PUNCT
bionys-354	196	58	,	,	PUNCT
bionys-354	196	59	p.	p.	NOUN
bionys-354	196	60	,	,	PUNCT
bionys-354	196	61	weir	weir	PROPN
bionys-354	196	62	,	,	PUNCT
bionys-354	196	63	b.a	b.a	PROPN
bionys-354	196	64	.	.	PROPN
bionys-354	196	65	,	,	PUNCT
bionys-354	196	66	gabriel	gabriel	PROPN
bionys-354	196	67	,	,	PUNCT
bionys-354	196	68	s.	s.	PROPN
bionys-354	196	69	,	,	PUNCT
bionys-354	196	70	winckler	winckler	NOUN
bionys-354	196	71	,	,	PUNCT
bionys-354	196	72	w.	w.	PROPN
bionys-354	196	73	,	,	PUNCT
bionys-354	196	74	gupta	gupta	PROPN
bionys-354	196	75	,	,	PUNCT
bionys-354	196	76	s.	s.	PROPN
bionys-354	196	77	,	,	PUNCT
bionys-354	196	78	jakkula	jakkula	PROPN
bionys-354	196	79	,	,	PUNCT
bionys-354	196	80	l.	l.	PROPN
bionys-354	196	81	,	,	PUNCT
bionys-354	196	82	feiler	feiler	NOUN
bionys-354	196	83	,	,	PUNCT
bionys-354	196	84	h.s	h.s	PROPN
bionys-354	196	85	.	.	PROPN
bionys-354	196	86	,	,	PUNCT
bionys-354	196	87	hodgson	hodgson	PROPN
bionys-354	196	88	,	,	PUNCT
bionys-354	196	89	j.g	j.g	PROPN
bionys-354	196	90	.	.	PROPN
bionys-354	196	91	,	,	PUNCT
bionys-354	196	92	james	james	PROPN
bionys-354	196	93	,	,	PUNCT
bionys-354	196	94	c.d	c.d	PROPN
bionys-354	196	95	.	.	PROPN
bionys-354	196	96	,	,	PUNCT
bionys-354	196	97	sarkaria	sarkaria	PROPN
bionys-354	196	98	,	,	PUNCT
bionys-354	196	99	j.n	j.n	PROPN
bionys-354	196	100	.	.	PROPN
bionys-354	196	101	,	,	PUNCT
bionys-354	196	102	brennan	brennan	PROPN
bionys-354	196	103	,	,	PUNCT
bionys-354	196	104	c.	c.	PROPN
bionys-354	196	105	,	,	PUNCT
bionys-354	196	106	kahn	kahn	PROPN
bionys-354	196	107	,	,	PUNCT
bionys-354	196	108	a.	a.	PROPN
bionys-354	196	109	,	,	PUNCT
bionys-354	196	110	spellman	spellman	PROPN
bionys-354	196	111	,	,	PUNCT
bionys-354	196	112	p.t	p.t	PROPN
bionys-354	196	113	.	.	PROPN
bionys-354	196	114	,	,	PUNCT
bionys-354	196	115	wilson	wilson	PROPN
bionys-354	196	116	,	,	PUNCT
bionys-354	196	117	r.k	r.k	PROPN
bionys-354	196	118	.	.	PROPN
bionys-354	196	119	,	,	PUNCT
bionys-354	196	120	speed	speed	NOUN
bionys-354	196	121	,	,	PUNCT
bionys-354	196	122	t.p	t.p	PROPN
bionys-354	196	123	.	.	PROPN
bionys-354	196	124	,	,	PUNCT
bionys-354	196	125	gray	gray	ADJ
bionys-354	196	126	,	,	PUNCT
bionys-354	196	127	j.w	j.w	PROPN
bionys-354	196	128	.	.	PROPN
bionys-354	196	129	,	,	PUNCT
bionys-354	196	130	meyerson	meyerson	PROPN
bionys-354	196	131	,	,	PUNCT
bionys-354	196	132	m.	m.	NOUN
bionys-354	196	133	,	,	PUNCT
bionys-354	196	134	getz	getz	NOUN
bionys-354	196	135	,	,	PUNCT
bionys-354	196	136	g.	g.	PROPN
bionys-354	196	137	,	,	PUNCT
bionys-354	196	138	perou	perou	NOUN
bionys-354	196	139	,	,	PUNCT
bionys-354	196	140	c.m	c.m	PROPN
bionys-354	196	141	.	.	PROPN
bionys-354	196	142	,	,	PUNCT
bionys-354	196	143	hayes	hayes	PROPN
bionys-354	196	144	,	,	PUNCT
bionys-354	196	145	dn	dn	PROPN
bionys-354	196	146	.	.	PROPN
bionys-354	196	147	;	;	PUNCT
bionys-354	196	148	cancer	cancer	NOUN
bionys-354	196	149	genome	genome	NOUN
bionys-354	196	150	atlas	atlas	PROPN
bionys-354	196	151	research	research	PROPN
bionys-354	196	152	network	network	NOUN
bionys-354	196	153	.	.	PUNCT
bionys-354	197	1	2010	2010	NUM
bionys-354	197	2	:	:	PUNCT
bionys-354	197	3	integrated	integrate	VERB
bionys-354	197	4	genomic	genomic	NOUN
bionys-354	197	5	analysis	analysis	NOUN
bionys-354	197	6	identifies	identify	VERB
bionys-354	197	7	clinically	clinically	ADV
bionys-354	197	8	relevant	relevant	ADJ
bionys-354	197	9	subtypes	subtype	NOUN
bionys-354	197	10	of	of	ADP
bionys-354	197	11	glioblastoma	glioblastoma	NOUN
bionys-354	197	12	characterized	characterize	VERB
bionys-354	197	13	by	by	ADP
bionys-354	197	14	abnormalities	abnormality	NOUN
bionys-354	197	15	in	in	ADP
bionys-354	197	16	pdgfra	pdgfra	NOUN
bionys-354	197	17	,	,	PUNCT
bionys-354	197	18	idh1	idh1	PROPN
bionys-354	197	19	,	,	PUNCT
bionys-354	197	20	egfr	egfr	NOUN
bionys-354	197	21	,	,	PUNCT
bionys-354	197	22	and	and	CCONJ
bionys-354	197	23	nf1	nf1	NOUN
bionys-354	197	24	.	.	PUNCT
bionys-354	198	1	cancer	cancer	NOUN
bionys-354	198	2	cell	cell	NOUN
bionys-354	198	3	,	,	PUNCT
bionys-354	198	4	17(1	17(1	NUM
bionys-354	198	5	):	):	PUNCT
bionys-354	198	6	98	98	NUM
bionys-354	198	7	-	-	SYM
bionys-354	198	8	110	110	NUM
bionys-354	198	9	.	.	PUNCT
bionys-354	199	1	147	147	NUM