Biology, Medicine, & Natural Product Chemistry ISSN 2089-6514 (paper) Volume 10, Number 2, October 2021 | Pages: 117-121 | DOI: 10.14421/biomedich.2021.102.117-121 ISSN 2540-9328 (online) Antibacterial Activity of Mangrove Root Extracts from Ngurah Rai Mangrove Forest, Denpasar-Bali Made Dharmesti Wijaya1*, Anak Agung Gede Indraningrat2 1Pharmacology and Pharmacy Department; 2Microbiology and Parasitology Department, Faculty of Medicine and Health Sciences, Warmadewa University Jl. Terompong No 24 Denpasar 80235, Tel. +62 361 240727, Indonesia. Corresponding author* dharmestiwijaya@gmail.com Manuscript received: 08 October 2021. Revision accepted: 15 October, 2021. Published: 21 October, 2021. Abstract The increasing rate of antimicrobial resistance in the past decades has motivated the search for novel antibacterial compounds to overcome infectious diseases. Among diverse natural sources, mangrove ecosystems offer untapped sources of biological active compounds for future antibacterial medicine. This research was aimed to evaluate antibacterial activities of crude extracts of four dominant mangrove plants from the Ngurah Rai Mangrove Forest namely Rhizophora mucronata, Avicennia marina, Rhizophora apiculata, and Sonneratia alba. Roots of these four plants were extracted using methanol, chloroform, and n-hexane. These crude extracts were tested against two Gram positive bacteria (Staphylococcus aureus and Streptococcus mutans) and two Gram negative bacteria (Escherichia coli dan Klebsiella pneumoniae) by disc difussion assay. We found that 3 mg/mL of N-hexane crude extracts from R. apiculata yielded the highest zone of inhibition of 8.64 mm against S. aureus. While, 3 mg/mL of chloroform crude extract of R. apiculata yielded the highest inhibition of 19.83 mm against S. mutans. Unfortunately, no zone of inhibition was observed when crude extracts were tested against Gram negative indicator strains. Our results indicate that the root crude extracts of R. apiculata yielded the highest zone of inhibition against Gram positive indicator strains compared to root crude extracts of R. mucronata, S. alba, and A. marina. Further research is required to determine the antibacterial activities of the mangrove crude extracts against other bacterial indicator strains to determine their spectrum of activities. Keywords: mangrove; roots, antibacterial; Rhizopora apiculata. INTRODUCTION Antimicrobial substances play a crucial role during prevention and medication of infectious diseases, post- operation infections, infections after chemotherapy or infections for diabetic patients, late stage of kidney diseases and patients with rheumatoid arthritis (Ventola, 2015). However, incidence rate of antimicrobial resistance has increased dramatically in the past decades (Ventola, 2015), which cause difficulty to treat infectious diseases, increase cost for medication, and lead to higher mortality rate (World Health Organization, 2020). World Health Organization reported approximately 700,000 mortality every year until 2014 due to antimicrobial resistance and it is predicted the casualties of antimicrobial resistance reached 10 million in 2050 (Departemen Kesehatan Republik Indonesia, 2016). Immediate actions are required to overcome the increasing rate of antimicrobial resistance such as a better diagnose and prescription of antibiotics, optimization of therapy regimen, and prevention of infectious diseases (Ventola, 2015). In addition, the search for novel antimicrobial compounds are urgently needed since many antibiotics on the market are no longer effective to eradicate bacterial infections (Ventola, 2015). Among natural resources, mangrove ecosystems are the ideal habitat to search for novel antimicrobial activities. Mangrove refers to ecosystem where seawater and fresh water dissolve, which characterize by plants that can tolerate high salinity and limited oxygen (Friess, 2016). A number of studies showed that mangrove plants are rich of various of active compounds such as alkaloid, steroid, terpenoid, saponin, tannin, flavonoid dan poliphenol (Dahibhate et al., 2018; Gouda et al., 2015; Patra & Mohanta, 2014). Furthermore, diversity of these secondary metabolites led to broad biological activities such as antibacterial, antifungal, antioxidant, antiviral, and anticancer, which overall could potentially be developed as the source of new drugs (Patra & Thatoi, 2011; Saranraj, 2015). A study conducted in Kalimantan showed antibacterial activities of root of mangrove plant of Rhizophora apiculata (Usman, 2018). However, a similar study from mangrove plants found in Bali have not been conducted. The Ngurah Rai Mangrove Forest is the biggest mangrove ecosystem in Bali which has more than 16 different mangrove spesies and the four https://doi.org/10.14421/biomedich.2021.102.117-121 118 Biology, Medicine, & Natural Product Chemistry 10 (2), 2021: 117-121 dominant species are Rhizophora mucronata, Avicennia marina, Rhizophora apiculata and Sonneratia alba (Lugina et al., 2017). To date, antibacterial properties of these four dominant mangrove species are rather unexplored. Therefore, we aimed to screen for antibacterial activities of crude extract of the root of these four mangrove species by using three different solvents: methanol, chloroform and n-hexane against Gram negative and positive indicator strains. It is expected antibacterial activities of these crude extracts will shed light to explore antibacterial active compounds from root of mangrove plants. MATERIALS AND METHODS Materials Roots of the four dominant mangrove plants: Rhizophora mucronata, Avicennia marina, Rhizophora apiculata and Sonneratia alba were collected from the Ngurah Rai Mangrove Forest (8o43’40.4886” S, 115o11’42.80313” E), Bali, Indonesia, on August 2019 during the low tide. The species determination was carried out in Eka Karya Botanical Garden, Bedugul, Tabanan, Bali, Indonesia. The solvents (methanol, chloroform, and n-hexane) were purchased from Merck, Germany. Methods Sample extraction For each of mangrove species, 50 g of each dry powder of mangrove root were macerated twice using 250 mL of individual solvent (methanol, chloroform, or n-hexane) at room temperature for 24 hours and filtered using Whatmann filter paper No. 1. The extracts were then evaporated using rotary evaporator (IKA®, RV8) at 50°C, 90 rpm and stored in closed and air tight vial at 4°C until further used. Antibacterial screening From each mangrove root extract, three level of concentrations were prepared: 1 mg/mL, 3 mg/mL and 5 mg/mL. Subsequently, each crude extract was exposed against bacterial indicator strains Gram positive (Staphylococcus aureus dan Streptococcus mutans) and Gram negative (Escherichia coli dan Klebsiella pneumoniae) by disc diffusion assay. One hundred microlitre of bacterial indicator strains were spotted on Luria Bertani agar and spread using a sterile spreader. Thirty microlitre of crude extracts were spotted on a 6 mm paper disc and air dried for 30 minutes. Paper discs in triplicates containing crude extracts were placed on agar media containing bacterial indicator strains. Ampicillin and Streptomycin were used as positive controls, while the solvents, methanol, chloroform, and n-hexane were used as negative controls. Antibacterial activities were calculated based on average zone of inhibition (ZOI) that were formed from triplicate disc. RESULTS AND DISCUSSION The results showed that antibacterial activities were displayed by some extracts against Gram positive strains, S. aureus and S. Mutans (Table 1), but none against Gram negative strains, E. coli dan K. pneumoniae. Among the four mangrove species, only R. apiculata exhibited antibacterial properties against both Gram positive indicator strains. The concentration of 3 mg/ml N-hexane extract showed the highest ZOI of 8.64 ± 0.13 mm against S. aureus while 3 mg/ml chloroform extract yielded the highest ZOI of 19.83 ± 0.63 mm against S. mutans. A study by Seepana and co-workers (2016) also showed that methanol, ethanol, and hexane extract of R. apiculata roots is active against Salmonela typhi and S. aureus. Their study also showed that methanol, hexane, and ethanol R. apiculata root extracts have no activity against E. coli. However, there are activities against other Gram-negative bacteria Salmonella typhi, shown by methanol, hexane, and ethanol extracts of R. apiculata root. Ethanolic extract of R. apiculata root also displays activity against K. pneumonia (Seepana et al. 2016). Figure 1. Antimicrobial activity of R. apiculata N-hexane extracts in triplicates against S. aureus. The concentration of 3 mg/ml N-hexane extract showed the highest ZOI (8.64 ± 0.13 mm). 5 mg/ml 5 mg/ml 5 mg/ml 3 mg/ml 3 mg/ml 1 mg/ml 1 mg/ml 3 mg/ml 1 mg/ml Wijaya & Indraningrat – Antibacterial Activity of Mangrove Root 119 Figure 2. Antimicrobial activity of R. apiculata chloroform extracts in triplicates against S. mutans. The concentration of 3 mg/ml chloroform extract yielded the highest ZOI of 19.83 ± 0.63 mm against S. mutans. Table 1. Antibacterial activities of mangrove root extracts. Bacterial Strains Samples Solvents Concentration ZOI (mm) Staphylococcus aureus Rhizopora apiculata Methanol 1 mg/ml - 3 mg/ml - 5 mg/ml - Chloroform 1 mg/ml - 3 mg/ml - 5 mg/ml - N-hexane 1 mg/ml 8.05 ± 0.64 3 mg/ml 8.64 ± 0.13 5 mg/ml 7.84 ± 0.61 Rhizopora mucronata Methanol 1 mg/ml - 3 mg/ml - 5 mg/ml - Chloroform 1 mg/ml - 3 mg/ml - 5 mg/ml - N-hexane 1 mg/ml - 3 mg/ml - 5 mg/ml - Sonneratia alba Methanol 1 mg/ml - 3 mg/ml - 5 mg/ml - Chloroform 1 mg/ml - 3 mg/ml - 5 mg/ml - N-hexane 1 mg/ml - 3 mg/ml - 5 mg/ml - Avicennia marina Methanol 1 mg/ml - 3 mg/ml - 5 mg/ml - Chloroform 1 mg/ml - 3 mg/ml - 5 mg/ml - N-hexane 1 mg/ml - 3 mg/ml - 5 mg/ml - Positive controls Ampicillin 10 μg 19.20 ± 0.98 Streptomycin 10 μg 15.54 ± 0.22 Negative controls Methanol - - Chloroform - - N-hexane - - 3 mg/ml 3 mg/ml 3 mg/ml 1 mg/ml 1 mg/ml 5 mg/ml 1 mg/ml 5 mg/ml 5 mg/ml 120 Biology, Medicine, & Natural Product Chemistry 10 (2), 2021: 117-121 Bacterial Strains Samples Solvents Concentration ZOI (mm) Streptococcus mutans Rhizopora apiculata Methanol 1 mg/ml - 3 mg/ml - 5 mg/ml - Chloroform 1 mg/ml 13.98 ± 1.49 3 mg/ml 19.83 ± 0.63 5 mg/ml 15.92 ± 0.68 N-hexane 1 mg/ml - 3 mg/ml - 5 mg/ml - Rhizopora mucronata Methanol 1 mg/ml - 3 mg/ml - 5 mg/ml - Chloroform 1 mg/ml - 3 mg/ml - 5 mg/ml - N-hexane 1 mg/ml - 3 mg/ml - 5 mg/ml 7.32 ± 0.43 Sonneratia alba Methanol 1 mg/ml - 3 mg/ml - 5 mg/ml - Chloroform 1 mg/ml - 3 mg/ml - 5 mg/ml - N-hexane 1 mg/ml - 3 mg/ml 7.39 ± 0.21 5 mg/ml 7.96 ± 0.25 Avicennia marina Methanol 1 mg/ml - 3 mg/ml - 5 mg/ml - Chloroform 1 mg/ml - 3 mg/ml - 5 mg/ml - N-hexane 1 mg/ml - 3 mg/ml - 5 mg/ml - Positive controls Ampicillin 10 μg 31.72 ± 3.03 Streptomycin 10 μg 20.76 ± 1.49 Negative controls Methanol - - Chloroform - - N-hexane - - In addition, other Rhizopora species namely R. mucronata also displayed antibacterial activity in which 5 mg/ml n-hexane extract showed ZOI of 7.32 ± 0.43 mm against S. mutans. Rhizopora species including R. apiculata and R. mucronata contain many medically important secondary metabolites such as essential oils, glycosides, alkaloids, phenolic compounds, and tannins (Habib et al., 2018). Tannins is known to have antimicrobial properties against both Gram-positive and Gram-negative bacteria. This compound passes the bacterial cell wall and intrudes the cell metabolism, resulted in bacterial death (Kaczmarek, 2020). Furthermore, N-hexane extract of S. alba root showed activity against S. mutans in which the concentration of 3 mg/ml and 5 mg/ml yielded ZOI of 7.39 ± 0.21 and 7.96 ± 0.25 mm respectively. Although study on S. alba root extract is still limited, there are a number of studies focusing on leaves and bark of this plant. A study by Saad and co-workers (2012) showed that methanolic extract of S. alba leaves antibacterial activities were observed against Gram-negative bacteria E. coli (17,5 mm), as well as Gram-positive bacteria namely S. aureus (12.5 mm) and Bacillus cereus (12.5 mm) (Saad et al., 2012). Our result indicate that overall, crude extract of mangrove root displayed much lower antibacterial activities compared to positive control ampicillin (10 μg) dan streptomycin (10 μg). However, zone of inhibition R. apiculata crude extracts using n-hexane (3 mg/L) were comparable to that of streptomycin. Therefore, R. apiculata could potentially contain novel antibacterial compounds. However, composition of active compounds in the crude extracts need to be confirmed. It is expected that once this active compound Wijaya & Indraningrat – Antibacterial Activity of Mangrove Root 121 has been identified, it can be isolated and further developed as new antibiotics drugs. CONCLUSION In conclusion, crude extracts of root R. apiculata displayed the highest antibacterial activities compared to other crude extracts of different mangrove plants. We found that extraction using n-hexane yielded the highest inhibition zones compared to methanol and chloroform. Further research is required to elucidate the active compounds in n-hexane. Acknowledgements: We would like to acknowledged financial support from UP2M of Faculty of Medicines and Health Sciences under grant no. 417/Unwar/FKIK/UP2M/KP-02/VII/2019. Conflicts of Interest: The authors declare that there are no conflicts of interest. REFERENCES Dahibhate, N., Saddhe, A., & Kumar, K. (2018). Mangrove plants as a source of bioactive compounds: A review. 08. doi:10.2174/2210315508666180910125328 Departemen Kesehatan Republik Indonesia. (2016). Mari Bersama Atasi Resistensi Antimikroba (AMR). Friess, D. A. (2016). Mangrove Forests. Current Biology Magazine, 26, R746-R748. Gouda, S., Das, G., & Patra, J. K. 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