id	sid	tid	token	lemma	pos
brj-24573	1	1	peer	peer	NOUN
brj-24573	1	2	-	-	PUNCT
brj-24573	1	3	review	review	NOUN
brj-24573	1	4	article	article	NOUN
brj-24573	1	5	peer	peer	NOUN
brj-24573	1	6	-	-	PUNCT
brj-24573	1	7	reviewed	review	VERB
brj-24573	1	8	article	article	NOUN
brj-24573	1	9	bioresources.cnr.ncsu.edu	bioresources.cnr.ncsu.edu	X
brj-24573	1	10	farouk	farouk	PROPN
brj-24573	1	11	et	et	PROPN
brj-24573	1	12	al	al	PROPN
brj-24573	1	13	.	.	PROPN
brj-24573	2	1	(	(	PUNCT
brj-24573	2	2	2025	2025	NUM
brj-24573	2	3	)	)	PUNCT
brj-24573	2	4	.	.	PUNCT
brj-24573	3	1	“	"	PUNCT
brj-24573	3	2	amylase	amylase	NOUN
brj-24573	3	3	via	via	ADP
brj-24573	3	4	xylose	xylose	PROPN
brj-24573	3	5	promoter	promoter	NOUN
brj-24573	3	6	,	,	PUNCT
brj-24573	3	7	”	"	PUNCT
brj-24573	3	8	bioresources	bioresource	NOUN
brj-24573	3	9	20(3	20(3	NOUN
brj-24573	3	10	)	)	PUNCT
brj-24573	3	11	,	,	PUNCT
brj-24573	3	12	7728	7728	NUM
brj-24573	3	13	-	-	SYM
brj-24573	3	14	7737	7737	NUM
brj-24573	3	15	.	.	PUNCT
brj-24573	4	1	7728	7728	NUM
brj-24573	4	2	efficient	efficient	ADJ
brj-24573	4	3	extracellular	extracellular	ADJ
brj-24573	4	4	production	production	NOUN
brj-24573	4	5	of	of	ADP
brj-24573	4	6	α	α	NOUN
brj-24573	4	7	-	-	NOUN
brj-24573	4	8	amylase	amylase	NOUN
brj-24573	4	9	in	in	ADP
brj-24573	4	10	bacillus	bacillus	NOUN
brj-24573	4	11	subtilis	subtili	NOUN
brj-24573	4	12	under	under	ADP
brj-24573	4	13	the	the	DET
brj-24573	4	14	influence	influence	NOUN
brj-24573	4	15	of	of	ADP
brj-24573	4	16	xylose	xylose	PROPN
brj-24573	4	17	-	-	PUNCT
brj-24573	4	18	inducible	inducible	ADJ
brj-24573	4	19	promoter	promoter	NOUN
brj-24573	4	20	abd	abd	PROPN
brj-24573	4	21	-	-	PROPN
brj-24573	4	22	el	el	PROPN
brj-24573	4	23	aziem	aziem	PROPN
brj-24573	4	24	farouk	farouk	PROPN
brj-24573	4	25	,	,	PUNCT
brj-24573	4	26	a	a	DET
brj-24573	4	27	,	,	PUNCT
brj-24573	4	28	*	*	PUNCT
brj-24573	4	29	iqra	iqra	NOUN
brj-24573	4	30	aroob	aroob	NOUN
brj-24573	4	31	,	,	PUNCT
brj-24573	4	32	b	b	X
brj-24573	4	33	quratulain	quratulain	NOUN
brj-24573	4	34	amjad	amjad	NOUN
brj-24573	4	35	,	,	PUNCT
brj-24573	4	36	c	c	PROPN
brj-24573	4	37	hala	hala	PROPN
brj-24573	4	38	mohamed	mohamed	PROPN
brj-24573	4	39	abdelmigid	abdelmigid	PROPN
brj-24573	4	40	,	,	PUNCT
brj-24573	4	41	a	a	PRON
brj-24573	4	42	and	and	CCONJ
brj-24573	4	43	ralf	ralf	PROPN
brj-24573	4	44	greiner	greiner	PROPN
brj-24573	4	45	,	,	PUNCT
brj-24573	5	1	d	d	PROPN
brj-24573	5	2	this	this	DET
brj-24573	5	3	study	study	NOUN
brj-24573	5	4	focuses	focus	VERB
brj-24573	5	5	on	on	ADP
brj-24573	5	6	the	the	DET
brj-24573	5	7	development	development	NOUN
brj-24573	5	8	of	of	ADP
brj-24573	5	9	a	a	DET
brj-24573	5	10	xylose	xylose	PROPN
brj-24573	5	11	-	-	PUNCT
brj-24573	5	12	inducible	inducible	ADJ
brj-24573	5	13	system	system	NOUN
brj-24573	5	14	to	to	PART
brj-24573	5	15	produce	produce	VERB
brj-24573	5	16	recombinant	recombinant	ADJ
brj-24573	5	17	α	α	NOUN
brj-24573	5	18	-	-	NOUN
brj-24573	5	19	amylase	amylase	NOUN
brj-24573	5	20	from	from	ADP
brj-24573	5	21	the	the	DET
brj-24573	5	22	bacterium	bacterium	NOUN
brj-24573	5	23	thermoactinomyces	thermoactinomyce	NOUN
brj-24573	5	24	vulgaris	vulgaris	NOUN
brj-24573	5	25	94	94	NUM
brj-24573	5	26	-	-	SYM
brj-24573	5	27	2a	2a	NUM
brj-24573	5	28	(	(	PUNCT
brj-24573	5	29	amytv	amytv	NOUN
brj-24573	5	30	)	)	PUNCT
brj-24573	5	31	in	in	ADP
brj-24573	5	32	the	the	DET
brj-24573	5	33	bacillus	bacillus	NOUN
brj-24573	5	34	expression	expression	NOUN
brj-24573	5	35	system	system	NOUN
brj-24573	5	36	.	.	PUNCT
brj-24573	6	1	to	to	PART
brj-24573	6	2	achieve	achieve	VERB
brj-24573	6	3	this	this	PRON
brj-24573	6	4	,	,	PUNCT
brj-24573	6	5	a	a	DET
brj-24573	6	6	gene	gene	NOUN
brj-24573	6	7	cassette	cassette	NOUN
brj-24573	6	8	was	be	AUX
brj-24573	6	9	constructed	construct	VERB
brj-24573	6	10	using	use	VERB
brj-24573	6	11	pwh1520	pwh1520	PROPN
brj-24573	6	12	shuttle	shuttle	PROPN
brj-24573	6	13	vector	vector	PROPN
brj-24573	6	14	,	,	PUNCT
brj-24573	6	15	containing	contain	VERB
brj-24573	6	16	a	a	DET
brj-24573	6	17	pxyla	pxyla	NOUN
brj-24573	6	18	promoter	promoter	NOUN
brj-24573	6	19	comprising	comprising	NOUN
brj-24573	6	20	glucose	glucose	NOUN
brj-24573	6	21	box	box	NOUN
brj-24573	6	22	and	and	CCONJ
brj-24573	6	23	amytv	amytv	NOUN
brj-24573	6	24	gene	gene	NOUN
brj-24573	6	25	.	.	PUNCT
brj-24573	7	1	the	the	DET
brj-24573	7	2	cassette	cassette	NOUN
brj-24573	7	3	was	be	AUX
brj-24573	7	4	initially	initially	ADV
brj-24573	7	5	cloned	clone	VERB
brj-24573	7	6	in	in	ADP
brj-24573	7	7	escherichia	escherichia	NOUN
brj-24573	7	8	coli	coli	NOUN
brj-24573	7	9	for	for	ADP
brj-24573	7	10	replication	replication	NOUN
brj-24573	7	11	and	and	CCONJ
brj-24573	7	12	subsequently	subsequently	ADV
brj-24573	7	13	,	,	PUNCT
brj-24573	7	14	introduced	introduce	VERB
brj-24573	7	15	into	into	ADP
brj-24573	7	16	the	the	DET
brj-24573	7	17	bacillus	bacillus	NOUN
brj-24573	7	18	subtilis	subtilis	NOUN
brj-24573	7	19	(	(	PUNCT
brj-24573	7	20	gsb26	gsb26	NOUN
brj-24573	7	21	)	)	PUNCT
brj-24573	7	22	for	for	ADP
brj-24573	7	23	expression	expression	NOUN
brj-24573	7	24	.	.	PUNCT
brj-24573	8	1	to	to	PART
brj-24573	8	2	optimize	optimize	VERB
brj-24573	8	3	the	the	DET
brj-24573	8	4	secretion	secretion	NOUN
brj-24573	8	5	of	of	ADP
brj-24573	8	6	α	α	NOUN
brj-24573	8	7	-	-	NOUN
brj-24573	8	8	amylase	amylase	NOUN
brj-24573	8	9	,	,	PUNCT
brj-24573	8	10	different	different	ADJ
brj-24573	8	11	concentrations	concentration	NOUN
brj-24573	8	12	of	of	ADP
brj-24573	8	13	xylose	xylose	PROPN
brj-24573	8	14	were	be	AUX
brj-24573	8	15	used	use	VERB
brj-24573	8	16	as	as	ADP
brj-24573	8	17	inducers	inducer	NOUN
brj-24573	8	18	.	.	PUNCT
brj-24573	9	1	it	it	PRON
brj-24573	9	2	was	be	AUX
brj-24573	9	3	found	find	VERB
brj-24573	9	4	that	that	SCONJ
brj-24573	9	5	the	the	DET
brj-24573	9	6	maximum	maximum	ADJ
brj-24573	9	7	amylase	amylase	NOUN
brj-24573	9	8	activity	activity	NOUN
brj-24573	9	9	was	be	AUX
brj-24573	9	10	achieved	achieve	VERB
brj-24573	9	11	when	when	SCONJ
brj-24573	9	12	2	2	NUM
brj-24573	9	13	%	%	NOUN
brj-24573	9	14	xylose	xylose	PROPN
brj-24573	9	15	was	be	AUX
brj-24573	9	16	used	use	VERB
brj-24573	9	17	as	as	ADP
brj-24573	9	18	the	the	DET
brj-24573	9	19	inducer	inducer	NOUN
brj-24573	9	20	.	.	PUNCT
brj-24573	10	1	furthermore	furthermore	ADV
brj-24573	10	2	,	,	PUNCT
brj-24573	10	3	using	use	VERB
brj-24573	10	4	glucose	glucose	NOUN
brj-24573	10	5	alone	alone	ADJ
brj-24573	10	6	and	and	CCONJ
brj-24573	10	7	in	in	ADP
brj-24573	10	8	combination	combination	NOUN
brj-24573	10	9	with	with	ADP
brj-24573	10	10	xylose	xylose	PROPN
brj-24573	10	11	as	as	SCONJ
brj-24573	10	12	inducers	inducer	NOUN
brj-24573	10	13	indicated	indicate	VERB
brj-24573	10	14	that	that	SCONJ
brj-24573	10	15	glucose	glucose	NOUN
brj-24573	10	16	acted	act	VERB
brj-24573	10	17	as	as	ADP
brj-24573	10	18	a	a	DET
brj-24573	10	19	catabolic	catabolic	ADJ
brj-24573	10	20	repressor	repressor	NOUN
brj-24573	10	21	for	for	ADP
brj-24573	10	22	amytv	amytv	NOUN
brj-24573	10	23	expression	expression	NOUN
brj-24573	10	24	.	.	PUNCT
brj-24573	11	1	moreover	moreover	ADV
brj-24573	11	2	,	,	PUNCT
brj-24573	11	3	protein	protein	NOUN
brj-24573	11	4	was	be	AUX
brj-24573	11	5	efficiently	efficiently	ADV
brj-24573	11	6	secreted	secrete	VERB
brj-24573	11	7	and	and	CCONJ
brj-24573	11	8	did	do	AUX
brj-24573	11	9	not	not	PART
brj-24573	11	10	accumulate	accumulate	VERB
brj-24573	11	11	in	in	ADP
brj-24573	11	12	the	the	DET
brj-24573	11	13	cellular	cellular	ADJ
brj-24573	11	14	fractions	fraction	NOUN
brj-24573	11	15	,	,	PUNCT
brj-24573	11	16	even	even	ADV
brj-24573	11	17	at	at	ADP
brj-24573	11	18	high	high	ADJ
brj-24573	11	19	expression	expression	NOUN
brj-24573	11	20	levels	level	NOUN
brj-24573	11	21	.	.	PUNCT
brj-24573	12	1	doi	doi	NOUN
brj-24573	12	2	:	:	PUNCT
brj-24573	12	3	10.15376	10.15376	NUM
brj-24573	12	4	/	/	SYM
brj-24573	12	5	biores.20.3.7728	biores.20.3.7728	PROPN
brj-24573	12	6	-	-	PUNCT
brj-24573	12	7	7737	7737	NUM
brj-24573	12	8	keywords	keyword	NOUN
brj-24573	12	9	:	:	PUNCT
brj-24573	12	10	recombinant	recombinant	ADJ
brj-24573	12	11	amylase	amylase	NOUN
brj-24573	12	12	;	;	PUNCT
brj-24573	12	13	bacillus	bacillus	NOUN
brj-24573	12	14	expression	expression	NOUN
brj-24573	12	15	systems	system	NOUN
brj-24573	12	16	;	;	PUNCT
brj-24573	12	17	catabolic	catabolic	ADJ
brj-24573	12	18	repression	repression	NOUN
brj-24573	12	19	contact	contact	NOUN
brj-24573	12	20	information	information	NOUN
brj-24573	12	21	:	:	PUNCT
brj-24573	12	22	a	a	DET
brj-24573	12	23	:	:	PUNCT
brj-24573	12	24	department	department	NOUN
brj-24573	12	25	of	of	ADP
brj-24573	12	26	biotechnology	biotechnology	NOUN
brj-24573	12	27	,	,	PUNCT
brj-24573	12	28	college	college	NOUN
brj-24573	12	29	of	of	ADP
brj-24573	12	30	science	science	PROPN
brj-24573	12	31	,	,	PUNCT
brj-24573	12	32	taif	taif	PROPN
brj-24573	12	33	university	university	PROPN
brj-24573	12	34	,	,	PUNCT
brj-24573	12	35	p.o	p.o	PROPN
brj-24573	12	36	.	.	PROPN
brj-24573	12	37	box	box	PROPN
brj-24573	12	38	1109	1109	NUM
brj-24573	12	39	taif	taif	NOUN
brj-24573	12	40	21944	21944	NUM
brj-24573	12	41	,	,	PUNCT
brj-24573	12	42	saudi	saudi	PROPN
brj-24573	12	43	arabia	arabia	PROPN
brj-24573	12	44	;	;	PUNCT
brj-24573	12	45	b	b	X
brj-24573	12	46	:	:	PUNCT
brj-24573	12	47	school	school	NOUN
brj-24573	12	48	of	of	ADP
brj-24573	12	49	allied	ally	VERB
brj-24573	12	50	health	health	NOUN
brj-24573	12	51	sciences	sciences	PROPN
brj-24573	12	52	,	,	PUNCT
brj-24573	12	53	university	university	NOUN
brj-24573	12	54	of	of	ADP
brj-24573	12	55	child	child	PROPN
brj-24573	12	56	health	health	NOUN
brj-24573	12	57	sciences	science	NOUN
brj-24573	12	58	,	,	PUNCT
brj-24573	12	59	the	the	DET
brj-24573	12	60	children	child	NOUN
brj-24573	12	61	’s	’s	PART
brj-24573	12	62	hospital	hospital	NOUN
brj-24573	12	63	lahore	lahore	NOUN
brj-24573	12	64	;	;	PUNCT
brj-24573	13	1	c	c	X
brj-24573	13	2	:	:	PUNCT
brj-24573	13	3	school	school	NOUN
brj-24573	13	4	of	of	ADP
brj-24573	13	5	biological	biological	ADJ
brj-24573	13	6	sciences	science	NOUN
brj-24573	13	7	,	,	PUNCT
brj-24573	13	8	faculty	faculty	NOUN
brj-24573	13	9	of	of	ADP
brj-24573	13	10	allied	ally	VERB
brj-24573	13	11	health	health	NOUN
brj-24573	13	12	sciences	science	NOUN
brj-24573	13	13	,	,	PUNCT
brj-24573	13	14	superior	superior	ADJ
brj-24573	13	15	university	university	NOUN
brj-24573	13	16	,	,	PUNCT
brj-24573	13	17	raiwind	raiwind	NOUN
brj-24573	13	18	road	road	NOUN
brj-24573	13	19	,	,	PUNCT
brj-24573	13	20	lahore	lahore	NOUN
brj-24573	13	21	,	,	PUNCT
brj-24573	13	22	pakistan	pakistan	PROPN
brj-24573	13	23	;	;	PUNCT
brj-24573	13	24	d	d	X
brj-24573	13	25	:	:	PUNCT
brj-24573	13	26	department	department	NOUN
brj-24573	13	27	of	of	ADP
brj-24573	13	28	food	food	NOUN
brj-24573	13	29	technology	technology	NOUN
brj-24573	13	30	and	and	CCONJ
brj-24573	13	31	bioprocess	bioprocess	NOUN
brj-24573	13	32	engineering	engineering	NOUN
brj-24573	13	33	,	,	PUNCT
brj-24573	13	34	max	max	PROPN
brj-24573	13	35	rubner	rubner	PROPN
brj-24573	13	36	-	-	PUNCT
brj-24573	13	37	institut	institut	NOUN
brj-24573	13	38	,	,	PUNCT
brj-24573	13	39	federal	federal	PROPN
brj-24573	13	40	research	research	PROPN
brj-24573	13	41	institute	institute	PROPN
brj-24573	13	42	of	of	ADP
brj-24573	13	43	nutrition	nutrition	NOUN
brj-24573	13	44	and	and	CCONJ
brj-24573	13	45	food	food	NOUN
brj-24573	13	46	,	,	PUNCT
brj-24573	13	47	karlsruhe	karlsruhe	PROPN
brj-24573	13	48	,	,	PUNCT
brj-24573	13	49	germany	germany	PROPN
brj-24573	13	50	;	;	PUNCT
brj-24573	13	51	*	*	PUNCT
brj-24573	13	52	corresponding	correspond	VERB
brj-24573	13	53	author	author	NOUN
brj-24573	13	54	:	:	PUNCT
brj-24573	13	55	farouk@tu.edu.sa	farouk@tu.edu.sa	ADJ
brj-24573	13	56	introduction	introduction	NOUN
brj-24573	13	57	bacteria	bacteria	NOUN
brj-24573	13	58	have	have	AUX
brj-24573	13	59	emerged	emerge	VERB
brj-24573	13	60	as	as	ADP
brj-24573	13	61	valuable	valuable	ADJ
brj-24573	13	62	cell	cell	NOUN
brj-24573	13	63	factories	factory	NOUN
brj-24573	13	64	for	for	ADP
brj-24573	13	65	producing	produce	VERB
brj-24573	13	66	recombinant	recombinant	ADJ
brj-24573	13	67	proteins	protein	NOUN
brj-24573	13	68	,	,	PUNCT
brj-24573	13	69	offering	offer	VERB
brj-24573	13	70	a	a	DET
brj-24573	13	71	versatile	versatile	ADJ
brj-24573	13	72	platform	platform	NOUN
brj-24573	13	73	for	for	ADP
brj-24573	13	74	various	various	ADJ
brj-24573	13	75	biopolymer	biopolymer	NOUN
brj-24573	13	76	applications	application	NOUN
brj-24573	13	77	(	(	PUNCT
brj-24573	13	78	rygus	rygus	NOUN
brj-24573	13	79	and	and	CCONJ
brj-24573	13	80	hillen	hillen	ADJ
brj-24573	13	81	1992	1992	NUM
brj-24573	13	82	)	)	PUNCT
brj-24573	13	83	.	.	PUNCT
brj-24573	14	1	the	the	DET
brj-24573	14	2	production	production	NOUN
brj-24573	14	3	of	of	ADP
brj-24573	14	4	recombinant	recombinant	ADJ
brj-24573	14	5	enzymes	enzyme	NOUN
brj-24573	14	6	,	,	PUNCT
brj-24573	14	7	specifically	specifically	ADV
brj-24573	14	8	pure	pure	ADJ
brj-24573	14	9	amylases	amylase	NOUN
brj-24573	14	10	,	,	PUNCT
brj-24573	14	11	is	be	AUX
brj-24573	14	12	of	of	ADP
brj-24573	14	13	great	great	ADJ
brj-24573	14	14	significance	significance	NOUN
brj-24573	14	15	in	in	ADP
brj-24573	14	16	the	the	DET
brj-24573	14	17	biochemical	biochemical	ADJ
brj-24573	14	18	processing	processing	NOUN
brj-24573	14	19	of	of	ADP
brj-24573	14	20	starch	starch	NOUN
brj-24573	14	21	(	(	PUNCT
brj-24573	14	22	khlestkin	khlestkin	PROPN
brj-24573	14	23	et	et	PROPN
brj-24573	14	24	al	al	PROPN
brj-24573	14	25	.	.	PROPN
brj-24573	14	26	2018	2018	NUM
brj-24573	14	27	;	;	PUNCT
brj-24573	14	28	aroob	aroob	PROPN
brj-24573	14	29	et	et	PROPN
brj-24573	14	30	al	al	PROPN
brj-24573	14	31	.	.	PROPN
brj-24573	14	32	2021	2021	NUM
brj-24573	14	33	)	)	PUNCT
brj-24573	14	34	.	.	PUNCT
brj-24573	15	1	suitable	suitable	ADJ
brj-24573	15	2	host	host	NOUN
brj-24573	15	3	organisms	organism	NOUN
brj-24573	15	4	,	,	PUNCT
brj-24573	15	5	capable	capable	ADJ
brj-24573	15	6	of	of	ADP
brj-24573	15	7	producing	produce	VERB
brj-24573	15	8	recombinant	recombinant	ADJ
brj-24573	15	9	enzymes	enzyme	NOUN
brj-24573	15	10	in	in	ADP
brj-24573	15	11	substantial	substantial	ADJ
brj-24573	15	12	quantities	quantity	NOUN
brj-24573	15	13	,	,	PUNCT
brj-24573	15	14	are	be	AUX
brj-24573	15	15	crucial	crucial	ADJ
brj-24573	15	16	for	for	ADP
brj-24573	15	17	biochemical	biochemical	ADJ
brj-24573	15	18	engineering	engineering	NOUN
brj-24573	15	19	.	.	PUNCT
brj-24573	16	1	by	by	ADP
brj-24573	16	2	harnessing	harness	VERB
brj-24573	16	3	the	the	DET
brj-24573	16	4	potential	potential	NOUN
brj-24573	16	5	of	of	ADP
brj-24573	16	6	bacteria	bacteria	NOUN
brj-24573	16	7	as	as	ADP
brj-24573	16	8	efficient	efficient	ADJ
brj-24573	16	9	cell	cell	NOUN
brj-24573	16	10	factories	factory	NOUN
brj-24573	16	11	(	(	PUNCT
brj-24573	16	12	ferrer	ferrer	NOUN
brj-24573	16	13	-	-	NOUN
brj-24573	16	14	miralles	miralle	NOUN
brj-24573	16	15	and	and	CCONJ
brj-24573	16	16	villaverde	villaverde	ADJ
brj-24573	16	17	2013	2013	NUM
brj-24573	16	18	)	)	PUNCT
brj-24573	16	19	and	and	CCONJ
brj-24573	16	20	employing	employ	VERB
brj-24573	16	21	advanced	advanced	ADJ
brj-24573	16	22	cloning	cloning	NOUN
brj-24573	16	23	and	and	CCONJ
brj-24573	16	24	expression	expression	NOUN
brj-24573	16	25	techniques	technique	NOUN
brj-24573	16	26	,	,	PUNCT
brj-24573	16	27	researchers	researcher	NOUN
brj-24573	16	28	have	have	AUX
brj-24573	16	29	made	make	VERB
brj-24573	16	30	remarkable	remarkable	ADJ
brj-24573	16	31	strides	stride	NOUN
brj-24573	16	32	in	in	ADP
brj-24573	16	33	producing	produce	VERB
brj-24573	16	34	a	a	DET
brj-24573	16	35	wide	wide	ADJ
brj-24573	16	36	range	range	NOUN
brj-24573	16	37	of	of	ADP
brj-24573	16	38	recombinant	recombinant	ADJ
brj-24573	16	39	proteins	protein	NOUN
brj-24573	16	40	,	,	PUNCT
brj-24573	16	41	including	include	VERB
brj-24573	16	42	enzymes	enzyme	NOUN
brj-24573	16	43	,	,	PUNCT
brj-24573	16	44	with	with	ADP
brj-24573	16	45	various	various	ADJ
brj-24573	16	46	industrial	industrial	ADJ
brj-24573	16	47	,	,	PUNCT
brj-24573	16	48	medical	medical	ADJ
brj-24573	16	49	,	,	PUNCT
brj-24573	16	50	and	and	CCONJ
brj-24573	16	51	scientific	scientific	ADJ
brj-24573	16	52	applications	application	NOUN
brj-24573	16	53	(	(	PUNCT
brj-24573	16	54	terpe	terpe	NOUN
brj-24573	16	55	2006	2006	NUM
brj-24573	16	56	)	)	PUNCT
brj-24573	16	57	.	.	PUNCT
brj-24573	17	1	escherichia	escherichia	NOUN
brj-24573	17	2	coli	coli	NOUN
brj-24573	17	3	has	have	AUX
brj-24573	17	4	been	be	AUX
brj-24573	17	5	extensively	extensively	ADV
brj-24573	17	6	used	use	VERB
brj-24573	17	7	for	for	ADP
brj-24573	17	8	recombinant	recombinant	ADJ
brj-24573	17	9	protein	protein	NOUN
brj-24573	17	10	production	production	NOUN
brj-24573	17	11	(	(	PUNCT
brj-24573	17	12	tempe	tempe	PROPN
brj-24573	17	13	2006	2006	NUM
brj-24573	17	14	;	;	PUNCT
brj-24573	17	15	makino	makino	PROPN
brj-24573	17	16	et	et	PROPN
brj-24573	17	17	al	al	PROPN
brj-24573	17	18	.	.	PROPN
brj-24573	17	19	2011	2011	NUM
brj-24573	17	20	)	)	PUNCT
brj-24573	17	21	.	.	PUNCT
brj-24573	18	1	recently	recently	ADV
brj-24573	18	2	,	,	PUNCT
brj-24573	18	3	bacilli	bacilli	PROPN
brj-24573	18	4	have	have	AUX
brj-24573	18	5	garnered	garner	VERB
brj-24573	18	6	significant	significant	ADJ
brj-24573	18	7	attention	attention	NOUN
brj-24573	18	8	as	as	ADP
brj-24573	18	9	ideal	ideal	ADJ
brj-24573	18	10	host	host	NOUN
brj-24573	18	11	expression	expression	NOUN
brj-24573	18	12	systems	system	NOUN
brj-24573	18	13	(	(	PUNCT
brj-24573	18	14	kumar	kumar	PROPN
brj-24573	18	15	et	et	PROPN
brj-24573	18	16	al	al	PROPN
brj-24573	18	17	.	.	PROPN
brj-24573	18	18	2013	2013	NUM
brj-24573	18	19	;	;	PUNCT
brj-24573	18	20	van	van	PROPN
brj-24573	18	21	dijl	dijl	PROPN
brj-24573	18	22	and	and	CCONJ
brj-24573	18	23	hecker	hecker	NOUN
brj-24573	18	24	2013	2013	NUM
brj-24573	18	25	)	)	PUNCT
brj-24573	18	26	because	because	SCONJ
brj-24573	18	27	the	the	DET
brj-24573	18	28	extracellular	extracellular	ADJ
brj-24573	18	29	proteins	protein	NOUN
brj-24573	18	30	synthesized	synthesize	VERB
brj-24573	18	31	by	by	ADP
brj-24573	18	32	bacillus	bacillus	NOUN
brj-24573	18	33	are	be	AUX
brj-24573	18	34	directly	directly	ADV
brj-24573	18	35	secreted	secrete	VERB
brj-24573	18	36	into	into	ADP
brj-24573	18	37	the	the	DET
brj-24573	18	38	culture	culture	NOUN
brj-24573	18	39	https://orcid.org/0009-0007-0095-2270	https://orcid.org/0009-0007-0095-2270	PROPN
brj-24573	18	40	https://orcid.org/0000-0002-2430-8646	https://orcid.org/0000-0002-2430-8646	VERB
brj-24573	18	41	https://orcid.org/0009-0000-1597-8560?lang=en	https://orcid.org/0009-0000-1597-8560?lang=en	PROPN
brj-24573	18	42	https://orcid.org/0000-0002-2361-4959	https://orcid.org/0000-0002-2361-4959	PROPN
brj-24573	18	43	https://orcid.org/0000-0002-0675-9849	https://orcid.org/0000-0002-0675-9849	PROPN
brj-24573	18	44	peer	peer	NOUN
brj-24573	18	45	-	-	PUNCT
brj-24573	18	46	reviewed	review	VERB
brj-24573	18	47	article	article	NOUN
brj-24573	18	48	bioresources.cnr.ncsu.edu	bioresources.cnr.ncsu.edu	X
brj-24573	18	49	farouk	farouk	PROPN
brj-24573	18	50	et	et	PROPN
brj-24573	18	51	al	al	PROPN
brj-24573	18	52	.	.	PROPN
brj-24573	19	1	(	(	PUNCT
brj-24573	19	2	2025	2025	NUM
brj-24573	19	3	)	)	PUNCT
brj-24573	19	4	.	.	PUNCT
brj-24573	20	1	“	"	PUNCT
brj-24573	20	2	amylase	amylase	NOUN
brj-24573	20	3	via	via	ADP
brj-24573	20	4	xylose	xylose	PROPN
brj-24573	20	5	promoter	promoter	NOUN
brj-24573	20	6	,	,	PUNCT
brj-24573	20	7	”	"	PUNCT
brj-24573	20	8	bioresources	bioresource	NOUN
brj-24573	20	9	20(3	20(3	NOUN
brj-24573	20	10	)	)	PUNCT
brj-24573	20	11	,	,	PUNCT
brj-24573	20	12	7728	7728	NUM
brj-24573	20	13	-	-	SYM
brj-24573	20	14	7737	7737	NUM
brj-24573	20	15	.	.	PUNCT
brj-24573	21	1	7729	7729	NUM
brj-24573	21	2	media	medium	NOUN
brj-24573	21	3	(	(	PUNCT
brj-24573	21	4	liu	liu	PROPN
brj-24573	21	5	et	et	PROPN
brj-24573	21	6	al	al	PROPN
brj-24573	21	7	.	.	PROPN
brj-24573	21	8	2022	2022	NUM
brj-24573	21	9	)	)	PUNCT
brj-24573	21	10	.	.	PUNCT
brj-24573	22	1	thus	thus	ADV
brj-24573	22	2	,	,	PUNCT
brj-24573	22	3	inducible	inducible	ADJ
brj-24573	22	4	expression	expression	NOUN
brj-24573	22	5	systems	system	NOUN
brj-24573	22	6	in	in	ADP
brj-24573	22	7	b.	b.	PROPN
brj-24573	22	8	subtilis	subtilis	PROPN
brj-24573	22	9	provide	provide	VERB
brj-24573	22	10	advantages	advantage	NOUN
brj-24573	22	11	especially	especially	ADV
brj-24573	22	12	for	for	ADP
brj-24573	22	13	the	the	DET
brj-24573	22	14	expression	expression	NOUN
brj-24573	22	15	of	of	ADP
brj-24573	22	16	foreign	foreign	ADJ
brj-24573	22	17	genes	gene	NOUN
brj-24573	22	18	that	that	PRON
brj-24573	22	19	might	might	AUX
brj-24573	22	20	encode	encode	VERB
brj-24573	22	21	lethal	lethal	ADJ
brj-24573	22	22	or	or	CCONJ
brj-24573	22	23	detrimental	detrimental	ADJ
brj-24573	22	24	gene	gene	NOUN
brj-24573	22	25	products	product	NOUN
brj-24573	22	26	.	.	PUNCT
brj-24573	23	1	under	under	ADP
brj-24573	23	2	repression	repression	NOUN
brj-24573	23	3	conditions	condition	NOUN
brj-24573	23	4	,	,	PUNCT
brj-24573	23	5	the	the	DET
brj-24573	23	6	foreign	foreign	ADJ
brj-24573	23	7	gene	gene	NOUN
brj-24573	23	8	can	can	AUX
brj-24573	23	9	be	be	AUX
brj-24573	23	10	stably	stably	ADV
brj-24573	23	11	maintained	maintain	VERB
brj-24573	23	12	without	without	ADP
brj-24573	23	13	selection	selection	NOUN
brj-24573	23	14	pressure	pressure	NOUN
brj-24573	23	15	.	.	PUNCT
brj-24573	24	1	after	after	ADP
brj-24573	24	2	the	the	DET
brj-24573	24	3	induction	induction	NOUN
brj-24573	24	4	of	of	ADP
brj-24573	24	5	the	the	DET
brj-24573	24	6	gene	gene	NOUN
brj-24573	24	7	of	of	ADP
brj-24573	24	8	interest	interest	NOUN
brj-24573	24	9	,	,	PUNCT
brj-24573	24	10	regulated	regulated	ADJ
brj-24573	24	11	promoters	promoter	NOUN
brj-24573	24	12	allow	allow	VERB
brj-24573	24	13	the	the	DET
brj-24573	24	14	production	production	NOUN
brj-24573	24	15	of	of	ADP
brj-24573	24	16	even	even	ADV
brj-24573	24	17	toxic	toxic	ADJ
brj-24573	24	18	proteins	protein	NOUN
brj-24573	24	19	for	for	ADP
brj-24573	24	20	a	a	DET
brj-24573	24	21	limited	limited	ADJ
brj-24573	24	22	period	period	NOUN
brj-24573	24	23	of	of	ADP
brj-24573	24	24	growth	growth	NOUN
brj-24573	24	25	.	.	PUNCT
brj-24573	25	1	moreover	moreover	ADV
brj-24573	25	2	,	,	PUNCT
brj-24573	25	3	the	the	DET
brj-24573	25	4	regulated	regulated	ADJ
brj-24573	25	5	expression	expression	NOUN
brj-24573	25	6	also	also	ADV
brj-24573	25	7	minimizes	minimize	VERB
brj-24573	25	8	the	the	DET
brj-24573	25	9	exposure	exposure	NOUN
brj-24573	25	10	of	of	ADP
brj-24573	25	11	the	the	DET
brj-24573	25	12	foreign	foreign	ADJ
brj-24573	25	13	gene	gene	NOUN
brj-24573	25	14	products	product	NOUN
brj-24573	25	15	to	to	ADP
brj-24573	25	16	proteases	protease	NOUN
brj-24573	25	17	that	that	PRON
brj-24573	25	18	are	be	AUX
brj-24573	25	19	usually	usually	ADV
brj-24573	25	20	co	co	VERB
brj-24573	25	21	-	-	VERB
brj-24573	25	22	synthesized	synthesize	VERB
brj-24573	25	23	in	in	ADP
brj-24573	25	24	b.	b.	PROPN
brj-24573	25	25	subtilis	subtilis	PROPN
brj-24573	25	26	,	,	PUNCT
brj-24573	25	27	either	either	CCONJ
brj-24573	25	28	as	as	ADP
brj-24573	25	29	intracellular	intracellular	ADJ
brj-24573	25	30	or	or	CCONJ
brj-24573	25	31	secreted	secrete	VERB
brj-24573	25	32	enzymes	enzyme	NOUN
brj-24573	25	33	.	.	PUNCT
brj-24573	26	1	several	several	ADJ
brj-24573	26	2	inducible	inducible	ADJ
brj-24573	26	3	promoter	promoter	NOUN
brj-24573	26	4	systems	system	NOUN
brj-24573	26	5	have	have	AUX
brj-24573	26	6	been	be	AUX
brj-24573	26	7	developed	develop	VERB
brj-24573	26	8	for	for	ADP
brj-24573	26	9	use	use	NOUN
brj-24573	26	10	in	in	ADP
brj-24573	26	11	a	a	DET
brj-24573	26	12	bacillus	bacillus	NOUN
brj-24573	26	13	expression	expression	NOUN
brj-24573	26	14	system	system	NOUN
brj-24573	26	15	.	.	PUNCT
brj-24573	27	1	one	one	NUM
brj-24573	27	2	of	of	ADP
brj-24573	27	3	the	the	DET
brj-24573	27	4	first	first	ADJ
brj-24573	27	5	expression	expression	NOUN
brj-24573	27	6	systems	system	NOUN
brj-24573	27	7	used	use	VERB
brj-24573	27	8	the	the	DET
brj-24573	27	9	iptg	iptg	NOUN
brj-24573	27	10	inducible	inducible	ADJ
brj-24573	27	11	lac	lac	PROPN
brj-24573	27	12	repressor	repressor	NOUN
brj-24573	27	13	/	/	SYM
brj-24573	27	14	operator	operator	NOUN
brj-24573	27	15	elements	element	NOUN
brj-24573	27	16	of	of	ADP
brj-24573	27	17	the	the	DET
brj-24573	27	18	e.	e.	PROPN
brj-24573	27	19	coli	coli	PROPN
brj-24573	27	20	lac	lac	PROPN
brj-24573	27	21	operon	operon	PROPN
brj-24573	27	22	in	in	ADP
brj-24573	27	23	b.	b.	PROPN
brj-24573	27	24	subtilis	subtilis	PROPN
brj-24573	27	25	(	(	PUNCT
brj-24573	27	26	yansura	yansura	NOUN
brj-24573	27	27	and	and	CCONJ
brj-24573	27	28	henner	henner	PROPN
brj-24573	27	29	1984	1984	NUM
brj-24573	27	30	;	;	PUNCT
brj-24573	27	31	terpe	terpe	NOUN
brj-24573	27	32	2006	2006	NUM
brj-24573	27	33	)	)	PUNCT
brj-24573	27	34	.	.	PUNCT
brj-24573	28	1	in	in	ADP
brj-24573	28	2	addition	addition	NOUN
brj-24573	28	3	,	,	PUNCT
brj-24573	28	4	various	various	ADJ
brj-24573	28	5	other	other	ADJ
brj-24573	28	6	sugar	sugar	NOUN
brj-24573	28	7	inducible	inducible	ADJ
brj-24573	28	8	systems	system	NOUN
brj-24573	28	9	were	be	AUX
brj-24573	28	10	established	establish	VERB
brj-24573	28	11	in	in	ADP
brj-24573	28	12	b.	b.	PROPN
brj-24573	28	13	subtilis	subtilis	PROPN
brj-24573	28	14	.	.	PUNCT
brj-24573	29	1	they	they	PRON
brj-24573	29	2	are	be	AUX
brj-24573	29	3	derived	derive	VERB
brj-24573	29	4	from	from	ADP
brj-24573	29	5	the	the	DET
brj-24573	29	6	glucanate	glucanate	NOUN
brj-24573	29	7	(	(	PUNCT
brj-24573	29	8	glt	glt	PROPN
brj-24573	29	9	)	)	PUNCT
brj-24573	29	10	operon	operon	NOUN
brj-24573	29	11	(	(	PUNCT
brj-24573	29	12	reizer	reizer	NOUN
brj-24573	29	13	et	et	PROPN
brj-24573	29	14	al	al	PROPN
brj-24573	29	15	.	.	PROPN
brj-24573	29	16	1991	1991	NUM
brj-24573	29	17	)	)	PUNCT
brj-24573	29	18	,	,	PUNCT
brj-24573	29	19	the	the	DET
brj-24573	29	20	sucrose	sucrose	NOUN
brj-24573	29	21	inducible	inducible	ADJ
brj-24573	29	22	sacb	sacb	PROPN
brj-24573	29	23	operon	operon	PROPN
brj-24573	29	24	(	(	PUNCT
brj-24573	29	25	pereira	pereira	PROPN
brj-24573	29	26	et	et	PROPN
brj-24573	29	27	al	al	PROPN
brj-24573	29	28	.	.	PROPN
brj-24573	29	29	2001	2001	NUM
brj-24573	29	30	)	)	PUNCT
brj-24573	29	31	,	,	PUNCT
brj-24573	29	32	or	or	CCONJ
brj-24573	29	33	the	the	DET
brj-24573	29	34	inducible	inducible	ADJ
brj-24573	29	35	xylose	xylose	PROPN
brj-24573	29	36	isomerase	isomerase	ADJ
brj-24573	29	37	gene	gene	NOUN
brj-24573	29	38	of	of	ADP
brj-24573	29	39	different	different	ADJ
brj-24573	29	40	bacillus	bacillus	NOUN
brj-24573	29	41	species	specie	NOUN
brj-24573	29	42	as	as	ADP
brj-24573	29	43	those	those	PRON
brj-24573	29	44	from	from	ADP
brj-24573	29	45	b.	b.	PROPN
brj-24573	29	46	megaterium	megaterium	PROPN
brj-24573	29	47	(	(	PUNCT
brj-24573	29	48	rygus	rygus	ADJ
brj-24573	29	49	and	and	CCONJ
brj-24573	29	50	hillen	hillen	ADJ
brj-24573	29	51	1991a	1991a	NUM
brj-24573	29	52	)	)	PUNCT
brj-24573	29	53	or	or	CCONJ
brj-24573	29	54	b.	b.	PROPN
brj-24573	29	55	subtilis	subtilis	PROPN
brj-24573	29	56	(	(	PUNCT
brj-24573	29	57	kim	kim	PROPN
brj-24573	29	58	et	et	PROPN
brj-24573	29	59	al	al	PROPN
brj-24573	29	60	.	.	PROPN
brj-24573	29	61	1996	1996	NUM
brj-24573	29	62	)	)	PUNCT
brj-24573	29	63	.	.	PUNCT
brj-24573	30	1	for	for	ADP
brj-24573	30	2	quite	quite	ADV
brj-24573	30	3	distinct	distinct	ADJ
brj-24573	30	4	reasons	reason	NOUN
brj-24573	30	5	,	,	PUNCT
brj-24573	30	6	secretion	secretion	NOUN
brj-24573	30	7	of	of	ADP
brj-24573	30	8	heterologous	heterologous	ADJ
brj-24573	30	9	proteins	protein	NOUN
brj-24573	30	10	into	into	ADP
brj-24573	30	11	the	the	DET
brj-24573	30	12	culture	culture	NOUN
brj-24573	30	13	media	medium	NOUN
brj-24573	30	14	by	by	ADP
brj-24573	30	15	b.	b.	PROPN
brj-24573	30	16	subtilis	subtilis	PROPN
brj-24573	30	17	is	be	AUX
brj-24573	30	18	also	also	ADV
brj-24573	30	19	highly	highly	ADV
brj-24573	30	20	recommended	recommend	VERB
brj-24573	30	21	(	(	PUNCT
brj-24573	30	22	wong	wong	PROPN
brj-24573	30	23	1995	1995	NUM
brj-24573	30	24	)	)	PUNCT
brj-24573	30	25	.	.	PUNCT
brj-24573	31	1	keeping	keep	VERB
brj-24573	31	2	into	into	ADP
brj-24573	31	3	consideration	consideration	NOUN
brj-24573	31	4	the	the	DET
brj-24573	31	5	advantages	advantage	NOUN
brj-24573	31	6	of	of	ADP
brj-24573	31	7	bacillus	bacillus	NOUN
brj-24573	31	8	expression	expression	NOUN
brj-24573	31	9	system	system	NOUN
brj-24573	31	10	and	and	CCONJ
brj-24573	31	11	importance	importance	NOUN
brj-24573	31	12	of	of	ADP
brj-24573	31	13	amylases	amylase	NOUN
brj-24573	31	14	,	,	PUNCT
brj-24573	31	15	the	the	DET
brj-24573	31	16	present	present	ADJ
brj-24573	31	17	study	study	NOUN
brj-24573	31	18	aims	aim	VERB
brj-24573	31	19	at	at	ADP
brj-24573	31	20	developing	develop	VERB
brj-24573	31	21	a	a	DET
brj-24573	31	22	xylose	xylose	PROPN
brj-24573	31	23	-	-	PUNCT
brj-24573	31	24	inducible	inducible	ADJ
brj-24573	31	25	system	system	NOUN
brj-24573	31	26	to	to	PART
brj-24573	31	27	produce	produce	VERB
brj-24573	31	28	recombinant	recombinant	ADJ
brj-24573	31	29	α	α	NOUN
brj-24573	31	30	-	-	NOUN
brj-24573	31	31	amylase	amylase	NOUN
brj-24573	31	32	derived	derive	VERB
brj-24573	31	33	from	from	ADP
brj-24573	31	34	bacterium	bacterium	NOUN
brj-24573	31	35	thermoactinomyces	thermoactinomyce	NOUN
brj-24573	31	36	vulgaris	vulgaris	NOUN
brj-24573	31	37	94	94	NUM
brj-24573	31	38	-	-	SYM
brj-24573	31	39	2a	2a	NUM
brj-24573	31	40	(	(	PUNCT
brj-24573	31	41	amytv	amytv	NOUN
brj-24573	31	42	)	)	PUNCT
brj-24573	31	43	within	within	ADP
brj-24573	31	44	a	a	DET
brj-24573	31	45	bacillus	bacillus	NOUN
brj-24573	31	46	expression	expression	NOUN
brj-24573	31	47	system	system	NOUN
brj-24573	31	48	.	.	PUNCT
brj-24573	32	1	experimental	experimental	ADJ
brj-24573	32	2	bacterial	bacterial	ADJ
brj-24573	32	3	strains	strain	NOUN
brj-24573	32	4	,	,	PUNCT
brj-24573	32	5	plasmids	plasmid	NOUN
brj-24573	32	6	,	,	PUNCT
brj-24573	32	7	media	medium	NOUN
brj-24573	32	8	,	,	PUNCT
brj-24573	32	9	and	and	CCONJ
brj-24573	32	10	chemicals	chemical	NOUN
brj-24573	32	11	e.	e.	PROPN
brj-24573	32	12	coli	coli	PROPN
brj-24573	32	13	dh5α	dh5α	PROPN
brj-24573	32	14	(	(	PUNCT
brj-24573	32	15	stratagene	stratagene	NOUN
brj-24573	32	16	,	,	PUNCT
brj-24573	32	17	lajolla	lajolla	PROPN
brj-24573	32	18	,	,	PUNCT
brj-24573	32	19	ca	ca	NOUN
brj-24573	32	20	)	)	PUNCT
brj-24573	32	21	was	be	AUX
brj-24573	32	22	utilized	utilize	VERB
brj-24573	32	23	as	as	ADP
brj-24573	32	24	host	host	NOUN
brj-24573	32	25	for	for	ADP
brj-24573	32	26	transformation	transformation	NOUN
brj-24573	32	27	.	.	PUNCT
brj-24573	33	1	the	the	DET
brj-24573	33	2	target	target	NOUN
brj-24573	33	3	protein	protein	NOUN
brj-24573	33	4	was	be	AUX
brj-24573	33	5	expressed	express	VERB
brj-24573	33	6	and	and	CCONJ
brj-24573	33	7	secreted	secrete	VERB
brj-24573	33	8	using	use	VERB
brj-24573	33	9	the	the	DET
brj-24573	33	10	bacillus	bacillus	NOUN
brj-24573	33	11	subtilis	subtilis	NOUN
brj-24573	33	12	(	(	PUNCT
brj-24573	33	13	gsb26	gsb26	NOUN
brj-24573	33	14	)	)	PUNCT
brj-24573	33	15	strain	strain	NOUN
brj-24573	33	16	.	.	PUNCT
brj-24573	34	1	for	for	ADP
brj-24573	34	2	direct	direct	ADJ
brj-24573	34	3	pcr	pcr	NOUN
brj-24573	34	4	cloning	cloning	NOUN
brj-24573	34	5	and	and	CCONJ
brj-24573	34	6	nucleotide	nucleotide	ADJ
brj-24573	34	7	sequencing	sequencing	NOUN
brj-24573	34	8	,	,	PUNCT
brj-24573	34	9	pgem	pgem	PROPN
brj-24573	34	10	™	™	X
brj-24573	34	11	-t	-t	X
brj-24573	34	12	vector	vector	NOUN
brj-24573	34	13	(	(	PUNCT
brj-24573	34	14	thermo	thermo	NOUN
brj-24573	34	15	fisher	fisher	PROPN
brj-24573	34	16	scientific	scientific	PROPN
brj-24573	34	17	)	)	PUNCT
brj-24573	34	18	was	be	AUX
brj-24573	34	19	employed	employ	VERB
brj-24573	34	20	.	.	PUNCT
brj-24573	35	1	the	the	DET
brj-24573	35	2	growth	growth	NOUN
brj-24573	35	3	of	of	ADP
brj-24573	35	4	b.	b.	PROPN
brj-24573	35	5	subtilis	subtilis	PROPN
brj-24573	35	6	was	be	AUX
brj-24573	35	7	achieved	achieve	VERB
brj-24573	35	8	in	in	ADP
brj-24573	35	9	low	low	ADJ
brj-24573	35	10	phosphate	phosphate	NOUN
brj-24573	35	11	culture	culture	NOUN
brj-24573	35	12	media	medium	NOUN
brj-24573	35	13	(	(	PUNCT
brj-24573	35	14	2x	2x	NUM
brj-24573	35	15	tby	tby	NOUN
brj-24573	35	16	having	have	VERB
brj-24573	35	17	composition	composition	NOUN
brj-24573	35	18	as	as	ADP
brj-24573	35	19	:	:	PUNCT
brj-24573	35	20	tryptone	tryptone	NOUN
brj-24573	35	21	1.6	1.6	NUM
brj-24573	35	22	%	%	NOUN
brj-24573	35	23	,	,	PUNCT
brj-24573	35	24	yeast	yeast	NOUN
brj-24573	35	25	extract	extract	VERB
brj-24573	35	26	1	1	NUM
brj-24573	35	27	%	%	NOUN
brj-24573	35	28	,	,	PUNCT
brj-24573	35	29	and	and	CCONJ
brj-24573	35	30	nacl	nacl	NOUN
brj-24573	35	31	0.5	0.5	NUM
brj-24573	35	32	%	%	NOUN
brj-24573	35	33	)	)	PUNCT
brj-24573	35	34	.	.	PUNCT
brj-24573	36	1	to	to	PART
brj-24573	36	2	ensure	ensure	VERB
brj-24573	36	3	proper	proper	ADJ
brj-24573	36	4	selection	selection	NOUN
brj-24573	36	5	and	and	CCONJ
brj-24573	36	6	maintenance	maintenance	NOUN
brj-24573	36	7	of	of	ADP
brj-24573	36	8	transformed	transform	VERB
brj-24573	36	9	cells	cell	NOUN
brj-24573	36	10	,	,	PUNCT
brj-24573	36	11	antibiotics	antibiotic	NOUN
brj-24573	36	12	(	(	PUNCT
brj-24573	36	13	obtained	obtain	VERB
brj-24573	36	14	from	from	ADP
brj-24573	36	15	sigma	sigma	PROPN
brj-24573	36	16	-	-	PUNCT
brj-24573	36	17	aldrich	aldrich	NOUN
brj-24573	36	18	)	)	PUNCT
brj-24573	36	19	were	be	AUX
brj-24573	36	20	added	add	VERB
brj-24573	36	21	to	to	ADP
brj-24573	36	22	the	the	DET
brj-24573	36	23	growth	growth	NOUN
brj-24573	36	24	medium	medium	NOUN
brj-24573	36	25	at	at	ADP
brj-24573	36	26	specific	specific	ADJ
brj-24573	36	27	concentrations	concentration	NOUN
brj-24573	36	28	:	:	PUNCT
brj-24573	36	29	ampicillin	ampicillin	NOUN
brj-24573	36	30	(	(	PUNCT
brj-24573	36	31	100	100	NUM
brj-24573	36	32	µg	µg	ADJ
brj-24573	36	33	/	/	SYM
brj-24573	36	34	ml	ml	NOUN
brj-24573	36	35	)	)	PUNCT
brj-24573	36	36	and	and	CCONJ
brj-24573	36	37	tetracycline	tetracycline	PROPN
brj-24573	36	38	(	(	PUNCT
brj-24573	36	39	10	10	NUM
brj-24573	36	40	mg	mg	NUM
brj-24573	36	41	/	/	SYM
brj-24573	36	42	ml	ml	NOUN
brj-24573	36	43	)	)	PUNCT
brj-24573	36	44	.	.	PUNCT
brj-24573	37	1	starch	starch	NOUN
brj-24573	37	2	used	use	VERB
brj-24573	37	3	in	in	ADP
brj-24573	37	4	the	the	DET
brj-24573	37	5	study	study	NOUN
brj-24573	37	6	was	be	AUX
brj-24573	37	7	purchased	purchase	VERB
brj-24573	37	8	from	from	ADP
brj-24573	37	9	sigma	sigma	PROPN
brj-24573	37	10	-	-	PUNCT
brj-24573	37	11	aldrich	aldrich	PROPN
brj-24573	37	12	(	(	PUNCT
brj-24573	37	13	steinheim	steinheim	PROPN
brj-24573	37	14	,	,	PUNCT
brj-24573	37	15	germany	germany	PROPN
brj-24573	37	16	)	)	PUNCT
brj-24573	37	17	while	while	SCONJ
brj-24573	37	18	the	the	DET
brj-24573	37	19	amylase	amylase	NOUN
brj-24573	37	20	assay	assay	NOUN
brj-24573	37	21	kit	kit	NOUN
brj-24573	37	22	was	be	AUX
brj-24573	37	23	obtained	obtain	VERB
brj-24573	37	24	from	from	ADP
brj-24573	37	25	sigma	sigma	PROPN
brj-24573	37	26	,	,	PUNCT
brj-24573	37	27	usa	usa	PROPN
brj-24573	37	28	.	.	PROPN
brj-24573	37	29	construction	construction	NOUN
brj-24573	37	30	of	of	ADP
brj-24573	37	31	gene	gene	NOUN
brj-24573	37	32	cassette	cassette	NOUN
brj-24573	37	33	encoding	encode	VERB
brj-24573	37	34	α	α	NOUN
brj-24573	37	35	-	-	NOUN
brj-24573	37	36	amylase	amylase	NOUN
brj-24573	37	37	from	from	ADP
brj-24573	37	38	t.	t.	NOUN
brj-24573	37	39	vulgaris	vulgaris	NOUN
brj-24573	37	40	the	the	DET
brj-24573	37	41	gene	gene	NOUN
brj-24573	37	42	encoding	encode	VERB
brj-24573	37	43	α	α	NOUN
brj-24573	37	44	-	-	NOUN
brj-24573	37	45	amylase	amylase	NOUN
brj-24573	37	46	from	from	ADP
brj-24573	37	47	t.	t.	NOUN
brj-24573	37	48	vulgaris	vulgaris	NOUN
brj-24573	37	49	was	be	AUX
brj-24573	37	50	cloned	clone	VERB
brj-24573	37	51	following	follow	VERB
brj-24573	37	52	protocol	protocol	NOUN
brj-24573	37	53	by	by	ADP
brj-24573	37	54	hofemeister	hofemeister	PROPN
brj-24573	37	55	et	et	PROPN
brj-24573	37	56	al	al	PROPN
brj-24573	37	57	.	.	PROPN
brj-24573	38	1	(	(	PUNCT
brj-24573	38	2	1994	1994	NUM
brj-24573	38	3	)	)	PUNCT
brj-24573	38	4	,	,	PUNCT
brj-24573	38	5	followed	follow	VERB
brj-24573	38	6	by	by	ADP
brj-24573	38	7	subcloning	subclone	VERB
brj-24573	38	8	in	in	ADP
brj-24573	38	9	pgem	pgem	PROPN
brj-24573	38	10	™	™	PART
brj-24573	38	11	-t	-t	X
brj-24573	38	12	vector	vector	NOUN
brj-24573	38	13	.	.	PUNCT
brj-24573	39	1	the	the	DET
brj-24573	39	2	sequence	sequence	NOUN
brj-24573	39	3	of	of	ADP
brj-24573	39	4	the	the	DET
brj-24573	39	5	genes	gene	NOUN
brj-24573	39	6	was	be	AUX
brj-24573	39	7	determined	determine	VERB
brj-24573	39	8	using	use	VERB
brj-24573	39	9	standard	standard	ADJ
brj-24573	39	10	dideoxy	dideoxy	ADJ
brj-24573	39	11	chain	chain	NOUN
brj-24573	39	12	termination	termination	NOUN
brj-24573	39	13	method	method	NOUN
brj-24573	39	14	using	use	VERB
brj-24573	39	15	the	the	DET
brj-24573	39	16	t7	t7	PROPN
brj-24573	39	17	promoter	promoter	NOUN
brj-24573	39	18	and	and	CCONJ
brj-24573	39	19	t7	t7	PROPN
brj-24573	39	20	terminator	terminator	NOUN
brj-24573	39	21	primers	primer	NOUN
brj-24573	39	22	in	in	ADP
brj-24573	39	23	t7	t7	PROPN
brj-24573	39	24	dna	dna	PROPN
brj-24573	39	25	sequenase	sequenase	PROPN
brj-24573	39	26	kit	kit	PROPN
brj-24573	39	27	.	.	PUNCT
brj-24573	40	1	the	the	DET
brj-24573	40	2	gene	gene	NOUN
brj-24573	40	3	was	be	AUX
brj-24573	40	4	identified	identify	VERB
brj-24573	40	5	through	through	ADP
brj-24573	40	6	sequencing	sequence	VERB
brj-24573	40	7	and	and	CCONJ
brj-24573	40	8	subsequently	subsequently	ADV
brj-24573	40	9	cloned	clone	VERB
brj-24573	40	10	into	into	ADP
brj-24573	40	11	smai	smai	NOUN
brj-24573	40	12	linearized	linearize	VERB
brj-24573	40	13	b.	b.	PROPN
brj-24573	40	14	megaterium	megaterium	PROPN
brj-24573	40	15	pwh1520	pwh1520	PROPN
brj-24573	40	16	vector	vector	NOUN
brj-24573	40	17	having	have	VERB
brj-24573	40	18	xylose	xylose	PROPN
brj-24573	40	19	inducible	inducible	ADJ
brj-24573	40	20	promoter	promoter	NOUN
brj-24573	40	21	(	(	PUNCT
brj-24573	40	22	pxyla	pxyla	NOUN
brj-24573	40	23	)	)	PUNCT
brj-24573	40	24	and	and	CCONJ
brj-24573	40	25	xylose	xylose	PROPN
brj-24573	40	26	repressor	repressor	NOUN
brj-24573	40	27	gene	gene	NOUN
brj-24573	40	28	(	(	PUNCT
brj-24573	40	29	xylr	xylr	PROPN
brj-24573	40	30	)	)	PUNCT
brj-24573	40	31	.	.	PUNCT
brj-24573	41	1	the	the	DET
brj-24573	41	2	resultant	resultant	NOUN
brj-24573	41	3	plasmid	plasmid	NOUN
brj-24573	41	4	was	be	AUX
brj-24573	41	5	named	name	VERB
brj-24573	41	6	xyla	xyla	ADV
brj-24573	41	7	-	-	PUNCT
brj-24573	41	8	amy(tv	amy(tv	VERB
brj-24573	41	9	)	)	PUNCT
brj-24573	41	10	.	.	PUNCT
brj-24573	42	1	successful	successful	ADJ
brj-24573	42	2	cloning	cloning	NOUN
brj-24573	42	3	was	be	AUX
brj-24573	42	4	verified	verify	VERB
brj-24573	42	5	by	by	ADP
brj-24573	42	6	restriction	restriction	NOUN
brj-24573	42	7	digestion	digestion	NOUN
brj-24573	42	8	analysis	analysis	NOUN
brj-24573	42	9	using	use	VERB
brj-24573	42	10	msti	msti	NOUN
brj-24573	42	11	and	and	CCONJ
brj-24573	42	12	hindiii	hindiii	NOUN
brj-24573	42	13	.	.	PUNCT
brj-24573	43	1	peer	peer	NOUN
brj-24573	43	2	-	-	PUNCT
brj-24573	43	3	reviewed	review	VERB
brj-24573	43	4	article	article	NOUN
brj-24573	43	5	bioresources.cnr.ncsu.edu	bioresources.cnr.ncsu.edu	X
brj-24573	43	6	farouk	farouk	PROPN
brj-24573	43	7	et	et	PROPN
brj-24573	43	8	al	al	PROPN
brj-24573	43	9	.	.	PROPN
brj-24573	44	1	(	(	PUNCT
brj-24573	44	2	2025	2025	NUM
brj-24573	44	3	)	)	PUNCT
brj-24573	44	4	.	.	PUNCT
brj-24573	45	1	“	"	PUNCT
brj-24573	45	2	amylase	amylase	NOUN
brj-24573	45	3	via	via	ADP
brj-24573	45	4	xylose	xylose	PROPN
brj-24573	45	5	promoter	promoter	NOUN
brj-24573	45	6	,	,	PUNCT
brj-24573	45	7	”	"	PUNCT
brj-24573	45	8	bioresources	bioresource	NOUN
brj-24573	45	9	20(3	20(3	NOUN
brj-24573	45	10	)	)	PUNCT
brj-24573	45	11	,	,	PUNCT
brj-24573	45	12	7728	7728	NUM
brj-24573	45	13	-	-	SYM
brj-24573	45	14	7737	7737	NUM
brj-24573	45	15	.	.	PUNCT
brj-24573	46	1	7730	7730	NUM
brj-24573	46	2	expression	expression	NOUN
brj-24573	46	3	analysis	analysis	NOUN
brj-24573	46	4	and	and	CCONJ
brj-24573	46	5	protein	protein	NOUN
brj-24573	46	6	quantification	quantification	NOUN
brj-24573	46	7	to	to	PART
brj-24573	46	8	obtain	obtain	VERB
brj-24573	46	9	a	a	DET
brj-24573	46	10	heterologous	heterologous	ADJ
brj-24573	46	11	expression	expression	NOUN
brj-24573	46	12	of	of	ADP
brj-24573	46	13	amytv	amytv	NOUN
brj-24573	46	14	gene	gene	NOUN
brj-24573	46	15	,	,	PUNCT
brj-24573	46	16	bacillus	bacillus	NOUN
brj-24573	46	17	subtilis	subtili	NOUN
brj-24573	46	18	(	(	PUNCT
brj-24573	46	19	gsb26	gsb26	NOUN
brj-24573	46	20	)	)	PUNCT
brj-24573	46	21	cells	cell	NOUN
brj-24573	46	22	were	be	AUX
brj-24573	46	23	transformed	transform	VERB
brj-24573	46	24	using	use	VERB
brj-24573	46	25	xyla	xyla	NOUN
brj-24573	46	26	-	-	PUNCT
brj-24573	46	27	amy(tv	amy(tv	VERB
brj-24573	46	28	)	)	PUNCT
brj-24573	46	29	recombinant	recombinant	ADJ
brj-24573	46	30	plasmid	plasmid	NOUN
brj-24573	46	31	.	.	PUNCT
brj-24573	47	1	after	after	ADP
brj-24573	47	2	induction	induction	NOUN
brj-24573	47	3	with	with	ADP
brj-24573	47	4	xylose	xylose	PROPN
brj-24573	47	5	as	as	ADP
brj-24573	47	6	inducer	inducer	NOUN
brj-24573	47	7	,	,	PUNCT
brj-24573	47	8	the	the	DET
brj-24573	47	9	bacterial	bacterial	ADJ
brj-24573	47	10	cultures	culture	NOUN
brj-24573	47	11	were	be	AUX
brj-24573	47	12	cultivated	cultivate	VERB
brj-24573	47	13	in	in	ADP
brj-24573	47	14	2x	2x	NUM
brj-24573	47	15	tby	tby	NOUN
brj-24573	47	16	medium	medium	NOUN
brj-24573	47	17	for	for	ADP
brj-24573	47	18	different	different	ADJ
brj-24573	47	19	durations	duration	NOUN
brj-24573	47	20	to	to	PART
brj-24573	47	21	optimize	optimize	VERB
brj-24573	47	22	the	the	DET
brj-24573	47	23	suitable	suitable	ADJ
brj-24573	47	24	time	time	NOUN
brj-24573	47	25	for	for	ADP
brj-24573	47	26	protein	protein	NOUN
brj-24573	47	27	secretion	secretion	NOUN
brj-24573	47	28	.	.	PUNCT
brj-24573	48	1	the	the	DET
brj-24573	48	2	cells	cell	NOUN
brj-24573	48	3	and	and	CCONJ
brj-24573	48	4	extracellular	extracellular	ADJ
brj-24573	48	5	fractions	fraction	NOUN
brj-24573	48	6	were	be	AUX
brj-24573	48	7	separated	separate	VERB
brj-24573	48	8	by	by	ADP
brj-24573	48	9	centrifugation	centrifugation	NOUN
brj-24573	48	10	at	at	ADP
brj-24573	48	11	8000×g	8000×g	NUM
brj-24573	48	12	.	.	PUNCT
brj-24573	49	1	the	the	DET
brj-24573	49	2	obtained	obtain	VERB
brj-24573	49	3	cell	cell	NOUN
brj-24573	49	4	pellets	pellet	NOUN
brj-24573	49	5	were	be	AUX
brj-24573	49	6	washed	wash	VERB
brj-24573	49	7	with	with	ADP
brj-24573	49	8	sterile	sterile	ADJ
brj-24573	49	9	water	water	NOUN
brj-24573	49	10	and	and	CCONJ
brj-24573	49	11	subjected	subject	VERB
brj-24573	49	12	to	to	ADP
brj-24573	49	13	sonication	sonication	NOUN
brj-24573	49	14	for	for	ADP
brj-24573	49	15	cell	cell	NOUN
brj-24573	49	16	lysis	lysis	NOUN
brj-24573	49	17	.	.	PUNCT
brj-24573	50	1	proteins	protein	NOUN
brj-24573	50	2	obtained	obtain	VERB
brj-24573	50	3	through	through	ADP
brj-24573	50	4	cell	cell	NOUN
brj-24573	50	5	lysis	lysis	NOUN
brj-24573	50	6	(	(	PUNCT
brj-24573	50	7	comprising	comprise	VERB
brj-24573	50	8	both	both	CCONJ
brj-24573	50	9	soluble	soluble	ADJ
brj-24573	50	10	and	and	CCONJ
brj-24573	50	11	insoluble	insoluble	ADJ
brj-24573	50	12	fractions	fraction	NOUN
brj-24573	50	13	)	)	PUNCT
brj-24573	50	14	were	be	AUX
brj-24573	50	15	separated	separate	VERB
brj-24573	50	16	using	use	VERB
brj-24573	50	17	centrifugation	centrifugation	NOUN
brj-24573	50	18	at	at	ADP
brj-24573	50	19	16000×g	16000×g	NUM
brj-24573	50	20	.	.	PUNCT
brj-24573	51	1	all	all	DET
brj-24573	51	2	the	the	DET
brj-24573	51	3	fractions	fraction	NOUN
brj-24573	51	4	obtained	obtain	VERB
brj-24573	51	5	(	(	PUNCT
brj-24573	51	6	including	include	VERB
brj-24573	51	7	extracellular	extracellular	ADJ
brj-24573	51	8	,	,	PUNCT
brj-24573	51	9	soluble	soluble	ADJ
brj-24573	51	10	and	and	CCONJ
brj-24573	51	11	insoluble	insoluble	ADJ
brj-24573	51	12	fractions	fraction	NOUN
brj-24573	51	13	)	)	PUNCT
brj-24573	51	14	were	be	AUX
brj-24573	51	15	subjected	subject	VERB
brj-24573	51	16	to	to	ADP
brj-24573	51	17	protein	protein	NOUN
brj-24573	51	18	analysis	analysis	NOUN
brj-24573	51	19	using	use	VERB
brj-24573	51	20	denaturing	denaturing	NOUN
brj-24573	51	21	polyacrylamide	polyacrylamide	NOUN
brj-24573	51	22	gel	gel	NOUN
brj-24573	51	23	electrophoresis	electrophoresis	NOUN
brj-24573	51	24	(	(	PUNCT
brj-24573	51	25	sds	sds	NOUN
brj-24573	51	26	-	-	PUNCT
brj-24573	51	27	page	page	NOUN
brj-24573	51	28	)	)	PUNCT
brj-24573	51	29	and	and	CCONJ
brj-24573	51	30	visualized	visualize	VERB
brj-24573	51	31	by	by	ADP
brj-24573	51	32	staining	stain	VERB
brj-24573	51	33	the	the	DET
brj-24573	51	34	gel	gel	NOUN
brj-24573	51	35	with	with	ADP
brj-24573	51	36	g-250	g-250	PROPN
brj-24573	51	37	dye	dye	PROPN
brj-24573	51	38	.	.	PUNCT
brj-24573	52	1	protein	protein	NOUN
brj-24573	52	2	quantification	quantification	NOUN
brj-24573	52	3	was	be	AUX
brj-24573	52	4	done	do	VERB
brj-24573	52	5	using	use	VERB
brj-24573	52	6	the	the	DET
brj-24573	52	7	bradford	bradford	PROPN
brj-24573	52	8	assay	assay	PROPN
brj-24573	52	9	(	(	PUNCT
brj-24573	52	10	bradford	bradford	NOUN
brj-24573	52	11	1976	1976	NUM
brj-24573	52	12	)	)	PUNCT
brj-24573	52	13	using	use	VERB
brj-24573	52	14	bsa	bsa	PROPN
brj-24573	52	15	as	as	ADP
brj-24573	52	16	a	a	DET
brj-24573	52	17	standard	standard	NOUN
brj-24573	52	18	.	.	PUNCT
brj-24573	53	1	optimization	optimization	NOUN
brj-24573	53	2	of	of	ADP
brj-24573	53	3	induction	induction	NOUN
brj-24573	53	4	conditions	condition	NOUN
brj-24573	53	5	and	and	CCONJ
brj-24573	53	6	inducer	inducer	NOUN
brj-24573	53	7	concentration	concentration	NOUN
brj-24573	53	8	the	the	DET
brj-24573	53	9	xyla	xyla	ADV
brj-24573	53	10	-	-	PUNCT
brj-24573	53	11	amy(tv	amy(tv	VERB
brj-24573	53	12	)	)	PUNCT
brj-24573	53	13	cassette	cassette	NOUN
brj-24573	53	14	was	be	AUX
brj-24573	53	15	used	use	VERB
brj-24573	53	16	to	to	PART
brj-24573	53	17	optimize	optimize	VERB
brj-24573	53	18	the	the	DET
brj-24573	53	19	conditions	condition	NOUN
brj-24573	53	20	for	for	ADP
brj-24573	53	21	inducing	induce	VERB
brj-24573	53	22	amytv	amytv	NOUN
brj-24573	53	23	secretion	secretion	NOUN
brj-24573	53	24	in	in	ADP
brj-24573	53	25	b.	b.	PROPN
brj-24573	53	26	subtilis	subtilis	PROPN
brj-24573	53	27	.	.	PUNCT
brj-24573	54	1	the	the	DET
brj-24573	54	2	experiments	experiment	NOUN
brj-24573	54	3	aimed	aim	VERB
brj-24573	54	4	to	to	PART
brj-24573	54	5	determine	determine	VERB
brj-24573	54	6	the	the	DET
brj-24573	54	7	induction	induction	NOUN
brj-24573	54	8	conditions	condition	NOUN
brj-24573	54	9	,	,	PUNCT
brj-24573	54	10	ideal	ideal	ADJ
brj-24573	54	11	amount	amount	NOUN
brj-24573	54	12	of	of	ADP
brj-24573	54	13	inducer	inducer	NOUN
brj-24573	54	14	and	and	CCONJ
brj-24573	54	15	the	the	DET
brj-24573	54	16	optimal	optimal	ADJ
brj-24573	54	17	induction	induction	NOUN
brj-24573	54	18	time	time	NOUN
brj-24573	54	19	.	.	PUNCT
brj-24573	55	1	to	to	PART
brj-24573	55	2	conduct	conduct	VERB
brj-24573	55	3	the	the	DET
brj-24573	55	4	experiments	experiment	NOUN
brj-24573	55	5	,	,	PUNCT
brj-24573	55	6	overnight	overnight	ADJ
brj-24573	55	7	cultures	culture	NOUN
brj-24573	55	8	of	of	ADP
brj-24573	55	9	b.	b.	PROPN
brj-24573	55	10	subtilis	subtilis	PROPN
brj-24573	55	11	having	have	VERB
brj-24573	55	12	xyla	xyla	NOUN
brj-24573	55	13	-	-	PUNCT
brj-24573	55	14	amy(tv	amy(tv	VERB
brj-24573	55	15	)	)	PUNCT
brj-24573	55	16	cassette	cassette	NOUN
brj-24573	55	17	present	present	ADJ
brj-24573	55	18	in	in	ADP
brj-24573	55	19	them	they	PRON
brj-24573	55	20	were	be	AUX
brj-24573	55	21	diluted	dilute	VERB
brj-24573	55	22	100	100	NUM
brj-24573	55	23	-	-	NOUN
brj-24573	55	24	fold	fold	VERB
brj-24573	55	25	in	in	ADP
brj-24573	55	26	fresh	fresh	ADJ
brj-24573	55	27	growth	growth	NOUN
brj-24573	55	28	medium	medium	NOUN
brj-24573	55	29	,	,	PUNCT
brj-24573	55	30	and	and	CCONJ
brj-24573	55	31	10	10	NUM
brj-24573	55	32	mg	mg	NUM
brj-24573	55	33	/	/	SYM
brj-24573	55	34	ml	ml	PROPN
brj-24573	55	35	tetracycline	tetracycline	NOUN
brj-24573	55	36	was	be	AUX
brj-24573	55	37	added	add	VERB
brj-24573	55	38	.	.	PUNCT
brj-24573	56	1	the	the	DET
brj-24573	56	2	cultures	culture	NOUN
brj-24573	56	3	were	be	AUX
brj-24573	56	4	then	then	ADV
brj-24573	56	5	grown	grow	VERB
brj-24573	56	6	at	at	ADP
brj-24573	56	7	37	37	NUM
brj-24573	56	8	°	°	NOUN
brj-24573	56	9	c	c	NOUN
brj-24573	56	10	.	.	PUNCT
brj-24573	57	1	the	the	DET
brj-24573	57	2	inducer	inducer	NOUN
brj-24573	57	3	,	,	PUNCT
brj-24573	57	4	1	1	NUM
brj-24573	57	5	%	%	NOUN
brj-24573	57	6	xylose	xylose	PROPN
brj-24573	57	7	,	,	PUNCT
brj-24573	57	8	was	be	AUX
brj-24573	57	9	added	add	VERB
brj-24573	57	10	at	at	ADP
brj-24573	57	11	various	various	ADJ
brj-24573	57	12	optical	optical	ADJ
brj-24573	57	13	density	density	NOUN
brj-24573	57	14	(	(	PUNCT
brj-24573	57	15	od540	od540	NOUN
brj-24573	57	16	)	)	PUNCT
brj-24573	57	17	values	value	NOUN
brj-24573	57	18	during	during	ADP
brj-24573	57	19	the	the	DET
brj-24573	57	20	growth	growth	NOUN
brj-24573	57	21	of	of	ADP
brj-24573	57	22	the	the	DET
brj-24573	57	23	cultures	culture	NOUN
brj-24573	57	24	,	,	PUNCT
brj-24573	57	25	spanning	span	VERB
brj-24573	57	26	from	from	ADP
brj-24573	57	27	0.1	0.1	NUM
brj-24573	57	28	to	to	ADP
brj-24573	57	29	1.0	1.0	NUM
brj-24573	57	30	.	.	PUNCT
brj-24573	58	1	cells	cell	NOUN
brj-24573	58	2	were	be	AUX
brj-24573	58	3	allowed	allow	VERB
brj-24573	58	4	to	to	PART
brj-24573	58	5	grow	grow	VERB
brj-24573	58	6	for	for	ADP
brj-24573	58	7	period	period	NOUN
brj-24573	58	8	of	of	ADP
brj-24573	58	9	5	5	NUM
brj-24573	58	10	-	-	SYM
brj-24573	58	11	6	6	NUM
brj-24573	58	12	h	h	NOUN
brj-24573	58	13	and	and	CCONJ
brj-24573	58	14	expression	expression	NOUN
brj-24573	58	15	level	level	NOUN
brj-24573	58	16	was	be	AUX
brj-24573	58	17	analyzed	analyze	VERB
brj-24573	58	18	on	on	ADP
brj-24573	58	19	sds	sds	PROPN
brj-24573	58	20	page	page	NOUN
brj-24573	58	21	.	.	PUNCT
brj-24573	59	1	further	further	ADJ
brj-24573	59	2	experiments	experiment	NOUN
brj-24573	59	3	were	be	AUX
brj-24573	59	4	conducted	conduct	VERB
brj-24573	59	5	to	to	PART
brj-24573	59	6	optimize	optimize	VERB
brj-24573	59	7	the	the	DET
brj-24573	59	8	inducer	inducer	NOUN
brj-24573	59	9	concentration	concentration	NOUN
brj-24573	59	10	.	.	PUNCT
brj-24573	60	1	the	the	DET
brj-24573	60	2	100	100	NUM
brj-24573	60	3	-	-	ADJ
brj-24573	60	4	fold	fold	ADJ
brj-24573	60	5	diluted	dilute	VERB
brj-24573	60	6	cultures	culture	NOUN
brj-24573	60	7	were	be	AUX
brj-24573	60	8	divided	divide	VERB
brj-24573	60	9	into	into	ADP
brj-24573	60	10	equal	equal	ADJ
brj-24573	60	11	portions	portion	NOUN
brj-24573	60	12	and	and	CCONJ
brj-24573	60	13	supplemented	supplement	VERB
brj-24573	60	14	with	with	ADP
brj-24573	60	15	different	different	ADJ
brj-24573	60	16	amounts	amount	NOUN
brj-24573	60	17	of	of	ADP
brj-24573	60	18	xylose	xylose	PROPN
brj-24573	60	19	,	,	PUNCT
brj-24573	60	20	ranging	range	VERB
brj-24573	60	21	from	from	ADP
brj-24573	60	22	0.01	0.01	NUM
brj-24573	60	23	%	%	NOUN
brj-24573	60	24	to	to	ADP
brj-24573	60	25	2.5	2.5	NUM
brj-24573	60	26	%	%	NOUN
brj-24573	60	27	.	.	PUNCT
brj-24573	61	1	these	these	DET
brj-24573	61	2	cultures	culture	NOUN
brj-24573	61	3	were	be	AUX
brj-24573	61	4	grown	grow	VERB
brj-24573	61	5	for	for	ADP
brj-24573	61	6	5	5	NUM
brj-24573	61	7	h	h	NOUN
brj-24573	61	8	,	,	PUNCT
brj-24573	61	9	and	and	CCONJ
brj-24573	61	10	the	the	DET
brj-24573	61	11	amount	amount	NOUN
brj-24573	61	12	of	of	ADP
brj-24573	61	13	secreted	secrete	VERB
brj-24573	61	14	amytv	amytv	NOUN
brj-24573	61	15	was	be	AUX
brj-24573	61	16	measured	measure	VERB
brj-24573	61	17	using	use	VERB
brj-24573	61	18	amylase	amylase	NOUN
brj-24573	61	19	activity	activity	NOUN
brj-24573	61	20	assay	assay	VERB
brj-24573	61	21	.	.	PUNCT
brj-24573	62	1	analysis	analysis	NOUN
brj-24573	62	2	of	of	ADP
brj-24573	62	3	catabolite	catabolite	ADJ
brj-24573	62	4	repression	repression	NOUN
brj-24573	62	5	in	in	ADP
brj-24573	62	6	order	order	NOUN
brj-24573	62	7	to	to	PART
brj-24573	62	8	analyze	analyze	VERB
brj-24573	62	9	how	how	SCONJ
brj-24573	62	10	glucose	glucose	NOUN
brj-24573	62	11	affects	affect	VERB
brj-24573	62	12	the	the	DET
brj-24573	62	13	reversal	reversal	NOUN
brj-24573	62	14	of	of	ADP
brj-24573	62	15	xylose	xylose	PROPN
brj-24573	62	16	induction	induction	NOUN
brj-24573	62	17	in	in	ADP
brj-24573	62	18	the	the	DET
brj-24573	62	19	synthesis	synthesis	NOUN
brj-24573	62	20	of	of	ADP
brj-24573	62	21	amytv	amytv	PROPN
brj-24573	62	22	,	,	PUNCT
brj-24573	62	23	an	an	DET
brj-24573	62	24	overnight	overnight	ADJ
brj-24573	62	25	grown	grow	VERB
brj-24573	62	26	culture	culture	NOUN
brj-24573	62	27	of	of	ADP
brj-24573	62	28	xyla	xyla	PROPN
brj-24573	62	29	-	-	PUNCT
brj-24573	62	30	amy(tv	amy(tv	VERB
brj-24573	62	31	)	)	PUNCT
brj-24573	62	32	was	be	AUX
brj-24573	62	33	diluted	dilute	VERB
brj-24573	62	34	100	100	NUM
brj-24573	62	35	-	-	NUM
brj-24573	62	36	fold	fold	ADJ
brj-24573	62	37	.	.	PUNCT
brj-24573	63	1	this	this	DET
brj-24573	63	2	culture	culture	NOUN
brj-24573	63	3	was	be	AUX
brj-24573	63	4	then	then	ADV
brj-24573	63	5	divided	divide	VERB
brj-24573	63	6	into	into	ADP
brj-24573	63	7	three	three	NUM
brj-24573	63	8	portions	portion	NOUN
brj-24573	63	9	:	:	PUNCT
brj-24573	63	10	one	one	NUM
brj-24573	63	11	without	without	ADP
brj-24573	63	12	any	any	DET
brj-24573	63	13	sugar	sugar	NOUN
brj-24573	63	14	supplementation	supplementation	NOUN
brj-24573	63	15	,	,	PUNCT
brj-24573	63	16	and	and	CCONJ
brj-24573	63	17	the	the	DET
brj-24573	63	18	other	other	ADJ
brj-24573	63	19	two	two	NUM
brj-24573	63	20	with	with	ADP
brj-24573	63	21	the	the	DET
brj-24573	63	22	addition	addition	NOUN
brj-24573	63	23	of	of	ADP
brj-24573	63	24	xylose	xylose	PROPN
brj-24573	63	25	(	(	PUNCT
brj-24573	63	26	2	2	NUM
brj-24573	63	27	%	%	NOUN
brj-24573	63	28	)	)	PUNCT
brj-24573	63	29	and	and	CCONJ
brj-24573	63	30	glucose	glucose	NOUN
brj-24573	63	31	(	(	PUNCT
brj-24573	63	32	2	2	NUM
brj-24573	63	33	%	%	NOUN
brj-24573	63	34	)	)	PUNCT
brj-24573	63	35	,	,	PUNCT
brj-24573	63	36	respectively	respectively	ADV
brj-24573	63	37	.	.	PUNCT
brj-24573	64	1	after	after	ADP
brj-24573	64	2	1	1	NUM
brj-24573	64	3	,	,	PUNCT
brj-24573	64	4	2	2	NUM
brj-24573	64	5	,	,	PUNCT
brj-24573	64	6	3	3	NUM
brj-24573	64	7	,	,	PUNCT
brj-24573	64	8	and	and	CCONJ
brj-24573	64	9	5	5	NUM
brj-24573	64	10	h	h	NOUN
brj-24573	64	11	,	,	PUNCT
brj-24573	64	12	small	small	ADJ
brj-24573	64	13	samples	sample	NOUN
brj-24573	64	14	(	(	PUNCT
brj-24573	64	15	aliquots	aliquot	NOUN
brj-24573	64	16	)	)	PUNCT
brj-24573	64	17	were	be	AUX
brj-24573	64	18	taken	take	VERB
brj-24573	64	19	from	from	ADP
brj-24573	64	20	each	each	DET
brj-24573	64	21	portion	portion	NOUN
brj-24573	64	22	.	.	PUNCT
brj-24573	65	1	then	then	ADV
brj-24573	65	2	,	,	PUNCT
brj-24573	65	3	2	2	NUM
brj-24573	65	4	%	%	NOUN
brj-24573	65	5	glucose	glucose	NOUN
brj-24573	65	6	was	be	AUX
brj-24573	65	7	added	add	VERB
brj-24573	65	8	to	to	ADP
brj-24573	65	9	each	each	DET
brj-24573	65	10	portion	portion	NOUN
brj-24573	65	11	,	,	PUNCT
brj-24573	65	12	and	and	CCONJ
brj-24573	65	13	the	the	DET
brj-24573	65	14	cultures	culture	NOUN
brj-24573	65	15	were	be	AUX
brj-24573	65	16	incubated	incubate	VERB
brj-24573	65	17	for	for	ADP
brj-24573	65	18	an	an	DET
brj-24573	65	19	additional	additional	ADJ
brj-24573	65	20	5	5	NUM
brj-24573	65	21	h.	h.	NOUN
brj-24573	65	22	amylase	amylase	NOUN
brj-24573	65	23	activity	activity	NOUN
brj-24573	65	24	of	of	ADP
brj-24573	65	25	each	each	DET
brj-24573	65	26	fraction	fraction	NOUN
brj-24573	65	27	was	be	AUX
brj-24573	65	28	analyzed	analyze	VERB
brj-24573	65	29	using	use	VERB
brj-24573	65	30	amylase	amylase	NOUN
brj-24573	65	31	activity	activity	NOUN
brj-24573	65	32	assay	assay	VERB
brj-24573	65	33	.	.	PUNCT
brj-24573	66	1	α	α	X
brj-24573	66	2	-	-	PUNCT
brj-24573	66	3	amylase	amylase	NOUN
brj-24573	66	4	secretion	secretion	NOUN
brj-24573	66	5	to	to	PART
brj-24573	66	6	analyze	analyze	VERB
brj-24573	66	7	the	the	DET
brj-24573	66	8	secretion	secretion	NOUN
brj-24573	66	9	of	of	ADP
brj-24573	66	10	amytv	amytv	NOUN
brj-24573	66	11	under	under	ADP
brj-24573	66	12	the	the	DET
brj-24573	66	13	conditions	condition	NOUN
brj-24573	66	14	of	of	ADP
brj-24573	66	15	inducible	inducible	ADJ
brj-24573	66	16	synthesis	synthesis	NOUN
brj-24573	66	17	,	,	PUNCT
brj-24573	66	18	an	an	DET
brj-24573	66	19	overnight	overnight	ADJ
brj-24573	66	20	grown	grow	VERB
brj-24573	66	21	culture	culture	NOUN
brj-24573	66	22	of	of	ADP
brj-24573	66	23	xyla	xyla	PROPN
brj-24573	66	24	-	-	PUNCT
brj-24573	66	25	amy(tv	amy(tv	VERB
brj-24573	66	26	)	)	PUNCT
brj-24573	66	27	in	in	ADP
brj-24573	66	28	b.	b.	PROPN
brj-24573	66	29	subtilis	subtilis	PROPN
brj-24573	66	30	was	be	AUX
brj-24573	66	31	100	100	NUM
brj-24573	66	32	-	-	ADJ
brj-24573	66	33	fold	fold	ADV
brj-24573	66	34	diluted	dilute	VERB
brj-24573	66	35	and	and	CCONJ
brj-24573	66	36	the	the	DET
brj-24573	66	37	culture	culture	NOUN
brj-24573	66	38	was	be	AUX
brj-24573	66	39	further	far	ADV
brj-24573	66	40	grown	grow	VERB
brj-24573	66	41	to	to	ADP
brj-24573	66	42	od540	od540	PROPN
brj-24573	66	43	0.5	0.5	NUM
brj-24573	66	44	.	.	PUNCT
brj-24573	67	1	at	at	ADP
brj-24573	67	2	this	this	DET
brj-24573	67	3	time	time	NOUN
brj-24573	67	4	,	,	PUNCT
brj-24573	67	5	xylose	xylose	PROPN
brj-24573	67	6	(	(	PUNCT
brj-24573	67	7	2	2	NUM
brj-24573	67	8	%	%	NOUN
brj-24573	67	9	)	)	PUNCT
brj-24573	67	10	was	be	AUX
brj-24573	67	11	added	add	VERB
brj-24573	67	12	to	to	ADP
brj-24573	67	13	the	the	DET
brj-24573	67	14	growth	growth	NOUN
brj-24573	67	15	medium	medium	NOUN
brj-24573	67	16	of	of	ADP
brj-24573	67	17	one	one	NUM
brj-24573	67	18	portion	portion	NOUN
brj-24573	67	19	and	and	CCONJ
brj-24573	67	20	the	the	DET
brj-24573	67	21	other	other	ADJ
brj-24573	67	22	portion	portion	NOUN
brj-24573	67	23	was	be	AUX
brj-24573	67	24	used	use	VERB
brj-24573	67	25	as	as	ADP
brj-24573	67	26	a	a	DET
brj-24573	67	27	non	non	ADJ
brj-24573	67	28	-	-	ADJ
brj-24573	67	29	induced	induced	ADJ
brj-24573	67	30	control	control	NOUN
brj-24573	67	31	.	.	PUNCT
brj-24573	68	1	one	one	NUM
brj-24573	68	2	milliliter	milliliter	NOUN
brj-24573	68	3	fraction	fraction	NOUN
brj-24573	68	4	were	be	AUX
brj-24573	68	5	collected	collect	VERB
brj-24573	68	6	after	after	ADP
brj-24573	68	7	30	30	NUM
brj-24573	68	8	,	,	PUNCT
brj-24573	68	9	60	60	NUM
brj-24573	68	10	,	,	PUNCT
brj-24573	68	11	and	and	CCONJ
brj-24573	68	12	90	90	NUM
brj-24573	68	13	min	min	NOUN
brj-24573	68	14	of	of	ADP
brj-24573	68	15	growth	growth	NOUN
brj-24573	68	16	.	.	PUNCT
brj-24573	69	1	this	this	PRON
brj-24573	69	2	was	be	AUX
brj-24573	69	3	followed	follow	VERB
brj-24573	69	4	by	by	ADP
brj-24573	69	5	centrifugation	centrifugation	NOUN
brj-24573	69	6	of	of	ADP
brj-24573	69	7	the	the	DET
brj-24573	69	8	samples	sample	NOUN
brj-24573	69	9	,	,	PUNCT
brj-24573	69	10	and	and	CCONJ
brj-24573	69	11	then	then	ADV
brj-24573	69	12	cells	cell	NOUN
brj-24573	69	13	and	and	CCONJ
brj-24573	69	14	the	the	DET
brj-24573	69	15	supernatants	supernatant	NOUN
brj-24573	69	16	(	(	PUNCT
brj-24573	69	17	extracellular	extracellular	ADJ
brj-24573	69	18	fraction	fraction	NOUN
brj-24573	69	19	)	)	PUNCT
brj-24573	69	20	were	be	AUX
brj-24573	69	21	collected	collect	VERB
brj-24573	69	22	.	.	PUNCT
brj-24573	70	1	the	the	DET
brj-24573	70	2	cells	cell	NOUN
brj-24573	70	3	were	be	AUX
brj-24573	70	4	washed	wash	VERB
brj-24573	70	5	with	with	ADP
brj-24573	70	6	tris	tris	ADJ
brj-24573	70	7	-	-	PUNCT
brj-24573	70	8	acetate	acetate	NOUN
brj-24573	70	9	buffer	buffer	NOUN
brj-24573	70	10	(	(	PUNCT
brj-24573	70	11	10	10	NUM
brj-24573	70	12	mm	mm	PROPN
brj-24573	70	13	tris	tris	NOUN
brj-24573	70	14	-	-	PUNCT
brj-24573	70	15	acetate	acetate	NOUN
brj-24573	70	16	,	,	PUNCT
brj-24573	70	17	ph	ph	NOUN
brj-24573	70	18	7.0	7.0	NUM
brj-24573	70	19	,	,	PUNCT
brj-24573	70	20	10	10	NUM
brj-24573	70	21	mm	mm	NUM
brj-24573	70	22	cacl2	cacl2	NOUN
brj-24573	70	23	)	)	PUNCT
brj-24573	70	24	,	,	PUNCT
brj-24573	70	25	resuspended	resuspend	VERB
brj-24573	70	26	in	in	ADP
brj-24573	70	27	0.5	0.5	NUM
brj-24573	70	28	ml	ml	NOUN
brj-24573	70	29	tris	tris	ADJ
brj-24573	70	30	-	-	PUNCT
brj-24573	70	31	acetate	acetate	NOUN
brj-24573	70	32	buffer	buffer	NOUN
brj-24573	70	33	and	and	CCONJ
brj-24573	70	34	sonicated	sonicate	VERB
brj-24573	70	35	for	for	ADP
brj-24573	70	36	5	5	NUM
brj-24573	70	37	min	min	NOUN
brj-24573	70	38	.	.	PUNCT
brj-24573	71	1	the	the	DET
brj-24573	71	2	cell	cell	NOUN
brj-24573	71	3	lysates	lysate	NOUN
brj-24573	71	4	were	be	AUX
brj-24573	71	5	again	again	ADV
brj-24573	71	6	centrifuged	centrifuge	VERB
brj-24573	71	7	providing	provide	VERB
brj-24573	71	8	soluble	soluble	ADJ
brj-24573	71	9	(	(	PUNCT
brj-24573	71	10	cytosolic	cytosolic	NOUN
brj-24573	71	11	)	)	PUNCT
brj-24573	71	12	peer	peer	NOUN
brj-24573	71	13	-	-	PUNCT
brj-24573	71	14	reviewed	review	VERB
brj-24573	71	15	article	article	NOUN
brj-24573	71	16	bioresources.cnr.ncsu.edu	bioresources.cnr.ncsu.edu	X
brj-24573	71	17	farouk	farouk	PROPN
brj-24573	71	18	et	et	PROPN
brj-24573	71	19	al	al	PROPN
brj-24573	71	20	.	.	PROPN
brj-24573	72	1	(	(	PUNCT
brj-24573	72	2	2025	2025	NUM
brj-24573	72	3	)	)	PUNCT
brj-24573	72	4	.	.	PUNCT
brj-24573	73	1	“	"	PUNCT
brj-24573	73	2	amylase	amylase	NOUN
brj-24573	73	3	via	via	ADP
brj-24573	73	4	xylose	xylose	PROPN
brj-24573	73	5	promoter	promoter	NOUN
brj-24573	73	6	,	,	PUNCT
brj-24573	73	7	”	"	PUNCT
brj-24573	73	8	bioresources	bioresource	NOUN
brj-24573	73	9	20(3	20(3	NOUN
brj-24573	73	10	)	)	PUNCT
brj-24573	73	11	,	,	PUNCT
brj-24573	73	12	7728	7728	NUM
brj-24573	73	13	-	-	SYM
brj-24573	73	14	7737	7737	NUM
brj-24573	73	15	.	.	PUNCT
brj-24573	73	16	7731	7731	NUM
brj-24573	73	17	and	and	CCONJ
brj-24573	73	18	insoluble	insoluble	ADJ
brj-24573	73	19	(	(	PUNCT
brj-24573	73	20	cell	cell	NOUN
brj-24573	73	21	wall	wall	NOUN
brj-24573	73	22	)	)	PUNCT
brj-24573	73	23	fractions	fraction	NOUN
brj-24573	73	24	.	.	PUNCT
brj-24573	74	1	the	the	DET
brj-24573	74	2	insoluble	insoluble	ADJ
brj-24573	74	3	pellet	pellet	NOUN
brj-24573	74	4	fraction	fraction	NOUN
brj-24573	74	5	was	be	AUX
brj-24573	74	6	resuspended	resuspend	VERB
brj-24573	74	7	in	in	ADP
brj-24573	74	8	0.5	0.5	NUM
brj-24573	74	9	ml	ml	NOUN
brj-24573	74	10	tris	tris	ADJ
brj-24573	74	11	-	-	PUNCT
brj-24573	74	12	acetate	acetate	NOUN
brj-24573	74	13	buffer	buffer	NOUN
brj-24573	74	14	.	.	PUNCT
brj-24573	75	1	the	the	DET
brj-24573	75	2	amylase	amylase	NOUN
brj-24573	75	3	activity	activity	NOUN
brj-24573	75	4	in	in	ADP
brj-24573	75	5	the	the	DET
brj-24573	75	6	pellet	pellet	NOUN
brj-24573	75	7	,	,	PUNCT
brj-24573	75	8	cytosolic	cytosolic	ADJ
brj-24573	75	9	fraction	fraction	NOUN
brj-24573	75	10	,	,	PUNCT
brj-24573	75	11	and	and	CCONJ
brj-24573	75	12	supernatant	supernatant	NOUN
brj-24573	75	13	were	be	AUX
brj-24573	75	14	then	then	ADV
brj-24573	75	15	measured	measure	VERB
brj-24573	75	16	.	.	PUNCT
brj-24573	76	1	α	α	X
brj-24573	76	2	-	-	PUNCT
brj-24573	76	3	amylase	amylase	NOUN
brj-24573	76	4	activity	activity	NOUN
brj-24573	76	5	assay	assay	VERB
brj-24573	76	6	the	the	DET
brj-24573	76	7	enzyme	enzyme	NOUN
brj-24573	76	8	activity	activity	NOUN
brj-24573	76	9	of	of	ADP
brj-24573	76	10	recombinant	recombinant	ADJ
brj-24573	76	11	α	α	NOUN
brj-24573	76	12	-	-	NOUN
brj-24573	76	13	amylase	amylase	NOUN
brj-24573	76	14	from	from	ADP
brj-24573	76	15	b.	b.	PROPN
brj-24573	76	16	subtilis	subtilis	PROPN
brj-24573	76	17	was	be	AUX
brj-24573	76	18	determined	determine	VERB
brj-24573	76	19	using	use	VERB
brj-24573	76	20	standard	standard	ADJ
brj-24573	76	21	dinitro	dinitro	ADJ
brj-24573	76	22	-	-	PUNCT
brj-24573	76	23	salicylic	salicylic	ADJ
brj-24573	76	24	acid	acid	NOUN
brj-24573	76	25	method	method	NOUN
brj-24573	76	26	(	(	PUNCT
brj-24573	76	27	dns	dns	NOUN
brj-24573	76	28	assay	assay	PROPN
brj-24573	76	29	)	)	PUNCT
brj-24573	76	30	method	method	NOUN
brj-24573	76	31	(	(	PUNCT
brj-24573	76	32	miller	miller	PROPN
brj-24573	76	33	1959	1959	NUM
brj-24573	76	34	;	;	PUNCT
brj-24573	76	35	aroob	aroob	PROPN
brj-24573	76	36	et	et	PROPN
brj-24573	76	37	al	al	PROPN
brj-24573	76	38	.	.	PROPN
brj-24573	76	39	2022	2022	NUM
brj-24573	76	40	)	)	PUNCT
brj-24573	76	41	.	.	PUNCT
brj-24573	77	1	the	the	DET
brj-24573	77	2	amount	amount	NOUN
brj-24573	77	3	of	of	ADP
brj-24573	77	4	liberated	liberated	ADJ
brj-24573	77	5	sugars	sugar	NOUN
brj-24573	77	6	was	be	AUX
brj-24573	77	7	quantified	quantify	VERB
brj-24573	77	8	in	in	ADP
brj-24573	77	9	terms	term	NOUN
brj-24573	77	10	of	of	ADP
brj-24573	77	11	the	the	DET
brj-24573	77	12	amount	amount	NOUN
brj-24573	77	13	of	of	ADP
brj-24573	77	14	reducing	reduce	VERB
brj-24573	77	15	sugars	sugar	NOUN
brj-24573	77	16	liberated	liberate	VERB
brj-24573	77	17	upon	upon	SCONJ
brj-24573	77	18	incubation	incubation	NOUN
brj-24573	77	19	with	with	ADP
brj-24573	77	20	the	the	DET
brj-24573	77	21	substrate	substrate	NOUN
brj-24573	77	22	.	.	PUNCT
brj-24573	78	1	in	in	ADP
brj-24573	78	2	a	a	DET
brj-24573	78	3	standard	standard	ADJ
brj-24573	78	4	assay	assay	NOUN
brj-24573	78	5	mixture	mixture	NOUN
brj-24573	78	6	,	,	PUNCT
brj-24573	78	7	100	100	NUM
brj-24573	78	8	 	 	SPACE
brj-24573	78	9	μl	μl	INTJ
brj-24573	78	10	of	of	ADP
brj-24573	78	11	1	1	NUM
brj-24573	78	12	%	%	NOUN
brj-24573	78	13	(	(	PUNCT
brj-24573	78	14	w	w	NOUN
brj-24573	78	15	/	/	SYM
brj-24573	78	16	v	v	NOUN
brj-24573	78	17	)	)	PUNCT
brj-24573	78	18	starch	starch	NOUN
brj-24573	78	19	in	in	ADP
brj-24573	78	20	suitable	suitable	ADJ
brj-24573	78	21	buffer	buffer	NOUN
brj-24573	78	22	was	be	AUX
brj-24573	78	23	mixed	mix	VERB
brj-24573	78	24	with	with	ADP
brj-24573	78	25	100	100	NUM
brj-24573	78	26	 	 	SPACE
brj-24573	78	27	μl	μl	NOUN
brj-24573	78	28	of	of	ADP
brj-24573	78	29	solution	solution	NOUN
brj-24573	78	30	containing	contain	VERB
brj-24573	78	31	appropriate	appropriate	ADJ
brj-24573	78	32	amount	amount	NOUN
brj-24573	78	33	of	of	ADP
brj-24573	78	34	enzyme	enzyme	NOUN
brj-24573	78	35	followed	follow	VERB
brj-24573	78	36	by	by	ADP
brj-24573	78	37	incubation	incubation	NOUN
brj-24573	78	38	at	at	ADP
brj-24573	78	39	60	60	NUM
brj-24573	78	40	 	 	SPACE
brj-24573	78	41	°	°	PROPN
brj-24573	78	42	c	c	NOUN
brj-24573	78	43	for	for	ADP
brj-24573	78	44	20	20	NUM
brj-24573	78	45	 	 	SPACE
brj-24573	78	46	min	min	NOUN
brj-24573	78	47	.	.	PUNCT
brj-24573	79	1	the	the	DET
brj-24573	79	2	reaction	reaction	NOUN
brj-24573	79	3	was	be	AUX
brj-24573	79	4	stopped	stop	VERB
brj-24573	79	5	by	by	ADP
brj-24573	79	6	quenching	quench	VERB
brj-24573	79	7	in	in	ADP
brj-24573	79	8	ice	ice	NOUN
brj-24573	79	9	water	water	NOUN
brj-24573	79	10	,	,	PUNCT
brj-24573	79	11	and	and	CCONJ
brj-24573	79	12	the	the	DET
brj-24573	79	13	released	release	VERB
brj-24573	79	14	reducing	reduce	VERB
brj-24573	79	15	ends	end	NOUN
brj-24573	79	16	were	be	AUX
brj-24573	79	17	determined	determine	VERB
brj-24573	79	18	by	by	ADP
brj-24573	79	19	the	the	DET
brj-24573	79	20	dns	dns	NOUN
brj-24573	79	21	method	method	NOUN
brj-24573	79	22	.	.	PUNCT
brj-24573	80	1	activity	activity	NOUN
brj-24573	80	2	unit	unit	NOUN
brj-24573	80	3	was	be	AUX
brj-24573	80	4	defined	define	VERB
brj-24573	80	5	as	as	ADP
brj-24573	80	6	µmoles	µmole	NOUN
brj-24573	80	7	/	/	SYM
brj-24573	80	8	min	min	NOUN
brj-24573	80	9	/	/	SYM
brj-24573	80	10	mg	mg	PROPN
brj-24573	80	11	.	.	PUNCT
brj-24573	80	12	results	result	VERB
brj-24573	80	13	sequence	sequence	NOUN
brj-24573	80	14	analysis	analysis	NOUN
brj-24573	80	15	the	the	DET
brj-24573	80	16	amino	amino	NOUN
brj-24573	80	17	acid	acid	NOUN
brj-24573	80	18	sequence	sequence	NOUN
brj-24573	80	19	of	of	ADP
brj-24573	80	20	amytv	amytv	NOUN
brj-24573	80	21	belonging	belong	VERB
brj-24573	80	22	to	to	ADP
brj-24573	80	23	glycoside	glycoside	NOUN
brj-24573	80	24	hydrolase	hydrolase	NOUN
brj-24573	80	25	family	family	NOUN
brj-24573	80	26	13	13	NUM
brj-24573	80	27	is	be	AUX
brj-24573	80	28	available	available	ADJ
brj-24573	80	29	on	on	ADP
brj-24573	80	30	uniprot	uniprot	NOUN
brj-24573	80	31	under	under	ADP
brj-24573	80	32	accession	accession	NOUN
brj-24573	80	33	no	no	NOUN
brj-24573	80	34	.	.	PUNCT
brj-24573	81	1	q60051	q60051	PROPN
brj-24573	81	2	.	.	PUNCT
brj-24573	82	1	the	the	DET
brj-24573	82	2	gene	gene	NOUN
brj-24573	82	3	comprises	comprise	NOUN
brj-24573	82	4	of	of	ADP
brj-24573	82	5	1446	1446	NUM
brj-24573	82	6	nucleotides	nucleotide	NOUN
brj-24573	82	7	encoding	encode	VERB
brj-24573	82	8	an	an	DET
brj-24573	82	9	amino	amino	NOUN
brj-24573	82	10	acid	acid	NOUN
brj-24573	82	11	chain	chain	NOUN
brj-24573	82	12	of	of	ADP
brj-24573	82	13	482	482	NUM
brj-24573	82	14	amino	amino	ADJ
brj-24573	82	15	acids	acid	NOUN
brj-24573	82	16	.	.	PUNCT
brj-24573	83	1	the	the	DET
brj-24573	83	2	amino	amino	NOUN
brj-24573	83	3	acids	acid	NOUN
brj-24573	83	4	from	from	ADP
brj-24573	83	5	1	1	NUM
brj-24573	83	6	to	to	PART
brj-24573	83	7	29	29	NUM
brj-24573	83	8	are	be	AUX
brj-24573	83	9	predicted	predict	VERB
brj-24573	83	10	as	as	ADP
brj-24573	83	11	signal	signal	NOUN
brj-24573	83	12	sequence	sequence	NOUN
brj-24573	83	13	while	while	SCONJ
brj-24573	83	14	those	those	DET
brj-24573	83	15	from	from	ADP
brj-24573	83	16	30	30	NUM
brj-24573	83	17	to	to	PART
brj-24573	83	18	482	482	NUM
brj-24573	83	19	encode	encode	ADJ
brj-24573	83	20	α	α	NOUN
brj-24573	83	21	-	-	PUNCT
brj-24573	83	22	amylase	amylase	NOUN
brj-24573	83	23	region	region	NOUN
brj-24573	83	24	having	have	VERB
brj-24573	83	25	distinct	distinct	ADJ
brj-24573	83	26	gh13	gh13	PROPN
brj-24573	83	27	catalytic	catalytic	ADJ
brj-24573	83	28	region	region	NOUN
brj-24573	83	29	(	(	PUNCT
brj-24573	83	30	42	42	NUM
brj-24573	83	31	to	to	PART
brj-24573	83	32	393	393	NUM
brj-24573	83	33	)	)	PUNCT
brj-24573	83	34	and	and	CCONJ
brj-24573	83	35	classical	classical	ADJ
brj-24573	83	36	α	α	NOUN
brj-24573	83	37	-	-	NOUN
brj-24573	83	38	amylase	amylase	NOUN
brj-24573	83	39	c	c	NOUN
brj-24573	83	40	-	-	PUNCT
brj-24573	83	41	terminus	terminus	NOUN
brj-24573	83	42	region	region	NOUN
brj-24573	83	43	(	(	PUNCT
brj-24573	83	44	405	405	NUM
brj-24573	83	45	to	to	PART
brj-24573	83	46	482	482	NUM
brj-24573	83	47	)	)	PUNCT
brj-24573	83	48	(	(	PUNCT
brj-24573	83	49	fig	fig	NOUN
brj-24573	83	50	.	.	PUNCT
brj-24573	83	51	1b	1b	NUM
brj-24573	83	52	)	)	PUNCT
brj-24573	83	53	.	.	PUNCT
brj-24573	84	1	the	the	DET
brj-24573	84	2	upstream	upstream	ADJ
brj-24573	84	3	and	and	CCONJ
brj-24573	84	4	downstream	downstream	ADJ
brj-24573	84	5	regulatory	regulatory	ADJ
brj-24573	84	6	elements	element	NOUN
brj-24573	84	7	of	of	ADP
brj-24573	84	8	amytv	amytv	NOUN
brj-24573	84	9	have	have	AUX
brj-24573	84	10	previously	previously	ADV
brj-24573	84	11	been	be	AUX
brj-24573	84	12	studied	study	VERB
brj-24573	84	13	extensively	extensively	ADV
brj-24573	84	14	by	by	ADP
brj-24573	84	15	hofemeister	hofemeister	PROPN
brj-24573	84	16	et	et	PROPN
brj-24573	84	17	al	al	PROPN
brj-24573	84	18	.	.	PROPN
brj-24573	85	1	(	(	PUNCT
brj-24573	85	2	1994	1994	NUM
brj-24573	85	3	)	)	PUNCT
brj-24573	85	4	.	.	PUNCT
brj-24573	86	1	fig	fig	NOUN
brj-24573	86	2	.	.	PUNCT
brj-24573	87	1	1	1	X
brj-24573	87	2	.	.	X
brj-24573	87	3	(	(	PUNCT
brj-24573	87	4	a	a	X
brj-24573	87	5	)	)	PUNCT
brj-24573	87	6	structure	structure	NOUN
brj-24573	87	7	of	of	ADP
brj-24573	87	8	the	the	DET
brj-24573	87	9	b.	b.	PROPN
brj-24573	87	10	subtilis	subtilis	PROPN
brj-24573	87	11	expression	expression	NOUN
brj-24573	87	12	construct	construct	VERB
brj-24573	87	13	xyl	xyl	PROPN
brj-24573	87	14	-	-	PUNCT
brj-24573	87	15	amy(tv	amy(tv	VERB
brj-24573	87	16	)	)	PUNCT
brj-24573	87	17	containing	contain	VERB
brj-24573	87	18	the	the	DET
brj-24573	87	19	glucose	glucose	NOUN
brj-24573	87	20	box	box	NOUN
brj-24573	87	21	(	(	PUNCT
brj-24573	87	22	g.	g.	PROPN
brj-24573	87	23	box	box	PROPN
brj-24573	87	24	)	)	PUNCT
brj-24573	87	25	for	for	ADP
brj-24573	87	26	studying	study	VERB
brj-24573	87	27	the	the	DET
brj-24573	87	28	inducible	inducible	ADJ
brj-24573	87	29	expression	expression	NOUN
brj-24573	87	30	of	of	ADP
brj-24573	87	31	α	α	NOUN
brj-24573	87	32	-	-	NOUN
brj-24573	87	33	amylase	amylase	NOUN
brj-24573	87	34	in	in	ADP
brj-24573	87	35	b.	b.	PROPN
brj-24573	87	36	subtilis	subtilis	PROPN
brj-24573	87	37	;	;	PUNCT
brj-24573	87	38	amytv	amytv	PROPN
brj-24573	87	39	,	,	PUNCT
brj-24573	87	40	the	the	DET
brj-24573	87	41	promoterless	promoterless	NOUN
brj-24573	87	42	α	α	NOUN
brj-24573	87	43	-	-	NOUN
brj-24573	87	44	amylase	amylase	NOUN
brj-24573	87	45	from	from	ADP
brj-24573	87	46	t.	t.	PROPN
brj-24573	87	47	vulgaris	vulgaris	NOUN
brj-24573	87	48	;	;	PUNCT
brj-24573	87	49	xylr	xylr	PROPN
brj-24573	87	50	,	,	PUNCT
brj-24573	87	51	repressor	repressor	NOUN
brj-24573	87	52	protein	protein	NOUN
brj-24573	87	53	;	;	PUNCT
brj-24573	87	54	pxyla	pxyla	NOUN
brj-24573	87	55	,	,	PUNCT
brj-24573	87	56	the	the	DET
brj-24573	87	57	inducible	inducible	ADJ
brj-24573	87	58	xylose	xylose	PROPN
brj-24573	87	59	isomerase	isomerase	ADJ
brj-24573	87	60	promoter	promoter	NOUN
brj-24573	87	61	of	of	ADP
brj-24573	87	62	b.	b.	PROPN
brj-24573	87	63	megaterium	megaterium	PROPN
brj-24573	87	64	;	;	PUNCT
brj-24573	87	65	(	(	PUNCT
brj-24573	87	66	b	b	X
brj-24573	87	67	)	)	PUNCT
brj-24573	87	68	graphical	graphical	ADJ
brj-24573	87	69	representation	representation	NOUN
brj-24573	87	70	indicating	indicate	VERB
brj-24573	87	71	presence	presence	NOUN
brj-24573	87	72	of	of	ADP
brj-24573	87	73	signal	signal	ADJ
brj-24573	87	74	sequence	sequence	NOUN
brj-24573	87	75	region	region	NOUN
brj-24573	87	76	,	,	PUNCT
brj-24573	87	77	gh13	gh13	PROPN
brj-24573	87	78	catalytic	catalytic	ADJ
brj-24573	87	79	region	region	NOUN
brj-24573	87	80	and	and	CCONJ
brj-24573	87	81	α	α	NOUN
brj-24573	87	82	-	-	NOUN
brj-24573	87	83	amylase	amylase	NOUN
brj-24573	87	84	c	c	NOUN
brj-24573	87	85	-	-	PUNCT
brj-24573	87	86	terminus	terminus	NOUN
brj-24573	87	87	region	region	NOUN
brj-24573	87	88	in	in	ADP
brj-24573	87	89	amytv	amytv	NOUN
brj-24573	87	90	construction	construction	NOUN
brj-24573	87	91	of	of	ADP
brj-24573	87	92	xyl	xyl	PROPN
brj-24573	87	93	-	-	PUNCT
brj-24573	87	94	amy(tv	amy(tv	VERB
brj-24573	87	95	)	)	PUNCT
brj-24573	87	96	cassette	cassette	NOUN
brj-24573	87	97	and	and	CCONJ
brj-24573	87	98	expression	expression	NOUN
brj-24573	87	99	analysis	analysis	NOUN
brj-24573	87	100	the	the	DET
brj-24573	87	101	dna	dna	PROPN
brj-24573	87	102	fragment	fragment	NOUN
brj-24573	87	103	of	of	ADP
brj-24573	87	104	amytv	amytv	NOUN
brj-24573	87	105	was	be	AUX
brj-24573	87	106	ligated	ligate	VERB
brj-24573	87	107	between	between	ADP
brj-24573	87	108	msti	msti	NOUN
brj-24573	87	109	and	and	CCONJ
brj-24573	87	110	hindiii	hindiii	ADJ
brj-24573	87	111	fragments	fragment	NOUN
brj-24573	87	112	into	into	ADP
brj-24573	87	113	the	the	DET
brj-24573	87	114	sma	sma	NOUN
brj-24573	87	115	i	i	PRON
brj-24573	87	116	linearized	linearize	VERB
brj-24573	87	117	b.	b.	PROPN
brj-24573	87	118	megaterium	megaterium	NOUN
brj-24573	87	119	vector	vector	NOUN
brj-24573	87	120	pwh1520	pwh1520	NOUN
brj-24573	87	121	.	.	PUNCT
brj-24573	88	1	this	this	DET
brj-24573	88	2	dna	dna	PROPN
brj-24573	88	3	fragment	fragment	NOUN
brj-24573	88	4	laid	lay	VERB
brj-24573	88	5	downstream	downstream	NOUN
brj-24573	88	6	of	of	ADP
brj-24573	88	7	the	the	DET
brj-24573	88	8	glucose	glucose	NOUN
brj-24573	88	9	box	box	NOUN
brj-24573	88	10	of	of	ADP
brj-24573	88	11	the	the	DET
brj-24573	88	12	pxyla	pxyla	NOUN
brj-24573	88	13	-	-	PUNCT
brj-24573	88	14	promotor	promotor	NOUN
brj-24573	88	15	.	.	PUNCT
brj-24573	89	1	the	the	DET
brj-24573	89	2	construct	construct	NOUN
brj-24573	89	3	was	be	AUX
brj-24573	89	4	named	name	VERB
brj-24573	89	5	xylaamy(tv	xylaamy(tv	PROPN
brj-24573	89	6	)	)	PUNCT
brj-24573	89	7	(	(	PUNCT
brj-24573	89	8	fig	fig	NOUN
brj-24573	89	9	.	.	PUNCT
brj-24573	90	1	1a	1a	NOUN
brj-24573	90	2	)	)	PUNCT
brj-24573	90	3	.	.	PUNCT
brj-24573	91	1	the	the	DET
brj-24573	91	2	expression	expression	NOUN
brj-24573	91	3	of	of	ADP
brj-24573	91	4	the	the	DET
brj-24573	91	5	xyla	xyla	NOUN
brj-24573	91	6	-	-	PUNCT
brj-24573	91	7	amy(tv	amy(tv	VERB
brj-24573	91	8	)	)	PUNCT
brj-24573	91	9	in	in	ADP
brj-24573	91	10	bacillus	bacillus	NOUN
brj-24573	91	11	(	(	PUNCT
brj-24573	91	12	gsb26	gsb26	NOUN
brj-24573	91	13	)	)	PUNCT
brj-24573	91	14	was	be	AUX
brj-24573	91	15	obtained	obtain	VERB
brj-24573	91	16	using	use	VERB
brj-24573	91	17	1	1	NUM
brj-24573	91	18	%	%	NOUN
brj-24573	91	19	xylose	xylose	PROPN
brj-24573	91	20	as	as	ADP
brj-24573	91	21	the	the	DET
brj-24573	91	22	inducer	inducer	NOUN
brj-24573	91	23	.	.	PUNCT
brj-24573	92	1	the	the	DET
brj-24573	92	2	protein	protein	NOUN
brj-24573	92	3	was	be	AUX
brj-24573	92	4	found	find	VERB
brj-24573	92	5	to	to	PART
brj-24573	92	6	be	be	AUX
brj-24573	92	7	produced	produce	VERB
brj-24573	92	8	in	in	ADP
brj-24573	92	9	the	the	DET
brj-24573	92	10	extracellular	extracellular	ADJ
brj-24573	92	11	fraction	fraction	NOUN
brj-24573	92	12	(	(	PUNCT
brj-24573	92	13	fig	fig	NOUN
brj-24573	92	14	.	.	PUNCT
brj-24573	93	1	2	2	NUM
brj-24573	93	2	)	)	PUNCT
brj-24573	93	3	.	.	PUNCT
brj-24573	94	1	parallel	parallel	ADJ
brj-24573	94	2	analysis	analysis	NOUN
brj-24573	94	3	of	of	ADP
brj-24573	94	4	uninduced	uninduced	ADJ
brj-24573	94	5	samples	sample	NOUN
brj-24573	94	6	,	,	PUNCT
brj-24573	94	7	without	without	ADP
brj-24573	94	8	the	the	DET
brj-24573	94	9	addition	addition	NOUN
brj-24573	94	10	of	of	ADP
brj-24573	94	11	xylose	xylose	PROPN
brj-24573	94	12	,	,	PUNCT
brj-24573	94	13	indicated	indicate	VERB
brj-24573	94	14	absence	absence	NOUN
brj-24573	94	15	of	of	ADP
brj-24573	94	16	amylase	amylase	NOUN
brj-24573	94	17	in	in	ADP
brj-24573	94	18	the	the	DET
brj-24573	94	19	extracellular	extracellular	ADJ
brj-24573	94	20	fraction	fraction	NOUN
brj-24573	94	21	.	.	PUNCT
brj-24573	95	1	peer	peer	NOUN
brj-24573	95	2	-	-	PUNCT
brj-24573	95	3	reviewed	review	VERB
brj-24573	95	4	article	article	NOUN
brj-24573	95	5	bioresources.cnr.ncsu.edu	bioresources.cnr.ncsu.edu	X
brj-24573	95	6	farouk	farouk	PROPN
brj-24573	95	7	et	et	PROPN
brj-24573	95	8	al	al	PROPN
brj-24573	95	9	.	.	PROPN
brj-24573	96	1	(	(	PUNCT
brj-24573	96	2	2025	2025	NUM
brj-24573	96	3	)	)	PUNCT
brj-24573	96	4	.	.	PUNCT
brj-24573	97	1	“	"	PUNCT
brj-24573	97	2	amylase	amylase	NOUN
brj-24573	97	3	via	via	ADP
brj-24573	97	4	xylose	xylose	PROPN
brj-24573	97	5	promoter	promoter	NOUN
brj-24573	97	6	,	,	PUNCT
brj-24573	97	7	”	"	PUNCT
brj-24573	97	8	bioresources	bioresource	NOUN
brj-24573	97	9	20(3	20(3	NOUN
brj-24573	97	10	)	)	PUNCT
brj-24573	97	11	,	,	PUNCT
brj-24573	97	12	7728	7728	NUM
brj-24573	97	13	-	-	SYM
brj-24573	97	14	7737	7737	NUM
brj-24573	97	15	.	.	PUNCT
brj-24573	98	1	7732	7732	NUM
brj-24573	98	2	optimization	optimization	NOUN
brj-24573	98	3	of	of	ADP
brj-24573	98	4	inducer	inducer	NOUN
brj-24573	98	5	concentration	concentration	NOUN
brj-24573	98	6	and	and	CCONJ
brj-24573	98	7	α	α	NOUN
brj-24573	98	8	-	-	PUNCT
brj-24573	98	9	amylase	amylase	NOUN
brj-24573	98	10	secretion	secretion	NOUN
brj-24573	98	11	optimization	optimization	NOUN
brj-24573	98	12	studies	study	NOUN
brj-24573	98	13	on	on	ADP
brj-24573	98	14	inducer	inducer	NOUN
brj-24573	98	15	concentration	concentration	NOUN
brj-24573	98	16	showed	show	VERB
brj-24573	98	17	that	that	SCONJ
brj-24573	98	18	adding	add	VERB
brj-24573	98	19	the	the	DET
brj-24573	98	20	inducer	inducer	NOUN
brj-24573	98	21	during	during	ADP
brj-24573	98	22	the	the	DET
brj-24573	98	23	early	early	ADJ
brj-24573	98	24	logarithmic	logarithmic	ADJ
brj-24573	98	25	phase	phase	NOUN
brj-24573	98	26	of	of	ADP
brj-24573	98	27	the	the	DET
brj-24573	98	28	culture	culture	NOUN
brj-24573	98	29	gave	give	VERB
brj-24573	98	30	the	the	DET
brj-24573	98	31	most	most	ADV
brj-24573	98	32	effective	effective	ADJ
brj-24573	98	33	results	result	NOUN
brj-24573	98	34	for	for	ADP
brj-24573	98	35	optimal	optimal	ADJ
brj-24573	98	36	induction	induction	NOUN
brj-24573	98	37	.	.	PUNCT
brj-24573	99	1	furthermore	furthermore	ADV
brj-24573	99	2	,	,	PUNCT
brj-24573	99	3	maximum	maximum	ADJ
brj-24573	99	4	α	α	PRON
brj-24573	99	5	-	-	PUNCT
brj-24573	99	6	amylase	amylase	NOUN
brj-24573	99	7	activity	activity	NOUN
brj-24573	99	8	was	be	AUX
brj-24573	99	9	observed	observe	VERB
brj-24573	99	10	when	when	SCONJ
brj-24573	99	11	the	the	DET
brj-24573	99	12	cultures	culture	NOUN
brj-24573	99	13	were	be	AUX
brj-24573	99	14	induced	induce	VERB
brj-24573	99	15	with	with	ADP
brj-24573	99	16	2	2	NUM
brj-24573	99	17	%	%	NOUN
brj-24573	99	18	xylose	xylose	NOUN
brj-24573	99	19	(	(	PUNCT
brj-24573	99	20	fig	fig	NOUN
brj-24573	99	21	.	.	PUNCT
brj-24573	100	1	3	3	NUM
brj-24573	100	2	)	)	PUNCT
brj-24573	100	3	.	.	PUNCT
brj-24573	101	1	therefore	therefore	ADV
brj-24573	101	2	,	,	PUNCT
brj-24573	101	3	for	for	ADP
brj-24573	101	4	the	the	DET
brj-24573	101	5	subsequent	subsequent	ADJ
brj-24573	101	6	induction	induction	NOUN
brj-24573	101	7	experiments	experiment	NOUN
brj-24573	101	8	,	,	PUNCT
brj-24573	101	9	a	a	DET
brj-24573	101	10	xylose	xylose	ADJ
brj-24573	101	11	concentration	concentration	NOUN
brj-24573	101	12	of	of	ADP
brj-24573	101	13	2	2	NUM
brj-24573	101	14	%	%	NOUN
brj-24573	101	15	was	be	AUX
brj-24573	101	16	considered	consider	VERB
brj-24573	101	17	optimal	optimal	ADJ
brj-24573	101	18	.	.	PUNCT
brj-24573	102	1	fig	fig	NOUN
brj-24573	102	2	.	.	PUNCT
brj-24573	103	1	2	2	X
brj-24573	103	2	.	.	X
brj-24573	103	3	expression	expression	NOUN
brj-24573	103	4	analysis	analysis	NOUN
brj-24573	103	5	of	of	ADP
brj-24573	103	6	amytv	amytv	NOUN
brj-24573	103	7	in	in	ADP
brj-24573	103	8	extracellular	extracellular	ADJ
brj-24573	103	9	fraction	fraction	NOUN
brj-24573	103	10	of	of	ADP
brj-24573	103	11	b.	b.	PROPN
brj-24573	103	12	subtilis	subtilis	PROPN
brj-24573	103	13	(	(	PUNCT
brj-24573	103	14	gsb26	gsb26	NOUN
brj-24573	103	15	)	)	PUNCT
brj-24573	103	16	fig	fig	NOUN
brj-24573	103	17	.	.	PUNCT
brj-24573	104	1	3	3	X
brj-24573	104	2	.	.	X
brj-24573	104	3	estimation	estimation	NOUN
brj-24573	104	4	of	of	ADP
brj-24573	104	5	the	the	DET
brj-24573	104	6	optimum	optimum	ADJ
brj-24573	104	7	d	d	NOUN
brj-24573	104	8	-	-	PUNCT
brj-24573	104	9	xylose	xylose	PROPN
brj-24573	104	10	(	(	PUNCT
brj-24573	104	11	inducer	inducer	NOUN
brj-24573	104	12	)	)	PUNCT
brj-24573	104	13	concentration	concentration	NOUN
brj-24573	104	14	for	for	ADP
brj-24573	104	15	induction	induction	NOUN
brj-24573	104	16	of	of	ADP
brj-24573	104	17	amytv	amytv	PROPN
brj-24573	104	18	secretion	secretion	NOUN
brj-24573	104	19	in	in	ADP
brj-24573	104	20	b.	b.	PROPN
brj-24573	104	21	subtilis	subtilis	PROPN
brj-24573	104	22	.	.	PUNCT
brj-24573	105	1	the	the	DET
brj-24573	105	2	concentration	concentration	NOUN
brj-24573	105	3	of	of	ADP
brj-24573	105	4	d	d	X
brj-24573	105	5	-	-	PUNCT
brj-24573	105	6	xylose	xylose	PROPN
brj-24573	105	7	was	be	AUX
brj-24573	105	8	0.01	0.01	NUM
brj-24573	105	9	%	%	NOUN
brj-24573	105	10	to	to	ADP
brj-24573	105	11	2.0	2.0	NUM
brj-24573	105	12	%	%	NOUN
brj-24573	105	13	and	and	CCONJ
brj-24573	105	14	amytv	amytv	NOUN
brj-24573	105	15	activity	activity	NOUN
brj-24573	105	16	was	be	AUX
brj-24573	105	17	estimated	estimate	VERB
brj-24573	105	18	after	after	ADP
brj-24573	105	19	5	5	NUM
brj-24573	105	20	h	h	NOUN
brj-24573	105	21	from	from	ADP
brj-24573	105	22	adding	add	VERB
brj-24573	105	23	the	the	DET
brj-24573	105	24	inducer	inducer	NOUN
brj-24573	105	25	.	.	PUNCT
brj-24573	106	1	peer	peer	NOUN
brj-24573	106	2	-	-	PUNCT
brj-24573	106	3	reviewed	review	VERB
brj-24573	106	4	article	article	NOUN
brj-24573	106	5	bioresources.cnr.ncsu.edu	bioresources.cnr.ncsu.edu	X
brj-24573	106	6	farouk	farouk	PROPN
brj-24573	106	7	et	et	PROPN
brj-24573	106	8	al	al	PROPN
brj-24573	106	9	.	.	PROPN
brj-24573	107	1	(	(	PUNCT
brj-24573	107	2	2025	2025	NUM
brj-24573	107	3	)	)	PUNCT
brj-24573	107	4	.	.	PUNCT
brj-24573	108	1	“	"	PUNCT
brj-24573	108	2	amylase	amylase	NOUN
brj-24573	108	3	via	via	ADP
brj-24573	108	4	xylose	xylose	PROPN
brj-24573	108	5	promoter	promoter	NOUN
brj-24573	108	6	,	,	PUNCT
brj-24573	108	7	”	"	PUNCT
brj-24573	108	8	bioresources	bioresource	NOUN
brj-24573	108	9	20(3	20(3	NOUN
brj-24573	108	10	)	)	PUNCT
brj-24573	108	11	,	,	PUNCT
brj-24573	108	12	7728	7728	NUM
brj-24573	108	13	-	-	SYM
brj-24573	108	14	7737	7737	NUM
brj-24573	108	15	.	.	PUNCT
brj-24573	109	1	7733	7733	NUM
brj-24573	109	2	glucose	glucose	NOUN
brj-24573	109	3	repression	repression	NOUN
brj-24573	109	4	catabolite	catabolite	ADJ
brj-24573	109	5	repression	repression	NOUN
brj-24573	109	6	is	be	AUX
brj-24573	109	7	the	the	DET
brj-24573	109	8	phenomenon	phenomenon	NOUN
brj-24573	109	9	during	during	ADP
brj-24573	109	10	which	which	PRON
brj-24573	109	11	the	the	DET
brj-24573	109	12	glucose	glucose	NOUN
brj-24573	109	13	binds	bind	VERB
brj-24573	109	14	to	to	ADP
brj-24573	109	15	the	the	DET
brj-24573	109	16	operator	operator	NOUN
brj-24573	109	17	or	or	CCONJ
brj-24573	109	18	associated	associate	VERB
brj-24573	109	19	silencers	silencer	NOUN
brj-24573	109	20	to	to	PART
brj-24573	109	21	inhibit	inhibit	VERB
brj-24573	109	22	the	the	DET
brj-24573	109	23	synthesis	synthesis	NOUN
brj-24573	109	24	of	of	ADP
brj-24573	109	25	certain	certain	ADJ
brj-24573	109	26	enzymes	enzyme	NOUN
brj-24573	109	27	(	(	PUNCT
brj-24573	109	28	hueck	hueck	NOUN
brj-24573	109	29	and	and	CCONJ
brj-24573	109	30	hillen	hillen	ADJ
brj-24573	109	31	1995	1995	NUM
brj-24573	109	32	)	)	PUNCT
brj-24573	109	33	.	.	PUNCT
brj-24573	110	1	utilizing	utilize	VERB
brj-24573	110	2	catabolite	catabolite	ADJ
brj-24573	110	3	repressors	repressor	NOUN
brj-24573	110	4	to	to	ADP
brj-24573	110	5	fine	fine	ADJ
brj-24573	110	6	tune	tune	NOUN
brj-24573	110	7	the	the	DET
brj-24573	110	8	heterologous	heterologous	ADJ
brj-24573	110	9	expression	expression	NOUN
brj-24573	110	10	of	of	ADP
brj-24573	110	11	genes	gene	NOUN
brj-24573	110	12	of	of	ADP
brj-24573	110	13	interest	interest	NOUN
brj-24573	110	14	in	in	ADP
brj-24573	110	15	heterologous	heterologous	ADJ
brj-24573	110	16	systems	system	NOUN
brj-24573	110	17	is	be	AUX
brj-24573	110	18	a	a	DET
brj-24573	110	19	strategy	strategy	NOUN
brj-24573	110	20	that	that	PRON
brj-24573	110	21	has	have	AUX
brj-24573	110	22	already	already	ADV
brj-24573	110	23	been	be	AUX
brj-24573	110	24	implemented	implement	VERB
brj-24573	110	25	in	in	ADP
brj-24573	110	26	previous	previous	ADJ
brj-24573	110	27	studies	study	NOUN
brj-24573	110	28	(	(	PUNCT
brj-24573	110	29	nair	nair	NOUN
brj-24573	110	30	and	and	CCONJ
brj-24573	110	31	sarma	sarma	PROPN
brj-24573	110	32	2021	2021	NUM
brj-24573	110	33	;	;	PUNCT
brj-24573	110	34	liu	liu	PROPN
brj-24573	110	35	et	et	PROPN
brj-24573	110	36	al	al	PROPN
brj-24573	110	37	.	.	PROPN
brj-24573	110	38	2022	2022	NUM
brj-24573	110	39	)	)	PUNCT
brj-24573	110	40	.	.	PUNCT
brj-24573	111	1	in	in	ADP
brj-24573	111	2	this	this	DET
brj-24573	111	3	study	study	NOUN
brj-24573	111	4	,	,	PUNCT
brj-24573	111	5	it	it	PRON
brj-24573	111	6	was	be	AUX
brj-24573	111	7	found	find	VERB
brj-24573	111	8	that	that	SCONJ
brj-24573	111	9	the	the	DET
brj-24573	111	10	addition	addition	NOUN
brj-24573	111	11	of	of	ADP
brj-24573	111	12	2	2	NUM
brj-24573	111	13	%	%	NOUN
brj-24573	111	14	glucose	glucose	NOUN
brj-24573	111	15	resulted	result	VERB
brj-24573	111	16	in	in	ADP
brj-24573	111	17	complete	complete	ADJ
brj-24573	111	18	repression	repression	NOUN
brj-24573	111	19	of	of	ADP
brj-24573	111	20	the	the	DET
brj-24573	111	21	amytv	amytv	NOUN
brj-24573	111	22	gene	gene	NOUN
brj-24573	111	23	expression	expression	NOUN
brj-24573	111	24	.	.	PUNCT
brj-24573	112	1	in	in	ADP
brj-24573	112	2	addition	addition	NOUN
brj-24573	112	3	,	,	PUNCT
brj-24573	112	4	when	when	SCONJ
brj-24573	112	5	glucose	glucose	NOUN
brj-24573	112	6	was	be	AUX
brj-24573	112	7	added	add	VERB
brj-24573	112	8	to	to	ADP
brj-24573	112	9	a	a	DET
brj-24573	112	10	xylose	xylose	PROPN
brj-24573	112	11	induced	induce	VERB
brj-24573	112	12	culture	culture	NOUN
brj-24573	112	13	,	,	PUNCT
brj-24573	112	14	the	the	DET
brj-24573	112	15	expression	expression	NOUN
brj-24573	112	16	and	and	CCONJ
brj-24573	112	17	secretion	secretion	NOUN
brj-24573	112	18	of	of	ADP
brj-24573	112	19	α	α	NOUN
brj-24573	112	20	-	-	PUNCT
brj-24573	112	21	amylase	amylase	NOUN
brj-24573	112	22	were	be	AUX
brj-24573	112	23	both	both	PRON
brj-24573	112	24	suppressed	suppress	VERB
brj-24573	112	25	(	(	PUNCT
brj-24573	112	26	fig	fig	NOUN
brj-24573	112	27	.	.	PUNCT
brj-24573	112	28	4	4	NUM
brj-24573	112	29	)	)	PUNCT
brj-24573	112	30	.	.	PUNCT
brj-24573	113	1	fig	fig	NOUN
brj-24573	113	2	.	.	PUNCT
brj-24573	114	1	4	4	X
brj-24573	114	2	.	.	NOUN
brj-24573	114	3	amylase	amylase	NOUN
brj-24573	114	4	activity	activity	NOUN
brj-24573	114	5	was	be	AUX
brj-24573	114	6	measured	measure	VERB
brj-24573	114	7	in	in	ADP
brj-24573	114	8	the	the	DET
brj-24573	114	9	bacterial	bacterial	ADJ
brj-24573	114	10	supernatant	supernatant	NOUN
brj-24573	114	11	under	under	ADP
brj-24573	114	12	different	different	ADJ
brj-24573	114	13	conditions	condition	NOUN
brj-24573	114	14	:	:	PUNCT
brj-24573	114	15	without	without	ADP
brj-24573	114	16	an	an	DET
brj-24573	114	17	inducer	inducer	NOUN
brj-24573	114	18	,	,	PUNCT
brj-24573	114	19	in	in	ADP
brj-24573	114	20	the	the	DET
brj-24573	114	21	presence	presence	NOUN
brj-24573	114	22	of	of	ADP
brj-24573	114	23	either	either	CCONJ
brj-24573	114	24	xylose	xylose	PROPN
brj-24573	114	25	or	or	CCONJ
brj-24573	114	26	glucose	glucose	NOUN
brj-24573	114	27	as	as	ADP
brj-24573	114	28	inducers	inducer	NOUN
brj-24573	114	29	,	,	PUNCT
brj-24573	114	30	or	or	CCONJ
brj-24573	114	31	with	with	ADP
brj-24573	114	32	a	a	DET
brj-24573	114	33	combination	combination	NOUN
brj-24573	114	34	of	of	ADP
brj-24573	114	35	both	both	PRON
brj-24573	114	36	as	as	ADP
brj-24573	114	37	inducers	inducer	NOUN
brj-24573	114	38	.	.	PUNCT
brj-24573	115	1	the	the	DET
brj-24573	115	2	activity	activity	NOUN
brj-24573	115	3	assay	assay	NOUN
brj-24573	115	4	was	be	AUX
brj-24573	115	5	carried	carry	VERB
brj-24573	115	6	out	out	ADP
brj-24573	115	7	at	at	ADP
brj-24573	115	8	various	various	ADJ
brj-24573	115	9	time	time	NOUN
brj-24573	115	10	intervals	interval	NOUN
brj-24573	115	11	ranging	range	VERB
brj-24573	115	12	from	from	ADP
brj-24573	115	13	0	0	NUM
brj-24573	115	14	to	to	ADP
brj-24573	115	15	5	5	NUM
brj-24573	115	16	h.	h.	NOUN
brj-24573	115	17	file:///d:/post%20phd	file:///d:/post%20phd	NOUN
brj-24573	115	18	/	/	SYM
brj-24573	115	19	dr.%20farouk%20taif	dr.%20farouk%20taif	PROPN
brj-24573	115	20	/	/	SYM
brj-24573	115	21	manucript%201	manucript%201	PROPN
brj-24573	115	22	/	/	SYM
brj-24573	115	23	amylase	amylase	NOUN
brj-24573	115	24	/	/	SYM
brj-24573	115	25	bioresources	bioresource	NOUN
brj-24573	115	26	/	/	SYM
brj-24573	115	27	farouk%20et%20al	farouk%20et%20al	PROPN
brj-24573	115	28	..	..	PUNCT
brj-24573	115	29	doc%23_enref_6	doc%23_enref_6	ADV
brj-24573	115	30	file:///d:/post%20phd	file:///d:/post%20phd	NOUN
brj-24573	115	31	/	/	SYM
brj-24573	115	32	dr.%20farouk%20taif	dr.%20farouk%20taif	PROPN
brj-24573	115	33	/	/	SYM
brj-24573	115	34	manucript%201	manucript%201	PROPN
brj-24573	115	35	/	/	SYM
brj-24573	115	36	amylase	amylase	NOUN
brj-24573	115	37	/	/	SYM
brj-24573	115	38	bioresources	bioresource	NOUN
brj-24573	115	39	/	/	SYM
brj-24573	115	40	farouk%20et%20al	farouk%20et%20al	PROPN
brj-24573	115	41	..	..	PUNCT
brj-24573	115	42	doc%23_enref_6	doc%23_enref_6	ADV
brj-24573	115	43	file:///d:/post%20phd	file:///d:/post%20phd	NOUN
brj-24573	115	44	/	/	SYM
brj-24573	115	45	dr.%20farouk%20taif	dr.%20farouk%20taif	PROPN
brj-24573	115	46	/	/	SYM
brj-24573	115	47	manucript%201	manucript%201	PROPN
brj-24573	115	48	/	/	SYM
brj-24573	115	49	amylase	amylase	NOUN
brj-24573	115	50	/	/	SYM
brj-24573	115	51	bioresources	bioresource	NOUN
brj-24573	115	52	/	/	SYM
brj-24573	115	53	farouk%20et%20al	farouk%20et%20al	PROPN
brj-24573	115	54	..	..	PUNCT
brj-24573	115	55	doc%23_enref_12	doc%23_enref_12	PROPN
brj-24573	115	56	file:///d:/post%20phd	file:///d:/post%20phd	NOUN
brj-24573	115	57	/	/	SYM
brj-24573	115	58	dr.%20farouk%20taif	dr.%20farouk%20taif	PROPN
brj-24573	115	59	/	/	SYM
brj-24573	115	60	manucript%201	manucript%201	PROPN
brj-24573	115	61	/	/	SYM
brj-24573	115	62	amylase	amylase	NOUN
brj-24573	115	63	/	/	SYM
brj-24573	115	64	bioresources	bioresource	NOUN
brj-24573	115	65	/	/	SYM
brj-24573	115	66	farouk%20et%20al	farouk%20et%20al	PROPN
brj-24573	115	67	..	..	PUNCT
brj-24573	115	68	doc%23_enref_10	doc%23_enref_10	NUM
brj-24573	115	69	peer	peer	NOUN
brj-24573	115	70	-	-	PUNCT
brj-24573	115	71	reviewed	review	VERB
brj-24573	115	72	article	article	NOUN
brj-24573	115	73	bioresources.cnr.ncsu.edu	bioresources.cnr.ncsu.edu	X
brj-24573	115	74	farouk	farouk	PROPN
brj-24573	115	75	et	et	PROPN
brj-24573	115	76	al	al	PROPN
brj-24573	115	77	.	.	PROPN
brj-24573	116	1	(	(	PUNCT
brj-24573	116	2	2025	2025	NUM
brj-24573	116	3	)	)	PUNCT
brj-24573	116	4	.	.	PUNCT
brj-24573	117	1	“	"	PUNCT
brj-24573	117	2	amylase	amylase	NOUN
brj-24573	117	3	via	via	ADP
brj-24573	117	4	xylose	xylose	PROPN
brj-24573	117	5	promoter	promoter	NOUN
brj-24573	117	6	,	,	PUNCT
brj-24573	117	7	”	"	PUNCT
brj-24573	117	8	bioresources	bioresource	NOUN
brj-24573	117	9	20(3	20(3	NOUN
brj-24573	117	10	)	)	PUNCT
brj-24573	117	11	,	,	PUNCT
brj-24573	117	12	7728	7728	NUM
brj-24573	117	13	-	-	SYM
brj-24573	117	14	7737	7737	NUM
brj-24573	117	15	.	.	PUNCT
brj-24573	118	1	7734	7734	NUM
brj-24573	118	2	fig	fig	NOUN
brj-24573	118	3	.	.	PUNCT
brj-24573	119	1	5	5	X
brj-24573	119	2	.	.	X
brj-24573	119	3	activity	activity	NOUN
brj-24573	119	4	of	of	ADP
brj-24573	119	5	recombinant	recombinant	ADJ
brj-24573	119	6	amytv	amytv	NOUN
brj-24573	119	7	in	in	ADP
brj-24573	119	8	b.	b.	PROPN
brj-24573	119	9	subtilis	subtilis	PROPN
brj-24573	119	10	.	.	PUNCT
brj-24573	120	1	bars	bar	NOUN
brj-24573	120	2	show	show	VERB
brj-24573	120	3	α	α	NUM
brj-24573	120	4	-	-	PUNCT
brj-24573	120	5	amylase	amylase	NOUN
brj-24573	120	6	activity	activity	NOUN
brj-24573	120	7	in	in	ADP
brj-24573	120	8	three	three	NUM
brj-24573	120	9	different	different	ADJ
brj-24573	120	10	fractions	fraction	NOUN
brj-24573	120	11	:	:	PUNCT
brj-24573	120	12	the	the	DET
brj-24573	120	13	supernatant	supernatant	NOUN
brj-24573	120	14	(	(	PUNCT
brj-24573	120	15	sn	sn	PROPN
brj-24573	120	16	)	)	PUNCT
brj-24573	120	17	,	,	PUNCT
brj-24573	120	18	the	the	DET
brj-24573	120	19	cell	cell	NOUN
brj-24573	120	20	wall	wall	NOUN
brj-24573	120	21	(	(	PUNCT
brj-24573	120	22	cw	cw	NOUN
brj-24573	120	23	)	)	PUNCT
brj-24573	120	24	,	,	PUNCT
brj-24573	120	25	and	and	CCONJ
brj-24573	120	26	the	the	DET
brj-24573	120	27	cytosol	cytosol	NOUN
brj-24573	120	28	(	(	PUNCT
brj-24573	120	29	cyt	cyt	PROPN
brj-24573	120	30	)	)	PUNCT
brj-24573	120	31	.	.	PUNCT
brj-24573	121	1	“	"	PUNCT
brj-24573	121	2	u	u	NOUN
brj-24573	121	3	”	"	PUNCT
brj-24573	121	4	and	and	CCONJ
brj-24573	121	5	“	"	PUNCT
brj-24573	121	6	i	i	PRON
brj-24573	121	7	”	"	PUNCT
brj-24573	121	8	refer	refer	VERB
brj-24573	121	9	to	to	ADP
brj-24573	121	10	uninduced	uninduced	ADJ
brj-24573	121	11	and	and	CCONJ
brj-24573	121	12	induced	induce	VERB
brj-24573	121	13	,	,	PUNCT
brj-24573	121	14	respectively	respectively	ADV
brj-24573	121	15	.	.	PUNCT
brj-24573	122	1	the	the	DET
brj-24573	122	2	culture	culture	NOUN
brj-24573	122	3	was	be	AUX
brj-24573	122	4	grown	grow	VERB
brj-24573	122	5	in	in	ADP
brj-24573	122	6	2x	2x	NUM
brj-24573	122	7	tby	tby	NOUN
brj-24573	122	8	in	in	ADP
brj-24573	122	9	the	the	DET
brj-24573	122	10	presence	presence	NOUN
brj-24573	122	11	of	of	ADP
brj-24573	122	12	2	2	NUM
brj-24573	122	13	%	%	NOUN
brj-24573	122	14	xylose	xylose	PROPN
brj-24573	122	15	as	as	ADP
brj-24573	122	16	an	an	DET
brj-24573	122	17	inducer	inducer	NOUN
brj-24573	122	18	.	.	PUNCT
brj-24573	123	1	secretion	secretion	NOUN
brj-24573	123	2	of	of	ADP
brj-24573	123	3	the	the	DET
brj-24573	123	4	induced	induced	ADJ
brj-24573	123	5	amytv	amytv	NOUN
brj-24573	123	6	protein	protein	NOUN
brj-24573	123	7	analysis	analysis	NOUN
brj-24573	123	8	of	of	ADP
brj-24573	123	9	secretion	secretion	NOUN
brj-24573	123	10	of	of	ADP
brj-24573	123	11	amytv	amytv	NOUN
brj-24573	123	12	in	in	ADP
brj-24573	123	13	extracellular	extracellular	ADJ
brj-24573	123	14	fraction	fraction	NOUN
brj-24573	123	15	showed	show	VERB
brj-24573	123	16	that	that	SCONJ
brj-24573	123	17	amytv	amytv	PROPN
brj-24573	123	18	was	be	AUX
brj-24573	123	19	completely	completely	ADV
brj-24573	123	20	secreted	secrete	VERB
brj-24573	123	21	under	under	ADP
brj-24573	123	22	the	the	DET
brj-24573	123	23	conditions	condition	NOUN
brj-24573	123	24	of	of	ADP
brj-24573	123	25	inducible	inducible	ADJ
brj-24573	123	26	synthesis	synthesis	NOUN
brj-24573	123	27	.	.	PUNCT
brj-24573	124	1	the	the	DET
brj-24573	124	2	amytv	amytv	NOUN
brj-24573	124	3	residual	residual	ADJ
brj-24573	124	4	activities	activity	NOUN
brj-24573	124	5	in	in	ADP
brj-24573	124	6	the	the	DET
brj-24573	124	7	pellet	pellet	NOUN
brj-24573	124	8	and	and	CCONJ
brj-24573	124	9	cytosol	cytosol	NOUN
brj-24573	124	10	fractions	fraction	NOUN
brj-24573	124	11	were	be	AUX
brj-24573	124	12	0.1	0.1	NUM
brj-24573	124	13	%	%	NOUN
brj-24573	124	14	to	to	PART
brj-24573	124	15	1	1	NUM
brj-24573	124	16	%	%	NOUN
brj-24573	124	17	relative	relative	ADJ
brj-24573	124	18	to	to	ADP
brj-24573	124	19	activity	activity	NOUN
brj-24573	124	20	in	in	ADP
brj-24573	124	21	the	the	DET
brj-24573	124	22	supernatant	supernatant	NOUN
brj-24573	124	23	.	.	PUNCT
brj-24573	125	1	the	the	DET
brj-24573	125	2	assay	assay	NOUN
brj-24573	125	3	also	also	ADV
brj-24573	125	4	demonstrated	demonstrate	VERB
brj-24573	125	5	amytv	amytv	NOUN
brj-24573	125	6	secretion	secretion	NOUN
brj-24573	125	7	30	30	NUM
brj-24573	125	8	min	min	NOUN
brj-24573	125	9	after	after	ADP
brj-24573	125	10	the	the	DET
brj-24573	125	11	induction	induction	NOUN
brj-24573	125	12	,	,	PUNCT
brj-24573	125	13	which	which	PRON
brj-24573	125	14	was	be	AUX
brj-24573	125	15	followed	follow	VERB
brj-24573	125	16	by	by	ADP
brj-24573	125	17	a	a	DET
brj-24573	125	18	sustained	sustained	ADJ
brj-24573	125	19	rise	rise	NOUN
brj-24573	125	20	in	in	ADP
brj-24573	125	21	amylase	amylase	NOUN
brj-24573	125	22	activity	activity	NOUN
brj-24573	125	23	(	(	PUNCT
brj-24573	125	24	fig	fig	NOUN
brj-24573	125	25	.	.	PUNCT
brj-24573	126	1	5	5	NUM
brj-24573	126	2	)	)	PUNCT
brj-24573	126	3	.	.	PUNCT
brj-24573	127	1	discussion	discussion	NOUN
brj-24573	127	2	an	an	DET
brj-24573	127	3	analysis	analysis	NOUN
brj-24573	127	4	of	of	ADP
brj-24573	127	5	the	the	DET
brj-24573	127	6	regulatory	regulatory	ADJ
brj-24573	127	7	promoter	promoter	NOUN
brj-24573	127	8	/	/	SYM
brj-24573	127	9	repressor	repressor	NOUN
brj-24573	127	10	elements	element	NOUN
brj-24573	127	11	from	from	ADP
brj-24573	127	12	the	the	DET
brj-24573	127	13	xylose	xylose	PROPN
brj-24573	127	14	utilizing	utilize	VERB
brj-24573	127	15	operon	operon	NOUN
brj-24573	127	16	of	of	ADP
brj-24573	127	17	b.	b.	PROPN
brj-24573	127	18	megaterium	megaterium	NOUN
brj-24573	127	19	was	be	AUX
brj-24573	127	20	performed	perform	VERB
brj-24573	127	21	in	in	ADP
brj-24573	127	22	b.	b.	PROPN
brj-24573	127	23	subtilis	subtilis	PROPN
brj-24573	127	24	(	(	PUNCT
brj-24573	127	25	gsb26	gsb26	NOUN
brj-24573	127	26	)	)	PUNCT
brj-24573	127	27	using	use	VERB
brj-24573	127	28	amytv	amytv	NOUN
brj-24573	127	29	amylase	amylase	NOUN
brj-24573	127	30	from	from	ADP
brj-24573	127	31	t.	t.	NOUN
brj-24573	127	32	vulgaris	vulgaris	NOUN
brj-24573	127	33	as	as	ADP
brj-24573	127	34	heterologous	heterologous	ADJ
brj-24573	127	35	protein	protein	NOUN
brj-24573	127	36	.	.	PUNCT
brj-24573	128	1	the	the	DET
brj-24573	128	2	pxyla	pxyla	NOUN
brj-24573	128	3	promoter	promoter	NOUN
brj-24573	128	4	was	be	AUX
brj-24573	128	5	found	find	VERB
brj-24573	128	6	to	to	PART
brj-24573	128	7	be	be	AUX
brj-24573	128	8	an	an	DET
brj-24573	128	9	efficient	efficient	ADJ
brj-24573	128	10	system	system	NOUN
brj-24573	128	11	for	for	ADP
brj-24573	128	12	inducible	inducible	ADJ
brj-24573	128	13	secretion	secretion	NOUN
brj-24573	128	14	of	of	ADP
brj-24573	128	15	amytv	amytv	PROPN
brj-24573	128	16	in	in	ADP
brj-24573	128	17	b.	b.	PROPN
brj-24573	128	18	subtilis	subtilis	PROPN
brj-24573	128	19	.	.	PUNCT
brj-24573	129	1	maximum	maximum	ADJ
brj-24573	129	2	expression	expression	NOUN
brj-24573	129	3	was	be	AUX
brj-24573	129	4	observed	observe	VERB
brj-24573	129	5	at	at	ADP
brj-24573	129	6	inducer	inducer	NOUN
brj-24573	129	7	concentrations	concentration	NOUN
brj-24573	129	8	between	between	ADP
brj-24573	129	9	1	1	NUM
brj-24573	129	10	%	%	NOUN
brj-24573	129	11	and	and	CCONJ
brj-24573	129	12	2	2	NUM
brj-24573	129	13	%	%	NOUN
brj-24573	129	14	(	(	PUNCT
brj-24573	129	15	fig	fig	NOUN
brj-24573	129	16	.	.	PUNCT
brj-24573	130	1	3	3	NUM
brj-24573	130	2	)	)	PUNCT
brj-24573	130	3	.	.	PUNCT
brj-24573	131	1	addition	addition	NOUN
brj-24573	131	2	of	of	ADP
brj-24573	131	3	the	the	DET
brj-24573	131	4	inducer	inducer	NOUN
brj-24573	131	5	to	to	ADP
brj-24573	131	6	growing	grow	VERB
brj-24573	131	7	cultures	culture	NOUN
brj-24573	131	8	at	at	ADP
brj-24573	131	9	od540	od540	PROPN
brj-24573	131	10	0.5	0.5	NUM
brj-24573	131	11	resulted	result	VERB
brj-24573	131	12	in	in	ADP
brj-24573	131	13	maximal	maximal	ADJ
brj-24573	131	14	α	α	NOUN
brj-24573	131	15	-	-	PUNCT
brj-24573	131	16	amylase	amylase	NOUN
brj-24573	131	17	activity	activity	NOUN
brj-24573	131	18	.	.	PUNCT
brj-24573	132	1	after	after	ADP
brj-24573	132	2	a	a	DET
brj-24573	132	3	period	period	NOUN
brj-24573	132	4	of	of	ADP
brj-24573	132	5	30	30	NUM
brj-24573	132	6	to	to	PART
brj-24573	132	7	60	60	NUM
brj-24573	132	8	mins	min	NOUN
brj-24573	132	9	,	,	PUNCT
brj-24573	132	10	amytv	amytv	PROPN
brj-24573	132	11	was	be	AUX
brj-24573	132	12	observed	observe	VERB
brj-24573	132	13	to	to	PART
brj-24573	132	14	accumulate	accumulate	VERB
brj-24573	132	15	in	in	ADP
brj-24573	132	16	the	the	DET
brj-24573	132	17	culture	culture	NOUN
brj-24573	132	18	medium	medium	NOUN
brj-24573	132	19	,	,	PUNCT
brj-24573	132	20	with	with	ADP
brj-24573	132	21	the	the	DET
brj-24573	132	22	maximum	maximum	ADJ
brj-24573	132	23	accumulation	accumulation	NOUN
brj-24573	132	24	achieved	achieve	VERB
brj-24573	132	25	after	after	ADP
brj-24573	132	26	approximately	approximately	ADV
brj-24573	132	27	16	16	NUM
brj-24573	132	28	h	h	NOUN
brj-24573	132	29	of	of	ADP
brj-24573	132	30	growth	growth	NOUN
brj-24573	132	31	.	.	PUNCT
brj-24573	133	1	upon	upon	SCONJ
brj-24573	133	2	comparing	compare	VERB
brj-24573	133	3	the	the	DET
brj-24573	133	4	data	datum	NOUN
brj-24573	133	5	from	from	ADP
brj-24573	133	6	the	the	DET
brj-24573	133	7	induction	induction	NOUN
brj-24573	133	8	conditions	condition	NOUN
brj-24573	133	9	in	in	ADP
brj-24573	133	10	bacillus	bacillus	NOUN
brj-24573	133	11	subtilis	subtili	NOUN
brj-24573	133	12	with	with	ADP
brj-24573	133	13	those	those	PRON
brj-24573	133	14	in	in	ADP
brj-24573	133	15	bacillus	bacillus	NOUN
brj-24573	133	16	megaterium	megaterium	NOUN
brj-24573	133	17	(	(	PUNCT
brj-24573	133	18	rygus	rygus	ADJ
brj-24573	133	19	and	and	CCONJ
brj-24573	133	20	hillen	hillen	ADJ
brj-24573	133	21	1991b	1991b	NUM
brj-24573	133	22	)	)	PUNCT
brj-24573	133	23	,	,	PUNCT
brj-24573	133	24	few	few	ADJ
brj-24573	133	25	differences	difference	NOUN
brj-24573	133	26	were	be	AUX
brj-24573	133	27	found	find	VERB
brj-24573	133	28	.	.	PUNCT
brj-24573	134	1	the	the	DET
brj-24573	134	2	optimal	optimal	ADJ
brj-24573	134	3	induction	induction	NOUN
brj-24573	134	4	conditions	condition	NOUN
brj-24573	134	5	for	for	ADP
brj-24573	134	6	bacillus	bacillus	NOUN
brj-24573	134	7	megaterium	megaterium	NOUN
brj-24573	134	8	required	require	VERB
brj-24573	134	9	0.5	0.5	NUM
brj-24573	134	10	%	%	NOUN
brj-24573	134	11	xylose	xylose	PROPN
brj-24573	134	12	,	,	PUNCT
brj-24573	134	13	while	while	SCONJ
brj-24573	134	14	bacillus	bacillus	NOUN
brj-24573	134	15	subtilis	subtili	NOUN
brj-24573	134	16	needed	need	VERB
brj-24573	134	17	at	at	ADP
brj-24573	134	18	least	least	ADV
brj-24573	134	19	twice	twice	DET
brj-24573	134	20	that	that	DET
brj-24573	134	21	amount	amount	NOUN
brj-24573	134	22	of	of	ADP
brj-24573	134	23	inducer	inducer	NOUN
brj-24573	134	24	to	to	PART
brj-24573	134	25	achieve	achieve	VERB
brj-24573	134	26	optimal	optimal	ADJ
brj-24573	134	27	results	result	NOUN
brj-24573	134	28	.	.	PUNCT
brj-24573	135	1	induction	induction	NOUN
brj-24573	135	2	kinetics	kinetic	NOUN
brj-24573	135	3	of	of	ADP
brj-24573	135	4	β	β	NOUN
brj-24573	135	5	-	-	ADJ
brj-24573	135	6	galactosidase	galactosidase	ADJ
brj-24573	135	7	activity	activity	NOUN
brj-24573	135	8	in	in	ADP
brj-24573	135	9	b.	b.	PROPN
brj-24573	135	10	megaterium	megaterium	PROPN
brj-24573	135	11	have	have	AUX
brj-24573	135	12	shown	show	VERB
brj-24573	135	13	that	that	DET
brj-24573	135	14	maximum	maximum	ADJ
brj-24573	135	15	βgalactosidase	βgalactosidase	NOUN
brj-24573	135	16	activity	activity	NOUN
brj-24573	136	1	[	[	X
brj-24573	136	2	u	u	X
brj-24573	136	3	]	]	X
brj-24573	136	4	was	be	AUX
brj-24573	136	5	reached	reach	VERB
brj-24573	136	6	in	in	ADP
brj-24573	136	7	180	180	NUM
brj-24573	136	8	mins	min	NOUN
brj-24573	136	9	(	(	PUNCT
brj-24573	136	10	rygus	rygus	ADJ
brj-24573	136	11	and	and	CCONJ
brj-24573	136	12	hillen	hillen	ADJ
brj-24573	136	13	1991b	1991b	NUM
brj-24573	136	14	)	)	PUNCT
brj-24573	136	15	,	,	PUNCT
brj-24573	136	16	while	while	SCONJ
brj-24573	136	17	the	the	DET
brj-24573	136	18	full	full	ADJ
brj-24573	136	19	level	level	NOUN
brj-24573	136	20	of	of	ADP
brj-24573	136	21	secretion	secretion	NOUN
brj-24573	136	22	in	in	ADP
brj-24573	136	23	b.	b.	PROPN
brj-24573	136	24	subtilis	subtilis	PROPN
brj-24573	136	25	was	be	AUX
brj-24573	136	26	achieved	achieve	VERB
brj-24573	136	27	in	in	ADP
brj-24573	136	28	12	12	NUM
brj-24573	136	29	to	to	PART
brj-24573	136	30	16	16	NUM
brj-24573	136	31	h	h	NOUN
brj-24573	136	32	after	after	ADP
brj-24573	136	33	induction	induction	NOUN
brj-24573	136	34	.	.	PUNCT
brj-24573	137	1	between	between	ADP
brj-24573	137	2	30	30	NUM
brj-24573	137	3	and	and	CCONJ
brj-24573	137	4	60	60	NUM
brj-24573	137	5	min	min	NOUN
brj-24573	137	6	following	follow	VERB
brj-24573	137	7	induction	induction	NOUN
brj-24573	137	8	,	,	PUNCT
brj-24573	137	9	it	it	PRON
brj-24573	137	10	was	be	AUX
brj-24573	137	11	observed	observe	VERB
brj-24573	137	12	that	that	SCONJ
brj-24573	137	13	amytv	amytv	PROPN
brj-24573	137	14	was	be	AUX
brj-24573	137	15	not	not	PART
brj-24573	137	16	found	find	VERB
brj-24573	137	17	to	to	PART
brj-24573	137	18	be	be	AUX
brj-24573	137	19	accumulated	accumulate	VERB
brj-24573	137	20	in	in	ADP
brj-24573	137	21	either	either	CCONJ
brj-24573	137	22	the	the	DET
brj-24573	137	23	cytosolic	cytosolic	ADJ
brj-24573	137	24	or	or	CCONJ
brj-24573	137	25	membrane	membrane	NOUN
brj-24573	137	26	fractions	fraction	NOUN
brj-24573	137	27	of	of	ADP
brj-24573	137	28	the	the	DET
brj-24573	137	29	cells	cell	NOUN
brj-24573	137	30	.	.	PUNCT
brj-24573	138	1	instead	instead	ADV
brj-24573	138	2	,	,	PUNCT
brj-24573	138	3	it	it	PRON
brj-24573	138	4	was	be	AUX
brj-24573	138	5	file:///d:/post%20phd	file:///d:/post%20phd	NOUN
brj-24573	138	6	/	/	SYM
brj-24573	138	7	dr.%20farouk%20taif	dr.%20farouk%20taif	PROPN
brj-24573	138	8	/	/	SYM
brj-24573	138	9	manucript%201	manucript%201	PROPN
brj-24573	138	10	/	/	SYM
brj-24573	138	11	amylase	amylase	NOUN
brj-24573	138	12	/	/	SYM
brj-24573	138	13	bioresources	bioresource	NOUN
brj-24573	138	14	/	/	SYM
brj-24573	138	15	farouk%20et%20al	farouk%20et%20al	PROPN
brj-24573	138	16	..	..	PUNCT
brj-24573	138	17	doc%23_enref_16	doc%23_enref_16	NOUN
brj-24573	138	18	peer	peer	NOUN
brj-24573	138	19	-	-	PUNCT
brj-24573	138	20	reviewed	review	VERB
brj-24573	138	21	article	article	NOUN
brj-24573	138	22	bioresources.cnr.ncsu.edu	bioresources.cnr.ncsu.edu	X
brj-24573	138	23	farouk	farouk	PROPN
brj-24573	138	24	et	et	PROPN
brj-24573	138	25	al	al	PROPN
brj-24573	138	26	.	.	PROPN
brj-24573	139	1	(	(	PUNCT
brj-24573	139	2	2025	2025	NUM
brj-24573	139	3	)	)	PUNCT
brj-24573	139	4	.	.	PUNCT
brj-24573	140	1	“	"	PUNCT
brj-24573	140	2	amylase	amylase	NOUN
brj-24573	140	3	via	via	ADP
brj-24573	140	4	xylose	xylose	PROPN
brj-24573	140	5	promoter	promoter	NOUN
brj-24573	140	6	,	,	PUNCT
brj-24573	140	7	”	"	PUNCT
brj-24573	140	8	bioresources	bioresource	NOUN
brj-24573	140	9	20(3	20(3	NOUN
brj-24573	140	10	)	)	PUNCT
brj-24573	140	11	,	,	PUNCT
brj-24573	140	12	7728	7728	NUM
brj-24573	140	13	-	-	SYM
brj-24573	140	14	7737	7737	NUM
brj-24573	140	15	.	.	PUNCT
brj-24573	141	1	7735	7735	NUM
brj-24573	141	2	completely	completely	ADV
brj-24573	141	3	secreted	secrete	VERB
brj-24573	141	4	into	into	ADP
brj-24573	141	5	the	the	DET
brj-24573	141	6	growth	growth	NOUN
brj-24573	141	7	medium	medium	NOUN
brj-24573	141	8	.	.	PUNCT
brj-24573	142	1	the	the	DET
brj-24573	142	2	findings	finding	NOUN
brj-24573	142	3	suggest	suggest	VERB
brj-24573	142	4	that	that	SCONJ
brj-24573	142	5	the	the	DET
brj-24573	142	6	secretory	secretory	ADJ
brj-24573	142	7	pathway	pathway	NOUN
brj-24573	142	8	of	of	ADP
brj-24573	142	9	b.	b.	PROPN
brj-24573	142	10	subtilis	subtilis	PROPN
brj-24573	142	11	demonstrated	demonstrate	VERB
brj-24573	142	12	remarkable	remarkable	ADJ
brj-24573	142	13	potential	potential	NOUN
brj-24573	142	14	in	in	ADP
brj-24573	142	15	efficiently	efficiently	ADV
brj-24573	142	16	processing	process	VERB
brj-24573	142	17	and	and	CCONJ
brj-24573	142	18	releasing	release	VERB
brj-24573	142	19	fully	fully	ADV
brj-24573	142	20	matured	mature	VERB
brj-24573	142	21	amylase	amylase	NOUN
brj-24573	142	22	,	,	PUNCT
brj-24573	142	23	even	even	ADV
brj-24573	142	24	after	after	ADP
brj-24573	142	25	a	a	DET
brj-24573	142	26	significant	significant	ADJ
brj-24573	142	27	increase	increase	NOUN
brj-24573	142	28	in	in	ADP
brj-24573	142	29	amytv	amytv	NOUN
brj-24573	142	30	synthesis	synthesis	NOUN
brj-24573	142	31	following	follow	VERB
brj-24573	142	32	xylose	xylose	PROPN
brj-24573	142	33	induction	induction	NOUN
brj-24573	142	34	.	.	PUNCT
brj-24573	143	1	the	the	DET
brj-24573	143	2	xylose	xylose	PROPN
brj-24573	143	3	inducible	inducible	ADJ
brj-24573	143	4	system	system	NOUN
brj-24573	143	5	could	could	AUX
brj-24573	143	6	therefore	therefore	ADV
brj-24573	143	7	efficiently	efficiently	ADV
brj-24573	143	8	be	be	AUX
brj-24573	143	9	used	use	VERB
brj-24573	143	10	for	for	ADP
brj-24573	143	11	producing	produce	VERB
brj-24573	143	12	secretory	secretory	ADJ
brj-24573	143	13	proteins	protein	NOUN
brj-24573	143	14	in	in	ADP
brj-24573	143	15	b.	b.	PROPN
brj-24573	143	16	subtilis	subtilis	PROPN
brj-24573	143	17	.	.	PUNCT
brj-24573	144	1	while	while	SCONJ
brj-24573	144	2	xylose	xylose	PROPN
brj-24573	144	3	enhanced	enhance	VERB
brj-24573	144	4	enzyme	enzyme	NOUN
brj-24573	144	5	expression	expression	NOUN
brj-24573	144	6	under	under	ADP
brj-24573	144	7	the	the	DET
brj-24573	144	8	control	control	NOUN
brj-24573	144	9	of	of	ADP
brj-24573	144	10	the	the	DET
brj-24573	144	11	xylose	xylose	PROPN
brj-24573	144	12	-	-	PUNCT
brj-24573	144	13	inducible	inducible	ADJ
brj-24573	144	14	promoter	promoter	NOUN
brj-24573	144	15	,	,	PUNCT
brj-24573	144	16	further	further	ADJ
brj-24573	144	17	experiments	experiment	NOUN
brj-24573	144	18	using	use	VERB
brj-24573	144	19	non	non	ADJ
brj-24573	144	20	-	-	ADJ
brj-24573	144	21	metabolizable	metabolizable	ADJ
brj-24573	144	22	analogs	analog	NOUN
brj-24573	144	23	would	would	AUX
brj-24573	144	24	be	be	AUX
brj-24573	144	25	required	require	VERB
brj-24573	144	26	to	to	PART
brj-24573	144	27	confirm	confirm	VERB
brj-24573	144	28	its	its	PRON
brj-24573	144	29	role	role	NOUN
brj-24573	144	30	as	as	ADP
brj-24573	144	31	a	a	DET
brj-24573	144	32	true	true	ADJ
brj-24573	144	33	inducer	inducer	NOUN
brj-24573	144	34	independent	independent	ADJ
brj-24573	144	35	of	of	ADP
brj-24573	144	36	its	its	PRON
brj-24573	144	37	catabolic	catabolic	ADJ
brj-24573	144	38	fate	fate	NOUN
brj-24573	144	39	.	.	PUNCT
brj-24573	145	1	furthermore	furthermore	ADV
brj-24573	145	2	,	,	PUNCT
brj-24573	145	3	to	to	PART
brj-24573	145	4	act	act	VERB
brj-24573	145	5	as	as	ADP
brj-24573	145	6	an	an	DET
brj-24573	145	7	inducer	inducer	NOUN
brj-24573	145	8	,	,	PUNCT
brj-24573	145	9	the	the	DET
brj-24573	145	10	sugar	sugar	NOUN
brj-24573	145	11	must	must	AUX
brj-24573	145	12	have	have	VERB
brj-24573	145	13	a	a	DET
brj-24573	145	14	binding	bind	VERB
brj-24573	145	15	site	site	NOUN
brj-24573	145	16	for	for	ADP
brj-24573	145	17	attaching	attach	VERB
brj-24573	145	18	to	to	ADP
brj-24573	145	19	the	the	DET
brj-24573	145	20	repressor	repressor	NOUN
brj-24573	145	21	protein	protein	NOUN
brj-24573	145	22	.	.	PUNCT
brj-24573	146	1	this	this	DET
brj-24573	146	2	binding	bind	VERB
brj-24573	146	3	dissociates	dissociate	NOUN
brj-24573	146	4	repressor	repressor	NOUN
brj-24573	146	5	from	from	ADP
brj-24573	146	6	the	the	DET
brj-24573	146	7	promoter	promoter	NOUN
brj-24573	146	8	and	and	CCONJ
brj-24573	146	9	hence	hence	ADV
brj-24573	146	10	starts	start	VERB
brj-24573	146	11	transcription	transcription	NOUN
brj-24573	146	12	.	.	PUNCT
brj-24573	147	1	repressor	repressor	NOUN
brj-24573	147	2	proteins	protein	NOUN
brj-24573	147	3	do	do	AUX
brj-24573	147	4	not	not	PART
brj-24573	147	5	have	have	VERB
brj-24573	147	6	binding	bind	VERB
brj-24573	147	7	sites	site	NOUN
brj-24573	147	8	for	for	ADP
brj-24573	147	9	all	all	DET
brj-24573	147	10	sugars	sugar	NOUN
brj-24573	147	11	,	,	PUNCT
brj-24573	147	12	and	and	CCONJ
brj-24573	147	13	hence	hence	ADV
brj-24573	147	14	not	not	PART
brj-24573	147	15	all	all	DET
brj-24573	147	16	sugars	sugar	NOUN
brj-24573	147	17	can	can	AUX
brj-24573	147	18	act	act	VERB
brj-24573	147	19	as	as	ADP
brj-24573	147	20	inducers	inducer	NOUN
brj-24573	147	21	.	.	PUNCT
brj-24573	148	1	therefore	therefore	ADV
brj-24573	148	2	,	,	PUNCT
brj-24573	148	3	this	this	DET
brj-24573	148	4	study	study	NOUN
brj-24573	148	5	successfully	successfully	ADV
brj-24573	148	6	confirmed	confirm	VERB
brj-24573	148	7	the	the	DET
brj-24573	148	8	hypothesis	hypothesis	NOUN
brj-24573	148	9	that	that	PRON
brj-24573	148	10	xylose	xylose	PROPN
brj-24573	148	11	acts	act	VERB
brj-24573	148	12	as	as	ADP
brj-24573	148	13	a	a	DET
brj-24573	148	14	potential	potential	ADJ
brj-24573	148	15	inducer	inducer	NOUN
brj-24573	148	16	.	.	PUNCT
brj-24573	149	1	the	the	DET
brj-24573	149	2	xylose	xylose	PROPN
brj-24573	149	3	inducible	inducible	ADJ
brj-24573	149	4	systems	system	NOUN
brj-24573	149	5	can	can	AUX
brj-24573	149	6	avoid	avoid	VERB
brj-24573	149	7	proteolytic	proteolytic	ADJ
brj-24573	149	8	degradation	degradation	NOUN
brj-24573	149	9	of	of	ADP
brj-24573	149	10	heterologous	heterologous	ADJ
brj-24573	149	11	proteins	protein	NOUN
brj-24573	149	12	in	in	ADP
brj-24573	149	13	b.	b.	PROPN
brj-24573	149	14	subtilis	subtili	NOUN
brj-24573	149	15	by	by	ADP
brj-24573	149	16	selecting	select	VERB
brj-24573	149	17	the	the	DET
brj-24573	149	18	phase	phase	NOUN
brj-24573	149	19	of	of	ADP
brj-24573	149	20	expression	expression	NOUN
brj-24573	149	21	as	as	SCONJ
brj-24573	149	22	controlled	control	VERB
brj-24573	149	23	by	by	ADP
brj-24573	149	24	addition	addition	NOUN
brj-24573	149	25	of	of	ADP
brj-24573	149	26	the	the	DET
brj-24573	149	27	inducer	inducer	NOUN
brj-24573	149	28	.	.	PUNCT
brj-24573	150	1	when	when	SCONJ
brj-24573	150	2	the	the	DET
brj-24573	150	3	regulation	regulation	NOUN
brj-24573	150	4	and	and	CCONJ
brj-24573	150	5	strength	strength	NOUN
brj-24573	150	6	of	of	ADP
brj-24573	150	7	the	the	DET
brj-24573	150	8	pxyla	pxyla	NOUN
brj-24573	150	9	promoter	promoter	NOUN
brj-24573	150	10	in	in	ADP
brj-24573	150	11	b.	b.	PROPN
brj-24573	150	12	megaterium	megaterium	NOUN
brj-24573	150	13	was	be	AUX
brj-24573	150	14	studied	study	VERB
brj-24573	150	15	,	,	PUNCT
brj-24573	150	16	the	the	DET
brj-24573	150	17	expression	expression	NOUN
brj-24573	150	18	was	be	AUX
brj-24573	150	19	found	find	VERB
brj-24573	150	20	150	150	NUM
brj-24573	150	21	-	-	ADJ
brj-24573	150	22	fold	fold	NOUN
brj-24573	150	23	inducible	inducible	ADJ
brj-24573	150	24	by	by	ADP
brj-24573	150	25	xylose	xylose	PROPN
brj-24573	150	26	(	(	PUNCT
brj-24573	150	27	rygus	rygus	NOUN
brj-24573	150	28	and	and	CCONJ
brj-24573	150	29	hillen	hillen	ADJ
brj-24573	150	30	1992	1992	NUM
brj-24573	150	31	)	)	PUNCT
brj-24573	150	32	.	.	PUNCT
brj-24573	151	1	in	in	ADP
brj-24573	151	2	this	this	DET
brj-24573	151	3	study	study	NOUN
brj-24573	151	4	,	,	PUNCT
brj-24573	151	5	comparable	comparable	ADJ
brj-24573	151	6	results	result	NOUN
brj-24573	151	7	were	be	AUX
brj-24573	151	8	obtained	obtain	VERB
brj-24573	151	9	for	for	ADP
brj-24573	151	10	secretory	secretory	ADJ
brj-24573	151	11	expression	expression	NOUN
brj-24573	151	12	in	in	ADP
brj-24573	151	13	b.	b.	PROPN
brj-24573	151	14	subtilis	subtilis	PROPN
brj-24573	151	15	.	.	PUNCT
brj-24573	152	1	the	the	DET
brj-24573	152	2	expression	expression	NOUN
brj-24573	152	3	of	of	ADP
brj-24573	152	4	amytv	amytv	PROPN
brj-24573	152	5	in	in	ADP
brj-24573	152	6	b.	b.	PROPN
brj-24573	152	7	subtilis	subtilis	PROPN
brj-24573	152	8	(	(	PUNCT
brj-24573	152	9	gsb26	gsb26	NOUN
brj-24573	152	10	)	)	PUNCT
brj-24573	152	11	was	be	AUX
brj-24573	152	12	enhanced	enhance	VERB
brj-24573	152	13	500	500	NUM
brj-24573	152	14	-	-	NOUN
brj-24573	152	15	fold	fold	ADJ
brj-24573	152	16	after	after	ADP
brj-24573	152	17	xylose	xylose	PROPN
brj-24573	152	18	induction	induction	NOUN
brj-24573	152	19	.	.	PUNCT
brj-24573	153	1	the	the	DET
brj-24573	153	2	mechanism	mechanism	NOUN
brj-24573	153	3	of	of	ADP
brj-24573	153	4	catabolite	catabolite	ADJ
brj-24573	153	5	repression	repression	NOUN
brj-24573	153	6	in	in	ADP
brj-24573	153	7	b.	b.	PROPN
brj-24573	153	8	subtilis	subtilis	PROPN
brj-24573	153	9	and	and	CCONJ
brj-24573	153	10	gram	gram	NOUN
brj-24573	153	11	-	-	PUNCT
brj-24573	153	12	positive	positive	ADJ
brj-24573	153	13	bacteria	bacteria	NOUN
brj-24573	153	14	is	be	AUX
brj-24573	153	15	complex	complex	ADJ
brj-24573	153	16	and	and	CCONJ
brj-24573	153	17	is	be	AUX
brj-24573	153	18	still	still	ADV
brj-24573	153	19	a	a	DET
brj-24573	153	20	matter	matter	NOUN
brj-24573	153	21	of	of	ADP
brj-24573	153	22	investigation	investigation	NOUN
brj-24573	153	23	(	(	PUNCT
brj-24573	153	24	hueck	hueck	NOUN
brj-24573	153	25	and	and	CCONJ
brj-24573	153	26	hillen	hillen	ADJ
brj-24573	153	27	1995	1995	NUM
brj-24573	153	28	;	;	PUNCT
brj-24573	153	29	liu	liu	PROPN
brj-24573	153	30	et	et	PROPN
brj-24573	153	31	al	al	PROPN
brj-24573	153	32	.	.	PROPN
brj-24573	153	33	2022	2022	NUM
brj-24573	153	34	)	)	PUNCT
brj-24573	153	35	.	.	PUNCT
brj-24573	154	1	in	in	ADP
brj-24573	154	2	this	this	DET
brj-24573	154	3	study	study	NOUN
brj-24573	154	4	,	,	PUNCT
brj-24573	154	5	it	it	PRON
brj-24573	154	6	was	be	AUX
brj-24573	154	7	interesting	interesting	ADJ
brj-24573	154	8	to	to	PART
brj-24573	154	9	observe	observe	VERB
brj-24573	154	10	that	that	SCONJ
brj-24573	154	11	the	the	DET
brj-24573	154	12	activation	activation	NOUN
brj-24573	154	13	of	of	ADP
brj-24573	154	14	the	the	DET
brj-24573	154	15	xylose	xylose	PROPN
brj-24573	154	16	-	-	PUNCT
brj-24573	154	17	inducible	inducible	ADJ
brj-24573	154	18	system	system	NOUN
brj-24573	154	19	could	could	AUX
brj-24573	154	20	be	be	AUX
brj-24573	154	21	promptly	promptly	ADV
brj-24573	154	22	halted	halt	VERB
brj-24573	154	23	at	at	ADP
brj-24573	154	24	any	any	DET
brj-24573	154	25	point	point	NOUN
brj-24573	154	26	during	during	ADP
brj-24573	154	27	the	the	DET
brj-24573	154	28	induction	induction	NOUN
brj-24573	154	29	period	period	NOUN
brj-24573	154	30	by	by	ADP
brj-24573	154	31	simply	simply	ADV
brj-24573	154	32	adding	add	VERB
brj-24573	154	33	glucose	glucose	NOUN
brj-24573	154	34	,	,	PUNCT
brj-24573	154	35	the	the	DET
brj-24573	154	36	catabolite	catabolite	ADJ
brj-24573	154	37	repressor	repressor	NOUN
brj-24573	154	38	(	(	PUNCT
brj-24573	154	39	fig	fig	NOUN
brj-24573	154	40	.	.	PUNCT
brj-24573	154	41	4	4	NUM
brj-24573	154	42	)	)	PUNCT
brj-24573	154	43	.	.	PUNCT
brj-24573	155	1	this	this	DET
brj-24573	155	2	finding	finding	NOUN
brj-24573	155	3	suggested	suggest	VERB
brj-24573	155	4	that	that	SCONJ
brj-24573	155	5	the	the	DET
brj-24573	155	6	potential	potential	ADJ
brj-24573	155	7	target	target	NOUN
brj-24573	155	8	,	,	PUNCT
brj-24573	155	9	likely	likely	ADV
brj-24573	155	10	the	the	DET
brj-24573	155	11	repressor	repressor	NOUN
brj-24573	155	12	xylr	xylr	PROPN
brj-24573	155	13	,	,	PUNCT
brj-24573	155	14	possesses	possess	VERB
brj-24573	155	15	a	a	DET
brj-24573	155	16	balanced	balanced	ADJ
brj-24573	155	17	affinity	affinity	NOUN
brj-24573	155	18	for	for	ADP
brj-24573	155	19	both	both	CCONJ
brj-24573	155	20	xylose	xylose	PROPN
brj-24573	155	21	and	and	CCONJ
brj-24573	155	22	glucose	glucose	NOUN
brj-24573	155	23	.	.	PUNCT
brj-24573	156	1	additionally	additionally	ADV
brj-24573	156	2	,	,	PUNCT
brj-24573	156	3	it	it	PRON
brj-24573	156	4	appeared	appear	VERB
brj-24573	156	5	that	that	SCONJ
brj-24573	156	6	xylr	xylr	PROPN
brj-24573	156	7	could	could	AUX
brj-24573	156	8	be	be	AUX
brj-24573	156	9	inactivated	inactivate	VERB
brj-24573	156	10	and	and	CCONJ
brj-24573	156	11	then	then	ADV
brj-24573	156	12	reactivated	reactivate	VERB
brj-24573	156	13	in	in	ADP
brj-24573	156	14	response	response	NOUN
brj-24573	156	15	to	to	ADP
brj-24573	156	16	consecutive	consecutive	ADJ
brj-24573	156	17	changes	change	NOUN
brj-24573	156	18	in	in	ADP
brj-24573	156	19	the	the	DET
brj-24573	156	20	presence	presence	NOUN
brj-24573	156	21	of	of	ADP
brj-24573	156	22	these	these	DET
brj-24573	156	23	sugars	sugar	NOUN
brj-24573	156	24	.	.	PUNCT
brj-24573	157	1	conclusions	conclusion	NOUN
brj-24573	157	2	1	1	NUM
brj-24573	157	3	.	.	PUNCT
brj-24573	158	1	an	an	DET
brj-24573	158	2	expression	expression	NOUN
brj-24573	158	3	cassette	cassette	NOUN
brj-24573	158	4	constructed	construct	VERB
brj-24573	158	5	using	use	VERB
brj-24573	158	6	b.	b.	PROPN
brj-24573	158	7	megaterium	megaterium	PROPN
brj-24573	158	8	pwh1520	pwh1520	PROPN
brj-24573	158	9	vector	vector	NOUN
brj-24573	158	10	having	have	VERB
brj-24573	158	11	pxyla	pxyla	NOUN
brj-24573	158	12	promoter	promoter	NOUN
brj-24573	158	13	containing	contain	VERB
brj-24573	158	14	a	a	DET
brj-24573	158	15	glucose	glucose	NOUN
brj-24573	158	16	box	box	NOUN
brj-24573	158	17	region	region	NOUN
brj-24573	158	18	,	,	PUNCT
brj-24573	158	19	a	a	DET
brj-24573	158	20	xylose	xylose	PROPN
brj-24573	158	21	repressor	repressor	NOUN
brj-24573	158	22	gene	gene	NOUN
brj-24573	158	23	xylr	xylr	PROPN
brj-24573	158	24	was	be	AUX
brj-24573	158	25	successfully	successfully	ADV
brj-24573	158	26	used	use	VERB
brj-24573	158	27	for	for	ADP
brj-24573	158	28	amylase	amylase	NOUN
brj-24573	158	29	secretion	secretion	NOUN
brj-24573	158	30	studies	study	NOUN
brj-24573	158	31	in	in	ADP
brj-24573	158	32	b.	b.	PROPN
brj-24573	158	33	subtilis	subtilis	PROPN
brj-24573	158	34	(	(	PUNCT
brj-24573	158	35	gsb26	gsb26	NOUN
brj-24573	158	36	)	)	PUNCT
brj-24573	158	37	.	.	PUNCT
brj-24573	159	1	2	2	X
brj-24573	159	2	.	.	X
brj-24573	159	3	when	when	SCONJ
brj-24573	159	4	synthesizing	synthesize	VERB
brj-24573	159	5	the	the	DET
brj-24573	159	6	amytv	amytv	NOUN
brj-24573	159	7	protein	protein	NOUN
brj-24573	159	8	,	,	PUNCT
brj-24573	159	9	it	it	PRON
brj-24573	159	10	was	be	AUX
brj-24573	159	11	found	find	VERB
brj-24573	159	12	that	that	SCONJ
brj-24573	159	13	the	the	DET
brj-24573	159	14	highest	high	ADJ
brj-24573	159	15	amount	amount	NOUN
brj-24573	159	16	of	of	ADP
brj-24573	159	17	amylase	amylase	NOUN
brj-24573	159	18	production	production	NOUN
brj-24573	159	19	was	be	AUX
brj-24573	159	20	achieved	achieve	VERB
brj-24573	159	21	using	use	VERB
brj-24573	159	22	2	2	NUM
brj-24573	159	23	%	%	NOUN
brj-24573	159	24	xylose	xylose	PROPN
brj-24573	159	25	as	as	ADP
brj-24573	159	26	the	the	DET
brj-24573	159	27	inducer	inducer	NOUN
brj-24573	159	28	.	.	PUNCT
brj-24573	160	1	3	3	X
brj-24573	160	2	.	.	X
brj-24573	160	3	experiments	experiment	NOUN
brj-24573	160	4	where	where	SCONJ
brj-24573	160	5	glucose	glucose	NOUN
brj-24573	160	6	or	or	CCONJ
brj-24573	160	7	a	a	DET
brj-24573	160	8	combination	combination	NOUN
brj-24573	160	9	of	of	ADP
brj-24573	160	10	glucose	glucose	NOUN
brj-24573	160	11	and	and	CCONJ
brj-24573	160	12	xylose	xylose	PROPN
brj-24573	160	13	were	be	AUX
brj-24573	160	14	used	use	VERB
brj-24573	160	15	as	as	ADP
brj-24573	160	16	inducers	inducer	NOUN
brj-24573	160	17	demonstrated	demonstrate	VERB
brj-24573	160	18	that	that	SCONJ
brj-24573	160	19	glucose	glucose	NOUN
brj-24573	160	20	functioned	function	VERB
brj-24573	160	21	as	as	ADP
brj-24573	160	22	a	a	DET
brj-24573	160	23	repressor	repressor	NOUN
brj-24573	160	24	of	of	ADP
brj-24573	160	25	amytv	amytv	NOUN
brj-24573	160	26	expression	expression	NOUN
brj-24573	160	27	.	.	PUNCT
brj-24573	161	1	4	4	X
brj-24573	161	2	.	.	X
brj-24573	161	3	the	the	DET
brj-24573	161	4	protein	protein	NOUN
brj-24573	161	5	was	be	AUX
brj-24573	161	6	efficiently	efficiently	ADV
brj-24573	161	7	secreted	secrete	VERB
brj-24573	161	8	and	and	CCONJ
brj-24573	161	9	did	do	AUX
brj-24573	161	10	not	not	PART
brj-24573	161	11	accumulate	accumulate	VERB
brj-24573	161	12	in	in	ADP
brj-24573	161	13	the	the	DET
brj-24573	161	14	cellular	cellular	ADJ
brj-24573	161	15	fractions	fraction	NOUN
brj-24573	161	16	,	,	PUNCT
brj-24573	161	17	even	even	ADV
brj-24573	161	18	at	at	ADP
brj-24573	161	19	high	high	ADJ
brj-24573	161	20	expression	expression	NOUN
brj-24573	161	21	levels	level	NOUN
brj-24573	161	22	.	.	PUNCT
brj-24573	162	1	file:///d:/post%20phd	file:///d:/post%20phd	NOUN
brj-24573	162	2	/	/	SYM
brj-24573	162	3	dr.%20farouk%20taif	dr.%20farouk%20taif	PROPN
brj-24573	162	4	/	/	SYM
brj-24573	162	5	manucript%201	manucript%201	PROPN
brj-24573	162	6	/	/	SYM
brj-24573	162	7	amylase	amylase	NOUN
brj-24573	162	8	/	/	SYM
brj-24573	162	9	bioresources	bioresource	NOUN
brj-24573	162	10	/	/	SYM
brj-24573	162	11	farouk%20et%20al	farouk%20et%20al	PROPN
brj-24573	162	12	..	..	PUNCT
brj-24573	162	13	doc%23_enref_17	doc%23_enref_17	PROPN
brj-24573	162	14	file:///d:/post%20phd	file:///d:/post%20phd	NOUN
brj-24573	162	15	/	/	SYM
brj-24573	162	16	dr.%20farouk%20taif	dr.%20farouk%20taif	PROPN
brj-24573	162	17	/	/	SYM
brj-24573	162	18	manucript%201	manucript%201	PROPN
brj-24573	162	19	/	/	SYM
brj-24573	162	20	amylase	amylase	NOUN
brj-24573	162	21	/	/	SYM
brj-24573	162	22	bioresources	bioresource	NOUN
brj-24573	162	23	/	/	SYM
brj-24573	162	24	farouk%20et%20al	farouk%20et%20al	PROPN
brj-24573	162	25	..	..	PUNCT
brj-24573	162	26	doc%23_enref_6	doc%23_enref_6	ADV
brj-24573	162	27	file:///d:/post%20phd	file:///d:/post%20phd	NOUN
brj-24573	162	28	/	/	SYM
brj-24573	162	29	dr.%20farouk%20taif	dr.%20farouk%20taif	PROPN
brj-24573	162	30	/	/	SYM
brj-24573	162	31	manucript%201	manucript%201	PROPN
brj-24573	162	32	/	/	SYM
brj-24573	162	33	amylase	amylase	NOUN
brj-24573	162	34	/	/	SYM
brj-24573	162	35	bioresources	bioresource	NOUN
brj-24573	162	36	/	/	SYM
brj-24573	162	37	farouk%20et%20al	farouk%20et%20al	PROPN
brj-24573	162	38	..	..	PUNCT
brj-24573	162	39	doc%23_enref_10	doc%23_enref_10	NUM
brj-24573	162	40	peer	peer	NOUN
brj-24573	162	41	-	-	PUNCT
brj-24573	162	42	reviewed	review	VERB
brj-24573	162	43	article	article	NOUN
brj-24573	162	44	bioresources.cnr.ncsu.edu	bioresources.cnr.ncsu.edu	X
brj-24573	162	45	farouk	farouk	PROPN
brj-24573	162	46	et	et	PROPN
brj-24573	162	47	al	al	PROPN
brj-24573	162	48	.	.	PROPN
brj-24573	163	1	(	(	PUNCT
brj-24573	163	2	2025	2025	NUM
brj-24573	163	3	)	)	PUNCT
brj-24573	163	4	.	.	PUNCT
brj-24573	164	1	“	"	PUNCT
brj-24573	164	2	amylase	amylase	NOUN
brj-24573	164	3	via	via	ADP
brj-24573	164	4	xylose	xylose	PROPN
brj-24573	164	5	promoter	promoter	NOUN
brj-24573	164	6	,	,	PUNCT
brj-24573	164	7	”	"	PUNCT
brj-24573	164	8	bioresources	bioresource	NOUN
brj-24573	164	9	20(3	20(3	NOUN
brj-24573	164	10	)	)	PUNCT
brj-24573	164	11	,	,	PUNCT
brj-24573	164	12	7728	7728	NUM
brj-24573	164	13	-	-	SYM
brj-24573	164	14	7737	7737	NUM
brj-24573	164	15	.	.	PUNCT
brj-24573	165	1	7736	7736	NUM
brj-24573	165	2	acknowledgments	acknowledgment	NOUN
brj-24573	165	3	the	the	DET
brj-24573	165	4	authors	author	NOUN
brj-24573	165	5	extend	extend	VERB
brj-24573	165	6	their	their	PRON
brj-24573	165	7	appreciation	appreciation	NOUN
brj-24573	165	8	to	to	ADP
brj-24573	165	9	taif	taif	PROPN
brj-24573	165	10	university	university	PROPN
brj-24573	165	11	,	,	PUNCT
brj-24573	165	12	saudi	saudi	PROPN
brj-24573	165	13	arabia	arabia	PROPN
brj-24573	165	14	,	,	PUNCT
brj-24573	165	15	for	for	ADP
brj-24573	165	16	supporting	support	VERB
brj-24573	165	17	this	this	DET
brj-24573	165	18	work	work	NOUN
brj-24573	165	19	through	through	ADP
brj-24573	165	20	project	project	NOUN
brj-24573	165	21	number	number	NOUN
brj-24573	165	22	(	(	PUNCT
brj-24573	165	23	tu	tu	NOUN
brj-24573	165	24	-	-	PUNCT
brj-24573	165	25	dspp-2024	dspp-2024	NOUN
brj-24573	165	26	-	-	PUNCT
brj-24573	165	27	65	65	NUM
brj-24573	165	28	)	)	PUNCT
brj-24573	165	29	.	.	PUNCT
brj-24573	166	1	funding	fund	VERB
brj-24573	166	2	this	this	DET
brj-24573	166	3	research	research	NOUN
brj-24573	166	4	was	be	AUX
brj-24573	166	5	funded	fund	VERB
brj-24573	166	6	by	by	ADP
brj-24573	166	7	taif	taif	PROPN
brj-24573	166	8	university	university	PROPN
brj-24573	166	9	,	,	PUNCT
brj-24573	166	10	saudi	saudi	PROPN
brj-24573	166	11	arabia	arabia	PROPN
brj-24573	166	12	,	,	PUNCT
brj-24573	166	13	project	project	NOUN
brj-24573	166	14	no	no	INTJ
brj-24573	166	15	.	.	PUNCT
brj-24573	167	1	(	(	PUNCT
brj-24573	167	2	tu	tu	NOUN
brj-24573	167	3	-	-	NOUN
brj-24573	167	4	dspp-202465	dspp-202465	NOUN
brj-24573	167	5	)	)	PUNCT
brj-24573	167	6	.	.	PUNCT
brj-24573	168	1	references	reference	NOUN
brj-24573	168	2	cited	cite	VERB
brj-24573	168	3	aroob	aroob	PROPN
brj-24573	168	4	,	,	PUNCT
brj-24573	168	5	i.	i.	PROPN
brj-24573	168	6	,	,	PUNCT
brj-24573	168	7	ahmad	ahmad	PROPN
brj-24573	168	8	,	,	PUNCT
brj-24573	168	9	n.	n.	NOUN
brj-24573	168	10	,	,	PUNCT
brj-24573	168	11	and	and	CCONJ
brj-24573	168	12	rashid	rashid	PROPN
brj-24573	168	13	,	,	PUNCT
brj-24573	168	14	n.	n.	NOUN
brj-24573	168	15	(	(	PUNCT
brj-24573	168	16	2021	2021	NUM
brj-24573	168	17	)	)	PUNCT
brj-24573	168	18	.	.	PUNCT
brj-24573	169	1	"	"	PUNCT
brj-24573	169	2	cyclodextrin	cyclodextrin	NOUN
brj-24573	169	3	-	-	PUNCT
brj-24573	169	4	preferring	prefer	VERB
brj-24573	169	5	glycoside	glycoside	NOUN
brj-24573	169	6	hydrolases	hydrolase	NOUN
brj-24573	169	7	:	:	PUNCT
brj-24573	169	8	properties	property	NOUN
brj-24573	169	9	and	and	CCONJ
brj-24573	169	10	applications	application	NOUN
brj-24573	169	11	,	,	PUNCT
brj-24573	169	12	"	"	PUNCT
brj-24573	169	13	amylase	amylase	NOUN
brj-24573	169	14	5(1	5(1	NUM
brj-24573	169	15	)	)	PUNCT
brj-24573	169	16	,	,	PUNCT
brj-24573	169	17	23	23	NUM
brj-24573	169	18	-	-	SYM
brj-24573	169	19	37	37	NUM
brj-24573	169	20	.	.	PUNCT
brj-24573	170	1	doi	doi	NOUN
brj-24573	170	2	:	:	PUNCT
brj-24573	170	3	10.1515	10.1515	NUM
brj-24573	170	4	/	/	SYM
brj-24573	170	5	amylase-2021	amylase-2021	ADJ
brj-24573	170	6	-	-	PUNCT
brj-24573	170	7	0003	0003	NUM
brj-24573	170	8	aroob	aroob	PROPN
brj-24573	170	9	,	,	PUNCT
brj-24573	170	10	i.	i.	NOUN
brj-24573	170	11	,	,	PUNCT
brj-24573	170	12	javed	javed	PROPN
brj-24573	170	13	,	,	PUNCT
brj-24573	170	14	m.	m.	NOUN
brj-24573	170	15	,	,	PUNCT
brj-24573	170	16	ahmad	ahmad	PROPN
brj-24573	170	17	,	,	PUNCT
brj-24573	170	18	n.	n.	PROPN
brj-24573	170	19	,	,	PUNCT
brj-24573	170	20	aslam	aslam	PROPN
brj-24573	170	21	,	,	PUNCT
brj-24573	170	22	m.	m.	NOUN
brj-24573	170	23	,	,	PUNCT
brj-24573	170	24	and	and	CCONJ
brj-24573	170	25	rashid	rashid	PROPN
brj-24573	170	26	,	,	PUNCT
brj-24573	170	27	n.	n.	NOUN
brj-24573	170	28	(	(	PUNCT
brj-24573	170	29	2022	2022	NUM
brj-24573	170	30	)	)	PUNCT
brj-24573	170	31	.	.	PUNCT
brj-24573	171	1	"	"	PUNCT
brj-24573	171	2	investigating	investigate	VERB
brj-24573	171	3	the	the	DET
brj-24573	171	4	role	role	NOUN
brj-24573	171	5	of	of	ADP
brj-24573	171	6	carbohydrate	carbohydrate	NOUN
brj-24573	171	7	-	-	PUNCT
brj-24573	171	8	binding	bind	VERB
brj-24573	171	9	module	module	NOUN
brj-24573	171	10	34	34	NUM
brj-24573	171	11	in	in	ADP
brj-24573	171	12	cyclomaltodextrinase	cyclomaltodextrinase	NOUN
brj-24573	171	13	from	from	ADP
brj-24573	171	14	geobacillus	geobacillus	PROPN
brj-24573	171	15	thermopakistaniensis	thermopakistaniensis	PROPN
brj-24573	171	16	:	:	PUNCT
brj-24573	171	17	structural	structural	ADJ
brj-24573	171	18	and	and	CCONJ
brj-24573	171	19	functional	functional	ADJ
brj-24573	171	20	analyses	analysis	NOUN
brj-24573	171	21	,	,	PUNCT
brj-24573	171	22	"	"	PUNCT
brj-24573	171	23	3	3	NUM
brj-24573	171	24	biotech	biotech	NOUN
brj-24573	171	25	12(25	12(25	NOUN
brj-24573	171	26	)	)	PUNCT
brj-24573	171	27	,	,	PUNCT
brj-24573	171	28	1	1	NUM
brj-24573	171	29	-	-	SYM
brj-24573	171	30	12	12	NUM
brj-24573	171	31	.	.	PUNCT
brj-24573	171	32	doi:10.1007	doi:10.1007	VERB
brj-24573	171	33	/	/	SYM
brj-24573	171	34	s13205	s13205	NOUN
brj-24573	171	35	-	-	PUNCT
brj-24573	171	36	021	021	NUM
brj-24573	171	37	-	-	PUNCT
brj-24573	171	38	03089	03089	NUM
brj-24573	171	39	-	-	SYM
brj-24573	171	40	9	9	NUM
brj-24573	171	41	bradford	bradford	NOUN
brj-24573	171	42	,	,	PUNCT
brj-24573	171	43	m.	m.	NOUN
brj-24573	171	44	m.	m.	NOUN
brj-24573	171	45	(	(	PUNCT
brj-24573	171	46	1976	1976	NUM
brj-24573	171	47	)	)	PUNCT
brj-24573	171	48	.	.	PUNCT
brj-24573	172	1	"	"	PUNCT
brj-24573	172	2	a	a	DET
brj-24573	172	3	rapid	rapid	ADJ
brj-24573	172	4	and	and	CCONJ
brj-24573	172	5	sensitive	sensitive	ADJ
brj-24573	172	6	method	method	NOUN
brj-24573	172	7	for	for	ADP
brj-24573	172	8	the	the	DET
brj-24573	172	9	quantitation	quantitation	NOUN
brj-24573	172	10	of	of	ADP
brj-24573	172	11	microgram	microgram	ADJ
brj-24573	172	12	quantities	quantity	NOUN
brj-24573	172	13	of	of	ADP
brj-24573	172	14	protein	protein	NOUN
brj-24573	172	15	utilizing	utilize	VERB
brj-24573	172	16	the	the	DET
brj-24573	172	17	principle	principle	NOUN
brj-24573	172	18	of	of	ADP
brj-24573	172	19	protein	protein	NOUN
brj-24573	172	20	-	-	PUNCT
brj-24573	172	21	dye	dye	NOUN
brj-24573	172	22	binding	binding	NOUN
brj-24573	172	23	,	,	PUNCT
brj-24573	172	24	"	"	PUNCT
brj-24573	172	25	analytical	analytical	ADJ
brj-24573	172	26	biochemistry	biochemistry	NOUN
brj-24573	172	27	72(1	72(1	NOUN
brj-24573	172	28	-	-	PUNCT
brj-24573	172	29	2	2	NUM
brj-24573	172	30	)	)	PUNCT
brj-24573	172	31	,	,	PUNCT
brj-24573	172	32	248	248	NUM
brj-24573	172	33	-	-	SYM
brj-24573	172	34	254	254	NUM
brj-24573	172	35	.	.	PUNCT
brj-24573	173	1	doi	doi	NOUN
brj-24573	173	2	:	:	PUNCT
brj-24573	173	3	10.1006	10.1006	NUM
brj-24573	173	4	/	/	SYM
brj-24573	173	5	abio.1976.9999	abio.1976.9999	PART
brj-24573	173	6	ferrer	ferrer	NOUN
brj-24573	173	7	-	-	NOUN
brj-24573	173	8	miralles	miralle	NOUN
brj-24573	173	9	,	,	PUNCT
brj-24573	173	10	n.	n.	NOUN
brj-24573	173	11	,	,	PUNCT
brj-24573	173	12	and	and	CCONJ
brj-24573	173	13	villaverde	villaverde	VERB
brj-24573	173	14	,	,	PUNCT
brj-24573	173	15	a.	a.	NOUN
brj-24573	173	16	(	(	PUNCT
brj-24573	173	17	2013	2013	NUM
brj-24573	173	18	)	)	PUNCT
brj-24573	173	19	.	.	PUNCT
brj-24573	174	1	"	"	PUNCT
brj-24573	174	2	bacterial	bacterial	ADJ
brj-24573	174	3	cell	cell	NOUN
brj-24573	174	4	factories	factory	NOUN
brj-24573	174	5	for	for	ADP
brj-24573	174	6	recombinant	recombinant	ADJ
brj-24573	174	7	protein	protein	NOUN
brj-24573	174	8	production	production	NOUN
brj-24573	174	9	;	;	PUNCT
brj-24573	174	10	expanding	expand	VERB
brj-24573	174	11	the	the	DET
brj-24573	174	12	catalogue	catalogue	NOUN
brj-24573	174	13	,	,	PUNCT
brj-24573	174	14	"	"	PUNCT
brj-24573	174	15	in	in	ADP
brj-24573	174	16	:	:	PUNCT
brj-24573	174	17	biomed	biome	VERB
brj-24573	174	18	central	central	ADJ
brj-24573	174	19	,	,	PUNCT
brj-24573	174	20	vol	vol	NOUN
brj-24573	174	21	.	.	PROPN
brj-24573	174	22	12	12	NUM
brj-24573	174	23	,	,	PUNCT
brj-24573	174	24	pp	pp	ADJ
brj-24573	174	25	.	.	PUNCT
brj-24573	175	1	1	1	NUM
brj-24573	175	2	-	-	SYM
brj-24573	175	3	4	4	NUM
brj-24573	175	4	hofemeister	hofemeister	PROPN
brj-24573	175	5	,	,	PUNCT
brj-24573	175	6	b.	b.	PROPN
brj-24573	175	7	,	,	PUNCT
brj-24573	175	8	könig	könig	PROPN
brj-24573	175	9	,	,	PUNCT
brj-24573	175	10	s.	s.	PROPN
brj-24573	175	11	,	,	PUNCT
brj-24573	175	12	hoang	hoang	PROPN
brj-24573	175	13	,	,	PUNCT
brj-24573	175	14	v.	v.	PROPN
brj-24573	175	15	,	,	PUNCT
brj-24573	175	16	engel	engel	PROPN
brj-24573	175	17	,	,	PUNCT
brj-24573	175	18	j.	j.	PROPN
brj-24573	175	19	,	,	PUNCT
brj-24573	175	20	mayer	mayer	PROPN
brj-24573	175	21	,	,	PUNCT
brj-24573	175	22	g.	g.	PROPN
brj-24573	175	23	,	,	PUNCT
brj-24573	175	24	hansen	hansen	PROPN
brj-24573	175	25	,	,	PUNCT
brj-24573	175	26	g.	g.	PROPN
brj-24573	175	27	,	,	PUNCT
brj-24573	175	28	and	and	CCONJ
brj-24573	175	29	hofemeister	hofemeister	PROPN
brj-24573	175	30	,	,	PUNCT
brj-24573	175	31	j.	j.	PROPN
brj-24573	175	32	(	(	PUNCT
brj-24573	175	33	1994	1994	NUM
brj-24573	175	34	)	)	PUNCT
brj-24573	175	35	.	.	PUNCT
brj-24573	176	1	"	"	PUNCT
brj-24573	176	2	the	the	DET
brj-24573	176	3	gene	gene	NOUN
brj-24573	176	4	amye	amye	NOUN
brj-24573	176	5	(	(	PUNCT
brj-24573	176	6	tv1	tv1	PROPN
brj-24573	176	7	)	)	PUNCT
brj-24573	176	8	codes	code	NOUN
brj-24573	176	9	for	for	ADP
brj-24573	176	10	a	a	DET
brj-24573	176	11	nonglucogenic	nonglucogenic	ADJ
brj-24573	176	12	alphaamylase	alphaamylase	NOUN
brj-24573	176	13	from	from	ADP
brj-24573	176	14	thermoactinomyces	thermoactinomyce	NOUN
brj-24573	176	15	vulgaris	vulgaris	NOUN
brj-24573	176	16	94	94	NUM
brj-24573	176	17	-	-	SYM
brj-24573	176	18	2a	2a	NUM
brj-24573	176	19	in	in	ADP
brj-24573	176	20	bacillus	bacillus	NOUN
brj-24573	176	21	subtilis	subtili	NOUN
brj-24573	176	22	,	,	PUNCT
brj-24573	176	23	"	"	PUNCT
brj-24573	176	24	applied	apply	VERB
brj-24573	176	25	environ	environ	PROPN
brj-24573	176	26	.	.	PUNCT
brj-24573	177	1	microbiology	microbiology	NOUN
brj-24573	177	2	60(9	60(9	NUM
brj-24573	177	3	)	)	PUNCT
brj-24573	177	4	,	,	PUNCT
brj-24573	177	5	3381	3381	NUM
brj-24573	177	6	-	-	SYM
brj-24573	177	7	3389	3389	NUM
brj-24573	177	8	.	.	PUNCT
brj-24573	178	1	doi	doi	NOUN
brj-24573	178	2	:	:	PUNCT
brj-24573	178	3	10.1128	10.1128	NUM
brj-24573	178	4	/	/	SYM
brj-24573	178	5	aem.60.9.3381	aem.60.9.3381	NOUN
brj-24573	178	6	-	-	PUNCT
brj-24573	178	7	3389.1994	3389.1994	NUM
brj-24573	178	8	hueck	hueck	NOUN
brj-24573	178	9	,	,	PUNCT
brj-24573	178	10	c.	c.	PROPN
brj-24573	178	11	j.	j.	PROPN
brj-24573	178	12	,	,	PUNCT
brj-24573	178	13	and	and	CCONJ
brj-24573	178	14	hillen	hillen	ADJ
brj-24573	178	15	,	,	PUNCT
brj-24573	178	16	w.	w.	NOUN
brj-24573	178	17	(	(	PUNCT
brj-24573	178	18	1995	1995	NUM
brj-24573	178	19	)	)	PUNCT
brj-24573	178	20	.	.	PUNCT
brj-24573	179	1	"	"	PUNCT
brj-24573	179	2	catabolite	catabolite	ADJ
brj-24573	179	3	repression	repression	NOUN
brj-24573	179	4	in	in	ADP
brj-24573	179	5	bacillus	bacillus	NOUN
brj-24573	179	6	subtilis	subtili	NOUN
brj-24573	179	7	:	:	PUNCT
brj-24573	179	8	a	a	DET
brj-24573	179	9	global	global	ADJ
brj-24573	179	10	regulatory	regulatory	ADJ
brj-24573	179	11	mechanism	mechanism	NOUN
brj-24573	179	12	for	for	ADP
brj-24573	179	13	the	the	DET
brj-24573	179	14	gram‐positive	gram‐positive	ADJ
brj-24573	179	15	bacteria	bacteria	NOUN
brj-24573	179	16	?	?	PUNCT
brj-24573	179	17	,	,	PUNCT
brj-24573	179	18	"	"	PUNCT
brj-24573	179	19	molecular	molecular	ADJ
brj-24573	179	20	microbiology	microbiology	NOUN
brj-24573	179	21	15(3	15(3	NUM
brj-24573	179	22	)	)	PUNCT
brj-24573	179	23	,	,	PUNCT
brj-24573	179	24	395	395	NUM
brj-24573	179	25	-	-	SYM
brj-24573	179	26	401	401	NUM
brj-24573	179	27	.	.	PUNCT
brj-24573	180	1	doi	doi	NOUN
brj-24573	180	2	:	:	PUNCT
brj-24573	180	3	10.1111	10.1111	NUM
brj-24573	180	4	/	/	SYM
brj-24573	180	5	j.1365	j.1365	NOUN
brj-24573	180	6	-	-	PUNCT
brj-24573	180	7	2958.1995.tb02252.x	2958.1995.tb02252.x	NUM
brj-24573	180	8	khlestkin	khlestkin	PROPN
brj-24573	180	9	,	,	PUNCT
brj-24573	180	10	v.	v.	PROPN
brj-24573	180	11	k.	k.	PROPN
brj-24573	180	12	,	,	PUNCT
brj-24573	180	13	peltek	peltek	PROPN
brj-24573	180	14	,	,	PUNCT
brj-24573	180	15	s.	s.	PROPN
brj-24573	180	16	e.	e.	PROPN
brj-24573	180	17	,	,	PUNCT
brj-24573	180	18	and	and	CCONJ
brj-24573	180	19	kolchanov	kolchanov	PROPN
brj-24573	180	20	,	,	PUNCT
brj-24573	180	21	n.	n.	PROPN
brj-24573	180	22	a.	a.	PROPN
brj-24573	180	23	(	(	PUNCT
brj-24573	180	24	2018	2018	NUM
brj-24573	180	25	)	)	PUNCT
brj-24573	180	26	.	.	PUNCT
brj-24573	181	1	"	"	PUNCT
brj-24573	181	2	review	review	NOUN
brj-24573	181	3	of	of	ADP
brj-24573	181	4	direct	direct	ADJ
brj-24573	181	5	chemical	chemical	NOUN
brj-24573	181	6	and	and	CCONJ
brj-24573	181	7	biochemical	biochemical	ADJ
brj-24573	181	8	transformations	transformation	NOUN
brj-24573	181	9	of	of	ADP
brj-24573	181	10	starch	starch	NOUN
brj-24573	181	11	,	,	PUNCT
brj-24573	181	12	"	"	PUNCT
brj-24573	181	13	carbohydrate	carbohydrate	NOUN
brj-24573	181	14	polymers	polymer	NOUN
brj-24573	181	15	181	181	NUM
brj-24573	181	16	,	,	PUNCT
brj-24573	181	17	460	460	NUM
brj-24573	181	18	-	-	SYM
brj-24573	181	19	476	476	NUM
brj-24573	181	20	.	.	PUNCT
brj-24573	182	1	doi	doi	NOUN
brj-24573	182	2	:	:	PUNCT
brj-24573	182	3	10.1016	10.1016	NUM
brj-24573	182	4	/	/	SYM
brj-24573	182	5	j.carbpol.2017.10.035	j.carbpol.2017.10.035	PROPN
brj-24573	182	6	kim	kim	PROPN
brj-24573	182	7	,	,	PUNCT
brj-24573	182	8	l.	l.	PROPN
brj-24573	182	9	,	,	PUNCT
brj-24573	182	10	mogk	mogk	PROPN
brj-24573	182	11	,	,	PUNCT
brj-24573	182	12	a.	a.	NOUN
brj-24573	182	13	,	,	PUNCT
brj-24573	182	14	and	and	CCONJ
brj-24573	182	15	schumann	schumann	PROPN
brj-24573	182	16	,	,	PUNCT
brj-24573	182	17	w.	w.	PROPN
brj-24573	182	18	j.	j.	PROPN
brj-24573	182	19	g.	g.	PROPN
brj-24573	182	20	(	(	PUNCT
brj-24573	182	21	1996	1996	NUM
brj-24573	182	22	)	)	PUNCT
brj-24573	182	23	.	.	PUNCT
brj-24573	183	1	"	"	PUNCT
brj-24573	183	2	a	a	DET
brj-24573	183	3	xylose	xylose	PROPN
brj-24573	183	4	-	-	PUNCT
brj-24573	183	5	inducible	inducible	ADJ
brj-24573	183	6	bacillus	bacillus	NOUN
brj-24573	183	7	subtilis	subtili	NOUN
brj-24573	183	8	integration	integration	NOUN
brj-24573	183	9	vector	vector	NOUN
brj-24573	183	10	and	and	CCONJ
brj-24573	183	11	its	its	PRON
brj-24573	183	12	application	application	NOUN
brj-24573	183	13	,	,	PUNCT
brj-24573	183	14	"	"	PUNCT
brj-24573	183	15	gene	gene	NOUN
brj-24573	183	16	181(1	181(1	NUM
brj-24573	183	17	-	-	SYM
brj-24573	183	18	2	2	NUM
brj-24573	183	19	)	)	PUNCT
brj-24573	183	20	,	,	PUNCT
brj-24573	183	21	71	71	NUM
brj-24573	183	22	-	-	SYM
brj-24573	183	23	76	76	NUM
brj-24573	183	24	.	.	PUNCT
brj-24573	184	1	doi	doi	NOUN
brj-24573	184	2	:	:	PUNCT
brj-24573	184	3	10.1016	10.1016	NUM
brj-24573	184	4	/	/	SYM
brj-24573	184	5	s03781119(96)00466	s03781119(96)00466	VERB
brj-24573	184	6	-	-	PUNCT
brj-24573	184	7	0	0	NUM
brj-24573	184	8	kumar	kumar	PROPN
brj-24573	184	9	,	,	PUNCT
brj-24573	184	10	p.	p.	PROPN
brj-24573	184	11	,	,	PUNCT
brj-24573	184	12	patel	patel	PROPN
brj-24573	184	13	,	,	PUNCT
brj-24573	184	14	s.	s.	PROPN
brj-24573	184	15	k.	k.	PROPN
brj-24573	184	16	,	,	PUNCT
brj-24573	184	17	lee	lee	PROPN
brj-24573	184	18	,	,	PUNCT
brj-24573	184	19	j.-k	j.-k	PROPN
brj-24573	184	20	.	.	PUNCT
brj-24573	184	21	,	,	PUNCT
brj-24573	184	22	and	and	CCONJ
brj-24573	184	23	kalia	kalia	NOUN
brj-24573	184	24	,	,	PUNCT
brj-24573	184	25	v.	v.	CCONJ
brj-24573	184	26	c.	c.	PROPN
brj-24573	184	27	(	(	PUNCT
brj-24573	184	28	2013	2013	NUM
brj-24573	184	29	)	)	PUNCT
brj-24573	184	30	.	.	PUNCT
brj-24573	185	1	"	"	PUNCT
brj-24573	185	2	extending	extend	VERB
brj-24573	185	3	the	the	DET
brj-24573	185	4	limits	limit	NOUN
brj-24573	185	5	of	of	ADP
brj-24573	185	6	bacillus	bacillus	NOUN
brj-24573	185	7	for	for	ADP
brj-24573	185	8	novel	novel	ADJ
brj-24573	185	9	biotechnological	biotechnological	ADJ
brj-24573	185	10	applications	application	NOUN
brj-24573	185	11	,	,	PUNCT
brj-24573	185	12	"	"	PUNCT
brj-24573	185	13	biotechnology	biotechnology	NOUN
brj-24573	185	14	advances	advance	VERB
brj-24573	185	15	31(8	31(8	NUM
brj-24573	185	16	)	)	PUNCT
brj-24573	185	17	,	,	PUNCT
brj-24573	185	18	1543	1543	NUM
brj-24573	185	19	-	-	SYM
brj-24573	185	20	1561	1561	NUM
brj-24573	185	21	.	.	PUNCT
brj-24573	186	1	doi	doi	NOUN
brj-24573	186	2	:	:	PUNCT
brj-24573	186	3	10.1016	10.1016	NUM
brj-24573	186	4	/	/	SYM
brj-24573	186	5	j.biotechadv.2013.08.007	j.biotechadv.2013.08.007	PROPN
brj-24573	186	6	liu	liu	PROPN
brj-24573	186	7	,	,	PUNCT
brj-24573	186	8	z.	z.	PROPN
brj-24573	186	9	,	,	PUNCT
brj-24573	186	10	wang	wang	PROPN
brj-24573	186	11	,	,	PUNCT
brj-24573	186	12	y.	y.	PROPN
brj-24573	186	13	,	,	PUNCT
brj-24573	186	14	liu	liu	PROPN
brj-24573	186	15	,	,	PUNCT
brj-24573	186	16	s.	s.	PROPN
brj-24573	186	17	,	,	PUNCT
brj-24573	186	18	guo	guo	PROPN
brj-24573	186	19	,	,	PUNCT
brj-24573	186	20	x.	x.	PROPN
brj-24573	186	21	,	,	PUNCT
brj-24573	186	22	zhao	zhao	PROPN
brj-24573	186	23	,	,	PUNCT
brj-24573	186	24	t.	t.	PROPN
brj-24573	186	25	,	,	PUNCT
brj-24573	186	26	wu	wu	PROPN
brj-24573	186	27	,	,	PUNCT
brj-24573	186	28	j.	j.	PROPN
brj-24573	186	29	,	,	PUNCT
brj-24573	186	30	and	and	CCONJ
brj-24573	186	31	chen	chen	PROPN
brj-24573	186	32	,	,	PUNCT
brj-24573	186	33	s.	s.	PROPN
brj-24573	186	34	(	(	PUNCT
brj-24573	186	35	2022	2022	NUM
brj-24573	186	36	)	)	PUNCT
brj-24573	186	37	.	.	PUNCT
brj-24573	187	1	"	"	PUNCT
brj-24573	187	2	boosting	boost	VERB
brj-24573	187	3	the	the	DET
brj-24573	187	4	heterologous	heterologous	ADJ
brj-24573	187	5	expression	expression	NOUN
brj-24573	187	6	of	of	ADP
brj-24573	187	7	d	d	X
brj-24573	187	8	-	-	PUNCT
brj-24573	187	9	allulose	allulose	ADJ
brj-24573	187	10	3	3	NUM
brj-24573	187	11	-	-	NOUN
brj-24573	187	12	epimerase	epimerase	NOUN
brj-24573	187	13	in	in	ADP
brj-24573	187	14	bacillus	bacillus	NOUN
brj-24573	187	15	escherlis	escherli	NOUN
brj-24573	187	16	through	through	ADP
brj-24573	187	17	protein	protein	NOUN
brj-24573	187	18	engineering	engineering	NOUN
brj-24573	187	19	and	and	CCONJ
brj-24573	187	20	catabolite	catabolite	ADJ
brj-24573	187	21	-	-	PUNCT
brj-24573	187	22	responsive	responsive	ADJ
brj-24573	187	23	element	element	NOUN
brj-24573	187	24	box	box	PROPN
brj-24573	187	25	engineering	engineering	NOUN
brj-24573	187	26	,	,	PUNCT
brj-24573	187	27	"	"	PUNCT
brj-24573	187	28	journal	journal	NOUN
brj-24573	187	29	of	of	ADP
brj-24573	187	30	agricultural	agricultural	ADJ
brj-24573	187	31	food	food	NOUN
brj-24573	187	32	chemistry	chemistry	NOUN
brj-24573	187	33	70(38	70(38	NUM
brj-24573	187	34	)	)	PUNCT
brj-24573	187	35	,	,	PUNCT
brj-24573	187	36	12128	12128	NUM
brj-24573	187	37	-	-	SYM
brj-24573	187	38	12134	12134	NUM
brj-24573	187	39	.	.	PUNCT
brj-24573	188	1	doi	doi	NOUN
brj-24573	188	2	:	:	PUNCT
brj-24573	188	3	10.1021	10.1021	NUM
brj-24573	188	4	/	/	SYM
brj-24573	188	5	acs.jafc.2c04800	acs.jafc.2c04800	NOUN
brj-24573	188	6	miller	miller	NOUN
brj-24573	188	7	,	,	PUNCT
brj-24573	188	8	g.	g.	PROPN
brj-24573	188	9	l.	l.	PROPN
brj-24573	188	10	(	(	PUNCT
brj-24573	188	11	1959	1959	NUM
brj-24573	188	12	)	)	PUNCT
brj-24573	188	13	.	.	PUNCT
brj-24573	189	1	"	"	PUNCT
brj-24573	189	2	use	use	NOUN
brj-24573	189	3	of	of	ADP
brj-24573	189	4	dinitrosalicylic	dinitrosalicylic	ADJ
brj-24573	189	5	acid	acid	NOUN
brj-24573	189	6	reagent	reagent	NOUN
brj-24573	189	7	for	for	ADP
brj-24573	189	8	determination	determination	NOUN
brj-24573	189	9	of	of	ADP
brj-24573	189	10	reducing	reduce	VERB
brj-24573	189	11	sugar	sugar	NOUN
brj-24573	189	12	,	,	PUNCT
brj-24573	189	13	"	"	PUNCT
brj-24573	189	14	analytical	analytical	ADJ
brj-24573	189	15	chemistry	chemistry	NOUN
brj-24573	189	16	31(3	31(3	NUM
brj-24573	189	17	)	)	PUNCT
brj-24573	189	18	,	,	PUNCT
brj-24573	189	19	426	426	NUM
brj-24573	189	20	-	-	SYM
brj-24573	189	21	428	428	NUM
brj-24573	189	22	.	.	PUNCT
brj-24573	189	23	doi	doi	NOUN
brj-24573	189	24	:	:	PUNCT
brj-24573	189	25	10.1021	10.1021	NUM
brj-24573	189	26	/	/	SYM
brj-24573	189	27	ac60147a030	ac60147a030	PROPN
brj-24573	189	28	https://doi.org/10.1515/amylase-2021-0003	https://doi.org/10.1515/amylase-2021-0003	PROPN
brj-24573	189	29	https://doi.org/10.1006/abio.1976.9999	https://doi.org/10.1006/abio.1976.9999	PROPN
brj-24573	189	30	https://doi.org/10.1128/aem.60.9.3381-3389.1994	https://doi.org/10.1128/aem.60.9.3381-3389.1994	PROPN
brj-24573	189	31	https://doi.org/10.1111/j.1365-2958.1995.tb02252.x	https://doi.org/10.1111/j.1365-2958.1995.tb02252.x	PROPN
brj-24573	189	32	https://doi.org/10.1016/j.carbpol.2017.10.035	https://doi.org/10.1016/j.carbpol.2017.10.035	VERB
brj-24573	189	33	https://doi.org/10.1016/s0378-1119(96)00466-0	https://doi.org/10.1016/s0378-1119(96)00466-0	ADJ
brj-24573	189	34	https://doi.org/10.1016/s0378-1119(96)00466-0	https://doi.org/10.1016/s0378-1119(96)00466-0	ADJ
brj-24573	189	35	https://doi.org/10.1016/j.biotechadv.2013.08.007	https://doi.org/10.1016/j.biotechadv.2013.08.007	ADJ
brj-24573	189	36	https://doi.org/10.1021/acs.jafc.2c04800	https://doi.org/10.1021/acs.jafc.2c04800	NOUN
brj-24573	189	37	https://doi.org/10.1021/ac60147a030	https://doi.org/10.1021/ac60147a030	PROPN
brj-24573	189	38	peer	peer	NOUN
brj-24573	189	39	-	-	PUNCT
brj-24573	189	40	reviewed	review	VERB
brj-24573	189	41	article	article	NOUN
brj-24573	189	42	bioresources.cnr.ncsu.edu	bioresources.cnr.ncsu.edu	X
brj-24573	189	43	farouk	farouk	PROPN
brj-24573	189	44	et	et	PROPN
brj-24573	189	45	al	al	PROPN
brj-24573	189	46	.	.	PROPN
brj-24573	190	1	(	(	PUNCT
brj-24573	190	2	2025	2025	NUM
brj-24573	190	3	)	)	PUNCT
brj-24573	190	4	.	.	PUNCT
brj-24573	191	1	“	"	PUNCT
brj-24573	191	2	amylase	amylase	NOUN
brj-24573	191	3	via	via	ADP
brj-24573	191	4	xylose	xylose	PROPN
brj-24573	191	5	promoter	promoter	NOUN
brj-24573	191	6	,	,	PUNCT
brj-24573	191	7	”	"	PUNCT
brj-24573	191	8	bioresources	bioresource	NOUN
brj-24573	191	9	20(3	20(3	NOUN
brj-24573	191	10	)	)	PUNCT
brj-24573	191	11	,	,	PUNCT
brj-24573	191	12	7728	7728	NUM
brj-24573	191	13	-	-	SYM
brj-24573	191	14	7737	7737	NUM
brj-24573	191	15	.	.	PUNCT
brj-24573	191	16	7737	7737	NUM
brj-24573	191	17	nair	nair	NOUN
brj-24573	191	18	,	,	PUNCT
brj-24573	191	19	a.	a.	NOUN
brj-24573	191	20	,	,	PUNCT
brj-24573	191	21	and	and	CCONJ
brj-24573	191	22	sarma	sarma	PROPN
brj-24573	191	23	,	,	PUNCT
brj-24573	191	24	s.	s.	PROPN
brj-24573	191	25	j.	j.	PROPN
brj-24573	191	26	(	(	PUNCT
brj-24573	191	27	2021	2021	NUM
brj-24573	191	28	)	)	PUNCT
brj-24573	191	29	.	.	PUNCT
brj-24573	192	1	"	"	PUNCT
brj-24573	192	2	the	the	DET
brj-24573	192	3	impact	impact	NOUN
brj-24573	192	4	of	of	ADP
brj-24573	192	5	carbon	carbon	NOUN
brj-24573	192	6	and	and	CCONJ
brj-24573	192	7	nitrogen	nitrogen	NOUN
brj-24573	192	8	catabolite	catabolite	ADJ
brj-24573	192	9	repression	repression	NOUN
brj-24573	192	10	in	in	ADP
brj-24573	192	11	microorganisms	microorganism	NOUN
brj-24573	192	12	,	,	PUNCT
brj-24573	192	13	"	"	PUNCT
brj-24573	192	14	microbiological	microbiological	ADJ
brj-24573	192	15	research	research	NOUN
brj-24573	192	16	251	251	NUM
brj-24573	192	17	,	,	PUNCT
brj-24573	192	18	article	article	NOUN
brj-24573	192	19	126831	126831	NUM
brj-24573	192	20	.	.	PUNCT
brj-24573	193	1	doi	doi	NOUN
brj-24573	193	2	:	:	PUNCT
brj-24573	193	3	10.1016	10.1016	NUM
brj-24573	193	4	/	/	SYM
brj-24573	193	5	j.micres.2021.126831	j.micres.2021.126831	PROPN
brj-24573	193	6	pereira	pereira	PROPN
brj-24573	193	7	,	,	PUNCT
brj-24573	193	8	y.	y.	PROPN
brj-24573	193	9	,	,	PUNCT
brj-24573	193	10	petit	petit	NOUN
brj-24573	193	11	-	-	PUNCT
brj-24573	193	12	glatron	glatron	NOUN
brj-24573	193	13	,	,	PUNCT
brj-24573	193	14	m.-f	m.-f	NOUN
brj-24573	193	15	.	.	PUNCT
brj-24573	193	16	,	,	PUNCT
brj-24573	193	17	and	and	CCONJ
brj-24573	193	18	chambert	chambert	PROPN
brj-24573	193	19	,	,	PUNCT
brj-24573	193	20	r.	r.	PROPN
brj-24573	193	21	(	(	PUNCT
brj-24573	193	22	2001	2001	NUM
brj-24573	193	23	)	)	PUNCT
brj-24573	193	24	.	.	PUNCT
brj-24573	194	1	"	"	PUNCT
brj-24573	194	2	yveb	yveb	NOUN
brj-24573	194	3	,	,	PUNCT
brj-24573	194	4	encoding	encode	VERB
brj-24573	194	5	endolevanase	endolevanase	NOUN
brj-24573	194	6	levb	levb	PROPN
brj-24573	194	7	,	,	PUNCT
brj-24573	194	8	is	be	AUX
brj-24573	194	9	part	part	NOUN
brj-24573	194	10	of	of	ADP
brj-24573	194	11	the	the	DET
brj-24573	194	12	sacb	sacb	NOUN
brj-24573	194	13	–	–	PUNCT
brj-24573	194	14	yveb	yveb	NOUN
brj-24573	194	15	–	–	PUNCT
brj-24573	194	16	yvea	yvea	PROPN
brj-24573	194	17	levansucrase	levansucrase	NOUN
brj-24573	194	18	tricistronic	tricistronic	PROPN
brj-24573	194	19	operon	operon	PROPN
brj-24573	194	20	in	in	ADP
brj-24573	194	21	bacillus	bacillus	NOUN
brj-24573	194	22	subtilis	subtili	NOUN
brj-24573	194	23	,	,	PUNCT
brj-24573	194	24	"	"	PUNCT
brj-24573	194	25	microbiology	microbiology	NOUN
brj-24573	194	26	147(12	147(12	NUM
brj-24573	194	27	)	)	PUNCT
brj-24573	194	28	,	,	PUNCT
brj-24573	194	29	3413	3413	NUM
brj-24573	194	30	-	-	SYM
brj-24573	194	31	3419	3419	NUM
brj-24573	194	32	.	.	PUNCT
brj-24573	195	1	doi	doi	NOUN
brj-24573	195	2	:	:	PUNCT
brj-24573	195	3	10.1099/00221287	10.1099/00221287	NUM
brj-24573	195	4	-	-	SYM
brj-24573	195	5	147	147	NUM
brj-24573	195	6	-	-	PUNCT
brj-24573	195	7	12	12	NUM
brj-24573	195	8	-	-	PUNCT
brj-24573	195	9	3413	3413	NUM
brj-24573	195	10	reizer	reizer	NOUN
brj-24573	195	11	,	,	PUNCT
brj-24573	195	12	a.	a.	NOUN
brj-24573	195	13	,	,	PUNCT
brj-24573	195	14	deutscher	deutscher	PROPN
brj-24573	195	15	,	,	PUNCT
brj-24573	195	16	j.	j.	PROPN
brj-24573	195	17	,	,	PUNCT
brj-24573	195	18	saier	saier	PROPN
brj-24573	195	19	jr	jr	PROPN
brj-24573	195	20	,	,	PUNCT
brj-24573	195	21	m.	m.	NOUN
brj-24573	195	22	,	,	PUNCT
brj-24573	195	23	and	and	CCONJ
brj-24573	195	24	reizer	reizer	NOUN
brj-24573	195	25	,	,	PUNCT
brj-24573	195	26	j.	j.	PROPN
brj-24573	195	27	(	(	PUNCT
brj-24573	195	28	1991	1991	NUM
brj-24573	195	29	)	)	PUNCT
brj-24573	195	30	.	.	PUNCT
brj-24573	196	1	"	"	PUNCT
brj-24573	196	2	analysis	analysis	NOUN
brj-24573	196	3	of	of	ADP
brj-24573	196	4	the	the	DET
brj-24573	196	5	gluconate	gluconate	NOUN
brj-24573	196	6	(	(	PUNCT
brj-24573	196	7	gnt	gnt	PROPN
brj-24573	196	8	)	)	PUNCT
brj-24573	196	9	operon	operon	NOUN
brj-24573	196	10	of	of	ADP
brj-24573	196	11	bacillus	bacillus	NOUN
brj-24573	196	12	subtilis	subtili	NOUN
brj-24573	196	13	,	,	PUNCT
brj-24573	196	14	"	"	PUNCT
brj-24573	196	15	molecular	molecular	ADJ
brj-24573	196	16	microbiology	microbiology	NOUN
brj-24573	196	17	5(5	5(5	NUM
brj-24573	196	18	)	)	PUNCT
brj-24573	196	19	,	,	PUNCT
brj-24573	196	20	1081	1081	NUM
brj-24573	196	21	-	-	SYM
brj-24573	196	22	1089	1089	NUM
brj-24573	196	23	.	.	PUNCT
brj-24573	197	1	doi	doi	NOUN
brj-24573	197	2	:	:	PUNCT
brj-24573	197	3	10.1111	10.1111	NUM
brj-24573	197	4	/	/	SYM
brj-24573	197	5	j.1365	j.1365	NOUN
brj-24573	197	6	-	-	PUNCT
brj-24573	197	7	2958.1991.tb01880.x	2958.1991.tb01880.x	NUM
brj-24573	197	8	rygus	rygus	NOUN
brj-24573	197	9	,	,	PUNCT
brj-24573	197	10	t.	t.	NOUN
brj-24573	197	11	,	,	PUNCT
brj-24573	197	12	and	and	CCONJ
brj-24573	197	13	hillen	hillen	ADJ
brj-24573	197	14	,	,	PUNCT
brj-24573	197	15	w.	w.	NOUN
brj-24573	197	16	(	(	PUNCT
brj-24573	197	17	1991a	1991a	NUM
brj-24573	197	18	)	)	PUNCT
brj-24573	197	19	.	.	PUNCT
brj-24573	198	1	"	"	PUNCT
brj-24573	198	2	inducible	inducible	ADJ
brj-24573	198	3	high	high	ADJ
brj-24573	198	4	-	-	PUNCT
brj-24573	198	5	level	level	NOUN
brj-24573	198	6	expression	expression	NOUN
brj-24573	198	7	of	of	ADP
brj-24573	198	8	heterologous	heterologous	ADJ
brj-24573	198	9	genes	gene	NOUN
brj-24573	198	10	in	in	ADP
brj-24573	198	11	bacillus	bacillus	NOUN
brj-24573	198	12	megaterium	megaterium	NOUN
brj-24573	198	13	using	use	VERB
brj-24573	198	14	the	the	DET
brj-24573	198	15	regulatory	regulatory	ADJ
brj-24573	198	16	elements	element	NOUN
brj-24573	198	17	of	of	ADP
brj-24573	198	18	the	the	DET
brj-24573	198	19	xylose	xylose	PROPN
brj-24573	198	20	-	-	PUNCT
brj-24573	198	21	utilization	utilization	NOUN
brj-24573	198	22	operon	operon	NOUN
brj-24573	198	23	,	,	PUNCT
brj-24573	198	24	"	"	PUNCT
brj-24573	198	25	applied	apply	VERB
brj-24573	198	26	microbiology	microbiology	NOUN
brj-24573	198	27	and	and	CCONJ
brj-24573	198	28	biotechnology	biotechnology	NOUN
brj-24573	198	29	35	35	NUM
brj-24573	198	30	,	,	PUNCT
brj-24573	198	31	594	594	NUM
brj-24573	198	32	-	-	SYM
brj-24573	198	33	599	599	NUM
brj-24573	198	34	.	.	PUNCT
brj-24573	199	1	doi	doi	NOUN
brj-24573	199	2	:	:	PUNCT
brj-24573	199	3	10.1007	10.1007	NUM
brj-24573	199	4	/	/	SYM
brj-24573	199	5	bf00169622	bf00169622	PROPN
brj-24573	199	6	rygus	rygus	PROPN
brj-24573	199	7	,	,	PUNCT
brj-24573	199	8	t.	t.	NOUN
brj-24573	199	9	,	,	PUNCT
brj-24573	199	10	and	and	CCONJ
brj-24573	199	11	hillen	hillen	ADJ
brj-24573	199	12	,	,	PUNCT
brj-24573	199	13	w.	w.	NOUN
brj-24573	199	14	(	(	PUNCT
brj-24573	199	15	1991b	1991b	NUM
brj-24573	199	16	)	)	PUNCT
brj-24573	199	17	.	.	PUNCT
brj-24573	200	1	"	"	PUNCT
brj-24573	200	2	inducible	inducible	ADJ
brj-24573	200	3	high	high	ADJ
brj-24573	200	4	-	-	PUNCT
brj-24573	200	5	level	level	NOUN
brj-24573	200	6	expression	expression	NOUN
brj-24573	200	7	of	of	ADP
brj-24573	200	8	heterologous	heterologous	ADJ
brj-24573	200	9	genes	gene	NOUN
brj-24573	200	10	in	in	ADP
brj-24573	200	11	bacillus	bacillus	NOUN
brj-24573	200	12	megaterium	megaterium	NOUN
brj-24573	200	13	using	use	VERB
brj-24573	200	14	the	the	DET
brj-24573	200	15	regulatory	regulatory	ADJ
brj-24573	200	16	elements	element	NOUN
brj-24573	200	17	of	of	ADP
brj-24573	200	18	the	the	DET
brj-24573	200	19	xylose	xylose	PROPN
brj-24573	200	20	-	-	PUNCT
brj-24573	200	21	utilization	utilization	NOUN
brj-24573	200	22	operon	operon	NOUN
brj-24573	200	23	,	,	PUNCT
brj-24573	200	24	"	"	PUNCT
brj-24573	200	25	applied	apply	VERB
brj-24573	200	26	microbiology	microbiology	NOUN
brj-24573	200	27	and	and	CCONJ
brj-24573	200	28	biotechnology	biotechnology	NOUN
brj-24573	200	29	,	,	PUNCT
brj-24573	200	30	"	"	PUNCT
brj-24573	200	31	35	35	NUM
brj-24573	200	32	,	,	PUNCT
brj-24573	200	33	594	594	NUM
brj-24573	200	34	-	-	SYM
brj-24573	200	35	599	599	NUM
brj-24573	200	36	.	.	PUNCT
brj-24573	201	1	rygus	rygus	PROPN
brj-24573	201	2	,	,	PUNCT
brj-24573	201	3	t.	t.	NOUN
brj-24573	201	4	,	,	PUNCT
brj-24573	201	5	and	and	CCONJ
brj-24573	201	6	hillen	hillen	ADJ
brj-24573	201	7	,	,	PUNCT
brj-24573	201	8	w.	w.	NOUN
brj-24573	201	9	(	(	PUNCT
brj-24573	201	10	1992	1992	NUM
brj-24573	201	11	)	)	PUNCT
brj-24573	201	12	.	.	PUNCT
brj-24573	202	1	"	"	PUNCT
brj-24573	202	2	catabolite	catabolite	ADJ
brj-24573	202	3	repression	repression	NOUN
brj-24573	202	4	of	of	ADP
brj-24573	202	5	the	the	DET
brj-24573	202	6	xyl	xyl	PROPN
brj-24573	202	7	operon	operon	PROPN
brj-24573	202	8	in	in	ADP
brj-24573	202	9	bacillus	bacillus	NOUN
brj-24573	202	10	megaterium	megaterium	NOUN
brj-24573	202	11	,	,	PUNCT
brj-24573	202	12	"	"	PUNCT
brj-24573	202	13	journal	journal	NOUN
brj-24573	202	14	of	of	ADP
brj-24573	202	15	bacteriology	bacteriology	NOUN
brj-24573	202	16	174(9	174(9	NUM
brj-24573	202	17	)	)	PUNCT
brj-24573	202	18	,	,	PUNCT
brj-24573	202	19	3049	3049	NUM
brj-24573	202	20	-	-	SYM
brj-24573	202	21	3055	3055	NUM
brj-24573	202	22	.	.	PUNCT
brj-24573	203	1	doi	doi	NOUN
brj-24573	203	2	:	:	PUNCT
brj-24573	203	3	10.1128	10.1128	NUM
brj-24573	203	4	/	/	SYM
brj-24573	203	5	jb.174.9.3049	jb.174.9.3049	NOUN
brj-24573	203	6	-	-	PUNCT
brj-24573	203	7	3055.1992	3055.1992	NUM
brj-24573	203	8	terpe	terpe	NOUN
brj-24573	203	9	,	,	PUNCT
brj-24573	203	10	k.	k.	PROPN
brj-24573	203	11	(	(	PUNCT
brj-24573	203	12	2006	2006	NUM
brj-24573	203	13	)	)	PUNCT
brj-24573	203	14	.	.	PUNCT
brj-24573	204	1	"	"	PUNCT
brj-24573	204	2	overview	overview	NOUN
brj-24573	204	3	of	of	ADP
brj-24573	204	4	bacterial	bacterial	ADJ
brj-24573	204	5	expression	expression	NOUN
brj-24573	204	6	systems	system	NOUN
brj-24573	204	7	for	for	ADP
brj-24573	204	8	heterologous	heterologous	ADJ
brj-24573	204	9	protein	protein	NOUN
brj-24573	204	10	production	production	NOUN
brj-24573	204	11	:	:	PUNCT
brj-24573	204	12	from	from	ADP
brj-24573	204	13	molecular	molecular	ADJ
brj-24573	204	14	and	and	CCONJ
brj-24573	204	15	biochemical	biochemical	ADJ
brj-24573	204	16	fundamentals	fundamental	NOUN
brj-24573	204	17	to	to	ADP
brj-24573	204	18	commercial	commercial	ADJ
brj-24573	204	19	systems	system	NOUN
brj-24573	204	20	,	,	PUNCT
brj-24573	204	21	"	"	PUNCT
brj-24573	204	22	applied	apply	VERB
brj-24573	204	23	microbiology	microbiology	NOUN
brj-24573	204	24	and	and	CCONJ
brj-24573	204	25	biotech	biotech	NOUN
brj-24573	204	26	.	.	PUNCT
brj-24573	205	1	72	72	NUM
brj-24573	205	2	,	,	PUNCT
brj-24573	205	3	211	211	NUM
brj-24573	205	4	-	-	SYM
brj-24573	205	5	222	222	NUM
brj-24573	205	6	.	.	PUNCT
brj-24573	206	1	doi	doi	NOUN
brj-24573	206	2	:	:	PUNCT
brj-24573	206	3	10.1007	10.1007	NUM
brj-24573	206	4	/	/	SYM
brj-24573	206	5	s00253	s00253	NOUN
brj-24573	206	6	-	-	PUNCT
brj-24573	206	7	006	006	NUM
brj-24573	206	8	-	-	PUNCT
brj-24573	206	9	0465	0465	NUM
brj-24573	206	10	-	-	PUNCT
brj-24573	206	11	8	8	NUM
brj-24573	206	12	van	van	PROPN
brj-24573	206	13	dijl	dijl	NOUN
brj-24573	206	14	,	,	PUNCT
brj-24573	206	15	j.	j.	PROPN
brj-24573	206	16	,	,	PUNCT
brj-24573	206	17	and	and	CCONJ
brj-24573	206	18	hecker	hecker	NOUN
brj-24573	206	19	,	,	PUNCT
brj-24573	206	20	m.	m.	NOUN
brj-24573	206	21	(	(	PUNCT
brj-24573	206	22	2013	2013	NUM
brj-24573	206	23	)	)	PUNCT
brj-24573	206	24	.	.	PUNCT
brj-24573	207	1	"	"	PUNCT
brj-24573	207	2	bacillus	bacillus	NOUN
brj-24573	207	3	subtilis	subtili	NOUN
brj-24573	207	4	:	:	PUNCT
brj-24573	207	5	from	from	ADP
brj-24573	207	6	soil	soil	NOUN
brj-24573	207	7	bacterium	bacterium	NOUN
brj-24573	207	8	to	to	ADP
brj-24573	207	9	supersecreting	supersecreting	ADJ
brj-24573	207	10	cell	cell	NOUN
brj-24573	207	11	factory	factory	NOUN
brj-24573	207	12	,	,	PUNCT
brj-24573	207	13	"	"	PUNCT
brj-24573	207	14	in	in	ADP
brj-24573	207	15	:	:	PUNCT
brj-24573	207	16	microb	microb	VERB
brj-24573	207	17	.	.	PUNCT
brj-24573	208	1	cell	cell	NOUN
brj-24573	208	2	factories	factory	NOUN
brj-24573	208	3	:	:	PUNCT
brj-24573	208	4	biomed	biome	VERB
brj-24573	208	5	central	central	ADJ
brj-24573	208	6	,	,	PUNCT
brj-24573	208	7	vol	vol	NOUN
brj-24573	208	8	.	.	PROPN
brj-24573	208	9	12	12	NUM
brj-24573	208	10	,	,	PUNCT
brj-24573	208	11	pp	pp	ADJ
brj-24573	208	12	.	.	PUNCT
brj-24573	209	1	1	1	NUM
brj-24573	209	2	-	-	SYM
brj-24573	209	3	6	6	NUM
brj-24573	209	4	.	.	PUNCT
brj-24573	210	1	wong	wong	PROPN
brj-24573	210	2	,	,	PUNCT
brj-24573	210	3	s.-l	s.-l	NOUN
brj-24573	210	4	.	.	PUNCT
brj-24573	211	1	(	(	PUNCT
brj-24573	211	2	1995	1995	NUM
brj-24573	211	3	)	)	PUNCT
brj-24573	211	4	.	.	PUNCT
brj-24573	212	1	"	"	PUNCT
brj-24573	212	2	advances	advance	NOUN
brj-24573	212	3	in	in	ADP
brj-24573	212	4	the	the	DET
brj-24573	212	5	use	use	NOUN
brj-24573	212	6	of	of	ADP
brj-24573	212	7	bacillus	bacillus	NOUN
brj-24573	212	8	subtilis	subtili	NOUN
brj-24573	212	9	for	for	ADP
brj-24573	212	10	the	the	DET
brj-24573	212	11	expression	expression	NOUN
brj-24573	212	12	and	and	CCONJ
brj-24573	212	13	secretion	secretion	NOUN
brj-24573	212	14	of	of	ADP
brj-24573	212	15	heterologous	heterologous	ADJ
brj-24573	212	16	proteins	protein	NOUN
brj-24573	212	17	,	,	PUNCT
brj-24573	212	18	"	"	PUNCT
brj-24573	212	19	current	current	ADJ
brj-24573	212	20	opinion	opinion	NOUN
brj-24573	212	21	in	in	ADP
brj-24573	212	22	biotechnology	biotechnology	NOUN
brj-24573	212	23	6(5	6(5	NUM
brj-24573	212	24	)	)	PUNCT
brj-24573	212	25	,	,	PUNCT
brj-24573	212	26	517	517	NUM
brj-24573	212	27	-	-	SYM
brj-24573	212	28	522	522	NUM
brj-24573	212	29	.	.	PUNCT
brj-24573	213	1	doi	doi	NOUN
brj-24573	213	2	:	:	PUNCT
brj-24573	213	3	10.1016/0958	10.1016/0958	NUM
brj-24573	213	4	-	-	PUNCT
brj-24573	213	5	1669(95)80085	1669(95)80085	NUM
brj-24573	213	6	-	-	SYM
brj-24573	213	7	9	9	NUM
brj-24573	213	8	yansura	yansura	NOUN
brj-24573	213	9	,	,	PUNCT
brj-24573	213	10	d.	d.	PROPN
brj-24573	213	11	g.	g.	PROPN
brj-24573	213	12	,	,	PUNCT
brj-24573	213	13	and	and	CCONJ
brj-24573	213	14	henner	henner	PROPN
brj-24573	213	15	,	,	PUNCT
brj-24573	213	16	d.	d.	PROPN
brj-24573	213	17	j.	j.	PROPN
brj-24573	213	18	(	(	PUNCT
brj-24573	213	19	1984	1984	NUM
brj-24573	213	20	)	)	PUNCT
brj-24573	213	21	.	.	PUNCT
brj-24573	214	1	"	"	PUNCT
brj-24573	214	2	use	use	NOUN
brj-24573	214	3	of	of	ADP
brj-24573	214	4	the	the	DET
brj-24573	214	5	escherichia	escherichia	NOUN
brj-24573	214	6	coli	coli	NOUN
brj-24573	214	7	lac	lac	PROPN
brj-24573	214	8	repressor	repressor	NOUN
brj-24573	214	9	and	and	CCONJ
brj-24573	214	10	operator	operator	NOUN
brj-24573	214	11	to	to	PART
brj-24573	214	12	control	control	VERB
brj-24573	214	13	gene	gene	NOUN
brj-24573	214	14	expression	expression	NOUN
brj-24573	214	15	in	in	ADP
brj-24573	214	16	bacillus	bacillus	NOUN
brj-24573	214	17	subtilis	subtili	NOUN
brj-24573	214	18	,	,	PUNCT
brj-24573	214	19	"	"	PUNCT
brj-24573	214	20	proceedings	proceeding	NOUN
brj-24573	214	21	of	of	ADP
brj-24573	214	22	the	the	DET
brj-24573	214	23	national	national	PROPN
brj-24573	214	24	academy	academy	PROPN
brj-24573	214	25	of	of	ADP
brj-24573	214	26	sciences	sciences	PROPN
brj-24573	214	27	81(2	81(2	NUM
brj-24573	214	28	)	)	PUNCT
brj-24573	214	29	,	,	PUNCT
brj-24573	214	30	439	439	NUM
brj-24573	214	31	-	-	SYM
brj-24573	214	32	443	443	NUM
brj-24573	214	33	.	.	PUNCT
brj-24573	215	1	doi	doi	NOUN
brj-24573	215	2	:	:	PUNCT
brj-24573	215	3	10.1073	10.1073	NUM
brj-24573	215	4	/	/	SYM
brj-24573	215	5	pnas.81.2.439	pnas.81.2.439	NOUN
brj-24573	215	6	article	article	NOUN
brj-24573	215	7	submitted	submit	VERB
brj-24573	215	8	:	:	PUNCT
brj-24573	215	9	march	march	PROPN
brj-24573	215	10	10	10	NUM
brj-24573	215	11	,	,	PUNCT
brj-24573	215	12	2025	2025	NUM
brj-24573	215	13	;	;	PUNCT
brj-24573	215	14	peer	peer	NOUN
brj-24573	215	15	review	review	NOUN
brj-24573	215	16	completed	complete	VERB
brj-24573	215	17	:	:	PUNCT
brj-24573	215	18	june	june	PROPN
brj-24573	215	19	29	29	NUM
brj-24573	215	20	,	,	PUNCT
brj-24573	215	21	2025	2025	NUM
brj-24573	215	22	;	;	PUNCT
brj-24573	215	23	revised	revise	VERB
brj-24573	215	24	version	version	NOUN
brj-24573	215	25	received	receive	VERB
brj-24573	215	26	and	and	CCONJ
brj-24573	215	27	accepted	accept	VERB
brj-24573	215	28	:	:	PUNCT
brj-24573	215	29	july	july	PROPN
brj-24573	215	30	1	1	NUM
brj-24573	215	31	,	,	PUNCT
brj-24573	215	32	2025	2025	NUM
brj-24573	215	33	;	;	PUNCT
brj-24573	215	34	published	publish	VERB
brj-24573	215	35	:	:	PUNCT
brj-24573	215	36	july	july	PROPN
brj-24573	215	37	29	29	NUM
brj-24573	215	38	,	,	PUNCT
brj-24573	215	39	2025	2025	NUM
brj-24573	215	40	.	.	PUNCT
brj-24573	216	1	doi	doi	NOUN
brj-24573	216	2	:	:	PUNCT
brj-24573	216	3	10.15376	10.15376	NUM
brj-24573	216	4	/	/	SYM
brj-24573	216	5	biores.20.3.7728	biores.20.3.7728	PROPN
brj-24573	216	6	-	-	PUNCT
brj-24573	216	7	7737	7737	NUM
brj-24573	216	8	https://doi.org/10.1016/j.micres.2021.126831	https://doi.org/10.1016/j.micres.2021.126831	PROPN
brj-24573	216	9	https://doi.org/10.1016/j.micres.2021.126831	https://doi.org/10.1016/j.micres.2021.126831	PROPN
brj-24573	216	10	https://doi.org/10.1099/00221287-147-12-3413	https://doi.org/10.1099/00221287-147-12-3413	VERB
brj-24573	216	11	https://doi.org/10.1111/j.1365-2958.1991.tb01880.x	https://doi.org/10.1111/j.1365-2958.1991.tb01880.x	PROPN
brj-24573	216	12	https://doi.org/10.1111/j.1365-2958.1991.tb01880.x	https://doi.org/10.1111/j.1365-2958.1991.tb01880.x	PROPN
brj-24573	216	13	https://doi.org/10.1007/bf00169622	https://doi.org/10.1007/bf00169622	PART
brj-24573	216	14	https://doi.org/10.1007/bf00169622	https://doi.org/10.1007/bf00169622	PROPN
brj-24573	216	15	https://doi.org/10.1128/jb.174.9.3049-3055.1992	https://doi.org/10.1128/jb.174.9.3049-3055.1992	PROPN
brj-24573	216	16	https://doi.org/10.1128/jb.174.9.3049-3055.1992	https://doi.org/10.1128/jb.174.9.3049-3055.1992	PROPN
brj-24573	216	17	https://doi.org/10.1007/s00253-006-0465-8	https://doi.org/10.1007/s00253-006-0465-8	NUM
brj-24573	216	18	https://doi.org/10.1016/0958-1669(95)80085-9	https://doi.org/10.1016/0958-1669(95)80085-9	PROPN
brj-24573	216	19	https://doi.org/10.1073/pnas.81.2.439	https://doi.org/10.1073/pnas.81.2.439	PROPN