PEER-REVIEW ARTICLE PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6576 Color and Glossiness Properties of Wood Material Surfaces Coated with Aloe Vera Doped Varnish Ahmet Cihangir Yalinkilic * *Corresponding author: acihangir.yalinkilic@dpu.edu.tr DOI: 10.15376/biores.20.3.6577-6598 GRAPHICAL ABSTRACT mailto:acihangir.yalinkilic@dpu.edu.tr https://orcid.org/0000-0003-4442-6233 PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6577 Color and Glossiness Properties of Wood Material Surfaces Coated with Aloe Vera Doped Varnish Ahmet Cihangir Yalinkilic * Surface properties of wood materials were determined before and after coating with cellulosic varnish doped with aloe vera gel. For this purpose, samples were prepared from Anatolian black pine (Pinus nigra subsp. pallasiana), fir (Abies nordmanniana subsp. bornmulleriana Mattf.), Ayous (Triplochiton scleroxylon K. Schum), and Scotch pine (Pinus sylvestris L.) woods in accordance with TS 53 (1981) and TS ISO 3129 (2021) principles and then coated with cellulosic varnishes doped with 0%, 5%, 10% and 15% aloe vera (L.) gel according to ASTM D3023-98 (2017) principles. Color changes according to ASTM D2244-21 (2021) and surface glosses according to TS EN ISO 2813 (2014) were determined on varnished surfaces. As the percentage of aloe vera gel in the varnish increased, the red color tone, yellow color tone, total color change, glossiness perpendicular and parallel to the fibers, and difference values decreased. Aloe vera gel can be preferred as an additive in cellulosic varnish in applications where the objective is no change or minimal change in the color and glossiness values, depending on type of wood and area of use. DOI: 10.15376/biores.20.3.6577-6598 Keywords: Aloe vera gel; Cellulosic varnish; Color change; Glossiness; Wood materials Contact information: Kutahya Dumlupınar University, Department of Industrial Design Engineering, Kütahya, Türkiye; *Corresponding author: acihangir.yalinkilic@dpu.edu.tr INTRODUCTION Wood material has been preferred in interior and exterior decoration for centuries, as it contains various colors and textures. Natural organic, hygroscopic, anisotropic, and heterogeneous wood material fades due to the sun’s rays and cannot withstand external effects for a long time. For this reason, wooden material surfaces are covered with varnishes with protective layer properties. The layer protects the wood against external influences and also increases its aesthetic value. The color, fiber structure, and texture of the varnished wood material become more prominent (Sönmez and Budakcı 2004). The color of wood includes all color differences from white to black. Color is not uniform, even on the same tree. Another reason for the color difference is the different reflection of the rays due to the different intensities. Brightness is the property of reflection of rays coming on the material surface. The brightest surface in wood material is the radial section, where the medullary rays are seen as sheets or bright mirrors. The wood is less shiny in the tangential direction to the annual rings, and it does not show any shine in cross- section. The desired color and brightness can be given by coating the processed wood surface with varnishes (Ors and Keskin 2001). Natural resins with improved properties are used as the main binder or modifying element in varnishes (Sönmez and Budakcı 2004). The melt of the resin-modified mailto:acihangir.yalinkilic@dpu.edu.tr https://orcid.org/0000-0003-4442-6233 PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6578 nitrocellulose in nitrous liquids is called cellulosic varnish. Nitrocellulose is the main binder in the cellulosic system. Resin is an off-white polymer that has completed its formation and has a low solid matter content (Budakcı 2003). Aloe vera (Aloe barbadensis) is a plant belonging to the Liliaceae family and is the most important species of the Aloe genus (Vega-Galvez et al. 2011). The plant consists of succulent green leaves that converge on the stem in the form of a rosette. The leaf of the plant consists of two parts, the green tunic and the gel; it has more than 200 ingredients, such as water and fat-soluble vitamins, minerals, enzymes, polysaccharides, phenolic compounds, and organic acids (Basaran 2020). The gel inside the plant leaf is a liquid extract that has been known as medicine among the people since ancient times. Most scientists have investigated the compounds in the gel. Thousands of poly-mono saccharides with different chemical properties and biological activities are found in the gel. The gel also contains lignin, salicylic acid, saponins, sterols, and triterpenoids. Non-starch polysaccharides and lignin make up 35% of the dry mass of the gel. Fresh aloe gel contains the proteolytic enzyme carboxypeptidase, glutathione peroxidase, and several isoenzymes of superoxide dismutase. Aloe gel contains vitamins, such as A, C, E, B12, thiamin, niacin, folic acid, and minerals, such as Na, K, Ca, Mg, Mn, Zn, Cr, and Fe. Aloe plants contain amino acids lysine, histidine, arginine, cysteine, alanine, methionine, leucine, and isolosine. Today, the gel has commercial uses as a food and beverage additive, a moisturizer in cosmetics, and a wound healer in pharmaceuticals. These effects of the gel are attributed to the high molecular weight components in its content (Güler 2010). In this study, various biofilms were created using AVG (The gel obtained by processing the leaf extract of aloe vera) and SCK (the fruit juice industry sour cherry kernel waste). Some properties of biofilms such as swelling, water holding capacity (WVP), mass loss, corrosion, and biodegradation, etc., were investigated comparatively. As a result, two different variety additives (AVG and SCK) addition and four different weight percent have a significant effect on the biofilms’ swelling percentage, mass loss in water, tensile strength, and tensile elongation (Soydal et al. 2024). It was aimed to determine the effect of addition a polyvinyl alcohol (PVOH) polymer and lactic acid in the presence of aloe vera on the mechanical properties of the wood adhesive. Positive effects were observed on the mechanical properties, compatibility, of the resulting mixture by combining aloe vera, lactic acid with PVOH and wood adhesive (Sweah et al. 2021). Recently, the increasing awareness of being sensitive to the environment and the responsibility it brings has accelerated the determination of natural preservatives with alternative and functional properties that will meet the needs of the wood surface finishing sector by making use of natural and renewable resources. In this study, it was aimed to determine the effect of aloe vera gel on the surface appearance properties of wood when it was applied in increasing percentages to cellulosic sanding sealer and cellulosic glossy varnish. A further goal was to investigate the possibilities of use in the wood surface finishing industry. This study is original in this respect, as aloe vera gel was used for the first time in the field of wood surface finishing. It is thought that the study will make significant contributions to the emergence of new job opportunities. PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6579 EXPERIMENTAL Woods Experiment materials were prepared from Anatolian black pine (Pinus nigra subsp. pallasiana), fir (Abies nordmanniana subsp. bornmulleriana Mattf.), Ayous (Triplochiton scleroxylon K. Schum), and Scotch pine (Pinus sylvestris L.) woods by random selection method from the market. Aloe vera Aloe vera (Aloe barbadensis) samples used in the study were obtained from the producer company located in Konyaaltı district of Antalya Province, which is the only commercial grower of Barbadensis Miller genus seedlings in Turkey (Source: https://www.aloeveraantalya.com/galeri-1.html). The geographic coordinates of the district are shown in Fig. 1. Aloe vera samples that were kept in the refrigerator in accordance with the manufacturer's recommendations were kept in a vertical position for 1 day to remove the yellow colored liquid from the place where the leaves were cut. Then the leaves were divided with a knife into two in the middle and the inner gel part was removed with a spoon. The gel part in solid form was passed through the blender and the foamy mixture was left to rest in the refrigerator for 1 day. The gel, which became liquid in the refrigerator, was filtered and made ready for the experiments. Steps for the preparation of aloe vera gel are shown in Fig. 2. Fig. 1. Aloe vera samples used in the experiments and aloe vera the production facility (Source: https://www.aloeveraantalya.com/galeri-1.html) and the geographic coordinates of the district https://www.aloeveraantalya.com/galeri-1.html https://www.aloeveraantalya.com/galeri-1.html PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6580 Fig. 2. Steps for the preparation of aloe vera gel Cellulosic Varnish A recent trend has been to study the use of nanocellulose particles for the formation of wood finishes. Professionals prefer nitrocellulose varnish finishes and to achieve durable, hard, colorless coatings, especially on furniture (Hubbe and Laleicke 2025). Cellulosic varnishes harden with solvent evaporation and dry physically (Sönmez and Budakcı 2004, Hubbe and Laleicke 2025). Since there is no chemical reaction in the layer formation, it can be applied to any type of wood material surface, provided that it is flawless. The moisture content of the wood material should be at most 12%. Fillers (Css) and final coat (Cgv) varnishes are used in the preparation of a protective layer with cellulosic varnishes. In order for the layer to show sufficient resistance to external effects, PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6581 it is envisaged that 4 coats should be applied and the layer thickness should be at most 200 μm to avoid the risk of cracking (Sönmez and Budakcı 2004). Alkyd resin-based, nitrocellulose modified, air-drying cellulosic sanding sealer and cellulosic glossy varnishes used for varnishing the experiment samples were purchased from the market. Technical information of varnishes is given in Table 1. Table 1. Technical Information of Varnishes Varnish Viscosity (20Cº s/DIN6) Dry Film Thickness (μm) Solids (% by Weight) Number of Layers Proposed Recommended Film Thickness (g/m²) Ctm Ratio (% by Weight) Css 50 to 60 35 to 40 30 ± 2 2 to 3 cross coats 220 to 250 90 to 100 Cgv 40 to 55 35 to 40 32.5 ± 2 2 to 3 cross coats 160 to 180 90 to 100 Css: Cellulosic sanding sealer; Cgv: Cellulosic glossy varnishes; Ctm: Cellulosic thinner mixing (Source: https://tdsapp.polisankansai.com/en/pdf/view/61320cab8db9030010d872f4/en/webpage, https://tdsapp.polisankansai.com/en/pdf/view/5a9e3e44b7e0d5e54020f073/en/webpage) Preparation of Test Samples Samples were prepared from the sapwood part of randomly selected first class material that had uniform fiber, did not have knots or cracks, and had no color and density difference, and the annual rings were upright to the surface (TS 53 1981; TS ISO 3129 2021). Samples were cut to 11 × 11 × 1.2 cm3, and they were kept according to TS ISO 13061-1 (2021) principle, under the conditions of 20 ± 2 °C temperature and 50 ± 5% relative humidity until they reached constant weight. Average moisture of 12 randomly chosen samples was determined as 9 ± 0.5%. After samples had been sawn into their final sizes (10 × 10 × 1 cm3) were sanded with No. 80 and No. 100 sandpapers following the first wetting, then they were sanded with No. 150 sandpaper following the final wetting. Sanded surfaces were cleaned from dust using a soft bristle brush and vacuum and made ready for varnishing before surface treatment. In the research, (4 × 4 × 1 × 1 × 12) = 192 samples in total were prepared for the surface experiments. The trial pattern for the surface experiments is shown in Table 2, and images samples experiments are given in Fig. 3. Table 2. The Trial Pattern for the Surface Experiments Wood species Varnish type Color measurement treatment Gloss measurement treatment The number of samples (for each) Total Anatolian black pine V and 0% Av 1 1 12 48 Fir V and 5% Av 1 1 12 48 Ayous V and 10% Av 1 1 12 48 Scots pine V and 15% Av 1 1 12 48 4 4 1 1 12 192 Varnishing Process Varnishing of the samples was carried out to match the industrial application according to ASTM D3023-98 (2017) principles and proposals of producing companies. Varnishes were applied to experiment samples with a medium brush. The amount of varnish was determined by conforming to the proposals of producing companies for two PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6582 cross coats that are required to be applied, and by scaling with analytical and 0.01 g precision balance in a way that there are 220 g per m2 in cellulosic sanding sealer and 160 g per m2 in cellulosic glossy varnish. The amount of mixture applied to the experiment samples by adding 0%, 5%, 10%, and 15% aloe vera gel by weight into cellulosic sanding sealer and cellulosic glossy varnish are given in Table 3. Control V and 5% Av V and 10% Av V and 15% Av Fig. 3. Images samples experiments PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6583 Table 3. Amount of Mixture Applied to the Experiment Samples (g/m2) Varnish 0% Aloe vera 5% Aloe vera 10% Aloe vera 15% Aloe vera Css 220 231 242 253 Cgv 160 168 176 184 Samples over which cellulosic sanding sealer was applied in two cross coats were kept drying for 24 h in a dust-free environment at room temperature in parallel with the ground plane. Dried samples were sanded with 220 and 400 water sandpaper equally, and varnish dusts were cleaned with a soft hairbrush and vacuum method. Cellulosic glossy varnish was applied in two cross coats to the surfaces of experiment samples and kept drying for 3 weeks. After drying, it was kept in an air-conditioning cabinet at 23 ± 2 ºC and 50 ± 5% relative humidity for 16 h according to ASTM D3924-16 (2019) principles before the experiments. The pH values of aloe vera and varnishes on universal indicator strips are shown in Table 4, and images before and after mixing are given in Fig. 4. It has been reported in the literature that the pH of aloe vera gel grown in the field is between 4.4 to 4.7 and the high acidity of aloe vera gel may be due to the accumulation of organic acids, such as malic acid, in the cells of the pulp (Boudreau and Beland 2006). Table 4. pH Values of Aloe Vera and Varnishes pH (Universal indicator strips) Aloe vera (Av) 4 Cellulosic sanding sealer (Css) 4 Cellulosic glossy varnish (Cgv) 4 Fig. 4. Aloe vera and varnishes before and after mixing PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6584 Color Measurement The ColorStriker colorimeter measuring according to ASTM D2244-21 (2021) is shown in Fig. 5. The L*a*b* color space (also referred to as CIELAB) is presently one of the most popular color spaces for measuring object color and is widely used in virtually all fields. It is one of the uniform color spaces defined by CIE in 1976. In this color space, L* indicates lightness (L*= 0, black), (L*=100, white) and a* and b* are the chromaticity coordinates and c* is chroma, and hº is the hue angle. The value of chroma c* is 0 at the center and increases according to the distance from the center. Hue angle h is defined as starting at the +a* axis and is expressed in (°); 0º would be +a* (red), 90º would be +b* (yellow), 180º would be - a* (green), and 270º would be - b* (blue). CIE L*a*b* color space is shown in Fig. 6 (Konica Minolta 2007). Fig. 5. ColorStriker colorimeter Fig. 6. CIE L*a*b* color space PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6585 To determine the change in yellow color tone (b*), variable quality ∆b* was calculated as (∆b* = Varnished value b* - Unvarnished value b*). When the ∆b* has a low value, it means that the yellow color tone did not change or changed little (Yalınkılıc 2021). To determine the change in red color tone (a*), variable quality ∆a* was calculated as (∆a* = Varnished value a* - Unvarnished value a*). A low ∆a* value means that the red color tone did not change or changed little (Yalınkılıc and Sönmez 2015). In the L*a*b* color space, color difference can be expressed as a single numerical value, ∆E*, which indicates the size of the color difference but not in what way the colors are different. ∆E* is defined by the following equation ∆E*=√(∆L*)²+(∆a*)²+(∆b*)² (Konica Minolta 2007). The ∆E* is in low value and means color tone did not change or changed little (Sögütlü and Sönmez 2006). Surface Glossiness Measurement The glossiness was measured in accordance with the principles of TS EN ISO 2813 (2014) and ASTM D523-14 (2018), which determine the light-reflecting ability of wood material surfaces. For this purpose, measurements were made with a glossiness measuring instrument (micro-TRI-gloss µ) parallel and perpendicular to the fibers of the samples. Micro-TRI-gloss µ and the measuring principle are shown in Fig. 7. Fig. 7. Surface glossiness measuring device (micro-TRI-gloss µ) Data Analysis Measurement differences (varnished value minus unvarnished value) were used as data in evaluating the results. Multiple analysis of variance (MANOVA) was applied to the data in the MSTAT-C statistical evaluation program; when the difference between groups is proven significant, the difference between the average values was compared with the Duncan test. Thus, the order by performance between the factors that were included in the experiment was determined to be divided into homogeneity groups according to the critical value of the least significant difference (LSD). RESULTS AND DISCUSSION Red color tone (Δa*) values, which were obtained by the differences between varnished and unvarnished measurements, were used to determine the interaction of aloe PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6586 vera with cellulosic varnish. Red color values were found different in terms of wood species and varnish and aloe vera mix type. A multi-factor analysis of variance was performed to determine the source of differences, and its results are shown in Table 5. Table 5. Results of Variance Analysis on Change in Red (Δa*) Color Tone Source Degrees of Freedom Sum of Squares Mean Square F Value P ≤ 0.05 Wood Species (A) 3 138.936 46.312 415.5012 0.0000* Varnish and Aloe vera mix type (B) 3 9.863 3.288 29.4952 0.0000* AB 9 3.927 0.436 3.9148 0.0001* Error 176 19.617 0.111 Total 191 172.342 *: Difference is meaningful according to 0.05 According to the results of the variance analysis, wood species and varnish and aloe vera mix type and their mutual interactions were found statistically significant (P ≤ 0.05). Comparison results of Duncan test which was made at the level of wood species and varnish and aloe vera mix type were given in Table 6. Table 6. Comparison Result of Duncan Test Made at the Level of Wood Species and Varnish and Aloe Vera Mix Type Source x̄ HG Wood species Anatolian black pine 0.7052 C Fir 0.7846 C Ayous 2.822 A* Scots pine 1.583 B Varnish and aloe vera mix type Control 1.804 A Varnish and 5% Aloe vera (V and 5% Av) 1.559 B* Varnish and 10% Aloe vera (V and 10% Av) 1.303 C Varnish and 15% Aloe vera (V and 15% Av) 1.229 C LSD: ± 0.1341 *: The highest increase in red color tone; x̄: Arithmetic mean; HG: Homogeneity group; Control: Varnish and 0% Aloe vera (V and 0% Av) According to Table 6, the highest change in red color tone was found in ayous wood materials and (varnish and 5% aloe vera) mix type. The lowest change in red color tone was found in Anatolian black pine and (varnish and 15% aloe vera). Comparison results of Duncan test, which was made at the level of interaction between wood species and varnish and aloe vera mix type, are given in Table 7, and the corresponding graph is shown in Fig. 8. PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6587 Table 7. Duncan Test Comparisons for Wood Species - Varnish and Aloe Vera Mix Type Interaction Wood Varnish Means HG Anatolian black pine Control 1.305 D Varnish and 5% Aloe vera 0.7858 FG Varnish and 10% Aloe vera 0.4233 H Varnish and 15% Aloe vera 0.3067 I** Fir Control 1.001 EF Varnish and 5% Aloe vera 0.7342 FG Varnish and 10% Aloe vera 0.7092 G Varnish and 15% Aloe vera 0.6942 G Ayous Control 3.099 A Varnish and 5% Aloe vera 3.023 A* Varnish and 10% Aloe vera 2.592 B Varnish and 15% Aloe vera 2.572 B Scots pine Control 1.810 C Varnish and 5% Aloe vera 1.719 C Varnish and 10% Aloe vera 1.640 C Varnish and 15% Aloe vera 1.164 DE LSD: ± 0.2683, *: The highest increase in red color tone **: The lowest increase in red color tone According to Table 7, the highest change in red color tone was seen in ayous over which varnish and 5% aloe vera was applied. The lowest change was obtained in Anatolian Black pine over which varnish and 15% aloe vera was applied. It was determined that the red color tone of four tree species decreased as the percentage of aloe vera in the cellulosic varnish increased. It may be recommended to use Anatolian black pine coated with 15% aloe vera added cellulosic varnish in applications where the red color tone does not change or changes little. Indeed, because the ∆a* is in low value means red color tone did not change or changed little, has been reported in the literature. This situation is compatible with the literature (Yalınkılıc and Sönmez 2015). If other wood materials are preferred according to the place of use, it may be recommended to use wood materials coated with 15% aloe vera added cellulosic varnish. Fig. 8. Red color tone (Δa*) variation for wood species - varnish and aloe vera mix type interaction 0 0.5 1 1.5 2 2.5 3 3.5 Control V & 5% Av V & 10% Av V & 15% Av 1 .3 0 5 0 .7 8 5 8 0 .4 2 3 3 0 .3 0 6 71 .0 0 1 0 .7 3 4 2 0 .7 0 9 2 0 .6 9 4 2 3.099 3.023 2.592 2.572 1 .8 1 1 .7 1 9 1 .6 4 1 .1 6 4 R e d c o lo r to n e ( Δ a *) Anatolian black pine Fir Ayous Scots pine PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6588 Yellow color tone (Δb*) values, which were obtained by the differences between varnished and unvarnished measurements, were used to determine the interaction of aloe vera with cellulosic varnish. Yellow color values were found different in terms of wood species and varnish and aloe vera mix type. A multi-factor analysis of variance was performed to determine the source of differences, and its results are shown in Table 8. Table 8. Results of Variance Analysis on Change in Yellow (Δb*) Color Tone Source Degrees of Freedom Sum of Squares Mean Square F Value P ≤ 0.05 Wood Species (A) 3 215.226 71.742 55.1388 0.0000* Varnish and Aloe vera mix type (B) 3 191.410 63.803 49.0373 0.0000* AB 9 43.238 4.804 3.6923 0.0003* Error 176 228.996 1.301 Total 191 678.869 *: Difference is meaningful according to 0.05 According to the results of the variance analysis, wood species and varnish and aloe vera mix type and their mutual interactions were found statistically significant (P ≤ 0.05). Comparison results of Duncan test that was made at the level of wood species and varnish and aloe vera mix type are given in Table 9. Table 9. Comparison Result of Duncan Test Made at the Level of Wood Species and Varnish and Aloe Vera Mix Type Source x̄ HG Wood species Anatolian black pine 9.160 B Fir 9.570 B Ayous 11.12 A* Scots pine 8.185 C Varnish and aloe vera mix type Control 11.01 A Varnish and 5% Aloe vera (V and 5% Av) 9.701 B* Varnish and 10% Aloe vera (V and 10% Av) 9.023 C Varnish and 15% Aloe vera (V and 15% Av) 8.301 D LSD: ± 0.4592 *: The highest increase in yellow color tone; x̄: Arithmetic mean; HG: Homogeneity group; Control: Varnish and 0% Aloe vera (V and 0% Av) According to Table 9, the highest change in yellow color tone was found in ayous wood materials and (varnish and 5% aloe vera) mix type. The lowest change in yellow color tone was found in Scots pine and (varnish and 15% aloe vera). Comparison results of Duncan test that was made at the level of interaction between wood species and varnish and aloe vera mix type are given in Table 10, and the corresponding graph is shown in Fig. 9. PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6589 Table 10. Duncan Test Comparisons for Wood Species – Varnish and Aloe Vera Mix Type Interaction Wood Varnish Means HG Anatolian black pine Control 10.43 BCD Varnish and 5% Aloe vera 9.364 EF Varnish and 10% Aloe vera 8.821 FG Varnish and 15% Aloe vera 8.025 GH Fir Control 9.989 CDE Varnish and 5% Aloe vera 9.929 CDE Varnish and 10% Aloe vera 9.497 EF Varnish and 15% Aloe vera 8.863 FG Ayous Control 12.80 A Varnish and 5% Aloe vera 11.22 B* Varnish and 10% Aloe vera 10.57 BCD Varnish and 15% Aloe vera 9.897 DE Scots pine Control 10.82 BC Varnish and 5% Aloe vera 8.296 G Varnish and 10% Aloe vera 7.200 H Varnish and 15% Aloe vera 6.421 I** LSD: ± 0.9185, *: The highest increase in yellow color tone **: The lowest increase in yellow color tone According to Table 10, the highest change in yellow color tone was seen in ayous over which varnish and 5% aloe vera was applied, whereas the lowest change was obtained in Scots pine over which varnish and 15% aloe vera was applied. It was determined that the yellow color tone of four tree species decreased as the percentage of aloe vera in the cellulosic varnish increased. It may be recommended to use Scots pine coated with 15% aloe vera added cellulosic varnish in applications where the yellow color tone does not change or changes little. Indeed, it has been reported that because ∆b* is a low value the yellow color tone did not change or changed little. This situation is compatible with the literature (Yalınkılıc 2021). If other wood materials are preferred according to the place of use, it may be recommended to use wood materials coated with 15% aloe vera added cellulosic varnish. Fig. 9. Yellow color tone (Δb*) variation for wood species - varnish and aloe vera mix type interaction 0 2 4 6 8 10 12 14 Control V & 5% Av V & 10% Av V & 15% Av 1 0 .4 3 9 .3 6 4 8 .8 2 1 8 .0 2 59 .9 8 9 9 .9 2 9 9 .4 9 7 8 .8 6 3 12.8 11.22 10.57 9.897 1 0 .8 2 8 .2 9 6 7 .2 6 .4 2 1 Y e ll o w c o lo r to n e ( Δ b *) Anatolian black pine Fir Ayous Scots pine PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6590 Using the differences of varnished and unvarnished measurements, were used to determine the interaction of aloe vera with cellulosic varnish. Total color change values were found different in terms of wood species and varnish and aloe vera mix type. Multi-factor analysis of variance was performed to determine the source of differences, and its results are shown in Table 11. Table 11. Variance Analysis for Changes in Total Color Tone (ΔE*) Source Degrees of Freedom Sum of Squares Mean Square F Value P ≤ 0.05 Wood Species (A) 3 206.290 68.763 40.6313 0.0000* Varnish and Aloe vera mix type (B) 3 349.436 116.479 68.8255 0.0000* AB 9 80.373 8.930 5.2768 0.0000* Error 176 297.858 1.692 Total 191 933.958 *: Difference is meaningful according to 0.05 According to the results of the variance analysis, wood species and varnish and aloe vera mix type and their mutual interactions were found statistically significant (P ≤ 0.05). Comparison of the results of Duncan test made at the level of wood species and varnish and aloe vera mix type are given in Table 12. Table 12. Comparison Result of Duncan Test Made at the Level of Wood Species and Varnish and Aloe Vera Mix Type Source x̄ HG Wood species Anatolian black pine 11.02 BC Fir 11.05 B Ayous 13.21 A* Scots pine 10.52 C Varnish and Aloe vera mix type Control 13.28 A Varnish and 5% Aloe vera (V and 5% Av) 11.89 B* Varnish and 10% Aloe vera (V and 10% Av) 11.07 C Varnish and 15% Aloe vera (V and 15% Av) 9.560 D LSD: ± 0.5237 *: The highest increase in total color change; x̄: Arithmetic mean; HG: Homogeneity group; Control: Varnish and 0% Aloe vera (V and 0% Av) According to Table 12, the highest change in total color tone was found in ayous wood materials and (varnish and 5% aloe vera) mix type, the lowest change in total color tone was found in Scots pine and (varnish and 15% aloe vera). Comparison results of the Duncan test made at the level of interaction between wood species and varnish and aloe vera mix type are given in Table 13, and the corresponding graph is shown in Fig. 10. PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6591 Table 13. Duncan Test Comparisons for Wood Species - Varnish and Aloe Vera Mix Type Interaction Wood Varnish Means HG Anatolian black pine Control 12.35 D Varnish and 5% Aloe vera 11.14 EF Varnish and 10% Aloe vera 10.79 FGH Varnish and 15% Aloe vera 9.818 H Fir Control 12.02 DE Varnish and 5% Aloe vera 11.10 EF Varnish and 10% Aloe vera 10.74 FGH Varnish and 15% Aloe vera 10.35 FGH Ayous Control 15.31 A Varnish and 5% Aloe vera 14.28 AB* Varnish and 10% Aloe vera 12.73 CD Varnish and 15% Aloe vera 10.51 FGH Scots pine Control 13.46 BC Varnish and 5% Aloe vera 11.05 EFG Varnish and 10% Aloe vera 10.01 GH Varnish and 15% Aloe vera 7.560 I** LSD: ± 1.047, *: The highest increase in total color change **: The lowest increase in total color change According to Table 13, the highest change in total color tone was seen in ayous over which varnish and 5% aloe vera was applied, whereas the lowest change was obtained in Scots pine over which varnish and 15% aloe vera was applied. It was determined that the total color change values of four tree species decreased as the percentage of aloe vera in the cellulosic varnish increased. It may be recommended to use Scots pine coated with 15% aloe vera added cellulosic varnish in applications where the total color tone does not change or changes little. It has been reported in literature that because ∆E* is in low value means color tone did not change or changed little. This situation is compatible with the literature (Sögütlü and Sönmez 2006). If other wood materials are preferred according to the place of use, it may be recommended to use wood materials coated with 15% aloe vera added cellulosic varnish. Fig. 10. Total color change (ΔE*) variation for wood species - varnish and aloe vera mix type interaction 0 2 4 6 8 10 12 14 16 Control V & 5% Av V & 10% Av V & 15% Av 1 2 .3 5 1 1 .1 4 1 0 .7 9 9 .8 1 81 2 .0 2 1 1 .1 1 0 .7 4 1 0 .3 5 15.31 14.28 12.73 10.51 1 3 .4 6 1 1 .0 5 1 0 .0 1 7 .5 6 T o ta l c o lo r c h a n g e ( Δ E *) Anatolian black pine Fir Ayous Scots pine PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6592 Glossiness perpendicular to fibers values obtained by the differences between varnished and unvarnished measurements were used to determine the interaction of aloe vera with cellulosic varnish. Glossiness perpendicular to fibers values were different in terms of wood species and varnish and aloe vera mix type. A multi-factor analysis of variance was done to determine the source of differences. Results are shown in Table 14. Table 14. Variance Analysis for Change in Glossiness Perpendicular to Fibers Source Degrees of Freedom Sum of Squares Mean Square F Value P ≤ 0.05 Wood Species (A) 3 5173.230 1724.410 46.7135 0.0000* Varnish and Aloe vera mix type (B) 3 20776.568 6925.523 187.6092 0.0000* AB 9 1744.588 193.843 5.2511 0.0000* Error 176 6496.973 36.915 Total 191 34191.359 *: Difference is meaningful according to 0.05 According to the results of the variance analysis, wood species and varnish and aloe vera mix type and their mutual interactions were found statistically significant (P ≤ 0.05). Comparison of the results of Duncan test made at the level of wood species and varnish and aloe vera mix type is given in Table 15. Table 15. Comparison Result of Duncan Test Made at the Level of Wood Species and Varnish and Aloe Vera Mix Type Source x̄ HG Wood species Anatolian black pine 72.869 A Fir 66.227 B Ayous 61.246 C Scots pine 74.063 A* Varnish and Aloe vera mix type Control 82.781 A Varnish and 5% Aloe vera (V and 5% Av) 72.879 B* Varnish and10% Aloe vera (V and 10% Av) 64.015 C Varnish and15% Aloe vera (V and 15% Av) 54.729 D LSD: ± 2.446 *: The highest increase in glossiness perpendicular to fibers; x̄: Arithmetic mean; HG: Homogeneity group; Control: Varnish and 0% Aloe vera (V and 0% Av) According to Table 15, the highest change in glossiness perpendicular to fibers was found in Scots pine wood materials and (varnish and 5% aloe vera) mix type, the lowest change in glossiness perpendicular to fibers was found in ayous and (varnish and 15% aloe vera). A comparison of the results of the Duncan test made at the level of interaction between wood species and varnish and aloe vera mix type is given in Table 16, and the corresponding graph is shown in Fig. 11. PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6593 Table 16. Duncan Test Comparisons for Wood Species - Varnish and Aloe Vera Mix Type Interaction Wood Varnish Means HG Anatolian black pine Control 82.542 A Varnish and 5% Aloe vera 78.808 A Varnish and10% Aloe vera 69.342 BC Varnish and15% Aloe vera 60.783 D Fir Control 83.600 A Varnish and 5% Aloe vera 66.875 C Varnish and 10% Aloe vera 61.350 D Varnish and 15% Aloe vera 53.083 E Ayous Control 81.383 A Varnish and 5% Aloe vera 66.533 C Varnish and 10% Aloe vera 52.717 E Varnish and 15% Aloe vera 44.350 F** Scots pine Control 83.600 A Varnish and 5% Aloe vera 79.300 A* Varnish and 10% Aloe vera 72.650 B Varnish and 15% Aloe vera 60.700 D LSD: ± 4.893, *: The highest increase in glossiness perpendicular to fibers **: The lowest increase in glossiness perpendicular to fibers According to Table 16, the highest change in glossiness perpendicular to fibers was seen in Scots pine over which varnish and 5% aloe vera was applied, whereas the lowest change was obtained in ayous over which varnish and 15% aloe vera was applied. It was determined that the glosssines values perpendicular to the fibers of four tree species decreased as the percentage of aloe vera in the cellulosic varnish increased. It may be recommended to use ayous coated with 15% aloe vera added cellulosic varnish in applications where the glossiness values does not change or changes little. If other wood materials are preferred according to the place of use, it may be recommended to use wood materials coated with 15% aloe vera added cellulosic varnish. Fig. 11. Glossiness perpendicular to fibers variation for wood species - varnish and aloe vera mix type interaction 0 10 20 30 40 50 60 70 80 90 Control V & 5% Av V & 10% Av V & 15% Av 8 2 .5 4 2 7 8 .8 0 8 6 9 .3 4 2 6 0 .7 8 3 8 3 .6 6 6 .8 7 5 6 1 .3 5 5 3 .0 8 3 8 1 .3 8 3 6 6 .5 3 3 5 2 .7 1 7 4 4 .3 5 8 3 .6 7 9 .3 7 2 .6 5 6 0 .7 G lo s s in e s s p e rp e n d ic u la r to fi b e rs Anatolian black pine Fir Ayous Scots pine PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6594 Glossiness parallel to fibers values, which were obtained by the differences varnished and unvarnished measurements, were used to determine the interaction of aloe vera with cellulosic varnish. Glossiness parallel to fibers values were found different in terms of wood species and varnish and aloe vera mix type. A multi-factor analysis of variance was performed to determine the source of differences, and its results are shown in Table 17. Table 17. Variance Analysis for Change in Glossiness Parallel to Fibers Source Degrees of Freedom Sum of Squares Mean Square F Value P ≤ 0.05 Wood Species (A) 3 4960.280 1653.427 58.6978 0.0000* Varnish and Aloe vera mix type (B) 3 14285.101 4761.700 169.0436 0.0000* AB 9 1849.157 205.462 7.2940 0.0000* Error 176 4957.652 28.168 Total 191 26052.189 *: Difference is meaningful according to 0.05 According to the results of the variance analysis, wood species and varnish and aloe vera mix type and their mutual interactions were found statistically significant (P ≤ 0.05). A comparison of the Duncan test results made at the level of wood species and varnish and aloe vera mix type is given in Table 18. Table 18. Duncan Test Made at the Level of Wood Species and Varnish and Aloe Vera Mix Type Source x̄ HG Wood species Anatolian black pine 91.546 A* Fir 83.996 B Ayous 79.363 C Scots pine 91.046 A Varnish and Aloe vera mix type Control 98.773 A Varnish and %5 Aloe vera (V and 5% Av) 89.540 B* Varnish and 10% Aloe vera (V and 10% Av) 81.960 C Varnish and 15% Aloe vera (V and 15% Av) 75.677 D LSD: ± 2.137 *: The highest increase in glossiness parallel to fibers x̄: Arithmetic mean; HG: Homogeneity group; Control: Varnish and 0% Aloe vera (V and 0% Av) According to Table 18, the highest change in glossiness parallel to fibers was found in Anatolian black pine wood materials and (varnish and 5% aloe vera) mix type, the lowest change in glossiness parallel to fibers was found in ayous and (varnish and 15% aloe vera). The comparison of Duncan test results made at the level of interaction between wood species and varnish and aloe vera mix type is given in Table 19, and the corresponding graph is shown in Fig. 12. PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6595 Table 19. Duncan Test Comparisons for Wood Species - Varnish and Aloe Vera Mix Type Interaction Wood Varnish Means HG Anatolian black pine Control 99.392 A Varnish and 5% Aloe vera 94.408 B* Varnish and 10% Aloe vera 89.033 CD Varnish and 15% Aloe vera 83.350 FG Fir Control 99.000 A Varnish and 5% Aloe vera 84.483 EF Varnish and 10% Aloe vera 79.367 G Varnish and 15% Aloe vera 73.133 H Ayous Control 97.042 AB Varnish and 5% Aloe vera 86.083 DEF Varnish and 10% Aloe vera 70.942 H Varnish and 15% Aloe vera 63.383 I** Scots pine Control 99.658 A Varnish and 5% Aloe vera 93.183 BC Varnish and 10% Aloe vera 88.500 DE Varnish and 15% Aloe vera 82.842 FG LSD: ± 4.274, *: The highest increase in glossiness parallel to fibers **: The lowest increase in glossiness parallel to fibers According to Table 19, the highest change in glossiness parallel to fibers was seen in Anatolian black pine over which varnish and 5% aloe vera was applied, whereas the lowest change was obtained in ayous over which varnish and 15% aloe vera was applied. It was determined that the glossiness values parallel to the fibers of four tree species decreased as the percentage of aloe vera in the cellulosic varnish increased. It may be recommended to use ayous coated with 15% aloe vera added cellulosic varnish in applications where the glossiness values does not change or changes little. If other wood materials are preferred according to the place of use, it may be recommended to use wood materials coated with 15% aloe vera added cellulosic varnish. Fig. 12. Glossiness parallel to fibers variation for wood species- varnish and aloe vera mix type interaction 0 10 20 30 40 50 60 70 80 90 100 Control V & 5% Av V & 10% Av V & 15% Av 9 9 .3 9 2 9 4 .4 0 8 8 9 .0 3 3 8 3 .3 5 9 9 8 4 .4 8 3 7 9 .3 6 7 7 3 .1 3 39 7 .0 4 2 8 6 .0 8 3 7 0 .9 4 2 6 3 .3 8 3 9 9 .6 5 8 9 3 .1 8 3 8 8 .5 8 2 .8 4 2 G lo s s in e s s p a ra ll e l to f ib e rs Anatolian black pine Fir Ayous Scots pine PEER-REVIEWED ARTICLE bioresources.cnr.ncsu.edu Yalinkilic (2025). “Aloe vera doped varnish for wood,” BioResources 20(3), 6577-6598. 6596 This study is original in that aloe vera is used for the first time in wood surface finishing. Further studies are needed to definitively determine the usability of aloe vera, which has a wide range of uses in other industries, also in the wood surface paint and varnish industry. It is recommended to conduct experiments such as hardness, bonding strength, surface roughness, wet temperature (hot water spillage), dry temperature (layer contact with hot food pot, etc.), resistance to cigarette burn, resistance to weathering and combustion test, etc. This study can be considered as a literature for future studies. CONCLUSIONS 1. There was an increase in the red color tone, yellow color tone, total color change, glossiness perpendicular to the fibers, and glossiness parallel to the fibers difference values in all of the control samples (due to varnish and 0% aloe vera). 2. It was determined that these difference values in all four tree species decreased as the percentage of aloe vera in cellulosic varnish increased. 3. It may be recommended to use 15% aloe vera added cellulosic varnish in applications where the color and the glossiness values does not change or changes little. 4. 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