BIOTROPIA Vol. 28 No. 1,2021: 74 - 83 DOI: 10.11598/btb.2021.28.1.1092 LAND SUITABILITY ASSESSMENT FOR PATCHOULI (Pogostemon cablin) DEVELOPMENT A N D ESSENTIAL OIL PRODUCTION AD1 SETIAWANl?'*, DEFFI ARMITA', ALDILA PUTlU RAHAYU' AND NUNUN BARUNAWATI' 'Department ofAgronomy, FaczrlQ ofAgni.nltzlre, Universio ofBrawzjqa, Jalan Veteran, Malang 65145, Indonesia 21nstzt~te ofAtxim', Universitas Brawzjqa, Jalan Veteran, Malang 65145, Indonesia Received 3 July 2018/Accepted 4 January 2020 Patchouli (Pogostemon cablin) is one of the important crop species in Indonesia, since over 80% of patchouli oil global market is produced in Indonesia. Pacthouli oil is the key ingredients for fragrance and aromatherapy products. Patchouli oil is extracted from the stems and leaves of pathouli plants. Therefore, it is important to improve Patchouli plant productivity and increase resources for sustainable patchouli cultivation. The suitability of abiotic factors in the growing environment of crops remarkably determines the success of crop production. This study aimed t o assess and evaluate land suitability for plant growth and development of Patchouli (Pogostemon cablin) in Dilem Wilis, Bendungan District, Trenggalek Regency, Indonesia. Initially, a survey was conducted and then an analysis was done to classify the land suitability for crops cultivation. The research was conducted on 3 locations from May t o July 2017 for land suitability and from July to November 2017 for Patchouli crops cultivation experiment. The results indicated that Location 1 had a land suitability of N class, implying that this location was not suitable due to its limiting factor of low I G O content (0.08 me/100 g). Meanwhile, both Location 2 and Location 3 showed similar land suitability class of S3s, tc, f, n signifying as less appropriate. The results of this study also indicated the influence of land suitability classes on plant growth however, the different classification (in this case S3 compared to N) did not demonstrate a correlation between land classes and oil yield and Patchouli alcohol, where the element Potassium was the limiting factor. Keywords: abiotic factor, land suitability, Patchouli alcohol, potassium INTRODUCTION Patchouli (Pogostemon cabhz) is one of the important crop species in Indonesia, since Indonesia is one of top producers of patchouli oil. Patchouli oil is used as key ingredients for fragrance as well as for cosmetics and aromatherapy products (Ramya et al. 2013; Nugraha et al. 2019). Indonesia is the world's largest producer of patchouli oil, accounting for 80% of the global market (I 3 3 Water availability (wa) Rainfalls (mm) 2300 - 3000 1750 - 2300 or 3000- 1200 - 1750 or > 5000 3500 3500 Root media (rc) Texture Drainage Soil depth (cm) Sandy clay, sandy Clay and other sandy clay, Clay quartz clay Other Other Very Good Good Bad Very Bad > 100 cm 75 - 100 cm 50 - 75 cm < 50 cm Nutrient retention (f) Acidity (pH) Acidic (5.5 - 7) Acidic to Neutral Acidic to Very Very Acidic to (5.5 - 5) Acidic (4,5-5) Alkaline (< 4.5 or > 7.5) C-Organic (YO) 2 - 3 3 - 5 < 2 CEC (me/100 g) > 17 5.6 < 5 Nutrition (n) I<2O (me/100 g) PZOS (ppm) Source: Djaenudin e t al. (2003). BIOTROPIA Vol. 28 No. 1,2021 The plant growth was assessed based on the branch height and canopy diameter of the plant at 2, 4 and 6 months after planting. Results were obtained by destructive sampling based on wetlfresh weight and curing dry weight (t- 20% water content). The oil yield component was obtained by the 8-hour steam distillation method to obtain Patchouli alcohol (PA) level as an indicator of the quahty of essential oils (Idris e t aL 2014; Bur6 & Selher 2004). The obtained 768 m asl. The average altitude for optimum Patchouli cultivation was at 780 m asl. Average air temperatures at each location based on the formula of Braak (1928) were: Location 1 at 21 OC, Location 2 at 21 OC, and Location 3 at 22 OC. The air temperature got cooler as the altitude went higher. Based on the formula of Braak (1928), the temperature registered a steady decline by 0.61 OC with an increasing altitude of 1 m. growth and yield data were tested by using The soil texture at the study site was analysis of variance (F-test) at significance level dominated by clay and dust. At Location 1, the P < 0.05). Furthermore, to test the significant soil texture was sandy clay, while at Location 2 differences between treatments, the Tukey test and Location 3 it was clay. Drainage at Location performance by R statistics was applied. 1 is considerably good as water moves quickly or seeps into the soil through the infiltration process, supported by sandy clay texture having RESULTS A N D DISCUSSION a larger pore than the sand fraction. Meanwhile, Land Suitability The differences among Location 1, Location 2 and Location 3, were based on the land suitabhty class (Location 1 is N as not appropriate, Locations 2 and 3 are less appropriate (Table 2). The slopes of Mount Wilis is considered hilly to mountainous reliefs. Location 1 has a slope of 8%, Location 2 has 10°/o, and Location 3 has 12%. At the observation point the altitude was about 600 - 850 m above sea level (m asl). Location 1 lies at an altitude of 792 m asl, Location 2 at an altitude of 809 m asl, and Location 3 at an altitude of both Location 2 and Location 3 have a good or medium drainage class. Soil acidity (pH) at Location 1 is 4.6, at Location 2 is 4.9 and at Location 3 is 4.5 (where the soil pH at the observation point is acidic). Based on laboratory test, C-Organic at the observation site was 1.63% at Location 1, 1.50% at Location 2 and 0.71% at Location 3. The Cation Exchange Capacity (CEC) at Location 1 is 18.75 me/100 g, at Location 2 is 5.38 me/100g, and at Location 3 is 18.45 me/100 g. The availability of I<20 macro elements at Location 1 is 0.08 me/100 g, at Location 2 is 0.46 me/100 g, and at Location 3 is 0.25 me/100 g. Table 2 Land suitabhty classification of location 1 , 2 and 3 at Dilem Wilis Plantation, Trenggalek Regency, Indonesia Land characteristics data Category Location 1 Location 2 Location 3 Land Position (s) Slope (O/o) 8% 10% 12% Altitude (m asl) 792 809 768 Temperature (tc) Average temperature ("C) 21 2 1 22 Water availability (wa) Rainfalls (mm) 1,428 1,428 1,428 Root Media (rc) Texture Clay Dust Clay Clay Drainage Good Good Very bad Soil ~ e p t h (cm) > 50 > 50 >-15 Nutrient retention (f) Acidity (pH) 4.6 4.9 4.5 C-Organic (O/o) 1.63 1.50 0.71 CEC (me/100 g) 18.75 5.38 18.45 Nutrition (n) IQO (me/ 1 00 g) 0.08 0.46 0.25 -. P205 (ppm) Land Suitability Class N n S3s, tc, f, n S3s, tc, f, n Source: Primary Data (2017) Assessing and evaluating land suitability in the development of patchouli effect on patchouli essential oil - Setiawan e t al. Crops Production Evaluation the number of branches and canopy diameter - . - did not differ sigmficantly. Correiation between Cmp Production Yield and ~ h , dfferent land classes produced higher Suitabikp biomass in plant fresh/wet weight and curing The results showed that the different land dry weight, indicating the effect of land classes significantly affected the plant height at 6 suitabiliq on plant growth (Fig. 6). months after planting (Figs. 3, 4, 5). However, Month after planting Figure 3 Number of branches Notes: a = 2 months; b = 4 months; c = 6 months after planting. Month after planting Figure 4 Plant height Notes: a = 2 months; b = 4 months; c = 6 months after planting; different letters in the figures indicate significant difference at 0.05 level. BIOTROPIA Vol. 28 No. 1,2021 Month after planting Figure 5 Canopy diameter among location (L) 1,2, and 3 on the 2, 4 and 6 months after planting Location Location Figure 6 Fresh weight (g per plant) Notes: a = curing dry weight (g per plant); b = harvested 6 months after planting; different letters in the figures indicate significant differences at 0.05 level. Although Location 1 has the lowest yield value, the average oil yield and Patchouli alcohol (PA) quality in each location were not significantly affected by the limiting factors (Table 3). Table 3 Average oil yield and quality PA (Patchouli Alcohol) of Patchouli Location Yield (?/o) PA (YO) Location 1 2.36 ns 23.40 ns Location 2 2.42 ns 22.82 ns Location 3 2.49 ns 24.14 ns Note: ns = no significant difference. have S3 class, tc, f, n indicating that the cultivation area was less suitable because of the limiting effect of the slope, altitude, temperature, pH, C-organic, and IGO. Potassium (I<) plays a key role in plant metabolism affecting the synthesis and accumulation of nutrients and secondary metabolites (Bihter e t a l 2016). Limiting factors that have significant effects included the altitude and the s l o ~ e s . The cultivation area was located on the southern slopes of Mount Wilis which is hilly to mountainous with an altitude of 600 - 850 m asl. The slope of the land varies. The land on slopes Patchouli Cultivation and Land Suitability were to erosion due to the lack of soil reinforcement plants and the absence of soil Matching the land characteristics with the protection from splashing rainwater. The land growth requirement of Patchouli resulted in on the slopes will be eroded if there is no Location 1 having a land suitability class "N", improvement on it. In the highlands with low indicating that the cultivated land was not temperatures, soil f e r a t y wdl be preserved, but suitable because of the limiting effect of the very steep slopes and unstable lands wdl lead to low IGO content. Location 2 and Location 3 landslides making it more suitable for trees Assessing and evaluating land suitability in the development ( ~f patchouli effect o n patchouli essential oil - Setiawan eta/ (Djaenudin 2008). The elevation of the observed location is in the range of > 700 m asl. Patchouli plants can grow and produce well at an altitude of 10 - 400 m above sea levels with air temperature ranging from 24 to 28 OC (Pujiharti et al. 2008). The other limiting factor was the acidic soil pH which has a value ranging from 4.5 to 4.9. The optimum growth and production of Patchouli plants requires optimum soil pH value of 5.5 to 7 which is quite acid to neutral. Nutrients and microorganisms are affected by the soil pH as nutrients are only available at certain pH level. The soil pH is influenced by the manner of land utiluation. The C-organic value, representing the organic matter percentage in the soil, at all the locations were also low (< 2%), as compared to the growth requirement of Patchouli plants whch is about 2 - 3%. I<20 content at all three locations was also low (< 0.6 me/100 g), lower than the optimal growth requirement of Patchouli (> 10 me/100 g of G O ) . Low I<20 content is due to several factors such as lack of fertihzation. Potassium is an alkaline cation, which balances charges of organic and inorganic anions activating more than 50 enzymes (Nurzynska- Wierdak et al. 201 1). Agronomic Factor Land suitability increased the plant growth despite the insignificantly different oil quantities (Figs. 3, 4, 5, 6; Table 3). The current study showed that the effect of limiting factors (potassium) and land suitability does not affect Patchouli oil and alcohol yields, similar results with Singh (201 4). However, distinguishable differences were observed in the potassium content among location 1, 2 and 3 (Table 2). Potassium is considered a plant essential mineral which has h g h concentration in the meristematic tissues and in the phloem. However, I< uptake by the plant roots is accomplished by at least two distinct hnetic systems such as the h g h and low affinity I<+ transporters (Hafsi et al. 2014). Potassium serves as an important element in plant metabolism, promoting carbohydrates, fats and protein synthesis, increasing crop yield and improving fresh produce quality. Moreover, I< enables plants efficacy to resist pests and diseases as well as acting as enzymes co-factor, including enzymes related to the essential oil synthesis (Hafsi et al. 2014). The application of I< has also affected the growth and essential oil yield of lemongrass (Cymbopogon flxaoszls), dittany (Origdnum dictamnas), basil (Ocimm basilicam) and rosemary (Rosmrinus ofiknalis) (Economakis 1993; Puttanna et al. 2010). Improvement of Land Characteristics and Cultivation The improvement of land characteristic was aimed at optimizing the land condition for Patchouli cultivation by using the limiting factors as a reference. The slope of the land is unchangeable, yet such condition could still be addressed by constructing terraces to reduce surface runoff that leads to erosion (Wkunan et al. 1985); and the position of the porch slope affects the reception of light (Auslander et al. 2003). Fertilization is another alternative to improve the soil pH value, I<20 content, and c- organic percentage, including appropriate fertilization system such as: fertilization time and method, and fertilizer type selection. The application of organic fertilizer is intended to supply the nutrients that could not be provided by the chemical fertilizer, and also to improve the physical and biological properties of the soil (Abdurachman e t al. 2008). Liming is another method applied to increase soil acidity values (pH) to be more neutral during land cultivation. Potassium (I<) affects the growth and essential oil synthesis in aromatic plants as it is required by plants to build abundant organic compounds such as amino acids, proteins, enzymes and nucleic acids. These minerals affect the function and levels of enzymes involved in the terpenoides biosynthesis (Hafsi et al. 2014). The low potassium content at Location 1 is a h t i n g factor. However, certain strategies could be applied to ensure the quantity of essential oil yields, where the main target of the harvest is secondary metabolites. It is necessary to synchronize the suitabihty of the growth conditions for secondary metabolite-producing plants in as much as both the growth and yield targets also implied the achievement of good quality essential oils. The planting patterns are also considered as a solution to increase stress as the harvesting period approaches (Sacks et al. 2010). BIOTROPIA Vol. 28 No. 1,2021 CONCLUSION The land suitability analysis of the three sampling locations (Location 1, 2, 3) revealed that Location 1 is not suitable for the growing of Patchouli because of its low IG0 content at 0.08 me/100 g. Both Location 2 and Location 3 showed similar land suitability class of S3s, tc, f, which is less appropriate, because of the limiting effects of land, altitude, rainfall, air temperature, C-organic, soil pH (acidity), and I