id	author	title	date	pages	extension	mime	words	sentence	flesch	summary	cache	txt
biotropia-329	Stephanie, Stephanie; Widyastuti, Utut; Wiyono, Suryo	INTRODUCTION OF THE SERINE GREEN FLUORESCENT PROTEIN (sGFP) GENE INTO Pyricularia grisea RACE DC4 ISOLATED FROM Digitaria ciliaris USING Agrobacterium tumefaciens-MEDIATED GENETIC TRANSFORMATION	2015	7	.pdf	application/pdf	3737	227	60	M: Marker 1 kb; K+ dan : plasmid pCAMB-P.grisea sGFP sGFP-Nos sGFP -Tubulin P. grisea P. grisea , K+ : ras 173 (positive control); K-: ddH2O (negative control); 1: DNA dc4 β nontransformant; 2-3: DNA dc4 transformant P. grisea sGFP Figure . The isolated DNA sGFP gene as then w amplified through PCR using -F and -R primers as well as sGFP sGFP combined primers of F and Nos-R. The sGFP- PCR mixture used consisted of 1 µL (100 ng) genomic DNA, 0.5 mM forward primers, 0.5 mM reverse primers, 5 µL PCR mix (Fermentas) and added with ddH O up to 10 L of total volume.	cache/biotropia-329.pdf	txt/biotropia-329.txt