id	sid	tid	token	lemma	pos
biotropia-134	1	1	2	2	NUM
biotropia-134	1	2	development	development	NOUN
biotropia-134	1	3	og	og	PROPN
biotropia-134	1	4	gene.cdr	gene.cdr	PROPN
biotropia-134	1	5	biotropia	biotropia	NOUN
biotropia-134	1	6	vol	vol	NOUN
biotropia-134	1	7	.	.	PROPN
biotropia-134	1	8	18	18	NUM
biotropia-134	1	9	no	no	NOUN
biotropia-134	1	10	.	.	NOUN
biotropia-134	1	11	1	1	NUM
biotropia-134	1	12	,	,	PUNCT
biotropia-134	1	13	2011	2011	NUM
biotropia-134	1	14	:	:	PUNCT
biotropia-134	1	15	13	13	NUM
biotropia-134	1	16	23	23	NUM
biotropia-134	1	17	13	13	NUM
biotropia-134	1	18	comparison	comparison	NOUN
biotropia-134	1	19	of	of	ADP
biotropia-134	1	20	three	three	NUM
biotropia-134	1	21	different	different	ADJ
biotropia-134	1	22	techniques	technique	NOUN
biotropia-134	1	23	of	of	ADP
biotropia-134	1	24	gene	gene	NOUN
biotropia-134	1	25	transfer	transfer	NOUN
biotropia-134	1	26	in	in	ADP
biotropia-134	1	27	humpback	humpback	NOUN
biotropia-134	1	28	grouper	grouper	NOUN
biotropia-134	1	29	(	(	PUNCT
biotropia-134	1	30	)	)	PUNCT
biotropia-134	1	31	cromileptes	cromilepte	VERB
biotropia-134	1	32	altivelis	altiveli	VERB
biotropia-134	1	33	slamet	slamet	PROPN
biotropia-134	1	34	subyakto	subyakto	NOUN
biotropia-134	1	35	,	,	PUNCT
biotropia-134	1	36	alimuddin	alimuddin	PROPN
biotropia-134	1	37	,	,	PUNCT
biotropia-134	1	38	rustidja	rustidja	PROPN
biotropia-134	1	39	,	,	PUNCT
biotropia-134	1	40	m.	m.	PROPN
biotropia-134	1	41	sasmito	sasmito	PROPN
biotropia-134	1	42	jati	jati	PROPN
biotropia-134	1	43	,	,	PUNCT
biotropia-134	1	44	irvan	irvan	PROPN
biotropia-134	1	45	faizal	faizal	PROPN
biotropia-134	1	46	,	,	PUNCT
biotropia-134	1	47	ratu	ratu	PROPN
biotropia-134	1	48	siti	siti	PROPN
biotropia-134	1	49	aliah	aliah	PROPN
biotropia-134	1	50	,	,	PUNCT
biotropia-134	1	51	gemi	gemi	PROPN
biotropia-134	1	52	triastutik	triastutik	PROPN
biotropia-134	1	53	,	,	PUNCT
biotropia-134	1	54	and	and	CCONJ
biotropia-134	1	55	komar	komar	PROPN
biotropia-134	1	56	sumantadinata	sumantadinata	PROPN
biotropia-134	1	57	humpback	humpback	NOUN
biotropia-134	1	58	grouper	grouper	NOUN
biotropia-134	1	59	is	be	AUX
biotropia-134	1	60	one	one	NUM
biotropia-134	1	61	of	of	ADP
biotropia-134	1	62	the	the	DET
biotropia-134	1	63	most	most	ADV
biotropia-134	1	64	cultured	cultured	ADJ
biotropia-134	1	65	fishes	fish	NOUN
biotropia-134	1	66	in	in	ADP
biotropia-134	1	67	asia	asia	PROPN
biotropia-134	1	68	,	,	PUNCT
biotropia-134	1	69	including	include	VERB
biotropia-134	1	70	indonesia	indonesia	PROPN
biotropia-134	1	71	.	.	PUNCT
biotropia-134	2	1	the	the	DET
biotropia-134	2	2	main	main	ADJ
biotropia-134	2	3	problem	problem	NOUN
biotropia-134	2	4	faced	face	VERB
biotropia-134	2	5	by	by	ADP
biotropia-134	2	6	humpback	humpback	NOUN
biotropia-134	2	7	culture	culture	NOUN
biotropia-134	2	8	is	be	AUX
biotropia-134	2	9	its	its	PRON
biotropia-134	2	10	slow	slow	ADJ
biotropia-134	2	11	growth	growth	NOUN
biotropia-134	2	12	rate	rate	NOUN
biotropia-134	2	13	.	.	PUNCT
biotropia-134	3	1	one	one	NUM
biotropia-134	3	2	of	of	ADP
biotropia-134	3	3	the	the	DET
biotropia-134	3	4	methods	method	NOUN
biotropia-134	3	5	that	that	PRON
biotropia-134	3	6	will	will	AUX
biotropia-134	3	7	be	be	AUX
biotropia-134	3	8	more	more	ADV
biotropia-134	3	9	effective	effective	ADJ
biotropia-134	3	10	and	and	CCONJ
biotropia-134	3	11	efficient	efficient	ADJ
biotropia-134	3	12	to	to	PART
biotropia-134	3	13	solve	solve	VERB
biotropia-134	3	14	the	the	DET
biotropia-134	3	15	problem	problem	NOUN
biotropia-134	3	16	is	be	AUX
biotropia-134	3	17	using	use	VERB
biotropia-134	3	18	transgenic	transgenic	NOUN
biotropia-134	3	19	technique	technique	NOUN
biotropia-134	3	20	.	.	PUNCT
biotropia-134	4	1	this	this	DET
biotropia-134	4	2	study	study	NOUN
biotropia-134	4	3	was	be	AUX
biotropia-134	4	4	conducted	conduct	VERB
biotropia-134	4	5	to	to	PART
biotropia-134	4	6	determine	determine	VERB
biotropia-134	4	7	the	the	DET
biotropia-134	4	8	effectiveness	effectiveness	NOUN
biotropia-134	4	9	of	of	ADP
biotropia-134	4	10	transfection	transfection	NOUN
biotropia-134	4	11	,	,	PUNCT
biotropia-134	4	12	microinjection	microinjection	NOUN
biotropia-134	4	13	and	and	CCONJ
biotropia-134	4	14	electroporation	electroporation	NOUN
biotropia-134	4	15	techniques	technique	NOUN
biotropia-134	4	16	on	on	ADP
biotropia-134	4	17	gene	gene	NOUN
biotropia-134	4	18	transfer	transfer	NOUN
biotropia-134	4	19	in	in	ADP
biotropia-134	4	20	humpback	humpback	NOUN
biotropia-134	4	21	grouper	grouper	NOUN
biotropia-134	4	22	.	.	PUNCT
biotropia-134	5	1	transfection	transfection	NOUN
biotropia-134	5	2	was	be	AUX
biotropia-134	5	3	performed	perform	VERB
biotropia-134	5	4	by	by	ADP
biotropia-134	5	5	incubating	incubate	VERB
biotropia-134	5	6	sperm	sperm	NOUN
biotropia-134	5	7	to	to	ADP
biotropia-134	5	8	the	the	DET
biotropia-134	5	9	foreign	foreign	ADJ
biotropia-134	5	10	dna	dna	NOUN
biotropia-134	5	11	(	(	PUNCT
biotropia-134	5	12	pktbp	pktbp	NOUN
biotropia-134	5	13	-	-	PUNCT
biotropia-134	5	14	ktgh	ktgh	NOUN
biotropia-134	5	15	gene	gene	NOUN
biotropia-134	5	16	construct)-transfectant	construct)-transfectant	PROPN
biotropia-134	5	17	complex	complex	ADJ
biotropia-134	5	18	solution	solution	NOUN
biotropia-134	5	19	,	,	PUNCT
biotropia-134	5	20	while	while	SCONJ
biotropia-134	5	21	was	be	AUX
biotropia-134	5	22	by	by	ADP
biotropia-134	5	23	injecting	inject	VERB
biotropia-134	5	24	those	those	DET
biotropia-134	5	25	complex	complex	ADJ
biotropia-134	5	26	solution	solution	NOUN
biotropia-134	5	27	into	into	ADP
biotropia-134	5	28	testis	testis	NOUN
biotropia-134	5	29	of	of	ADP
biotropia-134	5	30	mature	mature	ADJ
biotropia-134	5	31	males	male	NOUN
biotropia-134	5	32	.	.	PUNCT
biotropia-134	6	1	microinjection	microinjection	NOUN
biotropia-134	6	2	was	be	AUX
biotropia-134	6	3	conducted	conduct	VERB
biotropia-134	6	4	in	in	ADP
biotropia-134	6	5	2	2	NUM
biotropia-134	6	6	-	-	SYM
biotropia-134	6	7	4	4	NUM
biotropia-134	6	8	cell	cell	NOUN
biotropia-134	6	9	stage	stage	NOUN
biotropia-134	6	10	embryos	embryo	NOUN
biotropia-134	6	11	using	use	VERB
biotropia-134	6	12	25	25	NUM
biotropia-134	6	13	μg	μg	PROPN
biotropia-134	6	14	/	/	SYM
biotropia-134	6	15	ml	ml	PROPN
biotropia-134	6	16	of	of	ADP
biotropia-134	6	17	foreign	foreign	ADJ
biotropia-134	6	18	dna	dna	NOUN
biotropia-134	6	19	solution	solution	NOUN
biotropia-134	6	20	,	,	PUNCT
biotropia-134	6	21	and	and	CCONJ
biotropia-134	6	22	duration	duration	NOUN
biotropia-134	6	23	of	of	ADP
biotropia-134	6	24	injection	injection	NOUN
biotropia-134	6	25	was	be	AUX
biotropia-134	6	26	1	1	NUM
biotropia-134	6	27	,	,	PUNCT
biotropia-134	6	28	2	2	NUM
biotropia-134	6	29	and	and	CCONJ
biotropia-134	6	30	3	3	NUM
biotropia-134	6	31	seconds	second	NOUN
biotropia-134	6	32	.	.	PUNCT
biotropia-134	7	1	electroporation	electroporation	NOUN
biotropia-134	7	2	by	by	ADP
biotropia-134	7	3	50	50	NUM
biotropia-134	7	4	v	v	NOUN
biotropia-134	7	5	,	,	PUNCT
biotropia-134	7	6	30	30	NUM
biotropia-134	7	7	ms	ms	NOUN
biotropia-134	7	8	of	of	ADP
biotropia-134	7	9	pulse	pulse	NOUN
biotropia-134	7	10	length	length	NOUN
biotropia-134	7	11	,	,	PUNCT
biotropia-134	7	12	5	5	NUM
biotropia-134	7	13	of	of	ADP
biotropia-134	7	14	pulse	pulse	NOUN
biotropia-134	7	15	number	number	NOUN
biotropia-134	7	16	and	and	CCONJ
biotropia-134	7	17	0.1	0.1	NUM
biotropia-134	7	18	of	of	ADP
biotropia-134	7	19	pulse	pulse	NOUN
biotropia-134	7	20	interval	interval	NOUN
biotropia-134	7	21	was	be	AUX
biotropia-134	7	22	performed	perform	VERB
biotropia-134	7	23	to	to	ADP
biotropia-134	7	24	sperm	sperm	NOUN
biotropia-134	7	25	using	use	VERB
biotropia-134	7	26	three	three	NUM
biotropia-134	7	27	dna	dna	ADJ
biotropia-134	7	28	concentration	concentration	NOUN
biotropia-134	7	29	of	of	ADP
biotropia-134	7	30	5	5	NUM
biotropia-134	7	31	,	,	PUNCT
biotropia-134	7	32	10	10	NUM
biotropia-134	7	33	and	and	CCONJ
biotropia-134	7	34	20	20	NUM
biotropia-134	7	35	μg	μg	NOUN
biotropia-134	7	36	/	/	SYM
biotropia-134	7	37	ml	ml	PROPN
biotropia-134	7	38	.	.	PUNCT
biotropia-134	8	1	the	the	DET
biotropia-134	8	2	incorporation	incorporation	NOUN
biotropia-134	8	3	of	of	ADP
biotropia-134	8	4	foreign	foreign	ADJ
biotropia-134	8	5	dna	dna	NOUN
biotropia-134	8	6	in	in	ADP
biotropia-134	8	7	sperm	sperm	NOUN
biotropia-134	8	8	and	and	CCONJ
biotropia-134	8	9	embryos	embryo	NOUN
biotropia-134	8	10	were	be	AUX
biotropia-134	8	11	analyzed	analyze	VERB
biotropia-134	8	12	using	use	VERB
biotropia-134	8	13	pcr	pcr	ADJ
biotropia-134	8	14	method	method	NOUN
biotropia-134	8	15	.	.	PUNCT
biotropia-134	9	1	based	base	VERB
biotropia-134	9	2	on	on	ADP
biotropia-134	9	3	pcr	pcr	ADJ
biotropia-134	9	4	analysis	analysis	NOUN
biotropia-134	9	5	,	,	PUNCT
biotropia-134	9	6	an	an	DET
biotropia-134	9	7	optimum	optimum	ADJ
biotropia-134	9	8	dna	dna	NOUN
biotropia-134	9	9	concentration	concentration	NOUN
biotropia-134	9	10	for	for	ADP
biotropia-134	9	11	electroporation	electroporation	NOUN
biotropia-134	9	12	was	be	AUX
biotropia-134	9	13	10	10	NUM
biotropia-134	9	14	μg	μg	NOUN
biotropia-134	9	15	/	/	SYM
biotropia-134	9	16	ml	ml	PROPN
biotropia-134	9	17	.	.	PUNCT
biotropia-134	9	18	limited	limited	ADJ
biotropia-134	9	19	number	number	NOUN
biotropia-134	9	20	of	of	ADP
biotropia-134	9	21	embryos	embryo	NOUN
biotropia-134	9	22	could	could	AUX
biotropia-134	9	23	be	be	AUX
biotropia-134	9	24	microinjected	microinjecte	VERB
biotropia-134	9	25	during	during	ADP
biotropia-134	9	26	20	20	NUM
biotropia-134	9	27	-	-	SYM
biotropia-134	9	28	30	30	NUM
biotropia-134	9	29	min	min	NOUN
biotropia-134	9	30	to	to	PART
biotropia-134	9	31	reach	reach	VERB
biotropia-134	9	32	2	2	NUM
biotropia-134	9	33	-	-	SYM
biotropia-134	9	34	4	4	NUM
biotropia-134	9	35	cell	cell	NOUN
biotropia-134	9	36	stage	stage	NOUN
biotropia-134	9	37	.	.	PUNCT
biotropia-134	10	1	microinjection	microinjection	NOUN
biotropia-134	10	2	for	for	ADP
biotropia-134	10	3	1	1	NUM
biotropia-134	10	4	second	second	NOUN
biotropia-134	10	5	showed	show	VERB
biotropia-134	10	6	higher	high	ADJ
biotropia-134	10	7	survival	survival	NOUN
biotropia-134	10	8	rate	rate	NOUN
biotropia-134	10	9	of	of	ADP
biotropia-134	10	10	embryos	embryo	NOUN
biotropia-134	10	11	,	,	PUNCT
biotropia-134	10	12	although	although	SCONJ
biotropia-134	10	13	none	none	NOUN
biotropia-134	10	14	or	or	CCONJ
biotropia-134	10	15	very	very	ADV
biotropia-134	10	16	low	low	ADJ
biotropia-134	10	17	number	number	NOUN
biotropia-134	10	18	of	of	ADP
biotropia-134	10	19	larvae	larvae	NOUN
biotropia-134	10	20	was	be	AUX
biotropia-134	10	21	hatched	hatch	VERB
biotropia-134	10	22	.	.	PUNCT
biotropia-134	11	1	transfast	transfast	NOUN
biotropia-134	11	2	was	be	AUX
biotropia-134	11	3	an	an	DET
biotropia-134	11	4	effective	effective	ADJ
biotropia-134	11	5	dna	dna	NOUN
biotropia-134	11	6	delivery	delivery	NOUN
biotropia-134	11	7	reagent	reagent	NOUN
biotropia-134	11	8	for	for	ADP
biotropia-134	11	9	humpback	humpback	NOUN
biotropia-134	11	10	grouper	grouper	NOUN
biotropia-134	11	11	sperm	sperm	NOUN
biotropia-134	11	12	.	.	PUNCT
biotropia-134	12	1	foreign	foreign	ADJ
biotropia-134	12	2	dna	dna	PROPN
biotropia-134	12	3	could	could	AUX
biotropia-134	12	4	be	be	AUX
biotropia-134	12	5	detected	detect	VERB
biotropia-134	12	6	in	in	ADP
biotropia-134	12	7	sperm	sperm	NOUN
biotropia-134	12	8	from	from	ADP
biotropia-134	12	9	two	two	NUM
biotropia-134	12	10	out	out	ADP
biotropia-134	12	11	of	of	ADP
biotropia-134	12	12	ten	ten	NUM
biotropia-134	12	13	transfected	transfecte	VERB
biotropia-134	12	14	fish	fish	NOUN
biotropia-134	12	15	at	at	ADP
biotropia-134	12	16	least	least	ADV
biotropia-134	12	17	36	36	NUM
biotropia-134	12	18	hours	hour	NOUN
biotropia-134	12	19	post	post	NOUN
biotropia-134	12	20	transfection	transfection	NOUN
biotropia-134	12	21	(	(	PUNCT
biotropia-134	12	22	hpt	hpt	NOUN
biotropia-134	12	23	)	)	PUNCT
biotropia-134	12	24	.	.	PUNCT
biotropia-134	13	1	by	by	ADP
biotropia-134	13	2	transfection	transfection	NOUN
biotropia-134	13	3	,	,	PUNCT
biotropia-134	13	4	foreign	foreign	ADJ
biotropia-134	13	5	dna	dna	NOUN
biotropia-134	13	6	was	be	AUX
biotropia-134	13	7	detected	detect	VERB
biotropia-134	13	8	in	in	ADP
biotropia-134	13	9	sperm	sperm	NOUN
biotropia-134	13	10	at	at	ADP
biotropia-134	13	11	48	48	NUM
biotropia-134	13	12	hpt	hpt	NOUN
biotropia-134	13	13	25	25	NUM
biotropia-134	13	14	c	c	NOUN
biotropia-134	13	15	incubation	incubation	NOUN
biotropia-134	13	16	temperature	temperature	NOUN
biotropia-134	13	17	.	.	PUNCT
biotropia-134	14	1	our	our	PRON
biotropia-134	14	2	study	study	NOUN
biotropia-134	14	3	revealed	reveal	VERB
biotropia-134	14	4	that	that	SCONJ
biotropia-134	14	5	transfection	transfection	NOUN
biotropia-134	14	6	,	,	PUNCT
biotropia-134	14	7	microinjection	microinjection	NOUN
biotropia-134	14	8	as	as	ADV
biotropia-134	14	9	well	well	ADV
biotropia-134	14	10	as	as	ADP
biotropia-134	14	11	electroporation	electroporation	NOUN
biotropia-134	14	12	could	could	AUX
biotropia-134	14	13	be	be	AUX
biotropia-134	14	14	used	use	VERB
biotropia-134	14	15	as	as	ADP
biotropia-134	14	16	transgenesis	transgenesis	NOUN
biotropia-134	14	17	methods	method	NOUN
biotropia-134	14	18	in	in	ADP
biotropia-134	14	19	humpback	humpback	NOUN
biotropia-134	14	20	grouper	grouper	NOUN
biotropia-134	14	21	.	.	PUNCT
biotropia-134	15	1	by	by	ADP
biotropia-134	15	2	means	mean	NOUN
biotropia-134	15	3	of	of	ADP
biotropia-134	15	4	simplicity	simplicity	NOUN
biotropia-134	15	5	and	and	CCONJ
biotropia-134	15	6	efficacy	efficacy	NOUN
biotropia-134	15	7	,	,	PUNCT
biotropia-134	15	8	however	however	ADV
biotropia-134	15	9	,	,	PUNCT
biotropia-134	15	10	electroporation	electroporation	NOUN
biotropia-134	15	11	was	be	AUX
biotropia-134	15	12	an	an	DET
biotropia-134	15	13	appropriate	appropriate	ADJ
biotropia-134	15	14	gene	gene	NOUN
biotropia-134	15	15	transfer	transfer	NOUN
biotropia-134	15	16	method	method	NOUN
biotropia-134	15	17	.	.	PUNCT
biotropia-134	16	1	transfection	transfection	NOUN
biotropia-134	16	2	,	,	PUNCT
biotropia-134	16	3	microinjection	microinjection	NOUN
biotropia-134	16	4	,	,	PUNCT
biotropia-134	16	5	electroporation	electroporation	NOUN
biotropia-134	16	6	,	,	PUNCT
biotropia-134	16	7	transgenesis	transgenesis	NOUN
biotropia-134	16	8	,	,	PUNCT
biotropia-134	16	9	humpback	humpback	NOUN
biotropia-134	16	10	grouper	grouper	NOUN
biotropia-134	16	11	1	1	NUM
biotropia-134	16	12	2	2	NUM
biotropia-134	16	13	3	3	NUM
biotropia-134	16	14	4	4	NUM
biotropia-134	16	15	5	5	NUM
biotropia-134	16	16	5	5	NUM
biotropia-134	16	17	1	1	NUM
biotropia-134	16	18	2	2	NUM
biotropia-134	16	19	o	o	NOUN
biotropia-134	16	20	*	*	NOUN
biotropia-134	16	21	1	1	NUM
biotropia-134	16	22	2	2	NUM
biotropia-134	16	23	3	3	NUM
biotropia-134	16	24	4	4	NUM
biotropia-134	16	25	5	5	NUM
biotropia-134	16	26	brackishwater	brackishwater	NOUN
biotropia-134	16	27	aquaculture	aquaculture	NOUN
biotropia-134	16	28	development	development	NOUN
biotropia-134	16	29	center	center	NOUN
biotropia-134	16	30	,	,	PUNCT
biotropia-134	16	31	situbondo	situbondo	PROPN
biotropia-134	16	32	,	,	PUNCT
biotropia-134	16	33	ministry	ministry	PROPN
biotropia-134	16	34	of	of	ADP
biotropia-134	16	35	marine	marine	PROPN
biotropia-134	16	36	affairs	affairs	PROPN
biotropia-134	16	37	and	and	CCONJ
biotropia-134	16	38	fisheries	fishery	NOUN
biotropia-134	16	39	,	,	PUNCT
biotropia-134	16	40	indonesia	indonesia	PROPN
biotropia-134	16	41	department	department	PROPN
biotropia-134	16	42	of	of	ADP
biotropia-134	16	43	aquaculture	aquaculture	NOUN
biotropia-134	16	44	,	,	PUNCT
biotropia-134	16	45	faculty	faculty	NOUN
biotropia-134	16	46	of	of	ADP
biotropia-134	16	47	fisheries	fishery	NOUN
biotropia-134	16	48	and	and	CCONJ
biotropia-134	16	49	marine	marine	ADJ
biotropia-134	16	50	science	science	NOUN
biotropia-134	16	51	,	,	PUNCT
biotropia-134	16	52	bogor	bogor	X
biotropia-134	16	53	agricultural	agricultural	ADJ
biotropia-134	16	54	university	university	PROPN
biotropia-134	16	55	,	,	PUNCT
biotropia-134	16	56	indonesia	indonesia	PROPN
biotropia-134	16	57	faculty	faculty	NOUN
biotropia-134	16	58	of	of	ADP
biotropia-134	16	59	fisheries	fishery	NOUN
biotropia-134	16	60	and	and	CCONJ
biotropia-134	16	61	marine	marine	ADJ
biotropia-134	16	62	science	science	NOUN
biotropia-134	16	63	,	,	PUNCT
biotropia-134	16	64	university	university	NOUN
biotropia-134	16	65	of	of	ADP
biotropia-134	16	66	brawijaya	brawijaya	PROPN
biotropia-134	16	67	,	,	PUNCT
biotropia-134	16	68	malang	malang	PROPN
biotropia-134	16	69	,	,	PUNCT
biotropia-134	16	70	indonesia	indonesia	PROPN
biotropia-134	16	71	faculty	faculty	NOUN
biotropia-134	16	72	of	of	ADP
biotropia-134	16	73	mathematics	mathematic	NOUN
biotropia-134	16	74	and	and	CCONJ
biotropia-134	16	75	natural	natural	ADJ
biotropia-134	16	76	sciences	science	NOUN
biotropia-134	16	77	,	,	PUNCT
biotropia-134	16	78	university	university	NOUN
biotropia-134	16	79	of	of	ADP
biotropia-134	16	80	brawijaya	brawijaya	PROPN
biotropia-134	16	81	,	,	PUNCT
biotropia-134	16	82	malang	malang	PROPN
biotropia-134	16	83	,	,	PUNCT
biotropia-134	16	84	indonesia	indonesia	PROPN
biotropia-134	16	85	agency	agency	PROPN
biotropia-134	16	86	for	for	ADP
biotropia-134	16	87	the	the	DET
biotropia-134	16	88	assessment	assessment	NOUN
biotropia-134	16	89	and	and	CCONJ
biotropia-134	16	90	application	application	NOUN
biotropia-134	16	91	of	of	ADP
biotropia-134	16	92	technology	technology	NOUN
biotropia-134	16	93	,	,	PUNCT
biotropia-134	16	94	jakarta	jakarta	PROPN
biotropia-134	16	95	,	,	PUNCT
biotropia-134	16	96	indonesia	indonesia	PROPN
biotropia-134	16	97	in	in	ADP
biotropia-134	16	98	vitro	vitro	X
biotropia-134	16	99	in	in	ADP
biotropia-134	16	100	vivo	vivo	NOUN
biotropia-134	16	101	in	in	ADP
biotropia-134	16	102	vivo	vivo	NOUN
biotropia-134	16	103	in	in	ADP
biotropia-134	16	104	vitro	vitro	X
biotropia-134	16	105	abstract	abstract	ADJ
biotropia-134	16	106	key	key	ADJ
biotropia-134	16	107	words	word	NOUN
biotropia-134	16	108	:	:	PUNCT
biotropia-134	16	109	*	*	PUNCT
biotropia-134	16	110	corresponding	correspond	VERB
biotropia-134	16	111	author	author	NOUN
biotropia-134	16	112	:	:	PUNCT
biotropia-134	16	113	alimuddin_alsani@yahoo.com	alimuddin_alsani@yahoo.com	X
biotropia-134	16	114	biotropia	biotropia	PROPN
biotropia-134	16	115	vol	vol	NOUN
biotropia-134	16	116	.	.	PROPN
biotropia-134	17	1	18	18	NUM
biotropia-134	17	2	no	no	NOUN
biotropia-134	17	3	.	.	NOUN
biotropia-134	17	4	1	1	NUM
biotropia-134	17	5	,	,	PUNCT
biotropia-134	17	6	2011	2011	NUM
biotropia-134	17	7	14	14	NUM
biotropia-134	17	8	introduction	introduction	NOUN
biotropia-134	17	9	materials	material	NOUN
biotropia-134	17	10	and	and	CCONJ
biotropia-134	17	11	method	method	NOUN
biotropia-134	17	12	in	in	ADP
biotropia-134	17	13	the	the	DET
biotropia-134	17	14	last	last	ADJ
biotropia-134	17	15	two	two	NUM
biotropia-134	17	16	decades	decade	NOUN
biotropia-134	17	17	,	,	PUNCT
biotropia-134	17	18	a	a	DET
biotropia-134	17	19	variety	variety	NOUN
biotropia-134	17	20	of	of	ADP
biotropia-134	17	21	transgenic	transgenic	NOUN
biotropia-134	17	22	aquatic	aquatic	ADJ
biotropia-134	17	23	organisms	organism	NOUN
biotropia-134	17	24	have	have	AUX
biotropia-134	17	25	been	be	AUX
biotropia-134	17	26	successfully	successfully	ADV
biotropia-134	17	27	produced	produce	VERB
biotropia-134	17	28	by	by	ADP
biotropia-134	17	29	researchers	researcher	NOUN
biotropia-134	17	30	around	around	ADP
biotropia-134	17	31	the	the	DET
biotropia-134	17	32	world	world	NOUN
biotropia-134	17	33	.	.	PUNCT
biotropia-134	18	1	these	these	DET
biotropia-134	18	2	transgenic	transgenic	NOUN
biotropia-134	18	3	organisms	organism	NOUN
biotropia-134	18	4	have	have	AUX
biotropia-134	18	5	been	be	AUX
biotropia-134	18	6	produced	produce	VERB
biotropia-134	18	7	by	by	ADP
biotropia-134	18	8	introducing	introduce	VERB
biotropia-134	18	9	gene	gene	NOUN
biotropia-134	18	10	/	/	SYM
biotropia-134	18	11	transgene	transgene	NOUN
biotropia-134	18	12	encoding	encode	VERB
biotropia-134	18	13	protein	protein	NOUN
biotropia-134	18	14	using	use	VERB
biotropia-134	18	15	various	various	ADJ
biotropia-134	18	16	transgenesis	transgenesis	NOUN
biotropia-134	18	17	methods	method	NOUN
biotropia-134	18	18	,	,	PUNCT
biotropia-134	18	19	that	that	PRON
biotropia-134	18	20	is	be	AUX
biotropia-134	18	21	microinjection	microinjection	NOUN
biotropia-134	18	22	,	,	PUNCT
biotropia-134	18	23	electroporation	electroporation	NOUN
biotropia-134	18	24	,	,	PUNCT
biotropia-134	18	25	transfection	transfection	NOUN
biotropia-134	18	26	and	and	CCONJ
biotropia-134	18	27	particle	particle	NOUN
biotropia-134	18	28	gun	gun	NOUN
biotropia-134	18	29	bombardment	bombardment	NOUN
biotropia-134	18	30	(	(	PUNCT
biotropia-134	18	31	chen	chen	PROPN
biotropia-134	18	32	.	.	PUNCT
biotropia-134	19	1	1998	1998	NUM
biotropia-134	19	2	;	;	PUNCT
biotropia-134	19	3	sarmasik	sarmasik	PROPN
biotropia-134	19	4	2001	2001	NUM
biotropia-134	19	5	)	)	PUNCT
biotropia-134	19	6	.	.	PUNCT
biotropia-134	20	1	transgene	transgene	NOUN
biotropia-134	20	2	can	can	AUX
biotropia-134	20	3	be	be	AUX
biotropia-134	20	4	integrated	integrate	VERB
biotropia-134	20	5	and	and	CCONJ
biotropia-134	20	6	expressed	express	VERB
biotropia-134	20	7	in	in	ADP
biotropia-134	20	8	descendants	descendant	NOUN
biotropia-134	20	9	of	of	ADP
biotropia-134	20	10	transgenic	transgenic	NOUN
biotropia-134	20	11	fish	fish	NOUN
biotropia-134	20	12	.	.	PUNCT
biotropia-134	21	1	furthermore	furthermore	ADV
biotropia-134	21	2	,	,	PUNCT
biotropia-134	21	3	transgenic	transgenic	NOUN
biotropia-134	21	4	organisms	organism	NOUN
biotropia-134	21	5	have	have	AUX
biotropia-134	21	6	produced	produce	VERB
biotropia-134	21	7	the	the	DET
biotropia-134	21	8	expected	expect	VERB
biotropia-134	21	9	phenotypes	phenotype	NOUN
biotropia-134	21	10	.	.	PUNCT
biotropia-134	22	1	until	until	ADP
biotropia-134	22	2	now	now	ADV
biotropia-134	22	3	,	,	PUNCT
biotropia-134	22	4	most	most	ADJ
biotropia-134	22	5	of	of	ADP
biotropia-134	22	6	the	the	DET
biotropia-134	22	7	generated	generate	VERB
biotropia-134	22	8	transgenic	transgenic	NOUN
biotropia-134	22	9	aquatic	aquatic	ADJ
biotropia-134	22	10	organisms	organism	NOUN
biotropia-134	22	11	are	be	AUX
biotropia-134	22	12	freshwater	freshwater	ADJ
biotropia-134	22	13	fish	fish	NOUN
biotropia-134	22	14	because	because	SCONJ
biotropia-134	22	15	these	these	DET
biotropia-134	22	16	fish	fish	NOUN
biotropia-134	22	17	are	be	AUX
biotropia-134	22	18	relatively	relatively	ADV
biotropia-134	22	19	easy	easy	ADJ
biotropia-134	22	20	to	to	PART
biotropia-134	22	21	maintain	maintain	VERB
biotropia-134	22	22	and	and	CCONJ
biotropia-134	22	23	spawn	spawn	VERB
biotropia-134	22	24	under	under	ADP
biotropia-134	22	25	laboratory	laboratory	NOUN
biotropia-134	22	26	condition	condition	NOUN
biotropia-134	22	27	.	.	PUNCT
biotropia-134	23	1	in	in	ADP
biotropia-134	23	2	contrast	contrast	NOUN
biotropia-134	23	3	,	,	PUNCT
biotropia-134	23	4	marine	marine	ADJ
biotropia-134	23	5	fish	fish	NOUN
biotropia-134	23	6	receive	receive	VERB
biotropia-134	23	7	very	very	ADV
biotropia-134	23	8	little	little	ADJ
biotropia-134	23	9	attention	attention	NOUN
biotropia-134	23	10	despite	despite	SCONJ
biotropia-134	23	11	the	the	DET
biotropia-134	23	12	fact	fact	NOUN
biotropia-134	23	13	that	that	SCONJ
biotropia-134	23	14	marine	marine	ADJ
biotropia-134	23	15	fish	fish	NOUN
biotropia-134	23	16	have	have	AUX
biotropia-134	23	17	a	a	DET
biotropia-134	23	18	high	high	ADJ
biotropia-134	23	19	potential	potential	ADJ
biotropia-134	23	20	economic	economic	ADJ
biotropia-134	23	21	value	value	NOUN
biotropia-134	23	22	in	in	ADP
biotropia-134	23	23	aquaculture	aquaculture	NOUN
biotropia-134	23	24	.	.	PUNCT
biotropia-134	24	1	humpback	humpback	NOUN
biotropia-134	24	2	grouper	grouper	NOUN
biotropia-134	24	3	is	be	AUX
biotropia-134	24	4	one	one	NUM
biotropia-134	24	5	of	of	ADP
biotropia-134	24	6	the	the	DET
biotropia-134	24	7	most	most	ADV
biotropia-134	24	8	cultured	cultured	ADJ
biotropia-134	24	9	fishes	fish	NOUN
biotropia-134	24	10	in	in	ADP
biotropia-134	24	11	asia	asia	PROPN
biotropia-134	24	12	,	,	PUNCT
biotropia-134	24	13	including	include	VERB
biotropia-134	24	14	indonesia	indonesia	PROPN
biotropia-134	24	15	.	.	PUNCT
biotropia-134	25	1	the	the	DET
biotropia-134	25	2	main	main	ADJ
biotropia-134	25	3	problem	problem	NOUN
biotropia-134	25	4	faced	face	VERB
biotropia-134	25	5	in	in	ADP
biotropia-134	25	6	humpback	humpback	NOUN
biotropia-134	25	7	culture	culture	NOUN
biotropia-134	25	8	is	be	AUX
biotropia-134	25	9	its	its	PRON
biotropia-134	25	10	slow	slow	ADJ
biotropia-134	25	11	growth	growth	NOUN
biotropia-134	25	12	rate	rate	NOUN
biotropia-134	25	13	.	.	PUNCT
biotropia-134	26	1	for	for	ADP
biotropia-134	26	2	instance	instance	NOUN
biotropia-134	26	3	,	,	PUNCT
biotropia-134	26	4	to	to	PART
biotropia-134	26	5	reach	reach	VERB
biotropia-134	26	6	consumption	consumption	NOUN
biotropia-134	26	7	size	size	NOUN
biotropia-134	26	8	of	of	ADP
biotropia-134	26	9	about	about	ADV
biotropia-134	26	10	0.5	0.5	NUM
biotropia-134	26	11	kg	kg	PROPN
biotropia-134	26	12	1.0	1.0	NUM
biotropia-134	26	13	kg	kg	NOUN
biotropia-134	26	14	,	,	PUNCT
biotropia-134	26	15	this	this	DET
biotropia-134	26	16	species	specie	NOUN
biotropia-134	26	17	needs	need	VERB
biotropia-134	26	18	8	8	NUM
biotropia-134	26	19	up	up	ADP
biotropia-134	26	20	to	to	PART
biotropia-134	26	21	24	24	NUM
biotropia-134	26	22	months	month	NOUN
biotropia-134	26	23	(	(	PUNCT
biotropia-134	26	24	tucker	tucker	PROPN
biotropia-134	26	25	1991	1991	NUM
biotropia-134	26	26	;	;	PUNCT
biotropia-134	26	27	teitelbaum	teitelbaum	NOUN
biotropia-134	26	28	2007	2007	NUM
biotropia-134	26	29	)	)	PUNCT
biotropia-134	26	30	.	.	PUNCT
biotropia-134	27	1	its	its	PRON
biotropia-134	27	2	slow	slow	ADJ
biotropia-134	27	3	growth	growth	NOUN
biotropia-134	27	4	makes	make	VERB
biotropia-134	27	5	the	the	DET
biotropia-134	27	6	operational	operational	ADJ
biotropia-134	27	7	cost	cost	NOUN
biotropia-134	27	8	of	of	ADP
biotropia-134	27	9	this	this	DET
biotropia-134	27	10	fish	fish	NOUN
biotropia-134	27	11	aquaculture	aquaculture	NOUN
biotropia-134	27	12	increases	increase	VERB
biotropia-134	27	13	drastically	drastically	ADV
biotropia-134	27	14	.	.	PUNCT
biotropia-134	28	1	therefore	therefore	ADV
biotropia-134	28	2	,	,	PUNCT
biotropia-134	28	3	developing	develop	VERB
biotropia-134	28	4	a	a	DET
biotropia-134	28	5	proper	proper	ADJ
biotropia-134	28	6	method	method	NOUN
biotropia-134	28	7	that	that	PRON
biotropia-134	28	8	will	will	AUX
biotropia-134	28	9	be	be	AUX
biotropia-134	28	10	more	more	ADV
biotropia-134	28	11	effective	effective	ADJ
biotropia-134	28	12	and	and	CCONJ
biotropia-134	28	13	efficient	efficient	ADJ
biotropia-134	28	14	to	to	PART
biotropia-134	28	15	solve	solve	VERB
biotropia-134	28	16	the	the	DET
biotropia-134	28	17	problem	problem	NOUN
biotropia-134	28	18	is	be	AUX
biotropia-134	28	19	urgently	urgently	ADV
biotropia-134	28	20	needed	need	VERB
biotropia-134	28	21	.	.	PUNCT
biotropia-134	29	1	application	application	NOUN
biotropia-134	29	2	of	of	ADP
biotropia-134	29	3	transgenesis	transgenesis	NOUN
biotropia-134	29	4	technique	technique	NOUN
biotropia-134	29	5	is	be	AUX
biotropia-134	29	6	expected	expect	VERB
biotropia-134	29	7	to	to	PART
biotropia-134	29	8	solve	solve	VERB
biotropia-134	29	9	the	the	DET
biotropia-134	29	10	problem	problem	NOUN
biotropia-134	29	11	for	for	ADP
biotropia-134	29	12	humpback	humpback	NOUN
biotropia-134	29	13	grouper	grouper	NOUN
biotropia-134	29	14	and	and	CCONJ
biotropia-134	29	15	also	also	ADV
biotropia-134	29	16	other	other	ADJ
biotropia-134	29	17	marine	marine	ADJ
biotropia-134	29	18	fish	fish	NOUN
biotropia-134	29	19	,	,	PUNCT
biotropia-134	29	20	though	though	SCONJ
biotropia-134	29	21	the	the	DET
biotropia-134	29	22	use	use	NOUN
biotropia-134	29	23	of	of	ADP
biotropia-134	29	24	transgenic	transgenic	NOUN
biotropia-134	29	25	organisms	organism	NOUN
biotropia-134	29	26	in	in	ADP
biotropia-134	29	27	aquaculture	aquaculture	NOUN
biotropia-134	29	28	remains	remain	VERB
biotropia-134	29	29	controversial	controversial	ADJ
biotropia-134	29	30	.	.	PUNCT
biotropia-134	30	1	several	several	ADJ
biotropia-134	30	2	methods	method	NOUN
biotropia-134	30	3	of	of	ADP
biotropia-134	30	4	transgenesis	transgenesis	NOUN
biotropia-134	30	5	have	have	AUX
biotropia-134	30	6	been	be	AUX
biotropia-134	30	7	successfully	successfully	ADV
biotropia-134	30	8	applied	apply	VERB
biotropia-134	30	9	to	to	PART
biotropia-134	30	10	create	create	VERB
biotropia-134	30	11	transgenic	transgenic	NOUN
biotropia-134	30	12	fish	fish	NOUN
biotropia-134	30	13	.	.	PUNCT
biotropia-134	31	1	devlin	devlin	NOUN
biotropia-134	31	2	(	(	PUNCT
biotropia-134	31	3	1994	1994	NUM
biotropia-134	31	4	)	)	PUNCT
biotropia-134	31	5	utilized	utilize	VERB
biotropia-134	31	6	microinjection	microinjection	NOUN
biotropia-134	31	7	method	method	NOUN
biotropia-134	31	8	to	to	PART
biotropia-134	31	9	generate	generate	VERB
biotropia-134	31	10	transgenic	transgenic	NOUN
biotropia-134	31	11	fish	fish	NOUN
biotropia-134	31	12	.	.	PUNCT
biotropia-134	32	1	pacific	pacific	ADJ
biotropia-134	32	2	salmon	salmon	PROPN
biotropia-134	32	3	,	,	PUNCT
biotropia-134	32	4	alimuddin	alimuddin	PROPN
biotropia-134	32	5	(	(	PUNCT
biotropia-134	32	6	2005	2005	NUM
biotropia-134	32	7	)	)	PUNCT
biotropia-134	32	8	succeeded	succeed	VERB
biotropia-134	32	9	in	in	ADP
biotropia-134	32	10	zebrafish	zebrafish	NUM
biotropia-134	32	11	.	.	PUNCT
biotropia-134	33	1	electroporation	electroporation	NOUN
biotropia-134	33	2	method	method	NOUN
biotropia-134	33	3	has	have	AUX
biotropia-134	33	4	also	also	ADV
biotropia-134	33	5	been	be	AUX
biotropia-134	33	6	successfully	successfully	ADV
biotropia-134	33	7	used	use	VERB
biotropia-134	33	8	by	by	ADP
biotropia-134	33	9	sin	sin	NOUN
biotropia-134	33	10	.	.	PUNCT
biotropia-134	34	1	(	(	PUNCT
biotropia-134	34	2	1993	1993	NUM
biotropia-134	34	3	)	)	PUNCT
biotropia-134	34	4	and	and	CCONJ
biotropia-134	34	5	symons	symon	NOUN
biotropia-134	34	6	.	.	PUNCT
biotropia-134	35	1	(	(	PUNCT
biotropia-134	35	2	1994	1994	NUM
biotropia-134	35	3	)	)	PUNCT
biotropia-134	35	4	on	on	ADP
biotropia-134	35	5	production	production	NOUN
biotropia-134	35	6	of	of	ADP
biotropia-134	35	7	transgenic	transgenic	NOUN
biotropia-134	35	8	salmon	salmon	NOUN
biotropia-134	35	9	,	,	PUNCT
biotropia-134	35	10	while	while	SCONJ
biotropia-134	35	11	patil	patil	PROPN
biotropia-134	35	12	and	and	CCONJ
biotropia-134	35	13	khoo	khoo	PROPN
biotropia-134	35	14	(	(	PUNCT
biotropia-134	35	15	1996	1996	NUM
biotropia-134	35	16	)	)	PUNCT
biotropia-134	35	17	and	and	CCONJ
biotropia-134	35	18	rambubu	rambubu	VERB
biotropia-134	35	19	(	(	PUNCT
biotropia-134	35	20	2005	2005	NUM
biotropia-134	35	21	)	)	PUNCT
biotropia-134	35	22	succeeded	succeed	VERB
biotropia-134	35	23	in	in	ADP
biotropia-134	35	24	zebrafish	zebrafish	ADJ
biotropia-134	35	25	.	.	PUNCT
biotropia-134	36	1	the	the	DET
biotropia-134	36	2	transfection	transfection	NOUN
biotropia-134	36	3	method	method	NOUN
biotropia-134	36	4	was	be	AUX
biotropia-134	36	5	successfully	successfully	ADV
biotropia-134	36	6	applied	apply	VERB
biotropia-134	36	7	for	for	ADP
biotropia-134	36	8	gene	gene	NOUN
biotropia-134	36	9	transfer	transfer	NOUN
biotropia-134	36	10	in	in	ADP
biotropia-134	36	11	silver	silver	ADJ
biotropia-134	36	12	snapper	snapper	NOUN
biotropia-134	36	13	(	(	PUNCT
biotropia-134	36	14	lu	lu	NOUN
biotropia-134	36	15	2002	2002	NUM
biotropia-134	36	16	)	)	PUNCT
biotropia-134	36	17	and	and	CCONJ
biotropia-134	36	18	white	white	PROPN
biotropia-134	36	19	shrimp	shrimp	PROPN
biotropia-134	36	20	(	(	PUNCT
biotropia-134	36	21	sun	sun	NOUN
biotropia-134	36	22	2005	2005	NUM
biotropia-134	36	23	)	)	PUNCT
biotropia-134	36	24	.	.	PUNCT
biotropia-134	37	1	this	this	DET
biotropia-134	37	2	study	study	NOUN
biotropia-134	37	3	was	be	AUX
biotropia-134	37	4	conducted	conduct	VERB
biotropia-134	37	5	to	to	PART
biotropia-134	37	6	determine	determine	VERB
biotropia-134	37	7	the	the	DET
biotropia-134	37	8	effectiveness	effectiveness	NOUN
biotropia-134	37	9	of	of	ADP
biotropia-134	37	10	transfection	transfection	NOUN
biotropia-134	37	11	,	,	PUNCT
biotropia-134	37	12	microinjection	microinjection	NOUN
biotropia-134	37	13	and	and	CCONJ
biotropia-134	37	14	electroporation	electroporation	NOUN
biotropia-134	37	15	methods	method	NOUN
biotropia-134	37	16	in	in	ADP
biotropia-134	37	17	gene	gene	NOUN
biotropia-134	37	18	transfer	transfer	NOUN
biotropia-134	37	19	for	for	ADP
biotropia-134	37	20	humpback	humpback	NOUN
biotropia-134	37	21	grouper	grouper	NOUN
biotropia-134	37	22	.	.	PUNCT
biotropia-134	38	1	transfection	transfection	NOUN
biotropia-134	38	2	method	method	NOUN
biotropia-134	38	3	is	be	AUX
biotropia-134	38	4	one	one	NUM
biotropia-134	38	5	of	of	ADP
biotropia-134	38	6	gene	gene	NOUN
biotropia-134	38	7	transfer	transfer	NOUN
biotropia-134	38	8	methods	method	NOUN
biotropia-134	38	9	using	use	VERB
biotropia-134	38	10	a	a	DET
biotropia-134	38	11	specific	specific	ADJ
biotropia-134	38	12	reagent	reagent	NOUN
biotropia-134	38	13	that	that	PRON
biotropia-134	38	14	is	be	AUX
biotropia-134	38	15	capable	capable	ADJ
biotropia-134	38	16	of	of	ADP
biotropia-134	38	17	binding	bind	VERB
biotropia-134	38	18	to	to	ADP
biotropia-134	38	19	and	and	CCONJ
biotropia-134	38	20	delivering	deliver	VERB
biotropia-134	38	21	foreign	foreign	ADJ
biotropia-134	38	22	dna	dna	NOUN
biotropia-134	38	23	to	to	PART
biotropia-134	38	24	enter	enter	VERB
biotropia-134	38	25	the	the	DET
biotropia-134	38	26	cell	cell	NOUN
biotropia-134	38	27	.	.	PUNCT
biotropia-134	39	1	foreign	foreign	ADJ
biotropia-134	39	2	dna	dna	PROPN
biotropia-134	39	3	used	use	VERB
biotropia-134	39	4	in	in	ADP
biotropia-134	39	5	this	this	DET
biotropia-134	39	6	study	study	NOUN
biotropia-134	39	7	was	be	AUX
biotropia-134	39	8	all	all	ADV
biotropia-134	39	9	-	-	PUNCT
biotropia-134	39	10	humpback	humpback	NOUN
biotropia-134	39	11	grouper	grouper	NOUN
biotropia-134	39	12	gene	gene	NOUN
biotropia-134	39	13	construct	construct	NOUN
biotropia-134	39	14	,	,	PUNCT
biotropia-134	39	15	pktbp	pktbp	NOUN
biotropia-134	39	16	-	-	PUNCT
biotropia-134	39	17	ktgh	ktgh	NOUN
biotropia-134	39	18	,	,	PUNCT
biotropia-134	39	19	which	which	PRON
biotropia-134	39	20	has	have	AUX
biotropia-134	39	21	been	be	AUX
biotropia-134	39	22	constructed	construct	VERB
biotropia-134	39	23	by	by	ADP
biotropia-134	39	24	rusnas	rusnas	PROPN
biotropia-134	39	25	(	(	PUNCT
biotropia-134	39	26	national	national	ADJ
biotropia-134	39	27	strategic	strategic	ADJ
biotropia-134	39	28	prime	prime	ADJ
biotropia-134	39	29	research	research	NOUN
biotropia-134	39	30	)	)	PUNCT
biotropia-134	39	31	program	program	NOUN
biotropia-134	39	32	of	of	ADP
biotropia-134	39	33	ministry	ministry	PROPN
biotropia-134	39	34	of	of	ADP
biotropia-134	39	35	research	research	NOUN
biotropia-134	39	36	and	and	CCONJ
biotropia-134	39	37	technology	technology	NOUN
biotropia-134	39	38	,	,	PUNCT
biotropia-134	39	39	republic	republic	NOUN
biotropia-134	39	40	indonesia	indonesia	PROPN
biotropia-134	39	41	)	)	PUNCT
biotropia-134	39	42	for	for	ADP
biotropia-134	39	43	genetic	genetic	ADJ
biotropia-134	39	44	improvement	improvement	NOUN
biotropia-134	39	45	of	of	ADP
biotropia-134	39	46	hump	hump	NOUN
biotropia-134	39	47	et	et	PROPN
biotropia-134	39	48	al	al	PROPN
biotropia-134	39	49	et	et	PROPN
biotropia-134	39	50	al	al	PROPN
biotropia-134	39	51	.	.	PROPN
biotropia-134	39	52	et	et	PROPN
biotropia-134	40	1	al	al	PROPN
biotropia-134	40	2	.	.	PROPN
biotropia-134	40	3	et	et	PROPN
biotropia-134	40	4	al	al	PROPN
biotropia-134	40	5	.	.	PROPN
biotropia-134	40	6	et	et	PROPN
biotropia-134	40	7	al	al	PROPN
biotropia-134	40	8	et	et	PROPN
biotropia-134	40	9	al	al	PROPN
biotropia-134	40	10	et	et	PROPN
biotropia-134	40	11	al	al	PROPN
biotropia-134	40	12	.	.	PROPN
biotropia-134	40	13	et	et	PROPN
biotropia-134	41	1	al	al	PROPN
biotropia-134	41	2	.	.	PROPN
biotropia-134	41	3	et	et	PROPN
biotropia-134	42	1	al	al	PROPN
biotropia-134	42	2	.	.	PROPN
biotropia-134	42	3	transfection	transfection	PROPN
biotropia-134	42	4	back	back	PROPN
biotropia-134	42	5	grouper	grouper	NOUN
biotropia-134	42	6	research	research	NOUN
biotropia-134	42	7	team	team	NOUN
biotropia-134	42	8	in	in	ADP
biotropia-134	42	9	2007	2007	NUM
biotropia-134	42	10	.	.	PUNCT
biotropia-134	43	1	pktbp	pktbp	NOUN
biotropia-134	43	2	-	-	PUNCT
biotropia-134	43	3	ktgh	ktgh	NOUN
biotropia-134	43	4	contains	contain	VERB
biotropia-134	43	5	growth	growth	NOUN
biotropia-134	43	6	hormone	hormone	NOUN
biotropia-134	43	7	gene	gene	NOUN
biotropia-134	43	8	(	(	PUNCT
biotropia-134	43	9	ktgh	ktgh	PROPN
biotropia-134	43	10	)	)	PUNCT
biotropia-134	43	11	and	and	CCONJ
biotropia-134	43	12	β	β	NOUN
biotropia-134	43	13	-	-	ADJ
biotropia-134	43	14	actin	actin	ADJ
biotropia-134	43	15	promoter	promoter	NOUN
biotropia-134	43	16	(	(	PUNCT
biotropia-134	43	17	ktbp	ktbp	PROPN
biotropia-134	43	18	)	)	PUNCT
biotropia-134	43	19	from	from	ADP
biotropia-134	43	20	humpback	humpback	NOUN
biotropia-134	43	21	grouper	grouper	NOUN
biotropia-134	43	22	.	.	PUNCT
biotropia-134	44	1	15	15	NUM
biotropia-134	44	2	gene	gene	NOUN
biotropia-134	44	3	transfer	transfer	NOUN
biotropia-134	44	4	method	method	NOUN
biotropia-134	44	5	in	in	ADP
biotropia-134	44	6	humpback	humpback	NOUN
biotropia-134	44	7	grouper	grouper	NOUN
biotropia-134	44	8	alimuddin	alimuddin	PROPN
biotropia-134	44	9	.et	.et	PUNCT
biotropia-134	45	1	al	al	PROPN
biotropia-134	45	2	a.	a.	PROPN
biotropia-134	45	3	transfection	transfection	PROPN
biotropia-134	45	4	b.	b.	PROPN
biotropia-134	45	5	transfection	transfection	PROPN
biotropia-134	45	6	microinjection	microinjection	PROPN
biotropia-134	45	7	electroporation	electroporation	VERB
biotropia-134	45	8	dna	dna	NOUN
biotropia-134	45	9	extraction	extraction	NOUN
biotropia-134	45	10	preliminary	preliminary	ADJ
biotropia-134	45	11	experiment	experiment	NOUN
biotropia-134	45	12	was	be	AUX
biotropia-134	45	13	conducted	conduct	VERB
biotropia-134	45	14	by	by	ADP
biotropia-134	45	15	two	two	NUM
biotropia-134	45	16	transfectanst	transfectanst	PROPN
biotropia-134	45	17	,	,	PUNCT
biotropia-134	45	18	i.e.	i.e.	X
biotropia-134	45	19	transfast	transfast	NOUN
biotropia-134	45	20	(	(	PUNCT
biotropia-134	45	21	promega	promega	NOUN
biotropia-134	45	22	)	)	PUNCT
biotropia-134	45	23	and	and	CCONJ
biotropia-134	45	24	jetpei	jetpei	PROPN
biotropia-134	45	25	(	(	PUNCT
biotropia-134	45	26	polyplus	polyplus	ADJ
biotropia-134	45	27	)	)	PUNCT
biotropia-134	45	28	to	to	PART
biotropia-134	45	29	determine	determine	VERB
biotropia-134	45	30	their	their	PRON
biotropia-134	45	31	efficacy	efficacy	NOUN
biotropia-134	45	32	to	to	PART
biotropia-134	45	33	deliver	deliver	VERB
biotropia-134	45	34	pktbp	pktbp	NOUN
biotropia-134	45	35	-	-	PUNCT
biotropia-134	45	36	ktgh	ktgh	NOUN
biotropia-134	45	37	into	into	ADP
biotropia-134	45	38	sperm	sperm	NOUN
biotropia-134	45	39	.	.	PUNCT
biotropia-134	46	1	in	in	ADP
biotropia-134	46	2	this	this	DET
biotropia-134	46	3	experiment	experiment	NOUN
biotropia-134	46	4	,	,	PUNCT
biotropia-134	46	5	100	100	NUM
biotropia-134	46	6	μl	μl	ADP
biotropia-134	46	7	of	of	ADP
biotropia-134	46	8	grouper	grouper	NOUN
biotropia-134	46	9	sperm	sperm	NOUN
biotropia-134	46	10	was	be	AUX
biotropia-134	46	11	exposed	expose	VERB
biotropia-134	46	12	to	to	ADP
biotropia-134	46	13	360	360	NUM
biotropia-134	46	14	μl	μl	ADP
biotropia-134	46	15	of	of	ADP
biotropia-134	46	16	physiological	physiological	ADJ
biotropia-134	46	17	solution	solution	NOUN
biotropia-134	46	18	containing	contain	VERB
biotropia-134	46	19	10	10	NUM
biotropia-134	46	20	μl	μl	ADP
biotropia-134	46	21	pktbp	pktbp	NOUN
biotropia-134	46	22	-	-	PUNCT
biotropia-134	46	23	ktgh	ktgh	NOUN
biotropia-134	46	24	and	and	CCONJ
biotropia-134	46	25	either	either	DET
biotropia-134	46	26	30	30	NUM
biotropia-134	46	27	μl	μl	ADP
biotropia-134	46	28	transfast	transfast	NOUN
biotropia-134	46	29	or	or	CCONJ
biotropia-134	46	30	jetpei	jetpei	NOUN
biotropia-134	46	31	,	,	PUNCT
biotropia-134	46	32	at	at	ADP
biotropia-134	46	33	17ºc	17ºc	ADJ
biotropia-134	46	34	and	and	CCONJ
biotropia-134	46	35	25ºc	25ºc	ADJ
biotropia-134	46	36	incubation	incubation	NOUN
biotropia-134	46	37	temperature	temperature	NOUN
biotropia-134	46	38	for	for	ADP
biotropia-134	46	39	24	24	NUM
biotropia-134	46	40	,	,	PUNCT
biotropia-134	46	41	48	48	NUM
biotropia-134	46	42	,	,	PUNCT
biotropia-134	46	43	and	and	CCONJ
biotropia-134	46	44	72	72	NUM
biotropia-134	46	45	hours	hour	NOUN
biotropia-134	46	46	transfection	transfection	NOUN
biotropia-134	46	47	.	.	PUNCT
biotropia-134	47	1	plasmid	plasmid	ADJ
biotropia-134	47	2	pktbp	pktbp	NOUN
biotropia-134	47	3	-	-	PUNCT
biotropia-134	47	4	ktgh	ktgh	NOUN
biotropia-134	47	5	was	be	AUX
biotropia-134	47	6	injected	inject	VERB
biotropia-134	47	7	into	into	ADP
biotropia-134	47	8	humpback	humpback	NOUN
biotropia-134	47	9	grouper	grouper	NOUN
biotropia-134	47	10	testes	testis	NOUN
biotropia-134	47	11	prior	prior	ADV
biotropia-134	47	12	to	to	ADP
biotropia-134	47	13	spawning	spawning	NOUN
biotropia-134	47	14	according	accord	VERB
biotropia-134	47	15	to	to	ADP
biotropia-134	47	16	the	the	DET
biotropia-134	47	17	method	method	NOUN
biotropia-134	47	18	of	of	ADP
biotropia-134	47	19	lu	lu	PROPN
biotropia-134	47	20	.	.	PUNCT
biotropia-134	48	1	(	(	PUNCT
biotropia-134	48	2	2002	2002	NUM
biotropia-134	48	3	)	)	PUNCT
biotropia-134	48	4	with	with	ADP
biotropia-134	48	5	slight	slight	ADJ
biotropia-134	48	6	modifications	modification	NOUN
biotropia-134	48	7	.	.	PUNCT
biotropia-134	49	1	foreign	foreign	ADJ
biotropia-134	49	2	dna	dna	NOUN
biotropia-134	49	3	-	-	PUNCT
biotropia-134	49	4	transfast	transfast	NOUN
biotropia-134	49	5	complex	complex	ADJ
biotropia-134	49	6	solution	solution	NOUN
biotropia-134	49	7	at	at	ADP
biotropia-134	49	8	the	the	DET
biotropia-134	49	9	solution	solution	NOUN
biotropia-134	49	10	was	be	AUX
biotropia-134	49	11	injected	inject	VERB
biotropia-134	49	12	into	into	ADP
biotropia-134	49	13	the	the	DET
biotropia-134	49	14	right	right	NOUN
biotropia-134	49	15	and	and	CCONJ
biotropia-134	49	16	left	leave	VERB
biotropia-134	49	17	testes	testis	NOUN
biotropia-134	49	18	of	of	ADP
biotropia-134	49	19	mature	mature	ADJ
biotropia-134	49	20	broodstock	broodstock	NOUN
biotropia-134	49	21	,	,	PUNCT
biotropia-134	49	22	respectively	respectively	ADV
biotropia-134	49	23	.	.	PUNCT
biotropia-134	50	1	at	at	ADP
biotropia-134	50	2	12	12	NUM
biotropia-134	50	3	,	,	PUNCT
biotropia-134	50	4	36	36	NUM
biotropia-134	50	5	,	,	PUNCT
biotropia-134	50	6	48	48	NUM
biotropia-134	50	7	,	,	PUNCT
biotropia-134	50	8	60	60	NUM
biotropia-134	50	9	and	and	CCONJ
biotropia-134	50	10	72	72	NUM
biotropia-134	50	11	hours	hour	NOUN
biotropia-134	50	12	post	post	NOUN
biotropia-134	50	13	injection	injection	NOUN
biotropia-134	50	14	,	,	PUNCT
biotropia-134	50	15	dna	dna	NOUN
biotropia-134	50	16	from	from	ADP
biotropia-134	50	17	the	the	DET
biotropia-134	50	18	transfected	transfecte	VERB
biotropia-134	50	19	sperm	sperm	NOUN
biotropia-134	50	20	was	be	AUX
biotropia-134	50	21	extracted	extract	VERB
biotropia-134	50	22	and	and	CCONJ
biotropia-134	50	23	used	use	VERB
biotropia-134	50	24	as	as	ADP
biotropia-134	50	25	template	template	NOUN
biotropia-134	50	26	of	of	ADP
biotropia-134	50	27	pcr	pcr	ADJ
biotropia-134	50	28	amplification	amplification	NOUN
biotropia-134	50	29	to	to	PART
biotropia-134	50	30	determine	determine	VERB
biotropia-134	50	31	the	the	DET
biotropia-134	50	32	success	success	NOUN
biotropia-134	50	33	of	of	ADP
biotropia-134	50	34	transfection	transfection	NOUN
biotropia-134	50	35	method	method	NOUN
biotropia-134	50	36	.	.	PUNCT
biotropia-134	51	1	microinjection	microinjection	NOUN
biotropia-134	51	2	was	be	AUX
biotropia-134	51	3	conducted	conduct	VERB
biotropia-134	51	4	as	as	SCONJ
biotropia-134	51	5	described	describe	VERB
biotropia-134	51	6	previously	previously	ADV
biotropia-134	51	7	(	(	PUNCT
biotropia-134	51	8	alimuddin	alimuddin	VERB
biotropia-134	51	9	.	.	PUNCT
biotropia-134	52	1	2005	2005	NUM
biotropia-134	52	2	)	)	PUNCT
biotropia-134	52	3	microinjection	microinjection	NOUN
biotropia-134	52	4	was	be	AUX
biotropia-134	52	5	carried	carry	VERB
biotropia-134	52	6	out	out	ADP
biotropia-134	52	7	to	to	PART
biotropia-134	52	8	fertilized	fertilize	VERB
biotropia-134	52	9	grouper	grouper	NOUN
biotropia-134	52	10	eggs	egg	NOUN
biotropia-134	52	11	at	at	ADP
biotropia-134	52	12	2	2	NUM
biotropia-134	52	13	-	-	SYM
biotropia-134	52	14	4	4	NUM
biotropia-134	52	15	cell	cell	NOUN
biotropia-134	52	16	stage	stage	NOUN
biotropia-134	52	17	.	.	PUNCT
biotropia-134	53	1	a	a	DET
biotropia-134	53	2	microinjection	microinjection	NOUN
biotropia-134	53	3	plate	plate	NOUN
biotropia-134	53	4	that	that	PRON
biotropia-134	53	5	consists	consist	VERB
biotropia-134	53	6	of	of	ADP
biotropia-134	53	7	ten	ten	NUM
biotropia-134	53	8	grooves	groove	NOUN
biotropia-134	53	9	of	of	ADP
biotropia-134	53	10	3	3	NUM
biotropia-134	53	11	%	%	NOUN
biotropia-134	53	12	agarose	agarose	NOUN
biotropia-134	53	13	gel	gel	NOUN
biotropia-134	53	14	was	be	AUX
biotropia-134	53	15	used	use	VERB
biotropia-134	53	16	to	to	PART
biotropia-134	53	17	support	support	VERB
biotropia-134	53	18	embryos	embryo	NOUN
biotropia-134	53	19	when	when	SCONJ
biotropia-134	53	20	penetrated	penetrate	VERB
biotropia-134	53	21	by	by	ADP
biotropia-134	53	22	microinjection	microinjection	NOUN
biotropia-134	53	23	needle	needle	NOUN
biotropia-134	53	24	.	.	PUNCT
biotropia-134	54	1	humpback	humpback	NOUN
biotropia-134	54	2	grouper	grouper	NOUN
biotropia-134	54	3	embryos	embryo	NOUN
biotropia-134	54	4	were	be	AUX
biotropia-134	54	5	gently	gently	ADV
biotropia-134	54	6	transferred	transfer	VERB
biotropia-134	54	7	onto	onto	ADP
biotropia-134	54	8	the	the	DET
biotropia-134	54	9	groove	groove	NOUN
biotropia-134	54	10	of	of	ADP
biotropia-134	54	11	microinjection	microinjection	NOUN
biotropia-134	54	12	plate	plate	NOUN
biotropia-134	54	13	and	and	CCONJ
biotropia-134	54	14	blastodisc	blastodisc	NOUN
biotropia-134	54	15	should	should	AUX
biotropia-134	54	16	face	face	VERB
biotropia-134	54	17	the	the	DET
biotropia-134	54	18	direction	direction	NOUN
biotropia-134	54	19	of	of	ADP
biotropia-134	54	20	the	the	DET
biotropia-134	54	21	microinjection	microinjection	NOUN
biotropia-134	54	22	needle	needle	NOUN
biotropia-134	54	23	.	.	PUNCT
biotropia-134	55	1	dna	dna	PROPN
biotropia-134	55	2	solution	solution	NOUN
biotropia-134	55	3	at	at	ADP
biotropia-134	55	4	concentration	concentration	NOUN
biotropia-134	55	5	of	of	ADP
biotropia-134	55	6	25	25	NUM
biotropia-134	55	7	μg	μg	NOUN
biotropia-134	55	8	/	/	SYM
biotropia-134	55	9	ml	ml	PROPN
biotropia-134	55	10	was	be	AUX
biotropia-134	55	11	slowly	slowly	ADV
biotropia-134	55	12	injected	inject	VERB
biotropia-134	55	13	into	into	ADP
biotropia-134	55	14	blastodisc	blastodisc	NOUN
biotropia-134	55	15	.	.	PUNCT
biotropia-134	56	1	the	the	DET
biotropia-134	56	2	volume	volume	NOUN
biotropia-134	56	3	of	of	ADP
biotropia-134	56	4	injected	inject	VERB
biotropia-134	56	5	dna	dna	PROPN
biotropia-134	56	6	solution	solution	NOUN
biotropia-134	56	7	was	be	AUX
biotropia-134	56	8	about	about	ADV
biotropia-134	56	9	one	one	NUM
biotropia-134	56	10	-	-	PUNCT
biotropia-134	56	11	fifth	fifth	NOUN
biotropia-134	56	12	of	of	ADP
biotropia-134	56	13	the	the	DET
biotropia-134	56	14	blastodisc	blastodisc	NOUN
biotropia-134	56	15	volume	volume	NOUN
biotropia-134	56	16	(	(	PUNCT
biotropia-134	56	17	ath	ath	NOUN
biotropia-134	56	18	-	-	PUNCT
biotropia-134	56	19	thar	thar	NOUN
biotropia-134	56	20	2007	2007	NUM
biotropia-134	56	21	)	)	PUNCT
biotropia-134	56	22	.	.	PUNCT
biotropia-134	57	1	number	number	NOUN
biotropia-134	57	2	of	of	ADP
biotropia-134	57	3	embryos	embryo	NOUN
biotropia-134	57	4	that	that	PRON
biotropia-134	57	5	can	can	AUX
biotropia-134	57	6	be	be	AUX
biotropia-134	57	7	injected	inject	VERB
biotropia-134	57	8	by	by	ADP
biotropia-134	57	9	one	one	NUM
biotropia-134	57	10	microinjector	microinjector	NOUN
biotropia-134	57	11	in	in	ADP
biotropia-134	57	12	each	each	DET
biotropia-134	57	13	spawning	spawning	NOUN
biotropia-134	57	14	time	time	NOUN
biotropia-134	57	15	was	be	AUX
biotropia-134	57	16	60	60	NUM
biotropia-134	57	17	-	-	SYM
biotropia-134	57	18	100	100	NUM
biotropia-134	57	19	embryos	embryo	NOUN
biotropia-134	57	20	.	.	PUNCT
biotropia-134	58	1	three	three	NUM
biotropia-134	58	2	durations	duration	NOUN
biotropia-134	58	3	of	of	ADP
biotropia-134	58	4	injection	injection	NOUN
biotropia-134	58	5	(	(	PUNCT
biotropia-134	58	6	1	1	NUM
biotropia-134	58	7	,	,	PUNCT
biotropia-134	58	8	2	2	NUM
biotropia-134	58	9	and	and	CCONJ
biotropia-134	58	10	3	3	NUM
biotropia-134	58	11	seconds	second	NOUN
biotropia-134	58	12	)	)	PUNCT
biotropia-134	58	13	into	into	ADP
biotropia-134	58	14	each	each	DET
biotropia-134	58	15	embryo	embryo	NOUN
biotropia-134	58	16	were	be	AUX
biotropia-134	58	17	examined	examine	VERB
biotropia-134	58	18	to	to	PART
biotropia-134	58	19	obtain	obtain	VERB
biotropia-134	58	20	length	length	NOUN
biotropia-134	58	21	of	of	ADP
biotropia-134	58	22	injection	injection	NOUN
biotropia-134	58	23	time	time	NOUN
biotropia-134	58	24	resulting	result	VERB
biotropia-134	58	25	to	to	ADP
biotropia-134	58	26	higher	high	ADJ
biotropia-134	58	27	survival	survival	NOUN
biotropia-134	58	28	number	number	NOUN
biotropia-134	58	29	of	of	ADP
biotropia-134	58	30	injected	inject	VERB
biotropia-134	58	31	embryos	embryo	NOUN
biotropia-134	58	32	.	.	PUNCT
biotropia-134	59	1	electroporation	electroporation	NOUN
biotropia-134	59	2	is	be	AUX
biotropia-134	59	3	a	a	DET
biotropia-134	59	4	gene	gene	NOUN
biotropia-134	59	5	transfer	transfer	NOUN
biotropia-134	59	6	method	method	NOUN
biotropia-134	59	7	that	that	PRON
biotropia-134	59	8	utilizes	utilize	VERB
biotropia-134	59	9	a	a	DET
biotropia-134	59	10	series	series	NOUN
biotropia-134	59	11	of	of	ADP
biotropia-134	59	12	electric	electric	ADJ
biotropia-134	59	13	shock	shock	NOUN
biotropia-134	59	14	to	to	PART
biotropia-134	59	15	help	help	VERB
biotropia-134	59	16	foreign	foreign	ADJ
biotropia-134	59	17	dna	dna	NOUN
biotropia-134	59	18	to	to	PART
biotropia-134	59	19	enter	enter	VERB
biotropia-134	59	20	the	the	DET
biotropia-134	59	21	cell	cell	NOUN
biotropia-134	59	22	.	.	PUNCT
biotropia-134	60	1	electroporation	electroporation	NOUN
biotropia-134	60	2	was	be	AUX
biotropia-134	60	3	carried	carry	VERB
biotropia-134	60	4	out	out	ADP
biotropia-134	60	5	using	use	VERB
biotropia-134	60	6	gene	gene	NOUN
biotropia-134	60	7	pulser	pulser	PROPN
biotropia-134	60	8	xcell	xcell	PROPN
biotropia-134	60	9	electroporation	electroporation	NOUN
biotropia-134	60	10	system	system	NOUN
biotropia-134	60	11	(	(	PUNCT
biotropia-134	60	12	biorad	biorad	NOUN
biotropia-134	60	13	)	)	PUNCT
biotropia-134	60	14	by	by	ADP
biotropia-134	60	15	square	square	ADJ
biotropia-134	60	16	wave	wave	NOUN
biotropia-134	60	17	method	method	NOUN
biotropia-134	60	18	with	with	ADP
biotropia-134	60	19	parameters	parameter	NOUN
biotropia-134	60	20	of	of	ADP
biotropia-134	60	21	50	50	NUM
biotropia-134	60	22	volt	volt	NOUN
biotropia-134	60	23	,	,	PUNCT
biotropia-134	60	24	30	30	NUM
biotropia-134	60	25	ms	ms	NOUN
biotropia-134	60	26	of	of	ADP
biotropia-134	60	27	pulse	pulse	NOUN
biotropia-134	60	28	length	length	NOUN
biotropia-134	60	29	,	,	PUNCT
biotropia-134	60	30	5	5	NUM
biotropia-134	60	31	pulse	pulse	NOUN
biotropia-134	60	32	numbers	number	NOUN
biotropia-134	60	33	,	,	PUNCT
biotropia-134	60	34	0.1	0.1	NUM
biotropia-134	60	35	s	s	NOUN
biotropia-134	60	36	of	of	ADP
biotropia-134	60	37	pulse	pulse	NOUN
biotropia-134	60	38	interval	interval	NOUN
biotropia-134	60	39	,	,	PUNCT
biotropia-134	60	40	and	and	CCONJ
biotropia-134	60	41	using	use	VERB
biotropia-134	60	42	cuvettes	cuvette	NOUN
biotropia-134	60	43	of	of	ADP
biotropia-134	60	44	0.2	0.2	NUM
biotropia-134	60	45	and	and	CCONJ
biotropia-134	60	46	0.4	0.4	NUM
biotropia-134	60	47	cm	cm	NOUN
biotropia-134	60	48	gap	gap	NOUN
biotropia-134	60	49	size	size	NOUN
biotropia-134	60	50	.	.	PUNCT
biotropia-134	61	1	these	these	DET
biotropia-134	61	2	electroporation	electroporation	NOUN
biotropia-134	61	3	parameters	parameter	NOUN
biotropia-134	61	4	were	be	AUX
biotropia-134	61	5	obtained	obtain	VERB
biotropia-134	61	6	from	from	ADP
biotropia-134	61	7	the	the	DET
biotropia-134	61	8	preliminary	preliminary	ADJ
biotropia-134	61	9	study	study	NOUN
biotropia-134	61	10	.	.	PUNCT
biotropia-134	62	1	concentrations	concentration	NOUN
biotropia-134	62	2	of	of	ADP
biotropia-134	62	3	dna	dna	PROPN
biotropia-134	62	4	used	use	VERB
biotropia-134	62	5	in	in	ADP
biotropia-134	62	6	this	this	DET
biotropia-134	62	7	study	study	NOUN
biotropia-134	62	8	were	be	AUX
biotropia-134	62	9	5	5	NUM
biotropia-134	62	10	,	,	PUNCT
biotropia-134	62	11	10	10	NUM
biotropia-134	62	12	and	and	CCONJ
biotropia-134	62	13	20	20	NUM
biotropia-134	62	14	μg	μg	NOUN
biotropia-134	62	15	/	/	SYM
biotropia-134	62	16	ml	ml	NOUN
biotropia-134	62	17	,	,	PUNCT
biotropia-134	62	18	respectively	respectively	ADV
biotropia-134	62	19	.	.	PUNCT
biotropia-134	63	1	each	each	DET
biotropia-134	63	2	dna	dna	PROPN
biotropia-134	63	3	concentration	concentration	NOUN
biotropia-134	63	4	was	be	AUX
biotropia-134	63	5	mixed	mix	VERB
biotropia-134	63	6	with	with	ADP
biotropia-134	63	7	25	25	NUM
biotropia-134	63	8	μl	μl	INTJ
biotropia-134	63	9	grouper	grouper	NOUN
biotropia-134	63	10	sperm	sperm	NOUN
biotropia-134	63	11	and	and	CCONJ
biotropia-134	63	12	sodium	sodium	NOUN
biotropia-134	63	13	chloride	chloride	NOUN
biotropia-134	63	14	solution	solution	NOUN
biotropia-134	63	15	to	to	ADP
biotropia-134	63	16	final	final	ADJ
biotropia-134	63	17	volume	volume	NOUN
biotropia-134	63	18	of	of	ADP
biotropia-134	63	19	260	260	NUM
biotropia-134	63	20	μl	μl	NOUN
biotropia-134	63	21	.	.	PUNCT
biotropia-134	64	1	an	an	DET
biotropia-134	64	2	amount	amount	NOUN
biotropia-134	64	3	of	of	ADP
biotropia-134	64	4	100	100	NUM
biotropia-134	64	5	microliters	microliter	NOUN
biotropia-134	64	6	of	of	ADP
biotropia-134	64	7	electroporated	electroporate	VERB
biotropia-134	64	8	sperm	sperm	NOUN
biotropia-134	64	9	was	be	AUX
biotropia-134	64	10	used	use	VERB
biotropia-134	64	11	for	for	ADP
biotropia-134	64	12	dna	dna	NOUN
biotropia-134	64	13	analysis	analysis	NOUN
biotropia-134	64	14	using	use	VERB
biotropia-134	64	15	pcr	pcr	ADJ
biotropia-134	64	16	method	method	NOUN
biotropia-134	64	17	.	.	PUNCT
biotropia-134	65	1	transfected	transfecte	VERB
biotropia-134	65	2	and	and	CCONJ
biotropia-134	65	3	electroporated	electroporate	VERB
biotropia-134	65	4	sperm	sperm	NOUN
biotropia-134	65	5	solution	solution	NOUN
biotropia-134	65	6	was	be	AUX
biotropia-134	65	7	subjected	subject	VERB
biotropia-134	65	8	to	to	ADP
biotropia-134	65	9	centrifugation	centrifugation	NOUN
biotropia-134	65	10	for	for	ADP
biotropia-134	65	11	1	1	NUM
biotropia-134	65	12	min	min	NOUN
biotropia-134	65	13	at	at	ADP
biotropia-134	65	14	3000	3000	NUM
biotropia-134	65	15	rpm	rpm	NOUN
biotropia-134	65	16	to	to	PART
biotropia-134	65	17	remove	remove	VERB
biotropia-134	65	18	remaining	remaining	ADJ
biotropia-134	65	19	dna	dna	NOUN
biotropia-134	65	20	in	in	ADP
biotropia-134	65	21	solvent	solvent	NOUN
biotropia-134	65	22	and	and	CCONJ
biotropia-134	65	23	transfection	transfection	NOUN
biotropia-134	65	24	reagent	reagent	NOUN
biotropia-134	65	25	.	.	PUNCT
biotropia-134	66	1	sperm	sperm	NOUN
biotropia-134	66	2	was	be	AUX
biotropia-134	66	3	washed	wash	VERB
biotropia-134	66	4	two	two	NUM
biotropia-134	66	5	times	time	NOUN
biotropia-134	66	6	with	with	ADP
biotropia-134	66	7	200	200	NUM
biotropia-134	66	8	μl	μl	PRON
biotropia-134	66	9	physiological	physiological	ADJ
biotropia-134	66	10	salt	salt	NOUN
biotropia-134	66	11	solution	solution	NOUN
biotropia-134	66	12	before	before	ADP
biotropia-134	66	13	performing	perform	VERB
biotropia-134	66	14	in	in	ADP
biotropia-134	66	15	vitro	vitro	X
biotropia-134	66	16	in	in	ADP
biotropia-134	66	17	vivo	vivo	NOUN
biotropia-134	66	18	et	et	NOUN
biotropia-134	66	19	al	al	PROPN
biotropia-134	66	20	et	et	PROPN
biotropia-134	66	21	al	al	PROPN
biotropia-134	66	22	ratio	ratio	PROPN
biotropia-134	66	23	of	of	ADP
biotropia-134	66	24	1:2	1:2	NUM
biotropia-134	66	25	(	(	PUNCT
biotropia-134	66	26	1	1	NUM
biotropia-134	66	27	μg	μg	PROPN
biotropia-134	66	28	of	of	ADP
biotropia-134	66	29	dna	dna	PROPN
biotropia-134	66	30	:	:	PUNCT
biotropia-134	66	31	6	6	NUM
biotropia-134	66	32	μl	μl	ADP
biotropia-134	66	33	transfast	transfast	NOUN
biotropia-134	66	34	)	)	PUNCT
biotropia-134	66	35	was	be	AUX
biotropia-134	66	36	dissolved	dissolve	VERB
biotropia-134	66	37	in	in	ADP
biotropia-134	66	38	physiological	physiological	ADJ
biotropia-134	66	39	solution	solution	NOUN
biotropia-134	66	40	to	to	ADP
biotropia-134	66	41	final	final	ADJ
biotropia-134	66	42	volume	volume	NOUN
biotropia-134	66	43	of	of	ADP
biotropia-134	66	44	500	500	NUM
biotropia-134	66	45	μl	μl	ADJ
biotropia-134	66	46	,	,	PUNCT
biotropia-134	66	47	and	and	CCONJ
biotropia-134	66	48	then	then	ADV
biotropia-134	66	49	half	half	NOUN
biotropia-134	66	50	of	of	ADP
biotropia-134	66	51	16	16	NUM
biotropia-134	66	52	dna	dna	PROPN
biotropia-134	66	53	extraction	extraction	NOUN
biotropia-134	66	54	.	.	PUNCT
biotropia-134	67	1	sperm	sperm	NOUN
biotropia-134	67	2	and	and	CCONJ
biotropia-134	67	3	the	the	DET
biotropia-134	67	4	injected	inject	VERB
biotropia-134	67	5	embryos	embryo	NOUN
biotropia-134	67	6	were	be	AUX
biotropia-134	67	7	lyzed	lyze	VERB
biotropia-134	67	8	with	with	ADP
biotropia-134	67	9	500	500	NUM
biotropia-134	67	10	μl	μl	ADP
biotropia-134	67	11	of	of	ADP
biotropia-134	67	12	lysis	lysis	NOUN
biotropia-134	67	13	buffer	buffer	NOUN
biotropia-134	67	14	for	for	ADP
biotropia-134	67	15	10	10	NUM
biotropia-134	67	16	minutes	minute	NOUN
biotropia-134	67	17	at	at	ADP
biotropia-134	67	18	95ºc	95ºc	NOUN
biotropia-134	67	19	.	.	PUNCT
biotropia-134	68	1	dna	dna	PROPN
biotropia-134	68	2	was	be	AUX
biotropia-134	68	3	precipitated	precipitate	VERB
biotropia-134	68	4	using	use	VERB
biotropia-134	68	5	400	400	NUM
biotropia-134	68	6	μl	μl	ADP
biotropia-134	68	7	of	of	ADP
biotropia-134	68	8	ethanol	ethanol	NOUN
biotropia-134	68	9	96	96	NUM
biotropia-134	68	10	%	%	NOUN
biotropia-134	68	11	and	and	CCONJ
biotropia-134	68	12	then	then	ADV
biotropia-134	68	13	diluted	dilute	VERB
biotropia-134	68	14	with	with	ADP
biotropia-134	68	15	50	50	NUM
biotropia-134	68	16	μl	μl	PROPN
biotropia-134	68	17	depc	depc	PROPN
biotropia-134	68	18	water	water	PROPN
biotropia-134	68	19	.	.	PUNCT
biotropia-134	69	1	dna	dna	PROPN
biotropia-134	69	2	solution	solution	NOUN
biotropia-134	69	3	was	be	AUX
biotropia-134	69	4	stored	store	VERB
biotropia-134	69	5	at	at	ADP
biotropia-134	69	6	-20ºc	-20ºc	PROPN
biotropia-134	69	7	.	.	PUNCT
biotropia-134	70	1	pcr	pcr	ADJ
biotropia-134	70	2	analysis	analysis	NOUN
biotropia-134	70	3	was	be	AUX
biotropia-134	70	4	performed	perform	VERB
biotropia-134	70	5	in	in	ADP
biotropia-134	70	6	10	10	NUM
biotropia-134	70	7	μl	μl	ADP
biotropia-134	70	8	of	of	ADP
biotropia-134	70	9	1	1	NUM
biotropia-134	70	10	microgram	microgram	ADJ
biotropia-134	70	11	dna	dna	PROPN
biotropia-134	70	12	extracted	extract	VERB
biotropia-134	70	13	from	from	ADP
biotropia-134	70	14	sperm	sperm	NOUN
biotropia-134	70	15	and	and	CCONJ
biotropia-134	70	16	embryos	embryo	NOUN
biotropia-134	70	17	,	,	PUNCT
biotropia-134	70	18	1	1	NUM
biotropia-134	70	19	μl	μl	PRON
biotropia-134	70	20	primer	primer	NOUN
biotropia-134	70	21	forward	forward	ADV
biotropia-134	70	22	and	and	CCONJ
biotropia-134	70	23	reverse	reverse	VERB
biotropia-134	70	24	(	(	PUNCT
biotropia-134	70	25	10	10	NUM
biotropia-134	70	26	pmol	pmol	NOUN
biotropia-134	70	27	)	)	PUNCT
biotropia-134	70	28	,	,	PUNCT
biotropia-134	70	29	1	1	NUM
biotropia-134	70	30	μl	μl	ADP
biotropia-134	70	31	dntps	dntps	ADJ
biotropia-134	70	32	mix	mix	NOUN
biotropia-134	70	33	,	,	PUNCT
biotropia-134	70	34	1	1	NUM
biotropia-134	70	35	μl	μl	ADP
biotropia-134	70	36	ex	ex	X
biotropia-134	70	37	buffer	buffer	NOUN
biotropia-134	70	38	,	,	PUNCT
biotropia-134	70	39	0.05	0.05	NUM
biotropia-134	70	40	μl	μl	DET
biotropia-134	70	41	ex	ex	PRON
biotropia-134	70	42	polymerase	polymerase	NOUN
biotropia-134	70	43	(	(	PUNCT
biotropia-134	70	44	takara	takara	PROPN
biotropia-134	70	45	bio	bio	PROPN
biotropia-134	70	46	)	)	PUNCT
biotropia-134	70	47	and	and	CCONJ
biotropia-134	70	48	sdw	sdw	X
biotropia-134	70	49	to	to	PART
biotropia-134	70	50	reach	reach	VERB
biotropia-134	70	51	the	the	DET
biotropia-134	70	52	final	final	ADJ
biotropia-134	70	53	volume	volume	NOUN
biotropia-134	70	54	of	of	ADP
biotropia-134	70	55	10	10	NUM
biotropia-134	70	56	μl	μl	NOUN
biotropia-134	70	57	.	.	PUNCT
biotropia-134	71	1	forward	forward	ADV
biotropia-134	71	2	and	and	CCONJ
biotropia-134	71	3	reverse	reverse	VERB
biotropia-134	71	4	primers	primer	NOUN
biotropia-134	71	5	designed	design	VERB
biotropia-134	71	6	and	and	CCONJ
biotropia-134	71	7	used	use	VERB
biotropia-134	71	8	in	in	ADP
biotropia-134	71	9	this	this	DET
biotropia-134	71	10	study	study	NOUN
biotropia-134	71	11	were	be	AUX
biotropia-134	71	12	located	locate	VERB
biotropia-134	71	13	at	at	ADP
biotropia-134	71	14	3	3	NUM
biotropia-134	71	15	'	'	PART
biotropia-134	71	16	terminus	terminus	NOUN
biotropia-134	71	17	of	of	ADP
biotropia-134	71	18	ktbp	ktbp	NOUN
biotropia-134	71	19	promoter	promoter	NOUN
biotropia-134	71	20	(	(	PUNCT
biotropia-134	71	21	fbp2	fbp2	PROPN
biotropia-134	71	22	)	)	PUNCT
biotropia-134	71	23	and	and	CCONJ
biotropia-134	71	24	ktgh	ktgh	PROPN
biotropia-134	71	25	(	(	PUNCT
biotropia-134	71	26	rgh1	rgh1	ADJ
biotropia-134	71	27	)	)	PUNCT
biotropia-134	71	28	sequences	sequence	NOUN
biotropia-134	71	29	,	,	PUNCT
biotropia-134	71	30	respectively	respectively	ADV
biotropia-134	71	31	.	.	PUNCT
biotropia-134	72	1	the	the	DET
biotropia-134	72	2	sequence	sequence	NOUN
biotropia-134	72	3	of	of	ADP
biotropia-134	72	4	those	those	DET
biotropia-134	72	5	primers	primer	NOUN
biotropia-134	72	6	was	be	AUX
biotropia-134	72	7	5	5	NUM
biotropia-134	72	8	'	'	PART
biotropia-134	72	9	ttcatccagctgatgatt	ttcatccagctgatgatt	NOUN
biotropia-134	72	10	gccagatgtaac-3	gccagatgtaac-3	NOUN
biotropia-134	72	11	'	'	PUNCT
biotropia-134	72	12	and	and	CCONJ
biotropia-134	72	13	5'-agttggcttca	5'-agttggcttca	NUM
biotropia-134	72	14	-	-	PUNCT
biotropia-134	72	15	ggagagagtcgacattt	ggagagagtcgacattt	NOUN
biotropia-134	72	16	ag-3	ag-3	NOUN
biotropia-134	72	17	'	'	PUNCT
biotropia-134	72	18	.	.	PUNCT
biotropia-134	73	1	a	a	DET
biotropia-134	73	2	total	total	NOUN
biotropia-134	73	3	of	of	ADP
biotropia-134	73	4	35	35	NUM
biotropia-134	73	5	cycles	cycle	NOUN
biotropia-134	73	6	of	of	ADP
biotropia-134	73	7	denaturation	denaturation	NOUN
biotropia-134	73	8	for	for	ADP
biotropia-134	73	9	30	30	NUM
biotropia-134	73	10	s	s	NOUN
biotropia-134	73	11	at	at	ADP
biotropia-134	73	12	94ºc	94ºc	ADJ
biotropia-134	73	13	,	,	PUNCT
biotropia-134	73	14	annealing	anneal	VERB
biotropia-134	73	15	at	at	ADP
biotropia-134	73	16	62ºc	62ºc	ADJ
biotropia-134	73	17	for	for	ADP
biotropia-134	73	18	30	30	NUM
biotropia-134	73	19	s	s	NOUN
biotropia-134	73	20	,	,	PUNCT
biotropia-134	73	21	and	and	CCONJ
biotropia-134	73	22	extension	extension	NOUN
biotropia-134	73	23	at	at	ADP
biotropia-134	73	24	72ºc	72ºc	ADJ
biotropia-134	73	25	for	for	ADP
biotropia-134	73	26	1	1	NUM
biotropia-134	73	27	min	min	NOUN
biotropia-134	73	28	were	be	AUX
biotropia-134	73	29	conducted	conduct	VERB
biotropia-134	73	30	.	.	PUNCT
biotropia-134	74	1	two	two	NUM
biotropia-134	74	2	microliters	microliter	NOUN
biotropia-134	74	3	of	of	ADP
biotropia-134	74	4	pcr	pcr	ADJ
biotropia-134	74	5	product	product	NOUN
biotropia-134	74	6	was	be	AUX
biotropia-134	74	7	electrophoretically	electrophoretically	ADV
biotropia-134	74	8	separated	separate	VERB
biotropia-134	74	9	using	use	VERB
biotropia-134	74	10	1.5	1.5	NUM
biotropia-134	74	11	%	%	NOUN
biotropia-134	74	12	agarose	agarose	NOUN
biotropia-134	74	13	gel	gel	NOUN
biotropia-134	74	14	,	,	PUNCT
biotropia-134	74	15	stained	stain	VERB
biotropia-134	74	16	with	with	ADP
biotropia-134	74	17	ethidium	ethidium	ADJ
biotropia-134	74	18	bromide	bromide	NOUN
biotropia-134	74	19	,	,	PUNCT
biotropia-134	74	20	and	and	CCONJ
biotropia-134	74	21	photographed	photograph	VERB
biotropia-134	74	22	under	under	ADP
biotropia-134	74	23	ultraviolet	ultraviolet	ADJ
biotropia-134	74	24	light	light	NOUN
biotropia-134	74	25	.	.	PUNCT
biotropia-134	75	1	sperms	sperm	NOUN
biotropia-134	75	2	survived	survive	VERB
biotropia-134	75	3	when	when	SCONJ
biotropia-134	75	4	transfection	transfection	NOUN
biotropia-134	75	5	was	be	AUX
biotropia-134	75	6	conducted	conduct	VERB
biotropia-134	75	7	using	use	VERB
biotropia-134	75	8	transfast	transfast	NOUN
biotropia-134	75	9	reagent	reagent	NOUN
biotropia-134	75	10	in	in	ADP
biotropia-134	75	11	both	both	DET
biotropia-134	75	12	incubation	incubation	NOUN
biotropia-134	75	13	temperatures	temperature	NOUN
biotropia-134	75	14	,	,	PUNCT
biotropia-134	75	15	while	while	SCONJ
biotropia-134	75	16	contrarily	contrarily	ADV
biotropia-134	75	17	jetpei	jetpei	NOUN
biotropia-134	75	18	-	-	PUNCT
biotropia-134	75	19	transfected	transfecte	VERB
biotropia-134	75	20	sperm	sperm	NOUN
biotropia-134	75	21	died	die	VERB
biotropia-134	75	22	(	(	PUNCT
biotropia-134	75	23	table	table	NOUN
biotropia-134	75	24	1	1	NUM
biotropia-134	75	25	)	)	PUNCT
biotropia-134	75	26	.	.	PUNCT
biotropia-134	76	1	furthermore	furthermore	ADV
biotropia-134	76	2	,	,	PUNCT
biotropia-134	76	3	the	the	DET
biotropia-134	76	4	results	result	NOUN
biotropia-134	76	5	of	of	ADP
biotropia-134	76	6	pcr	pcr	ADJ
biotropia-134	76	7	analysis	analysis	NOUN
biotropia-134	76	8	showed	show	VERB
biotropia-134	76	9	that	that	SCONJ
biotropia-134	76	10	transfection	transfection	NOUN
biotropia-134	76	11	using	use	VERB
biotropia-134	76	12	transfast	transfast	NOUN
biotropia-134	76	13	reagent	reagent	NOUN
biotropia-134	76	14	for	for	ADP
biotropia-134	76	15	48	48	NUM
biotropia-134	76	16	hours	hour	NOUN
biotropia-134	76	17	at	at	ADP
biotropia-134	76	18	25	25	NUM
biotropia-134	76	19	c	c	NOUN
biotropia-134	76	20	incubation	incubation	NOUN
biotropia-134	76	21	temperature	temperature	NOUN
biotropia-134	76	22	allowed	allow	VERB
biotropia-134	76	23	foreign	foreign	ADJ
biotropia-134	76	24	dna	dna	NOUN
biotropia-134	76	25	to	to	PART
biotropia-134	76	26	incorporate	incorporate	VERB
biotropia-134	76	27	into	into	ADP
biotropia-134	76	28	sperm	sperm	NOUN
biotropia-134	76	29	(	(	PUNCT
biotropia-134	76	30	fig	fig	NOUN
biotropia-134	76	31	.	.	NOUN
biotropia-134	76	32	1	1	NUM
biotropia-134	76	33	lane	lane	NOUN
biotropia-134	76	34	2	2	NUM
biotropia-134	76	35	,	,	PUNCT
biotropia-134	76	36	table	table	NOUN
biotropia-134	76	37	1	1	NUM
biotropia-134	76	38	)	)	PUNCT
biotropia-134	76	39	.	.	PUNCT
biotropia-134	77	1	the	the	DET
biotropia-134	77	2	forward	forward	ADJ
biotropia-134	77	3	primer	primer	NOUN
biotropia-134	77	4	located	locate	VERB
biotropia-134	77	5	at	at	ADP
biotropia-134	77	6	5	5	NUM
biotropia-134	77	7	'	'	PART
biotropia-134	77	8	terminus	terminus	NOUN
biotropia-134	77	9	of	of	ADP
biotropia-134	77	10	ktbp	ktbp	NOUN
biotropia-134	77	11	(	(	PUNCT
biotropia-134	77	12	fbp1	fbp1	PROPN
biotropia-134	77	13	)	)	PUNCT
biotropia-134	77	14	and	and	CCONJ
biotropia-134	77	15	ap2	ap2	PROPN
biotropia-134	77	16	reverse	reverse	NOUN
biotropia-134	77	17	(	(	PUNCT
biotropia-134	77	18	5'-ctatagggcacgcgtggt-3	5'-ctatagggcacgcgtggt-3	NUM
biotropia-134	77	19	'	'	PUNCT
biotropia-134	77	20	)	)	PUNCT
biotropia-134	77	21	was	be	AUX
biotropia-134	77	22	used	use	VERB
biotropia-134	77	23	to	to	PART
biotropia-134	77	24	ensure	ensure	VERB
biotropia-134	77	25	that	that	SCONJ
biotropia-134	77	26	the	the	DET
biotropia-134	77	27	amplified	amplify	VERB
biotropia-134	77	28	dna	dna	NOUN
biotropia-134	77	29	product	product	NOUN
biotropia-134	77	30	was	be	AUX
biotropia-134	77	31	the	the	DET
biotropia-134	77	32	foreign	foreign	ADJ
biotropia-134	77	33	dna	dna	NOUN
biotropia-134	77	34	.	.	PUNCT
biotropia-134	78	1	pcr	pcr	ADJ
biotropia-134	78	2	product	product	NOUN
biotropia-134	78	3	using	use	VERB
biotropia-134	78	4	this	this	DET
biotropia-134	78	5	set	set	VERB
biotropia-134	78	6	primer	primer	NOUN
biotropia-134	78	7	was	be	AUX
biotropia-134	78	8	about	about	ADV
biotropia-134	78	9	2	2	NUM
biotropia-134	78	10	kb	kb	NOUN
biotropia-134	78	11	in	in	ADP
biotropia-134	78	12	size	size	NOUN
biotropia-134	78	13	(	(	PUNCT
biotropia-134	78	14	fig	fig	NOUN
biotropia-134	78	15	.	.	NOUN
biotropia-134	79	1	1	1	NUM
biotropia-134	79	2	lane	lane	NOUN
biotropia-134	79	3	1	1	NUM
biotropia-134	79	4	)	)	PUNCT
biotropia-134	79	5	.	.	PUNCT
biotropia-134	80	1	the	the	DET
biotropia-134	80	2	result	result	NOUN
biotropia-134	80	3	suggested	suggest	VERB
biotropia-134	80	4	that	that	SCONJ
biotropia-134	80	5	foreign	foreign	ADJ
biotropia-134	80	6	dna	dna	NOUN
biotropia-134	80	7	was	be	AUX
biotropia-134	80	8	incorporated	incorporate	VERB
biotropia-134	80	9	in	in	ADP
biotropia-134	80	10	sperm	sperm	NOUN
biotropia-134	80	11	.	.	PUNCT
biotropia-134	81	1	pcr	pcr	ADJ
biotropia-134	81	2	amplification	amplification	NOUN
biotropia-134	81	3	transfection	transfection	NOUN
biotropia-134	81	4	taq	taq	PROPN
biotropia-134	81	5	taq	taq	PROPN
biotropia-134	81	6	results	result	NOUN
biotropia-134	81	7	and	and	CCONJ
biotropia-134	81	8	discussion	discussion	NOUN
biotropia-134	81	9	in	in	ADP
biotropia-134	81	10	vitro	vitro	X
biotropia-134	81	11	o	o	NOUN
biotropia-134	81	12	biotropia	biotropia	NOUN
biotropia-134	81	13	vol	vol	NOUN
biotropia-134	81	14	.	.	PROPN
biotropia-134	82	1	18	18	NUM
biotropia-134	82	2	no	no	NOUN
biotropia-134	82	3	.	.	NOUN
biotropia-134	82	4	1	1	NUM
biotropia-134	82	5	,	,	PUNCT
biotropia-134	82	6	2011	2011	NUM
biotropia-134	82	7	table	table	NOUN
biotropia-134	82	8	1	1	NUM
biotropia-134	82	9	.	.	PUNCT
biotropia-134	83	1	survival	survival	NOUN
biotropia-134	83	2	and	and	CCONJ
biotropia-134	83	3	incorporation	incorporation	NOUN
biotropia-134	83	4	of	of	ADP
biotropia-134	83	5	foreign	foreign	ADJ
biotropia-134	83	6	dna	dna	NOUN
biotropia-134	83	7	into	into	ADP
biotropia-134	83	8	transfected	transfecte	VERB
biotropia-134	83	9	sperms	sperm	NOUN
biotropia-134	83	10	using	use	VERB
biotropia-134	83	11	transfast	transfast	NOUN
biotropia-134	83	12	and	and	CCONJ
biotropia-134	83	13	jetpei	jetpei	PROPN
biotropia-134	83	14	reagents	reagent	NOUN
biotropia-134	83	15	at	at	ADP
biotropia-134	83	16	17	17	NUM
biotropia-134	83	17	c	c	NOUN
biotropia-134	83	18	and	and	CCONJ
biotropia-134	83	19	25	25	NUM
biotropia-134	83	20	c	c	NOUN
biotropia-134	83	21	incubation	incubation	NOUN
biotropia-134	83	22	temperatures	temperature	NOUN
biotropia-134	83	23	.	.	PUNCT
biotropia-134	84	1	in	in	ADP
biotropia-134	84	2	vitro	vitro	X
biotropia-134	84	3	o	o	NOUN
biotropia-134	84	4	o	o	NOUN
biotropia-134	84	5	treatments	treatment	VERB
biotropia-134	84	6	duration	duration	NOUN
biotropia-134	84	7	of	of	ADP
biotropia-134	84	8	transfection	transfection	NOUN
biotropia-134	84	9	(	(	PUNCT
biotropia-134	84	10	hours	hour	NOUN
biotropia-134	84	11	)	)	PUNCT
biotropia-134	84	12	24	24	NUM
biotropia-134	84	13	48	48	NUM
biotropia-134	84	14	72	72	NUM
biotropia-134	84	15	incubation	incubation	NOUN
biotropia-134	84	16	temp	temp	NOUN
biotropia-134	84	17	.	.	PUNCT
biotropia-134	85	1	25	25	NUM
biotropia-134	85	2	oc	oc	PRON
biotropia-134	85	3	transfast	transfast	NOUN
biotropia-134	85	4	sperm	sperm	NOUN
biotropia-134	85	5	survived	survive	VERB
biotropia-134	85	6	,	,	PUNCT
biotropia-134	85	7	did	do	AUX
biotropia-134	85	8	not	not	PART
biotropia-134	85	9	carry	carry	VERB
biotropia-134	85	10	the	the	DET
biotropia-134	85	11	foreign	foreign	ADJ
biotropia-134	85	12	gene	gene	NOUN
biotropia-134	85	13	sperm	sperm	NOUN
biotropia-134	85	14	survived	survive	VERB
biotropia-134	85	15	and	and	CCONJ
biotropia-134	85	16	carry	carry	VERB
biotropia-134	85	17	the	the	DET
biotropia-134	85	18	foreign	foreign	ADJ
biotropia-134	85	19	gene	gene	NOUN
biotropia-134	85	20	sperm	sperm	NOUN
biotropia-134	85	21	survived	survive	VERB
biotropia-134	85	22	,	,	PUNCT
biotropia-134	85	23	did	do	AUX
biotropia-134	85	24	not	not	PART
biotropia-134	85	25	carry	carry	VERB
biotropia-134	85	26	the	the	DET
biotropia-134	85	27	foreign	foreign	ADJ
biotropia-134	85	28	gene	gene	NOUN
biotropia-134	85	29	jetpei	jetpei	PROPN
biotropia-134	85	30	sperm	sperm	NOUN
biotropia-134	85	31	died	die	VERB
biotropia-134	85	32	and	and	CCONJ
biotropia-134	85	33	did	do	AUX
biotropia-134	85	34	not	not	PART
biotropia-134	85	35	carry	carry	VERB
biotropia-134	85	36	the	the	DET
biotropia-134	85	37	foreign	foreign	ADJ
biotropia-134	85	38	gene	gene	NOUN
biotropia-134	85	39	sperm	sperm	NOUN
biotropia-134	85	40	died	die	VERB
biotropia-134	85	41	and	and	CCONJ
biotropia-134	85	42	did	do	AUX
biotropia-134	85	43	not	not	PART
biotropia-134	85	44	carry	carry	VERB
biotropia-134	85	45	the	the	DET
biotropia-134	85	46	foreign	foreign	ADJ
biotropia-134	85	47	gene	gene	NOUN
biotropia-134	85	48	sperm	sperm	NOUN
biotropia-134	85	49	died	die	VERB
biotropia-134	85	50	and	and	CCONJ
biotropia-134	85	51	did	do	AUX
biotropia-134	85	52	not	not	PART
biotropia-134	85	53	carry	carry	VERB
biotropia-134	85	54	the	the	DET
biotropia-134	85	55	foreign	foreign	ADJ
biotropia-134	85	56	gene	gene	NOUN
biotropia-134	85	57	control	control	NOUN
biotropia-134	85	58	sperm	sperm	NOUN
biotropia-134	85	59	survived	survive	VERB
biotropia-134	85	60	,	,	PUNCT
biotropia-134	85	61	no	no	PRON
biotropia-134	85	62	carry	carry	VERB
biotropia-134	85	63	the	the	DET
biotropia-134	85	64	foreign	foreign	ADJ
biotropia-134	85	65	gene	gene	NOUN
biotropia-134	85	66	incubation	incubation	NOUN
biotropia-134	85	67	temp	temp	NOUN
biotropia-134	85	68	.	.	PUNCT
biotropia-134	86	1	17	17	NUM
biotropia-134	86	2	oc	oc	PRON
biotropia-134	86	3	transfast	transfast	NOUN
biotropia-134	86	4	sperm	sperm	NOUN
biotropia-134	86	5	survived	survive	VERB
biotropia-134	86	6	,	,	PUNCT
biotropia-134	86	7	did	do	AUX
biotropia-134	86	8	not	not	PART
biotropia-134	86	9	carry	carry	VERB
biotropia-134	86	10	the	the	DET
biotropia-134	86	11	foreign	foreign	ADJ
biotropia-134	86	12	gene	gene	NOUN
biotropia-134	86	13	sperm	sperm	NOUN
biotropia-134	86	14	survived	survive	VERB
biotropia-134	86	15	,	,	PUNCT
biotropia-134	86	16	did	do	AUX
biotropia-134	86	17	not	not	PART
biotropia-134	86	18	carry	carry	VERB
biotropia-134	86	19	the	the	DET
biotropia-134	86	20	foreign	foreign	ADJ
biotropia-134	86	21	gene	gene	NOUN
biotropia-134	86	22	sperm	sperm	NOUN
biotropia-134	86	23	survived	survive	VERB
biotropia-134	86	24	,	,	PUNCT
biotropia-134	86	25	did	do	AUX
biotropia-134	86	26	not	not	PART
biotropia-134	86	27	carry	carry	VERB
biotropia-134	86	28	the	the	DET
biotropia-134	86	29	foreign	foreign	ADJ
biotropia-134	86	30	gene	gene	NOUN
biotropia-134	86	31	jetpei	jetpei	PROPN
biotropia-134	86	32	sperm	sperm	NOUN
biotropia-134	86	33	died	die	VERB
biotropia-134	86	34	and	and	CCONJ
biotropia-134	86	35	did	do	AUX
biotropia-134	86	36	not	not	PART
biotropia-134	86	37	carry	carry	VERB
biotropia-134	86	38	the	the	DET
biotropia-134	86	39	foreign	foreign	ADJ
biotropia-134	86	40	gene	gene	NOUN
biotropia-134	86	41	sperm	sperm	NOUN
biotropia-134	86	42	died	die	VERB
biotropia-134	86	43	and	and	CCONJ
biotropia-134	86	44	did	do	AUX
biotropia-134	86	45	not	not	PART
biotropia-134	86	46	carry	carry	VERB
biotropia-134	86	47	the	the	DET
biotropia-134	86	48	foreign	foreign	ADJ
biotropia-134	86	49	gene	gene	NOUN
biotropia-134	86	50	sperm	sperm	NOUN
biotropia-134	86	51	died	die	VERB
biotropia-134	86	52	and	and	CCONJ
biotropia-134	86	53	did	do	AUX
biotropia-134	86	54	not	not	PART
biotropia-134	86	55	carry	carry	VERB
biotropia-134	86	56	the	the	DET
biotropia-134	86	57	foreign	foreign	ADJ
biotropia-134	86	58	gene	gene	NOUN
biotropia-134	86	59	control	control	NOUN
biotropia-134	86	60	sperm	sperm	NOUN
biotropia-134	86	61	survived	survive	VERB
biotropia-134	86	62	,	,	PUNCT
biotropia-134	86	63	did	do	AUX
biotropia-134	86	64	not	not	PART
biotropia-134	86	65	carry	carry	VERB
biotropia-134	86	66	the	the	DET
biotropia-134	86	67	foreign	foreign	ADJ
biotropia-134	86	68	gene	gene	NOUN
biotropia-134	86	69	17	17	NUM
biotropia-134	86	70	in	in	ADP
biotropia-134	86	71	vivo	vivo	ADJ
biotropia-134	86	72	transfection	transfection	NOUN
biotropia-134	86	73	in	in	ADP
biotropia-134	86	74	vivo	vivo	NOUN
biotropia-134	86	75	transfection	transfection	NOUN
biotropia-134	86	76	was	be	AUX
biotropia-134	86	77	carried	carry	VERB
biotropia-134	86	78	out	out	ADP
biotropia-134	86	79	using	use	VERB
biotropia-134	86	80	ten	ten	NUM
biotropia-134	86	81	mature	mature	ADJ
biotropia-134	86	82	male	male	ADJ
biotropia-134	86	83	fish	fish	NOUN
biotropia-134	86	84	by	by	ADP
biotropia-134	86	85	injecting	inject	VERB
biotropia-134	86	86	dnatransfast	dnatransfast	ADJ
biotropia-134	86	87	complex	complex	NOUN
biotropia-134	86	88	to	to	ADP
biotropia-134	86	89	testis	testis	VERB
biotropia-134	86	90	through	through	ADP
biotropia-134	86	91	a	a	DET
biotropia-134	86	92	canulation	canulation	NOUN
biotropia-134	86	93	tube	tube	NOUN
biotropia-134	86	94	.	.	PUNCT
biotropia-134	87	1	this	this	DET
biotropia-134	87	2	technique	technique	NOUN
biotropia-134	87	3	was	be	AUX
biotropia-134	87	4	developed	develop	VERB
biotropia-134	87	5	,	,	PUNCT
biotropia-134	87	6	since	since	SCONJ
biotropia-134	87	7	fish	fish	NOUN
biotropia-134	87	8	could	could	AUX
biotropia-134	87	9	die	die	VERB
biotropia-134	87	10	when	when	SCONJ
biotropia-134	87	11	directly	directly	ADV
biotropia-134	87	12	injected	inject	VERB
biotropia-134	87	13	by	by	ADP
biotropia-134	87	14	syringe	syringe	NOUN
biotropia-134	87	15	into	into	ADP
biotropia-134	87	16	testis	testis	NOUN
biotropia-134	87	17	through	through	ADP
biotropia-134	87	18	urogenital	urogenital	ADJ
biotropia-134	87	19	pore	pore	NOUN
biotropia-134	87	20	was	be	AUX
biotropia-134	87	21	conducted	conduct	VERB
biotropia-134	87	22	.	.	PUNCT
biotropia-134	88	1	two	two	NUM
biotropia-134	88	2	out	out	ADP
biotropia-134	88	3	of	of	ADP
biotropia-134	88	4	ten	ten	NUM
biotropia-134	88	5	broodstocks	broodstock	NOUN
biotropia-134	88	6	contained	contain	VERB
biotropia-134	88	7	sperm	sperm	NOUN
biotropia-134	88	8	carrying	carry	VERB
biotropia-134	88	9	foreign	foreign	ADJ
biotropia-134	88	10	gene	gene	NOUN
biotropia-134	88	11	at	at	ADP
biotropia-134	88	12	36	36	NUM
biotropia-134	88	13	hours	hour	NOUN
biotropia-134	88	14	and	and	CCONJ
biotropia-134	88	15	60	60	NUM
biotropia-134	88	16	hours	hour	NOUN
biotropia-134	88	17	after	after	ADP
biotropia-134	88	18	transfection	transfection	NOUN
biotropia-134	88	19	,	,	PUNCT
biotropia-134	88	20	respectively	respectively	ADV
biotropia-134	88	21	.	.	PUNCT
biotropia-134	89	1	the	the	DET
biotropia-134	89	2	size	size	NOUN
biotropia-134	89	3	of	of	ADP
biotropia-134	89	4	dna	dna	PROPN
biotropia-134	89	5	band	band	NOUN
biotropia-134	89	6	of	of	ADP
biotropia-134	89	7	pcr	pcr	ADJ
biotropia-134	89	8	product	product	NOUN
biotropia-134	89	9	of	of	ADP
biotropia-134	89	10	dna	dna	PROPN
biotropia-134	89	11	from	from	ADP
biotropia-134	89	12	sperm	sperm	NOUN
biotropia-134	89	13	containing	contain	VERB
biotropia-134	89	14	foreign	foreign	ADJ
biotropia-134	89	15	gene	gene	NOUN
biotropia-134	89	16	was	be	AUX
biotropia-134	89	17	similar	similar	ADJ
biotropia-134	89	18	with	with	ADP
biotropia-134	89	19	that	that	PRON
biotropia-134	89	20	of	of	ADP
biotropia-134	89	21	pktba	pktba	NOUN
biotropia-134	89	22	-	-	PUNCT
biotropia-134	89	23	ktgh	ktgh	NOUN
biotropia-134	89	24	as	as	ADP
biotropia-134	89	25	template	template	NOUN
biotropia-134	89	26	(	(	PUNCT
biotropia-134	89	27	fig	fig	NOUN
biotropia-134	89	28	.	.	PUNCT
biotropia-134	90	1	2	2	NUM
biotropia-134	90	2	,	,	PUNCT
biotropia-134	90	3	indicated	indicate	VERB
biotropia-134	90	4	by	by	ADP
biotropia-134	90	5	arrow	arrow	NOUN
biotropia-134	90	6	head	head	NOUN
biotropia-134	90	7	)	)	PUNCT
biotropia-134	90	8	.	.	PUNCT
biotropia-134	91	1	kb	kb	PROPN
biotropia-134	91	2	10,0	10,0	NUM
biotropia-134	91	3	2,0	2,0	NUM
biotropia-134	91	4	1,0	1,0	NUM
biotropia-134	91	5	0,5	0,5	NUM
biotropia-134	91	6	0,1	0,1	NUM
biotropia-134	91	7	m	m	NOUN
biotropia-134	91	8	1	1	NUM
biotropia-134	91	9	2	2	NUM
biotropia-134	91	10	figure	figure	NOUN
biotropia-134	91	11	1	1	NUM
biotropia-134	91	12	.	.	PUNCT
biotropia-134	91	13	results	result	NOUN
biotropia-134	91	14	of	of	ADP
biotropia-134	91	15	pcr	pcr	ADJ
biotropia-134	91	16	analysis	analysis	NOUN
biotropia-134	91	17	with	with	ADP
biotropia-134	91	18	dna	dna	NOUN
biotropia-134	91	19	template	template	NOUN
biotropia-134	91	20	extracted	extract	VERB
biotropia-134	91	21	from	from	ADP
biotropia-134	91	22	transfected	transfecte	VERB
biotropia-134	91	23	sperm	sperm	NOUN
biotropia-134	91	24	using	use	VERB
biotropia-134	91	25	transfast	transfast	NOUN
biotropia-134	91	26	.	.	PUNCT
biotropia-134	92	1	lane	lane	NOUN
biotropia-134	92	2	1	1	NUM
biotropia-134	92	3	:	:	PUNCT
biotropia-134	92	4	pcr	pcr	ADJ
biotropia-134	92	5	amplification	amplification	NOUN
biotropia-134	92	6	product	product	NOUN
biotropia-134	92	7	using	use	VERB
biotropia-134	92	8	primers	primer	NOUN
biotropia-134	92	9	fbp-1	fbp-1	X
biotropia-134	92	10	(	(	PUNCT
biotropia-134	92	11	5'-gtgwgtgacgcyggaccaatc-3	5'-gtgwgtgacgcyggaccaatc-3	NUM
biotropia-134	92	12	'	'	PUNCT
biotropia-134	92	13	)	)	PUNCT
biotropia-134	92	14	and	and	CCONJ
biotropia-134	92	15	ap2	ap2	PROPN
biotropia-134	92	16	(	(	PUNCT
biotropia-134	92	17	5'-ctatagggcacgcgtggt-3	5'-ctatagggcacgcgtggt-3	NUM
biotropia-134	92	18	'	'	NUM
biotropia-134	92	19	)	)	PUNCT
biotropia-134	92	20	,	,	PUNCT
biotropia-134	92	21	lane	lane	NOUN
biotropia-134	92	22	2	2	NUM
biotropia-134	92	23	:	:	PUNCT
biotropia-134	92	24	pcr	pcr	ADJ
biotropia-134	92	25	amplification	amplification	NOUN
biotropia-134	92	26	product	product	NOUN
biotropia-134	92	27	using	use	VERB
biotropia-134	92	28	primers	primer	NOUN
biotropia-134	92	29	as	as	SCONJ
biotropia-134	92	30	described	describe	VERB
biotropia-134	92	31	in	in	ADP
biotropia-134	92	32	materials	material	NOUN
biotropia-134	92	33	and	and	CCONJ
biotropia-134	92	34	methods	method	NOUN
biotropia-134	92	35	.	.	PUNCT
biotropia-134	93	1	arrow	arrow	NOUN
biotropia-134	93	2	head	head	NOUN
biotropia-134	93	3	indicates	indicate	VERB
biotropia-134	93	4	pcr	pcr	ADJ
biotropia-134	93	5	product	product	NOUN
biotropia-134	93	6	of	of	ADP
biotropia-134	93	7	foreign	foreign	ADJ
biotropia-134	93	8	gh	gh	PROPN
biotropia-134	93	9	gene	gene	NOUN
biotropia-134	93	10	.	.	PUNCT
biotropia-134	94	1	transfection	transfection	NOUN
biotropia-134	94	2	was	be	AUX
biotropia-134	94	3	carried	carry	VERB
biotropia-134	94	4	out	out	ADP
biotropia-134	94	5	for	for	ADP
biotropia-134	94	6	48	48	NUM
biotropia-134	94	7	hours	hour	NOUN
biotropia-134	94	8	at	at	ADP
biotropia-134	94	9	25ºc	25ºc	NOUN
biotropia-134	94	10	.	.	PUNCT
biotropia-134	95	1	m	m	PROPN
biotropia-134	95	2	is	be	AUX
biotropia-134	95	3	2	2	NUM
biotropia-134	95	4	-	-	PUNCT
biotropia-134	95	5	log	log	NOUN
biotropia-134	95	6	ladder	ladder	NOUN
biotropia-134	95	7	dna	dna	PROPN
biotropia-134	95	8	marker	marker	NOUN
biotropia-134	95	9	(	(	PUNCT
biotropia-134	95	10	biolabs	biolabs	PROPN
biotropia-134	95	11	,	,	PUNCT
biotropia-134	95	12	inc	inc	PROPN
biotropia-134	95	13	.	.	PROPN
biotropia-134	95	14	,	,	PUNCT
biotropia-134	95	15	new	new	PROPN
biotropia-134	95	16	england	england	PROPN
biotropia-134	95	17	)	)	PUNCT
biotropia-134	95	18	.	.	PUNCT
biotropia-134	96	1	the	the	DET
biotropia-134	96	2	amplified	amplify	VERB
biotropia-134	96	3	fragment	fragment	NOUN
biotropia-134	96	4	is	be	AUX
biotropia-134	96	5	~700	~700	PROPN
biotropia-134	96	6	bp	bp	PROPN
biotropia-134	96	7	in	in	ADP
biotropia-134	96	8	size	size	NOUN
biotropia-134	96	9	.	.	PUNCT
biotropia-134	97	1	kb	kb	PROPN
biotropia-134	97	2	10,0	10,0	NUM
biotropia-134	97	3	3,0	3,0	NUM
biotropia-134	97	4	1,2	1,2	NUM
biotropia-134	97	5	1,0	1,0	NUM
biotropia-134	97	6	0,7	0,7	NUM
biotropia-134	97	7	0,5	0,5	NUM
biotropia-134	97	8	0,3	0,3	NUM
biotropia-134	97	9	0,1	0,1	NUM
biotropia-134	97	10	m	m	NOUN
biotropia-134	97	11	1	1	NUM
biotropia-134	97	12	2	2	NUM
biotropia-134	97	13	3	3	NUM
biotropia-134	97	14	4	4	NUM
biotropia-134	97	15	5	5	NUM
biotropia-134	97	16	6	6	NUM
biotropia-134	97	17	m	m	NOUN
biotropia-134	97	18	figure	figure	NOUN
biotropia-134	97	19	2	2	NUM
biotropia-134	97	20	.	.	NOUN
biotropia-134	97	21	results	result	NOUN
biotropia-134	97	22	of	of	ADP
biotropia-134	97	23	pcr	pcr	ADJ
biotropia-134	97	24	amplification	amplification	NOUN
biotropia-134	97	25	with	with	ADP
biotropia-134	97	26	dna	dna	PROPN
biotropia-134	97	27	that	that	PRON
biotropia-134	97	28	have	have	AUX
biotropia-134	97	29	been	be	AUX
biotropia-134	97	30	extracted	extract	VERB
biotropia-134	97	31	from	from	ADP
biotropia-134	97	32	transfected	transfecte	VERB
biotropia-134	97	33	sperm	sperm	NOUN
biotropia-134	97	34	at	at	ADP
biotropia-134	97	35	24	24	NUM
biotropia-134	97	36	and	and	CCONJ
biotropia-134	97	37	60	60	NUM
biotropia-134	97	38	hours	hour	NOUN
biotropia-134	97	39	post	post	NOUN
biotropia-134	97	40	transfection	transfection	NOUN
biotropia-134	97	41	(	(	PUNCT
biotropia-134	97	42	hpt	hpt	NOUN
biotropia-134	97	43	)	)	PUNCT
biotropia-134	97	44	.	.	PUNCT
biotropia-134	98	1	lane	lane	NOUN
biotropia-134	98	2	1	1	NUM
biotropia-134	98	3	and	and	CCONJ
biotropia-134	98	4	2	2	NUM
biotropia-134	98	5	:	:	PUNCT
biotropia-134	98	6	pcr	pcr	ADJ
biotropia-134	98	7	product	product	NOUN
biotropia-134	98	8	of	of	ADP
biotropia-134	98	9	dna	dna	PROPN
biotropia-134	98	10	from	from	ADP
biotropia-134	98	11	broodstock	broodstock	PROPN
biotropia-134	98	12	no	no	PROPN
biotropia-134	98	13	.	.	PROPN
biotropia-134	98	14	2919	2919	NUM
biotropia-134	98	15	at	at	ADP
biotropia-134	98	16	36	36	NUM
biotropia-134	98	17	hpt	hpt	NOUN
biotropia-134	98	18	and	and	CCONJ
biotropia-134	98	19	60	60	NUM
biotropia-134	98	20	hpt	hpt	NOUN
biotropia-134	98	21	,	,	PUNCT
biotropia-134	98	22	respectively	respectively	ADV
biotropia-134	98	23	.	.	PUNCT
biotropia-134	99	1	lane	lane	NOUN
biotropia-134	99	2	3	3	NUM
biotropia-134	99	3	and	and	CCONJ
biotropia-134	99	4	4	4	NUM
biotropia-134	99	5	:	:	PUNCT
biotropia-134	99	6	pcr	pcr	ADJ
biotropia-134	99	7	product	product	NOUN
biotropia-134	99	8	of	of	ADP
biotropia-134	99	9	dna	dna	PROPN
biotropia-134	99	10	from	from	ADP
biotropia-134	99	11	broodstock	broodstock	PROPN
biotropia-134	99	12	no	no	PROPN
biotropia-134	99	13	.	.	PROPN
biotropia-134	99	14	2935	2935	NUM
biotropia-134	99	15	at	at	ADP
biotropia-134	99	16	36	36	NUM
biotropia-134	99	17	hpt	hpt	NOUN
biotropia-134	99	18	and	and	CCONJ
biotropia-134	99	19	60	60	NUM
biotropia-134	99	20	hpt	hpt	NOUN
biotropia-134	99	21	,	,	PUNCT
biotropia-134	99	22	respectively	respectively	ADV
biotropia-134	99	23	.	.	PUNCT
biotropia-134	100	1	lane	lane	PROPN
biotropia-134	100	2	5	5	NUM
biotropia-134	100	3	:	:	PUNCT
biotropia-134	100	4	pcr	pcr	ADJ
biotropia-134	100	5	product	product	NOUN
biotropia-134	100	6	using	use	VERB
biotropia-134	100	7	pktba	pktba	NOUN
biotropia-134	100	8	-	-	PUNCT
biotropia-134	100	9	ktgh	ktgh	NOUN
biotropia-134	100	10	as	as	ADP
biotropia-134	100	11	template	template	NOUN
biotropia-134	100	12	,	,	PUNCT
biotropia-134	100	13	lane	lane	NOUN
biotropia-134	100	14	6	6	NUM
biotropia-134	100	15	:	:	PUNCT
biotropia-134	100	16	product	product	NOUN
biotropia-134	100	17	of	of	ADP
biotropia-134	100	18	pcr	pcr	PROPN
biotropia-134	100	19	with	with	ADP
biotropia-134	100	20	no	no	DET
biotropia-134	100	21	dna	dna	NOUN
biotropia-134	100	22	template	template	NOUN
biotropia-134	100	23	.	.	PUNCT
biotropia-134	101	1	m	m	PROPN
biotropia-134	101	2	is	be	AUX
biotropia-134	101	3	2	2	NUM
biotropia-134	101	4	-	-	PUNCT
biotropia-134	101	5	log	log	NOUN
biotropia-134	101	6	ladder	ladder	NOUN
biotropia-134	101	7	dna	dna	PROPN
biotropia-134	101	8	marker	marker	NOUN
biotropia-134	101	9	(	(	PUNCT
biotropia-134	101	10	biolabs	biolabs	PROPN
biotropia-134	101	11	,	,	PUNCT
biotropia-134	101	12	inc	inc	PROPN
biotropia-134	101	13	.	.	PROPN
biotropia-134	101	14	,	,	PUNCT
biotropia-134	101	15	new	new	PROPN
biotropia-134	101	16	england	england	PROPN
biotropia-134	101	17	)	)	PUNCT
biotropia-134	101	18	.	.	PUNCT
biotropia-134	102	1	in	in	ADP
biotropia-134	102	2	vivo	vivo	ADJ
biotropia-134	102	3	gene	gene	NOUN
biotropia-134	102	4	transfer	transfer	NOUN
biotropia-134	102	5	method	method	NOUN
biotropia-134	102	6	in	in	ADP
biotropia-134	102	7	humpback	humpback	NOUN
biotropia-134	102	8	grouper	grouper	NOUN
biotropia-134	102	9	alimuddin	alimuddin	PROPN
biotropia-134	102	10	.et	.et	PUNCT
biotropia-134	103	1	al	al	PROPN
biotropia-134	103	2	18	18	NUM
biotropia-134	103	3	biotropia	biotropia	ADJ
biotropia-134	103	4	vol	vol	NOUN
biotropia-134	103	5	.	.	PROPN
biotropia-134	103	6	18	18	NUM
biotropia-134	103	7	no	no	NOUN
biotropia-134	103	8	.	.	NOUN
biotropia-134	103	9	1	1	NUM
biotropia-134	103	10	,	,	PUNCT
biotropia-134	103	11	2011	2011	NUM
biotropia-134	103	12	microinjection	microinjection	NOUN
biotropia-134	103	13	high	high	ADJ
biotropia-134	103	14	survival	survival	NOUN
biotropia-134	103	15	rate	rate	NOUN
biotropia-134	103	16	of	of	ADP
biotropia-134	103	17	uninjected	uninjected	ADJ
biotropia-134	103	18	embryos	embryo	NOUN
biotropia-134	103	19	(	(	PUNCT
biotropia-134	103	20	92	92	NUM
biotropia-134	103	21	%	%	NOUN
biotropia-134	103	22	)	)	PUNCT
biotropia-134	103	23	indicated	indicate	VERB
biotropia-134	103	24	that	that	SCONJ
biotropia-134	103	25	a	a	DET
biotropia-134	103	26	high	high	ADJ
biotropia-134	103	27	quality	quality	NOUN
biotropia-134	103	28	of	of	ADP
biotropia-134	103	29	fertilized	fertilize	VERB
biotropia-134	103	30	eggs	egg	NOUN
biotropia-134	103	31	was	be	AUX
biotropia-134	103	32	used	use	VERB
biotropia-134	103	33	for	for	ADP
biotropia-134	103	34	microinjection	microinjection	NOUN
biotropia-134	103	35	(	(	PUNCT
biotropia-134	103	36	table	table	NOUN
biotropia-134	103	37	2	2	NUM
biotropia-134	103	38	)	)	PUNCT
biotropia-134	103	39	.	.	PUNCT
biotropia-134	104	1	percentage	percentage	NOUN
biotropia-134	104	2	of	of	ADP
biotropia-134	104	3	survived	survive	VERB
biotropia-134	104	4	microinjected	microinjecte	VERB
biotropia-134	104	5	embryos	embryo	NOUN
biotropia-134	104	6	14	14	NUM
biotropia-134	104	7	hours	hour	NOUN
biotropia-134	104	8	after	after	SCONJ
biotropia-134	104	9	injection	injection	NOUN
biotropia-134	104	10	decreased	decrease	VERB
biotropia-134	104	11	with	with	ADP
biotropia-134	104	12	the	the	DET
biotropia-134	104	13	increase	increase	NOUN
biotropia-134	104	14	in	in	ADP
biotropia-134	104	15	duration	duration	NOUN
biotropia-134	104	16	of	of	ADP
biotropia-134	104	17	dna	dna	PROPN
biotropia-134	104	18	injection	injection	NOUN
biotropia-134	104	19	into	into	ADP
biotropia-134	104	20	blastodisc	blastodisc	NOUN
biotropia-134	104	21	(	(	PUNCT
biotropia-134	104	22	table	table	NOUN
biotropia-134	104	23	2	2	NUM
biotropia-134	104	24	)	)	PUNCT
biotropia-134	104	25	.	.	PUNCT
biotropia-134	105	1	increasing	increase	VERB
biotropia-134	105	2	the	the	DET
biotropia-134	105	3	duration	duration	NOUN
biotropia-134	105	4	of	of	ADP
biotropia-134	105	5	injection	injection	NOUN
biotropia-134	105	6	into	into	ADP
biotropia-134	105	7	blastodisc	blastodisc	NOUN
biotropia-134	105	8	increased	increase	VERB
biotropia-134	105	9	dna	dna	PROPN
biotropia-134	105	10	volume	volume	NOUN
biotropia-134	105	11	entering	enter	VERB
biotropia-134	105	12	the	the	DET
biotropia-134	105	13	cytoplasm	cytoplasm	NOUN
biotropia-134	105	14	.	.	PUNCT
biotropia-134	106	1	thus	thus	ADV
biotropia-134	106	2	,	,	PUNCT
biotropia-134	106	3	high	high	ADJ
biotropia-134	106	4	dna	dna	NOUN
biotropia-134	106	5	volume	volume	NOUN
biotropia-134	106	6	in	in	ADP
biotropia-134	106	7	cytoplasm	cytoplasm	NOUN
biotropia-134	106	8	of	of	ADP
biotropia-134	106	9	blastodisc	blastodisc	NOUN
biotropia-134	106	10	probably	probably	ADV
biotropia-134	106	11	caused	cause	VERB
biotropia-134	106	12	embryos	embryo	NOUN
biotropia-134	106	13	to	to	PART
biotropia-134	106	14	die	die	VERB
biotropia-134	106	15	.	.	PUNCT
biotropia-134	107	1	similar	similar	ADJ
biotropia-134	107	2	result	result	NOUN
biotropia-134	107	3	has	have	AUX
biotropia-134	107	4	been	be	AUX
biotropia-134	107	5	obtained	obtain	VERB
biotropia-134	107	6	for	for	ADP
biotropia-134	107	7	catfish	catfish	NOUN
biotropia-134	107	8	(	(	PUNCT
biotropia-134	107	9	at	at	ADP
biotropia-134	107	10	-	-	PUNCT
biotropia-134	107	11	thar	thar	NOUN
biotropia-134	107	12	2007	2007	NUM
biotropia-134	107	13	)	)	PUNCT
biotropia-134	107	14	.	.	PUNCT
biotropia-134	108	1	in	in	ADP
biotropia-134	108	2	addition	addition	NOUN
biotropia-134	108	3	,	,	PUNCT
biotropia-134	108	4	embryo	embryo	NOUN
biotropia-134	108	5	may	may	AUX
biotropia-134	108	6	also	also	ADV
biotropia-134	108	7	die	die	VERB
biotropia-134	108	8	when	when	SCONJ
biotropia-134	108	9	injection	injection	NOUN
biotropia-134	108	10	is	be	AUX
biotropia-134	108	11	too	too	ADV
biotropia-134	108	12	deep	deep	ADJ
biotropia-134	108	13	into	into	ADP
biotropia-134	108	14	yolksac	yolksac	NOUN
biotropia-134	108	15	(	(	PUNCT
biotropia-134	108	16	at	at	ADP
biotropia-134	108	17	-	-	PUNCT
biotropia-134	108	18	thar	thar	NOUN
biotropia-134	108	19	2007	2007	NUM
biotropia-134	108	20	)	)	PUNCT
biotropia-134	108	21	.	.	PUNCT
biotropia-134	109	1	this	this	PRON
biotropia-134	109	2	might	might	AUX
biotropia-134	109	3	also	also	ADV
biotropia-134	109	4	contribute	contribute	VERB
biotropia-134	109	5	to	to	ADP
biotropia-134	109	6	the	the	DET
biotropia-134	109	7	decrease	decrease	NOUN
biotropia-134	109	8	in	in	ADP
biotropia-134	109	9	survival	survival	NOUN
biotropia-134	109	10	rate	rate	NOUN
biotropia-134	109	11	of	of	ADP
biotropia-134	109	12	injected	inject	VERB
biotropia-134	109	13	humpback	humpback	NOUN
biotropia-134	109	14	grouper	grouper	NOUN
biotropia-134	109	15	embryos	embryo	NOUN
biotropia-134	109	16	.	.	PUNCT
biotropia-134	110	1	as	as	ADP
biotropia-134	110	2	blastodisc	blastodisc	NOUN
biotropia-134	110	3	of	of	ADP
biotropia-134	110	4	humpback	humpback	NOUN
biotropia-134	110	5	grouper	grouper	NOUN
biotropia-134	110	6	is	be	AUX
biotropia-134	110	7	very	very	ADV
biotropia-134	110	8	thin	thin	ADJ
biotropia-134	110	9	,	,	PUNCT
biotropia-134	110	10	it	it	PRON
biotropia-134	110	11	is	be	AUX
biotropia-134	110	12	highly	highly	ADV
biotropia-134	110	13	possible	possible	ADJ
biotropia-134	110	14	that	that	SCONJ
biotropia-134	110	15	the	the	DET
biotropia-134	110	16	microinjection	microinjection	NOUN
biotropia-134	110	17	needle	needle	NOUN
biotropia-134	110	18	enters	enter	VERB
biotropia-134	110	19	the	the	DET
biotropia-134	110	20	yolksac	yolksac	NOUN
biotropia-134	110	21	.	.	PUNCT
biotropia-134	111	1	furthermore	furthermore	ADV
biotropia-134	111	2	,	,	PUNCT
biotropia-134	111	3	twenty	twenty	NUM
biotropia-134	111	4	microinjected	microinjecte	VERB
biotropia-134	111	5	embryos	embryo	NOUN
biotropia-134	111	6	for	for	ADP
biotropia-134	111	7	each	each	DET
biotropia-134	111	8	treatment	treatment	NOUN
biotropia-134	111	9	at	at	ADP
biotropia-134	111	10	14	14	NUM
biotropia-134	111	11	hours	hour	NOUN
biotropia-134	111	12	post	post	NOUN
biotropia-134	111	13	injection	injection	NOUN
biotropia-134	111	14	were	be	AUX
biotropia-134	111	15	pooled	pool	VERB
biotropia-134	111	16	into	into	ADP
biotropia-134	111	17	a	a	DET
biotropia-134	111	18	tube	tube	NOUN
biotropia-134	111	19	for	for	ADP
biotropia-134	111	20	dna	dna	PROPN
biotropia-134	111	21	analysis	analysis	NOUN
biotropia-134	111	22	.	.	PUNCT
biotropia-134	112	1	pcr	pcr	ADJ
biotropia-134	112	2	amplification	amplification	NOUN
biotropia-134	112	3	product	product	NOUN
biotropia-134	112	4	showed	show	VERB
biotropia-134	112	5	that	that	SCONJ
biotropia-134	112	6	all	all	DET
biotropia-134	112	7	microinjected	microinjecte	VERB
biotropia-134	112	8	embryos	embryo	NOUN
biotropia-134	112	9	group	group	NOUN
biotropia-134	112	10	contained	contain	VERB
biotropia-134	112	11	foreign	foreign	ADJ
biotropia-134	112	12	dna	dna	NOUN
biotropia-134	112	13	(	(	PUNCT
biotropia-134	112	14	fig	fig	NOUN
biotropia-134	112	15	.	.	PUNCT
biotropia-134	113	1	3	3	NUM
biotropia-134	113	2	)	)	PUNCT
biotropia-134	113	3	.	.	PUNCT
biotropia-134	114	1	this	this	PRON
biotropia-134	114	2	suggested	suggest	VERB
biotropia-134	114	3	that	that	SCONJ
biotropia-134	114	4	foreign	foreign	ADJ
biotropia-134	114	5	dna	dna	NOUN
biotropia-134	114	6	was	be	AUX
biotropia-134	114	7	transferred	transfer	VERB
biotropia-134	114	8	into	into	ADP
biotropia-134	114	9	blastodisc	blastodisc	NOUN
biotropia-134	114	10	.	.	PUNCT
biotropia-134	115	1	table	table	NOUN
biotropia-134	115	2	2	2	NUM
biotropia-134	115	3	.	.	X
biotropia-134	115	4	number	number	NOUN
biotropia-134	115	5	and	and	CCONJ
biotropia-134	115	6	percentage	percentage	NOUN
biotropia-134	115	7	of	of	ADP
biotropia-134	115	8	developed	develop	VERB
biotropia-134	115	9	embryos	embryo	NOUN
biotropia-134	115	10	after	after	ADP
biotropia-134	115	11	injection	injection	NOUN
biotropia-134	115	12	of	of	ADP
biotropia-134	115	13	25	25	NUM
biotropia-134	115	14	μg	μg	NOUN
biotropia-134	115	15	/	/	SYM
biotropia-134	115	16	ml	ml	NOUN
biotropia-134	115	17	at	at	ADP
biotropia-134	115	18	1	1	NUM
biotropia-134	115	19	,	,	PUNCT
biotropia-134	115	20	2	2	NUM
biotropia-134	115	21	and	and	CCONJ
biotropia-134	115	22	3	3	NUM
biotropia-134	115	23	seconds	second	NOUN
biotropia-134	115	24	.	.	PUNCT
biotropia-134	116	1	treatment	treatment	NOUN
biotropia-134	116	2	repetition	repetition	NOUN
biotropia-134	116	3	no	no	INTJ
biotropia-134	116	4	.	.	PUNCT
biotropia-134	116	5	developed	develop	VERB
biotropia-134	116	6	embryos	embryo	NOUN
biotropia-134	116	7	undeveloped	undeveloped	ADJ
biotropia-134	116	8	embryos	embryo	NOUN
biotropia-134	116	9	percentage	percentage	NOUN
biotropia-134	116	10	developed	develop	VERB
biotropia-134	116	11	embryos	embryo	NOUN
biotropia-134	116	12	1	1	NUM
biotropia-134	116	13	second	second	ADJ
biotropia-134	116	14	1	1	NUM
biotropia-134	116	15	42	42	NUM
biotropia-134	116	16	2	2	NUM
biotropia-134	116	17	95,5	95,5	NUM
biotropia-134	116	18	2	2	NUM
biotropia-134	116	19	41	41	NUM
biotropia-134	116	20	14	14	NUM
biotropia-134	116	21	74,5	74,5	NOUN
biotropia-134	117	1	mean	mean	VERB
biotropia-134	117	2	85.0±14.8	85.0±14.8	NUM
biotropia-134	117	3	2	2	NUM
biotropia-134	117	4	second	second	ADJ
biotropia-134	117	5	1	1	NUM
biotropia-134	117	6	1	1	NUM
biotropia-134	117	7	18	18	NUM
biotropia-134	117	8	5,3	5,3	NUM
biotropia-134	117	9	2	2	NUM
biotropia-134	117	10	3	3	NUM
biotropia-134	117	11	42	42	NUM
biotropia-134	117	12	6,7	6,7	NUM
biotropia-134	117	13	mean	mean	NOUN
biotropia-134	117	14	6.1±1.0	6.1±1.0	NUM
biotropia-134	117	15	3	3	NUM
biotropia-134	117	16	second	second	NOUN
biotropia-134	117	17	1	1	NUM
biotropia-134	117	18	0	0	NUM
biotropia-134	117	19	31	31	NUM
biotropia-134	117	20	0,0	0,0	NUM
biotropia-134	117	21	2	2	NUM
biotropia-134	117	22	0	0	NUM
biotropia-134	117	23	105	105	NUM
biotropia-134	117	24	0,0	0,0	NUM
biotropia-134	117	25	mean	mean	ADJ
biotropia-134	117	26	control	control	NOUN
biotropia-134	117	27	508	508	NUM
biotropia-134	117	28	39	39	NUM
biotropia-134	117	29	0.0±0.0	0.0±0.0	NUM
biotropia-134	117	30	92,9	92,9	NUM
biotropia-134	117	31	electroporation	electroporation	NOUN
biotropia-134	117	32	in	in	ADP
biotropia-134	117	33	this	this	DET
biotropia-134	117	34	study	study	NOUN
biotropia-134	117	35	,	,	PUNCT
biotropia-134	117	36	application	application	NOUN
biotropia-134	117	37	of	of	ADP
biotropia-134	117	38	electroporation	electroporation	NOUN
biotropia-134	117	39	method	method	NOUN
biotropia-134	117	40	for	for	ADP
biotropia-134	117	41	transferring	transfer	VERB
biotropia-134	117	42	the	the	DET
biotropia-134	117	43	gene	gene	NOUN
biotropia-134	117	44	revealed	reveal	VERB
biotropia-134	117	45	that	that	SCONJ
biotropia-134	117	46	high	high	ADJ
biotropia-134	117	47	percentage	percentage	NOUN
biotropia-134	117	48	of	of	ADP
biotropia-134	117	49	electroporated	electroporate	VERB
biotropia-134	117	50	sperm	sperm	NOUN
biotropia-134	117	51	was	be	AUX
biotropia-134	117	52	motile	motile	ADJ
biotropia-134	117	53	(	(	PUNCT
biotropia-134	117	54	table	table	NOUN
biotropia-134	117	55	3	3	NUM
biotropia-134	117	56	)	)	PUNCT
biotropia-134	117	57	.	.	PUNCT
biotropia-134	118	1	there	there	PRON
biotropia-134	118	2	was	be	VERB
biotropia-134	118	3	no	no	DET
biotropia-134	118	4	difference	difference	NOUN
biotropia-134	118	5	in	in	ADP
biotropia-134	118	6	sperm	sperm	NOUN
biotropia-134	118	7	motility	motility	NOUN
biotropia-134	118	8	of	of	ADP
biotropia-134	118	9	treated	treat	VERB
biotropia-134	118	10	and	and	CCONJ
biotropia-134	118	11	untreated	untreated	ADJ
biotropia-134	118	12	dna	dna	PROPN
biotropia-134	118	13	i.e.	i.e.	X
biotropia-134	118	14	during	during	ADP
biotropia-134	118	15	5	5	NUM
biotropia-134	118	16	-	-	SYM
biotropia-134	118	17	10	10	NUM
biotropia-134	118	18	minutes	minute	NOUN
biotropia-134	118	19	in	in	ADP
biotropia-134	118	20	water	water	NOUN
biotropia-134	118	21	.	.	PUNCT
biotropia-134	119	1	this	this	DET
biotropia-134	119	2	result	result	NOUN
biotropia-134	119	3	suggested	suggest	VERB
biotropia-134	119	4	that	that	SCONJ
biotropia-134	119	5	electroporated	electroporate	VERB
biotropia-134	119	6	sperm	sperm	NOUN
biotropia-134	119	7	could	could	AUX
biotropia-134	119	8	fertilize	fertilize	VERB
biotropia-134	119	9	the	the	DET
biotropia-134	119	10	eggs	egg	NOUN
biotropia-134	119	11	as	as	ADP
biotropia-134	119	12	in	in	ADP
biotropia-134	119	13	untreated	untreated	ADJ
biotropia-134	119	14	sperm	sperm	NOUN
biotropia-134	119	15	.	.	PUNCT
biotropia-134	120	1	percentage	percentage	NOUN
biotropia-134	120	2	of	of	ADP
biotropia-134	120	3	motile	motile	ADJ
biotropia-134	120	4	slight	slight	ADJ
biotropia-134	120	5	difference	difference	NOUN
biotropia-134	120	6	of	of	ADP
biotropia-134	120	7	water	water	NOUN
biotropia-134	120	8	quantity	quantity	NOUN
biotropia-134	120	9	in	in	ADP
biotropia-134	120	10	dna	dna	PROPN
biotropia-134	120	11	solution	solution	NOUN
biotropia-134	120	12	might	might	AUX
biotropia-134	120	13	take	take	VERB
biotropia-134	120	14	account	account	NOUN
biotropia-134	120	15	to	to	PART
biotropia-134	120	16	reduce	reduce	VERB
biotropia-134	120	17	percentage	percentage	NOUN
biotropia-134	120	18	of	of	ADP
biotropia-134	120	19	motile	motile	NOUN
biotropia-134	120	20	ml	ml	ADP
biotropia-134	120	21	dna	dna	PROPN
biotropia-134	120	22	concentration	concentration	NOUN
biotropia-134	120	23	.	.	PUNCT
biotropia-134	121	1	furthermore	furthermore	ADV
biotropia-134	121	2	,	,	PUNCT
biotropia-134	121	3	droop	droop	NOUN
biotropia-134	121	4	value	value	NOUN
biotropia-134	121	5	when	when	SCONJ
biotropia-134	121	6	using	use	VERB
biotropia-134	121	7	0.2	0.2	NUM
biotropia-134	121	8	cm	cm	NOUN
biotropia-134	121	9	cuvette	cuvette	NOUN
biotropia-134	121	10	was	be	AUX
biotropia-134	121	11	slightly	slightly	ADV
biotropia-134	121	12	higher	high	ADJ
biotropia-134	121	13	(	(	PUNCT
biotropia-134	121	14	6	6	NUM
biotropia-134	121	15	-	-	SYM
biotropia-134	121	16	12	12	NUM
biotropia-134	121	17	%	%	NOUN
biotropia-134	121	18	)	)	PUNCT
biotropia-134	121	19	compared	compare	VERB
biotropia-134	121	20	with	with	ADP
biotropia-134	121	21	0.4	0.4	NUM
biotropia-134	121	22	cm	cm	NOUN
biotropia-134	121	23	cuvette	cuvette	NOUN
biotropia-134	121	24	(	(	PUNCT
biotropia-134	121	25	0	0	NUM
biotropia-134	121	26	-	-	SYM
biotropia-134	121	27	6	6	NUM
biotropia-134	121	28	%	%	NOUN
biotropia-134	121	29	)	)	PUNCT
biotropia-134	121	30	.	.	PUNCT
biotropia-134	122	1	thus	thus	ADV
biotropia-134	122	2	,	,	PUNCT
biotropia-134	122	3	droop	droop	NOUN
biotropia-134	122	4	seems	seem	VERB
biotropia-134	122	5	to	to	PART
biotropia-134	122	6	be	be	AUX
biotropia-134	122	7	affected	affect	VERB
biotropia-134	122	8	by	by	ADP
biotropia-134	122	9	the	the	DET
biotropia-134	122	10	size	size	NOUN
biotropia-134	122	11	of	of	ADP
biotropia-134	122	12	cuvette	cuvette	NOUN
biotropia-134	122	13	used	use	VERB
biotropia-134	122	14	,	,	PUNCT
biotropia-134	122	15	although	although	SCONJ
biotropia-134	122	16	no	no	DET
biotropia-134	122	17	effect	effect	NOUN
biotropia-134	122	18	on	on	ADP
biotropia-134	122	19	electroporated	electroporate	VERB
biotropia-134	122	20	sperm	sperm	NOUN
biotropia-134	122	21	motility	motility	NOUN
biotropia-134	122	22	(	(	PUNCT
biotropia-134	122	23	table	table	NOUN
biotropia-134	122	24	1	1	NUM
biotropia-134	122	25	)	)	PUNCT
biotropia-134	122	26	.	.	PUNCT
biotropia-134	123	1	droop	droop	NOUN
biotropia-134	123	2	electroporated	electroporate	VERB
biotropia-134	123	3	spermatozoa	spermatozoon	NOUN
biotropia-134	123	4	using	use	VERB
biotropia-134	123	5	dna	dna	PROPN
biotropia-134	123	6	concentration	concentration	NOUN
biotropia-134	123	7	of	of	ADP
biotropia-134	123	8	5	5	NUM
biotropia-134	123	9	μg	μg	NOUN
biotropia-134	123	10	/	/	SYM
biotropia-134	123	11	ml	ml	PROPN
biotropia-134	123	12	was	be	AUX
biotropia-134	123	13	slightly	slightly	ADV
biotropia-134	123	14	lower	low	ADJ
biotropia-134	123	15	(	(	PUNCT
biotropia-134	123	16	90	90	NUM
biotropia-134	123	17	%	%	NOUN
biotropia-134	123	18	)	)	PUNCT
biotropia-134	123	19	compared	compare	VERB
biotropia-134	123	20	to	to	ADP
biotropia-134	123	21	that	that	PRON
biotropia-134	123	22	of	of	ADP
biotropia-134	123	23	the	the	DET
biotropia-134	123	24	two	two	NUM
biotropia-134	123	25	other	other	ADJ
biotropia-134	123	26	treatments	treatment	NOUN
biotropia-134	123	27	(	(	PUNCT
biotropia-134	123	28	100	100	NUM
biotropia-134	123	29	%	%	NOUN
biotropia-134	123	30	)	)	PUNCT
biotropia-134	123	31	.	.	PUNCT
biotropia-134	124	1	sperm	sperm	NOUN
biotropia-134	124	2	in	in	ADP
biotropia-134	124	3	5	5	NUM
biotropia-134	124	4	μg/	μg/	NOUN
biotropia-134	124	5	19	19	NUM
biotropia-134	124	6	is	be	AUX
biotropia-134	124	7	a	a	DET
biotropia-134	124	8	function	function	NOUN
biotropia-134	124	9	of	of	ADP
biotropia-134	124	10	voltage	voltage	NOUN
biotropia-134	124	11	reduction	reduction	NOUN
biotropia-134	124	12	at	at	ADP
biotropia-134	124	13	the	the	DET
biotropia-134	124	14	end	end	NOUN
biotropia-134	124	15	of	of	ADP
biotropia-134	124	16	electric	electric	ADJ
biotropia-134	124	17	shock	shock	NOUN
biotropia-134	124	18	(	(	PUNCT
biotropia-134	124	19	v	v	NOUN
biotropia-134	124	20	v	v	NOUN
biotropia-134	124	21	)	)	PUNCT
biotropia-134	124	22	from	from	ADP
biotropia-134	124	23	a	a	DET
biotropia-134	124	24	starting	starting	NOUN
biotropia-134	124	25	voltage	voltage	NOUN
biotropia-134	124	26	v	v	NOUN
biotropia-134	124	27	(	(	PUNCT
biotropia-134	124	28	anonymous	anonymous	ADJ
biotropia-134	124	29	2006	2006	NUM
biotropia-134	124	30	)	)	PUNCT
biotropia-134	124	31	.	.	PUNCT
biotropia-134	125	1	0	0	NUM
biotropia-134	126	1	t	t	NOUN
biotropia-134	126	2	0	0	NUM
biotropia-134	126	3	table	table	NOUN
biotropia-134	126	4	3	3	NUM
biotropia-134	126	5	.	.	PUNCT
biotropia-134	126	6	actual	actual	ADJ
biotropia-134	126	7	voltage	voltage	NOUN
biotropia-134	126	8	,	,	PUNCT
biotropia-134	126	9	droop	droop	NOUN
biotropia-134	126	10	and	and	CCONJ
biotropia-134	126	11	percentage	percentage	NOUN
biotropia-134	126	12	of	of	ADP
biotropia-134	126	13	motile	motile	ADJ
biotropia-134	126	14	sperm	sperm	NOUN
biotropia-134	126	15	in	in	ADP
biotropia-134	126	16	electroporation	electroporation	NOUN
biotropia-134	126	17	using	use	VERB
biotropia-134	126	18	different	different	ADJ
biotropia-134	126	19	dna	dna	PROPN
biotropia-134	126	20	concentration	concentration	NOUN
biotropia-134	126	21	and	and	CCONJ
biotropia-134	126	22	cuvette	cuvette	NOUN
biotropia-134	126	23	gap	gap	NOUN
biotropia-134	126	24	size	size	NOUN
biotropia-134	126	25	dna	dna	PROPN
biotropia-134	126	26	concentration	concentration	NOUN
biotropia-134	126	27	(	(	PUNCT
biotropia-134	126	28	μg	μg	NOUN
biotropia-134	126	29	/	/	SYM
biotropia-134	126	30	ml	ml	NOUN
biotropia-134	126	31	)	)	PUNCT
biotropia-134	126	32	repetition	repetition	NOUN
biotropia-134	126	33	setting	set	VERB
biotropia-134	126	34	voltage	voltage	NOUN
biotropia-134	126	35	(	(	PUNCT
biotropia-134	126	36	volt	volt	NOUN
biotropia-134	126	37	)	)	PUNCT
biotropia-134	126	38	cuvette	cuvette	NOUN
biotropia-134	126	39	gap	gap	NOUN
biotropia-134	126	40	(	(	PUNCT
biotropia-134	126	41	cm	cm	NOUN
biotropia-134	126	42	)	)	PUNCT
biotropia-134	126	43	actual	actual	ADJ
biotropia-134	126	44	voltage	voltage	NOUN
biotropia-134	126	45	(	(	PUNCT
biotropia-134	126	46	volt	volt	NOUN
biotropia-134	126	47	)	)	PUNCT
biotropia-134	126	48	droop	droop	NOUN
biotropia-134	126	49	(	(	PUNCT
biotropia-134	126	50	%	%	INTJ
biotropia-134	126	51	)	)	PUNCT
biotropia-134	126	52	sperm	sperm	NOUN
biotropia-134	126	53	motility	motility	NOUN
biotropia-134	126	54	(	(	PUNCT
biotropia-134	126	55	%	%	NOUN
biotropia-134	126	56	)	)	PUNCT
biotropia-134	126	57	1	1	NUM
biotropia-134	126	58	50	50	NUM
biotropia-134	126	59	0.4	0.4	NUM
biotropia-134	126	60	37	37	NUM
biotropia-134	126	61	6	6	NUM
biotropia-134	126	62	90	90	NUM
biotropia-134	126	63	5	5	NUM
biotropia-134	126	64	2	2	NUM
biotropia-134	126	65	50	50	NUM
biotropia-134	126	66	0.4	0.4	NUM
biotropia-134	126	67	37	37	NUM
biotropia-134	126	68	0	0	NUM
biotropia-134	126	69	90	90	NUM
biotropia-134	126	70	3	3	NUM
biotropia-134	126	71	50	50	NUM
biotropia-134	126	72	0.2	0.2	NUM
biotropia-134	126	73	37	37	NUM
biotropia-134	126	74	12	12	NUM
biotropia-134	126	75	90	90	NUM
biotropia-134	126	76	1	1	NUM
biotropia-134	126	77	50	50	NUM
biotropia-134	126	78	0.4	0.4	NUM
biotropia-134	126	79	37	37	NUM
biotropia-134	126	80	6	6	NUM
biotropia-134	126	81	100	100	NUM
biotropia-134	126	82	10	10	NUM
biotropia-134	126	83	2	2	NUM
biotropia-134	126	84	50	50	NUM
biotropia-134	126	85	0.4	0.4	NUM
biotropia-134	126	86	37	37	NUM
biotropia-134	126	87	6	6	NUM
biotropia-134	126	88	100	100	NUM
biotropia-134	126	89	3	3	NUM
biotropia-134	126	90	50	50	NUM
biotropia-134	126	91	0.4	0.4	NUM
biotropia-134	126	92	37	37	NUM
biotropia-134	126	93	0	0	NUM
biotropia-134	126	94	100	100	NUM
biotropia-134	126	95	1	1	NUM
biotropia-134	126	96	50	50	NUM
biotropia-134	126	97	0.2	0.2	NUM
biotropia-134	126	98	37	37	NUM
biotropia-134	126	99	12	12	NUM
biotropia-134	126	100	100	100	NUM
biotropia-134	126	101	20	20	NUM
biotropia-134	126	102	2	2	NUM
biotropia-134	126	103	50	50	NUM
biotropia-134	126	104	0.2	0.2	NUM
biotropia-134	126	105	37	37	NUM
biotropia-134	126	106	12	12	NUM
biotropia-134	126	107	100	100	NUM
biotropia-134	126	108	3	3	NUM
biotropia-134	126	109	50	50	NUM
biotropia-134	126	110	0.2	0.2	NUM
biotropia-134	126	111	37	37	NUM
biotropia-134	126	112	6	6	NUM
biotropia-134	126	113	100	100	NUM
biotropia-134	126	114	m	m	NUM
biotropia-134	126	115	1	1	NUM
biotropia-134	126	116	2	2	NUM
biotropia-134	126	117	3	3	NUM
biotropia-134	126	118	4	4	NUM
biotropia-134	126	119	5	5	NUM
biotropia-134	126	120	6	6	NUM
biotropia-134	126	121	7	7	NUM
biotropia-134	126	122	8	8	NUM
biotropia-134	126	123	kb	kb	PROPN
biotropia-134	126	124	10,0	10,0	NUM
biotropia-134	126	125	3,0	3,0	NUM
biotropia-134	126	126	1,0	1,0	NUM
biotropia-134	126	127	0,7	0,7	NUM
biotropia-134	126	128	0,5	0,5	NUM
biotropia-134	126	129	0,3	0,3	NUM
biotropia-134	126	130	0,1	0,1	NUM
biotropia-134	126	131	figure	figure	NOUN
biotropia-134	126	132	3	3	NUM
biotropia-134	126	133	.	.	PUNCT
biotropia-134	126	134	pcr	pcr	ADJ
biotropia-134	126	135	amplification	amplification	NOUN
biotropia-134	126	136	product	product	NOUN
biotropia-134	126	137	of	of	ADP
biotropia-134	126	138	dna	dna	PROPN
biotropia-134	126	139	extracted	extract	VERB
biotropia-134	126	140	from	from	ADP
biotropia-134	126	141	microinjected	microinjected	ADJ
biotropia-134	126	142	embryos	embryo	NOUN
biotropia-134	126	143	at	at	ADP
biotropia-134	126	144	14	14	NUM
biotropia-134	126	145	hours	hour	NOUN
biotropia-134	126	146	post	post	NOUN
biotropia-134	126	147	injection	injection	NOUN
biotropia-134	126	148	.	.	PUNCT
biotropia-134	127	1	lanes	lane	NOUN
biotropia-134	127	2	1	1	NUM
biotropia-134	127	3	-	-	SYM
biotropia-134	127	4	2	2	NUM
biotropia-134	127	5	:	:	SYM
biotropia-134	127	6	1	1	NUM
biotropia-134	127	7	second	second	ADJ
biotropia-134	127	8	injection	injection	NOUN
biotropia-134	127	9	,	,	PUNCT
biotropia-134	127	10	lane	lane	NOUN
biotropia-134	127	11	3	3	NUM
biotropia-134	127	12	-	-	SYM
biotropia-134	127	13	4	4	NUM
biotropia-134	127	14	:	:	SYM
biotropia-134	127	15	2	2	NUM
biotropia-134	127	16	seconds	second	NOUN
biotropia-134	127	17	injection	injection	NOUN
biotropia-134	127	18	,	,	PUNCT
biotropia-134	127	19	lane	lane	NOUN
biotropia-134	127	20	5	5	NUM
biotropia-134	127	21	-	-	SYM
biotropia-134	127	22	6	6	NUM
biotropia-134	127	23	:	:	SYM
biotropia-134	127	24	3	3	NUM
biotropia-134	127	25	seconds	second	NOUN
biotropia-134	127	26	injection	injection	NOUN
biotropia-134	127	27	,	,	PUNCT
biotropia-134	127	28	lane	lane	NOUN
biotropia-134	127	29	7	7	NUM
biotropia-134	127	30	:	:	PUNCT
biotropia-134	127	31	pcr	pcr	ADJ
biotropia-134	127	32	product	product	NOUN
biotropia-134	127	33	with	with	ADP
biotropia-134	127	34	no	no	DET
biotropia-134	127	35	dna	dna	NOUN
biotropia-134	127	36	template	template	NOUN
biotropia-134	127	37	,	,	PUNCT
biotropia-134	127	38	and	and	CCONJ
biotropia-134	127	39	lane	lane	NOUN
biotropia-134	127	40	8	8	NUM
biotropia-134	127	41	is	be	AUX
biotropia-134	127	42	pcr	pcr	ADJ
biotropia-134	127	43	product	product	NOUN
biotropia-134	127	44	with	with	ADP
biotropia-134	127	45	plasmid	plasmid	ADJ
biotropia-134	127	46	pktbp	pktbp	NOUN
biotropia-134	127	47	-	-	PUNCT
biotropia-134	127	48	ktgh	ktgh	NOUN
biotropia-134	127	49	as	as	ADP
biotropia-134	127	50	template	template	NOUN
biotropia-134	127	51	.	.	PUNCT
biotropia-134	128	1	m	m	PROPN
biotropia-134	128	2	is	be	AUX
biotropia-134	128	3	2	2	NUM
biotropia-134	128	4	-	-	PUNCT
biotropia-134	128	5	log	log	NOUN
biotropia-134	128	6	ladder	ladder	NOUN
biotropia-134	128	7	dna	dna	PROPN
biotropia-134	128	8	marker	marker	NOUN
biotropia-134	128	9	(	(	PUNCT
biotropia-134	128	10	biolabs	biolabs	PROPN
biotropia-134	128	11	,	,	PUNCT
biotropia-134	128	12	inc	inc	PROPN
biotropia-134	128	13	.	.	PROPN
biotropia-134	128	14	,	,	PUNCT
biotropia-134	128	15	new	new	PROPN
biotropia-134	128	16	england	england	PROPN
biotropia-134	128	17	)	)	PUNCT
biotropia-134	128	18	.	.	PUNCT
biotropia-134	129	1	pcr	pcr	ADJ
biotropia-134	129	2	analysis	analysis	NOUN
biotropia-134	129	3	showed	show	VERB
biotropia-134	129	4	that	that	SCONJ
biotropia-134	129	5	electroporated	electroporate	VERB
biotropia-134	129	6	sperm	sperm	NOUN
biotropia-134	129	7	in	in	ADP
biotropia-134	129	8	all	all	DET
biotropia-134	129	9	treatments	treatment	NOUN
biotropia-134	129	10	contained	contain	VERB
biotropia-134	129	11	foreign	foreign	ADJ
biotropia-134	129	12	dna	dna	NOUN
biotropia-134	129	13	(	(	PUNCT
biotropia-134	129	14	fig	fig	NOUN
biotropia-134	129	15	.	.	PUNCT
biotropia-134	129	16	4	4	NUM
biotropia-134	129	17	)	)	PUNCT
biotropia-134	129	18	.	.	PUNCT
biotropia-134	130	1	thus	thus	ADV
biotropia-134	130	2	,	,	PUNCT
biotropia-134	130	3	parameters	parameter	NOUN
biotropia-134	130	4	of	of	ADP
biotropia-134	130	5	electroporation	electroporation	NOUN
biotropia-134	130	6	used	use	VERB
biotropia-134	130	7	in	in	ADP
biotropia-134	130	8	this	this	DET
biotropia-134	130	9	study	study	NOUN
biotropia-134	130	10	could	could	AUX
biotropia-134	130	11	deliver	deliver	VERB
biotropia-134	130	12	foreign	foreign	ADJ
biotropia-134	130	13	dna	dna	NOUN
biotropia-134	130	14	to	to	PART
biotropia-134	130	15	enter	enter	VERB
biotropia-134	130	16	spermatozoa	spermatozoon	NOUN
biotropia-134	130	17	.	.	PUNCT
biotropia-134	131	1	in	in	ADP
biotropia-134	131	2	addition	addition	NOUN
biotropia-134	131	3	,	,	PUNCT
biotropia-134	131	4	semi	semi	ADJ
biotropia-134	131	5	-	-	ADJ
biotropia-134	131	6	quantitative	quantitative	ADJ
biotropia-134	131	7	pcr	pcr	NOUN
biotropia-134	131	8	was	be	AUX
biotropia-134	131	9	applied	apply	VERB
biotropia-134	131	10	to	to	PART
biotropia-134	131	11	determine	determine	VERB
biotropia-134	131	12	whether	whether	SCONJ
biotropia-134	131	13	increase	increase	NOUN
biotropia-134	131	14	of	of	ADP
biotropia-134	131	15	dna	dna	PROPN
biotropia-134	131	16	concentration	concentration	NOUN
biotropia-134	131	17	used	use	VERB
biotropia-134	131	18	in	in	ADP
biotropia-134	131	19	electroporation	electroporation	NOUN
biotropia-134	131	20	could	could	AUX
biotropia-134	131	21	improve	improve	VERB
biotropia-134	131	22	number	number	NOUN
biotropia-134	131	23	of	of	ADP
biotropia-134	131	24	sperm	sperm	NOUN
biotropia-134	131	25	carrying	carry	VERB
biotropia-134	131	26	foreign	foreign	ADJ
biotropia-134	131	27	gene	gene	NOUN
biotropia-134	131	28	.	.	PUNCT
biotropia-134	132	1	as	as	SCONJ
biotropia-134	132	2	shown	show	VERB
biotropia-134	132	3	in	in	ADP
biotropia-134	132	4	figure	figure	NOUN
biotropia-134	132	5	4	4	NUM
biotropia-134	132	6	,	,	PUNCT
biotropia-134	132	7	the	the	DET
biotropia-134	132	8	thickness	thickness	NOUN
biotropia-134	132	9	of	of	ADP
biotropia-134	132	10	dna	dna	PROPN
biotropia-134	132	11	band	band	NOUN
biotropia-134	132	12	increased	increase	VERB
biotropia-134	132	13	by	by	ADP
biotropia-134	132	14	increasing	increase	VERB
biotropia-134	132	15	concentration	concentration	NOUN
biotropia-134	132	16	of	of	ADP
biotropia-134	132	17	foreign	foreign	ADJ
biotropia-134	132	18	dna	dna	PROPN
biotropia-134	132	19	used	use	VERB
biotropia-134	132	20	in	in	ADP
biotropia-134	132	21	this	this	DET
biotropia-134	132	22	electroporation	electroporation	NOUN
biotropia-134	132	23	method	method	NOUN
biotropia-134	132	24	.	.	PUNCT
biotropia-134	133	1	volume	volume	NOUN
biotropia-134	133	2	of	of	ADP
biotropia-134	133	3	semen	semen	NOUN
biotropia-134	133	4	(	(	PUNCT
biotropia-134	133	5	25	25	NUM
biotropia-134	133	6	μl	μl	ADJ
biotropia-134	133	7	,	,	PUNCT
biotropia-134	133	8	equal	equal	ADJ
biotropia-134	133	9	to	to	ADP
biotropia-134	133	10	about	about	ADP
biotropia-134	133	11	175	175	NUM
biotropia-134	133	12	million	million	NUM
biotropia-134	133	13	spermatozoa	spermatozoon	NOUN
biotropia-134	133	14	)	)	PUNCT
biotropia-134	133	15	utilized	utilize	VERB
biotropia-134	133	16	in	in	ADP
biotropia-134	133	17	electroporation	electroporation	NOUN
biotropia-134	133	18	and	and	CCONJ
biotropia-134	133	19	concentrations	concentration	NOUN
biotropia-134	133	20	of	of	ADP
biotropia-134	133	21	the	the	DET
biotropia-134	133	22	extracted	extract	VERB
biotropia-134	133	23	gene	gene	NOUN
biotropia-134	133	24	transfer	transfer	NOUN
biotropia-134	133	25	method	method	NOUN
biotropia-134	133	26	in	in	ADP
biotropia-134	133	27	humpback	humpback	NOUN
biotropia-134	133	28	grouper	grouper	NOUN
biotropia-134	133	29	alimuddin	alimuddin	PROPN
biotropia-134	133	30	.et	.et	PUNCT
biotropia-134	134	1	al	al	PROPN
biotropia-134	134	2	biotropia	biotropia	ADJ
biotropia-134	134	3	vol	vol	NOUN
biotropia-134	134	4	.	.	PROPN
biotropia-134	135	1	18	18	NUM
biotropia-134	135	2	no	no	NOUN
biotropia-134	135	3	.	.	NOUN
biotropia-134	135	4	1	1	NUM
biotropia-134	135	5	,	,	PUNCT
biotropia-134	135	6	2011	2011	NUM
biotropia-134	135	7	20	20	NUM
biotropia-134	135	8	dna	dna	NOUN
biotropia-134	135	9	from	from	ADP
biotropia-134	135	10	electroporated	electroporate	VERB
biotropia-134	135	11	spermatozoa	spermatozoon	NOUN
biotropia-134	135	12	used	use	VERB
biotropia-134	135	13	as	as	ADP
biotropia-134	135	14	template	template	NOUN
biotropia-134	135	15	in	in	ADP
biotropia-134	135	16	pcr	pcr	ADJ
biotropia-134	135	17	amplification	amplification	NOUN
biotropia-134	135	18	were	be	AUX
biotropia-134	135	19	similar	similar	ADJ
biotropia-134	135	20	among	among	ADP
biotropia-134	135	21	treatments	treatment	NOUN
biotropia-134	135	22	.	.	PUNCT
biotropia-134	136	1	thus	thus	ADV
biotropia-134	136	2	,	,	PUNCT
biotropia-134	136	3	most	most	ADV
biotropia-134	136	4	likely	likely	ADV
biotropia-134	136	5	increasing	increase	VERB
biotropia-134	136	6	of	of	ADP
biotropia-134	136	7	dna	dna	PROPN
biotropia-134	136	8	concentra	concentra	PROPN
biotropia-134	136	9	-	-	NOUN
biotropia-134	136	10	tion	tion	NOUN
biotropia-134	136	11	in	in	ADP
biotropia-134	136	12	this	this	DET
biotropia-134	136	13	study	study	NOUN
biotropia-134	136	14	increased	increase	VERB
biotropia-134	136	15	copy	copy	NOUN
biotropia-134	136	16	number	number	NOUN
biotropia-134	136	17	of	of	ADP
biotropia-134	136	18	foreign	foreign	ADJ
biotropia-134	136	19	dna	dna	NOUN
biotropia-134	136	20	in	in	ADP
biotropia-134	136	21	spermatozoa	spermatozoon	NOUN
biotropia-134	136	22	.	.	PUNCT
biotropia-134	137	1	furthermore	furthermore	ADV
biotropia-134	137	2	,	,	PUNCT
biotropia-134	137	3	higher	high	ADJ
biotropia-134	137	4	copy	copy	VERB
biotropia-134	137	5	number	number	NOUN
biotropia-134	137	6	of	of	ADP
biotropia-134	137	7	foreign	foreign	ADJ
biotropia-134	137	8	gene	gene	NOUN
biotropia-134	137	9	entering	enter	VERB
biotropia-134	137	10	spermatozoa	spermatozoon	NOUN
biotropia-134	137	11	may	may	AUX
biotropia-134	137	12	raise	raise	VERB
biotropia-134	137	13	the	the	DET
biotropia-134	137	14	possibility	possibility	NOUN
biotropia-134	137	15	of	of	ADP
biotropia-134	137	16	foreign	foreign	ADJ
biotropia-134	137	17	gene	gene	NOUN
biotropia-134	137	18	to	to	PART
biotropia-134	137	19	integrate	integrate	VERB
biotropia-134	137	20	to	to	ADP
biotropia-134	137	21	the	the	DET
biotropia-134	137	22	host	host	NOUN
biotropia-134	137	23	genome	genome	NOUN
biotropia-134	137	24	.	.	PUNCT
biotropia-134	138	1	through	through	ADP
biotropia-134	138	2	fertilization	fertilization	NOUN
biotropia-134	138	3	,	,	PUNCT
biotropia-134	138	4	those	those	DET
biotropia-134	138	5	spermatozoa	spermatozoon	NOUN
biotropia-134	138	6	would	would	AUX
biotropia-134	138	7	then	then	ADV
biotropia-134	138	8	contribute	contribute	VERB
biotropia-134	138	9	to	to	PART
biotropia-134	138	10	enhance	enhance	VERB
biotropia-134	138	11	the	the	DET
biotropia-134	138	12	number	number	NOUN
biotropia-134	138	13	of	of	ADP
biotropia-134	138	14	embryo	embryo	NOUN
biotropia-134	138	15	carrying	carry	VERB
biotropia-134	138	16	foreign	foreign	ADJ
biotropia-134	138	17	gene	gene	NOUN
biotropia-134	138	18	.	.	PUNCT
biotropia-134	139	1	n	n	PRON
biotropia-134	139	2	1	1	NUM
biotropia-134	139	3	m	m	NOUN
biotropia-134	139	4	kb	kb	PROPN
biotropia-134	139	5	3.0	3.0	NUM
biotropia-134	139	6	1.0	1.0	NUM
biotropia-134	139	7	0.5	0.5	NUM
biotropia-134	139	8	2	2	NUM
biotropia-134	139	9	3	3	NUM
biotropia-134	139	10	4	4	NUM
biotropia-134	139	11	5	5	NUM
biotropia-134	139	12	6	6	NUM
biotropia-134	139	13	7	7	NUM
biotropia-134	139	14	8	8	NUM
biotropia-134	139	15	9	9	NUM
biotropia-134	139	16	p	p	NOUN
biotropia-134	139	17	figure	figure	NOUN
biotropia-134	139	18	4	4	NUM
biotropia-134	139	19	.	.	PUNCT
biotropia-134	139	20	pcr	pcr	ADJ
biotropia-134	139	21	amplification	amplification	NOUN
biotropia-134	139	22	product	product	NOUN
biotropia-134	139	23	of	of	ADP
biotropia-134	139	24	dna	dna	PROPN
biotropia-134	139	25	extracted	extract	VERB
biotropia-134	139	26	from	from	ADP
biotropia-134	139	27	electroporated	electroporate	VERB
biotropia-134	139	28	sperm	sperm	NOUN
biotropia-134	139	29	.	.	PUNCT
biotropia-134	140	1	lanes	lane	NOUN
biotropia-134	140	2	1	1	NUM
biotropia-134	140	3	-	-	SYM
biotropia-134	140	4	3	3	NUM
biotropia-134	140	5	:	:	SYM
biotropia-134	140	6	20	20	NUM
biotropia-134	140	7	μg	μg	NOUN
biotropia-134	140	8	/	/	SYM
biotropia-134	140	9	ml	ml	X
biotropia-134	140	10	plasmid	plasmid	ADJ
biotropia-134	140	11	dna	dna	PROPN
biotropia-134	140	12	concentration	concentration	NOUN
biotropia-134	140	13	,	,	PUNCT
biotropia-134	140	14	lanes	lane	VERB
biotropia-134	140	15	4	4	NUM
biotropia-134	140	16	-	-	SYM
biotropia-134	140	17	6	6	NUM
biotropia-134	140	18	:	:	SYM
biotropia-134	140	19	10	10	NUM
biotropia-134	140	20	μg	μg	NOUN
biotropia-134	140	21	/	/	SYM
biotropia-134	140	22	ml	ml	X
biotropia-134	140	23	plasmid	plasmid	ADJ
biotropia-134	140	24	dna	dna	PROPN
biotropia-134	140	25	concentration	concentration	NOUN
biotropia-134	140	26	,	,	PUNCT
biotropia-134	140	27	lanes	lane	VERB
biotropia-134	140	28	7	7	NUM
biotropia-134	140	29	-	-	SYM
biotropia-134	140	30	9	9	NUM
biotropia-134	140	31	:	:	SYM
biotropia-134	140	32	5	5	NUM
biotropia-134	140	33	μg	μg	NOUN
biotropia-134	140	34	/	/	SYM
biotropia-134	140	35	ml	ml	X
biotropia-134	140	36	plasmid	plasmid	ADJ
biotropia-134	140	37	dna	dna	PROPN
biotropia-134	140	38	concentration	concentration	NOUN
biotropia-134	140	39	.	.	PUNCT
biotropia-134	141	1	m	m	PROPN
biotropia-134	141	2	is	be	AUX
biotropia-134	141	3	2	2	NUM
biotropia-134	141	4	-	-	PUNCT
biotropia-134	141	5	log	log	NOUN
biotropia-134	141	6	ladder	ladder	NOUN
biotropia-134	141	7	dna	dna	PROPN
biotropia-134	141	8	marker	marker	NOUN
biotropia-134	141	9	(	(	PUNCT
biotropia-134	141	10	biolabs	biolabs	PROPN
biotropia-134	141	11	,	,	PUNCT
biotropia-134	141	12	inc	inc	PROPN
biotropia-134	141	13	.	.	PROPN
biotropia-134	141	14	,	,	PUNCT
biotropia-134	141	15	new	new	PROPN
biotropia-134	141	16	england	england	PROPN
biotropia-134	141	17	)	)	PUNCT
biotropia-134	141	18	.	.	PUNCT
biotropia-134	142	1	n	n	PROPN
biotropia-134	142	2	is	be	AUX
biotropia-134	142	3	pcr	pcr	ADJ
biotropia-134	142	4	product	product	NOUN
biotropia-134	142	5	without	without	ADP
biotropia-134	142	6	dna	dna	PROPN
biotropia-134	142	7	template	template	NOUN
biotropia-134	142	8	.	.	PUNCT
biotropia-134	143	1	p	p	NOUN
biotropia-134	143	2	is	be	AUX
biotropia-134	143	3	pcr	pcr	ADJ
biotropia-134	143	4	product	product	NOUN
biotropia-134	143	5	with	with	ADP
biotropia-134	143	6	plasmid	plasmid	ADJ
biotropia-134	143	7	pktbp	pktbp	NOUN
biotropia-134	143	8	-	-	PUNCT
biotropia-134	143	9	ktgh	ktgh	NOUN
biotropia-134	143	10	as	as	ADP
biotropia-134	143	11	template	template	NOUN
biotropia-134	143	12	.	.	PUNCT
biotropia-134	144	1	efficacy	efficacy	NOUN
biotropia-134	144	2	of	of	ADP
biotropia-134	144	3	sperm	sperm	NOUN
biotropia-134	144	4	-	-	PUNCT
biotropia-134	144	5	mediated	mediate	VERB
biotropia-134	144	6	gene	gene	NOUN
biotropia-134	144	7	transfer	transfer	NOUN
biotropia-134	144	8	(	(	PUNCT
biotropia-134	144	9	smgt	smgt	NOUN
biotropia-134	144	10	)	)	PUNCT
biotropia-134	144	11	technique	technique	NOUN
biotropia-134	144	12	to	to	PART
biotropia-134	144	13	produce	produce	VERB
biotropia-134	144	14	a	a	DET
biotropia-134	144	15	transgenic	transgenic	NOUN
biotropia-134	144	16	marine	marine	ADJ
biotropia-134	144	17	species	specie	NOUN
biotropia-134	144	18	has	have	AUX
biotropia-134	144	19	been	be	AUX
biotropia-134	144	20	proved	prove	VERB
biotropia-134	144	21	by	by	ADP
biotropia-134	144	22	lu	lu	PROPN
biotropia-134	144	23	.	.	PUNCT
biotropia-134	145	1	(	(	PUNCT
biotropia-134	145	2	2002	2002	NUM
biotropia-134	145	3	)	)	PUNCT
biotropia-134	145	4	and	and	CCONJ
biotropia-134	145	5	sun	sun	NOUN
biotropia-134	145	6	(	(	PUNCT
biotropia-134	145	7	2004	2004	NUM
biotropia-134	145	8	)	)	PUNCT
biotropia-134	145	9	.	.	PUNCT
biotropia-134	146	1	smgt	smgt	PROPN
biotropia-134	146	2	method	method	NOUN
biotropia-134	146	3	has	have	AUX
biotropia-134	146	4	also	also	ADV
biotropia-134	146	5	been	be	AUX
biotropia-134	146	6	applied	apply	VERB
biotropia-134	146	7	to	to	PART
biotropia-134	146	8	introduce	introduce	VERB
biotropia-134	146	9	foreign	foreign	ADJ
biotropia-134	146	10	gene	gene	NOUN
biotropia-134	146	11	into	into	ADP
biotropia-134	146	12	humpback	humpback	NOUN
biotropia-134	146	13	grouper	grouper	NOUN
biotropia-134	146	14	sperm	sperm	NOUN
biotropia-134	146	15	.	.	PUNCT
biotropia-134	147	1	three	three	NUM
biotropia-134	147	2	smgt	smgt	ADJ
biotropia-134	147	3	methods	method	NOUN
biotropia-134	147	4	(	(	PUNCT
biotropia-134	147	5	electroporation	electroporation	NOUN
biotropia-134	147	6	,	,	PUNCT
biotropia-134	147	7	and	and	CCONJ
biotropia-134	147	8	transfection	transfection	NOUN
biotropia-134	147	9	)	)	PUNCT
biotropia-134	147	10	were	be	AUX
biotropia-134	147	11	examined	examine	VERB
biotropia-134	147	12	in	in	ADP
biotropia-134	147	13	this	this	DET
biotropia-134	147	14	study	study	NOUN
biotropia-134	147	15	.	.	PUNCT
biotropia-134	148	1	transfection	transfection	NOUN
biotropia-134	148	2	using	use	VERB
biotropia-134	148	3	jetpei	jetpei	PROPN
biotropia-134	148	4	reagent	reagent	NOUN
biotropia-134	148	5	succeeds	succeed	VERB
biotropia-134	148	6	to	to	PART
biotropia-134	148	7	be	be	AUX
biotropia-134	148	8	used	use	VERB
biotropia-134	148	9	in	in	ADP
biotropia-134	148	10	white	white	PROPN
biotropia-134	148	11	shrimp	shrimp	PROPN
biotropia-134	148	12	(	(	PUNCT
biotropia-134	148	13	sun	sun	NOUN
biotropia-134	148	14	.	.	PUNCT
biotropia-134	149	1	2005	2005	NUM
biotropia-134	149	2	)	)	PUNCT
biotropia-134	149	3	.	.	PUNCT
biotropia-134	150	1	in	in	ADP
biotropia-134	150	2	this	this	DET
biotropia-134	150	3	study	study	NOUN
biotropia-134	150	4	,	,	PUNCT
biotropia-134	150	5	humpback	humpback	NOUN
biotropia-134	150	6	grouper	grouper	NOUN
biotropia-134	150	7	sperm	sperm	NOUN
biotropia-134	150	8	died	die	VERB
biotropia-134	150	9	when	when	SCONJ
biotropia-134	150	10	transfection	transfection	NOUN
biotropia-134	150	11	was	be	AUX
biotropia-134	150	12	performed	perform	VERB
biotropia-134	150	13	using	use	VERB
biotropia-134	150	14	jetpei	jetpei	PROPN
biotropia-134	150	15	reagent	reagent	NOUN
biotropia-134	150	16	.	.	PUNCT
biotropia-134	151	1	any	any	DET
biotropia-134	151	2	compounds	compound	NOUN
biotropia-134	151	3	of	of	ADP
biotropia-134	151	4	jetpei	jetpei	PROPN
biotropia-134	151	5	may	may	AUX
biotropia-134	151	6	be	be	AUX
biotropia-134	151	7	toxic	toxic	ADJ
biotropia-134	151	8	for	for	ADP
biotropia-134	151	9	humpback	humpback	NOUN
biotropia-134	151	10	grouper	grouper	NOUN
biotropia-134	151	11	sperm	sperm	NOUN
biotropia-134	151	12	.	.	PUNCT
biotropia-134	152	1	in	in	ADP
biotropia-134	152	2	contrast	contrast	NOUN
biotropia-134	152	3	,	,	PUNCT
biotropia-134	152	4	transfection	transfection	NOUN
biotropia-134	152	5	using	use	VERB
biotropia-134	152	6	transfast	transfast	NOUN
biotropia-134	152	7	reagent	reagent	NOUN
biotropia-134	152	8	allowed	allow	VERB
biotropia-134	152	9	humpback	humpback	NOUN
biotropia-134	152	10	grouper	grouper	NOUN
biotropia-134	152	11	sperm	sperm	NOUN
biotropia-134	152	12	to	to	PART
biotropia-134	152	13	survive	survive	VERB
biotropia-134	152	14	.	.	PUNCT
biotropia-134	153	1	transfast	transfast	NOUN
biotropia-134	153	2	transfected	transfecte	VERB
biotropia-134	153	3	-	-	PUNCT
biotropia-134	153	4	sperm	sperm	NOUN
biotropia-134	153	5	also	also	ADV
biotropia-134	153	6	carried	carry	VERB
biotropia-134	153	7	foreign	foreign	ADJ
biotropia-134	153	8	gene	gene	NOUN
biotropia-134	153	9	,	,	PUNCT
biotropia-134	153	10	although	although	SCONJ
biotropia-134	153	11	this	this	PRON
biotropia-134	153	12	was	be	AUX
biotropia-134	153	13	only	only	ADV
biotropia-134	153	14	found	find	VERB
biotropia-134	153	15	when	when	SCONJ
biotropia-134	153	16	transfection	transfection	NOUN
biotropia-134	153	17	was	be	AUX
biotropia-134	153	18	conducted	conduct	VERB
biotropia-134	153	19	for	for	ADP
biotropia-134	153	20	48	48	NUM
biotropia-134	153	21	hours	hour	NOUN
biotropia-134	153	22	at	at	ADP
biotropia-134	153	23	25	25	NUM
biotropia-134	153	24	c	c	NOUN
biotropia-134	153	25	incubation	incubation	NOUN
biotropia-134	153	26	.	.	PUNCT
biotropia-134	154	1	furthermore	furthermore	ADV
biotropia-134	154	2	,	,	PUNCT
biotropia-134	154	3	transfection	transfection	NOUN
biotropia-134	154	4	by	by	ADP
biotropia-134	154	5	injecting	inject	VERB
biotropia-134	154	6	dna	dna	NOUN
biotropia-134	154	7	-	-	PUNCT
biotropia-134	154	8	transfast	transfast	NOUN
biotropia-134	154	9	complex	complex	NOUN
biotropia-134	154	10	into	into	ADP
biotropia-134	154	11	testis	testis	NOUN
biotropia-134	154	12	could	could	AUX
biotropia-134	154	13	also	also	ADV
biotropia-134	154	14	deliver	deliver	VERB
biotropia-134	154	15	foreign	foreign	ADJ
biotropia-134	154	16	gene	gene	NOUN
biotropia-134	154	17	to	to	ADP
biotropia-134	154	18	sperm	sperm	NOUN
biotropia-134	154	19	.	.	PUNCT
biotropia-134	155	1	foreign	foreign	ADJ
biotropia-134	155	2	gene	gene	NOUN
biotropia-134	155	3	could	could	AUX
biotropia-134	155	4	be	be	AUX
biotropia-134	155	5	detected	detect	VERB
biotropia-134	155	6	in	in	ADP
biotropia-134	155	7	transfected	transfecte	VERB
biotropia-134	155	8	sperm	sperm	NOUN
biotropia-134	155	9	at	at	ADP
biotropia-134	155	10	least	least	ADV
biotropia-134	155	11	36	36	NUM
biotropia-134	155	12	hours	hour	NOUN
biotropia-134	155	13	post	post	NOUN
biotropia-134	155	14	injection	injection	NOUN
biotropia-134	155	15	.	.	PUNCT
biotropia-134	156	1	transgenic	transgenic	NOUN
biotropia-134	156	2	sea	sea	NOUN
biotropia-134	156	3	bream	bream	NOUN
biotropia-134	156	4	could	could	AUX
biotropia-134	156	5	be	be	AUX
biotropia-134	156	6	obtained	obtain	VERB
biotropia-134	156	7	by	by	ADP
biotropia-134	156	8	transfection	transfection	NOUN
biotropia-134	156	9	for	for	ADP
biotropia-134	156	10	at	at	ADV
biotropia-134	156	11	least	least	ADV
biotropia-134	156	12	48	48	NUM
biotropia-134	156	13	hous	hous	ADJ
biotropia-134	156	14	prior	prior	ADV
biotropia-134	156	15	to	to	ADP
biotropia-134	156	16	spawning	spawning	NOUN
biotropia-134	156	17	(	(	PUNCT
biotropia-134	156	18	lu	lu	PROPN
biotropia-134	156	19	.	.	PUNCT
biotropia-134	157	1	2002	2002	NUM
biotropia-134	157	2	)	)	PUNCT
biotropia-134	157	3	.	.	PUNCT
biotropia-134	158	1	in	in	ADP
biotropia-134	158	2	the	the	DET
biotropia-134	158	3	same	same	ADJ
biotropia-134	158	4	way	way	NOUN
biotropia-134	158	5	with	with	ADP
biotropia-134	158	6	sea	sea	NOUN
biotropia-134	158	7	bream	bream	NOUN
biotropia-134	158	8	,	,	PUNCT
biotropia-134	158	9	transgenic	transgenic	NOUN
biotropia-134	158	10	humpback	humpback	NOUN
biotropia-134	158	11	grouper	grouper	NOUN
biotropia-134	158	12	may	may	AUX
biotropia-134	158	13	also	also	ADV
biotropia-134	158	14	be	be	AUX
biotropia-134	158	15	produced	produce	VERB
biotropia-134	158	16	.	.	PUNCT
biotropia-134	159	1	in	in	ADP
biotropia-134	159	2	this	this	DET
biotropia-134	159	3	study	study	NOUN
biotropia-134	159	4	,	,	PUNCT
biotropia-134	159	5	however	however	ADV
biotropia-134	159	6	,	,	PUNCT
biotropia-134	159	7	only	only	ADV
biotropia-134	159	8	two	two	NUM
biotropia-134	159	9	out	out	ADP
biotropia-134	159	10	of	of	ADP
biotropia-134	159	11	ten	ten	NUM
biotropia-134	159	12	injected	inject	VERB
biotropia-134	159	13	broodstocks	broodstock	NOUN
biotropia-134	159	14	carried	carry	VERB
biotropia-134	159	15	foreign	foreign	ADJ
biotropia-134	159	16	gene	gene	NOUN
biotropia-134	159	17	in	in	ADP
biotropia-134	159	18	their	their	PRON
biotropia-134	159	19	sperm	sperm	NOUN
biotropia-134	159	20	.	.	PUNCT
biotropia-134	160	1	this	this	PRON
biotropia-134	160	2	might	might	AUX
biotropia-134	160	3	be	be	AUX
biotropia-134	160	4	due	due	ADJ
biotropia-134	160	5	to	to	ADP
biotropia-134	160	6	the	the	DET
biotropia-134	160	7	difference	difference	NOUN
biotropia-134	160	8	in	in	ADP
biotropia-134	160	9	testis	testis	NOUN
biotropia-134	160	10	maturity	maturity	NOUN
biotropia-134	160	11	.	.	PUNCT
biotropia-134	161	1	in	in	ADP
biotropia-134	161	2	addition	addition	NOUN
biotropia-134	161	3	,	,	PUNCT
biotropia-134	161	4	by	by	ADP
biotropia-134	161	5	applying	apply	VERB
biotropia-134	161	6	the	the	DET
biotropia-134	161	7	spawning	spawning	NOUN
biotropia-134	161	8	system	system	NOUN
biotropia-134	161	9	for	for	ADP
biotropia-134	161	10	humpback	humpback	NOUN
biotropia-134	161	11	grouper	grouper	NOUN
biotropia-134	161	12	,	,	PUNCT
biotropia-134	161	13	screening	screen	VERB
biotropia-134	161	14	of	of	ADP
biotropia-134	161	15	founder	founder	NOUN
biotropia-134	161	16	transgenic	transgenic	NOUN
biotropia-134	161	17	fish	fish	NOUN
biotropia-134	161	18	generated	generate	VERB
biotropia-134	161	19	by	by	ADP
biotropia-134	161	20	transfection	transfection	NOUN
biotropia-134	161	21	method	method	NOUN
biotropia-134	161	22	is	be	AUX
biotropia-134	161	23	costly	costly	ADJ
biotropia-134	161	24	,	,	PUNCT
biotropia-134	161	25	labor	labor	NOUN
biotropia-134	161	26	-	-	PUNCT
biotropia-134	161	27	extensive	extensive	ADJ
biotropia-134	161	28	and	and	CCONJ
biotropia-134	161	29	time	time	NOUN
biotropia-134	161	30	-	-	PUNCT
biotropia-134	161	31	consuming	consume	VERB
biotropia-134	161	32	.	.	PUNCT
biotropia-134	162	1	microinjection	microinjection	NOUN
biotropia-134	162	2	is	be	AUX
biotropia-134	162	3	generally	generally	ADV
biotropia-134	162	4	applied	apply	VERB
biotropia-134	162	5	to	to	PART
biotropia-134	162	6	produce	produce	VERB
biotropia-134	162	7	transgenic	transgenic	NOUN
biotropia-134	162	8	fish	fish	NOUN
biotropia-134	162	9	.	.	PUNCT
biotropia-134	163	1	this	this	DET
biotropia-134	163	2	method	method	NOUN
biotropia-134	163	3	has	have	AUX
biotropia-134	163	4	also	also	ADV
biotropia-134	163	5	been	be	AUX
biotropia-134	163	6	used	use	VERB
biotropia-134	163	7	to	to	PART
biotropia-134	163	8	introduce	introduce	VERB
biotropia-134	163	9	foreign	foreign	ADJ
biotropia-134	163	10	gene	gene	NOUN
biotropia-134	163	11	through	through	ADP
biotropia-134	163	12	fertilized	fertilize	VERB
biotropia-134	163	13	eggs	egg	NOUN
biotropia-134	163	14	towards	towards	ADP
biotropia-134	163	15	generation	generation	NOUN
biotropia-134	163	16	of	of	ADP
biotropia-134	163	17	transgenic	transgenic	NOUN
biotropia-134	163	18	humpback	humpback	NOUN
biotropia-134	163	19	groper	groper	NOUN
biotropia-134	163	20	.	.	PUNCT
biotropia-134	164	1	as	as	SCONJ
biotropia-134	164	2	shown	show	VERB
biotropia-134	164	3	in	in	ADP
biotropia-134	164	4	figure	figure	NOUN
biotropia-134	164	5	3	3	NUM
biotropia-134	164	6	,	,	PUNCT
biotropia-134	164	7	injected	inject	VERB
biotropia-134	164	8	embryos	embryo	NOUN
biotropia-134	164	9	carried	carry	VERB
biotropia-134	164	10	the	the	DET
biotropia-134	164	11	foreign	foreign	ADJ
biotropia-134	164	12	gene	gene	NOUN
biotropia-134	164	13	at	at	ADP
biotropia-134	164	14	least	least	ADJ
biotropia-134	164	15	until	until	ADP
biotropia-134	164	16	14	14	NUM
biotropia-134	164	17	hours	hour	NOUN
biotropia-134	164	18	post	post	NOUN
biotropia-134	164	19	injection	injection	NOUN
biotropia-134	164	20	.	.	PUNCT
biotropia-134	165	1	however	however	ADV
biotropia-134	165	2	,	,	PUNCT
biotropia-134	165	3	in	in	ADP
biotropia-134	165	4	this	this	DET
biotropia-134	165	5	study	study	NOUN
biotropia-134	165	6	,	,	PUNCT
biotropia-134	165	7	none	none	NOUN
biotropia-134	165	8	or	or	CCONJ
biotropia-134	165	9	very	very	ADV
biotropia-134	165	10	low	low	ADJ
biotropia-134	165	11	number	number	NOUN
biotropia-134	165	12	of	of	ADP
biotropia-134	165	13	injected	inject	VERB
biotropia-134	165	14	embryos	embryo	NOUN
biotropia-134	165	15	hatched	hatch	VERB
biotropia-134	165	16	.	.	PUNCT
biotropia-134	166	1	as	as	SCONJ
biotropia-134	166	2	in	in	ADP
biotropia-134	166	3	other	other	ADJ
biotropia-134	166	4	marine	marine	NOUN
biotropia-134	166	5	finfish	finfish	NOUN
biotropia-134	166	6	,	,	PUNCT
biotropia-134	166	7	eggs	egg	NOUN
biotropia-134	166	8	of	of	ADP
biotropia-134	166	9	et	et	NOUN
biotropia-134	166	10	al	al	PROPN
biotropia-134	166	11	et	et	PROPN
biotropia-134	166	12	al	al	PROPN
biotropia-134	166	13	.	.	PROPN
biotropia-134	166	14	in	in	ADP
biotropia-134	166	15	vitro	vitro	X
biotropia-134	166	16	in	in	ADP
biotropia-134	166	17	vivo	vivo	NOUN
biotropia-134	166	18	in	in	ADP
biotropia-134	166	19	vitro	vitro	X
biotropia-134	166	20	et	et	PROPN
biotropia-134	166	21	al	al	PROPN
biotropia-134	166	22	in	in	ADP
biotropia-134	166	23	vitro	vitro	X
biotropia-134	166	24	in	in	ADP
biotropia-134	166	25	vivo	vivo	NOUN
biotropia-134	166	26	in	in	ADP
biotropia-134	166	27	vivo	vivo	NOUN
biotropia-134	166	28	et	et	NOUN
biotropia-134	166	29	al	al	PROPN
biotropia-134	166	30	in	in	ADP
biotropia-134	166	31	vivo	vivo	ADJ
biotropia-134	166	32	o	o	NOUN
biotropia-134	166	33	21	21	NUM
biotropia-134	166	34	humpback	humpback	NOUN
biotropia-134	166	35	grouper	grouper	NOUN
biotropia-134	166	36	float	float	NOUN
biotropia-134	166	37	in	in	ADP
biotropia-134	166	38	water	water	NOUN
biotropia-134	166	39	,	,	PUNCT
biotropia-134	166	40	small	small	ADJ
biotropia-134	166	41	in	in	ADP
biotropia-134	166	42	size	size	NOUN
biotropia-134	166	43	and	and	CCONJ
biotropia-134	166	44	has	have	VERB
biotropia-134	166	45	unclear	unclear	ADJ
biotropia-134	166	46	blastodisc	blastodisc	NOUN
biotropia-134	166	47	.	.	PUNCT
biotropia-134	167	1	these	these	DET
biotropia-134	167	2	conditions	condition	NOUN
biotropia-134	167	3	hamper	hamper	VERB
biotropia-134	167	4	microinjection	microinjection	NOUN
biotropia-134	167	5	precisely	precisely	ADV
biotropia-134	167	6	.	.	PUNCT
biotropia-134	168	1	in	in	ADP
biotropia-134	168	2	addition	addition	NOUN
biotropia-134	168	3	,	,	PUNCT
biotropia-134	168	4	the	the	DET
biotropia-134	168	5	time	time	NOUN
biotropia-134	168	6	to	to	PART
biotropia-134	168	7	reach	reach	VERB
biotropia-134	168	8	2	2	NUM
biotropia-134	168	9	-	-	PUNCT
biotropia-134	168	10	cell	cell	NOUN
biotropia-134	168	11	stage	stage	NOUN
biotropia-134	168	12	of	of	ADP
biotropia-134	168	13	humpback	humpback	NOUN
biotropia-134	168	14	grouper	grouper	NOUN
biotropia-134	168	15	embryos	embryo	NOUN
biotropia-134	168	16	is	be	AUX
biotropia-134	168	17	about	about	ADV
biotropia-134	168	18	20	20	NUM
biotropia-134	168	19	-	-	SYM
biotropia-134	168	20	30	30	NUM
biotropia-134	168	21	min	min	NOUN
biotropia-134	168	22	post	post	NOUN
biotropia-134	168	23	fertilization	fertilization	NOUN
biotropia-134	168	24	.	.	PUNCT
biotropia-134	169	1	so	so	ADV
biotropia-134	169	2	the	the	DET
biotropia-134	169	3	number	number	NOUN
biotropia-134	169	4	of	of	ADP
biotropia-134	169	5	embryos	embryo	NOUN
biotropia-134	169	6	which	which	PRON
biotropia-134	169	7	could	could	AUX
biotropia-134	169	8	be	be	AUX
biotropia-134	169	9	injected	inject	VERB
biotropia-134	169	10	by	by	ADP
biotropia-134	169	11	one	one	NUM
biotropia-134	169	12	microinjector	microinjector	NOUN
biotropia-134	169	13	is	be	AUX
biotropia-134	169	14	very	very	ADV
biotropia-134	169	15	limited	limited	ADJ
biotropia-134	169	16	(	(	PUNCT
biotropia-134	169	17	60100	60100	NUM
biotropia-134	169	18	embryos	embryo	NOUN
biotropia-134	169	19	)	)	PUNCT
biotropia-134	169	20	.	.	PUNCT
biotropia-134	170	1	hatching	hatch	VERB
biotropia-134	170	2	rate	rate	NOUN
biotropia-134	170	3	of	of	ADP
biotropia-134	170	4	uninjected	uninjected	ADJ
biotropia-134	170	5	embryos	embryo	NOUN
biotropia-134	170	6	is	be	AUX
biotropia-134	170	7	high	high	ADJ
biotropia-134	170	8	,	,	PUNCT
biotropia-134	170	9	but	but	CCONJ
biotropia-134	170	10	the	the	DET
biotropia-134	170	11	survival	survival	NOUN
biotropia-134	170	12	rate	rate	NOUN
biotropia-134	170	13	of	of	ADP
biotropia-134	170	14	larvae	larvae	NOUN
biotropia-134	170	15	is	be	AUX
biotropia-134	170	16	lower	low	ADJ
biotropia-134	170	17	i.e.	i.e.	X
biotropia-134	170	18	5	5	NUM
biotropia-134	170	19	-	-	SYM
biotropia-134	170	20	10	10	NUM
biotropia-134	170	21	%	%	NOUN
biotropia-134	170	22	in	in	ADP
biotropia-134	170	23	average	average	NOUN
biotropia-134	170	24	.	.	PUNCT
biotropia-134	171	1	based	base	VERB
biotropia-134	171	2	on	on	ADP
biotropia-134	171	3	the	the	DET
biotropia-134	171	4	results	result	NOUN
biotropia-134	171	5	in	in	ADP
biotropia-134	171	6	zebrafish	zebrafish	ADJ
biotropia-134	171	7	transgenic	transgenic	NOUN
biotropia-134	171	8	research	research	NOUN
biotropia-134	171	9	,	,	PUNCT
biotropia-134	171	10	the	the	DET
biotropia-134	171	11	number	number	NOUN
biotropia-134	171	12	of	of	ADP
biotropia-134	171	13	germline	germline	NOUN
biotropia-134	171	14	transgenic	transgenic	NOUN
biotropia-134	171	15	f0	f0	PROPN
biotropia-134	171	16	is	be	AUX
biotropia-134	171	17	2	2	NUM
biotropia-134	171	18	-	-	SYM
biotropia-134	171	19	4	4	NUM
biotropia-134	171	20	%	%	NOUN
biotropia-134	171	21	of	of	ADP
biotropia-134	171	22	survived	survive	VERB
biotropia-134	171	23	fish	fish	NOUN
biotropia-134	171	24	(	(	PUNCT
biotropia-134	171	25	alimuddin	alimuddin	NOUN
biotropia-134	171	26	.	.	PUNCT
biotropia-134	172	1	2005	2005	NUM
biotropia-134	172	2	;	;	PUNCT
biotropia-134	172	3	alimuddin	alimuddin	VERB
biotropia-134	172	4	.	.	PUNCT
biotropia-134	173	1	2008	2008	NUM
biotropia-134	173	2	)	)	PUNCT
biotropia-134	173	3	.	.	PUNCT
biotropia-134	174	1	if	if	SCONJ
biotropia-134	174	2	we	we	PRON
biotropia-134	174	3	assume	assume	VERB
biotropia-134	174	4	that	that	SCONJ
biotropia-134	174	5	similar	similar	ADJ
biotropia-134	174	6	number	number	NOUN
biotropia-134	174	7	of	of	ADP
biotropia-134	174	8	germline	germline	NOUN
biotropia-134	174	9	transmitted	transmit	VERB
biotropia-134	174	10	f0	f0	PROPN
biotropia-134	174	11	in	in	ADP
biotropia-134	174	12	zebrafish	zebrafish	NUM
biotropia-134	174	13	can	can	AUX
biotropia-134	174	14	be	be	AUX
biotropia-134	174	15	achieved	achieve	VERB
biotropia-134	174	16	in	in	ADP
biotropia-134	174	17	humpback	humpback	NOUN
biotropia-134	174	18	grouper	grouper	NOUN
biotropia-134	174	19	,	,	PUNCT
biotropia-134	174	20	the	the	DET
biotropia-134	174	21	number	number	NOUN
biotropia-134	174	22	of	of	ADP
biotropia-134	174	23	embryos	embryo	NOUN
biotropia-134	174	24	injected	inject	VERB
biotropia-134	174	25	will	will	AUX
biotropia-134	174	26	be	be	AUX
biotropia-134	174	27	at	at	ADP
biotropia-134	174	28	least	least	ADJ
biotropia-134	174	29	1000	1000	NUM
biotropia-134	174	30	embryos	embryo	NOUN
biotropia-134	174	31	to	to	PART
biotropia-134	174	32	obtain	obtain	VERB
biotropia-134	174	33	2	2	NUM
biotropia-134	174	34	transgenic	transgenic	NOUN
biotropia-134	174	35	f0	f0	NOUN
biotropia-134	174	36	.	.	PUNCT
biotropia-134	175	1	this	this	PRON
biotropia-134	175	2	means	mean	VERB
biotropia-134	175	3	that	that	SCONJ
biotropia-134	175	4	microinjection	microinjection	NOUN
biotropia-134	175	5	should	should	AUX
biotropia-134	175	6	be	be	AUX
biotropia-134	175	7	conducted	conduct	VERB
biotropia-134	175	8	10	10	NUM
biotropia-134	175	9	times	time	NOUN
biotropia-134	175	10	,	,	PUNCT
biotropia-134	175	11	or	or	CCONJ
biotropia-134	175	12	we	we	PRON
biotropia-134	175	13	need	need	VERB
biotropia-134	175	14	more	more	ADJ
biotropia-134	175	15	microinjectors	microinjector	NOUN
biotropia-134	175	16	and	and	CCONJ
biotropia-134	175	17	technicians	technician	NOUN
biotropia-134	175	18	.	.	PUNCT
biotropia-134	176	1	thus	thus	ADV
biotropia-134	176	2	,	,	PUNCT
biotropia-134	176	3	the	the	DET
biotropia-134	176	4	use	use	NOUN
biotropia-134	176	5	of	of	ADP
biotropia-134	176	6	microinjection	microinjection	NOUN
biotropia-134	176	7	technique	technique	NOUN
biotropia-134	176	8	to	to	PART
biotropia-134	176	9	produce	produce	VERB
biotropia-134	176	10	humpback	humpback	NOUN
biotropia-134	176	11	grouper	grouper	NOUN
biotropia-134	176	12	requires	require	VERB
biotropia-134	176	13	facilities	facility	NOUN
biotropia-134	176	14	,	,	PUNCT
biotropia-134	176	15	and	and	CCONJ
biotropia-134	176	16	is	be	AUX
biotropia-134	176	17	labor	labor	NOUN
biotropia-134	176	18	-	-	PUNCT
biotropia-134	176	19	extensive	extensive	ADJ
biotropia-134	176	20	as	as	ADV
biotropia-134	176	21	well	well	ADV
biotropia-134	176	22	as	as	ADP
biotropia-134	176	23	time	time	NOUN
biotropia-134	176	24	-	-	PUNCT
biotropia-134	176	25	consuming	consume	VERB
biotropia-134	176	26	electroporation	electroporation	NOUN
biotropia-134	176	27	has	have	AUX
biotropia-134	176	28	been	be	AUX
biotropia-134	176	29	reported	report	VERB
biotropia-134	176	30	to	to	PART
biotropia-134	176	31	be	be	AUX
biotropia-134	176	32	a	a	DET
biotropia-134	176	33	simple	simple	ADJ
biotropia-134	176	34	and	and	CCONJ
biotropia-134	176	35	mass	mass	ADJ
biotropia-134	176	36	transgenic	transgenic	NOUN
biotropia-134	176	37	production	production	NOUN
biotropia-134	176	38	method	method	NOUN
biotropia-134	176	39	.	.	PUNCT
biotropia-134	177	1	this	this	DET
biotropia-134	177	2	technique	technique	NOUN
biotropia-134	177	3	also	also	ADV
biotropia-134	177	4	has	have	VERB
biotropia-134	177	5	similar	similar	ADJ
biotropia-134	177	6	efficiency	efficiency	NOUN
biotropia-134	177	7	with	with	ADP
biotropia-134	177	8	transfection	transfection	NOUN
biotropia-134	177	9	method	method	NOUN
biotropia-134	177	10	to	to	PART
biotropia-134	177	11	produce	produce	VERB
biotropia-134	177	12	transgenic	transgenic	NOUN
biotropia-134	177	13	sea	sea	NOUN
biotropia-134	177	14	bream	bream	NOUN
biotropia-134	177	15	(	(	PUNCT
biotropia-134	177	16	lu	lu	PROPN
biotropia-134	177	17	.	.	PUNCT
biotropia-134	177	18	2002	2002	NUM
biotropia-134	177	19	)	)	PUNCT
biotropia-134	177	20	.	.	PUNCT
biotropia-134	178	1	voltage	voltage	NOUN
biotropia-134	178	2	(	(	PUNCT
biotropia-134	178	3	50	50	NUM
biotropia-134	178	4	v	v	NOUN
biotropia-134	178	5	)	)	PUNCT
biotropia-134	178	6	,	,	PUNCT
biotropia-134	178	7	pulse	pulse	NOUN
biotropia-134	178	8	length	length	NOUN
biotropia-134	178	9	(	(	PUNCT
biotropia-134	178	10	30	30	NUM
biotropia-134	178	11	μs	μs	NUM
biotropia-134	178	12	)	)	PUNCT
biotropia-134	178	13	and	and	CCONJ
biotropia-134	178	14	number	number	NOUN
biotropia-134	178	15	of	of	ADP
biotropia-134	178	16	pulse	pulse	NOUN
biotropia-134	178	17	(	(	PUNCT
biotropia-134	178	18	5	5	NUM
biotropia-134	178	19	pulses	pulse	NOUN
biotropia-134	178	20	)	)	PUNCT
biotropia-134	178	21	were	be	AUX
biotropia-134	178	22	also	also	ADV
biotropia-134	178	23	lower	low	ADJ
biotropia-134	178	24	compared	compare	VERB
biotropia-134	178	25	to	to	ADP
biotropia-134	178	26	those	those	PRON
biotropia-134	178	27	of	of	ADP
biotropia-134	178	28	sea	sea	NOUN
biotropia-134	178	29	bream	bream	NOUN
biotropia-134	178	30	.	.	PUNCT
biotropia-134	179	1	electroporation	electroporation	NOUN
biotropia-134	179	2	in	in	ADP
biotropia-134	179	3	sea	sea	NOUN
biotropia-134	179	4	bream	bream	NOUN
biotropia-134	179	5	is	be	AUX
biotropia-134	179	6	conducted	conduct	VERB
biotropia-134	179	7	using	use	VERB
biotropia-134	179	8	600	600	NUM
biotropia-134	179	9	-	-	SYM
biotropia-134	179	10	2000	2000	NUM
biotropia-134	179	11	v	v	NOUN
biotropia-134	179	12	,	,	PUNCT
biotropia-134	179	13	40	40	NUM
biotropia-134	179	14	μs	μs	NUM
biotropia-134	179	15	pulse	pulse	NOUN
biotropia-134	179	16	length	length	NOUN
biotropia-134	179	17	,	,	PUNCT
biotropia-134	179	18	and	and	CCONJ
biotropia-134	179	19	up	up	ADP
biotropia-134	179	20	to	to	PART
biotropia-134	179	21	8	8	NUM
biotropia-134	179	22	pulses	pulse	NOUN
biotropia-134	179	23	.	.	PUNCT
biotropia-134	180	1	this	this	PRON
biotropia-134	180	2	suggests	suggest	VERB
biotropia-134	180	3	that	that	SCONJ
biotropia-134	180	4	optimum	optimum	ADJ
biotropia-134	180	5	level	level	NOUN
biotropia-134	180	6	of	of	ADP
biotropia-134	180	7	electroporation	electroporation	NOUN
biotropia-134	180	8	parameters	parameter	NOUN
biotropia-134	180	9	may	may	AUX
biotropia-134	180	10	be	be	AUX
biotropia-134	180	11	species	specie	NOUN
biotropia-134	180	12	specific	specific	ADJ
biotropia-134	180	13	.	.	PUNCT
biotropia-134	181	1	finally	finally	ADV
biotropia-134	181	2	,	,	PUNCT
biotropia-134	181	3	compared	compare	VERB
biotropia-134	181	4	to	to	ADP
biotropia-134	181	5	the	the	DET
biotropia-134	181	6	three	three	NUM
biotropia-134	181	7	other	other	ADJ
biotropia-134	181	8	methods	method	NOUN
biotropia-134	181	9	examined	examine	VERB
biotropia-134	181	10	for	for	ADP
biotropia-134	181	11	humpback	humpback	NOUN
biotropia-134	181	12	grouper	grouper	NOUN
biotropia-134	181	13	as	as	SCONJ
biotropia-134	181	14	discussed	discuss	VERB
biotropia-134	181	15	above	above	ADV
biotropia-134	181	16	,	,	PUNCT
biotropia-134	181	17	electroporation	electroporation	NOUN
biotropia-134	181	18	to	to	ADP
biotropia-134	181	19	sperm	sperm	NOUN
biotropia-134	181	20	is	be	AUX
biotropia-134	181	21	a	a	DET
biotropia-134	181	22	fast	fast	ADJ
biotropia-134	181	23	,	,	PUNCT
biotropia-134	181	24	simple	simple	ADJ
biotropia-134	181	25	,	,	PUNCT
biotropia-134	181	26	and	and	CCONJ
biotropia-134	181	27	efficient	efficient	ADJ
biotropia-134	181	28	transgenic	transgenic	NOUN
biotropia-134	181	29	method	method	NOUN
biotropia-134	181	30	for	for	ADP
biotropia-134	181	31	humpback	humpback	NOUN
biotropia-134	181	32	grouper	grouper	NOUN
biotropia-134	181	33	.	.	PUNCT
biotropia-134	182	1	production	production	NOUN
biotropia-134	182	2	of	of	ADP
biotropia-134	182	3	transgenic	transgenic	NOUN
biotropia-134	182	4	humpback	humpback	NOUN
biotropia-134	182	5	grouper	grouper	NOUN
biotropia-134	182	6	using	use	VERB
biotropia-134	182	7	electroporation	electroporation	NOUN
biotropia-134	182	8	technique	technique	NOUN
biotropia-134	182	9	is	be	AUX
biotropia-134	182	10	in	in	ADP
biotropia-134	182	11	progress	progress	NOUN
biotropia-134	182	12	in	in	ADP
biotropia-134	182	13	our	our	PRON
biotropia-134	182	14	laboratory	laboratory	NOUN
biotropia-134	182	15	.	.	PUNCT
biotropia-134	183	1	electr	electr	PROPN
biotropia-134	183	2	production	production	NOUN
biotropia-134	183	3	of	of	ADP
biotropia-134	183	4	transgenic	transgenic	NOUN
biotropia-134	183	5	grouper	grouper	NOUN
biotropia-134	183	6	carrying	carry	VERB
biotropia-134	183	7	genes	gene	NOUN
biotropia-134	183	8	regulating	regulate	VERB
biotropia-134	183	9	the	the	DET
biotropia-134	183	10	important	important	ADJ
biotropia-134	183	11	traits	trait	NOUN
biotropia-134	183	12	for	for	ADP
biotropia-134	183	13	aquaculture	aquaculture	NOUN
biotropia-134	183	14	holds	hold	VERB
biotropia-134	183	15	exciting	exciting	ADJ
biotropia-134	183	16	possibilities	possibility	NOUN
biotropia-134	183	17	for	for	ADP
biotropia-134	183	18	the	the	DET
biotropia-134	183	19	future	future	NOUN
biotropia-134	183	20	,	,	PUNCT
biotropia-134	183	21	though	though	SCONJ
biotropia-134	183	22	the	the	DET
biotropia-134	183	23	cultivation	cultivation	NOUN
biotropia-134	183	24	of	of	ADP
biotropia-134	183	25	transgenic	transgenic	NOUN
biotropia-134	183	26	organisms	organism	NOUN
biotropia-134	183	27	remains	remain	VERB
biotropia-134	183	28	controversial	controversial	ADJ
biotropia-134	183	29	.	.	PUNCT
biotropia-134	183	30	.	.	PUNCT
biotropia-134	184	1	we	we	PRON
biotropia-134	184	2	thank	thank	VERB
biotropia-134	184	3	the	the	DET
biotropia-134	184	4	research	research	NOUN
biotropia-134	184	5	team	team	NOUN
biotropia-134	184	6	for	for	ADP
biotropia-134	184	7	genetic	genetic	ADJ
biotropia-134	184	8	quality	quality	NOUN
biotropia-134	184	9	improvement	improvement	NOUN
biotropia-134	184	10	of	of	ADP
biotropia-134	184	11	humpback	humpback	NOUN
biotropia-134	184	12	grouper	grouper	NOUN
biotropia-134	184	13	broodstock	broodstock	NOUN
biotropia-134	184	14	of	of	ADP
biotropia-134	184	15	rusnas	rusnas	PROPN
biotropia-134	184	16	(	(	PUNCT
biotropia-134	184	17	national	national	ADJ
biotropia-134	184	18	strategic	strategic	ADJ
biotropia-134	184	19	prime	prime	ADJ
biotropia-134	184	20	research	research	NOUN
biotropia-134	184	21	)	)	PUNCT
biotropia-134	184	22	program	program	NOUN
biotropia-134	184	23	of	of	ADP
biotropia-134	184	24	ministry	ministry	PROPN
biotropia-134	184	25	of	of	ADP
biotropia-134	184	26	research	research	PROPN
biotropia-134	184	27	and	and	CCONJ
biotropia-134	184	28	technology	technology	NOUN
biotropia-134	184	29	republic	republic	NOUN
biotropia-134	184	30	indonesia	indonesia	PROPN
biotropia-134	184	31	for	for	ADP
biotropia-134	184	32	providing	provide	VERB
biotropia-134	184	33	the	the	DET
biotropia-134	184	34	pktbpet	pktbpet	NOUN
biotropia-134	184	35	al	al	PROPN
biotropia-134	184	36	et	et	PROPN
biotropia-134	184	37	al	al	PROPN
biotropia-134	184	38	.	.	PUNCT
biotropia-134	185	1	in	in	ADP
biotropia-134	185	2	vivo	vivo	NOUN
biotropia-134	185	3	et	et	NOUN
biotropia-134	185	4	al	al	PROPN
biotropia-134	185	5	et	et	PROPN
biotropia-134	185	6	al	al	PROPN
biotropia-134	185	7	.	.	PUNCT
biotropia-134	185	8	2002	2002	NUM
biotropia-134	185	9	)	)	PUNCT
biotropia-134	185	10	.	.	PUNCT
biotropia-134	186	1	as	as	SCONJ
biotropia-134	186	2	shown	show	VERB
biotropia-134	186	3	in	in	ADP
biotropia-134	186	4	figure	figure	NOUN
biotropia-134	186	5	4	4	NUM
biotropia-134	186	6	,	,	PUNCT
biotropia-134	186	7	all	all	DET
biotropia-134	186	8	treatments	treatment	NOUN
biotropia-134	186	9	allowed	allow	VERB
biotropia-134	186	10	the	the	DET
biotropia-134	186	11	sperm	sperm	NOUN
biotropia-134	186	12	to	to	PART
biotropia-134	186	13	carry	carry	VERB
biotropia-134	186	14	the	the	DET
biotropia-134	186	15	foreign	foreign	ADJ
biotropia-134	186	16	gene	gene	NOUN
biotropia-134	186	17	.	.	PUNCT
biotropia-134	187	1	based	base	VERB
biotropia-134	187	2	on	on	ADP
biotropia-134	187	3	the	the	DET
biotropia-134	187	4	sperm	sperm	NOUN
biotropia-134	187	5	motility	motility	NOUN
biotropia-134	187	6	and	and	CCONJ
biotropia-134	187	7	results	result	NOUN
biotropia-134	187	8	of	of	ADP
biotropia-134	187	9	pcr	pcr	ADJ
biotropia-134	187	10	analysis	analysis	NOUN
biotropia-134	187	11	,	,	PUNCT
biotropia-134	187	12	10	10	NUM
biotropia-134	187	13	μg	μg	NOUN
biotropia-134	187	14	/	/	SYM
biotropia-134	187	15	ml	ml	PROPN
biotropia-134	187	16	was	be	AUX
biotropia-134	187	17	considered	consider	VERB
biotropia-134	187	18	as	as	ADP
biotropia-134	187	19	the	the	DET
biotropia-134	187	20	optimum	optimum	ADJ
biotropia-134	187	21	dna	dna	NOUN
biotropia-134	187	22	concentration	concentration	NOUN
biotropia-134	187	23	for	for	ADP
biotropia-134	187	24	electroporation	electroporation	NOUN
biotropia-134	187	25	of	of	ADP
biotropia-134	187	26	humpback	humpback	NOUN
biotropia-134	187	27	grouper	grouper	NOUN
biotropia-134	187	28	.	.	PUNCT
biotropia-134	188	1	this	this	DET
biotropia-134	188	2	dna	dna	PROPN
biotropia-134	188	3	concentration	concentration	NOUN
biotropia-134	188	4	was	be	AUX
biotropia-134	188	5	lower	low	ADJ
biotropia-134	188	6	than	than	ADP
biotropia-134	188	7	that	that	PRON
biotropia-134	188	8	used	use	VERB
biotropia-134	188	9	for	for	ADP
biotropia-134	188	10	sea	sea	NOUN
biotropia-134	188	11	bream	bream	NOUN
biotropia-134	188	12	,	,	PUNCT
biotropia-134	188	13	25	25	NUM
biotropia-134	188	14	μg	μg	NOUN
biotropia-134	188	15	/	/	SYM
biotropia-134	188	16	ml	ml	PROPN
biotropia-134	188	17	(	(	PUNCT
biotropia-134	188	18	lu	lu	NOUN
biotropia-134	188	19	oporation	oporation	NOUN
biotropia-134	188	20	to	to	ADP
biotropia-134	188	21	sperm	sperm	NOUN
biotropia-134	188	22	was	be	AUX
biotropia-134	188	23	considered	consider	VERB
biotropia-134	188	24	as	as	ADP
biotropia-134	188	25	an	an	DET
biotropia-134	188	26	appropriate	appropriate	ADJ
biotropia-134	188	27	approach	approach	NOUN
biotropia-134	188	28	by	by	ADP
biotropia-134	188	29	means	mean	NOUN
biotropia-134	188	30	of	of	ADP
biotropia-134	188	31	efficacy	efficacy	NOUN
biotropia-134	188	32	and	and	CCONJ
biotropia-134	188	33	simplicity	simplicity	NOUN
biotropia-134	188	34	,	,	PUNCT
biotropia-134	188	35	to	to	PART
biotropia-134	188	36	generate	generate	VERB
biotropia-134	188	37	transgenic	transgenic	NOUN
biotropia-134	188	38	humpback	humpback	NOUN
biotropia-134	188	39	grouper	grouper	NOUN
biotropia-134	188	40	.	.	PUNCT
biotropia-134	189	1	optimum	optimum	ADJ
biotropia-134	189	2	dna	dna	PROPN
biotropia-134	189	3	concentration	concentration	NOUN
biotropia-134	189	4	for	for	ADP
biotropia-134	189	5	electroporation	electroporation	NOUN
biotropia-134	189	6	was	be	AUX
biotropia-134	189	7	10	10	NUM
biotropia-134	189	8	μg	μg	NOUN
biotropia-134	189	9	/	/	SYM
biotropia-134	189	10	ml	ml	NOUN
biotropia-134	189	11	.	.	PUNCT
biotropia-134	190	1	conclusions	conclusion	NOUN
biotropia-134	190	2	acknowledgements	acknowledgement	VERB
biotropia-134	190	3	gene	gene	NOUN
biotropia-134	190	4	transfer	transfer	NOUN
biotropia-134	190	5	method	method	NOUN
biotropia-134	190	6	in	in	ADP
biotropia-134	190	7	humpback	humpback	NOUN
biotropia-134	190	8	grouper	grouper	NOUN
biotropia-134	190	9	alimuddin	alimuddin	PROPN
biotropia-134	190	10	.et	.et	PUNCT
biotropia-134	191	1	al	al	PROPN
biotropia-134	191	2	22	22	NUM
biotropia-134	191	3	biotropia	biotropia	ADJ
biotropia-134	191	4	vol	vol	NOUN
biotropia-134	191	5	.	.	PROPN
biotropia-134	192	1	18	18	NUM
biotropia-134	192	2	no	no	NOUN
biotropia-134	192	3	.	.	NOUN
biotropia-134	192	4	1	1	NUM
biotropia-134	192	5	,	,	PUNCT
biotropia-134	192	6	2011	2011	NUM
biotropia-134	192	7	ktgh	ktgh	NOUN
biotropia-134	192	8	plasmid	plasmid	NOUN
biotropia-134	192	9	;	;	PUNCT
biotropia-134	192	10	head	head	NOUN
biotropia-134	192	11	of	of	ADP
biotropia-134	192	12	the	the	DET
biotropia-134	192	13	department	department	NOUN
biotropia-134	192	14	of	of	ADP
biotropia-134	192	15	aquaculture	aquaculture	NOUN
biotropia-134	192	16	,	,	PUNCT
biotropia-134	192	17	faculty	faculty	NOUN
biotropia-134	192	18	of	of	ADP
biotropia-134	192	19	fisheries	fishery	NOUN
biotropia-134	192	20	and	and	CCONJ
biotropia-134	192	21	marine	marine	ADJ
biotropia-134	192	22	sciences	science	NOUN
biotropia-134	192	23	,	,	PUNCT
biotropia-134	192	24	bogor	bogor	PROPN
biotropia-134	192	25	agricultural	agricultural	ADJ
biotropia-134	192	26	university	university	NOUN
biotropia-134	192	27	for	for	ADP
biotropia-134	192	28	supporting	support	VERB
biotropia-134	192	29	laboratory	laboratory	NOUN
biotropia-134	192	30	facilities	facility	NOUN
biotropia-134	192	31	;	;	PUNCT
biotropia-134	192	32	our	our	PRON
biotropia-134	192	33	thanks	thank	NOUN
biotropia-134	192	34	are	be	AUX
biotropia-134	192	35	also	also	ADV
biotropia-134	192	36	due	due	ADP
biotropia-134	192	37	to	to	ADP
biotropia-134	192	38	the	the	DET
biotropia-134	192	39	staffs	staff	NOUN
biotropia-134	192	40	of	of	ADP
biotropia-134	192	41	situbondo	situbondo	PROPN
biotropia-134	192	42	brachishwater	brachishwater	NOUN
biotropia-134	192	43	aquaculture	aquaculture	NOUN
biotropia-134	192	44	center	center	NOUN
biotropia-134	192	45	,	,	PUNCT
biotropia-134	192	46	especially	especially	ADV
biotropia-134	192	47	the	the	DET
biotropia-134	192	48	grouper	grouper	ADJ
biotropia-134	192	49	parent	parent	NOUN
biotropia-134	192	50	team	team	NOUN
biotropia-134	192	51	for	for	ADP
biotropia-134	192	52	their	their	PRON
biotropia-134	192	53	cooperation	cooperation	NOUN
biotropia-134	192	54	and	and	CCONJ
biotropia-134	192	55	technical	technical	ADJ
biotropia-134	192	56	support	support	NOUN
biotropia-134	192	57	in	in	ADP
biotropia-134	192	58	broodstock	broodstock	NOUN
biotropia-134	192	59	handling	handling	NOUN
biotropia-134	192	60	.	.	PUNCT
biotropia-134	193	1	references	reference	NOUN
biotropia-134	193	2	alimuddin	alimuddin	PROPN
biotropia-134	193	3	,	,	PUNCT
biotropia-134	193	4	nugrahani	nugrahani	PROPN
biotropia-134	193	5	w	w	PROPN
biotropia-134	193	6	,	,	PUNCT
biotropia-134	193	7	aliah	aliah	ADV
biotropia-134	193	8	rs	r	NOUN
biotropia-134	193	9	,	,	PUNCT
biotropia-134	193	10	sumantadinata	sumantadinata	NOUN
biotropia-134	193	11	k	k	PROPN
biotropia-134	193	12	,	,	PUNCT
biotropia-134	193	13	faizal	faizal	PROPN
biotropia-134	193	14	i	i	PROPN
biotropia-134	193	15	,	,	PUNCT
biotropia-134	193	16	carman	carman	PROPN
biotropia-134	193	17	o	o	PROPN
biotropia-134	193	18	and	and	CCONJ
biotropia-134	193	19	g.	g.	PROPN
biotropia-134	193	20	yoshizaki	yoshizaki	PROPN
biotropia-134	193	21	.	.	PUNCT
biotropia-134	193	22	.	.	PUNCT
biotropia-134	194	1	jurnal	jurnal	ADJ
biotropia-134	194	2	riset	riset	PROPN
biotropia-134	194	3	akuakultur	akuakultur	NOUN
biotropia-134	194	4	,	,	PUNCT
biotropia-134	194	5	2	2	NUM
biotropia-134	194	6	:	:	SYM
biotropia-134	194	7	199	199	NUM
biotropia-134	194	8	-	-	SYM
biotropia-134	194	9	209	209	NUM
biotropia-134	194	10	.	.	PUNCT
biotropia-134	195	1	(	(	PUNCT
biotropia-134	195	2	in	in	ADP
biotropia-134	195	3	indonesian	indonesian	ADJ
biotropia-134	195	4	)	)	PUNCT
biotropia-134	195	5	.	.	PUNCT
biotropia-134	196	1	alimuddin	alimuddin	PROPN
biotropia-134	196	2	,	,	PUNCT
biotropia-134	196	3	yoshizaki	yoshizaki	VERB
biotropia-134	196	4	g	g	PROPN
biotropia-134	196	5	,	,	PUNCT
biotropia-134	196	6	carman	carman	PROPN
biotropia-134	196	7	o.	o.	PROPN
biotropia-134	196	8	and	and	CCONJ
biotropia-134	196	9	k.	k.	PROPN
biotropia-134	196	10	sumantadinata	sumantadinata	PROPN
biotropia-134	196	11	.	.	PUNCT
biotropia-134	197	1	2003	2003	NUM
biotropia-134	197	2	.	.	PUNCT
biotropia-134	198	1	application	application	NOUN
biotropia-134	198	2	of	of	ADP
biotropia-134	198	3	gene	gene	NOUN
biotropia-134	198	4	transfer	transfer	NOUN
biotropia-134	198	5	technology	technology	NOUN
biotropia-134	198	6	in	in	ADP
biotropia-134	198	7	aquaculture	aquaculture	NOUN
biotropia-134	198	8	.	.	PUNCT
biotropia-134	199	1	jurnal	jurnal	PROPN
biotropia-134	199	2	akuakultur	akuakultur	PROPN
biotropia-134	199	3	indonesia	indonesia	PROPN
biotropia-134	199	4	,	,	PUNCT
biotropia-134	199	5	2	2	NUM
biotropia-134	199	6	:	:	SYM
biotropia-134	199	7	41	41	NUM
biotropia-134	199	8	-	-	SYM
biotropia-134	199	9	50	50	NUM
biotropia-134	199	10	.	.	PUNCT
biotropia-134	200	1	(	(	PUNCT
biotropia-134	200	2	in	in	ADP
biotropia-134	200	3	indonesian	indonesian	ADJ
biotropia-134	200	4	)	)	PUNCT
biotropia-134	200	5	.	.	PUNCT
biotropia-134	201	1	alimuddin	alimuddin	PROPN
biotropia-134	201	2	,	,	PUNCT
biotropia-134	201	3	yoshizaki	yoshizaki	VERB
biotropia-134	201	4	g	g	PROPN
biotropia-134	201	5	,	,	PUNCT
biotropia-134	201	6	kiron	kiron	PROPN
biotropia-134	201	7	v	v	NOUN
biotropia-134	201	8	,	,	PUNCT
biotropia-134	201	9	satoh	satoh	NOUN
biotropia-134	201	10	s	s	X
biotropia-134	201	11	and	and	CCONJ
biotropia-134	201	12	t.	t.	NOUN
biotropia-134	201	13	transgenic	transgenic	NOUN
biotropia-134	201	14	research	research	NOUN
biotropia-134	201	15	,	,	PUNCT
biotropia-134	201	16	14	14	NUM
biotropia-134	201	17	:	:	SYM
biotropia-134	201	18	159	159	NUM
biotropia-134	201	19	165	165	NUM
biotropia-134	201	20	.	.	PUNCT
biotropia-134	202	1	alimuddin	alimuddin	PROPN
biotropia-134	202	2	,	,	PUNCT
biotropia-134	202	3	kiron	kiron	PROPN
biotropia-134	202	4	v	v	NOUN
biotropia-134	202	5	,	,	PUNCT
biotropia-134	202	6	satoh	satoh	NOUN
biotropia-134	202	7	s	s	PROPN
biotropia-134	202	8	,	,	PUNCT
biotropia-134	202	9	takeuchi	takeuchi	PROPN
biotropia-134	202	10	t	t	PROPN
biotropia-134	202	11	and	and	CCONJ
biotropia-134	202	12	g.	g.	PROPN
biotropia-134	202	13	yoshizaki	yoshizaki	PROPN
biotropia-134	202	14	.	.	PUNCT
biotropia-134	203	1	2008	2008	NUM
biotropia-134	203	2	.	.	PUNCT
biotropia-134	204	1	cloning	cloning	NOUN
biotropia-134	204	2	and	and	CCONJ
biotropia-134	204	3	over	over	ADP
biotropia-134	204	4	-	-	PUNCT
biotropia-134	204	5	expression	expression	NOUN
biotropia-134	204	6	of	of	ADP
biotropia-134	204	7	a	a	DET
biotropia-134	204	8	masu	masu	PROPN
biotropia-134	204	9	salmon	salmon	NOUN
biotropia-134	204	10	(	(	PUNCT
biotropia-134	204	11	)	)	PUNCT
biotropia-134	204	12	fatty	fatty	NOUN
biotropia-134	204	13	acid	acid	NOUN
biotropia-134	204	14	elongase	elongase	NOUN
biotropia-134	204	15	-	-	PUNCT
biotropia-134	204	16	like	like	ADJ
biotropia-134	204	17	gene	gene	NOUN
biotropia-134	204	18	in	in	ADP
biotropia-134	204	19	zebrafish	zebrafish	ADJ
biotropia-134	204	20	.	.	PUNCT
biotropia-134	204	21	aquaculture	aquaculture	NOUN
biotropia-134	204	22	,	,	PUNCT
biotropia-134	204	23	282	282	NUM
biotropia-134	204	24	:	:	SYM
biotropia-134	204	25	13	13	NUM
biotropia-134	204	26	-	-	SYM
biotropia-134	204	27	18	18	NUM
biotropia-134	204	28	.	.	PUNCT
biotropia-134	204	29	anonymous	anonymous	ADJ
biotropia-134	204	30	,	,	PUNCT
biotropia-134	204	31	2006	2006	NUM
biotropia-134	204	32	.	.	PUNCT
biotropia-134	205	1	instruction	instruction	NOUN
biotropia-134	205	2	manual	manual	NOUN
biotropia-134	205	3	:	:	PUNCT
biotropia-134	205	4	gene	gene	NOUN
biotropia-134	205	5	pulser	pulser	PROPN
biotropia-134	205	6	xcell	xcell	PROPN
biotropia-134	206	1	electroporation	electroporation	NOUN
biotropia-134	206	2	system	system	NOUN
biotropia-134	206	3	,	,	PUNCT
biotropia-134	206	4	biorad	biorad	ADJ
biotropia-134	206	5	catalog	catalog	NOUN
biotropia-134	206	6	number	number	NOUN
biotropia-134	206	7	74	74	NUM
biotropia-134	206	8	,	,	PUNCT
biotropia-134	206	9	biorad	biorad	NOUN
biotropia-134	206	10	office	office	NOUN
biotropia-134	206	11	.	.	PUNCT
biotropia-134	207	1	ath	ath	NOUN
biotropia-134	207	2	-	-	PUNCT
biotropia-134	207	3	thar	thar	PROPN
biotropia-134	207	4	mf	mf	PROPN
biotropia-134	207	5	.	.	PROPN
biotropia-134	207	6	2007	2007	NUM
biotropia-134	207	7	.	.	PUNCT
biotropia-134	208	1	effectiveness	effectiveness	NOUN
biotropia-134	208	2	of	of	ADP
biotropia-134	208	3	oryzias	oryzia	NOUN
biotropia-134	208	4	latipes	latipe	NOUN
biotropia-134	208	5	using	use	VERB
biotropia-134	208	6	hrgfp	hrgfp	ADV
biotropia-134	208	7	(	(	PUNCT
biotropia-134	208	8	humanized	humanize	VERB
biotropia-134	208	9	renilla	renilla	NOUN
biotropia-134	208	10	reniformis	reniformis	PROPN
biotropia-134	208	11	green	green	ADJ
biotropia-134	208	12	fluorescent	fluorescent	ADJ
biotropia-134	208	13	protein	protein	NOUN
biotropia-134	208	14	)	)	PUNCT
biotropia-134	208	15	gene	gene	NOUN
biotropia-134	208	16	as	as	ADP
biotropia-134	208	17	a	a	DET
biotropia-134	208	18	marker	marker	NOUN
biotropia-134	208	19	in	in	ADP
biotropia-134	208	20	catfish	catfish	NOUN
biotropia-134	208	21	clarias	claria	NOUN
biotropia-134	208	22	sp	sp	ADP
biotropia-134	208	23	f0	f0	PROPN
biotropia-134	208	24	generation	generation	NOUN
biotropia-134	208	25	.	.	PUNCT
biotropia-134	209	1	undergraduate	undergraduate	ADJ
biotropia-134	209	2	thesis	thesis	NOUN
biotropia-134	209	3	.	.	PUNCT
biotropia-134	210	1	department	department	NOUN
biotropia-134	210	2	of	of	ADP
biotropia-134	210	3	aquacuture	aquacuture	PROPN
biotropia-134	210	4	,	,	PUNCT
biotropia-134	210	5	faculty	faculty	NOUN
biotropia-134	210	6	of	of	ADP
biotropia-134	210	7	fisheries	fishery	NOUN
biotropia-134	210	8	and	and	CCONJ
biotropia-134	210	9	marine	marine	ADJ
biotropia-134	210	10	sciences	science	NOUN
biotropia-134	210	11	,	,	PUNCT
biotropia-134	210	12	bogor	bogor	X
biotropia-134	210	13	agricultural	agricultural	ADJ
biotropia-134	210	14	university	university	NOUN
biotropia-134	210	15	.	.	PUNCT
biotropia-134	211	1	(	(	PUNCT
biotropia-134	211	2	in	in	ADP
biotropia-134	211	3	indonesian	indonesian	ADJ
biotropia-134	211	4	)	)	PUNCT
biotropia-134	211	5	.	.	PUNCT
biotropia-134	212	1	chen	chen	PROPN
biotropia-134	212	2	tt	tt	PROPN
biotropia-134	212	3	,	,	PUNCT
biotropia-134	212	4	kight	kight	PROPN
biotropia-134	212	5	k	k	PROPN
biotropia-134	212	6	,	,	PUNCT
biotropia-134	212	7	lin	lin	PROPN
biotropia-134	212	8	cm	cm	PROPN
biotropia-134	212	9	,	,	PUNCT
biotropia-134	212	10	powers	power	NOUN
biotropia-134	212	11	da	da	PROPN
biotropia-134	212	12	,	,	PUNCT
biotropia-134	212	13	hayat	hayat	PROPN
biotropia-134	212	14	m	m	PROPN
biotropia-134	212	15	,	,	PUNCT
biotropia-134	212	16	chatakondi	chatakondi	NOUN
biotropia-134	212	17	n	n	CCONJ
biotropia-134	212	18	,	,	PUNCT
biotropia-134	212	19	ramboux	ramboux	NOUN
biotropia-134	212	20	ac	ac	PROPN
biotropia-134	212	21	,	,	PUNCT
biotropia-134	212	22	duncan	duncan	PROPN
biotropia-134	212	23	pl	pl	PROPN
biotropia-134	212	24	and	and	CCONJ
biotropia-134	212	25	ra	ra	PROPN
biotropia-134	212	26	dunham	dunham	PROPN
biotropia-134	212	27	.	.	PUNCT
biotropia-134	213	1	1993	1993	NUM
biotropia-134	213	2	.	.	PUNCT
biotropia-134	214	1	expression	expression	NOUN
biotropia-134	214	2	and	and	CCONJ
biotropia-134	214	3	inheritance	inheritance	NOUN
biotropia-134	214	4	of	of	ADP
biotropia-134	214	5	rsvltr	rsvltr	NOUN
biotropia-134	214	6	-	-	PUNCT
biotropia-134	214	7	rtgh1	rtgh1	NOUN
biotropia-134	214	8	complemetary	complemetary	ADJ
biotropia-134	214	9	dna	dna	NOUN
biotropia-134	214	10	in	in	ADP
biotropia-134	214	11	the	the	DET
biotropia-134	214	12	transgenic	transgenic	NOUN
biotropia-134	214	13	common	common	ADJ
biotropia-134	214	14	carp	carp	NOUN
biotropia-134	214	15	,	,	PUNCT
biotropia-134	214	16	.	.	PUNCT
biotropia-134	215	1	molecular	molecular	ADJ
biotropia-134	215	2	biology	biology	NOUN
biotropia-134	215	3	and	and	CCONJ
biotropia-134	215	4	biotechnology	biotechnology	NOUN
biotropia-134	215	5	,	,	PUNCT
biotropia-134	215	6	2:88	2:88	NUM
biotropia-134	215	7	-	-	SYM
biotropia-134	215	8	95	95	NUM
biotropia-134	215	9	.	.	PUNCT
biotropia-134	216	1	devlin	devlin	PROPN
biotropia-134	216	2	rh	rh	PROPN
biotropia-134	216	3	,	,	PUNCT
biotropia-134	216	4	yesaki	yesaki	VERB
biotropia-134	216	5	ty	ty	PRON
biotropia-134	216	6	,	,	PUNCT
biotropia-134	216	7	donaldson	donaldson	PROPN
biotropia-134	216	8	em	em	PRON
biotropia-134	216	9	,	,	PUNCT
biotropia-134	216	10	du	du	X
biotropia-134	216	11	sj	sj	X
biotropia-134	216	12	and	and	CCONJ
biotropia-134	216	13	cl	cl	NOUN
biotropia-134	216	14	hew	hew	VERB
biotropia-134	216	15	.	.	PUNCT
biotropia-134	216	16	1995	1995	NUM
biotropia-134	216	17	.	.	PUNCT
biotropia-134	217	1	production	production	NOUN
biotropia-134	217	2	of	of	ADP
biotropia-134	217	3	germline	germline	NOUN
biotropia-134	217	4	transgenic	transgenic	NOUN
biotropia-134	217	5	pacific	pacific	ADJ
biotropia-134	217	6	salmonids	salmonid	NOUN
biotropia-134	217	7	with	with	ADP
biotropia-134	217	8	dramatically	dramatically	ADV
biotropia-134	217	9	increased	increase	VERB
biotropia-134	217	10	growth	growth	NOUN
biotropia-134	217	11	performance	performance	NOUN
biotropia-134	217	12	.	.	PUNCT
biotropia-134	218	1	canadian	canadian	ADJ
biotropia-134	218	2	journal	journal	PROPN
biotropia-134	218	3	of	of	ADP
biotropia-134	218	4	fisheries	fishery	NOUN
biotropia-134	218	5	aquatic	aquatic	ADJ
biotropia-134	218	6	sciences	science	NOUN
biotropia-134	218	7	,	,	PUNCT
biotropia-134	218	8	52	52	NUM
biotropia-134	218	9	:	:	SYM
biotropia-134	218	10	1376	1376	NUM
biotropia-134	218	11	1384	1384	NUM
biotropia-134	218	12	.	.	PUNCT
biotropia-134	219	1	lu	lu	PROPN
biotropia-134	219	2	jk	jk	PROPN
biotropia-134	219	3	,	,	PUNCT
biotropia-134	219	4	fu	fu	PROPN
biotropia-134	219	5	bh	bh	PROPN
biotropia-134	219	6	,	,	PUNCT
biotropia-134	219	7	wu	wu	PROPN
biotropia-134	219	8	jl	jl	PROPN
biotropia-134	219	9	and	and	CCONJ
biotropia-134	219	10	tt	tt	PROPN
biotropia-134	219	11	chen	chen	PROPN
biotropia-134	219	12	.	.	PUNCT
biotropia-134	219	13	2002	2002	NUM
biotropia-134	219	14	.	.	PUNCT
biotropia-134	220	1	production	production	NOUN
biotropia-134	220	2	of	of	ADP
biotropia-134	220	3	transgenic	transgenic	NOUN
biotropia-134	220	4	silver	silver	NOUN
biotropia-134	220	5	sea	sea	NOUN
biotropia-134	220	6	bream	bream	NOUN
biotropia-134	220	7	(	(	PUNCT
biotropia-134	220	8	)	)	PUNCT
biotropia-134	220	9	by	by	ADP
biotropia-134	220	10	different	different	ADJ
biotropia-134	220	11	gene	gene	NOUN
biotropia-134	220	12	transfer	transfer	NOUN
biotropia-134	220	13	methods	method	NOUN
biotropia-134	220	14	.	.	PUNCT
biotropia-134	221	1	marine	marine	PROPN
biotropia-134	221	2	biotechnology	biotechnology	NOUN
biotropia-134	221	3	,	,	PUNCT
biotropia-134	221	4	4	4	NUM
biotropia-134	221	5	:	:	SYM
biotropia-134	221	6	328	328	NUM
biotropia-134	221	7	-	-	SYM
biotropia-134	221	8	337	337	NUM
biotropia-134	221	9	.	.	PUNCT
biotropia-134	222	1	patil	patil	PROPN
biotropia-134	222	2	jg	jg	PROPN
biotropia-134	222	3	and	and	CCONJ
biotropia-134	222	4	khoo	khoo	PROPN
biotropia-134	222	5	hw	hw	PROPN
biotropia-134	222	6	.	.	PROPN
biotropia-134	222	7	1996	1996	NUM
biotropia-134	222	8	.	.	PUNCT
biotropia-134	223	1	nuclear	nuclear	ADJ
biotropia-134	223	2	internalization	internalization	NOUN
biotropia-134	223	3	of	of	ADP
biotropia-134	223	4	foreign	foreign	ADJ
biotropia-134	223	5	dna	dna	NOUN
biotropia-134	223	6	by	by	ADP
biotropia-134	223	7	zebrafish	zebrafish	ADJ
biotropia-134	223	8	spermatozoa	spermatozoon	NOUN
biotropia-134	223	9	and	and	CCONJ
biotropia-134	223	10	its	its	PRON
biotropia-134	223	11	enhancement	enhancement	NOUN
biotropia-134	223	12	by	by	ADP
biotropia-134	223	13	electroporation	electroporation	NOUN
biotropia-134	223	14	.	.	PUNCT
biotropia-134	224	1	journal	journal	NOUN
biotropia-134	224	2	of	of	ADP
biotropia-134	224	3	experimental	experimental	ADJ
biotropia-134	224	4	zoology	zoology	NOUN
biotropia-134	224	5	,	,	PUNCT
biotropia-134	224	6	274	274	NUM
biotropia-134	224	7	:	:	SYM
biotropia-134	224	8	121	121	NUM
biotropia-134	224	9	-	-	SYM
biotropia-134	224	10	129	129	NUM
biotropia-134	224	11	.	.	PUNCT
biotropia-134	225	1	rambubu	rambubu	NOUN
biotropia-134	225	2	km	km	PROPN
biotropia-134	225	3	,	,	PUNCT
biotropia-134	225	4	rao	rao	NOUN
biotropia-134	225	5	shn	shn	PROPN
biotropia-134	225	6	and	and	CCONJ
biotropia-134	225	7	nm	nm	ADJ
biotropia-134	225	8	rao	rao	NOUN
biotropia-134	225	9	.	.	PUNCT
biotropia-134	225	10	2005	2005	NUM
biotropia-134	225	11	.	.	PUNCT
biotropia-134	226	1	efficient	efficient	ADJ
biotropia-134	226	2	expression	expression	NOUN
biotropia-134	226	3	of	of	ADP
biotropia-134	226	4	transgene	transgene	NOUN
biotropia-134	226	5	in	in	ADP
biotropia-134	226	6	adult	adult	NOUN
biotropia-134	226	7	zebrafish	zebrafish	NOUN
biotropia-134	226	8	by	by	ADP
biotropia-134	226	9	electroporation	electroporation	NOUN
biotropia-134	226	10	.	.	PUNCT
biotropia-134	227	1	bmc	bmc	PROPN
biotropia-134	227	2	biotechnology	biotechnology	NOUN
biotropia-134	227	3	,	,	PUNCT
biotropia-134	227	4	5:29	5:29	NUM
biotropia-134	227	5	.	.	PUNCT
biotropia-134	228	1	rusnas	rusna	NOUN
biotropia-134	228	2	of	of	ADP
biotropia-134	228	3	humpback	humpback	NOUN
biotropia-134	228	4	grouper	grouper	NOUN
biotropia-134	228	5	broodstock	broodstock	PROPN
biotropia-134	228	6	research	research	NOUN
biotropia-134	228	7	team	team	NOUN
biotropia-134	228	8	.	.	PUNCT
biotropia-134	229	1	2007	2007	NUM
biotropia-134	229	2	.	.	PUNCT
biotropia-134	230	1	final	final	ADJ
biotropia-134	230	2	report	report	NOUN
biotropia-134	230	3	on	on	ADP
biotropia-134	230	4	genetic	genetic	ADJ
biotropia-134	230	5	improvement	improvement	NOUN
biotropia-134	230	6	of	of	ADP
biotropia-134	230	7	humpback	humpback	NOUN
biotropia-134	230	8	grouper	grouper	NOUN
biotropia-134	230	9	broodstock	broodstock	NOUN
biotropia-134	230	10	.	.	PUNCT
biotropia-134	231	1	national	national	ADJ
biotropia-134	231	2	strategic	strategic	ADJ
biotropia-134	231	3	primer	primer	NOUN
biotropia-134	231	4	research	research	NOUN
biotropia-134	231	5	program	program	NOUN
biotropia-134	231	6	of	of	ADP
biotropia-134	231	7	the	the	DET
biotropia-134	231	8	ministry	ministry	PROPN
biotropia-134	231	9	of	of	ADP
biotropia-134	231	10	research	research	NOUN
biotropia-134	231	11	and	and	CCONJ
biotropia-134	231	12	technology	technology	NOUN
biotropia-134	231	13	,	,	PUNCT
biotropia-134	231	14	republic	republic	NOUN
biotropia-134	231	15	indonesia	indonesia	PROPN
biotropia-134	231	16	.	.	PUNCT
biotropia-134	232	1	78	78	NUM
biotropia-134	232	2	p.	p.	NOUN
biotropia-134	232	3	sarmasik	sarmasik	PROPN
biotropia-134	232	4	a	a	PROPN
biotropia-134	232	5	,	,	PUNCT
biotropia-134	232	6	jang	jang	PROPN
biotropia-134	232	7	ik	ik	PROPN
biotropia-134	232	8	,	,	PUNCT
biotropia-134	232	9	chung	chung	PROPN
biotropia-134	232	10	cz	cz	PROPN
biotropia-134	232	11	,	,	PUNCT
biotropia-134	232	12	lu	lu	PROPN
biotropia-134	232	13	jk	jk	PROPN
biotropia-134	232	14	and	and	CCONJ
biotropia-134	232	15	tt	tt	PROPN
biotropia-134	232	16	chen	chen	PROPN
biotropia-134	232	17	.	.	PUNCT
biotropia-134	233	1	2001	2001	NUM
biotropia-134	233	2	.	.	PUNCT
biotropia-134	234	1	transgenic	transgenic	NOUN
biotropia-134	234	2	live	live	ADV
biotropia-134	234	3	-	-	PUNCT
biotropia-134	234	4	bearing	bear	VERB
biotropia-134	234	5	fish	fish	NOUN
biotropia-134	234	6	and	and	CCONJ
biotropia-134	234	7	crustacean	crustacean	ADJ
biotropia-134	234	8	produced	produce	VERB
biotropia-134	234	9	by	by	ADP
biotropia-134	234	10	transforming	transform	VERB
biotropia-134	234	11	immature	immature	ADJ
biotropia-134	234	12	gonads	gonad	NOUN
biotropia-134	234	13	with	with	ADP
biotropia-134	234	14	replication	replication	NOUN
biotropia-134	234	15	-	-	PUNCT
biotropia-134	234	16	defective	defective	ADJ
biotropia-134	234	17	pantropic	pantropic	ADJ
biotropia-134	234	18	retroviral	retroviral	ADJ
biotropia-134	234	19	vector	vector	NOUN
biotropia-134	234	20	,	,	PUNCT
biotropia-134	234	21	3	3	NUM
biotropia-134	234	22	:	:	SYM
biotropia-134	234	23	470	470	NUM
biotropia-134	234	24	-	-	SYM
biotropia-134	234	25	477	477	NUM
biotropia-134	234	26	.	.	PUNCT
biotropia-134	234	27	sin	sin	PROPN
biotropia-134	234	28	fyt	fyt	PROPN
biotropia-134	234	29	,	,	PUNCT
biotropia-134	234	30	bartley	bartley	PROPN
biotropia-134	234	31	al	al	PROPN
biotropia-134	234	32	,	,	PUNCT
biotropia-134	234	33	walker	walker	PROPN
biotropia-134	234	34	sp	sp	PROPN
biotropia-134	234	35	,	,	PUNCT
biotropia-134	234	36	sin	sin	PROPN
biotropia-134	234	37	il	il	PROPN
biotropia-134	234	38	,	,	PUNCT
biotropia-134	234	39	symonds	symonds	PROPN
biotropia-134	234	40	je	je	PROPN
biotropia-134	234	41	,	,	PUNCT
biotropia-134	234	42	hawke	hawke	NOUN
biotropia-134	234	43	l	l	NOUN
biotropia-134	234	44	and	and	CCONJ
biotropia-134	234	45	cl	cl	NOUN
biotropia-134	234	46	hopkins	hopkin	NOUN
biotropia-134	234	47	.	.	PROPN
biotropia-134	234	48	1993	1993	NUM
biotropia-134	234	49	.	.	PUNCT
biotropia-134	235	1	gene	gene	NOUN
biotropia-134	235	2	transfer	transfer	NOUN
biotropia-134	235	3	in	in	ADP
biotropia-134	235	4	chinook	chinook	NOUN
biotropia-134	235	5	salmon	salmon	NOUN
biotropia-134	235	6	(	(	PUNCT
biotropia-134	235	7	)	)	PUNCT
biotropia-134	235	8	by	by	ADP
biotropia-134	235	9	electroporating	electroporate	VERB
biotropia-134	235	10	sperm	sperm	NOUN
biotropia-134	235	11	in	in	ADP
biotropia-134	235	12	the	the	DET
biotropia-134	235	13	presence	presence	NOUN
biotropia-134	235	14	of	of	ADP
biotropia-134	235	15	prsv	prsv	ADV
biotropia-134	235	16	-	-	PUNCT
biotropia-134	235	17	laz	laz	NOUN
biotropia-134	235	18	dna	dna	PROPN
biotropia-134	235	19	.	.	PUNCT
biotropia-134	235	20	aquaculture	aquaculture	NOUN
biotropia-134	235	21	,	,	PUNCT
biotropia-134	235	22	111:57	111:57	PROPN
biotropia-134	235	23	-	-	SYM
biotropia-134	235	24	69	69	NUM
biotropia-134	235	25	.	.	PUNCT
biotropia-134	236	1	sin	sin	PROPN
biotropia-134	236	2	fyt	fyt	PROPN
biotropia-134	236	3	,	,	PUNCT
biotropia-134	236	4	walker	walker	PROPN
biotropia-134	236	5	sp	sp	PROPN
biotropia-134	236	6	,	,	PUNCT
biotropia-134	236	7	symonds	symonds	PROPN
biotropia-134	236	8	je	je	PROPN
biotropia-134	236	9	and	and	CCONJ
biotropia-134	236	10	il	il	PROPN
biotropia-134	236	11	sin	sin	PROPN
biotropia-134	236	12	.	.	PUNCT
biotropia-134	237	1	1994	1994	NUM
biotropia-134	237	2	.	.	PUNCT
biotropia-134	238	1	sperm	sperm	NOUN
biotropia-134	238	2	-	-	PUNCT
biotropia-134	238	3	mediated	mediate	VERB
biotropia-134	238	4	gene	gene	NOUN
biotropia-134	238	5	transfer	transfer	NOUN
biotropia-134	238	6	in	in	ADP
biotropia-134	238	7	chinook	chinook	NOUN
biotropia-134	238	8	salmon	salmon	NOUN
biotropia-134	238	9	.	.	PUNCT
biotropia-134	239	1	online	online	ADJ
biotropia-134	239	2	symposium	symposium	NOUN
biotropia-134	239	3	paper	paper	NOUN
biotropia-134	239	4	,	,	PUNCT
biotropia-134	239	5	international	international	ADJ
biotropia-134	239	6	congress	congress	PROPN
biotropia-134	239	7	of	of	ADP
biotropia-134	239	8	fish	fish	NOUN
biotropia-134	239	9	biology	biology	NOUN
biotropia-134	239	10	.	.	PUNCT
biotropia-134	240	1	vancouver	vancouver	PROPN
biotropia-134	240	2	-	-	PUNCT
biotropia-134	240	3	canada	canada	PROPN
biotropia-134	240	4	,	,	PUNCT
biotropia-134	240	5	360	360	NUM
biotropia-134	240	6	-	-	SYM
biotropia-134	240	7	365	365	NUM
biotropia-134	240	8	.	.	PUNCT
biotropia-134	241	1	http://www.biologybrowser.org/bb/subject/	http://www.biologybrowser.org/bb/subject/	NOUN
biotropia-134	241	2	…	…	PUNCT
biotropia-134	241	3	/index251.shtml	/index251.shtml	NUM
biotropia-134	241	4	.	.	PUNCT
biotropia-134	242	1	august	august	PROPN
biotropia-134	242	2	14	14	NUM
biotropia-134	242	3	,	,	PUNCT
biotropia-134	242	4	2009	2009	NUM
biotropia-134	242	5	.	.	PUNCT
biotropia-134	243	1	2007	2007	NUM
biotropia-134	243	2	.	.	PUNCT
biotropia-134	244	1	isolation	isolation	NOUN
biotropia-134	244	2	and	and	CCONJ
biotropia-134	244	3	characterization	characterization	NOUN
biotropia-134	244	4	of	of	ADP
biotropia-134	244	5	β	β	NOUN
biotropia-134	244	6	-	-	ADJ
biotropia-134	244	7	actin	actin	ADJ
biotropia-134	244	8	promoter	promoter	NOUN
biotropia-134	244	9	from	from	ADP
biotropia-134	244	10	humpback	humpback	PROPN
biotropia-134	244	11	grouper	grouper	NOUN
biotropia-134	244	12	takeuchi	takeuchi	PROPN
biotropia-134	244	13	.	.	PUNCT
biotropia-134	245	1	2005	2005	NUM
biotropia-134	245	2	.	.	PUNCT
biotropia-134	246	1	enhancement	enhancement	NOUN
biotropia-134	246	2	of	of	ADP
biotropia-134	246	3	epa	epa	PROPN
biotropia-134	246	4	and	and	CCONJ
biotropia-134	246	5	dha	dha	PROPN
biotropia-134	246	6	biosynthesis	biosynthesis	NOUN
biotropia-134	246	7	by	by	ADP
biotropia-134	246	8	over	over	ADP
biotropia-134	246	9	-	-	PUNCT
biotropia-134	246	10	expression	expression	NOUN
biotropia-134	246	11	of	of	ADP
biotropia-134	246	12	masu	masu	PROPN
biotropia-134	246	13	salmon	salmon	PROPN
biotropia-134	246	14	δ6	δ6	PROPN
biotropia-134	246	15	-	-	PUNCT
biotropia-134	246	16	desaturase	desaturase	NOUN
biotropia-134	246	17	-	-	PUNCT
biotropia-134	246	18	like	like	ADJ
biotropia-134	246	19	gene	gene	NOUN
biotropia-134	246	20	in	in	ADP
biotropia-134	246	21	zebrafish	zebrafish	NUM
biotropia-134	246	22	.	.	PUNCT
biotropia-134	247	1	β	β	X
biotropia-134	247	2	-	-	PUNCT
biotropia-134	247	3	actin	actin	ADJ
biotropia-134	247	4	promoter	promoter	NOUN
biotropia-134	247	5	from	from	ADP
biotropia-134	247	6	japanese	japanese	PROPN
biotropia-134	247	7	medaka	medaka	PROPN
biotropia-134	247	8	oncorhynchus	oncorhynchus	PROPN
biotropia-134	247	9	masou	masou	NOUN
biotropia-134	247	10	cyprinus	cyprinus	ADP
biotropia-134	247	11	carpio	carpio	NOUN
biotropia-134	247	12	sparus	sparus	NOUN
biotropia-134	247	13	sarba	sarba	NOUN
biotropia-134	247	14	.	.	PUNCT
biotropia-134	248	1	marine	marine	PROPN
biotropia-134	248	2	biotechnology	biotechnology	PROPN
biotropia-134	248	3	oncorhynchus	oncorhynchus	PROPN
biotropia-134	248	4	tshawytsca	tshawytsca	NOUN
biotropia-134	248	5	23	23	NUM
biotropia-134	248	6	sun	sun	PROPN
biotropia-134	248	7	ps	ps	PROPN
biotropia-134	248	8	,	,	PUNCT
biotropia-134	248	9	venzonnc	venzonnc	PROPN
biotropia-134	248	10	,	,	PUNCT
biotropia-134	248	11	calderon	calderon	NOUN
biotropia-134	248	12	fro	fro	NOUN
biotropia-134	248	13	and	and	CCONJ
biotropia-134	248	14	esaki	esaki	NOUN
biotropia-134	248	15	dm	dm	PROPN
biotropia-134	248	16	.	.	PROPN
biotropia-134	248	17	2005	2005	NUM
biotropia-134	248	18	.	.	PUNCT
biotropia-134	249	1	evaluation	evaluation	NOUN
biotropia-134	249	2	of	of	ADP
biotropia-134	249	3	methods	method	NOUN
biotropia-134	249	4	for	for	ADP
biotropia-134	249	5	dna	dna	PROPN
biotropia-134	249	6	delivery	delivery	NOUN
biotropia-134	249	7	into	into	ADP
biotropia-134	249	8	shrimp	shrimp	NOUN
biotropia-134	249	9	zygotes	zygote	NOUN
biotropia-134	249	10	of	of	ADP
biotropia-134	249	11	(	(	PUNCT
biotropia-134	249	12	)	)	PUNCT
biotropia-134	249	13	.	.	PUNCT
biotropia-134	250	1	,	,	PUNCT
biotropia-134	250	2	243:19	243:19	NUM
biotropia-134	250	3	-	-	SYM
biotropia-134	250	4	26	26	NUM
biotropia-134	250	5	.	.	PUNCT
biotropia-134	251	1	symon	symon	PROPN
biotropia-134	251	2	je	je	PROPN
biotropia-134	251	3	,	,	PUNCT
biotropia-134	251	4	walker	walker	PROPN
biotropia-134	251	5	sp	sp	PROPN
biotropia-134	251	6	,	,	PUNCT
biotropia-134	251	7	sin	sin	NOUN
biotropia-134	251	8	fyt	fyt	PROPN
biotropia-134	251	9	and	and	CCONJ
biotropia-134	251	10	l.	l.	PROPN
biotropia-134	251	11	sin	sin	PROPN
biotropia-134	251	12	.	.	PUNCT
biotropia-134	252	1	1994	1994	NUM
biotropia-134	252	2	.	.	PUNCT
biotropia-134	253	1	development	development	NOUN
biotropia-134	253	2	of	of	ADP
biotropia-134	253	3	a	a	DET
biotropia-134	253	4	mass	mass	ADJ
biotropia-134	253	5	gene	gene	NOUN
biotropia-134	253	6	transfer	transfer	NOUN
biotropia-134	253	7	method	method	NOUN
biotropia-134	253	8	in	in	ADP
biotropia-134	253	9	chinook	chinook	NOUN
biotropia-134	253	10	salmon	salmon	NOUN
biotropia-134	253	11	:	:	PUNCT
biotropia-134	253	12	optimization	optimization	NOUN
biotropia-134	253	13	of	of	ADP
biotropia-134	253	14	gene	gene	NOUN
biotropia-134	253	15	transfer	transfer	NOUN
biotropia-134	253	16	by	by	ADP
biotropia-134	253	17	electroporated	electroporate	VERB
biotropia-134	253	18	sperm	sperm	NOUN
biotropia-134	253	19	.	.	PUNCT
biotropia-134	254	1	molecular	molecular	ADJ
biotropia-134	254	2	marine	marine	ADJ
biotropia-134	254	3	biology	biology	NOUN
biotropia-134	254	4	and	and	CCONJ
biotropia-134	254	5	biotechnology	biotechnology	NOUN
biotropia-134	254	6	3:104	3:104	PROPN
biotropia-134	254	7	-	-	PUNCT
biotropia-134	254	8	111	111	NUM
biotropia-134	254	9	.	.	PUNCT
biotropia-134	255	1	teitelbaum	teitelbaum	PROPN
biotropia-134	255	2	a.	a.	PROPN
biotropia-134	255	3	2007	2007	NUM
biotropia-134	255	4	.	.	PUNCT
biotropia-134	256	1	pacific	pacific	ADJ
biotropia-134	256	2	islanders	islander	NOUN
biotropia-134	256	3	gain	gain	VERB
biotropia-134	256	4	specific	specific	ADJ
biotropia-134	256	5	knowhhow	knowhhow	NOUN
biotropia-134	256	6	on	on	ADP
biotropia-134	256	7	grouper	grouper	NOUN
biotropia-134	256	8	hatchery	hatchery	NOUN
biotropia-134	256	9	techniques.http://	techniques.http://	PUNCT
biotropia-134	256	10	www.spc.int/coastfish/news/fish_news/	www.spc.int/coastfish/news/fish_news/	PROPN
biotropia-134	256	11	121	121	NUM
biotropia-134	256	12	/	/	SYM
biotropia-134	256	13	teitelbaum	teitelbaum	NOUN
biotropia-134	256	14	_	_	NOUN
biotropia-134	256	15	121.pdf	121.pdf	NUM
biotropia-134	256	16	.	.	PUNCT
biotropia-134	257	1	spc	spc	PROPN
biotropia-134	257	2	fisheries	fisheries	PROPN
biotropia-134	257	3	newsletter	newsletter	NOUN
biotropia-134	257	4	#	#	SYM
biotropia-134	257	5	121	121	NUM
biotropia-134	257	6	april	april	PROPN
biotropia-134	257	7	/	/	SYM
biotropia-134	257	8	june	june	PROPN
biotropia-134	257	9	2007	2007	NUM
biotropia-134	257	10	.	.	PUNCT
biotropia-134	258	1	tsai	tsai	PROPN
biotropia-134	258	2	hj	hj	PROPN
biotropia-134	258	3	,	,	PUNCT
biotropia-134	258	4	lai	lai	PROPN
biotropia-134	258	5	ch	ch	PROPN
biotropia-134	258	6	and	and	CCONJ
biotropia-134	258	7	hs	hs	PROPN
biotropia-134	258	8	yang	yang	PROPN
biotropia-134	258	9	.	.	PROPN
biotropia-134	258	10	1997	1997	NUM
biotropia-134	258	11	.	.	PUNCT
biotropia-134	259	1	sperm	sperm	NOUN
biotropia-134	259	2	as	as	ADP
biotropia-134	259	3	carrier	carrier	NOUN
biotropia-134	259	4	to	to	PART
biotropia-134	259	5	introduce	introduce	VERB
biotropia-134	259	6	an	an	DET
biotropia-134	259	7	exogenous	exogenous	ADJ
biotropia-134	259	8	dna	dna	NOUN
biotropia-134	259	9	fragment	fragment	NOUN
biotropia-134	259	10	into	into	ADP
biotropia-134	259	11	the	the	DET
biotropia-134	259	12	oocyte	oocyte	NOUN
biotropia-134	259	13	of	of	ADP
biotropia-134	259	14	japanesse	japanesse	PROPN
biotropia-134	259	15	abalone	abalone	PROPN
biotropia-134	259	16	(	(	PUNCT
biotropia-134	259	17	)	)	PUNCT
biotropia-134	259	18	.	.	PUNCT
biotropia-134	260	1	transgenic	transgenic	NOUN
biotropia-134	260	2	research	research	NOUN
biotropia-134	260	3	,	,	PUNCT
biotropia-134	260	4	6:85	6:85	NUM
biotropia-134	260	5	-	-	SYM
biotropia-134	260	6	95	95	NUM
biotropia-134	260	7	.	.	PUNCT
biotropia-134	261	1	tucker	tucker	PROPN
biotropia-134	261	2	jr	jr	PROPN
biotropia-134	261	3	.	.	PROPN
biotropia-134	261	4	and	and	CCONJ
biotropia-134	261	5	w	w	PROPN
biotropia-134	261	6	john	john	PROPN
biotropia-134	261	7	.	.	PROPN
biotropia-134	261	8	1999	1999	NUM
biotropia-134	261	9	.	.	PUNCT
biotropia-134	262	1	species	specie	NOUN
biotropia-134	262	2	profile	profile	VERB
biotropia-134	262	3	grouper	grouper	NOUN
biotropia-134	262	4	aquaculture	aquaculture	NOUN
biotropia-134	262	5	.	.	PUNCT
biotropia-134	263	1	srac	srac	PROPN
biotropia-134	263	2	publication	publication	NOUN
biotropia-134	264	1	no	no	INTJ
biotropia-134	264	2	.	.	PROPN
biotropia-134	265	1	721	721	NUM
biotropia-134	265	2	.	.	PUNCT
biotropia-134	266	1	http://www.ca.uky.edu/wkrec/	http://www.ca.uky.edu/wkrec/	PROPN
biotropia-134	266	2	grouperaquaculture.pdf	grouperaquaculture.pdf	PROPN
biotropia-134	266	3	.	.	PUNCT
biotropia-134	266	4	penaeus	penaeus	PROPN
biotropia-134	266	5	litopenaeus	litopenaeus	PROPN
biotropia-134	266	6	vannamei	vannamei	PROPN
biotropia-134	266	7	aquaculture	aquaculture	NOUN
biotropia-134	266	8	,	,	PUNCT
biotropia-134	266	9	haliotis	haliotis	ADP
biotropia-134	266	10	deversicolor	deversicolor	NOUN
biotropia-134	266	11	supertexta	supertexta	NOUN
biotropia-134	266	12	gene	gene	NOUN
biotropia-134	266	13	transfer	transfer	NOUN
biotropia-134	266	14	method	method	NOUN
biotropia-134	266	15	in	in	ADP
biotropia-134	266	16	humpback	humpback	NOUN
biotropia-134	266	17	grouper	grouper	NOUN
biotropia-134	266	18	alimuddin	alimuddin	PROPN
biotropia-134	266	19	.et	.et	PUNCT
biotropia-134	267	1	al	al	PROPN