id sid tid token lemma pos biotropia-163 1 1 biotropia biotropia NOUN biotropia-163 1 2 no no DET biotropia-163 1 3 biotropia biotropia ADJ biotropia-163 1 4 no no NOUN biotropia-163 1 5 . . PROPN biotropia-163 1 6 16 16 NUM biotropia-163 1 7 , , PUNCT biotropia-163 1 8 2001 2001 NUM biotropia-163 1 9 : : PUNCT biotropia-163 1 10 10 10 NUM biotropia-163 1 11 -17 -17 PUNCT biotropia-163 1 12 a a DET biotropia-163 1 13 novel novel ADJ biotropia-163 1 14 integron integron NOUN biotropia-163 1 15 in in ADP biotropia-163 1 16 the the DET biotropia-163 1 17 genome genome NOUN biotropia-163 1 18 of of ADP biotropia-163 1 19 escherichia escherichia NOUN biotropia-163 1 20 coli coli NOUN biotropia-163 1 21 isolated isolate VERB biotropia-163 1 22 from from ADP biotropia-163 1 23 indonesian indonesian PROPN biotropia-163 1 24 monitor monitor PROPN biotropia-163 1 25 lizard lizard NOUN biotropia-163 1 26 ( ( PUNCT biotropia-163 1 27 varanus varanus NOUN biotropia-163 1 28 spp spp NOUN biotropia-163 1 29 ) ) PUNCT biotropia-163 1 30 . . PUNCT biotropia-163 2 1 esti esti PROPN biotropia-163 2 2 puspitasari2 puspitasari2 PROPN biotropia-163 2 3 , , PUNCT biotropia-163 2 4 antonius antonius PROPN biotropia-163 2 5 suwanto1'2 suwanto1'2 NOUN biotropia-163 2 6 * * PUNCT biotropia-163 2 7 , , PUNCT biotropia-163 2 8 amarila amarila NOUN biotropia-163 2 9 malik3 malik3 PROPN biotropia-163 2 10 , , PUNCT biotropia-163 2 11 and and CCONJ biotropia-163 2 12 walter walter PROPN biotropia-163 2 13 erdelen erdelen VERB biotropia-163 2 14 " " PUNCT biotropia-163 2 15 ' ' PUNCT biotropia-163 2 16 dept dept NOUN biotropia-163 2 17 . . PROPN biotropia-163 2 18 of of ADP biotropia-163 2 19 biology biology NOUN biotropia-163 2 20 , , PUNCT biotropia-163 2 21 faculty faculty NOUN biotropia-163 2 22 of of ADP biotropia-163 2 23 science science NOUN biotropia-163 2 24 and and CCONJ biotropia-163 2 25 mathematics mathematic NOUN biotropia-163 2 26 and and CCONJ biotropia-163 2 27 ivc ivc PROPN biotropia-163 2 28 biotechnology biotechnology NOUN biotropia-163 2 29 , , PUNCT biotropia-163 2 30 bogor bogor NOUN biotropia-163 2 31 , , PUNCT biotropia-163 2 32 agricultural agricultural ADJ biotropia-163 2 33 university university NOUN biotropia-163 2 34 , , PUNCT biotropia-163 2 35 bogor bogor PROPN biotropia-163 2 36 , , PUNCT biotropia-163 2 37 indonesia indonesia PROPN biotropia-163 2 38 ; ; PUNCT biotropia-163 2 39 2 2 NUM biotropia-163 2 40 south south PROPN biotropia-163 2 41 east east PROPN biotropia-163 2 42 asian asian ADJ biotropia-163 2 43 regional regional ADJ biotropia-163 2 44 center center NOUN biotropia-163 2 45 for for ADP biotropia-163 2 46 tropical tropical ADJ biotropia-163 2 47 biology biology NOUN biotropia-163 2 48 ( ( PUNCT biotropia-163 2 49 seameo seameo NOUN biotropia-163 2 50 biotrop biotrop NOUN biotropia-163 2 51 ) ) PUNCT biotropia-163 2 52 , , PUNCT biotropia-163 2 53 bogor bogor PROPN biotropia-163 2 54 : : PUNCT biotropia-163 2 55 indonesia indonesia PROPN biotropia-163 2 56 3 3 NUM biotropia-163 2 57 dept dept PROPN biotropia-163 2 58 . . PROPN biotropia-163 2 59 of of ADP biotropia-163 2 60 pharmacy pharmacy NOUN biotropia-163 2 61 , , PUNCT biotropia-163 2 62 faculty faculty NOUN biotropia-163 2 63 of of ADP biotropia-163 2 64 science science NOUN biotropia-163 2 65 and and CCONJ biotropia-163 2 66 mathematics mathematic NOUN biotropia-163 2 67 , , PUNCT biotropia-163 2 68 university university PROPN biotropia-163 2 69 of of ADP biotropia-163 2 70 indonesia indonesia PROPN biotropia-163 2 71 , , PUNCT biotropia-163 2 72 jakarta jakarta PROPN biotropia-163 2 73 , , PUNCT biotropia-163 2 74 indonesia indonesia PROPN biotropia-163 2 75 ; ; PUNCT biotropia-163 2 76 ' ' PUNCT biotropia-163 2 77 dept dept NOUN biotropia-163 2 78 . . PROPN biotropia-163 2 79 of of ADP biotropia-163 2 80 biology biology NOUN biotropia-163 2 81 , , PUNCT biotropia-163 2 82 faculty faculty NOUN biotropia-163 2 83 of of ADP biotropia-163 2 84 science science NOUN biotropia-163 2 85 and and CCONJ biotropia-163 2 86 mathematics mathematic NOUN biotropia-163 2 87 , , PUNCT biotropia-163 2 88 bandung bandung PROPN biotropia-163 2 89 institute institute PROPN biotropia-163 2 90 of of ADP biotropia-163 2 91 technology technology PROPN biotropia-163 2 92 , , PUNCT biotropia-163 2 93 bandung bandung PROPN biotropia-163 2 94 , , PUNCT biotropia-163 2 95 indonesia indonesia PROPN biotropia-163 2 96 abstract abstract VERB biotropia-163 2 97 the the DET biotropia-163 2 98 genotype genotype NOUN biotropia-163 2 99 of of ADP biotropia-163 2 100 antibiotic antibiotic ADJ biotropia-163 2 101 resistance resistance NOUN biotropia-163 2 102 in in ADP biotropia-163 2 103 natural natural ADJ biotropia-163 2 104 isolates isolate NOUN biotropia-163 2 105 of of ADP biotropia-163 2 106 escherichia escherichia NOUN biotropia-163 2 107 coli coli NOUN biotropia-163 2 108 was be AUX biotropia-163 2 109 determined determine VERB biotropia-163 2 110 through through ADP biotropia-163 2 111 integron integron NOUN biotropia-163 2 112 detection detection NOUN biotropia-163 2 113 and and CCONJ biotropia-163 2 114 characterization characterization NOUN biotropia-163 2 115 of of ADP biotropia-163 2 116 the the DET biotropia-163 2 117 associated associated ADJ biotropia-163 2 118 antibiotic antibiotic ADJ biotropia-163 2 119 resistance resistance NOUN biotropia-163 2 120 . . PUNCT biotropia-163 3 1 e. e. PROPN biotropia-163 3 2 coli coli PROPN biotropia-163 3 3 sg2 sg2 PROPN biotropia-163 3 4 isolated isolate VERB biotropia-163 3 5 from from ADP biotropia-163 3 6 varanus varanus PROPN biotropia-163 3 7 salvator salvator NOUN biotropia-163 3 8 of of ADP biotropia-163 3 9 java java PROPN biotropia-163 3 10 demonstrated demonstrate VERB biotropia-163 3 11 resistance resistance NOUN biotropia-163 3 12 to to ADP biotropia-163 3 13 spectinomycin spectinomycin PROPN biotropia-163 3 14 ( ( PUNCT biotropia-163 3 15 50ng 50ng NUM biotropia-163 3 16 / / SYM biotropia-163 3 17 ml ml NOUN biotropia-163 3 18 ) ) PUNCT biotropia-163 3 19 and and CCONJ biotropia-163 3 20 streptomycin streptomycin PROPN biotropia-163 3 21 ( ( PUNCT biotropia-163 3 22 song song PROPN biotropia-163 3 23 / / SYM biotropia-163 3 24 ml ml NOUN biotropia-163 3 25 ) ) PUNCT biotropia-163 3 26 . . PUNCT biotropia-163 4 1 integron integron PROPN biotropia-163 4 2 detection detection PROPN biotropia-163 4 3 indicated indicate VERB biotropia-163 4 4 that that SCONJ biotropia-163 4 5 eight eight NUM biotropia-163 4 6 isolates isolate NOUN biotropia-163 4 7 out out ADP biotropia-163 4 8 of of ADP biotropia-163 4 9 nine nine NUM biotropia-163 4 10 e. e. PROPN biotropia-163 4 11 coli coli NOUN biotropia-163 4 12 isolates isolate NOUN biotropia-163 4 13 possessed possess VERB biotropia-163 4 14 a a DET biotropia-163 4 15 conserved conserved ADJ biotropia-163 4 16 segment segment NOUN biotropia-163 4 17 of of ADP biotropia-163 4 18 the the DET biotropia-163 4 19 integron integron PROPN biotropia-163 4 20 . . PUNCT biotropia-163 5 1 amplification amplification NOUN biotropia-163 5 2 of of ADP biotropia-163 5 3 the the DET biotropia-163 5 4 inserted insert VERB biotropia-163 5 5 cassette cassette NOUN biotropia-163 5 6 of of ADP biotropia-163 5 7 the the DET biotropia-163 5 8 integron integron NOUN biotropia-163 5 9 in in ADP biotropia-163 5 10 this this DET biotropia-163 5 11 sg2 sg2 PROPN biotropia-163 5 12 isolate isolate PROPN biotropia-163 5 13 yielded yield VERB biotropia-163 5 14 a a DET biotropia-163 5 15 1 1 NUM biotropia-163 5 16 - - PUNCT biotropia-163 5 17 kb kb PROPN biotropia-163 5 18 dna dna PROPN biotropia-163 5 19 fragment fragment NOUN biotropia-163 5 20 . . PUNCT biotropia-163 6 1 sequence sequence NOUN biotropia-163 6 2 analyses analysis NOUN biotropia-163 6 3 indicated indicate VERB biotropia-163 6 4 that that SCONJ biotropia-163 6 5 this this DET biotropia-163 6 6 fragment fragment NOUN biotropia-163 6 7 was be AUX biotropia-163 6 8 homologous homologous ADJ biotropia-163 6 9 with with ADP biotropia-163 6 10 aad aad PROPN biotropia-163 6 11 gene gene PROPN biotropia-163 6 12 , , PUNCT biotropia-163 6 13 which which PRON biotropia-163 6 14 confirmed confirm VERB biotropia-163 6 15 the the DET biotropia-163 6 16 resistance resistance NOUN biotropia-163 6 17 to to ADP biotropia-163 6 18 spectinomycin spectinomycin PROPN biotropia-163 6 19 / / SYM biotropia-163 6 20 streptomycin streptomycin PROPN biotropia-163 6 21 . . PUNCT biotropia-163 7 1 this this PRON biotropia-163 7 2 is be AUX biotropia-163 7 3 the the DET biotropia-163 7 4 first first ADJ biotropia-163 7 5 report report NOUN biotropia-163 7 6 on on ADP biotropia-163 7 7 the the DET biotropia-163 7 8 presence presence NOUN biotropia-163 7 9 of of ADP biotropia-163 7 10 integron integron NOUN biotropia-163 7 11 in in ADP biotropia-163 7 12 the the DET biotropia-163 7 13 e. e. PROPN biotropia-163 7 14 coli coli PROPN biotropia-163 7 15 isolated isolate VERB biotropia-163 7 16 from from ADP biotropia-163 7 17 the the DET biotropia-163 7 18 environment environment NOUN biotropia-163 7 19 . . PUNCT biotropia-163 8 1 key key ADJ biotropia-163 8 2 words word NOUN biotropia-163 8 3 : : PUNCT biotropia-163 8 4 integron integron PROPN biotropia-163 8 5 / / SYM biotropia-163 8 6 antibiotic antibiotic ADJ biotropia-163 8 7 resistance resistance NOUN biotropia-163 8 8 / / SYM biotropia-163 8 9 escherichia escherichia NOUN biotropia-163 8 10 coli coli NOUN biotropia-163 8 11 introduction introduction NOUN biotropia-163 8 12 antibiotic antibiotic NOUN biotropia-163 8 13 is be AUX biotropia-163 8 14 a a DET biotropia-163 8 15 substance substance NOUN biotropia-163 8 16 produced produce VERB biotropia-163 8 17 by by ADP biotropia-163 8 18 a a DET biotropia-163 8 19 microorganism microorganism NOUN biotropia-163 8 20 as as ADP biotropia-163 8 21 protection protection NOUN biotropia-163 8 22 from from ADP biotropia-163 8 23 another another DET biotropia-163 8 24 microorganism microorganism NOUN biotropia-163 8 25 ( ( PUNCT biotropia-163 8 26 madigan madigan PROPN biotropia-163 8 27 et et PROPN biotropia-163 8 28 al al PROPN biotropia-163 8 29 . . PROPN biotropia-163 8 30 1997 1997 NUM biotropia-163 8 31 ) ) PUNCT biotropia-163 8 32 . . PUNCT biotropia-163 9 1 antibiotics antibiotic NOUN biotropia-163 9 2 have have VERB biotropia-163 9 3 a a DET biotropia-163 9 4 specific specific ADJ biotropia-163 9 5 target target NOUN biotropia-163 9 6 site site NOUN biotropia-163 9 7 in in ADP biotropia-163 9 8 the the DET biotropia-163 9 9 cell cell NOUN biotropia-163 9 10 . . PUNCT biotropia-163 10 1 for for ADP biotropia-163 10 2 example example NOUN biotropia-163 10 3 , , PUNCT biotropia-163 10 4 in in ADP biotropia-163 10 5 trying try VERB biotropia-163 10 6 to to PART biotropia-163 10 7 get get VERB biotropia-163 10 8 hold hold NOUN biotropia-163 10 9 of of ADP biotropia-163 10 10 protein protein NOUN biotropia-163 10 11 synthesis synthesis NOUN biotropia-163 10 12 targeted target VERB biotropia-163 10 13 to to ADP biotropia-163 10 14 30s 30 NOUN biotropia-163 10 15 ribosom ribosom VERB biotropia-163 10 16 , , PUNCT biotropia-163 10 17 the the DET biotropia-163 10 18 presence presence NOUN biotropia-163 10 19 of of ADP biotropia-163 10 20 tetracycline tetracycline NOUN biotropia-163 10 21 , , PUNCT biotropia-163 10 22 spectinomycin spectinomycin PROPN biotropia-163 10 23 and and CCONJ biotropia-163 10 24 streptomycin streptomycin PROPN biotropia-163 10 25 results result VERB biotropia-163 10 26 in in ADP biotropia-163 10 27 an an DET biotropia-163 10 28 invalid invalid ADJ biotropia-163 10 29 codon codon NOUN biotropia-163 10 30 - - PUNCT biotropia-163 10 31 anticodon anticodon NOUN biotropia-163 10 32 reaction reaction NOUN biotropia-163 10 33 and and CCONJ biotropia-163 10 34 therefore therefore ADV biotropia-163 10 35 , , PUNCT biotropia-163 10 36 the the DET biotropia-163 10 37 synthesized synthesize VERB biotropia-163 10 38 amino amino NOUN biotropia-163 10 39 acid acid NOUN biotropia-163 10 40 is be AUX biotropia-163 10 41 also also ADV biotropia-163 10 42 invalid invalid ADJ biotropia-163 10 43 ( ( PUNCT biotropia-163 10 44 schumm schumm PROPN biotropia-163 10 45 1992 1992 NUM biotropia-163 10 46 ) ) PUNCT biotropia-163 10 47 . . PUNCT biotropia-163 11 1 currently currently ADV biotropia-163 11 2 , , PUNCT biotropia-163 11 3 there there PRON biotropia-163 11 4 is be VERB biotropia-163 11 5 concern concern NOUN biotropia-163 11 6 on on ADP biotropia-163 11 7 the the DET biotropia-163 11 8 spread spread NOUN biotropia-163 11 9 of of ADP biotropia-163 11 10 antibiotic antibiotic ADJ biotropia-163 11 11 resistance resistance NOUN biotropia-163 11 12 among among ADP biotropia-163 11 13 bacteria bacteria NOUN biotropia-163 11 14 . . PUNCT biotropia-163 12 1 such such ADJ biotropia-163 12 2 dissemination dissemination NOUN biotropia-163 12 3 is be AUX biotropia-163 12 4 partly partly ADV biotropia-163 12 5 a a DET biotropia-163 12 6 consequence consequence NOUN biotropia-163 12 7 of of ADP biotropia-163 12 8 the the DET biotropia-163 12 9 antibiotic antibiotic ADJ biotropia-163 12 10 - - PUNCT biotropia-163 12 11 resistant resistant ADJ biotropia-163 12 12 genes gene NOUN biotropia-163 12 13 mobility mobility NOUN biotropia-163 12 14 which which PRON biotropia-163 12 15 reside reside VERB biotropia-163 12 16 in in ADP biotropia-163 12 17 mobile mobile ADJ biotropia-163 12 18 genetic genetic ADJ biotropia-163 12 19 elements element NOUN biotropia-163 12 20 such such ADJ biotropia-163 12 21 as as ADP biotropia-163 12 22 plasmids plasmid NOUN biotropia-163 12 23 , , PUNCT biotropia-163 12 24 transposons transposon NOUN biotropia-163 12 25 , , PUNCT biotropia-163 12 26 and and CCONJ biotropia-163 12 27 integrons integron NOUN biotropia-163 12 28 ( ( PUNCT biotropia-163 12 29 francia francia PROPN biotropia-163 12 30 et et PROPN biotropia-163 12 31 al al PROPN biotropia-163 12 32 . . PROPN biotropia-163 12 33 1999 1999 NUM biotropia-163 12 34 ) ) PUNCT biotropia-163 12 35 . . PUNCT biotropia-163 13 1 integron integron PROPN biotropia-163 13 2 is be AUX biotropia-163 13 3 a a DET biotropia-163 13 4 new new ADJ biotropia-163 13 5 type type NOUN biotropia-163 13 6 of of ADP biotropia-163 13 7 mobile mobile ADJ biotropia-163 13 8 element element NOUN biotropia-163 13 9 which which PRON biotropia-163 13 10 has have AUX biotropia-163 13 11 evolved evolve VERB biotropia-163 13 12 by by ADP biotropia-163 13 13 a a DET biotropia-163 13 14 site site NOUN biotropia-163 13 15 - - PUNCT biotropia-163 13 16 specific specific ADJ biotropia-163 13 17 recombination recombination NOUN biotropia-163 13 18 mechanism mechanism NOUN biotropia-163 13 19 . . PUNCT biotropia-163 14 1 integrons integron NOUN biotropia-163 14 2 consist consist VERB biotropia-163 14 3 of of ADP biotropia-163 14 4 two two NUM biotropia-163 14 5 conserved conserved ADJ biotropia-163 14 6 segments segment NOUN biotropia-163 14 7 of of ADP biotropia-163 14 8 dna dna PROPN biotropia-163 14 9 separated separate VERB biotropia-163 14 10 by by ADP biotropia-163 14 11 a a DET biotropia-163 14 12 variable variable ADJ biotropia-163 14 13 region region NOUN biotropia-163 14 14 containing contain VERB biotropia-163 14 15 one one NUM biotropia-163 14 16 or or CCONJ biotropia-163 14 17 more more ADJ biotropia-163 14 18 genes gene NOUN biotropia-163 14 19 integrated integrate VERB biotropia-163 14 20 as as ADP biotropia-163 14 21 cassettes cassette NOUN biotropia-163 14 22 ( ( PUNCT biotropia-163 14 23 levesque levesque PROPN biotropia-163 14 24 et et PROPN biotropia-163 14 25 al al PROPN biotropia-163 14 26 . . PROPN biotropia-163 14 27 1995 1995 NUM biotropia-163 14 28 ) ) PUNCT biotropia-163 14 29 . . PUNCT biotropia-163 15 1 the the DET biotropia-163 15 2 5'conserved 5'conserved NUM biotropia-163 15 3 segment segment NOUN biotropia-163 15 4 contained contain VERB biotropia-163 15 5 in in ADP biotropia-163 15 6 the the DET biotropia-163 15 7 int int NOUN biotropia-163 15 8 gene gene NOUN biotropia-163 15 9 , , PUNCT biotropia-163 15 10 which which PRON biotropia-163 15 11 encodes encode VERB biotropia-163 15 12 a a DET biotropia-163 15 13 polypeptide polypeptide NOUN biotropia-163 15 14 of of ADP biotropia-163 15 15 337 337 NUM biotropia-163 15 16 amino amino NOUN biotropia-163 15 17 acids acid NOUN biotropia-163 15 18 , , PUNCT biotropia-163 15 19 has have AUX biotropia-163 15 20 been be AUX biotropia-163 15 21 shown show VERB biotropia-163 15 22 to to PART biotropia-163 15 23 be be AUX biotropia-163 15 24 homologous homologous ADJ biotropia-163 15 25 to to ADP biotropia-163 15 26 other other ADJ biotropia-163 15 27 members member NOUN biotropia-163 15 28 of of ADP biotropia-163 15 29 the the DET biotropia-163 15 30 integrase integrase NOUN biotropia-163 15 31 family family NOUN biotropia-163 15 32 and and CCONJ biotropia-163 15 33 on on ADP biotropia-163 15 34 the the DET biotropia-163 15 35 opposite opposite ADJ biotropia-163 15 36 strand strand NOUN biotropia-163 15 37 , , PUNCT biotropia-163 15 38 a a DET biotropia-163 15 39 common common ADJ biotropia-163 15 40 promoter promoter NOUN biotropia-163 15 41 region region NOUN biotropia-163 15 42 p1 p1 PROPN biotropia-163 15 43 - - PUNCT biotropia-163 15 44 p2 p2 PROPN biotropia-163 15 45 is be AUX biotropia-163 15 46 directed direct VERB biotropia-163 15 47 toward toward ADP biotropia-163 15 48 the the DET biotropia-163 15 49 site site NOUN biotropia-163 15 50 of of ADP biotropia-163 15 51 integration integration NOUN biotropia-163 15 52 . . PUNCT biotropia-163 16 1 the the DET biotropia-163 16 2 3 3 NUM biotropia-163 16 3 ' ' PART biotropia-163 16 4 conserved conserved ADJ biotropia-163 16 5 segment segment NOUN biotropia-163 16 6 contains contain VERB biotropia-163 16 7 the the DET biotropia-163 16 8 qace&\ qace&\ PROPN biotropia-163 16 9 , , PUNCT biotropia-163 16 10 sur sur NOUN biotropia-163 16 11 genes gene NOUN biotropia-163 16 12 , , PUNCT biotropia-163 16 13 and and CCONJ biotropia-163 16 14 an an DET biotropia-163 16 15 open open ADJ biotropia-163 16 16 reading reading NOUN biotropia-163 16 17 frame frame NOUN biotropia-163 16 18 ( ( PUNCT biotropia-163 16 19 fig.l fig.l PROPN biotropia-163 16 20 ) ) PUNCT biotropia-163 16 21 . . PUNCT biotropia-163 17 1 * * PUNCT biotropia-163 17 2 corresponding correspond VERB biotropia-163 17 3 author author NOUN biotropia-163 17 4 : : PUNCT biotropia-163 17 5 e e X biotropia-163 17 6 - - NOUN biotropia-163 17 7 mail mail NOUN biotropia-163 17 8 address address NOUN biotropia-163 17 9 : : PUNCT biotropia-163 17 10 asuwanto@indo.net.id asuwanto@indo.net.id NOUN biotropia-163 17 11 10 10 NUM biotropia-163 17 12 10 10 NUM biotropia-163 17 13 figure figure NOUN biotropia-163 17 14 1 1 NUM biotropia-163 17 15 . . PUNCT biotropia-163 17 16 integron integron PROPN biotropia-163 17 17 structure structure NOUN biotropia-163 17 18 ( ( PUNCT biotropia-163 17 19 levesque levesque PROPN biotropia-163 17 20 et et PROPN biotropia-163 17 21 al al PROPN biotropia-163 17 22 . . PROPN biotropia-163 17 23 1995 1995 NUM biotropia-163 17 24 ) ) PUNCT biotropia-163 17 25 genetic genetic ADJ biotropia-163 17 26 changes change NOUN biotropia-163 17 27 in in ADP biotropia-163 17 28 bacterial bacterial ADJ biotropia-163 17 29 genome genome NOUN biotropia-163 17 30 could could AUX biotropia-163 17 31 contribute contribute VERB biotropia-163 17 32 to to ADP biotropia-163 17 33 antibiotic antibiotic ADJ biotropia-163 17 34 resistance resistance NOUN biotropia-163 17 35 in in ADP biotropia-163 17 36 some some DET biotropia-163 17 37 bacteria bacteria NOUN biotropia-163 17 38 . . PUNCT biotropia-163 18 1 there there PRON biotropia-163 18 2 are be VERB biotropia-163 18 3 three three NUM biotropia-163 18 4 mechanisms mechanism NOUN biotropia-163 18 5 of of ADP biotropia-163 18 6 antibiotic antibiotic ADJ biotropia-163 18 7 inactivation inactivation NOUN biotropia-163 18 8 : : PUNCT biotropia-163 18 9 ( ( PUNCT biotropia-163 18 10 i i NOUN biotropia-163 18 11 ) ) PUNCT biotropia-163 18 12 modification modification NOUN biotropia-163 18 13 of of ADP biotropia-163 18 14 antibiotic antibiotic ADJ biotropia-163 18 15 structure structure NOUN biotropia-163 18 16 ; ; PUNCT biotropia-163 18 17 ( ( PUNCT biotropia-163 18 18 ii ii NOUN biotropia-163 18 19 ) ) PUNCT biotropia-163 18 20 prevention prevention NOUN biotropia-163 18 21 of of ADP biotropia-163 18 22 antibiotic antibiotic NOUN biotropia-163 18 23 to to PART biotropia-163 18 24 get get VERB biotropia-163 18 25 into into ADP biotropia-163 18 26 the the DET biotropia-163 18 27 target target NOUN biotropia-163 18 28 site site NOUN biotropia-163 18 29 ; ; PUNCT biotropia-163 18 30 or or CCONJ biotropia-163 18 31 ( ( PUNCT biotropia-163 18 32 iii iii NOUN biotropia-163 18 33 ) ) PUNCT biotropia-163 18 34 change change NOUN biotropia-163 18 35 in in ADP biotropia-163 18 36 antibiotic antibiotic ADJ biotropia-163 18 37 target target NOUN biotropia-163 18 38 site site NOUN biotropia-163 18 39 ( ( PUNCT biotropia-163 18 40 neu neu PROPN biotropia-163 18 41 1992 1992 NUM biotropia-163 18 42 ) ) PUNCT biotropia-163 18 43 . . PUNCT biotropia-163 19 1 resistance resistance NOUN biotropia-163 19 2 to to ADP biotropia-163 19 3 aminoglycoside aminoglycoside VERB biotropia-163 19 4 antibiotics antibiotic NOUN biotropia-163 19 5 such such ADJ biotropia-163 19 6 as as ADP biotropia-163 19 7 spectinomycin spectinomycin PROPN biotropia-163 19 8 and and CCONJ biotropia-163 19 9 streptomycin streptomycin PROPN biotropia-163 19 10 , , PUNCT biotropia-163 19 11 could could AUX biotropia-163 19 12 be be AUX biotropia-163 19 13 due due ADJ biotropia-163 19 14 to to ADP biotropia-163 19 15 the the DET biotropia-163 19 16 penetration penetration NOUN biotropia-163 19 17 failure failure NOUN biotropia-163 19 18 , , PUNCT biotropia-163 19 19 the the DET biotropia-163 19 20 low low ADJ biotropia-163 19 21 affinity affinity NOUN biotropia-163 19 22 of of ADP biotropia-163 19 23 antibiotic antibiotic ADJ biotropia-163 19 24 to to PART biotropia-163 19 25 ribosom ribosom VERB biotropia-163 19 26 , , PUNCT biotropia-163 19 27 or or CCONJ biotropia-163 19 28 the the DET biotropia-163 19 29 inactivation inactivation NOUN biotropia-163 19 30 of of ADP biotropia-163 19 31 the the DET biotropia-163 19 32 antibiotic antibiotic NOUN biotropia-163 19 33 by by ADP biotropia-163 19 34 enzymes enzyme NOUN biotropia-163 19 35 such such ADJ biotropia-163 19 36 as as ADP biotropia-163 19 37 phosphorylase phosphorylase NOUN biotropia-163 19 38 , , PUNCT biotropia-163 19 39 adenylase adenylase NOUN biotropia-163 19 40 and and CCONJ biotropia-163 19 41 acetylase acetylase NOUN biotropia-163 19 42 produced produce VERB biotropia-163 19 43 by by ADP biotropia-163 19 44 the the DET biotropia-163 19 45 bacteria bacteria NOUN biotropia-163 19 46 ( ( PUNCT biotropia-163 19 47 gian gian NOUN biotropia-163 19 48 and and CCONJ biotropia-163 19 49 gian gian NOUN biotropia-163 19 50 1995 1995 NUM biotropia-163 19 51 ) ) PUNCT biotropia-163 19 52 . . PUNCT biotropia-163 20 1 desselberger desselberger NOUN biotropia-163 20 2 ( ( PUNCT biotropia-163 20 3 1998 1998 NUM biotropia-163 20 4 ) ) PUNCT biotropia-163 20 5 reported report VERB biotropia-163 20 6 that that SCONJ biotropia-163 20 7 data datum NOUN biotropia-163 20 8 on on ADP biotropia-163 20 9 antibiotic antibiotic ADJ biotropia-163 20 10 - - PUNCT biotropia-163 20 11 resistant resistant ADJ biotropia-163 20 12 genes gene NOUN biotropia-163 20 13 in in ADP biotropia-163 20 14 the the DET biotropia-163 20 15 environment environment NOUN biotropia-163 20 16 were be AUX biotropia-163 20 17 rare rare ADJ biotropia-163 20 18 , , PUNCT biotropia-163 20 19 whereas whereas SCONJ biotropia-163 20 20 antibiotic antibiotic ADJ biotropia-163 20 21 - - PUNCT biotropia-163 20 22 resistant resistant ADJ biotropia-163 20 23 genes gene NOUN biotropia-163 20 24 among among ADP biotropia-163 20 25 bacterial bacterial ADJ biotropia-163 20 26 strains strain NOUN biotropia-163 20 27 were be AUX biotropia-163 20 28 increasing increase VERB biotropia-163 20 29 at at ADP biotropia-163 20 30 an an DET biotropia-163 20 31 alarming alarming ADJ biotropia-163 20 32 rate rate NOUN biotropia-163 20 33 . . PUNCT biotropia-163 21 1 biawak biawak ADJ biotropia-163 21 2 monitors monitor NOUN biotropia-163 21 3 ( ( PUNCT biotropia-163 21 4 varanus varanus NOUN biotropia-163 21 5 spp spp NOUN biotropia-163 21 6 . . PUNCT biotropia-163 21 7 ) ) PUNCT biotropia-163 21 8 are be AUX biotropia-163 21 9 reptilians reptilians PROPN biotropia-163 21 10 lacking lack VERB biotropia-163 21 11 venomous venomous ADJ biotropia-163 21 12 gland gland NOUN biotropia-163 21 13 , , PUNCT biotropia-163 21 14 although although SCONJ biotropia-163 21 15 there there PRON biotropia-163 21 16 are be VERB biotropia-163 21 17 some some DET biotropia-163 21 18 reports report NOUN biotropia-163 21 19 on on ADP biotropia-163 21 20 biawak biawak ADJ biotropia-163 21 21 bites bite NOUN biotropia-163 21 22 resulting result VERB biotropia-163 21 23 in in ADP biotropia-163 21 24 bacterial bacterial ADJ biotropia-163 21 25 infection infection NOUN biotropia-163 21 26 ( ( PUNCT biotropia-163 21 27 auffenberg auffenberg PROPN biotropia-163 21 28 1981 1981 NUM biotropia-163 21 29 ) ) PUNCT biotropia-163 21 30 . . PUNCT biotropia-163 22 1 probably probably ADV biotropia-163 22 2 , , PUNCT biotropia-163 22 3 this this DET biotropia-163 22 4 infection infection NOUN biotropia-163 22 5 is be AUX biotropia-163 22 6 caused cause VERB biotropia-163 22 7 by by ADP biotropia-163 22 8 toxic toxic ADJ biotropia-163 22 9 substances substance NOUN biotropia-163 22 10 produced produce VERB biotropia-163 22 11 by by ADP biotropia-163 22 12 microbiota microbiota NOUN biotropia-163 22 13 living live VERB biotropia-163 22 14 in in ADP biotropia-163 22 15 the the DET biotropia-163 22 16 oral oral NOUN biotropia-163 22 17 of of ADP biotropia-163 22 18 varanus varanus PROPN biotropia-163 22 19 spp spp PROPN biotropia-163 22 20 . . PUNCT biotropia-163 23 1 auffenberg auffenberg PROPN biotropia-163 23 2 ( ( PUNCT biotropia-163 23 3 1981 1981 NUM biotropia-163 23 4 ) ) PUNCT biotropia-163 23 5 identified identify VERB biotropia-163 23 6 five five NUM biotropia-163 23 7 bacteria bacteria NOUN biotropia-163 23 8 species specie NOUN biotropia-163 23 9 that that PRON biotropia-163 23 10 could could AUX biotropia-163 23 11 cause cause VERB biotropia-163 23 12 infection infection NOUN biotropia-163 23 13 . . PUNCT biotropia-163 24 1 yogiara yogiara NOUN biotropia-163 24 2 ( ( PUNCT biotropia-163 24 3 1998 1998 NUM biotropia-163 24 4 ) ) PUNCT biotropia-163 24 5 reported report VERB biotropia-163 24 6 that that SCONJ biotropia-163 24 7 many many ADJ biotropia-163 24 8 of of ADP biotropia-163 24 9 e. e. PROPN biotropia-163 24 10 coli coli PROPN biotropia-163 24 11 isolates isolate NOUN biotropia-163 24 12 living live VERB biotropia-163 24 13 in in ADP biotropia-163 24 14 the the DET biotropia-163 24 15 buccal buccal ADJ biotropia-163 24 16 of of ADP biotropia-163 24 17 varanus varanus PROPN biotropia-163 24 18 spp spp PROPN biotropia-163 24 19 . . PUNCT biotropia-163 24 20 are be AUX biotropia-163 24 21 resistant resistant ADJ biotropia-163 24 22 to to ADP biotropia-163 24 23 ampicillin ampicillin PROPN biotropia-163 24 24 . . PUNCT biotropia-163 25 1 this this DET biotropia-163 25 2 study study NOUN biotropia-163 25 3 was be AUX biotropia-163 25 4 conducted conduct VERB biotropia-163 25 5 in in ADP biotropia-163 25 6 order order NOUN biotropia-163 25 7 to to PART biotropia-163 25 8 understand understand VERB biotropia-163 25 9 the the DET biotropia-163 25 10 mechanism mechanism NOUN biotropia-163 25 11 of of ADP biotropia-163 25 12 the the DET biotropia-163 25 13 antibiotic antibiotic ADJ biotropia-163 25 14 resistance resistance NOUN biotropia-163 25 15 of of ADP biotropia-163 25 16 e. e. PROPN biotropia-163 25 17 coli coli PROPN biotropia-163 25 18 in in ADP biotropia-163 25 19 the the DET biotropia-163 25 20 digestive digestive ADJ biotropia-163 25 21 system system NOUN biotropia-163 25 22 of of ADP biotropia-163 25 23 varanus varanus PROPN biotropia-163 25 24 spp spp NOUN biotropia-163 25 25 . . PUNCT biotropia-163 26 1 in in ADP biotropia-163 26 2 this this DET biotropia-163 26 3 experiment experiment NOUN biotropia-163 26 4 , , PUNCT biotropia-163 26 5 an an DET biotropia-163 26 6 antibiotic antibiotic ADJ biotropia-163 26 7 - - PUNCT biotropia-163 26 8 resistant resistant ADJ biotropia-163 26 9 gene gene NOUN biotropia-163 26 10 from from ADP biotropia-163 26 11 e. e. PROPN biotropia-163 26 12 coli coli PROPN biotropia-163 26 13 was be AUX biotropia-163 26 14 isolated isolate VERB biotropia-163 26 15 and and CCONJ biotropia-163 26 16 further far ADV biotropia-163 26 17 characterized characterize VERB biotropia-163 26 18 by by ADP biotropia-163 26 19 dna dna PROPN biotropia-163 26 20 sequence sequence NOUN biotropia-163 26 21 analysis analysis NOUN biotropia-163 26 22 . . PUNCT biotropia-163 27 1 11 11 NUM biotropia-163 27 2 a a DET biotropia-163 27 3 novel novel ADJ biotropia-163 27 4 integron integron NOUN biotropia-163 27 5 in in ADP biotropia-163 27 6 ther ther ADJ biotropia-163 27 7 genome genome NOUN biotropia-163 27 8 of of ADP biotropia-163 27 9 escherichia escherichia NOUN biotropia-163 27 10 coli coli NOUN biotropia-163 27 11 – – PUNCT biotropia-163 27 12 esti esti PROPN biotropia-163 27 13 puspitasari puspitasari PROPN biotropia-163 27 14 et et PROPN biotropia-163 27 15 al al PROPN biotropia-163 27 16 . . PROPN biotropia-163 27 17 material material NOUN biotropia-163 27 18 and and CCONJ biotropia-163 27 19 mothods mothod NOUN biotropia-163 27 20 bacterial bacterial ADJ biotropia-163 27 21 strains strain NOUN biotropia-163 27 22 and and CCONJ biotropia-163 27 23 plasmids plasmid NOUN biotropia-163 27 24 the the DET biotropia-163 27 25 bacterial bacterial ADJ biotropia-163 27 26 strains strain NOUN biotropia-163 27 27 and and CCONJ biotropia-163 27 28 p;asmids p;asmid NOUN biotropia-163 27 29 used use VERB biotropia-163 27 30 in in ADP biotropia-163 27 31 this this DET biotropia-163 27 32 research research NOUN biotropia-163 27 33 are be AUX biotropia-163 27 34 shown show VERB biotropia-163 27 35 in in ADP biotropia-163 27 36 table table NOUN biotropia-163 27 37 1 1 NUM biotropia-163 27 38 . . PUNCT biotropia-163 27 39 table table NOUN biotropia-163 27 40 1 1 NUM biotropia-163 27 41 . . PUNCT biotropia-163 28 1 bacterial bacterial ADJ biotropia-163 28 2 strains strain NOUN biotropia-163 28 3 plasmida plasmida ADJ biotropia-163 28 4 growth growth NOUN biotropia-163 28 5 conditions condition NOUN biotropia-163 28 6 antibiotic antibiotic ADJ biotropia-163 28 7 - - PUNCT biotropia-163 28 8 resistant resistant ADJ biotropia-163 28 9 bacteria bacteria NOUN biotropia-163 28 10 were be AUX biotropia-163 28 11 cultured cultured ADJ biotropia-163 28 12 aerobic aerobic ADJ biotropia-163 28 13 ( ( PUNCT biotropia-163 28 14 100 100 NUM biotropia-163 28 15 rpm rpm NOUN biotropia-163 28 16 ) ) PUNCT biotropia-163 28 17 at at ADP biotropia-163 28 18 37 37 NUM biotropia-163 28 19 ° ° ADP biotropia-163 28 20 c c NOUN biotropia-163 28 21 and and CCONJ biotropia-163 28 22 kept keep VERB biotropia-163 28 23 overnight overnight ADV biotropia-163 28 24 in in ADP biotropia-163 28 25 luria luria PROPN biotropia-163 28 26 bertani bertani NOUN biotropia-163 28 27 ( ( PUNCT biotropia-163 28 28 lb lb X biotropia-163 28 29 ) ) PUNCT biotropia-163 28 30 medium medium NOUN biotropia-163 28 31 supplemented supplement VERB biotropia-163 28 32 with with ADP biotropia-163 28 33 one one NUM biotropia-163 28 34 , , PUNCT biotropia-163 28 35 or or CCONJ biotropia-163 28 36 a a DET biotropia-163 28 37 combination combination NOUN biotropia-163 28 38 of of ADP biotropia-163 28 39 the the DET biotropia-163 28 40 following following ADJ biotropia-163 28 41 antibiotics antibiotic NOUN biotropia-163 28 42 : : PUNCT biotropia-163 28 43 ampicillin ampicillin NOUN biotropia-163 28 44 ( ( PUNCT biotropia-163 28 45 100 100 NUM biotropia-163 28 46 u.g u.g PROPN biotropia-163 28 47 / / SYM biotropia-163 28 48 ml ml NOUN biotropia-163 28 49 ) ) PUNCT biotropia-163 28 50 , , PUNCT biotropia-163 28 51 streptomycin streptomycin PROPN biotropia-163 28 52 ( ( PUNCT biotropia-163 28 53 50 50 NUM biotropia-163 28 54 ng ng NOUN biotropia-163 28 55 / / SYM biotropia-163 28 56 ml ml NOUN biotropia-163 28 57 ) ) PUNCT biotropia-163 28 58 , , PUNCT biotropia-163 28 59 spectinomycin spectinomycin PROPN biotropia-163 28 60 ( ( PUNCT biotropia-163 28 61 50 50 NUM biotropia-163 28 62 ng ng NOUN biotropia-163 28 63 / / SYM biotropia-163 28 64 ml ml NOUN biotropia-163 28 65 ) ) PUNCT biotropia-163 28 66 . . PUNCT biotropia-163 29 1 dna dna PROPN biotropia-163 29 2 extraction extraction NOUN biotropia-163 29 3 bacterial bacterial ADJ biotropia-163 29 4 isolates isolate NOUN biotropia-163 29 5 were be AUX biotropia-163 29 6 grown grow VERB biotropia-163 29 7 in in ADP biotropia-163 29 8 10 10 NUM biotropia-163 29 9 ml ml NOUN biotropia-163 29 10 lb lb NOUN biotropia-163 29 11 in in ADP biotropia-163 29 12 the the DET biotropia-163 29 13 presence presence NOUN biotropia-163 29 14 of of ADP biotropia-163 29 15 a a DET biotropia-163 29 16 selective selective ADJ biotropia-163 29 17 antibiotic antibiotic NOUN biotropia-163 29 18 at at ADP biotropia-163 29 19 37 37 NUM biotropia-163 29 20 ° ° NOUN biotropia-163 29 21 c c NOUN biotropia-163 29 22 overnight overnight ADV biotropia-163 29 23 . . PUNCT biotropia-163 30 1 alkaline alkaline NOUN biotropia-163 30 2 lysis lysis NOUN biotropia-163 30 3 method method NOUN biotropia-163 30 4 ( ( PUNCT biotropia-163 30 5 sambrook sambrook PROPN biotropia-163 30 6 et et PROPN biotropia-163 30 7 al al PROPN biotropia-163 30 8 . . PROPN biotropia-163 30 9 1989 1989 NUM biotropia-163 30 10 ) ) PUNCT biotropia-163 30 11 was be AUX biotropia-163 30 12 used use VERB biotropia-163 30 13 for for ADP biotropia-163 30 14 plasmid plasmid ADJ biotropia-163 30 15 dna dna PROPN biotropia-163 30 16 extraction extraction NOUN biotropia-163 30 17 , , PUNCT biotropia-163 30 18 and and CCONJ biotropia-163 30 19 wizard wizard ADJ biotropia-163 30 20 ® ® NOUN biotropia-163 30 21 genomic genomic ADJ biotropia-163 30 22 dna dna PROPN biotropia-163 30 23 purification purification NOUN biotropia-163 30 24 kit kit NOUN biotropia-163 30 25 ( ( PUNCT biotropia-163 30 26 promega promega PROPN biotropia-163 30 27 , , PUNCT biotropia-163 30 28 madison madison PROPN biotropia-163 30 29 , , PUNCT biotropia-163 30 30 wi wi PROPN biotropia-163 30 31 ) ) PUNCT biotropia-163 30 32 was be AUX biotropia-163 30 33 used use VERB biotropia-163 30 34 according accord VERB biotropia-163 30 35 to to ADP biotropia-163 30 36 manufacturer manufacturer NOUN biotropia-163 30 37 protocol protocol NOUN biotropia-163 30 38 for for ADP biotropia-163 30 39 genomic genomic ADJ biotropia-163 30 40 dna dna PROPN biotropia-163 30 41 extraction extraction NOUN biotropia-163 30 42 . . PUNCT biotropia-163 31 1 12 12 NUM biotropia-163 31 2 biotropia biotropia NOUN biotropia-163 31 3 no no NOUN biotropia-163 31 4 . . PROPN biotropia-163 31 5 16 16 NUM biotropia-163 31 6 , , PUNCT biotropia-163 31 7 2001 2001 NUM biotropia-163 31 8 pcr pcr ADJ biotropia-163 31 9 amplification amplification NOUN biotropia-163 31 10 pcr pcr NOUN biotropia-163 31 11 were be AUX biotropia-163 31 12 performed perform VERB biotropia-163 31 13 in in ADP biotropia-163 31 14 gene gene NOUN biotropia-163 31 15 amp amp PROPN biotropia-163 31 16 ® ® NOUN biotropia-163 31 17 pcr pcr ADJ biotropia-163 31 18 system system NOUN biotropia-163 31 19 2400 2400 NUM biotropia-163 31 20 ( ( PUNCT biotropia-163 31 21 perkin perkin PROPN biotropia-163 31 22 elmer elmer PROPN biotropia-163 31 23 , , PUNCT biotropia-163 31 24 branchburg branchburg PROPN biotropia-163 31 25 , , PUNCT biotropia-163 31 26 new new PROPN biotropia-163 31 27 jersey jersey PROPN biotropia-163 31 28 ) ) PUNCT biotropia-163 31 29 . . PUNCT biotropia-163 32 1 ready ready ADJ biotropia-163 32 2 to to PART biotropia-163 32 3 go go VERB biotropia-163 32 4 ™ ™ VERB biotropia-163 32 5 pcr pcr NOUN biotropia-163 32 6 beads bead NOUN biotropia-163 32 7 ( ( PUNCT biotropia-163 32 8 pharmacia pharmacia PROPN biotropia-163 32 9 biotech biotech NOUN biotropia-163 32 10 , , PUNCT biotropia-163 32 11 uppsala uppsala PROPN biotropia-163 32 12 , , PUNCT biotropia-163 32 13 sweden sweden PROPN biotropia-163 32 14 ) ) PUNCT biotropia-163 32 15 was be AUX biotropia-163 32 16 used use VERB biotropia-163 32 17 for for ADP biotropia-163 32 18 each each DET biotropia-163 32 19 pcr pcr ADJ biotropia-163 32 20 reaction reaction NOUN biotropia-163 32 21 . . PUNCT biotropia-163 33 1 each each PRON biotropia-163 33 2 of of ADP biotropia-163 33 3 25 25 NUM biotropia-163 33 4 ul ul NOUN biotropia-163 33 5 reaction reaction NOUN biotropia-163 33 6 mixtures mixture NOUN biotropia-163 33 7 contained contain VERB biotropia-163 33 8 pcr pcr ADJ biotropia-163 33 9 beads bead NOUN biotropia-163 33 10 , , PUNCT biotropia-163 33 11 4 4 NUM biotropia-163 33 12 ng ng PROPN biotropia-163 33 13 / / SYM biotropia-163 33 14 ul ul NOUN biotropia-163 33 15 of of ADP biotropia-163 33 16 genomic genomic PROPN biotropia-163 33 17 dna dna NOUN biotropia-163 33 18 , , PUNCT biotropia-163 33 19 10 10 NUM biotropia-163 33 20 ng ng PROPN biotropia-163 33 21 / / SYM biotropia-163 33 22 ul ul NOUN biotropia-163 33 23 of of ADP biotropia-163 33 24 primers primer NOUN biotropia-163 33 25 ( ( PUNCT biotropia-163 33 26 5'cs 5'cs NUM biotropia-163 33 27 : : PUNCT biotropia-163 33 28 ggcatccaagcagca ggcatccaagcagca PROPN biotropia-163 33 29 ag ag PROPN biotropia-163 33 30 , , PUNCT biotropia-163 33 31 3'cs 3'cs NUM biotropia-163 33 32 : : PUNCT biotropia-163 33 33 aagcagacttgacctga aagcagacttgacctga ADJ biotropia-163 33 34 , , PUNCT biotropia-163 33 35 qac qac NOUN biotropia-163 33 36 : : PUNCT biotropia-163 33 37 atcgcaatagttggcgaagt atcgcaatagttggcgaagt PROPN biotropia-163 33 38 , , PUNCT biotropia-163 33 39 sul sul ADJ biotropia-163 33 40 : : PUNCT biotropia-163 33 41 gcaaggcggaaacccgcgcc gcaaggcggaaacccgcgcc NOUN biotropia-163 33 42 ) ) PUNCT biotropia-163 33 43 and and CCONJ biotropia-163 33 44 distilled distil VERB biotropia-163 33 45 water water NOUN biotropia-163 33 46 up up ADP biotropia-163 33 47 to to PART biotropia-163 33 48 25 25 NUM biotropia-163 33 49 ul ul NOUN biotropia-163 33 50 . . PUNCT biotropia-163 33 51 pcr pcr ADJ biotropia-163 33 52 condition condition NOUN biotropia-163 33 53 for for ADP biotropia-163 33 54 the the DET biotropia-163 33 55 integron integron NOUN biotropia-163 33 56 was be AUX biotropia-163 33 57 amplified amplify VERB biotropia-163 33 58 in in ADP biotropia-163 33 59 35 35 NUM biotropia-163 33 60 thermal thermal ADJ biotropia-163 33 61 cycles cycle NOUN biotropia-163 33 62 at at ADP biotropia-163 33 63 94 94 NUM biotropia-163 33 64 ° ° ADP biotropia-163 33 65 c c NOUN biotropia-163 33 66 for for ADP biotropia-163 33 67 1 1 NUM biotropia-163 33 68 minute minute NOUN biotropia-163 33 69 , , PUNCT biotropia-163 33 70 55 55 NUM biotropia-163 34 1 ° ° NOUN biotropia-163 34 2 c c NOUN biotropia-163 34 3 for for ADP biotropia-163 34 4 1 1 NUM biotropia-163 34 5 minute minute NOUN biotropia-163 34 6 , , PUNCT biotropia-163 34 7 and and CCONJ biotropia-163 34 8 72 72 NUM biotropia-163 34 9 ° ° NOUN biotropia-163 34 10 c c NOUN biotropia-163 34 11 for for ADP biotropia-163 34 12 3 3 NUM biotropia-163 34 13 minutes minute NOUN biotropia-163 34 14 . . PUNCT biotropia-163 35 1 a a DET biotropia-163 35 2 final final ADJ biotropia-163 35 3 extension extension NOUN biotropia-163 35 4 step step NOUN biotropia-163 35 5 for for ADP biotropia-163 35 6 7 7 NUM biotropia-163 35 7 minutes minute NOUN biotropia-163 35 8 at at ADP biotropia-163 35 9 72 72 NUM biotropia-163 35 10 ° ° NOUN biotropia-163 35 11 c c NOUN biotropia-163 35 12 was be AUX biotropia-163 35 13 also also ADV biotropia-163 35 14 included include VERB biotropia-163 35 15 . . PUNCT biotropia-163 36 1 the the DET biotropia-163 36 2 primers primer NOUN biotropia-163 36 3 used use VERB biotropia-163 36 4 were be AUX biotropia-163 36 5 5'cs 5'cs NUM biotropia-163 36 6 and and CCONJ biotropia-163 36 7 3'cs 3'c NOUN biotropia-163 36 8 that that PRON biotropia-163 36 9 amplify amplify VERB biotropia-163 36 10 variable variable ADJ biotropia-163 36 11 regions region NOUN biotropia-163 36 12 of of ADP biotropia-163 36 13 integron integron PROPN biotropia-163 36 14 , , PUNCT biotropia-163 36 15 qac qac PROPN biotropia-163 36 16 and and CCONJ biotropia-163 36 17 sitll sitll NOUN biotropia-163 36 18 primers primer NOUN biotropia-163 36 19 to to PART biotropia-163 36 20 amplify amplify VERB biotropia-163 36 21 the the DET biotropia-163 36 22 conserved conserved ADJ biotropia-163 36 23 region region NOUN biotropia-163 36 24 . . PUNCT biotropia-163 37 1 pcr pcr ADJ biotropia-163 37 2 product product NOUN biotropia-163 37 3 was be AUX biotropia-163 37 4 purified purify VERB biotropia-163 37 5 using use VERB biotropia-163 37 6 gene gene NOUN biotropia-163 37 7 clean clean ADJ biotropia-163 37 8 kit kit NOUN biotropia-163 37 9 ( ( PUNCT biotropia-163 37 10 bio bio NOUN biotropia-163 37 11 101 101 NUM biotropia-163 37 12 , , PUNCT biotropia-163 37 13 la la PROPN biotropia-163 37 14 jolla jolla PROPN biotropia-163 37 15 , , PUNCT biotropia-163 37 16 california california PROPN biotropia-163 37 17 ) ) PUNCT biotropia-163 37 18 . . PUNCT biotropia-163 38 1 cloning cloning NOUN biotropia-163 38 2 and and CCONJ biotropia-163 38 3 transformation transformation NOUN biotropia-163 38 4 amplified amplify VERB biotropia-163 38 5 dna dna PROPN biotropia-163 38 6 was be AUX biotropia-163 38 7 ligated ligate VERB biotropia-163 38 8 into into ADP biotropia-163 38 9 pas900 pas900 PROPN biotropia-163 38 10 ( ( PUNCT biotropia-163 38 11 kmr kmr PROPN biotropia-163 38 12 ) ) PUNCT biotropia-163 38 13 and and CCONJ biotropia-163 38 14 pgem pgem NOUN biotropia-163 38 15 - - PUNCT biotropia-163 38 16 t t NOUN biotropia-163 38 17 easy easy ADJ biotropia-163 38 18 ( ( PUNCT biotropia-163 38 19 apr apr NOUN biotropia-163 38 20 ) ) PUNCT biotropia-163 38 21 vector vector NOUN biotropia-163 38 22 . . PUNCT biotropia-163 39 1 the the DET biotropia-163 39 2 approximate approximate ADJ biotropia-163 39 3 ratio ratio NOUN biotropia-163 39 4 of of ADP biotropia-163 39 5 pas900 pas900 ADJ biotropia-163 39 6 vector vector NOUN biotropia-163 39 7 to to PART biotropia-163 39 8 insert insert VERB biotropia-163 39 9 dna dna PROPN biotropia-163 39 10 were be AUX biotropia-163 39 11 1:4 1:4 NUM biotropia-163 39 12 and and CCONJ biotropia-163 39 13 that that PRON biotropia-163 39 14 of of ADP biotropia-163 39 15 pgem pgem PROPN biotropia-163 39 16 - - PUNCT biotropia-163 39 17 t t NOUN biotropia-163 39 18 easy easy ADJ biotropia-163 39 19 to to PART biotropia-163 39 20 insert insert VERB biotropia-163 39 21 dna dna PROPN biotropia-163 39 22 was be AUX biotropia-163 39 23 1 1 NUM biotropia-163 39 24 to to ADP biotropia-163 39 25 16 16 NUM biotropia-163 39 26 . . PUNCT biotropia-163 40 1 ligation ligation NOUN biotropia-163 40 2 reaction reaction NOUN biotropia-163 40 3 consisted consist VERB biotropia-163 40 4 of of ADP biotropia-163 40 5 vectorinsert vectorinsert NOUN biotropia-163 40 6 mixture mixture NOUN biotropia-163 40 7 1 1 NUM biotropia-163 40 8 ul ul NOUN biotropia-163 40 9 of of ADP biotropia-163 40 10 t4ligase t4ligase PROPN biotropia-163 40 11 , , PUNCT biotropia-163 40 12 ix ix ADP biotropia-163 40 13 ligation ligation NOUN biotropia-163 40 14 buffer buffer NOUN biotropia-163 40 15 , , PUNCT biotropia-163 40 16 and and CCONJ biotropia-163 40 17 distilled distil VERB biotropia-163 40 18 water water NOUN biotropia-163 40 19 up up ADP biotropia-163 40 20 to to PART biotropia-163 40 21 20 20 NUM biotropia-163 40 22 ul ul INTJ biotropia-163 40 23 . . PUNCT biotropia-163 41 1 ligation ligation NOUN biotropia-163 41 2 mixture mixture NOUN biotropia-163 41 3 was be AUX biotropia-163 41 4 incubated incubate VERB biotropia-163 41 5 at at ADP biotropia-163 41 6 7 7 NUM biotropia-163 41 7 ° ° ADP biotropia-163 41 8 c c NOUN biotropia-163 41 9 for for ADP biotropia-163 41 10 16 16 NUM biotropia-163 41 11 - - SYM biotropia-163 41 12 18 18 NUM biotropia-163 41 13 h. h. NOUN biotropia-163 41 14 the the DET biotropia-163 41 15 ligation ligation NOUN biotropia-163 41 16 mixture mixture NOUN biotropia-163 41 17 was be AUX biotropia-163 41 18 transformed transform VERB biotropia-163 41 19 into into ADP biotropia-163 41 20 250 250 NUM biotropia-163 41 21 ul ul NOUN biotropia-163 41 22 of of ADP biotropia-163 41 23 chemically chemically ADV biotropia-163 41 24 competent competent ADJ biotropia-163 41 25 e. e. PROPN biotropia-163 41 26 coli coli PROPN biotropia-163 41 27 top10 top10 PROPN biotropia-163 41 28 , , PUNCT biotropia-163 41 29 followed follow VERB biotropia-163 41 30 by by ADP biotropia-163 41 31 heat heat NOUN biotropia-163 41 32 shocked shock VERB biotropia-163 41 33 at at ADP biotropia-163 41 34 42 42 NUM biotropia-163 41 35 ° ° ADP biotropia-163 41 36 c c NOUN biotropia-163 41 37 for for ADP biotropia-163 41 38 45 45 NUM biotropia-163 41 39 seconds second NOUN biotropia-163 41 40 . . PUNCT biotropia-163 42 1 after after ADP biotropia-163 42 2 the the DET biotropia-163 42 3 addition addition NOUN biotropia-163 42 4 of of ADP biotropia-163 42 5 3 3 NUM biotropia-163 42 6 ml ml NOUN biotropia-163 42 7 lb lb NOUN biotropia-163 42 8 , , PUNCT biotropia-163 42 9 the the DET biotropia-163 42 10 culture culture NOUN biotropia-163 42 11 was be AUX biotropia-163 42 12 incubated incubate VERB biotropia-163 42 13 with with ADP biotropia-163 42 14 vigorous vigorous ADJ biotropia-163 42 15 shaking shaking NOUN biotropia-163 42 16 at at ADP biotropia-163 42 17 37 37 NUM biotropia-163 42 18 ° ° ADP biotropia-163 42 19 c c NOUN biotropia-163 42 20 for for ADP biotropia-163 42 21 90 90 NUM biotropia-163 42 22 minutes minute NOUN biotropia-163 42 23 ( ( PUNCT biotropia-163 42 24 sambrook sambrook PROPN biotropia-163 42 25 et et PROPN biotropia-163 42 26 al al PROPN biotropia-163 42 27 . . PROPN biotropia-163 42 28 1989 1989 NUM biotropia-163 42 29 ) ) PUNCT biotropia-163 42 30 . . PUNCT biotropia-163 43 1 transformants transformant NOUN biotropia-163 43 2 were be AUX biotropia-163 43 3 then then ADV biotropia-163 43 4 selected select VERB biotropia-163 43 5 on on ADP biotropia-163 43 6 luria luria PROPN biotropia-163 43 7 bertani bertani PROPN biotropia-163 43 8 agar agar NOUN biotropia-163 43 9 ( ( PUNCT biotropia-163 43 10 lba lba PROPN biotropia-163 43 11 ) ) PUNCT biotropia-163 43 12 supplemented supplement VERB biotropia-163 43 13 with with ADP biotropia-163 43 14 x x NOUN biotropia-163 43 15 - - NOUN biotropia-163 43 16 gal gal ADJ biotropia-163 43 17 ( ( PUNCT biotropia-163 43 18 40ug 40ug NOUN biotropia-163 43 19 / / SYM biotropia-163 43 20 ml ml NOUN biotropia-163 43 21 ) ) PUNCT biotropia-163 43 22 and and CCONJ biotropia-163 43 23 appropriate appropriate ADJ biotropia-163 43 24 antibiotic antibiotic ADJ biotropia-163 43 25 / / SYM biotropia-163 43 26 s. s. PROPN biotropia-163 43 27 dna dna PROPN biotropia-163 43 28 sequencing sequence VERB biotropia-163 43 29 ps2 ps2 PROPN biotropia-163 43 30 t t PROPN biotropia-163 43 31 double double ADJ biotropia-163 43 32 stranded strand VERB biotropia-163 43 33 templates template NOUN biotropia-163 43 34 were be AUX biotropia-163 43 35 derived derive VERB biotropia-163 43 36 from from ADP biotropia-163 43 37 cloning cloning NOUN biotropia-163 43 38 of of ADP biotropia-163 43 39 sti sti PROPN biotropia-163 43 40 in in ADP biotropia-163 43 41 pgem pgem PROPN biotropia-163 43 42 - - PUNCT biotropia-163 43 43 t t NOUN biotropia-163 43 44 easy easy ADJ biotropia-163 43 45 . . PUNCT biotropia-163 44 1 sequencing sequence VERB biotropia-163 44 2 reaction reaction NOUN biotropia-163 44 3 consisted consist VERB biotropia-163 44 4 of of ADP biotropia-163 44 5 8 8 NUM biotropia-163 44 6 ul ul INTJ biotropia-163 44 7 big big ADJ biotropia-163 44 8 dye dye NOUN biotropia-163 44 9 - - PUNCT biotropia-163 44 10 terminator terminator NOUN biotropia-163 44 11 , , PUNCT biotropia-163 44 12 360 360 NUM biotropia-163 44 13 ng ng PROPN biotropia-163 44 14 of of ADP biotropia-163 44 15 dna dna PROPN biotropia-163 44 16 template template NOUN biotropia-163 44 17 , , PUNCT biotropia-163 44 18 8 8 NUM biotropia-163 44 19 ng ng PROPN biotropia-163 44 20 / / SYM biotropia-163 44 21 ul ul PROPN biotropia-163 44 22 of of ADP biotropia-163 44 23 primer primer NOUN biotropia-163 44 24 and and CCONJ biotropia-163 44 25 distilled distil VERB biotropia-163 44 26 water water NOUN biotropia-163 44 27 up up ADP biotropia-163 44 28 to to PART biotropia-163 44 29 20 20 NUM biotropia-163 44 30 ul ul NOUN biotropia-163 44 31 cycle cycle NOUN biotropia-163 44 32 sequencing sequencing NOUN biotropia-163 44 33 was be AUX biotropia-163 44 34 performed perform VERB biotropia-163 44 35 using use VERB biotropia-163 44 36 pcr pcr ADJ biotropia-163 44 37 machine machine NOUN biotropia-163 44 38 with with ADP biotropia-163 44 39 three three NUM biotropia-163 44 40 - - PUNCT biotropia-163 44 41 step step NOUN biotropia-163 44 42 profile profile NOUN biotropia-163 44 43 for for ADP biotropia-163 44 44 25 25 NUM biotropia-163 44 45 cycles cycle NOUN biotropia-163 44 46 : : PUNCT biotropia-163 44 47 a a DET biotropia-163 44 48 10 10 NUM biotropia-163 44 49 - - PUNCT biotropia-163 44 50 second second NOUN biotropia-163 44 51 denaturation denaturation NOUN biotropia-163 44 52 at at ADP biotropia-163 44 53 96 96 NUM biotropia-163 44 54 ° ° NOUN biotropia-163 44 55 c c NOUN biotropia-163 44 56 , , PUNCT biotropia-163 44 57 a a DET biotropia-163 44 58 5 5 NUM biotropia-163 44 59 - - PUNCT biotropia-163 44 60 second second NOUN biotropia-163 44 61 annealing annealing NOUN biotropia-163 44 62 at at ADP biotropia-163 44 63 50 50 NUM biotropia-163 44 64 ° ° NOUN biotropia-163 44 65 c c NOUN biotropia-163 44 66 , , PUNCT biotropia-163 44 67 and and CCONJ biotropia-163 44 68 a a DET biotropia-163 44 69 4 4 NUM biotropia-163 44 70 - - PUNCT biotropia-163 44 71 minute minute NOUN biotropia-163 44 72 extension extension NOUN biotropia-163 44 73 at at ADP biotropia-163 44 74 60 60 NUM biotropia-163 44 75 ° ° NOUN biotropia-163 44 76 c c NOUN biotropia-163 44 77 . . PUNCT biotropia-163 45 1 cycle cycle NOUN biotropia-163 45 2 sequencing sequence VERB biotropia-163 45 3 product product NOUN biotropia-163 45 4 was be AUX biotropia-163 45 5 purified purify VERB biotropia-163 45 6 by by ADP biotropia-163 45 7 ethanol ethanol NOUN biotropia-163 45 8 - - PUNCT biotropia-163 45 9 sodium sodium NOUN biotropia-163 45 10 acetate acetate NOUN biotropia-163 45 11 precipitation precipitation NOUN biotropia-163 45 12 method method NOUN biotropia-163 45 13 . . PUNCT biotropia-163 46 1 a a DET biotropia-163 46 2 1.5 1.5 NUM biotropia-163 46 3 ml ml NOUN biotropia-163 46 4 microcentrifuge microcentrifuge ADJ biotropia-163 46 5 tube tube NOUN biotropia-163 46 6 was be AUX biotropia-163 46 7 filled fill VERB biotropia-163 46 8 with with ADP biotropia-163 46 9 2 2 NUM biotropia-163 46 10 ul ul NOUN biotropia-163 46 11 of of ADP biotropia-163 46 12 sodium sodium NOUN biotropia-163 46 13 acetate acetate NOUN biotropia-163 46 14 ( ( PUNCT biotropia-163 46 15 ph ph NOUN biotropia-163 46 16 4.6 4.6 NUM biotropia-163 46 17 ) ) PUNCT biotropia-163 46 18 , , PUNCT biotropia-163 46 19 50 50 NUM biotropia-163 46 20 ul ul NOUN biotropia-163 46 21 of of ADP biotropia-163 46 22 ethanol ethanol NOUN biotropia-163 46 23 95 95 NUM biotropia-163 46 24 % % NOUN biotropia-163 46 25 , , PUNCT biotropia-163 46 26 and and CCONJ biotropia-163 46 27 20 20 NUM biotropia-163 46 28 ul ul NOUN biotropia-163 46 29 of of ADP biotropia-163 46 30 the the DET biotropia-163 46 31 cycle cycle NOUN biotropia-163 46 32 sequencing sequence VERB biotropia-163 46 33 mixture mixture NOUN biotropia-163 46 34 , , PUNCT biotropia-163 46 35 and and CCONJ biotropia-163 46 36 then then ADV biotropia-163 46 37 incubated incubate VERB biotropia-163 46 38 at at ADP biotropia-163 46 39 room room NOUN biotropia-163 46 40 temperature temperature NOUN biotropia-163 46 41 for for ADP biotropia-163 46 42 30 30 NUM biotropia-163 46 43 minutes minute NOUN biotropia-163 46 44 . . PUNCT biotropia-163 47 1 the the DET biotropia-163 47 2 mixture mixture NOUN biotropia-163 47 3 was be AUX biotropia-163 47 4 centrifuged centrifuge VERB biotropia-163 47 5 at at ADP biotropia-163 47 6 maximum maximum ADJ biotropia-163 47 7 speed speed NOUN biotropia-163 47 8 for for ADP biotropia-163 47 9 20 20 NUM biotropia-163 47 10 minutes minute NOUN biotropia-163 47 11 . . PUNCT biotropia-163 48 1 the the DET biotropia-163 48 2 supernatant supernatant NOUN biotropia-163 48 3 was be AUX biotropia-163 48 4 removed remove VERB biotropia-163 48 5 from from ADP biotropia-163 48 6 the the DET biotropia-163 48 7 tube tube NOUN biotropia-163 48 8 , , PUNCT biotropia-163 48 9 and and CCONJ biotropia-163 48 10 250 250 NUM biotropia-163 48 11 ul ul NOUN biotropia-163 48 12 of of ADP biotropia-163 48 13 70 70 NUM biotropia-163 48 14 % % NOUN biotropia-163 48 15 ethanol ethanol NOUN biotropia-163 48 16 was be AUX biotropia-163 48 17 added add VERB biotropia-163 48 18 into into ADP biotropia-163 48 19 the the DET biotropia-163 48 20 tube tube NOUN biotropia-163 48 21 . . PUNCT biotropia-163 49 1 the the DET biotropia-163 49 2 mixture mixture NOUN biotropia-163 49 3 was be AUX biotropia-163 49 4 centrifuged centrifuge VERB biotropia-163 49 5 again again ADV biotropia-163 49 6 for for ADP biotropia-163 49 7 5 5 NUM biotropia-163 49 8 minutes minute NOUN biotropia-163 49 9 . . PUNCT biotropia-163 50 1 the the DET biotropia-163 50 2 supernatant supernatant NOUN biotropia-163 50 3 was be AUX biotropia-163 50 4 removed remove VERB biotropia-163 50 5 and and CCONJ biotropia-163 50 6 the the DET biotropia-163 50 7 dna dna PROPN biotropia-163 50 8 pellet pellet NOUN biotropia-163 50 9 was be AUX biotropia-163 50 10 vacuum vacuum NOUN biotropia-163 50 11 - - PUNCT biotropia-163 50 12 dried dry VERB biotropia-163 50 13 at at ADP biotropia-163 50 14 50 50 NUM biotropia-163 50 15 cm cm NUM biotropia-163 50 16 hg hg NOUN biotropia-163 50 17 for for ADP biotropia-163 50 18 10 10 NUM biotropia-163 50 19 minutes minute NOUN biotropia-163 50 20 . . PUNCT biotropia-163 51 1 13 13 NUM biotropia-163 51 2 a a DET biotropia-163 51 3 novel novel ADJ biotropia-163 51 4 integron integron NOUN biotropia-163 51 5 in in ADP biotropia-163 51 6 the the DET biotropia-163 51 7 genome genome NOUN biotropia-163 51 8 of of ADP biotropia-163 51 9 escherichia escherichia NOUN biotropia-163 51 10 coli coli NOUN biotropia-163 51 11 esti esti PROPN biotropia-163 51 12 puspitasari puspitasari PROPN biotropia-163 51 13 et et PROPN biotropia-163 51 14 al al PROPN biotropia-163 51 15 . . PUNCT biotropia-163 52 1 the the DET biotropia-163 52 2 dna dna PROPN biotropia-163 52 3 was be AUX biotropia-163 52 4 electrophoresed electrophorese VERB biotropia-163 52 5 according accord VERB biotropia-163 52 6 to to ADP biotropia-163 52 7 sambrook sambrook PROPN biotropia-163 52 8 et et PROPN biotropia-163 52 9 al al PROPN biotropia-163 52 10 . . PROPN biotropia-163 53 1 ( ( PUNCT biotropia-163 53 2 1989 1989 NUM biotropia-163 53 3 ) ) PUNCT biotropia-163 53 4 . . PUNCT biotropia-163 54 1 prior prior ADV biotropia-163 54 2 to to ADP biotropia-163 54 3 electrophoresis electrophoresis NOUN biotropia-163 54 4 , , PUNCT biotropia-163 54 5 dna dna PROPN biotropia-163 54 6 was be AUX biotropia-163 54 7 denatured denature VERB biotropia-163 54 8 with with ADP biotropia-163 54 9 6 6 NUM biotropia-163 54 10 ul ul NOUN biotropia-163 54 11 of of ADP biotropia-163 54 12 loading loading NOUN biotropia-163 54 13 buffer buffer NOUN biotropia-163 54 14 containing contain VERB biotropia-163 54 15 blue blue ADJ biotropia-163 54 16 dextran dextran NOUN biotropia-163 54 17 and and CCONJ biotropia-163 54 18 25 25 NUM biotropia-163 54 19 mm mm NOUN biotropia-163 54 20 of of ADP biotropia-163 54 21 edta edta NOUN biotropia-163 54 22 in in ADP biotropia-163 54 23 formamide formamide ADV biotropia-163 54 24 at at ADP biotropia-163 54 25 95 95 NUM biotropia-163 54 26 ° ° ADP biotropia-163 54 27 c c NOUN biotropia-163 54 28 for for ADP biotropia-163 54 29 2 2 NUM biotropia-163 54 30 minutes minute NOUN biotropia-163 54 31 , , PUNCT biotropia-163 54 32 and and CCONJ biotropia-163 54 33 quickly quickly ADV biotropia-163 54 34 placed place VERB biotropia-163 54 35 on on ADP biotropia-163 54 36 ice ice NOUN biotropia-163 54 37 . . PUNCT biotropia-163 55 1 approximately approximately ADV biotropia-163 55 2 1.5 1.5 NUM biotropia-163 55 3 ul ul NOUN biotropia-163 55 4 of of ADP biotropia-163 55 5 dna dna PROPN biotropia-163 55 6 sample sample NOUN biotropia-163 55 7 was be AUX biotropia-163 55 8 loaded load VERB biotropia-163 55 9 to to ADP biotropia-163 55 10 each each DET biotropia-163 55 11 well well ADV biotropia-163 55 12 and and CCONJ biotropia-163 55 13 ran run VERB biotropia-163 55 14 for for ADP biotropia-163 55 15 10 10 NUM biotropia-163 55 16 hours hour NOUN biotropia-163 55 17 . . PUNCT biotropia-163 56 1 the the DET biotropia-163 56 2 dna dna PROPN biotropia-163 56 3 was be AUX biotropia-163 56 4 compared compare VERB biotropia-163 56 5 with with ADP biotropia-163 56 6 those those PRON biotropia-163 56 7 in in ADP biotropia-163 56 8 the the DET biotropia-163 56 9 genbank genbank PROPN biotropia-163 56 10 and and CCONJ biotropia-163 56 11 embl embl PROPN biotropia-163 56 12 databases database NOUN biotropia-163 56 13 . . PUNCT biotropia-163 57 1 results result NOUN biotropia-163 57 2 and and CCONJ biotropia-163 57 3 discussions discussion NOUN biotropia-163 57 4 integron integron NOUN biotropia-163 57 5 detection detection NOUN biotropia-163 57 6 by by ADP biotropia-163 57 7 pcr pcr ADJ biotropia-163 57 8 amplification amplification NOUN biotropia-163 57 9 of of ADP biotropia-163 57 10 dna dna PROPN biotropia-163 57 11 sequence sequence NOUN biotropia-163 57 12 experiments experiment NOUN biotropia-163 57 13 using use VERB biotropia-163 57 14 qac qac NOUN biotropia-163 57 15 - - PUNCT biotropia-163 57 16 sul sul NOUN biotropia-163 57 17 primers primer NOUN biotropia-163 57 18 indicated indicate VERB biotropia-163 57 19 that that SCONJ biotropia-163 57 20 there there PRON biotropia-163 57 21 were be VERB biotropia-163 57 22 eight eight NUM biotropia-163 57 23 out out ADP biotropia-163 57 24 of of ADP biotropia-163 57 25 nine nine NUM biotropia-163 57 26 e.coli e.coli NOUN biotropia-163 57 27 isolates isolate NOUN biotropia-163 57 28 possessing possess VERB biotropia-163 57 29 3'conserved 3'conserved NUM biotropia-163 57 30 segment segment NOUN biotropia-163 57 31 of of ADP biotropia-163 57 32 the the DET biotropia-163 57 33 integron integron NOUN biotropia-163 57 34 ( ( PUNCT biotropia-163 57 35 table table NOUN biotropia-163 57 36 2 2 NUM biotropia-163 57 37 ) ) PUNCT biotropia-163 57 38 . . PUNCT biotropia-163 58 1 e. e. PROPN biotropia-163 58 2 coli coli PROPN biotropia-163 58 3 top top NOUN biotropia-163 58 4 10 10 NUM biotropia-163 58 5 containing contain VERB biotropia-163 58 6 recombinant recombinant ADJ biotropia-163 58 7 plasmid plasmid ADJ biotropia-163 58 8 pepio pepio NOUN biotropia-163 58 9 did do AUX biotropia-163 58 10 not not PART biotropia-163 58 11 show show VERB biotropia-163 58 12 integron integron NOUN biotropia-163 58 13 amplification amplification NOUN biotropia-163 58 14 , , PUNCT biotropia-163 58 15 although although SCONJ biotropia-163 58 16 it it PRON biotropia-163 58 17 carries carry VERB biotropia-163 58 18 a a DET biotropia-163 58 19 10 10 NUM biotropia-163 58 20 - - PUNCT biotropia-163 58 21 kb kb PROPN biotropia-163 58 22 dna dna PROPN biotropia-163 58 23 fragment fragment PROPN biotropia-163 58 24 originated originate VERB biotropia-163 58 25 from from ADP biotropia-163 58 26 genomic genomic ADJ biotropia-163 58 27 dna dna NOUN biotropia-163 58 28 of of ADP biotropia-163 58 29 e.coli e.coli NOUN biotropia-163 58 30 sg2 sg2 PROPN biotropia-163 58 31 isolated isolate VERB biotropia-163 58 32 from from ADP biotropia-163 58 33 varanus varanus PROPN biotropia-163 58 34 spp spp PROPN biotropia-163 58 35 . . PUNCT biotropia-163 59 1 ( ( PUNCT biotropia-163 59 2 table table NOUN biotropia-163 59 3 1 1 NUM biotropia-163 59 4 ) ) PUNCT biotropia-163 59 5 . . PUNCT biotropia-163 60 1 we we PRON biotropia-163 60 2 suspected suspect VERB biotropia-163 60 3 that that SCONJ biotropia-163 60 4 parts part NOUN biotropia-163 60 5 of of ADP biotropia-163 60 6 the the DET biotropia-163 60 7 integron integron NOUN biotropia-163 60 8 have have AUX biotropia-163 60 9 been be AUX biotropia-163 60 10 deleted delete VERB biotropia-163 60 11 when when SCONJ biotropia-163 60 12 it it PRON biotropia-163 60 13 was be AUX biotropia-163 60 14 digested digest VERB biotropia-163 60 15 by by ADP biotropia-163 60 16 restriction restriction NOUN biotropia-163 60 17 enzymes enzyme NOUN biotropia-163 60 18 . . PUNCT biotropia-163 61 1 pcr pcr ADJ biotropia-163 61 2 product product NOUN biotropia-163 61 3 using use VERB biotropia-163 61 4 5'cs-3'cs 5'cs-3'cs NUM biotropia-163 61 5 primers primer NOUN biotropia-163 61 6 generated generate VERB biotropia-163 61 7 a a DET biotropia-163 61 8 single single ADJ biotropia-163 61 9 amplified amplify VERB biotropia-163 61 10 dna dna NOUN biotropia-163 61 11 band band NOUN biotropia-163 61 12 of of ADP biotropia-163 61 13 about about ADV biotropia-163 61 14 1 1 NUM biotropia-163 61 15 - - PUNCT biotropia-163 61 16 kb kb NOUN biotropia-163 61 17 . . PUNCT biotropia-163 62 1 it it PRON biotropia-163 62 2 was be AUX biotropia-163 62 3 generated generate VERB biotropia-163 62 4 from from ADP biotropia-163 62 5 genomic genomic ADJ biotropia-163 62 6 dna dna PROPN biotropia-163 62 7 of of ADP biotropia-163 62 8 e. e. PROPN biotropia-163 62 9 coli coli PROPN biotropia-163 62 10 sg2 sg2 PROPN biotropia-163 62 11 isolate isolate PROPN biotropia-163 62 12 , , PUNCT biotropia-163 62 13 which which PRON biotropia-163 62 14 was be AUX biotropia-163 62 15 designated designate VERB biotropia-163 62 16 as as ADP biotropia-163 62 17 sti sti PROPN biotropia-163 62 18 ( ( PUNCT biotropia-163 62 19 table table NOUN biotropia-163 62 20 2 2 NUM biotropia-163 62 21 and and CCONJ biotropia-163 62 22 fig.2 fig.2 PROPN biotropia-163 62 23 lane lane NOUN biotropia-163 62 24 3 3 NUM biotropia-163 62 25 ) ) PUNCT biotropia-163 62 26 . . PUNCT biotropia-163 63 1 at at ADP biotropia-163 63 2 the the DET biotropia-163 63 3 downstream downstream ADJ biotropia-163 63 4 end end NOUN biotropia-163 63 5 of of ADP biotropia-163 63 6 each each DET biotropia-163 63 7 resistant resistant ADJ biotropia-163 63 8 gene gene NOUN biotropia-163 63 9 cassette cassette NOUN biotropia-163 63 10 inserted insert VERB biotropia-163 63 11 in in ADP biotropia-163 63 12 the the DET biotropia-163 63 13 variable variable ADJ biotropia-163 63 14 region region NOUN biotropia-163 63 15 of of ADP biotropia-163 63 16 integrons integron NOUN biotropia-163 63 17 , , PUNCT biotropia-163 63 18 there there PRON biotropia-163 63 19 is be VERB biotropia-163 63 20 a a DET biotropia-163 63 21 short short ADJ biotropia-163 63 22 imperfect imperfect ADJ biotropia-163 63 23 inverted invert VERB biotropia-163 63 24 repeat repeat NOUN biotropia-163 63 25 element element NOUN biotropia-163 63 26 called call VERB biotropia-163 63 27 the the DET biotropia-163 63 28 59base 59base NUM biotropia-163 63 29 element element NOUN biotropia-163 63 30 . . PUNCT biotropia-163 64 1 each each DET biotropia-163 64 2 inserted insert VERB biotropia-163 64 3 gene gene NOUN biotropia-163 64 4 has have VERB biotropia-163 64 5 its its PRON biotropia-163 64 6 own own ADJ biotropia-163 64 7 version version NOUN biotropia-163 64 8 of of ADP biotropia-163 64 9 this this DET biotropia-163 64 10 element element NOUN biotropia-163 64 11 ( ( PUNCT biotropia-163 64 12 levesque levesque PROPN biotropia-163 64 13 et et PROPN biotropia-163 64 14 al al PROPN biotropia-163 64 15 . . PROPN biotropia-163 64 16 1995 1995 NUM biotropia-163 64 17 ) ) PUNCT biotropia-163 64 18 . . PUNCT biotropia-163 65 1 the the DET biotropia-163 65 2 sti sti PROPN biotropia-163 65 3 was be AUX biotropia-163 65 4 also also ADV biotropia-163 65 5 thought think VERB biotropia-163 65 6 to to PART biotropia-163 65 7 have have VERB biotropia-163 65 8 a a DET biotropia-163 65 9 59 59 NUM biotropia-163 65 10 - - PUNCT biotropia-163 65 11 base base NOUN biotropia-163 65 12 element element NOUN biotropia-163 65 13 , , PUNCT biotropia-163 65 14 although although SCONJ biotropia-163 65 15 it it PRON biotropia-163 65 16 was be AUX biotropia-163 65 17 not not PART biotropia-163 65 18 demonstrated demonstrate VERB biotropia-163 65 19 in in ADP biotropia-163 65 20 this this DET biotropia-163 65 21 study study NOUN biotropia-163 65 22 . . PUNCT biotropia-163 66 1 these these DET biotropia-163 66 2 59 59 NUM biotropia-163 66 3 - - PUNCT biotropia-163 66 4 base base NOUN biotropia-163 66 5 elements element NOUN biotropia-163 66 6 are be AUX biotropia-163 66 7 known know VERB biotropia-163 66 8 to to PART biotropia-163 66 9 be be AUX biotropia-163 66 10 important important ADJ biotropia-163 66 11 in in ADP biotropia-163 66 12 the the DET biotropia-163 66 13 recombination recombination NOUN biotropia-163 66 14 events event NOUN biotropia-163 66 15 observed observe VERB biotropia-163 66 16 in in ADP biotropia-163 66 17 the the DET biotropia-163 66 18 evolution evolution NOUN biotropia-163 66 19 of of ADP biotropia-163 66 20 the the DET biotropia-163 66 21 integron integron NOUN biotropia-163 66 22 . . PUNCT biotropia-163 67 1 for for ADP biotropia-163 67 2 example example NOUN biotropia-163 67 3 , , PUNCT biotropia-163 67 4 in in ADP biotropia-163 67 5 plasmid plasmid ADJ biotropia-163 67 6 pvsi pvsi NOUN biotropia-163 67 7 , , PUNCT biotropia-163 67 8 which which PRON biotropia-163 67 9 possesses possess VERB biotropia-163 67 10 the the DET biotropia-163 67 11 5 5 NUM biotropia-163 67 12 ' ' PUNCT biotropia-163 67 13 and and CCONJ biotropia-163 67 14 3 3 NUM biotropia-163 67 15 ' ' CCONJ biotropia-163 67 16 conserved conserved ADJ biotropia-163 67 17 segment segment NOUN biotropia-163 67 18 but but CCONJ biotropia-163 67 19 no no DET biotropia-163 67 20 inserted insert VERB biotropia-163 67 21 gene gene NOUN biotropia-163 67 22 between between ADP biotropia-163 67 23 the the DET biotropia-163 67 24 conserved conserved ADJ biotropia-163 67 25 segments segment NOUN biotropia-163 67 26 , , PUNCT biotropia-163 67 27 there there PRON biotropia-163 67 28 is be VERB biotropia-163 67 29 no no DET biotropia-163 67 30 59 59 NUM biotropia-163 67 31 - - PUNCT biotropia-163 67 32 base base NOUN biotropia-163 67 33 element element NOUN biotropia-163 67 34 ( ( PUNCT biotropia-163 67 35 levesque levesque PROPN biotropia-163 67 36 et et PROPN biotropia-163 67 37 al al PROPN biotropia-163 67 38 . . PROPN biotropia-163 67 39 1995 1995 NUM biotropia-163 67 40 ) ) PUNCT biotropia-163 67 41 . . PUNCT biotropia-163 68 1 the the DET biotropia-163 68 2 other other ADJ biotropia-163 68 3 isolates isolate NOUN biotropia-163 68 4 used use VERB biotropia-163 68 5 in in ADP biotropia-163 68 6 this this DET biotropia-163 68 7 study study NOUN biotropia-163 68 8 were be AUX biotropia-163 68 9 found find VERB biotropia-163 68 10 to to PART biotropia-163 68 11 be be AUX biotropia-163 68 12 similar similar ADJ biotropia-163 68 13 to to ADP biotropia-163 68 14 pvsi pvsi NOUN biotropia-163 68 15 ( ( PUNCT biotropia-163 68 16 table table NOUN biotropia-163 68 17 2 2 NUM biotropia-163 68 18 ) ) PUNCT biotropia-163 68 19 . . PUNCT biotropia-163 69 1 it it PRON biotropia-163 69 2 was be AUX biotropia-163 69 3 reported report VERB biotropia-163 69 4 that that SCONJ biotropia-163 69 5 pvsi pvsi NOUN biotropia-163 69 6 , , PUNCT biotropia-163 69 7 a a DET biotropia-163 69 8 plasmid plasmid NOUN biotropia-163 69 9 derived derive VERB biotropia-163 69 10 from from ADP biotropia-163 69 11 pseudomonas pseudomona NOUN biotropia-163 69 12 aeruginosa aeruginosa NOUN biotropia-163 69 13 , , PUNCT biotropia-163 69 14 possessed possess VERB biotropia-163 69 15 integron integron PROPN biotropia-163 69 16 type type PROPN biotropia-163 69 17 ino ino NOUN biotropia-163 69 18 which which PRON biotropia-163 69 19 has have VERB biotropia-163 69 20 an an DET biotropia-163 69 21 unoccupied unoccupied ADJ biotropia-163 69 22 integration integration NOUN biotropia-163 69 23 site site NOUN biotropia-163 69 24 and and CCONJ biotropia-163 69 25 hence hence ADV biotropia-163 69 26 may may AUX biotropia-163 69 27 be be AUX biotropia-163 69 28 an an DET biotropia-163 69 29 ancestor ancestor NOUN biotropia-163 69 30 of of ADP biotropia-163 69 31 the the DET biotropia-163 69 32 more more ADV biotropia-163 69 33 complex complex ADJ biotropia-163 69 34 integrons integron NOUN biotropia-163 69 35 ( ( PUNCT biotropia-163 69 36 bissonnette bissonnette NOUN biotropia-163 69 37 and and CCONJ biotropia-163 69 38 roy roy PROPN biotropia-163 69 39 1992 1992 NUM biotropia-163 69 40 ) ) PUNCT biotropia-163 69 41 . . PUNCT biotropia-163 70 1 cloning cloning NOUN biotropia-163 70 2 of of ADP biotropia-163 70 3 sti sti PROPN biotropia-163 70 4 in in ADP biotropia-163 70 5 pas900 pas900 PROPN biotropia-163 70 6 ( ( PUNCT biotropia-163 70 7 kmr kmr PROPN biotropia-163 70 8 ) ) PUNCT biotropia-163 70 9 vector vector NOUN biotropia-163 70 10 yielded yield VERB biotropia-163 70 11 recombinant recombinant ADJ biotropia-163 70 12 plasmid plasmid NOUN biotropia-163 70 13 called call VERB biotropia-163 70 14 pcras pcra NOUN biotropia-163 70 15 . . PUNCT biotropia-163 71 1 the the DET biotropia-163 71 2 purpose purpose NOUN biotropia-163 71 3 of of ADP biotropia-163 71 4 cloning cloning NOUN biotropia-163 71 5 was be AUX biotropia-163 71 6 to to PART biotropia-163 71 7 detect detect VERB biotropia-163 71 8 ampicillin ampicillin NOUN biotropia-163 71 9 - - PUNCT biotropia-163 71 10 resistant resistant ADJ biotropia-163 71 11 gene gene NOUN biotropia-163 71 12 in in ADP biotropia-163 71 13 sti sti PROPN biotropia-163 71 14 . . PUNCT biotropia-163 72 1 e. e. PROPN biotropia-163 72 2 coli coli PROPN biotropia-163 72 3 top top VERB biotropia-163 72 4 10 10 NUM biotropia-163 72 5 harbouring harbour VERB biotropia-163 72 6 the the DET biotropia-163 72 7 recombinant recombinant ADJ biotropia-163 72 8 plasmid plasmid ADJ biotropia-163 72 9 pcras pcra NOUN biotropia-163 72 10 was be AUX biotropia-163 72 11 sensitive sensitive ADJ biotropia-163 72 12 to to ADP biotropia-163 72 13 ampicillin ampicillin PROPN biotropia-163 72 14 . . PUNCT biotropia-163 73 1 this this DET biotropia-163 73 2 result result NOUN biotropia-163 73 3 indicated indicate VERB biotropia-163 73 4 that that SCONJ biotropia-163 73 5 sti sti PROPN biotropia-163 73 6 was be AUX biotropia-163 73 7 not not PART biotropia-163 73 8 an an DET biotropia-163 73 9 ampicillin ampicillin NOUN biotropia-163 73 10 - - PUNCT biotropia-163 73 11 resistant resistant ADJ biotropia-163 73 12 gene gene NOUN biotropia-163 73 13 , , PUNCT biotropia-163 73 14 or or CCONJ biotropia-163 73 15 it it PRON biotropia-163 73 16 was be AUX biotropia-163 73 17 an an DET biotropia-163 73 18 ampicillin ampicillin NOUN biotropia-163 73 19 - - PUNCT biotropia-163 73 20 resistant resistant ADJ biotropia-163 73 21 gene gene NOUN biotropia-163 73 22 which which PRON biotropia-163 73 23 could could AUX biotropia-163 73 24 not not PART biotropia-163 73 25 be be AUX biotropia-163 73 26 expressed express VERB biotropia-163 73 27 . . PUNCT biotropia-163 74 1 expression expression NOUN biotropia-163 74 2 of of ADP biotropia-163 74 3 antibiotic antibiotic ADJ biotropia-163 74 4 - - PUNCT biotropia-163 74 5 resistant resistant ADJ biotropia-163 74 6 genes gene NOUN biotropia-163 74 7 in in ADP biotropia-163 74 8 the the DET biotropia-163 74 9 integrated integrate VERB biotropia-163 74 10 cassettes cassette NOUN biotropia-163 74 11 of of ADP biotropia-163 74 12 integrons integron NOUN biotropia-163 74 13 depends depend VERB biotropia-163 74 14 on on ADP biotropia-163 74 15 cassette cassette NOUN biotropia-163 74 16 position position NOUN biotropia-163 74 17 . . PUNCT biotropia-163 75 1 in in ADP biotropia-163 75 2 all all DET biotropia-163 75 3 cases case NOUN biotropia-163 75 4 , , PUNCT biotropia-163 75 5 the the DET biotropia-163 75 6 resistance resistance NOUN biotropia-163 75 7 level level NOUN biotropia-163 75 8 was be AUX biotropia-163 75 9 the the DET biotropia-163 75 10 highest high ADJ biotropia-163 75 11 when when SCONJ biotropia-163 75 12 the the DET biotropia-163 75 13 gene gene NOUN biotropia-163 75 14 was be AUX biotropia-163 75 15 present present ADJ biotropia-163 75 16 in in ADP biotropia-163 75 17 the the DET biotropia-163 75 18 first first ADJ biotropia-163 75 19 cassette cassette NOUN biotropia-163 75 20 ( ( PUNCT biotropia-163 75 21 collis colli NOUN biotropia-163 75 22 and and CCONJ biotropia-163 75 23 hall hall NOUN biotropia-163 75 24 1995 1995 NUM biotropia-163 75 25 ) ) PUNCT biotropia-163 75 26 . . PUNCT biotropia-163 76 1 therefore therefore ADV biotropia-163 76 2 , , PUNCT biotropia-163 76 3 there there PRON biotropia-163 76 4 is be VERB biotropia-163 76 5 a a DET biotropia-163 76 6 need need NOUN biotropia-163 76 7 to to PART biotropia-163 76 8 sequence sequence VERB biotropia-163 76 9 the the DET biotropia-163 76 10 sti sti PROPN biotropia-163 76 11 for for ADP biotropia-163 76 12 gene gene NOUN biotropia-163 76 13 characterization characterization NOUN biotropia-163 76 14 . . PUNCT biotropia-163 77 1 14 14 NUM biotropia-163 77 2 biotroopia biotroopia ADJ biotropia-163 77 3 no no NOUN biotropia-163 77 4 . . PROPN biotropia-163 77 5 15 15 NUM biotropia-163 77 6 , , PUNCT biotropia-163 77 7 2001 2001 NUM biotropia-163 77 8 figure figure NOUN biotropia-163 77 9 2 2 NUM biotropia-163 77 10 . . PUNCT biotropia-163 77 11 integron integron PROPN biotropia-163 77 12 pcr pcr PROPN biotropia-163 77 13 product product NOUN biotropia-163 77 14 from from ADP biotropia-163 77 15 total total ADJ biotropia-163 77 16 dna dna PROPN biotropia-163 77 17 of of ADP biotropia-163 77 18 pvhal pvhal NOUN biotropia-163 77 19 isolate isolate PROPN biotropia-163 77 20 ( ( PUNCT biotropia-163 77 21 lane lane NOUN biotropia-163 77 22 1 1 NUM biotropia-163 77 23 ) ) PUNCT biotropia-163 77 24 , , PUNCT biotropia-163 77 25 sg2 sg2 PROPN biotropia-163 77 26 isolate isolate PROPN biotropia-163 77 27 ( ( PUNCT biotropia-163 77 28 lane lane NOUN biotropia-163 77 29 3 3 NUM biotropia-163 77 30 ) ) PUNCT biotropia-163 77 31 , , PUNCT biotropia-163 77 32 and and CCONJ biotropia-163 77 33 kb kb PROPN biotropia-163 77 34 ladder ladder NOUN biotropia-163 77 35 as as ADP biotropia-163 77 36 marker marker NOUN biotropia-163 77 37 ( ( PUNCT biotropia-163 77 38 lane lane NOUN biotropia-163 77 39 2 2 NUM biotropia-163 77 40 ) ) PUNCT biotropia-163 77 41 . . PUNCT biotropia-163 78 1 15 15 NUM biotropia-163 78 2 a a DET biotropia-163 78 3 novel novel ADJ biotropia-163 78 4 integron integron NOUN biotropia-163 78 5 in in ADP biotropia-163 78 6 the the DET biotropia-163 78 7 genome genome NOUN biotropia-163 78 8 of of ADP biotropia-163 78 9 escherichia escherichia NOUN biotropia-163 78 10 coli coli NOUN biotropia-163 78 11 — — PUNCT biotropia-163 78 12 esti esti PROPN biotropia-163 78 13 puspitasari puspitasari PROPN biotropia-163 78 14 et et PROPN biotropia-163 78 15 al al PROPN biotropia-163 78 16 . . PUNCT biotropia-163 79 1 sequencing sequence VERB biotropia-163 79 2 cloning cloning NOUN biotropia-163 79 3 of of ADP biotropia-163 79 4 sti sti PROPN biotropia-163 79 5 in in ADP biotropia-163 79 6 pgem pgem PROPN biotropia-163 79 7 - - PUNCT biotropia-163 79 8 t t NOUN biotropia-163 79 9 easy easy ADJ biotropia-163 79 10 vector vector NOUN biotropia-163 79 11 yielded yield VERB biotropia-163 79 12 recombinant recombinant ADJ biotropia-163 79 13 plasmid plasmid NOUN biotropia-163 79 14 designated designate VERB biotropia-163 79 15 as as SCONJ biotropia-163 79 16 ps2 ps2 PROPN biotropia-163 79 17 t. t. PROPN biotropia-163 79 18 transformant transformant PROPN biotropia-163 79 19 was be AUX biotropia-163 79 20 isolated isolate VERB biotropia-163 79 21 , , PUNCT biotropia-163 79 22 and and CCONJ biotropia-163 79 23 then then ADV biotropia-163 79 24 sequenced sequence VERB biotropia-163 79 25 . . PUNCT biotropia-163 80 1 the the DET biotropia-163 80 2 result result NOUN biotropia-163 80 3 indicated indicate VERB biotropia-163 80 4 that that SCONJ biotropia-163 80 5 the the DET biotropia-163 80 6 sti sti PROPN biotropia-163 80 7 sequence sequence NOUN biotropia-163 80 8 was be AUX biotropia-163 80 9 homologous homologous ADJ biotropia-163 80 10 to to ADP biotropia-163 80 11 aada aada NOUN biotropia-163 80 12 gene gene NOUN biotropia-163 80 13 encoding encode VERB biotropia-163 80 14 aminoglycoside aminoglycoside ADV biotropia-163 80 15 adenyltransferase adenyltransferase NOUN biotropia-163 80 16 , , PUNCT biotropia-163 80 17 which which PRON biotropia-163 80 18 was be AUX biotropia-163 80 19 responsible responsible ADJ biotropia-163 80 20 for for ADP biotropia-163 80 21 streptomycin streptomycin PROPN biotropia-163 80 22 and and CCONJ biotropia-163 80 23 spectinomycin spectinomycin PROPN biotropia-163 80 24 resistance resistance NOUN biotropia-163 80 25 in in ADP biotropia-163 80 26 e. e. PROPN biotropia-163 80 27 coli coli PROPN biotropia-163 80 28 . . PUNCT biotropia-163 81 1 blast blast NOUN biotropia-163 81 2 search search NOUN biotropia-163 81 3 analysis analysis NOUN biotropia-163 81 4 of of ADP biotropia-163 81 5 the the DET biotropia-163 81 6 sti sti PROPN biotropia-163 81 7 sequence sequence NOUN biotropia-163 81 8 indicated indicate VERB biotropia-163 81 9 that that SCONJ biotropia-163 81 10 the the DET biotropia-163 81 11 700 700 NUM biotropia-163 81 12 nucleotides nucleotide NOUN biotropia-163 81 13 of of ADP biotropia-163 81 14 3'sti 3'sti NUM biotropia-163 81 15 are be AUX biotropia-163 81 16 integron integron NOUN biotropia-163 81 17 type type NOUN biotropia-163 81 18 3 3 NUM biotropia-163 81 19 ( ( PUNCT biotropia-163 81 20 int int NOUN biotropia-163 81 21 3 3 NUM biotropia-163 81 22 ) ) PUNCT biotropia-163 81 23 as as ADP biotropia-163 81 24 in in ADP biotropia-163 81 25 the the DET biotropia-163 81 26 incl incl NOUN biotropia-163 81 27 / / SYM biotropia-163 81 28 m m NOUN biotropia-163 81 29 plasmid plasmid ADJ biotropia-163 81 30 from from ADP biotropia-163 81 31 salmonella salmonella NOUN biotropia-163 81 32 typhimurium typhimurium NOUN biotropia-163 81 33 ( ( PUNCT biotropia-163 81 34 96 96 NUM biotropia-163 81 35 % % NOUN biotropia-163 81 36 identity identity NOUN biotropia-163 81 37 ) ) PUNCT biotropia-163 81 38 and and CCONJ biotropia-163 81 39 pnccsol pnccsol VERB biotropia-163 81 40 plasmid plasmid NOUN biotropia-163 81 41 from from ADP biotropia-163 81 42 enterococcus enterococcus NOUN biotropia-163 81 43 faecalis faecali NOUN biotropia-163 81 44 ( ( PUNCT biotropia-163 81 45 96 96 NUM biotropia-163 81 46 % % NOUN biotropia-163 81 47 identity identity NOUN biotropia-163 81 48 ) ) PUNCT biotropia-163 81 49 . . PUNCT biotropia-163 82 1 the the DET biotropia-163 82 2 other other ADJ biotropia-163 82 3 600 600 NUM biotropia-163 82 4 nucleotides nucleotide NOUN biotropia-163 82 5 of of ADP biotropia-163 82 6 5'sti 5'sti NUM biotropia-163 82 7 were be AUX biotropia-163 82 8 similar similar ADJ biotropia-163 82 9 to to ADP biotropia-163 82 10 the the DET biotropia-163 82 11 pnccsol pnccsol NOUN biotropia-163 82 12 plasmid plasmid NOUN biotropia-163 82 13 from from ADP biotropia-163 82 14 e e NOUN biotropia-163 82 15 , , PUNCT biotropia-163 82 16 faecalis faecali NOUN biotropia-163 82 17 ( ( PUNCT biotropia-163 82 18 97 97 NUM biotropia-163 82 19 % % NOUN biotropia-163 82 20 identity identity NOUN biotropia-163 82 21 ) ) PUNCT biotropia-163 82 22 and and CCONJ biotropia-163 82 23 the the DET biotropia-163 82 24 r100.1 r100.1 NOUN biotropia-163 82 25 plasmid plasmid NOUN biotropia-163 82 26 from from ADP biotropia-163 82 27 bacteriophage bacteriophage NOUN biotropia-163 82 28 t4 t4 PROPN biotropia-163 82 29 ( ( PUNCT biotropia-163 82 30 97 97 NUM biotropia-163 82 31 % % NOUN biotropia-163 82 32 identity identity NOUN biotropia-163 82 33 ) ) PUNCT biotropia-163 82 34 . . PUNCT biotropia-163 83 1 in in ADP biotropia-163 83 2 summary summary NOUN biotropia-163 83 3 , , PUNCT biotropia-163 83 4 there there PRON biotropia-163 83 5 were be VERB biotropia-163 83 6 eight eight NUM biotropia-163 83 7 isolates isolate NOUN biotropia-163 83 8 out out ADP biotropia-163 83 9 of of ADP biotropia-163 83 10 nine nine NUM biotropia-163 83 11 e. e. PROPN biotropia-163 83 12 coli coli NOUN biotropia-163 83 13 isolates isolate NOUN biotropia-163 83 14 possessing possess VERB biotropia-163 83 15 a a DET biotropia-163 83 16 conserved conserved ADJ biotropia-163 83 17 segment segment NOUN biotropia-163 83 18 of of ADP biotropia-163 83 19 integron integron NOUN biotropia-163 83 20 and and CCONJ biotropia-163 83 21 only only ADV biotropia-163 83 22 one one NUM biotropia-163 83 23 possessed possess VERB biotropia-163 83 24 inserted insert VERB biotropia-163 83 25 gene gene NOUN biotropia-163 83 26 between between ADP biotropia-163 83 27 the the DET biotropia-163 83 28 conserved conserve VERB biotropia-163 83 29 segments segment NOUN biotropia-163 83 30 of of ADP biotropia-163 83 31 the the DET biotropia-163 83 32 integron integron NOUN biotropia-163 83 33 . . PUNCT biotropia-163 84 1 the the DET biotropia-163 84 2 sequencing sequence VERB biotropia-163 84 3 analysis analysis NOUN biotropia-163 84 4 indicated indicate VERB biotropia-163 84 5 that that SCONJ biotropia-163 84 6 spectinomycinand spectinomycinand NOUN biotropia-163 84 7 streptomycin streptomycin NOUN biotropia-163 84 8 - - PUNCT biotropia-163 84 9 resistant resistant ADJ biotropia-163 84 10 gene gene NOUN biotropia-163 84 11 was be AUX biotropia-163 84 12 present present ADJ biotropia-163 84 13 in in ADP biotropia-163 84 14 the the DET biotropia-163 84 15 1 1 NUM biotropia-163 84 16 - - PUNCT biotropia-163 84 17 kb kb PROPN biotropia-163 84 18 dna dna PROPN biotropia-163 84 19 fragment fragment NOUN biotropia-163 84 20 . . PUNCT biotropia-163 85 1 acknowledgments acknowledgment NOUN biotropia-163 85 2 this this DET biotropia-163 85 3 work work NOUN biotropia-163 85 4 was be AUX biotropia-163 85 5 supported support VERB biotropia-163 85 6 by by ADP biotropia-163 85 7 dip dip NOUN biotropia-163 85 8 project project NOUN biotropia-163 85 9 for for ADP biotropia-163 85 10 biotrop biotrop NOUN biotropia-163 85 11 1999 1999 NUM biotropia-163 85 12 / / SYM biotropia-163 85 13 2000 2000 NUM biotropia-163 85 14 to to ADP biotropia-163 85 15 antonius antonius PROPN biotropia-163 85 16 suwanto suwanto NOUN biotropia-163 85 17 . . PUNCT biotropia-163 86 1 we we PRON biotropia-163 86 2 thank thank VERB biotropia-163 86 3 yeo yeo PROPN biotropia-163 86 4 chew chew PROPN biotropia-163 86 5 chieng chieng PROPN biotropia-163 86 6 from from ADP biotropia-163 86 7 department department PROPN biotropia-163 86 8 of of ADP biotropia-163 86 9 microbiology microbiology NOUN biotropia-163 86 10 , , PUNCT biotropia-163 86 11 national national ADJ biotropia-163 86 12 university university PROPN biotropia-163 86 13 of of ADP biotropia-163 86 14 singapore singapore PROPN biotropia-163 86 15 for for ADP biotropia-163 86 16 conducting conduct VERB biotropia-163 86 17 dna dna NOUN biotropia-163 86 18 sequencing sequencing NOUN biotropia-163 86 19 . . PUNCT biotropia-163 87 1 references reference NOUN biotropia-163 87 2 auffenberg auffenberg PROPN biotropia-163 87 3 , , PUNCT biotropia-163 87 4 w. w. NOUN biotropia-163 87 5 1981.the 1981.the PRON biotropia-163 87 6 behavioral behavioral ADJ biotropia-163 87 7 ecology ecology NOUN biotropia-163 87 8 of of ADP biotropia-163 87 9 the the DET biotropia-163 87 10 komodo komodo PROPN biotropia-163 87 11 monitor monitor NOUN biotropia-163 87 12 . . PUNCT biotropia-163 88 1 university university NOUN biotropia-163 88 2 press press NOUN biotropia-163 88 3 of of ADP biotropia-163 88 4 florida florida PROPN biotropia-163 88 5 , , PUNCT biotropia-163 88 6 florida florida PROPN biotropia-163 88 7 bissonnette bissonnette PROPN biotropia-163 88 8 , , PUNCT biotropia-163 88 9 l. l. PROPN biotropia-163 88 10 and and CCONJ biotropia-163 88 11 p.h p.h PROPN biotropia-163 88 12 . . PROPN biotropia-163 88 13 roy roy PROPN biotropia-163 88 14 . . PROPN biotropia-163 88 15 1992 1992 NUM biotropia-163 88 16 . . PUNCT biotropia-163 89 1 characterization characterization NOUN biotropia-163 89 2 of of ADP biotropia-163 89 3 ino ino NOUN biotropia-163 89 4 of of ADP biotropia-163 89 5 pseudomonas pseudomona NOUN biotropia-163 89 6 aeruginosa aeruginosa NOUN biotropia-163 89 7 plasmid plasmid ADP biotropia-163 89 8 pvsl pvsl PROPN biotropia-163 89 9 , , PUNCT biotropia-163 89 10 an an DET biotropia-163 89 11 ancestor ancestor NOUN biotropia-163 89 12 of of ADP biotropia-163 89 13 integrons integron NOUN biotropia-163 89 14 of of ADP biotropia-163 89 15 multiresistance multiresistance NOUN biotropia-163 89 16 plasmids plasmid NOUN biotropia-163 89 17 and and CCONJ biotropia-163 89 18 transposons transposon NOUN biotropia-163 89 19 of of ADP biotropia-163 89 20 gram gram NOUN biotropia-163 89 21 - - PUNCT biotropia-163 89 22 negative negative ADJ biotropia-163 89 23 bacteria bacteria NOUN biotropia-163 89 24 . . PUNCT biotropia-163 90 1 j. j. PROPN biotropia-163 90 2 bacteriol bacteriol PROPN biotropia-163 90 3 . . PUNCT biotropia-163 91 1 174:1248 174:1248 NUM biotropia-163 91 2 - - SYM biotropia-163 91 3 1257 1257 NUM biotropia-163 91 4 . . PUNCT biotropia-163 92 1 collis collis PROPN biotropia-163 92 2 , , PUNCT biotropia-163 92 3 c.m c.m PROPN biotropia-163 92 4 . . PROPN biotropia-163 92 5 and and CCONJ biotropia-163 92 6 r.m.hall r.m.hall NOUN biotropia-163 92 7 . . PUNCT biotropia-163 92 8 1995 1995 NUM biotropia-163 92 9 . . PUNCT biotropia-163 93 1 expression expression NOUN biotropia-163 93 2 of of ADP biotropia-163 93 3 antibiotic antibiotic ADJ biotropia-163 93 4 resistance resistance NOUN biotropia-163 93 5 genes gene NOUN biotropia-163 93 6 in in ADP biotropia-163 93 7 the the DET biotropia-163 93 8 integrated integrate VERB biotropia-163 93 9 cassettes cassette NOUN biotropia-163 93 10 of of ADP biotropia-163 93 11 integrons integron NOUN biotropia-163 93 12 . . PUNCT biotropia-163 94 1 antimicrob antimicrob PROPN biotropia-163 94 2 . . PUNCT biotropia-163 95 1 agents agent NOUN biotropia-163 95 2 . . PUNCT biotropia-163 96 1 chemother chemother PROPN biotropia-163 96 2 . . PUNCT biotropia-163 97 1 39 39 NUM biotropia-163 97 2 : : PUNCT biotropia-163 97 3 155 155 NUM biotropia-163 97 4 - - SYM biotropia-163 97 5 162 162 NUM biotropia-163 97 6 . . PUNCT biotropia-163 98 1 desselberger desselberger ADV biotropia-163 98 2 , , PUNCT biotropia-163 98 3 u. u. NOUN biotropia-163 98 4 1998 1998 NUM biotropia-163 98 5 . . PUNCT biotropia-163 99 1 resistance resistance NOUN biotropia-163 99 2 to to ADP biotropia-163 99 3 antibiotics antibiotic NOUN biotropia-163 99 4 and and CCONJ biotropia-163 99 5 other other ADJ biotropia-163 99 6 antimicrobiol antimicrobiol NOUN biotropia-163 99 7 agents agent NOUN biotropia-163 99 8 . . PUNCT biotropia-163 100 1 sgm sgm PROPN biotropia-163 100 2 quarterly quarterly PROPN biotropia-163 100 3 . . PUNCT biotropia-163 101 1 25:94 25:94 NUM biotropia-163 101 2 - - SYM biotropia-163 101 3 95 95 NUM biotropia-163 101 4 . . PUNCT biotropia-163 102 1 francia francia PROPN biotropia-163 102 2 , , PUNCT biotropia-163 102 3 mv mv PROPN biotropia-163 102 4 . . PROPN biotropia-163 102 5 , , PUNCT biotropia-163 102 6 j.c j.c PROPN biotropia-163 102 7 . . PROPN biotropia-163 102 8 zabala zabala PROPN biotropia-163 102 9 , , PUNCT biotropia-163 102 10 f. f. PROPN biotropia-163 102 11 cruz cruz PROPN biotropia-163 102 12 , , PUNCT biotropia-163 102 13 j.m.g j.m.g PROPN biotropia-163 102 14 . . PUNCT biotropia-163 103 1 lobo lobo PROPN biotropia-163 103 2 . . PUNCT biotropia-163 104 1 1999 1999 NUM biotropia-163 104 2 . . PUNCT biotropia-163 105 1 the the DET biotropia-163 105 2 intl intl PROPN biotropia-163 105 3 integron integron PROPN biotropia-163 105 4 integrase integrase PROPN biotropia-163 105 5 preferentially preferentially ADV biotropia-163 105 6 binds bind VERB biotropia-163 105 7 single single ADJ biotropia-163 105 8 - - PUNCT biotropia-163 105 9 stranded strand VERB biotropia-163 105 10 dna dna NOUN biotropia-163 105 11 of of ADP biotropia-163 105 12 the the DET biotropia-163 105 13 attc attc PROPN biotropia-163 105 14 site site NOUN biotropia-163 105 15 . . PUNCT biotropia-163 106 1 j. j. PROPN biotropia-163 106 2 bacteriol bacteriol PROPN biotropia-163 106 3 . . PUNCT biotropia-163 107 1 181 181 NUM biotropia-163 107 2 : : PUNCT biotropia-163 107 3 6844 6844 NUM biotropia-163 107 4 - - SYM biotropia-163 107 5 6849 6849 NUM biotropia-163 107 6 . . PUNCT biotropia-163 108 1 gian gian PROPN biotropia-163 108 2 , , PUNCT biotropia-163 108 3 g.s g.s PROPN biotropia-163 108 4 . . PROPN biotropia-163 108 5 dan dan PROPN biotropia-163 108 6 v.h.s.gian v.h.s.gian PROPN biotropia-163 108 7 . . PROPN biotropia-163 108 8 1995 1995 NUM biotropia-163 108 9 . . PUNCT biotropia-163 109 1 farmakologi farmakologi PROPN biotropia-163 109 2 dan dan PROPN biotropia-163 109 3 terapi terapi PROPN biotropia-163 109 4 : : PUNCT biotropia-163 109 5 aminoglikosid aminoglikosid PROPN biotropia-163 109 6 . . PUNCT biotropia-163 110 1 ed ed AUX biotropia-163 110 2 . . PUNCT biotropia-163 110 3 ke-4 ke-4 PROPN biotropia-163 110 4 . . PUNCT biotropia-163 111 1 gaya gaya PROPN biotropia-163 111 2 baru baru PROPN biotropia-163 111 3 , , PUNCT biotropia-163 111 4 jakarta jakarta PROPN biotropia-163 111 5 . . PUNCT biotropia-163 112 1 jacoby jacoby PROPN biotropia-163 112 2 , , PUNCT biotropia-163 112 3 g. g. PROPN biotropia-163 112 4 1992 1992 NUM biotropia-163 112 5 . . PUNCT biotropia-163 113 1 exploring explore VERB biotropia-163 113 2 new new ADJ biotropia-163 113 3 strategies strategy NOUN biotropia-163 113 4 to to PART biotropia-163 113 5 fight fight VERB biotropia-163 113 6 drug drug NOUN biotropia-163 113 7 - - PUNCT biotropia-163 113 8 resistant resistant ADJ biotropia-163 113 9 microbes microbe NOUN biotropia-163 113 10 . . PUNCT biotropia-163 114 1 science science NOUN biotropia-163 114 2 . . PUNCT biotropia-163 115 1 257:1036 257:1036 NUM biotropia-163 115 2 - - SYM biotropia-163 115 3 1038 1038 NUM biotropia-163 115 4 . . PUNCT biotropia-163 116 1 levesque levesque PROPN biotropia-163 116 2 , , PUNCT biotropia-163 116 3 c. c. PROPN biotropia-163 116 4 , , PUNCT biotropia-163 116 5 l. l. PROPN biotropia-163 116 6 piche piche PROPN biotropia-163 116 7 , , PUNCT biotropia-163 116 8 c. c. PROPN biotropia-163 116 9 larose larose PROPN biotropia-163 116 10 , , PUNCT biotropia-163 116 11 and and CCONJ biotropia-163 116 12 p.h p.h PROPN biotropia-163 116 13 . . PROPN biotropia-163 116 14 roy roy PROPN biotropia-163 116 15 . . PROPN biotropia-163 116 16 1995 1995 NUM biotropia-163 116 17 . . PUNCT biotropia-163 117 1 pcr pcr ADJ biotropia-163 117 2 mapping mapping NOUN biotropia-163 117 3 of of ADP biotropia-163 117 4 integrons integron NOUN biotropia-163 117 5 reveals reveal VERB biotropia-163 117 6 several several ADJ biotropia-163 117 7 novel novel ADJ biotropia-163 117 8 combinations combination NOUN biotropia-163 117 9 of of ADP biotropia-163 117 10 resistant resistant ADJ biotropia-163 117 11 genes gene NOUN biotropia-163 117 12 . . PUNCT biotropia-163 118 1 antimicrob antimicrob ADJ biotropia-163 118 2 . . PUNCT biotropia-163 118 3 agents agent NOUN biotropia-163 118 4 . . PUNCT biotropia-163 119 1 chemother chemother PROPN biotropia-163 119 2 . . PUNCT biotropia-163 120 1 39:185 39:185 NUM biotropia-163 120 2 - - SYM biotropia-163 120 3 191 191 NUM biotropia-163 120 4 . . PUNCT biotropia-163 121 1 16 16 NUM biotropia-163 121 2 biotropia biotropia NOUN biotropia-163 121 3 no no NOUN biotropia-163 121 4 . . PROPN biotropia-163 121 5 16 16 NUM biotropia-163 121 6 , , PUNCT biotropia-163 121 7 2001 2001 NUM biotropia-163 121 8 madigan madigan X biotropia-163 121 9 , , PUNCT biotropia-163 121 10 m.t m.t PROPN biotropia-163 121 11 . . PROPN biotropia-163 121 12 , , PUNCT biotropia-163 121 13 j.m j.m PROPN biotropia-163 121 14 . . PROPN biotropia-163 121 15 martinko martinko PROPN biotropia-163 121 16 , , PUNCT biotropia-163 121 17 and and CCONJ biotropia-163 121 18 j. j. PROPN biotropia-163 121 19 parker parker PROPN biotropia-163 121 20 , , PUNCT biotropia-163 121 21 1997 1997 NUM biotropia-163 121 22 . . PUNCT biotropia-163 122 1 biology biology NOUN biotropia-163 122 2 of of ADP biotropia-163 122 3 microorganisms microorganism NOUN biotropia-163 122 4 . . PUNCT biotropia-163 123 1 8"1 8"1 NUM biotropia-163 123 2 ed ed NOUN biotropia-163 123 3 . . PUNCT biotropia-163 123 4 prentice prentice PROPN biotropia-163 123 5 hall hall PROPN biotropia-163 123 6 , , PUNCT biotropia-163 123 7 new new PROPN biotropia-163 123 8 jersey jersey PROPN biotropia-163 123 9 . . PUNCT biotropia-163 124 1 neu neu PROPN biotropia-163 124 2 , , PUNCT biotropia-163 124 3 h.c h.c PROPN biotropia-163 124 4 . . PROPN biotropia-163 124 5 1992 1992 NUM biotropia-163 124 6 . . PUNCT biotropia-163 125 1 the the DET biotropia-163 125 2 crisis crisis NOUN biotropia-163 125 3 in in ADP biotropia-163 125 4 antibiotic antibiotic ADJ biotropia-163 125 5 resistance resistance NOUN biotropia-163 125 6 . . PUNCT biotropia-163 126 1 science science NOUN biotropia-163 126 2 . . PUNCT biotropia-163 127 1 257:1064 257:1064 NUM biotropia-163 127 2 - - SYM biotropia-163 127 3 1072 1072 NUM biotropia-163 127 4 . . PUNCT biotropia-163 128 1 sambrook sambrook PROPN biotropia-163 128 2 , , PUNCT biotropia-163 128 3 j. j. PROPN biotropia-163 128 4 , , PUNCT biotropia-163 128 5 e.k e.k PROPN biotropia-163 128 6 . . PROPN biotropia-163 128 7 frisch frisch PROPN biotropia-163 128 8 , , PUNCT biotropia-163 128 9 t. t. PROPN biotropia-163 128 10 maniatis maniatis PROPN biotropia-163 128 11 . . PUNCT biotropia-163 128 12 1989 1989 NUM biotropia-163 128 13 . . PUNCT biotropia-163 129 1 molecular molecular ADJ biotropia-163 129 2 cloning cloning NOUN biotropia-163 129 3 , , PUNCT biotropia-163 129 4 a a DET biotropia-163 129 5 laboratory laboratory NOUN biotropia-163 129 6 manual manual NOUN biotropia-163 129 7 . . PUNCT biotropia-163 130 1 ed ed NOUN biotropia-163 130 2 . . PUNCT biotropia-163 131 1 ke-2 ke-2 X biotropia-163 131 2 . . PUNCT biotropia-163 132 1 cold cold ADJ biotropia-163 132 2 spring spring NOUN biotropia-163 132 3 harbor harbor PROPN biotropia-163 132 4 laboratory laboratory PROPN biotropia-163 132 5 press press PROPN biotropia-163 132 6 . . PUNCT biotropia-163 133 1 cold cold ADJ biotropia-163 133 2 spring spring NOUN biotropia-163 133 3 harbor harbor PROPN biotropia-163 133 4 , , PUNCT biotropia-163 133 5 usa usa PROPN biotropia-163 133 6 . . PROPN biotropia-163 133 7 schumm schumm PROPN biotropia-163 133 8 , , PUNCT biotropia-163 133 9 d.e d.e PROPN biotropia-163 133 10 . . PROPN biotropia-163 133 11 1992 1992 NUM biotropia-163 133 12 . . PUNCT biotropia-163 134 1 intisari intisari ADJ biotropia-163 134 2 biokimia biokimia NOUN biotropia-163 134 3 . . PUNCT biotropia-163 135 1 terjemahan terjemahan PROPN biotropia-163 135 2 moch moch PROPN biotropia-163 135 3 . . PUNCT biotropia-163 136 1 sadikin sadikin PROPN biotropia-163 136 2 . . PUNCT biotropia-163 137 1 binarupa binarupa PROPN biotropia-163 137 2 aksara aksara PROPN biotropia-163 137 3 , , PUNCT biotropia-163 137 4 jakarta jakarta PROPN biotropia-163 137 5 . . PUNCT biotropia-163 138 1 yogiara yogiara NOUN biotropia-163 138 2 . . PUNCT biotropia-163 139 1 1998 1998 NUM biotropia-163 139 2 . . PUNCT biotropia-163 140 1 keragaman keragaman PROPN biotropia-163 140 2 genetik genetik PROPN biotropia-163 140 3 isolat isolat NOUN biotropia-163 140 4 escherichia escherichia NOUN biotropia-163 140 5 coli coli NOUN biotropia-163 140 6 dari dari PROPN biotropia-163 140 7 rongga rongga PROPN biotropia-163 140 8 mulut mulut PROPN biotropia-163 140 9 biawak biawak PROPN biotropia-163 140 10 ( ( PUNCT biotropia-163 140 11 varanus varanus PROPN biotropia-163 140 12 ) ) PUNCT biotropia-163 140 13 dan dan PROPN biotropia-163 140 14 kloning klone VERB biotropia-163 140 15 gen gen PROPN biotropia-163 140 16 penyandi penyandi NOUN biotropia-163 140 17 resistensi resistensi NOUN biotropia-163 140 18 ampisilin ampisilin PROPN biotropia-163 140 19 . . PUNCT biotropia-163 141 1 skripsi skripsi PROPN biotropia-163 141 2 . . PUNCT biotropia-163 142 1 jurusan jurusan PROPN biotropia-163 142 2 biologi biologi PROPN biotropia-163 142 3 fmipa fmipa PROPN biotropia-163 142 4 ipb ipb PROPN biotropia-163 142 5 , , PUNCT biotropia-163 142 6 bogor bogor NOUN biotropia-163 142 7 . . PUNCT biotropia-163 143 1 17 17 NUM