id	sid	tid	token	lemma	pos
biotropia-182	1	1	microsoft	microsoft	PROPN
biotropia-182	1	2	word	word	NOUN
biotropia-182	1	3	36	36	NUM
biotropia-182	1	4	biotropia	biotropia	NOUN
biotropia-182	1	5	no	no	NOUN
biotropia-182	1	6	.	.	PROPN
biotropia-182	1	7	20	20	NUM
biotropia-182	1	8	,	,	PUNCT
biotropia-182	1	9	2003	2003	NUM
biotropia-182	1	10	:	:	PUNCT
biotropia-182	1	11	36	36	NUM
biotropia-182	1	12	48	48	NUM
biotropia-182	1	13	heterologous	heterologous	ADJ
biotropia-182	1	14	expression	expression	NOUN
biotropia-182	1	15	of	of	ADP
biotropia-182	1	16	a	a	DET
biotropia-182	1	17	chitinase	chitinase	NOUN
biotropia-182	1	18	gene	gene	NOUN
biotropia-182	1	19	from	from	ADP
biotropia-182	1	20	aeromonas	aeromonas	PROPN
biotropia-182	1	21	caviaein	caviaein	PROPN
biotropia-182	1	22	pseudomonas	pseudomonas	PROPN
biotropia-182	1	23	fluorescens	fluorescens	PROPN
biotropia-182	1	24	amarila	amarila	PROPN
biotropia-182	1	25	mahku'+	mahku'+	PROPN
biotropia-182	1	26	,	,	PUNCT
biotropia-182	1	27	antonius	antonius	NOUN
biotropia-182	1	28	suwanto1'2	suwanto1'2	NOUN
biotropia-182	1	29	'	'	PUNCT
biotropia-182	1	30	"	"	PUNCT
biotropia-182	1	31	,	,	PUNCT
biotropia-182	1	32	budi	budi	PROPN
biotropia-182	1	33	tjahjono3	tjahjono3	PROPN
biotropia-182	1	34	,	,	PUNCT
biotropia-182	1	35	rob	rob	PROPN
biotropia-182	1	36	harling4	harling4	PROPN
biotropia-182	2	1	'	'	PUNCT
biotropia-182	2	2	southeast	southeast	ADJ
biotropia-182	2	3	asian	asian	ADJ
biotropia-182	2	4	regional	regional	ADJ
biotropia-182	2	5	center	center	NOUN
biotropia-182	2	6	for	for	ADP
biotropia-182	2	7	tropical	tropical	ADJ
biotropia-182	2	8	biology	biology	NOUN
biotropia-182	2	9	(	(	PUNCT
biotropia-182	2	10	seameo	seameo	NOUN
biotropia-182	2	11	biotrop	biotrop	NOUN
biotropia-182	2	12	)	)	PUNCT
biotropia-182	2	13	,	,	PUNCT
biotropia-182	2	14	bogor	bogor	NOUN
biotropia-182	2	15	,	,	PUNCT
biotropia-182	2	16	2	2	NUM
biotropia-182	2	17	department	department	NOUN
biotropia-182	2	18	of	of	ADP
biotropia-182	2	19	biology	biology	NOUN
biotropia-182	2	20	,	,	PUNCT
biotropia-182	2	21	faculty	faculty	NOUN
biotropia-182	2	22	of	of	ADP
biotropia-182	2	23	mathematics	mathematic	NOUN
biotropia-182	2	24	and	and	CCONJ
biotropia-182	2	25	sciences	science	NOUN
biotropia-182	2	26	,	,	PUNCT
biotropia-182	2	27	3	3	NUM
biotropia-182	2	28	department	department	NOUN
biotropia-182	2	29	of	of	ADP
biotropia-182	2	30	plant	plant	NOUN
biotropia-182	2	31	pests	pest	NOUN
biotropia-182	2	32	and	and	CCONJ
biotropia-182	2	33	diseases	disease	NOUN
biotropia-182	2	34	,	,	PUNCT
biotropia-182	2	35	faculty	faculty	NOUN
biotropia-182	2	36	of	of	ADP
biotropia-182	2	37	agriculture	agriculture	NOUN
biotropia-182	2	38	,	,	PUNCT
biotropia-182	2	39	bogor	bogor	PROPN
biotropia-182	2	40	agricultural	agricultural	ADJ
biotropia-182	2	41	university	university	NOUN
biotropia-182	2	42	,	,	PUNCT
biotropia-182	2	43	bogor-16144	bogor-16144	PROPN
biotropia-182	2	44	,	,	PUNCT
biotropia-182	2	45	indonesia	indonesia	PROPN
biotropia-182	2	46	:	:	PUNCT
biotropia-182	2	47	'	'	PUNCT
biotropia-182	2	48	'	'	PUNCT
biotropia-182	2	49	department	department	NOUN
biotropia-182	2	50	of	of	ADP
biotropia-182	2	51	crop	crop	NOUN
biotropia-182	2	52	science	science	NOUN
biotropia-182	2	53	.	.	PUNCT
biotropia-182	3	1	scottish	scottish	ADJ
biotropia-182	3	2	agricultural	agricultural	PROPN
biotropia-182	3	3	college	college	PROPN
biotropia-182	3	4	,	,	PUNCT
biotropia-182	3	5	edinburgh	edinburgh	PROPN
biotropia-182	3	6	eh9	eh9	PROPN
biotropia-182	3	7	3jg	3jg	PROPN
biotropia-182	3	8	,	,	PUNCT
biotropia-182	3	9	uk	uk	PROPN
biotropia-182	3	10	.	.	PROPN
biotropia-182	4	1	abstract	abstract	ADJ
biotropia-182	4	2	a	a	DET
biotropia-182	4	3	transcriptional	transcriptional	ADJ
biotropia-182	4	4	fusion	fusion	NOUN
biotropia-182	4	5	for	for	ADP
biotropia-182	4	6	an	an	DET
biotropia-182	4	7	aeromonas	aeromonas	PROPN
biotropia-182	4	8	caviae	caviae	PROPN
biotropia-182	4	9	chitinase	chitinase	NOUN
biotropia-182	4	10	gene	gene	NOUN
biotropia-182	4	11	was	be	AUX
biotropia-182	4	12	constructed	construct	VERB
biotropia-182	4	13	under	under	ADP
biotropia-182	4	14	the	the	DET
biotropia-182	4	15	control	control	NOUN
biotropia-182	4	16	of	of	ADP
biotropia-182	4	17	a	a	DET
biotropia-182	4	18	constitutive	constitutive	ADJ
biotropia-182	4	19	promoter	promoter	NOUN
biotropia-182	4	20	of	of	ADP
biotropia-182	4	21	the	the	DET
biotropia-182	4	22	kanaraycin	kanaraycin	PROPN
biotropia-182	4	23	resistance	resistance	NOUN
biotropia-182	4	24	gene	gene	NOUN
biotropia-182	4	25	(	(	PUNCT
biotropia-182	4	26	pkmr	pkmr	NOUN
biotropia-182	4	27	)	)	PUNCT
biotropia-182	4	28	.	.	PUNCT
biotropia-182	5	1	the	the	DET
biotropia-182	5	2	construct	construct	NOUN
biotropia-182	5	3	was	be	AUX
biotropia-182	5	4	inserted	insert	VERB
biotropia-182	5	5	into	into	ADP
biotropia-182	5	6	a	a	DET
biotropia-182	5	7	medium	medium	ADJ
biotropia-182	5	8	copy	copy	NOUN
biotropia-182	5	9	number	number	NOUN
biotropia-182	5	10	broad	broad	ADJ
biotropia-182	5	11	host	host	NOUN
biotropia-182	5	12	range	range	NOUN
biotropia-182	5	13	plasmid	plasmid	VERB
biotropia-182	5	14	vector	vector	NOUN
biotropia-182	5	15	to	to	PART
biotropia-182	5	16	yield	yield	VERB
biotropia-182	5	17	recombinant	recombinant	ADJ
biotropia-182	5	18	plasmid	plasmid	ADJ
biotropia-182	5	19	pam340	pam340	NOUN
biotropia-182	5	20	,	,	PUNCT
biotropia-182	5	21	which	which	PRON
biotropia-182	5	22	harbored	harbor	VERB
biotropia-182	5	23	transcriptional	transcriptional	ADJ
biotropia-182	5	24	fusion	fusion	NOUN
biotropia-182	5	25	pkmrchi	pkmrchi	NOUN
biotropia-182	5	26	.	.	PUNCT
biotropia-182	6	1	another	another	DET
biotropia-182	6	2	transcriptional	transcriptional	ADJ
biotropia-182	6	3	fusion	fusion	NOUN
biotropia-182	6	4	,	,	PUNCT
biotropia-182	6	5	ptac	ptac	NOUN
biotropia-182	6	6	-	-	PUNCT
biotropia-182	6	7	chi	chi	PROPN
biotropia-182	6	8	,	,	PUNCT
biotropia-182	6	9	in	in	ADP
biotropia-182	6	10	a	a	DET
biotropia-182	6	11	recombinant	recombinant	ADJ
biotropia-182	6	12	plasmid	plasmid	ADJ
biotropia-182	6	13	pam630	pam630	NOUN
biotropia-182	6	14	,	,	PUNCT
biotropia-182	6	15	was	be	AUX
biotropia-182	6	16	conducted	conduct	VERB
biotropia-182	6	17	as	as	ADP
biotropia-182	6	18	comparison	comparison	NOUN
biotropia-182	6	19	.	.	PUNCT
biotropia-182	7	1	triparental	triparental	ADJ
biotropia-182	7	2	mating	mating	NOUN
biotropia-182	7	3	of	of	ADP
biotropia-182	7	4	e.	e.	PROPN
biotropia-182	7	5	coli	coli	NOUN
biotropia-182	7	6	carrying	carry	VERB
biotropia-182	7	7	the	the	DET
biotropia-182	7	8	recombinant	recombinant	ADJ
biotropia-182	7	9	plasmids	plasmid	NOUN
biotropia-182	7	10	with	with	ADP
biotropia-182	7	11	pseudomotws	pseudomotws	NOUN
biotropia-182	7	12	fluorescens	fluorescen	NOUN
biotropia-182	7	13	5100	5100	NUM
biotropia-182	7	14	,	,	PUNCT
biotropia-182	7	15	a	a	DET
biotropia-182	7	16	phyllosphere	phyllosphere	NOUN
biotropia-182	7	17	bacterium	bacterium	NOUN
biotropia-182	7	18	,	,	PUNCT
biotropia-182	7	19	was	be	AUX
biotropia-182	7	20	performed	perform	VERB
biotropia-182	7	21	.	.	PUNCT
biotropia-182	8	1	pseudomonas	pseudomona	NOUN
biotropia-182	8	2	fluorescens	fluorescen	NOUN
biotropia-182	8	3	5100	5100	NUM
biotropia-182	8	4	exconjugants	exconjugant	NOUN
biotropia-182	8	5	were	be	AUX
biotropia-182	8	6	examined	examine	VERB
biotropia-182	8	7	for	for	ADP
biotropia-182	8	8	constitutive	constitutive	ADJ
biotropia-182	8	9	expression	expression	NOUN
biotropia-182	8	10	of	of	ADP
biotropia-182	8	11	chitinase	chitinase	NOUN
biotropia-182	8	12	employing	employ	VERB
biotropia-182	8	13	a	a	DET
biotropia-182	8	14	spectrophotometric	spectrophotometric	ADJ
biotropia-182	8	15	assay	assay	NOUN
biotropia-182	8	16	;	;	PUNCT
biotropia-182	8	17	they	they	PRON
biotropia-182	8	18	showed	show	VERB
biotropia-182	8	19	stronger	strong	ADJ
biotropia-182	8	20	chitin	chitin	NOUN
biotropia-182	8	21	degradation	degradation	NOUN
biotropia-182	8	22	activity	activity	NOUN
biotropia-182	8	23	than	than	ADP
biotropia-182	8	24	escherichia	escherichia	NOUN
biotropia-182	8	25	coli	coli	NOUN
biotropia-182	8	26	transformants	transformant	NOUN
biotropia-182	8	27	.	.	PUNCT
biotropia-182	9	1	using	use	VERB
biotropia-182	9	2	a	a	DET
biotropia-182	9	3	fungal	fungal	ADJ
biotropia-182	9	4	antagonism	antagonism	NOUN
biotropia-182	9	5	plate	plate	NOUN
biotropia-182	9	6	assay	assay	VERB
biotropia-182	9	7	,	,	PUNCT
biotropia-182	9	8	this	this	DET
biotropia-182	9	9	chitinolytic	chitinolytic	ADJ
biotropia-182	9	10	p.	p.	NOUN
biotropia-182	9	11	fluorescens	fluorescen	NOUN
biotropia-182	9	12	,	,	PUNCT
biotropia-182	9	13	however	however	ADV
biotropia-182	9	14	,	,	PUNCT
biotropia-182	9	15	could	could	AUX
biotropia-182	9	16	not	not	PART
biotropia-182	9	17	inhibit	inhibit	AUX
biotropia-182	9	18	selected	select	VERB
biotropia-182	9	19	phytopathogenic	phytopathogenic	ADJ
biotropia-182	9	20	fungi	fungus	NOUN
biotropia-182	9	21	.	.	PUNCT
biotropia-182	10	1	keywords	keyword	NOUN
biotropia-182	10	2	:	:	PUNCT
biotropia-182	10	3	aeromonas	aeromonas	PROPN
biotropia-182	10	4	caviae/	caviae/	NUM
biotropia-182	10	5	chitinase	chitinase	PROPN
biotropia-182	10	6	gene	gene	NOUN
biotropia-182	10	7	/	/	SYM
biotropia-182	10	8	transcriptional	transcriptional	ADJ
biotropia-182	10	9	fusion	fusion	NOUN
biotropia-182	10	10	/	/	SYM
biotropia-182	10	11	pkm'v	pkm'v	PROPN
biotropia-182	10	12	vtac	vtac	NOUN
biotropia-182	10	13	-	-	PUNCT
biotropia-182	10	14	chilpseudomonas	chilpseudomona	NOUN
biotropia-182	10	15	fluorescens	fluorescen	NOUN
biotropia-182	10	16	introduction	introduction	NOUN
biotropia-182	10	17	chitinase	chitinase	NOUN
biotropia-182	10	18	is	be	AUX
biotropia-182	10	19	an	an	DET
biotropia-182	10	20	enzyme	enzyme	NOUN
biotropia-182	10	21	that	that	PRON
biotropia-182	10	22	catalyzes	catalyze	VERB
biotropia-182	10	23	the	the	DET
biotropia-182	10	24	hydrolysis	hydrolysis	NOUN
biotropia-182	10	25	of	of	ADP
biotropia-182	10	26	chitin	chitin	NOUN
biotropia-182	10	27	to	to	ADP
biotropia-182	10	28	oligomers	oligomer	NOUN
biotropia-182	10	29	.	.	PUNCT
biotropia-182	11	1	chitin	chitin	NOUN
biotropia-182	11	2	is	be	AUX
biotropia-182	11	3	an	an	DET
biotropia-182	11	4	insoluble	insoluble	ADJ
biotropia-182	11	5	linear	linear	NOUN
biotropia-182	11	6	p-1	p-1	NOUN
biotropia-182	11	7	,	,	PUNCT
biotropia-182	11	8	4	4	NUM
biotropia-182	11	9	-	-	PUNCT
biotropia-182	11	10	linked	link	VERB
biotropia-182	11	11	unbranched	unbranche	VERB
biotropia-182	11	12	polymer	polymer	NOUN
biotropia-182	11	13	of	of	ADP
biotropia-182	11	14	7v	7v	NOUN
biotropia-182	11	15	-	-	PUNCT
biotropia-182	11	16	acetylgluco	acetylgluco	NOUN
biotropia-182	11	17	-	-	PUNCT
biotropia-182	11	18	samine	samine	NOUN
biotropia-182	11	19	,	,	PUNCT
biotropia-182	11	20	and	and	CCONJ
biotropia-182	11	21	is	be	AUX
biotropia-182	11	22	a	a	DET
biotropia-182	11	23	major	major	ADJ
biotropia-182	11	24	constituent	constituent	NOUN
biotropia-182	11	25	of	of	ADP
biotropia-182	11	26	fungal	fungal	ADJ
biotropia-182	11	27	cell	cell	NOUN
biotropia-182	11	28	wall	wall	NOUN
biotropia-182	11	29	.	.	PUNCT
biotropia-182	12	1	chitinase	chitinase	NOUN
biotropia-182	12	2	is	be	AUX
biotropia-182	12	3	found	find	VERB
biotropia-182	12	4	widely	widely	ADV
biotropia-182	12	5	distributed	distribute	VERB
biotropia-182	12	6	amongst	amongst	ADP
biotropia-182	12	7	many	many	ADJ
biotropia-182	12	8	organisms	organism	NOUN
biotropia-182	12	9	,	,	PUNCT
biotropia-182	12	10	but	but	CCONJ
biotropia-182	12	11	its	its	PRON
biotropia-182	12	12	physiological	physiological	ADJ
biotropia-182	12	13	role	role	NOUN
biotropia-182	12	14	is	be	AUX
biotropia-182	12	15	different	different	ADJ
biotropia-182	12	16	among	among	ADP
biotropia-182	12	17	them	they	PRON
biotropia-182	12	18	.	.	PUNCT
biotropia-182	13	1	in	in	ADP
biotropia-182	13	2	chitinase	chitinase	NOUN
biotropia-182	13	3	-	-	PUNCT
biotropia-182	13	4	producing	produce	VERB
biotropia-182	13	5	plants	plant	NOUN
biotropia-182	13	6	,	,	PUNCT
biotropia-182	13	7	this	this	DET
biotropia-182	13	8	enzyme	enzyme	NOUN
biotropia-182	13	9	is	be	AUX
biotropia-182	13	10	used	use	VERB
biotropia-182	13	11	for	for	ADP
biotropia-182	13	12	self	self	NOUN
biotropia-182	13	13	-	-	PUNCT
biotropia-182	13	14	defense	defense	NOUN
biotropia-182	13	15	against	against	ADP
biotropia-182	13	16	plant	plant	NOUN
biotropia-182	13	17	pathogens	pathogen	NOUN
biotropia-182	13	18	and	and	CCONJ
biotropia-182	13	19	pests	pest	NOUN
biotropia-182	13	20	,	,	PUNCT
biotropia-182	13	21	i.e.	i.e.	X
biotropia-182	13	22	to	to	PART
biotropia-182	13	23	hydrolyse	hydrolyse	VERB
biotropia-182	13	24	chitin	chitin	NOUN
biotropia-182	13	25	in	in	ADP
biotropia-182	13	26	the	the	DET
biotropia-182	13	27	cell	cell	NOUN
biotropia-182	13	28	wall	wall	NOUN
biotropia-182	13	29	of	of	ADP
biotropia-182	13	30	certain	certain	ADJ
biotropia-182	13	31	fungal	fungal	ADJ
biotropia-182	13	32	pathogens	pathogen	NOUN
biotropia-182	13	33	and	and	CCONJ
biotropia-182	13	34	cuticle	cuticle	NOUN
biotropia-182	13	35	of	of	ADP
biotropia-182	13	36	pests	pest	NOUN
biotropia-182	13	37	(	(	PUNCT
biotropia-182	13	38	boiler	boiler	NOUN
biotropia-182	13	39	et	et	PROPN
biotropia-182	13	40	al	al	PROPN
biotropia-182	13	41	.	.	PROPN
biotropia-182	13	42	1983	1983	NUM
biotropia-182	13	43	;	;	PUNCT
biotropia-182	13	44	hedrick	hedrick	PROPN
biotropia-182	13	45	et	et	PROPN
biotropia-182	13	46	a\.	a\.	PROPN
biotropia-182	13	47	1988	1988	NUM
biotropia-182	13	48	;	;	PUNCT
biotropia-182	13	49	oppenheim	oppenheim	PROPN
biotropia-182	13	50	and	and	CCONJ
biotropia-182	13	51	chet	chet	PROPN
biotropia-182	13	52	1992	1992	NUM
biotropia-182	13	53	)	)	PUNCT
biotropia-182	13	54	.	.	PUNCT
biotropia-182	14	1	it	it	PRON
biotropia-182	14	2	has	have	AUX
biotropia-182	14	3	been	be	AUX
biotropia-182	14	4	suggested	suggest	VERB
biotropia-182	14	5	that	that	SCONJ
biotropia-182	14	6	chitinase	chitinase	NOUN
biotropia-182	14	7	production	production	NOUN
biotropia-182	14	8	in	in	ADP
biotropia-182	14	9	plants	plant	NOUN
biotropia-182	14	10	is	be	AUX
biotropia-182	14	11	induced	induce	VERB
biotropia-182	14	12	by	by	ADP
biotropia-182	14	13	microbial	microbial	ADJ
biotropia-182	14	14	infections	infection	NOUN
biotropia-182	14	15	or	or	CCONJ
biotropia-182	14	16	other	other	ADJ
biotropia-182	14	17	injuries	injury	NOUN
biotropia-182	14	18	(	(	PUNCT
biotropia-182	14	19	hedrick	hedrick	PROPN
biotropia-182	14	20	et	et	PROPN
biotropia-182	14	21	al	al	PROPN
biotropia-182	14	22	.	.	PROPN
biotropia-182	14	23	1988	1988	NUM
biotropia-182	14	24	)	)	PUNCT
biotropia-182	14	25	.	.	PUNCT
biotropia-182	15	1	in	in	ADP
biotropia-182	15	2	chitinase	chitinase	NOUN
biotropia-182	15	3	-	-	PUNCT
biotropia-182	15	4	producing	produce	VERB
biotropia-182	15	5	bacteria	bacteria	NOUN
biotropia-182	15	6	,	,	PUNCT
biotropia-182	15	7	extracellular	extracellular	ADJ
biotropia-182	15	8	chitinases	chitinase	NOUN
biotropia-182	15	9	are	be	AUX
biotropia-182	15	10	produced	produce	VERB
biotropia-182	15	11	to	to	PART
biotropia-182	15	12	digest	digest	VERB
biotropia-182	15	13	chitin	chitin	NOUN
biotropia-182	15	14	and	and	CCONJ
biotropia-182	15	15	utilize	utilize	VERB
biotropia-182	15	16	*	*	PUNCT
biotropia-182	15	17	author	author	NOUN
biotropia-182	15	18	to	to	ADP
biotropia-182	15	19	whom	whom	PRON
biotropia-182	15	20	all	all	DET
biotropia-182	15	21	correspondence	correspondence	NOUN
biotropia-182	15	22	and	and	CCONJ
biotropia-182	15	23	reprint	reprint	NOUN
biotropia-182	15	24	requests	request	NOUN
biotropia-182	15	25	should	should	AUX
biotropia-182	15	26	be	be	AUX
biotropia-182	15	27	addressed	address	VERB
biotropia-182	15	28	:	:	PUNCT
biotropia-182	16	1	tel	tel	ADP
biotropia-182	16	2	:	:	PUNCT
biotropia-182	16	3	+62	+62	X
biotropia-182	16	4	(	(	PUNCT
biotropia-182	16	5	251	251	NUM
biotropia-182	16	6	)	)	PUNCT
biotropia-182	16	7	625965	625965	NUM
biotropia-182	16	8	;	;	PUNCT
biotropia-182	16	9	fax:+62	fax:+62	PROPN
biotropia-182	16	10	(	(	PUNCT
biotropia-182	16	11	251	251	NUM
biotropia-182	16	12	)	)	PUNCT
biotropia-182	16	13	315107	315107	NUM
biotropia-182	16	14	;	;	PUNCT
biotropia-182	17	1	e	e	X
biotropia-182	17	2	-	-	NOUN
biotropia-182	17	3	mail	mail	NOUN
biotropia-182	17	4	:	:	PUNCT
biotropia-182	17	5	asuwanto@indo.net.id	asuwanto@indo.net.id	NOUN
biotropia-182	17	6	+	+	CCONJ
biotropia-182	17	7	present	present	ADJ
biotropia-182	17	8	address	address	NOUN
biotropia-182	17	9	:	:	PUNCT
biotropia-182	17	10	department	department	NOUN
biotropia-182	17	11	of	of	ADP
biotropia-182	17	12	pharmacy	pharmacy	NOUN
biotropia-182	17	13	,	,	PUNCT
biotropia-182	17	14	faculty	faculty	NOUN
biotropia-182	17	15	of	of	ADP
biotropia-182	17	16	mathematics	mathematic	NOUN
biotropia-182	17	17	and	and	CCONJ
biotropia-182	17	18	natural	natural	ADJ
biotropia-182	17	19	sciences	science	NOUN
biotropia-182	17	20	,	,	PUNCT
biotropia-182	17	21	university	university	NOUN
biotropia-182	17	22	oflndonesia	oflndonesia	NOUN
biotropia-182	17	23	.	.	PUNCT
biotropia-182	18	1	biotropia	biotropia	PROPN
biotropia-182	18	2	no	no	PROPN
biotropia-182	18	3	.	.	PUNCT
biotropia-182	19	1	20,2003	20,2003	NUM
biotropia-182	19	2	it	it	PRON
biotropia-182	19	3	primarily	primarily	ADV
biotropia-182	19	4	as	as	ADP
biotropia-182	19	5	a	a	DET
biotropia-182	19	6	carbon	carbon	NOUN
biotropia-182	19	7	and	and	CCONJ
biotropia-182	19	8	energy	energy	NOUN
biotropia-182	19	9	source	source	NOUN
biotropia-182	19	10	(	(	PUNCT
biotropia-182	19	11	gooday	gooday	NOUN
biotropia-182	19	12	1990	1990	NUM
biotropia-182	19	13	)	)	PUNCT
biotropia-182	19	14	.	.	PUNCT
biotropia-182	20	1	the	the	DET
biotropia-182	20	2	exploration	exploration	NOUN
biotropia-182	20	3	and	and	CCONJ
biotropia-182	20	4	utilization	utilization	NOUN
biotropia-182	20	5	of	of	ADP
biotropia-182	20	6	bacterial	bacterial	ADJ
biotropia-182	20	7	chitinase	chitinase	NOUN
biotropia-182	20	8	in	in	ADP
biotropia-182	20	9	plant	plant	NOUN
biotropia-182	20	10	protection	protection	NOUN
biotropia-182	20	11	is	be	AUX
biotropia-182	20	12	centred	centre	VERB
biotropia-182	20	13	around	around	ADP
biotropia-182	20	14	its	its	PRON
biotropia-182	20	15	use	use	NOUN
biotropia-182	20	16	to	to	PART
biotropia-182	20	17	protect	protect	VERB
biotropia-182	20	18	against	against	ADP
biotropia-182	20	19	pathogenic	pathogenic	ADJ
biotropia-182	20	20	fungi	fungus	NOUN
biotropia-182	20	21	as	as	ADP
biotropia-182	20	22	a	a	DET
biotropia-182	20	23	biocontrol	biocontrol	NOUN
biotropia-182	20	24	mechanism	mechanism	NOUN
biotropia-182	20	25	.	.	PUNCT
biotropia-182	21	1	this	this	DET
biotropia-182	21	2	potential	potential	NOUN
biotropia-182	21	3	has	have	AUX
biotropia-182	21	4	been	be	AUX
biotropia-182	21	5	a	a	DET
biotropia-182	21	6	major	major	ADJ
biotropia-182	21	7	concern	concern	NOUN
biotropia-182	21	8	in	in	ADP
biotropia-182	21	9	constructing	construct	VERB
biotropia-182	21	10	biocontrol	biocontrol	NOUN
biotropia-182	21	11	agents	agent	NOUN
biotropia-182	21	12	against	against	ADP
biotropia-182	21	13	fungi	fungi	PROPN
biotropia-182	21	14	.	.	PUNCT
biotropia-182	22	1	several	several	ADJ
biotropia-182	22	2	studies	study	NOUN
biotropia-182	22	3	regarding	regard	VERB
biotropia-182	22	4	this	this	PRON
biotropia-182	22	5	have	have	AUX
biotropia-182	22	6	been	be	AUX
biotropia-182	22	7	reported	report	VERB
biotropia-182	22	8	(	(	PUNCT
biotropia-182	22	9	inbar	inbar	NOUN
biotropia-182	22	10	and	and	CCONJ
biotropia-182	22	11	chet	chet	PROPN
biotropia-182	22	12	1991	1991	NUM
biotropia-182	22	13	;	;	PUNCT
biotropia-182	22	14	koby	koby	PROPN
biotropia-182	22	15	et	et	PROPN
biotropia-182	22	16	al	al	PROPN
biotropia-182	22	17	.	.	PROPN
biotropia-182	22	18	1994	1994	NUM
biotropia-182	22	19	)	)	PUNCT
biotropia-182	22	20	.	.	PUNCT
biotropia-182	23	1	we	we	PRON
biotropia-182	23	2	have	have	AUX
biotropia-182	23	3	cloned	clone	VERB
biotropia-182	23	4	and	and	CCONJ
biotropia-182	23	5	sequenced	sequence	VERB
biotropia-182	23	6	a	a	DET
biotropia-182	23	7	heterogenous	heterogenous	ADJ
biotropia-182	23	8	chitinase	chitinase	NOUN
biotropia-182	23	9	gene	gene	NOUN
biotropia-182	23	10	(	(	PUNCT
biotropia-182	23	11	chi	chi	PROPN
biotropia-182	23	12	)	)	PUNCT
biotropia-182	23	13	from	from	ADP
biotropia-182	23	14	a	a	DET
biotropia-182	23	15	soilborne	soilborne	ADJ
biotropia-182	23	16	aeromonas	aeromonas	PROPN
biotropia-182	23	17	caviae	caviae	NOUN
biotropia-182	23	18	isolated	isolate	VERB
biotropia-182	23	19	from	from	ADP
biotropia-182	23	20	a	a	DET
biotropia-182	23	21	blackpepper	blackpepper	NOUN
biotropia-182	23	22	plantation	plantation	NOUN
biotropia-182	23	23	in	in	ADP
biotropia-182	23	24	bangka	bangka	PROPN
biotropia-182	23	25	island	island	PROPN
biotropia-182	23	26	,	,	PUNCT
biotropia-182	23	27	indonesia	indonesia	PROPN
biotropia-182	23	28	in	in	ADP
biotropia-182	23	29	our	our	PRON
biotropia-182	23	30	previous	previous	ADJ
biotropia-182	23	31	study	study	NOUN
biotropia-182	23	32	(	(	PUNCT
biotropia-182	23	33	malik	malik	PROPN
biotropia-182	23	34	et	et	PROPN
biotropia-182	23	35	al	al	PROPN
biotropia-182	23	36	.	.	PROPN
biotropia-182	24	1	in	in	ADP
biotropia-182	24	2	press	press	NOUN
biotropia-182	24	3	)	)	PUNCT
biotropia-182	24	4	.	.	PUNCT
biotropia-182	25	1	this	this	DET
biotropia-182	25	2	gene	gene	NOUN
biotropia-182	25	3	has	have	VERB
biotropia-182	25	4	97	97	NUM
biotropia-182	25	5	%	%	NOUN
biotropia-182	25	6	identity	identity	NOUN
biotropia-182	25	7	with	with	ADP
biotropia-182	25	8	the	the	DET
biotropia-182	25	9	chitinase	chitinase	NOUN
biotropia-182	25	10	gene	gene	NOUN
biotropia-182	25	11	chia	chia	NOUN
biotropia-182	25	12	from	from	ADP
biotropia-182	25	13	a.	a.	NOUN
biotropia-182	25	14	caviae	caviae	NOUN
biotropia-182	25	15	(	(	PUNCT
biotropia-182	25	16	uo9139	uo9139	ADJ
biotropia-182	25	17	)	)	PUNCT
biotropia-182	25	18	(	(	PUNCT
biotropia-182	25	19	sitrit	sitrit	NOUN
biotropia-182	25	20	et	et	PROPN
biotropia-182	25	21	al	al	PROPN
biotropia-182	25	22	.	.	PROPN
biotropia-182	25	23	1995	1995	NUM
biotropia-182	25	24	)	)	PUNCT
biotropia-182	25	25	.	.	PUNCT
biotropia-182	26	1	in	in	ADP
biotropia-182	26	2	this	this	DET
biotropia-182	26	3	present	present	ADJ
biotropia-182	26	4	study	study	NOUN
biotropia-182	26	5	,	,	PUNCT
biotropia-182	26	6	we	we	PRON
biotropia-182	26	7	intended	intend	VERB
biotropia-182	26	8	to	to	PART
biotropia-182	26	9	utilize	utilize	VERB
biotropia-182	26	10	this	this	DET
biotropia-182	26	11	gene	gene	NOUN
biotropia-182	26	12	under	under	ADP
biotropia-182	26	13	control	control	NOUN
biotropia-182	26	14	of	of	ADP
biotropia-182	26	15	a	a	DET
biotropia-182	26	16	constitutive	constitutive	ADJ
biotropia-182	26	17	promoter	promoter	NOUN
biotropia-182	26	18	,	,	PUNCT
biotropia-182	26	19	i.e.	i.e.	X
biotropia-182	26	20	the	the	DET
biotropia-182	26	21	kanamycin	kanamycin	PROPN
biotropia-182	26	22	resistance	resistance	NOUN
biotropia-182	26	23	gene	gene	NOUN
biotropia-182	26	24	from	from	ADP
biotropia-182	26	25	tn903	tn903	PROPN
biotropia-182	26	26	,	,	PUNCT
biotropia-182	26	27	in	in	ADP
biotropia-182	26	28	a	a	DET
biotropia-182	26	29	suitable	suitable	ADJ
biotropia-182	26	30	vector	vector	NOUN
biotropia-182	26	31	for	for	ADP
biotropia-182	26	32	expression	expression	NOUN
biotropia-182	26	33	in	in	ADP
biotropia-182	26	34	a	a	DET
biotropia-182	26	35	phyllosphere	phyllosphere	NOUN
biotropia-182	26	36	bacterium	bacterium	NOUN
biotropia-182	26	37	,	,	PUNCT
biotropia-182	26	38	as	as	ADP
biotropia-182	26	39	a	a	DET
biotropia-182	26	40	means	means	NOUN
biotropia-182	26	41	of	of	ADP
biotropia-182	26	42	producing	produce	VERB
biotropia-182	26	43	a	a	DET
biotropia-182	26	44	chitinolytic	chitinolytic	ADJ
biotropia-182	26	45	biocontrol	biocontrol	NOUN
biotropia-182	26	46	agent	agent	NOUN
biotropia-182	26	47	.	.	PUNCT
biotropia-182	27	1	we	we	PRON
biotropia-182	27	2	cloned	clone	VERB
biotropia-182	27	3	this	this	DET
biotropia-182	27	4	chi	chi	ADJ
biotropia-182	27	5	gene	gene	NOUN
biotropia-182	27	6	into	into	ADP
biotropia-182	27	7	a	a	DET
biotropia-182	27	8	relatively	relatively	ADV
biotropia-182	27	9	small	small	ADJ
biotropia-182	27	10	size	size	NOUN
biotropia-182	27	11	and	and	CCONJ
biotropia-182	27	12	medium	medium	NOUN
biotropia-182	27	13	copy	copy	NOUN
biotropia-182	27	14	number	number	NOUN
biotropia-182	27	15	broad	broad	ADJ
biotropia-182	27	16	host	host	NOUN
biotropia-182	27	17	range	range	NOUN
biotropia-182	27	18	plasmid	plasmid	ADJ
biotropia-182	27	19	vector	vector	NOUN
biotropia-182	27	20	,	,	PUNCT
biotropia-182	27	21	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	27	22	(	(	PUNCT
biotropia-182	27	23	kovach	kovach	NOUN
biotropia-182	27	24	et	et	PROPN
biotropia-182	27	25	al	al	PROPN
biotropia-182	27	26	.	.	PROPN
biotropia-182	27	27	1994	1994	NUM
biotropia-182	27	28	)	)	PUNCT
biotropia-182	27	29	,	,	PUNCT
biotropia-182	27	30	rather	rather	ADV
biotropia-182	27	31	than	than	ADP
biotropia-182	27	32	into	into	ADP
biotropia-182	27	33	a	a	DET
biotropia-182	27	34	high	high	ADJ
biotropia-182	27	35	copy	copy	NOUN
biotropia-182	27	36	number	number	NOUN
biotropia-182	27	37	plasmid	plasmid	NOUN
biotropia-182	27	38	as	as	SCONJ
biotropia-182	27	39	reported	report	VERB
biotropia-182	27	40	by	by	ADP
biotropia-182	27	41	koby	koby	PROPN
biotropia-182	27	42	et	et	PROPN
biotropia-182	27	43	al	al	PROPN
biotropia-182	27	44	.	.	PROPN
biotropia-182	27	45	(	(	PUNCT
biotropia-182	27	46	1994	1994	NUM
biotropia-182	27	47	)	)	PUNCT
biotropia-182	27	48	.	.	PUNCT
biotropia-182	28	1	this	this	DET
biotropia-182	28	2	strategy	strategy	NOUN
biotropia-182	28	3	was	be	AUX
biotropia-182	28	4	employed	employ	VERB
biotropia-182	28	5	to	to	PART
biotropia-182	28	6	avoid	avoid	VERB
biotropia-182	28	7	accumulation	accumulation	NOUN
biotropia-182	28	8	of	of	ADP
biotropia-182	28	9	defective	defective	ADJ
biotropia-182	28	10	escherichia	escherichia	PROPN
biotropia-182	28	11	coll	coll	PROPN
biotropia-182	28	12	mutants	mutant	NOUN
biotropia-182	28	13	during	during	ADP
biotropia-182	28	14	high	high	ADJ
biotropia-182	28	15	expression	expression	NOUN
biotropia-182	28	16	of	of	ADP
biotropia-182	28	17	chitinase	chitinase	PROPN
biotropia-182	28	18	(	(	PUNCT
biotropia-182	28	19	koby	koby	PROPN
biotropia-182	28	20	et	et	PROPN
biotropia-182	28	21	al	al	PROPN
biotropia-182	28	22	.	.	PROPN
biotropia-182	28	23	1994	1994	NUM
biotropia-182	28	24	)	)	PUNCT
biotropia-182	28	25	.	.	PUNCT
biotropia-182	29	1	therefore	therefore	ADV
biotropia-182	29	2	,	,	PUNCT
biotropia-182	29	3	the	the	DET
biotropia-182	29	4	aim	aim	NOUN
biotropia-182	29	5	of	of	ADP
biotropia-182	29	6	this	this	DET
biotropia-182	29	7	work	work	NOUN
biotropia-182	29	8	was	be	AUX
biotropia-182	29	9	to	to	PART
biotropia-182	29	10	construct	construct	VERB
biotropia-182	29	11	a	a	DET
biotropia-182	29	12	chitinolytic	chitinolytic	ADJ
biotropia-182	29	13	p.	p.	NOUN
biotropia-182	29	14	fluorescens	fluorescen	NOUN
biotropia-182	29	15	biocontrol	biocontrol	NOUN
biotropia-182	29	16	strain	strain	VERB
biotropia-182	29	17	through	through	ADP
biotropia-182	29	18	the	the	DET
biotropia-182	29	19	introduction	introduction	NOUN
biotropia-182	29	20	of	of	ADP
biotropia-182	29	21	a	a	DET
biotropia-182	29	22	chitinase	chitinase	NOUN
biotropia-182	29	23	gene	gene	NOUN
biotropia-182	29	24	under	under	ADP
biotropia-182	29	25	the	the	DET
biotropia-182	29	26	control	control	NOUN
biotropia-182	29	27	of	of	ADP
biotropia-182	29	28	the	the	DET
biotropia-182	29	29	kmr	kmr	PROPN
biotropia-182	29	30	promoter	promoter	NOUN
biotropia-182	29	31	as	as	ADP
biotropia-182	29	32	a	a	DET
biotropia-182	29	33	transcriptional	transcriptional	ADJ
biotropia-182	29	34	fusion	fusion	NOUN
biotropia-182	29	35	(	(	PUNCT
biotropia-182	29	36	pkmr	pkmr	NOUN
biotropia-182	29	37	-	-	PUNCT
biotropia-182	29	38	c	c	NOUN
biotropia-182	29	39	/	/	SYM
biotropia-182	29	40	j/	j/	VERB
biotropia-182	29	41	)	)	PUNCT
biotropia-182	29	42	.	.	PUNCT
biotropia-182	30	1	materials	material	NOUN
biotropia-182	30	2	and	and	CCONJ
biotropia-182	30	3	methods	method	NOUN
biotropia-182	30	4	bacterial	bacterial	ADJ
biotropia-182	30	5	strains	strain	NOUN
biotropia-182	30	6	,	,	PUNCT
biotropia-182	30	7	plasmids	plasmid	NOUN
biotropia-182	30	8	,	,	PUNCT
biotropia-182	30	9	and	and	CCONJ
biotropia-182	30	10	growth	growth	NOUN
biotropia-182	30	11	conditions	condition	NOUN
biotropia-182	30	12	bacterial	bacterial	ADJ
biotropia-182	30	13	strains	strain	NOUN
biotropia-182	30	14	and	and	CCONJ
biotropia-182	30	15	plasmids	plasmid	NOUN
biotropia-182	30	16	used	use	VERB
biotropia-182	30	17	or	or	CCONJ
biotropia-182	30	18	constructed	construct	VERB
biotropia-182	30	19	during	during	ADP
biotropia-182	30	20	this	this	DET
biotropia-182	30	21	study	study	NOUN
biotropia-182	30	22	are	be	AUX
biotropia-182	30	23	described	describe	VERB
biotropia-182	30	24	in	in	ADP
biotropia-182	30	25	table	table	NOUN
biotropia-182	30	26	1	1	NUM
biotropia-182	30	27	.	.	PUNCT
biotropia-182	30	28	e.	e.	PROPN
biotropia-182	30	29	coli	coli	PROPN
biotropia-182	30	30	strains	strain	NOUN
biotropia-182	30	31	which	which	PRON
biotropia-182	30	32	were	be	AUX
biotropia-182	30	33	used	use	VERB
biotropia-182	30	34	as	as	ADP
biotropia-182	30	35	hosts	host	NOUN
biotropia-182	30	36	for	for	ADP
biotropia-182	30	37	cloning	clone	VERB
biotropia-182	30	38	experiments	experiment	NOUN
biotropia-182	30	39	throughout	throughout	ADP
biotropia-182	30	40	this	this	DET
biotropia-182	30	41	study	study	NOUN
biotropia-182	30	42	,	,	PUNCT
biotropia-182	30	43	and	and	CCONJ
biotropia-182	30	44	their	their	PRON
biotropia-182	30	45	derivatives	derivative	NOUN
biotropia-182	30	46	also	also	ADV
biotropia-182	30	47	,	,	PUNCT
biotropia-182	30	48	were	be	AUX
biotropia-182	30	49	routinely	routinely	ADV
biotropia-182	30	50	grown	grow	VERB
biotropia-182	30	51	in	in	ADP
biotropia-182	30	52	luria	luria	PROPN
biotropia-182	30	53	bertani	bertani	NOUN
biotropia-182	30	54	(	(	PUNCT
biotropia-182	30	55	lb	lb	NOUN
biotropia-182	30	56	)	)	PUNCT
biotropia-182	30	57	medium	medium	NOUN
biotropia-182	30	58	(	(	PUNCT
biotropia-182	30	59	broth	broth	NOUN
biotropia-182	30	60	or	or	CCONJ
biotropia-182	30	61	solidified	solidify	VERB
biotropia-182	30	62	with	with	ADP
biotropia-182	30	63	1.5	1.5	NUM
biotropia-182	30	64	%	%	NOUN
biotropia-182	30	65	agar	agar	NOUN
biotropia-182	30	66	)	)	PUNCT
biotropia-182	30	67	at	at	ADP
biotropia-182	30	68	37	37	NUM
biotropia-182	30	69	°	°	ADP
biotropia-182	30	70	c	c	NOUN
biotropia-182	30	71	for	for	ADP
biotropia-182	30	72	18	18	NUM
biotropia-182	30	73	hours	hour	NOUN
biotropia-182	30	74	.	.	PUNCT
biotropia-182	31	1	ampicillin	ampicillin	NOUN
biotropia-182	31	2	(	(	PUNCT
biotropia-182	31	3	100	100	NUM
biotropia-182	31	4	ng	ng	PROPN
biotropia-182	31	5	/	/	SYM
biotropia-182	31	6	ml	ml	PROPN
biotropia-182	31	7	)	)	PUNCT
biotropia-182	31	8	,	,	PUNCT
biotropia-182	31	9	kanamycin	kanamycin	PROPN
biotropia-182	31	10	(	(	PUNCT
biotropia-182	31	11	25	25	NUM
biotropia-182	31	12	μg	μg	NOUN
biotropia-182	31	13	/	/	SYM
biotropia-182	31	14	ml	ml	NOUN
biotropia-182	31	15	)	)	PUNCT
biotropia-182	31	16	,	,	PUNCT
biotropia-182	31	17	trimethoprim	trimethoprim	PROPN
biotropia-182	31	18	(	(	PUNCT
biotropia-182	31	19	100	100	NUM
biotropia-182	31	20	μg	μg	NOUN
biotropia-182	31	21	/	/	SYM
biotropia-182	31	22	ml	ml	NOUN
biotropia-182	31	23	)	)	PUNCT
biotropia-182	31	24	and	and	CCONJ
biotropia-182	31	25	gentamycin	gentamycin	VERB
biotropia-182	31	26	(	(	PUNCT
biotropia-182	31	27	10	10	NUM
biotropia-182	31	28	μg	μg	NOUN
biotropia-182	31	29	/	/	SYM
biotropia-182	31	30	ml	ml	NOUN
biotropia-182	31	31	)	)	PUNCT
biotropia-182	31	32	were	be	AUX
biotropia-182	31	33	added	add	VERB
biotropia-182	31	34	to	to	ADP
biotropia-182	31	35	the	the	DET
biotropia-182	31	36	growth	growth	NOUN
biotropia-182	31	37	media	medium	NOUN
biotropia-182	31	38	when	when	SCONJ
biotropia-182	31	39	needed	need	VERB
biotropia-182	31	40	.	.	PUNCT
biotropia-182	32	1	pseudomonas	pseudomona	NOUN
biotropia-182	32	2	fluorescens	fluorescen	NOUN
biotropia-182	32	3	strains	strain	NOUN
biotropia-182	32	4	were	be	AUX
biotropia-182	32	5	grown	grow	VERB
biotropia-182	32	6	routinely	routinely	ADV
biotropia-182	32	7	in	in	ADP
biotropia-182	32	8	king	king	NOUN
biotropia-182	32	9	's	's	PART
biotropia-182	32	10	b	b	PROPN
biotropia-182	32	11	(	(	PUNCT
biotropia-182	32	12	kb	kb	PROPN
biotropia-182	32	13	)	)	PUNCT
biotropia-182	32	14	10	10	NUM
biotropia-182	32	15	%	%	NOUN
biotropia-182	32	16	agar	agar	NOUN
biotropia-182	32	17	(	(	PUNCT
biotropia-182	32	18	king	king	NOUN
biotropia-182	32	19	et	et	PROPN
biotropia-182	32	20	al	al	PROPN
biotropia-182	32	21	.	.	PROPN
biotropia-182	32	22	1954	1954	NUM
biotropia-182	32	23	)	)	PUNCT
biotropia-182	32	24	at	at	ADP
biotropia-182	32	25	25	25	NUM
biotropia-182	32	26	°	°	ADP
biotropia-182	32	27	c	c	NOUN
biotropia-182	32	28	for	for	ADP
biotropia-182	32	29	18	18	NUM
biotropia-182	32	30	-	-	SYM
biotropia-182	32	31	20	20	NUM
biotropia-182	32	32	hours	hour	NOUN
biotropia-182	32	33	.	.	PUNCT
biotropia-182	33	1	spontaneous	spontaneous	ADJ
biotropia-182	33	2	rifampicin	rifampicin	VERB
biotropia-182	33	3	resistant	resistant	ADJ
biotropia-182	33	4	mutants	mutant	NOUN
biotropia-182	33	5	(	(	PUNCT
biotropia-182	33	6	rii	rii	PROPN
biotropia-182	33	7	)	)	PUNCT
biotropia-182	33	8	of	of	ADP
biotropia-182	33	9	p.	p.	PROPN
biotropia-182	33	10	fluorescens	fluorescen	NOUN
biotropia-182	33	11	,	,	PUNCT
biotropia-182	33	12	which	which	PRON
biotropia-182	33	13	was	be	AUX
biotropia-182	33	14	done	do	VERB
biotropia-182	33	15	according	accord	VERB
biotropia-182	33	16	to	to	ADP
biotropia-182	33	17	the	the	DET
biotropia-182	33	18	method	method	NOUN
biotropia-182	33	19	described	describe	VERB
biotropia-182	33	20	by	by	ADP
biotropia-182	33	21	eisenstadt	eisenstadt	PROPN
biotropia-182	33	22	et	et	PROPN
biotropia-182	33	23	al	al	PROPN
biotropia-182	33	24	.	.	PROPN
biotropia-182	33	25	(	(	PUNCT
biotropia-182	33	26	1994	1994	NUM
biotropia-182	33	27	)	)	PUNCT
biotropia-182	33	28	,	,	PUNCT
biotropia-182	33	29	were	be	AUX
biotropia-182	33	30	isolated	isolate	VERB
biotropia-182	33	31	by	by	ADP
biotropia-182	33	32	plating	plate	VERB
biotropia-182	33	33	a	a	DET
biotropia-182	33	34	25ul	25ul	NOUN
biotropia-182	33	35	aliquot	aliquot	NOUN
biotropia-182	33	36	of	of	ADP
biotropia-182	33	37	a	a	DET
biotropia-182	33	38	10	10	NUM
biotropia-182	33	39	-	-	ADJ
biotropia-182	33	40	fold	fold	ADJ
biotropia-182	33	41	concentration	concentration	NOUN
biotropia-182	33	42	of	of	ADP
biotropia-182	33	43	a	a	DET
biotropia-182	33	44	stationary	stationary	ADJ
biotropia-182	33	45	phase	phase	NOUN
biotropia-182	33	46	culture	culture	NOUN
biotropia-182	33	47	onto	onto	ADP
biotropia-182	33	48	plates	plate	NOUN
biotropia-182	33	49	containing	contain	VERB
biotropia-182	33	50	a	a	DET
biotropia-182	33	51	gradient	gradient	ADJ
biotropia-182	33	52	concentration	concentration	NOUN
biotropia-182	33	53	from	from	ADP
biotropia-182	33	54	10	10	NUM
biotropia-182	33	55	to	to	PART
biotropia-182	33	56	100	100	NUM
biotropia-182	33	57	ug	ug	ADP
biotropia-182	33	58	/	/	SYM
biotropia-182	33	59	ml	ml	NOUN
biotropia-182	33	60	of	of	ADP
biotropia-182	33	61	rifampicin	rifampicin	VERB
biotropia-182	33	62	.	.	PUNCT
biotropia-182	34	1	dna	dna	PROPN
biotropia-182	34	2	manipulation	manipulation	NOUN
biotropia-182	34	3	plasmid	plasmid	NOUN
biotropia-182	34	4	dnas	dna	NOUN
biotropia-182	34	5	were	be	AUX
biotropia-182	34	6	isolated	isolate	VERB
biotropia-182	34	7	either	either	CCONJ
biotropia-182	34	8	by	by	ADP
biotropia-182	34	9	alkaline	alkaline	ADJ
biotropia-182	34	10	lysis	lysis	NOUN
biotropia-182	34	11	(	(	PUNCT
biotropia-182	34	12	sambrook	sambrook	PROPN
biotropia-182	34	13	et	et	PROPN
biotropia-182	34	14	al	al	PROPN
biotropia-182	34	15	.	.	PROPN
biotropia-182	34	16	1989	1989	NUM
biotropia-182	34	17	)	)	PUNCT
biotropia-182	34	18	,	,	PUNCT
biotropia-182	34	19	or	or	CCONJ
biotropia-182	34	20	hv	hv	PROPN
biotropia-182	34	21	dna	dna	PROPN
biotropia-182	34	22	nurification	nurification	PROPN
biotropia-182	34	23	kits	kit	NOUN
biotropia-182	34	24	(	(	PUNCT
biotropia-182	34	25	promeea	promeea	VERB
biotropia-182	34	26	wis	wis	PROPN
biotropia-182	34	27	..	..	PUNCT
biotropia-182	34	28	or	or	CCONJ
biotropia-182	34	29	biorad	biorad	NOUN
biotropia-182	34	30	,	,	PUNCT
biotropia-182	34	31	richmond	richmond	PROPN
biotropia-182	34	32	,	,	PUNCT
biotropia-182	34	33	calif	calif	PROPN
biotropia-182	34	34	.	.	PUNCT
biotropia-182	34	35	)	)	PUNCT
biotropia-182	34	36	.	.	PUNCT
biotropia-182	35	1	restriction	restriction	NOUN
biotropia-182	35	2	heterologous	heterologous	ADJ
biotropia-182	35	3	expression	expression	NOUN
biotropia-182	35	4	of	of	ADP
biotropia-182	35	5	a	a	DET
biotropia-182	35	6	chitinase	chitinase	NOUN
biotropia-182	35	7	gene	gene	NOUN
biotropia-182	35	8	from	from	ADP
biotropia-182	35	9	aeromonas	aeromonas	PROPN
biotropia-182	35	10	caviae	caviae	PROPN
biotropia-182	35	11	amarila	amarila	PROPN
biotropia-182	35	12	malik	malik	PROPN
biotropia-182	35	13	el	el	PROPN
biotropia-182	35	14	al	al	PROPN
biotropia-182	35	15	table	table	PROPN
biotropia-182	35	16	1	1	NUM
biotropia-182	35	17	.	.	PUNCT
biotropia-182	36	1	bacterial	bacterial	ADJ
biotropia-182	36	2	strains	strain	NOUN
biotropia-182	36	3	and	and	CCONJ
biotropia-182	36	4	piasmids	piasmid	NOUN
biotropia-182	36	5	bacterial	bacterial	ADJ
biotropia-182	36	6	strains	strain	NOUN
biotropia-182	36	7	and	and	CCONJ
biotropia-182	36	8	plasm	plasm	NOUN
biotropia-182	36	9	relevant	relevant	ADJ
biotropia-182	36	10	characteristics	characteristic	NOUN
biotropia-182	36	11	reference	reference	NOUN
biotropia-182	36	12	or	or	CCONJ
biotropia-182	36	13	source	source	NOUN
biotropia-182	36	14	e.	e.	PROPN
biotropia-182	36	15	coli	coli	PROPN
biotropia-182	36	16	sambrook	sambrook	PROPN
biotropia-182	36	17	et	et	PROPN
biotropia-182	36	18	dh5a	dh5a	NOUN
biotropia-182	36	19	f	f	PROPN
biotropia-182	36	20	"	"	PUNCT
biotropia-182	36	21	,	,	PUNCT
biotropia-182	36	22	for	for	ADP
biotropia-182	36	23	a	a	DET
biotropia-182	36	24	-	-	PUNCT
biotropia-182	36	25	complementation	complementation	NOUN
biotropia-182	36	26	,	,	PUNCT
biotropia-182	36	27	general	general	ADJ
biotropia-182	36	28	host	host	NOUN
biotropia-182	36	29	for	for	ADP
biotropia-182	36	30	cloning	clone	VERB
biotropia-182	36	31	al	al	PROPN
biotropia-182	36	32	.	.	PROPN
biotropia-182	36	33	1989	1989	NUM
biotropia-182	36	34	top10	top10	PROPN
biotropia-182	36	35	f	f	X
biotropia-182	36	36	"	"	PUNCT
biotropia-182	36	37	,	,	PUNCT
biotropia-182	36	38	for	for	ADP
biotropia-182	36	39	a	a	DET
biotropia-182	36	40	-complementation	-complementation	NOUN
biotropia-182	36	41	,	,	PUNCT
biotropia-182	36	42	general	general	ADJ
biotropia-182	36	43	host	host	NOUN
biotropia-182	36	44	for	for	ADP
biotropia-182	36	45	cloning	clone	VERB
biotropia-182	36	46	invitrogen	invitrogen	PROPN
biotropia-182	36	47	,	,	PUNCT
biotropia-182	36	48	inc	inc	PROPN
biotropia-182	36	49	.	.	PROPN
biotropia-182	37	1	(	(	PUNCT
biotropia-182	37	2	carlsbad	carlsbad	PROPN
biotropia-182	37	3	,	,	PUNCT
biotropia-182	37	4	calif	calif	PROPN
biotropia-182	37	5	.	.	PUNCT
biotropia-182	37	6	)	)	PUNCT
biotropia-182	38	1	hb101(prk2013	hb101(prk2013	PROPN
biotropia-182	38	2	)	)	PUNCT
biotropia-182	38	3	mod	mod	ADJ
biotropia-182	38	4	"	"	PUNCT
biotropia-182	38	5	res	re	NOUN
biotropia-182	38	6	"	"	PUNCT
biotropia-182	38	7	,	,	PUNCT
biotropia-182	38	8	general	general	ADJ
biotropia-182	38	9	helper	helper	NOUN
biotropia-182	38	10	strain	strain	VERB
biotropia-182	38	11	for	for	ADP
biotropia-182	38	12	bacterial	bacterial	ADJ
biotropia-182	38	13	conjugation	conjugation	NOUN
biotropia-182	38	14	dittae	dittae	PROPN
biotropia-182	38	15	/	/	SYM
biotropia-182	39	1	fl/1980	fl/1980	PROPN
biotropia-182	39	2	top10(pam340	top10(pam340	NUM
biotropia-182	39	3	)	)	PUNCT
biotropia-182	39	4	top10	top10	NOUN
biotropia-182	39	5	carries	carry	VERB
biotropia-182	39	6	fusion	fusion	NOUN
biotropia-182	39	7	transcription	transcription	NOUN
biotropia-182	39	8	pkmr	pkmr	NOUN
biotropia-182	39	9	-	-	PUNCT
biotropia-182	39	10	c	c	NOUN
biotropia-182	39	11	/	/	SYM
biotropia-182	39	12	i	i	NOUN
biotropia-182	39	13	;	;	PUNCT
biotropia-182	39	14	this	this	DET
biotropia-182	39	15	study	study	NOUN
biotropia-182	39	16	top10(pam630	top10(pam630	NOUN
biotropia-182	39	17	)	)	PUNCT
biotropia-182	39	18	top10	top10	PROPN
biotropia-182	39	19	carries	carry	VERB
biotropia-182	39	20	fusion	fusion	NOUN
biotropia-182	39	21	transcription	transcription	NOUN
biotropia-182	39	22	ptac	ptac	NOUN
biotropia-182	39	23	-	-	PUNCT
biotropia-182	39	24	chi	chi	ADJ
biotropia-182	39	25	this	this	DET
biotropia-182	39	26	study	study	NOUN
biotropia-182	39	27	p.jluorescens	p.jluorescen	VERB
biotropia-182	39	28	5100	5100	NUM
biotropia-182	39	29	phyllosphere	phyllosphere	NOUN
biotropia-182	39	30	biosurfactant	biosurfactant	NOUN
biotropia-182	39	31	*	*	PROPN
biotropia-182	39	32	strain	strain	PROPN
biotropia-182	39	33	,	,	PUNCT
biotropia-182	39	34	wildtype	wildtype	NOUN
biotropia-182	39	35	,	,	PUNCT
biotropia-182	39	36	derived	derive	VERB
biotropia-182	39	37	from	from	ADP
biotropia-182	39	38	campbell	campbell	PROPN
biotropia-182	39	39	et	et	PROPN
biotropia-182	39	40	al	al	PROPN
biotropia-182	39	41	brassica	brassica	PROPN
biotropia-182	39	42	oleracea	oleracea	PROPN
biotropia-182	39	43	var	var	PROPN
biotropia-182	39	44	italica	italica	PROPN
biotropia-182	39	45	1995	1995	NUM
biotropia-182	39	46	5100	5100	NUM
biotropia-182	39	47	rif	rif	PROPN
biotropia-182	39	48	spontaneous	spontaneous	ADJ
biotropia-182	39	49	rifampicin	rifampicin	VERB
biotropia-182	39	50	resistance	resistance	NOUN
biotropia-182	39	51	mutant	mutant	NOUN
biotropia-182	39	52	pfsloo	pfsloo	VERB
biotropia-182	39	53	this	this	DET
biotropia-182	39	54	study	study	NOUN
biotropia-182	39	55	5100(pam340	5100(pam340	NUM
biotropia-182	39	56	)	)	PUNCT
biotropia-182	39	57	pf	pf	NOUN
biotropia-182	39	58	5100	5100	NUM
biotropia-182	39	59	(	(	PUNCT
biotropia-182	39	60	rif	rif	PROPN
biotropia-182	39	61	)	)	PUNCT
biotropia-182	39	62	carries	carry	VERB
biotropia-182	39	63	fusion	fusion	NOUN
biotropia-182	39	64	transcription	transcription	NOUN
biotropia-182	39	65	pkmr	pkmr	NOUN
biotropia-182	39	66	-	-	PUNCT
biotropia-182	39	67	c	c	NOUN
biotropia-182	39	68	/	/	SYM
biotropia-182	39	69	i	i	NOUN
biotropia-182	39	70	;	;	PUNCT
biotropia-182	39	71	this	this	DET
biotropia-182	39	72	study	study	NOUN
biotropia-182	39	73	5100(pam630	5100(pam630	NUM
biotropia-182	39	74	)	)	PUNCT
biotropia-182	39	75	pf	pf	ADP
biotropia-182	39	76	5100	5100	NUM
biotropia-182	39	77	(	(	PUNCT
biotropia-182	39	78	rif	rif	PROPN
biotropia-182	39	79	)	)	PUNCT
biotropia-182	39	80	carries	carry	VERB
biotropia-182	39	81	fusion	fusion	NOUN
biotropia-182	39	82	transcription	transcription	NOUN
biotropia-182	39	83	ptac	ptac	NOUN
biotropia-182	39	84	-	-	PUNCT
biotropia-182	39	85	chi	chi	NOUN
biotropia-182	39	86	this	this	DET
biotropia-182	39	87	study	study	NOUN
biotropia-182	39	88	aeromonas	aeromonas	PROPN
biotropia-182	39	89	caviae	caviae	PROPN
biotropia-182	39	90	ws7b	ws7b	PROPN
biotropia-182	39	91	chitinolytic	chitinolytic	PROPN
biotropia-182	39	92	,	,	PUNCT
biotropia-182	39	93	wildtype	wildtype	PROPN
biotropia-182	39	94	malik	malik	PROPN
biotropia-182	39	95	etal	etal	PROPN
biotropia-182	39	96	in	in	ADP
biotropia-182	39	97	press	press	NOUN
biotropia-182	39	98	plasmids	plasmid	NOUN
biotropia-182	39	99	puc19	puc19	NOUN
biotropia-182	39	100	lacz	lacz	NOUN
biotropia-182	39	101	.	.	PUNCT
biotropia-182	40	1	apr	apr	NOUN
biotropia-182	40	2	cloning	cloning	NOUN
biotropia-182	40	3	vector	vector	NOUN
biotropia-182	40	4	sambrook	sambrook	NOUN
biotropia-182	40	5	et	et	PROPN
biotropia-182	40	6	al	al	PROPN
biotropia-182	40	7	.	.	PROPN
biotropia-182	40	8	1989	1989	NUM
biotropia-182	41	1	pas385	pas385	PROPN
biotropia-182	41	2	mcs	mcs	PROPN
biotropia-182	41	3	from	from	ADP
biotropia-182	41	4	psl301(£cori-.eo	psl301(£cori-.eo	PROPN
biotropia-182	41	5	/	/	SYM
biotropia-182	41	6	i	i	PROPN
biotropia-182	41	7	)	)	PUNCT
biotropia-182	41	8	cloned	clone	VERB
biotropia-182	41	9	into	into	ADP
biotropia-182	41	10	puc19	puc19	NOUN
biotropia-182	41	11	suwanto	suwanto	NOUN
biotropia-182	41	12	&	&	CCONJ
biotropia-182	41	13	kaplan	kaplan	PROPN
biotropia-182	41	14	(	(	PUNCT
biotropia-182	41	15	smal	smal	NOUN
biotropia-182	41	16	-	-	PUNCT
biotropia-182	41	17	ecorl	ecorl	NOUN
biotropia-182	41	18	)	)	PUNCT
biotropia-182	41	19	,	,	PUNCT
biotropia-182	41	20	apr	apr	PROPN
biotropia-182	41	21	1992	1992	NUM
biotropia-182	41	22	pas396	pas396	NOUN
biotropia-182	41	23	source	source	NOUN
biotropia-182	41	24	of	of	ADP
biotropia-182	41	25	trimethoprim	trimethoprim	PROPN
biotropia-182	41	26	resistance	resistance	NOUN
biotropia-182	41	27	gene	gene	NOUN
biotropia-182	41	28	(	(	PUNCT
biotropia-182	41	29	tpr	tpr	NOUN
biotropia-182	41	30	)	)	PUNCT
biotropia-182	41	31	in	in	ADP
biotropia-182	41	32	pas385	pas385	PROPN
biotropia-182	41	33	suwanto	suwanto	PROPN
biotropia-182	41	34	&	&	CCONJ
biotropia-182	41	35	kaplan	kaplan	PROPN
biotropia-182	41	36	1992	1992	NUM
biotropia-182	41	37	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	41	38	broad	broad	ADJ
biotropia-182	41	39	-	-	PUNCT
biotropia-182	41	40	host	host	NOUN
biotropia-182	41	41	range	range	NOUN
biotropia-182	41	42	,	,	PUNCT
biotropia-182	41	43	medium	medium	ADJ
biotropia-182	41	44	copy	copy	NOUN
biotropia-182	41	45	number	number	NOUN
biotropia-182	41	46	,	,	PUNCT
biotropia-182	41	47	kmr	kmr	PROPN
biotropia-182	41	48	kovache	kovache	PROPN
biotropia-182	41	49	/	/	SYM
biotropia-182	41	50	a/.	a/.	ADJ
biotropia-182	41	51	1994	1994	NUM
biotropia-182	41	52	puc4k	puc4k	PROPN
biotropia-182	41	53	pkmr	pkmr	PROPN
biotropia-182	41	54	source	source	PROPN
biotropia-182	41	55	,	,	PUNCT
biotropia-182	41	56	kmr	kmr	PROPN
biotropia-182	41	57	gene	gene	NOUN
biotropia-182	41	58	from	from	ADP
biotropia-182	41	59	1n903	1n903	NUM
biotropia-182	41	60	oknetal	oknetal	ADJ
biotropia-182	41	61	1981	1981	NUM
biotropia-182	41	62	p34s	p34s	PROPN
biotropia-182	41	63	-	-	PUNCT
biotropia-182	41	64	gm	gm	PROPN
biotropia-182	41	65	source	source	NOUN
biotropia-182	41	66	of	of	ADP
biotropia-182	41	67	gentamycin	gentamycin	VERB
biotropia-182	41	68	resistance	resistance	NOUN
biotropia-182	41	69	gene	gene	NOUN
biotropia-182	41	70	(	(	PUNCT
biotropia-182	41	71	gmr	gmr	PROPN
biotropia-182	41	72	)	)	PUNCT
biotropia-182	41	73	dennis	dennis	PROPN
biotropia-182	41	74	&	&	CCONJ
biotropia-182	41	75	zylstra	zylstra	PROPN
biotropia-182	41	76	1998	1998	NUM
biotropia-182	42	1	pws506	pws506	PROPN
biotropia-182	42	2	pas385	pas385	PROPN
biotropia-182	42	3	carrying	carry	VERB
biotropia-182	42	4	2.9	2.9	NUM
biotropia-182	42	5	kb	kb	PROPN
biotropia-182	42	6	xho\	xho\	PROPN
biotropia-182	42	7	+	+	CCONJ
biotropia-182	42	8	ffmdlu	ffmdlu	PROPN
biotropia-182	42	9	chitinase	chitinase	NOUN
biotropia-182	42	10	gene	gene	NOUN
biotropia-182	42	11	malik	malik	PROPN
biotropia-182	42	12	et	et	PROPN
biotropia-182	42	13	al	al	PROPN
biotropia-182	42	14	in	in	ADP
biotropia-182	42	15	fragment	fragment	NOUN
biotropia-182	42	16	from	from	ADP
biotropia-182	42	17	a.	a.	NOUN
biotropia-182	42	18	caviae	caviae	PROPN
biotropia-182	42	19	ws7b	ws7b	PROPN
biotropia-182	42	20	press	press	NOUN
biotropia-182	42	21	pam201	pam201	NOUN
biotropia-182	42	22	c	c	PROPN
biotropia-182	42	23	/	/	SYM
biotropia-182	42	24	i/-tpr	i/-tpr	ADJ
biotropia-182	42	25	dna	dna	NOUN
biotropia-182	42	26	fragment	fragment	NOUN
biotropia-182	42	27	as	as	ADP
biotropia-182	42	28	sail	sail	NOUN
biotropia-182	42	29	cassette	cassette	NOUN
biotropia-182	42	30	in	in	ADP
biotropia-182	42	31	pas385	pas385	PROPN
biotropia-182	42	32	this	this	DET
biotropia-182	42	33	study	study	NOUN
biotropia-182	42	34	pam330	pam330	PROPN
biotropia-182	42	35	pknf	pknf	NOUN
biotropia-182	42	36	-	-	PUNCT
biotropia-182	42	37	chi	chi	NOUN
biotropia-182	42	38	in	in	ADP
biotropia-182	42	39	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	42	40	,	,	PUNCT
biotropia-182	42	41	tpr	tpr	PROPN
biotropia-182	42	42	this	this	DET
biotropia-182	42	43	study	study	NOUN
biotropia-182	42	44	pam340	pam340	NOUN
biotropia-182	42	45	pkmr	pkmr	PROPN
biotropia-182	42	46	-	-	PUNCT
biotropia-182	42	47	c	c	NOUN
biotropia-182	42	48	/	/	SYM
biotropia-182	42	49	i/	i/	NOUN
biotropia-182	42	50	in	in	ADP
biotropia-182	42	51	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	42	52	,	,	PUNCT
biotropia-182	42	53	tp	tp	PART
biotropia-182	42	54	"	"	PUNCT
biotropia-182	42	55	,	,	PUNCT
biotropia-182	42	56	gmr	gmr	PROPN
biotropia-182	42	57	this	this	DET
biotropia-182	42	58	study	study	NOUN
biotropia-182	42	59	pam630	pam630	PROPN
biotropia-182	42	60	ptac	ptac	NOUN
biotropia-182	42	61	-	-	PUNCT
biotropia-182	42	62	chi	chi	NOUN
biotropia-182	42	63	in	in	ADP
biotropia-182	42	64	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	42	65	,	,	PUNCT
biotropia-182	42	66	gmr	gmr	NOUN
biotropia-182	42	67	this	this	DET
biotropia-182	42	68	study	study	NOUN
biotropia-182	42	69	fragments	fragment	NOUN
biotropia-182	42	70	were	be	AUX
biotropia-182	42	71	purified	purify	VERB
biotropia-182	42	72	from	from	ADP
biotropia-182	42	73	agarose	agarose	NOUN
biotropia-182	42	74	gels	gel	NOUN
biotropia-182	42	75	with	with	ADP
biotropia-182	42	76	the	the	DET
biotropia-182	42	77	gene	gene	NOUN
biotropia-182	42	78	clean	clean	NOUN
biotropia-182	42	79	kit	kit	NOUN
biotropia-182	42	80	(	(	PUNCT
biotropia-182	42	81	biolol	biolol	PROPN
biotropia-182	42	82	inc	inc	PROPN
biotropia-182	42	83	.	.	PROPN
biotropia-182	42	84	,	,	PUNCT
biotropia-182	42	85	la	la	PROPN
biotropia-182	42	86	jolla	jolla	PROPN
biotropia-182	42	87	,	,	PUNCT
biotropia-182	42	88	calif	calif	PROPN
biotropia-182	42	89	.	.	PUNCT
biotropia-182	42	90	)	)	PUNCT
biotropia-182	42	91	.	.	PUNCT
biotropia-182	43	1	dna	dna	PROPN
biotropia-182	43	2	filled	fill	VERB
biotropia-182	43	3	-	-	PUNCT
biotropia-182	43	4	in	in	ADP
biotropia-182	43	5	reaction	reaction	NOUN
biotropia-182	43	6	,	,	PUNCT
biotropia-182	43	7	utilizing	utilize	VERB
biotropia-182	43	8	t4	t4	PROPN
biotropia-182	43	9	dna	dna	PROPN
biotropia-182	43	10	polymerase	polymerase	NOUN
biotropia-182	43	11	(	(	PUNCT
biotropia-182	43	12	boehringer	boehringer	PROPN
biotropia-182	43	13	mannheim	mannheim	PROPN
biotropia-182	43	14	biochemicals	biochemical	NOUN
biotropia-182	43	15	,	,	PUNCT
biotropia-182	43	16	indianapolis	indianapolis	PROPN
biotropia-182	43	17	,	,	PUNCT
biotropia-182	43	18	ind	ind	PROPN
biotropia-182	43	19	.	.	PUNCT
biotropia-182	43	20	)	)	PUNCT
biotropia-182	44	1	,	,	PUNCT
biotropia-182	44	2	dna	dna	PROPN
biotropia-182	44	3	fragment	fragment	NOUN
biotropia-182	44	4	ligations	ligation	NOUN
biotropia-182	44	5	,	,	PUNCT
biotropia-182	44	6	recombinant	recombinant	ADJ
biotropia-182	44	7	dna	dna	NOUN
biotropia-182	44	8	transformation	transformation	NOUN
biotropia-182	44	9	,	,	PUNCT
biotropia-182	44	10	and	and	CCONJ
biotropia-182	44	11	other	other	ADJ
biotropia-182	44	12	accessory	accessory	NOUN
biotropia-182	44	13	techniques	technique	NOUN
biotropia-182	44	14	were	be	AUX
biotropia-182	44	15	carried	carry	VERB
biotropia-182	44	16	out	out	ADP
biotropia-182	44	17	as	as	SCONJ
biotropia-182	44	18	described	describe	VERB
biotropia-182	44	19	by	by	ADP
biotropia-182	44	20	sambrook	sambrook	PROPN
biotropia-182	44	21	et	et	PROPN
biotropia-182	44	22	al	al	PROPN
biotropia-182	44	23	(	(	PUNCT
biotropia-182	44	24	1989	1989	NUM
biotropia-182	44	25	)	)	PUNCT
biotropia-182	44	26	.	.	PUNCT
biotropia-182	45	1	escherichia	escherichia	NOUN
biotropia-182	45	2	coli	coli	NOUN
biotropia-182	45	3	transformants	transformant	NOUN
biotropia-182	45	4	harboring	harbor	VERB
biotropia-182	45	5	the	the	DET
biotropia-182	45	6	chitinase	chitinase	NOUN
biotropia-182	45	7	recombinant	recombinant	NOUN
biotropia-182	45	8	were	be	AUX
biotropia-182	45	9	selected	select	VERB
biotropia-182	45	10	on	on	ADP
biotropia-182	45	11	lb	lb	DET
biotropia-182	45	12	-	-	PUNCT
biotropia-182	45	13	chitin	chitin	NOUN
biotropia-182	45	14	agar	agar	NOUN
biotropia-182	45	15	plates	plate	NOUN
biotropia-182	45	16	,	,	PUNCT
biotropia-182	45	17	supplemented	supplement	VERB
biotropia-182	45	18	with	with	ADP
biotropia-182	45	19	the	the	DET
biotropia-182	45	20	appro	appro	ADJ
biotropia-182	45	21	biotropia	biotropia	ADJ
biotropia-182	45	22	no	no	NOUN
biotropia-182	45	23	.	.	PUNCT
biotropia-182	46	1	20,2003	20,2003	NUM
biotropia-182	46	2	ing	ing	ADJ
biotropia-182	46	3	pkmr	pkmr	NOUN
biotropia-182	46	4	,	,	PUNCT
biotropia-182	46	5	as	as	ADV
biotropia-182	46	6	well	well	ADV
biotropia-182	46	7	as	as	ADP
biotropia-182	46	8	ptac	ptac	NOUN
biotropia-182	46	9	,	,	PUNCT
biotropia-182	46	10	were	be	AUX
biotropia-182	46	11	selected	select	VERB
biotropia-182	46	12	by	by	ADP
biotropia-182	46	13	a	a	DET
biotropia-182	46	14	blue	blue	ADJ
biotropia-182	46	15	-	-	PUNCT
biotropia-182	46	16	white	white	ADJ
biotropia-182	46	17	assay	assay	NOUN
biotropia-182	46	18	on	on	ADP
biotropia-182	46	19	lb	lb	DET
biotropia-182	46	20	agar	agar	NOUN
biotropia-182	46	21	plates	plate	NOUN
biotropia-182	46	22	,	,	PUNCT
biotropia-182	46	23	containing	contain	VERB
biotropia-182	46	24	iptg	iptg	NOUN
biotropia-182	46	25	(	(	PUNCT
biotropia-182	46	26	0.5	0.5	NUM
biotropia-182	46	27	mm	mm	NOUN
biotropia-182	46	28	)	)	PUNCT
biotropia-182	46	29	,	,	PUNCT
biotropia-182	46	30	and	and	CCONJ
biotropia-182	46	31	x	x	X
biotropia-182	46	32	-	-	ADJ
biotropia-182	46	33	gal	gal	ADJ
biotropia-182	46	34	(	(	PUNCT
biotropia-182	46	35	5	5	NUM
biotropia-182	46	36	-	-	PUNCT
biotropia-182	46	37	bromo-4	bromo-4	ADP
biotropia-182	46	38	-	-	PUNCT
biotropia-182	46	39	chloro-3	chloro-3	ADJ
biotropia-182	46	40	-	-	PUNCT
biotropia-182	46	41	indolyl	indolyl	NOUN
biotropia-182	46	42	-	-	PUNCT
biotropia-182	46	43	b	b	NOUN
biotropia-182	46	44	-	-	PUNCT
biotropia-182	46	45	d	d	NOUN
biotropia-182	46	46	-	-	PUNCT
biotropia-182	46	47	thiogalacto	thiogalacto	NOUN
biotropia-182	46	48	-	-	PUNCT
biotropia-182	46	49	pyranoside	pyranoside	NOUN
biotropia-182	46	50	)	)	PUNCT
biotropia-182	46	51	40	40	NUM
biotropia-182	46	52	mg	mg	NUM
biotropia-182	46	53	/	/	SYM
biotropia-182	46	54	ml	ml	NOUN
biotropia-182	46	55	.	.	PUNCT
biotropia-182	47	1	plasmids	plasmid	NOUN
biotropia-182	47	2	were	be	AUX
biotropia-182	47	3	maintained	maintain	VERB
biotropia-182	47	4	by	by	ADP
biotropia-182	47	5	selection	selection	NOUN
biotropia-182	47	6	in	in	ADP
biotropia-182	47	7	the	the	DET
biotropia-182	47	8	presence	presence	NOUN
biotropia-182	47	9	of	of	ADP
biotropia-182	47	10	appropriate	appropriate	ADJ
biotropia-182	47	11	antibiotics	antibiotic	NOUN
biotropia-182	47	12	.	.	PUNCT
biotropia-182	48	1	bacterial	bacterial	ADJ
biotropia-182	48	2	conjugation	conjugation	NOUN
biotropia-182	48	3	recombinant	recombinant	ADJ
biotropia-182	48	4	plasmids	plasmid	NOUN
biotropia-182	48	5	pkmr	pkmr	NOUN
biotropia-182	48	6	-	-	PUNCT
biotropia-182	48	7	c/	c/	NOUN
biotropia-182	48	8	»	»	PUNCT
biotropia-182	48	9	and	and	CCONJ
biotropia-182	48	10	ptac	ptac	NOUN
biotropia-182	48	11	-	-	PUNCT
biotropia-182	48	12	chi	chi	PROPN
biotropia-182	48	13	were	be	AUX
biotropia-182	48	14	introduced	introduce	VERB
biotropia-182	48	15	into	into	ADP
biotropia-182	48	16	p.	p.	PROPN
biotropia-182	48	17	fluorescens	fluorescen	NOUN
biotropia-182	48	18	5100	5100	NUM
biotropia-182	48	19	(	(	PUNCT
biotropia-182	48	20	campbell	campbell	PROPN
biotropia-182	48	21	et	et	PROPN
biotropia-182	48	22	al	al	PROPN
biotropia-182	48	23	.	.	PROPN
biotropia-182	48	24	1995	1995	NUM
biotropia-182	48	25	)	)	PUNCT
biotropia-182	48	26	by	by	ADP
biotropia-182	48	27	bacterial	bacterial	ADJ
biotropia-182	48	28	conjugation	conjugation	NOUN
biotropia-182	48	29	as	as	SCONJ
biotropia-182	48	30	described	describe	VERB
biotropia-182	48	31	by	by	ADP
biotropia-182	48	32	ditta	ditta	PROPN
biotropia-182	48	33	et	et	PROPN
biotropia-182	48	34	al	al	PROPN
biotropia-182	48	35	(	(	PUNCT
biotropia-182	48	36	1980	1980	NUM
biotropia-182	48	37	)	)	PUNCT
biotropia-182	48	38	.	.	PUNCT
biotropia-182	49	1	triparental	triparental	ADJ
biotropia-182	49	2	mating	mating	NOUN
biotropia-182	49	3	was	be	AUX
biotropia-182	49	4	performed	perform	VERB
biotropia-182	49	5	,	,	PUNCT
biotropia-182	49	6	employing	employ	VERB
biotropia-182	49	7	e.	e.	PROPN
biotropia-182	49	8	coli	coli	PROPN
biotropia-182	49	9	hb101	hb101	PROPN
biotropia-182	49	10	(	(	PUNCT
biotropia-182	49	11	prk2013	prk2013	PROPN
biotropia-182	49	12	)	)	PUNCT
biotropia-182	49	13	(	(	PUNCT
biotropia-182	49	14	ditta	ditta	PROPN
biotropia-182	49	15	et	et	PROPN
biotropia-182	49	16	al	al	PROPN
biotropia-182	49	17	.	.	PROPN
biotropia-182	49	18	1980	1980	NUM
biotropia-182	49	19	)	)	PUNCT
biotropia-182	49	20	as	as	ADP
biotropia-182	49	21	helper	helper	NOUN
biotropia-182	49	22	,	,	PUNCT
biotropia-182	49	23	e.	e.	PROPN
biotropia-182	49	24	coli	coli	PROPN
biotropia-182	49	25	top10	top10	PROPN
biotropia-182	49	26	(	(	PUNCT
biotropia-182	49	27	pam340	pam340	NOUN
biotropia-182	49	28	)	)	PUNCT
biotropia-182	49	29	,	,	PUNCT
biotropia-182	49	30	as	as	ADV
biotropia-182	49	31	well	well	ADV
biotropia-182	49	32	as	as	ADP
biotropia-182	49	33	e.	e.	PROPN
biotropia-182	49	34	coli	coli	PROPN
biotropia-182	49	35	top10	top10	PROPN
biotropia-182	49	36	(	(	PUNCT
biotropia-182	49	37	pam630	pam630	PROPN
biotropia-182	49	38	)	)	PUNCT
biotropia-182	49	39	,	,	PUNCT
biotropia-182	49	40	as	as	ADP
biotropia-182	49	41	donor	donor	NOUN
biotropia-182	49	42	,	,	PUNCT
biotropia-182	49	43	and	and	CCONJ
biotropia-182	49	44	p.	p.	NOUN
biotropia-182	49	45	fluorescens	fluorescen	NOUN
biotropia-182	49	46	5100	5100	NUM
biotropia-182	49	47	rif	rif	PROPN
biotropia-182	49	48	as	as	ADP
biotropia-182	49	49	recipient	recipient	NOUN
biotropia-182	49	50	.	.	PUNCT
biotropia-182	50	1	the	the	DET
biotropia-182	50	2	cells	cell	NOUN
biotropia-182	50	3	of	of	ADP
biotropia-182	50	4	all	all	DET
biotropia-182	50	5	bacteria	bacteria	NOUN
biotropia-182	50	6	employed	employ	VERB
biotropia-182	50	7	in	in	ADP
biotropia-182	50	8	this	this	DET
biotropia-182	50	9	mating	mating	NOUN
biotropia-182	50	10	were	be	AUX
biotropia-182	50	11	collected	collect	VERB
biotropia-182	50	12	from	from	ADP
biotropia-182	50	13	mid	mid	ADJ
biotropia-182	50	14	-	-	ADJ
biotropia-182	50	15	exponential	exponential	ADJ
biotropia-182	50	16	phase	phase	NOUN
biotropia-182	50	17	cultures	culture	NOUN
biotropia-182	50	18	,	,	PUNCT
biotropia-182	50	19	harvested	harvest	VERB
biotropia-182	50	20	by	by	ADP
biotropia-182	50	21	centrifiiging	centrifiige	VERB
biotropia-182	50	22	recipient	recipient	NOUN
biotropia-182	50	23	,	,	PUNCT
biotropia-182	50	24	donor	donor	NOUN
biotropia-182	50	25	,	,	PUNCT
biotropia-182	50	26	and	and	CCONJ
biotropia-182	50	27	helper	helper	NOUN
biotropia-182	50	28	cultures	culture	NOUN
biotropia-182	50	29	in	in	ADP
biotropia-182	50	30	a	a	DET
biotropia-182	50	31	ratio	ratio	NOUN
biotropia-182	50	32	of	of	ADP
biotropia-182	50	33	8:1:1	8:1:1	NUM
biotropia-182	50	34	,	,	PUNCT
biotropia-182	50	35	washing	wash	VERB
biotropia-182	50	36	the	the	DET
biotropia-182	50	37	pellet	pellet	NOUN
biotropia-182	50	38	twice	twice	ADV
biotropia-182	50	39	in	in	ADP
biotropia-182	50	40	sterile	sterile	ADJ
biotropia-182	50	41	saline	saline	NOUN
biotropia-182	50	42	solution	solution	NOUN
biotropia-182	50	43	,	,	PUNCT
biotropia-182	50	44	and	and	CCONJ
biotropia-182	50	45	resuspending	resuspend	VERB
biotropia-182	50	46	in	in	ADP
biotropia-182	50	47	a	a	DET
biotropia-182	50	48	small	small	ADJ
biotropia-182	50	49	volume	volume	NOUN
biotropia-182	50	50	of	of	ADP
biotropia-182	50	51	lb	lb	DET
biotropia-182	50	52	broth	broth	NOUN
biotropia-182	50	53	(	(	PUNCT
biotropia-182	50	54	30	30	NUM
biotropia-182	50	55	ul	ul	NOUN
biotropia-182	50	56	)	)	PUNCT
biotropia-182	50	57	.	.	PUNCT
biotropia-182	51	1	all	all	DET
biotropia-182	51	2	cell	cell	NOUN
biotropia-182	51	3	suspensions	suspension	NOUN
biotropia-182	51	4	were	be	AUX
biotropia-182	51	5	mixed	mix	VERB
biotropia-182	51	6	,	,	PUNCT
biotropia-182	51	7	spotted	spot	VERB
biotropia-182	51	8	onto	onto	ADP
biotropia-182	51	9	lb	lb	NUM
biotropia-182	51	10	agar	agar	NOUN
biotropia-182	51	11	plates	plate	NOUN
biotropia-182	51	12	without	without	ADP
biotropia-182	51	13	any	any	DET
biotropia-182	51	14	antibiotic	antibiotic	ADJ
biotropia-182	51	15	supplements	supplement	NOUN
biotropia-182	51	16	,	,	PUNCT
biotropia-182	51	17	and	and	CCONJ
biotropia-182	51	18	incubated	incubate	VERB
biotropia-182	51	19	at	at	ADP
biotropia-182	51	20	25	25	NUM
biotropia-182	51	21	°	°	NOUN
biotropia-182	51	22	c	c	NOUN
biotropia-182	51	23	.	.	PUNCT
biotropia-182	52	1	polymerase	polymerase	NOUN
biotropia-182	52	2	chain	chain	NOUN
biotropia-182	52	3	reactions	reaction	NOUN
biotropia-182	52	4	standard	standard	ADJ
biotropia-182	52	5	pcr	pcr	ADJ
biotropia-182	52	6	reactions	reaction	NOUN
biotropia-182	52	7	were	be	AUX
biotropia-182	52	8	performed	perform	VERB
biotropia-182	52	9	to	to	PART
biotropia-182	52	10	verify	verify	VERB
biotropia-182	52	11	the	the	DET
biotropia-182	52	12	presence	presence	NOUN
biotropia-182	52	13	of	of	ADP
biotropia-182	52	14	cloned	cloned	ADJ
biotropia-182	52	15	chi	chi	NOUN
biotropia-182	52	16	in	in	ADP
biotropia-182	52	17	p.	p.	PROPN
biotropia-182	52	18	fluorescens	fluorescens	PROPN
biotropia-182	52	19	exconjugants	exconjugant	NOUN
biotropia-182	52	20	,	,	PUNCT
biotropia-182	52	21	followed	follow	VERB
biotropia-182	52	22	by	by	ADP
biotropia-182	52	23	electrophoretic	electrophoretic	ADJ
biotropia-182	52	24	analysis	analysis	NOUN
biotropia-182	52	25	.	.	PUNCT
biotropia-182	53	1	an	an	DET
biotropia-182	53	2	approximately	approximately	ADV
biotropia-182	53	3	800	800	NUM
biotropia-182	53	4	-	-	PUNCT
biotropia-182	53	5	bp	bp	NOUN
biotropia-182	53	6	fragment	fragment	NOUN
biotropia-182	53	7	of	of	ADP
biotropia-182	53	8	chi	chi	NOUN
biotropia-182	53	9	(	(	PUNCT
biotropia-182	53	10	accession	accession	NOUN
biotropia-182	53	11	number	number	NOUN
biotropia-182	53	12	aj431785	aj431785	PROPN
biotropia-182	53	13	)	)	PUNCT
biotropia-182	53	14	was	be	AUX
biotropia-182	53	15	amplified	amplify	VERB
biotropia-182	53	16	in	in	ADP
biotropia-182	53	17	a	a	DET
biotropia-182	53	18	geneamp	geneamp	ADJ
biotropia-182	53	19	2400	2400	NUM
biotropia-182	53	20	thermal	thermal	ADJ
biotropia-182	53	21	cycler	cycler	NOUN
biotropia-182	53	22	(	(	PUNCT
biotropia-182	53	23	perkin	perkin	PROPN
biotropia-182	53	24	elmer	elmer	PROPN
biotropia-182	53	25	)	)	PUNCT
biotropia-182	53	26	.	.	PUNCT
biotropia-182	54	1	single	single	ADJ
biotropia-182	54	2	colonies	colony	NOUN
biotropia-182	54	3	of	of	ADP
biotropia-182	54	4	exconjugants	exconjugant	NOUN
biotropia-182	54	5	were	be	AUX
biotropia-182	54	6	resus	resus	NOUN
biotropia-182	54	7	-	-	PUNCT
biotropia-182	54	8	pended	pende	VERB
biotropia-182	54	9	into	into	ADP
biotropia-182	54	10	a	a	DET
biotropia-182	54	11	reaction	reaction	NOUN
biotropia-182	54	12	mixture	mixture	NOUN
biotropia-182	54	13	prepared	prepare	VERB
biotropia-182	54	14	with	with	ADP
biotropia-182	54	15	taqdna	taqdna	NOUN
biotropia-182	54	16	polymerase	polymerase	NOUN
biotropia-182	54	17	(	(	PUNCT
biotropia-182	54	18	finnzyme	finnzyme	NOUN
biotropia-182	54	19	)	)	PUNCT
biotropia-182	54	20	.	.	PUNCT
biotropia-182	55	1	the	the	DET
biotropia-182	55	2	thermocycling	thermocycle	VERB
biotropia-182	55	3	program	program	NOUN
biotropia-182	55	4	used	use	VERB
biotropia-182	55	5	in	in	ADP
biotropia-182	55	6	this	this	DET
biotropia-182	55	7	study	study	NOUN
biotropia-182	55	8	consisted	consist	VERB
biotropia-182	55	9	of	of	ADP
biotropia-182	55	10	denaturation	denaturation	NOUN
biotropia-182	55	11	at	at	ADP
biotropia-182	55	12	94	94	NUM
biotropia-182	55	13	°	°	ADP
biotropia-182	55	14	c	c	NOUN
biotropia-182	55	15	for	for	ADP
biotropia-182	55	16	60	60	NUM
biotropia-182	55	17	s	s	NOUN
biotropia-182	55	18	,	,	PUNCT
biotropia-182	55	19	annealing	anneal	VERB
biotropia-182	55	20	at	at	ADP
biotropia-182	55	21	54	54	NUM
biotropia-182	55	22	°	°	NOUN
biotropia-182	55	23	°	°	ADP
biotropia-182	55	24	c	c	NOUN
biotropia-182	55	25	for	for	ADP
biotropia-182	55	26	60	60	NUM
biotropia-182	55	27	s	s	NOUN
biotropia-182	55	28	,	,	PUNCT
biotropia-182	55	29	and	and	CCONJ
biotropia-182	55	30	elongation	elongation	NOUN
biotropia-182	55	31	at	at	ADP
biotropia-182	55	32	72	72	NUM
biotropia-182	55	33	°	°	NOUN
biotropia-182	55	34	°	°	ADP
biotropia-182	55	35	c	c	NOUN
biotropia-182	55	36	for	for	ADP
biotropia-182	55	37	90	90	NUM
biotropia-182	55	38	s	s	NOUN
biotropia-182	55	39	,	,	PUNCT
biotropia-182	55	40	for	for	ADP
biotropia-182	55	41	25	25	NUM
biotropia-182	55	42	cycles	cycle	NOUN
biotropia-182	55	43	,	,	PUNCT
biotropia-182	55	44	as	as	ADP
biotropia-182	55	45	a	a	DET
biotropia-182	55	46	modification	modification	NOUN
biotropia-182	55	47	of	of	ADP
biotropia-182	55	48	the	the	DET
biotropia-182	55	49	method	method	NOUN
biotropia-182	55	50	described	describe	VERB
biotropia-182	55	51	in	in	ADP
biotropia-182	55	52	chernin	chernin	PROPN
biotropia-182	55	53	et	et	PROPN
biotropia-182	55	54	al	al	PROPN
biotropia-182	55	55	.	.	PROPN
biotropia-182	56	1	(	(	PUNCT
biotropia-182	56	2	1997	1997	NUM
biotropia-182	56	3	)	)	PUNCT
biotropia-182	56	4	.	.	PUNCT
biotropia-182	57	1	a	a	DET
biotropia-182	57	2	set	set	NOUN
biotropia-182	57	3	of	of	ADP
biotropia-182	57	4	chi	chi	ADJ
biotropia-182	57	5	internal	internal	ADJ
biotropia-182	57	6	primers	primer	NOUN
biotropia-182	57	7	,	,	PUNCT
biotropia-182	57	8	which	which	PRON
biotropia-182	57	9	were	be	AUX
biotropia-182	57	10	designed	design	VERB
biotropia-182	57	11	based	base	VERB
biotropia-182	57	12	on	on	ADP
biotropia-182	57	13	a.	a.	NOUN
biotropia-182	57	14	caviae	caviae	PROPN
biotropia-182	57	15	chitinase	chitinase	PROPN
biotropia-182	57	16	dna	dna	PROPN
biotropia-182	57	17	sequence	sequence	NOUN
biotropia-182	57	18	(	(	PUNCT
biotropia-182	57	19	accession	accession	NOUN
biotropia-182	57	20	number	number	NOUN
biotropia-182	57	21	aj431785	aj431785	PROPN
biotropia-182	57	22	)	)	PUNCT
biotropia-182	57	23	,	,	PUNCT
biotropia-182	57	24	were	be	AUX
biotropia-182	57	25	used	use	VERB
biotropia-182	57	26	,	,	PUNCT
biotropia-182	57	27	i.e.	i.e.	X
biotropia-182	57	28	forward	forward	ADJ
biotropia-182	57	29	primer	primer	NOUN
biotropia-182	57	30	5	5	NUM
biotropia-182	57	31	'	'	PART
biotropia-182	57	32	g	g	NOUN
biotropia-182	57	33	t	t	PROPN
biotropia-182	57	34	g	g	PROPN
biotropia-182	57	35	a	a	PRON
biotropia-182	57	36	a	a	DET
biotropia-182	57	37	g	g	NOUN
biotropia-182	57	38	a	a	DET
biotropia-182	57	39	a	a	DET
biotropia-182	57	40	c	c	NOUN
biotropia-182	57	41	t	t	NOUN
biotropia-182	57	42	a	a	PRON
biotropia-182	57	43	c	c	NOUN
biotropia-182	57	44	c	c	NOUN
biotropia-182	57	45	a	a	DET
biotropia-182	57	46	g	g	NOUN
biotropia-182	57	47	g	g	PROPN
biotropia-182	57	48	c	c	NOUN
biotropia-182	57	49	-3	-3	PUNCT
biotropia-182	57	50	'	'	PUNCT
biotropia-182	57	51	,	,	PUNCT
biotropia-182	57	52	and	and	CCONJ
biotropia-182	57	53	reverse	reverse	VERB
biotropia-182	57	54	primer	primer	NOUN
biotropia-182	57	55	5	5	NUM
biotropia-182	57	56	'	'	PART
biotropia-182	57	57	g	g	NOUN
biotropia-182	57	58	g	g	PROPN
biotropia-182	57	59	c	c	PROPN
biotropia-182	57	60	a	a	DET
biotropia-182	57	61	g	g	NOUN
biotropia-182	57	62	a	a	DET
biotropia-182	57	63	t	t	NOUN
biotropia-182	57	64	c	c	NOUN
biotropia-182	57	65	a	a	DET
biotropia-182	57	66	g	g	NOUN
biotropia-182	57	67	t	t	PROPN
biotropia-182	57	68	t	t	NOUN
biotropia-182	57	69	g	g	PROPN
biotropia-182	57	70	c	c	PROPN
biotropia-182	57	71	a	a	DET
biotropia-182	57	72	g	g	NOUN
biotropia-182	57	73	c	c	PROPN
biotropia-182	57	74	t	t	PROPN
biotropia-182	57	75	c	c	PROPN
biotropia-182	57	76	g	g	PROPN
biotropia-182	57	77	-3	-3	PUNCT
biotropia-182	57	78	'	'	PUNCT
biotropia-182	57	79	(	(	PUNCT
biotropia-182	57	80	genset	genset	PROPN
biotropia-182	57	81	biotech	biotech	PROPN
biotropia-182	57	82	,	,	PUNCT
biotropia-182	57	83	singapore	singapore	PROPN
biotropia-182	57	84	)	)	PUNCT
biotropia-182	57	85	.	.	PUNCT
biotropia-182	58	1	chitinase	chitinase	NOUN
biotropia-182	58	2	plate	plate	NOUN
biotropia-182	58	3	assay	assay	PROPN
biotropia-182	58	4	and	and	CCONJ
biotropia-182	58	5	semimicro	semimicro	ADJ
biotropia-182	58	6	-	-	ADJ
biotropia-182	58	7	quantitative	quantitative	ADJ
biotropia-182	58	8	assay	assay	VERB
biotropia-182	58	9	an	an	DET
biotropia-182	58	10	assay	assay	NOUN
biotropia-182	58	11	for	for	ADP
biotropia-182	58	12	chitinase	chitinase	NOUN
biotropia-182	58	13	was	be	AUX
biotropia-182	58	14	carried	carry	VERB
biotropia-182	58	15	out	out	ADP
biotropia-182	58	16	on	on	ADP
biotropia-182	58	17	lb	lb	DET
biotropia-182	58	18	agar	agar	NOUN
biotropia-182	58	19	supplemented	supplement	VERB
biotropia-182	58	20	with	with	ADP
biotropia-182	58	21	colloidal	colloidal	NOUN
biotropia-182	58	22	chitin	chitin	NOUN
biotropia-182	58	23	1	1	NUM
biotropia-182	58	24	%	%	NOUN
biotropia-182	58	25	(	(	PUNCT
biotropia-182	58	26	hsu	hsu	PROPN
biotropia-182	58	27	and	and	CCONJ
biotropia-182	58	28	lockwood	lockwood	PROPN
biotropia-182	58	29	1975	1975	NUM
biotropia-182	58	30	)	)	PUNCT
biotropia-182	58	31	for	for	ADP
biotropia-182	58	32	e.	e.	PROPN
biotropia-182	58	33	coli	coli	NOUN
biotropia-182	58	34	derivatives	derivative	NOUN
biotropia-182	58	35	,	,	PUNCT
biotropia-182	58	36	whilst	whilst	SCONJ
biotropia-182	58	37	for	for	ADP
biotropia-182	58	38	p.	p.	NOUN
biotropia-182	58	39	fluorescens	fluorescen	NOUN
biotropia-182	58	40	derivatives	derivative	NOUN
biotropia-182	58	41	this	this	PRON
biotropia-182	58	42	was	be	AUX
biotropia-182	58	43	carried	carry	VERB
biotropia-182	58	44	out	out	ADP
biotropia-182	58	45	on	on	ADP
biotropia-182	58	46	10	10	NUM
biotropia-182	58	47	%	%	NOUN
biotropia-182	58	48	kb	kb	NOUN
biotropia-182	58	49	agar	agar	NOUN
biotropia-182	58	50	supplemented	supplement	VERB
biotropia-182	58	51	with	with	ADP
biotropia-182	58	52	colloidal	colloidal	NOUN
biotropia-182	58	53	chitin	chitin	NOUN
biotropia-182	58	54	at	at	ADP
biotropia-182	58	55	the	the	DET
biotropia-182	58	56	same	same	ADJ
biotropia-182	58	57	amount	amount	NOUN
biotropia-182	58	58	.	.	PUNCT
biotropia-182	59	1	p.	p.	NOUN
biotropia-182	59	2	fluorescens	fluorescens	PROPN
biotropia-182	59	3	exconjugants	exconjugant	NOUN
biotropia-182	59	4	were	be	AUX
biotropia-182	59	5	selected	select	VERB
biotropia-182	59	6	on	on	ADP
biotropia-182	59	7	10	10	NUM
biotropia-182	59	8	%	%	NOUN
biotropia-182	59	9	kb	kb	NOUN
biotropia-182	59	10	agar	agar	NOUN
biotropia-182	59	11	supplemented	supplement	VERB
biotropia-182	59	12	with	with	ADP
biotropia-182	59	13	rifampicin	rifampicin	VERB
biotropia-182	59	14	(	(	PUNCT
biotropia-182	59	15	50	50	NUM
biotropia-182	59	16	ug	ug	NOUN
biotropia-182	59	17	/	/	SYM
biotropia-182	59	18	ml	ml	NOUN
biotropia-182	59	19	)	)	PUNCT
biotropia-182	59	20	and	and	CCONJ
biotropia-182	59	21	gentamycin	gentamycin	VERB
biotropia-182	59	22	after	after	ADP
biotropia-182	59	23	introducing	introduce	VERB
biotropia-182	59	24	transcriptional	transcriptional	ADJ
biotropia-182	59	25	fusion	fusion	NOUN
biotropia-182	59	26	recombinant	recombinant	ADJ
biotropia-182	59	27	plasmids	plasmid	NOUN
biotropia-182	59	28	by	by	ADP
biotropia-182	59	29	bacterial	bacterial	ADJ
biotropia-182	59	30	conjugation	conjugation	NOUN
biotropia-182	59	31	.	.	PUNCT
biotropia-182	60	1	semimicro	semimicro	ADJ
biotropia-182	60	2	-	-	ADJ
biotropia-182	60	3	quantitative	quantitative	ADJ
biotropia-182	60	4	assay	assay	NOUN
biotropia-182	60	5	was	be	AUX
biotropia-182	60	6	performed	perform	VERB
biotropia-182	60	7	as	as	SCONJ
biotropia-182	60	8	described	describe	VERB
biotropia-182	60	9	previously	previously	ADV
biotropia-182	60	10	(	(	PUNCT
biotropia-182	60	11	malik	malik	PROPN
biotropia-182	60	12	et	et	PROPN
biotropia-182	60	13	a/.	a/.	VERB
biotropia-182	60	14	in	in	ADP
biotropia-182	60	15	press	press	NOUN
biotropia-182	60	16	)	)	PUNCT
biotropia-182	60	17	.	.	PUNCT
biotropia-182	61	1	cultures	culture	NOUN
biotropia-182	61	2	of	of	ADP
biotropia-182	61	3	the	the	DET
biotropia-182	61	4	recombinant	recombinant	ADJ
biotropia-182	61	5	bacteria	bacteria	NOUN
biotropia-182	61	6	were	be	AUX
biotropia-182	61	7	collected	collect	VERB
biotropia-182	61	8	after	after	ADP
biotropia-182	61	9	growing	grow	VERB
biotropia-182	61	10	for	for	ADP
biotropia-182	61	11	18	18	NUM
biotropia-182	61	12	-	-	SYM
biotropia-182	61	13	20	20	NUM
biotropia-182	61	14	hours	hour	NOUN
biotropia-182	61	15	both	both	PRON
biotropia-182	61	16	for	for	ADP
biotropia-182	61	17	e.	e.	PROPN
biotropia-182	61	18	coli	coli	PROPN
biotropia-182	61	19	and	and	CCONJ
biotropia-182	61	20	p.	p.	NOUN
biotropia-182	61	21	fluorescens	fluorescen	NOUN
biotropia-182	61	22	,	,	PUNCT
biotropia-182	61	23	whilst	whilst	SCONJ
biotropia-182	61	24	cultures	culture	NOUN
biotropia-182	61	25	of	of	ADP
biotropia-182	61	26	a.	a.	NOUN
biotropia-182	61	27	caviae	caviae	NOUN
biotropia-182	61	28	were	be	AUX
biotropia-182	61	29	heterologous	heterologous	ADJ
biotropia-182	61	30	expression	expression	NOUN
biotropia-182	61	31	of	of	ADP
biotropia-182	61	32	a	a	DET
biotropia-182	61	33	chitinase	chitinase	NOUN
biotropia-182	61	34	gene	gene	NOUN
biotropia-182	61	35	fromaeromonas	fromaeromonas	PROPN
biotropia-182	61	36	caviae	caviae	PROPN
biotropia-182	61	37	amarila	amarila	PROPN
biotropia-182	61	38	malik	malik	PROPN
biotropia-182	61	39	et	et	PROPN
biotropia-182	61	40	al	al	PROPN
biotropia-182	61	41	.	.	PROPN
biotropia-182	62	1	collected	collect	VERB
biotropia-182	62	2	from	from	ADP
biotropia-182	62	3	15	15	NUM
biotropia-182	62	4	hours	hour	NOUN
biotropia-182	62	5	.	.	PUNCT
biotropia-182	63	1	enzyme	enzyme	NOUN
biotropia-182	63	2	activity	activity	NOUN
biotropia-182	63	3	was	be	AUX
biotropia-182	63	4	measured	measure	VERB
biotropia-182	63	5	in	in	ADP
biotropia-182	63	6	both	both	CCONJ
biotropia-182	63	7	extracellular	extracellular	ADJ
biotropia-182	63	8	and	and	CCONJ
biotropia-182	63	9	intracellular	intracellular	ADJ
biotropia-182	63	10	fractions	fraction	NOUN
biotropia-182	63	11	,	,	PUNCT
biotropia-182	63	12	after	after	SCONJ
biotropia-182	63	13	sonicated	sonicate	VERB
biotropia-182	63	14	the	the	DET
biotropia-182	63	15	cell	cell	NOUN
biotropia-182	63	16	.	.	PUNCT
biotropia-182	64	1	the	the	DET
biotropia-182	64	2	protein	protein	NOUN
biotropia-182	64	3	concentration	concentration	NOUN
biotropia-182	64	4	was	be	AUX
biotropia-182	64	5	measured	measure	VERB
biotropia-182	64	6	according	accord	VERB
biotropia-182	64	7	to	to	ADP
biotropia-182	64	8	the	the	DET
biotropia-182	64	9	method	method	NOUN
biotropia-182	64	10	as	as	SCONJ
biotropia-182	64	11	described	describe	VERB
biotropia-182	64	12	by	by	ADP
biotropia-182	64	13	bradford	bradford	NOUN
biotropia-182	64	14	(	(	PUNCT
biotropia-182	64	15	1976	1976	NUM
biotropia-182	64	16	)	)	PUNCT
biotropia-182	64	17	.	.	PUNCT
biotropia-182	65	1	chitinase	chitinase	NOUN
biotropia-182	65	2	activity	activity	NOUN
biotropia-182	65	3	was	be	AUX
biotropia-182	65	4	assayed	assay	VERB
biotropia-182	65	5	by	by	ADP
biotropia-182	65	6	a	a	DET
biotropia-182	65	7	modification	modification	NOUN
biotropia-182	65	8	of	of	ADP
biotropia-182	65	9	methods	method	NOUN
biotropia-182	65	10	as	as	SCONJ
biotropia-182	65	11	described	describe	VERB
biotropia-182	65	12	using	use	VERB
biotropia-182	65	13	colloidal	colloidal	NOUN
biotropia-182	65	14	chitin	chitin	NOUN
biotropia-182	65	15	azure	azure	NOUN
biotropia-182	65	16	as	as	ADP
biotropia-182	65	17	substrate	substrate	NOUN
biotropia-182	65	18	(	(	PUNCT
biotropia-182	65	19	wirth	wirth	PROPN
biotropia-182	65	20	and	and	CCONJ
biotropia-182	65	21	wolf	wolf	PROPN
biotropia-182	65	22	1990	1990	NUM
biotropia-182	65	23	;	;	PUNCT
biotropia-182	65	24	evrall	evrall	NOUN
biotropia-182	65	25	et	et	PROPN
biotropia-182	65	26	al	al	PROPN
biotropia-182	65	27	.	.	PROPN
biotropia-182	65	28	1990	1990	NUM
biotropia-182	65	29	;	;	PUNCT
biotropia-182	65	30	hood	hood	NOUN
biotropia-182	65	31	1990	1990	NUM
biotropia-182	65	32	)	)	PUNCT
biotropia-182	65	33	.	.	PUNCT
biotropia-182	66	1	the	the	DET
biotropia-182	66	2	amount	amount	NOUN
biotropia-182	66	3	of	of	ADP
biotropia-182	66	4	dye	dye	NOUN
biotropia-182	66	5	released	release	VERB
biotropia-182	66	6	from	from	ADP
biotropia-182	66	7	this	this	DET
biotropia-182	66	8	chromogenic	chromogenic	ADJ
biotropia-182	66	9	substrate	substrate	NOUN
biotropia-182	66	10	,	,	PUNCT
biotropia-182	66	11	remazol	remazol	VERB
biotropia-182	66	12	brilliant	brilliant	ADJ
biotropia-182	66	13	blue	blue	NOUN
biotropia-182	66	14	,	,	PUNCT
biotropia-182	66	15	was	be	AUX
biotropia-182	66	16	measured	measure	VERB
biotropia-182	66	17	at	at	ADP
biotropia-182	66	18	590	590	NUM
biotropia-182	66	19	nm	nm	NOUN
biotropia-182	66	20	.	.	PUNCT
biotropia-182	67	1	a	a	DET
biotropia-182	67	2	standard	standard	ADJ
biotropia-182	67	3	curve	curve	NOUN
biotropia-182	67	4	was	be	AUX
biotropia-182	67	5	constructed	construct	VERB
biotropia-182	67	6	using	use	VERB
biotropia-182	67	7	a	a	DET
biotropia-182	67	8	chitinase	chitinase	NOUN
biotropia-182	67	9	standard	standard	NOUN
biotropia-182	67	10	from	from	ADP
biotropia-182	67	11	streptomyces	streptomyce	NOUN
biotropia-182	67	12	griseus	griseus	NOUN
biotropia-182	67	13	(	(	PUNCT
biotropia-182	67	14	sigma	sigma	PROPN
biotropia-182	67	15	chemical	chemical	PROPN
biotropia-182	67	16	,	,	PUNCT
biotropia-182	67	17	st	st	PROPN
biotropia-182	67	18	.	.	PROPN
biotropia-182	67	19	louis	louis	PROPN
biotropia-182	67	20	,	,	PUNCT
biotropia-182	67	21	mo	mo	NOUN
biotropia-182	67	22	)	)	PUNCT
biotropia-182	67	23	against	against	ADP
biotropia-182	67	24	colloidal	colloidal	NOUN
biotropia-182	67	25	chitin	chitin	NOUN
biotropia-182	67	26	azure	azure	NOUN
biotropia-182	67	27	as	as	ADP
biotropia-182	67	28	substrate	substrate	NOUN
biotropia-182	67	29	,	,	PUNCT
biotropia-182	67	30	in	in	ADP
biotropia-182	67	31	citrate	citrate	NOUN
biotropia-182	67	32	-	-	PUNCT
biotropia-182	67	33	phosphate	phosphate	NOUN
biotropia-182	67	34	buffer	buffer	NOUN
biotropia-182	67	35	ph	ph	VERB
biotropia-182	67	36	6.0	6.0	NUM
biotropia-182	67	37	as	as	SCONJ
biotropia-182	67	38	described	describe	VERB
biotropia-182	67	39	by	by	ADP
biotropia-182	67	40	hood	hood	NOUN
biotropia-182	67	41	(	(	PUNCT
biotropia-182	67	42	1990	1990	NUM
biotropia-182	67	43	)	)	PUNCT
biotropia-182	67	44	,	,	PUNCT
biotropia-182	67	45	and	and	CCONJ
biotropia-182	67	46	incubation	incubation	NOUN
biotropia-182	67	47	temperature	temperature	NOUN
biotropia-182	67	48	37	37	NUM
biotropia-182	67	49	°	°	SYM
biotropia-182	67	50	c	c	NOUN
biotropia-182	67	51	for	for	ADP
biotropia-182	67	52	2	2	NUM
biotropia-182	67	53	hours	hour	NOUN
biotropia-182	67	54	.	.	PUNCT
biotropia-182	68	1	the	the	DET
biotropia-182	68	2	enzymatic	enzymatic	ADJ
biotropia-182	68	3	activity	activity	NOUN
biotropia-182	68	4	was	be	AUX
biotropia-182	68	5	expressed	express	VERB
biotropia-182	68	6	as	as	ADP
biotropia-182	68	7	units	unit	NOUN
biotropia-182	68	8	of	of	ADP
biotropia-182	68	9	chitinase	chitinase	NOUN
biotropia-182	68	10	/	/	SYM
biotropia-182	68	11	mg	mg	PROPN
biotropia-182	68	12	of	of	ADP
biotropia-182	68	13	protein	protein	NOUN
biotropia-182	68	14	.	.	PUNCT
biotropia-182	69	1	assay	assay	VERB
biotropia-182	69	2	for	for	ADP
biotropia-182	69	3	antagonism	antagonism	NOUN
biotropia-182	69	4	to	to	PART
biotropia-182	69	5	fungi	fungi	VERB
biotropia-182	69	6	an	an	DET
biotropia-182	69	7	antagonism	antagonism	NOUN
biotropia-182	69	8	assay	assay	NOUN
biotropia-182	69	9	was	be	AUX
biotropia-182	69	10	performed	perform	VERB
biotropia-182	69	11	as	as	SCONJ
biotropia-182	69	12	described	describe	VERB
biotropia-182	69	13	by	by	ADP
biotropia-182	69	14	chernin	chernin	PROPN
biotropia-182	69	15	et	et	PROPN
biotropia-182	69	16	al	al	PROPN
biotropia-182	69	17	.	.	PROPN
biotropia-182	70	1	(	(	PUNCT
biotropia-182	70	2	1995	1995	NUM
biotropia-182	70	3	):	):	PUNCT
biotropia-182	70	4	test	test	NOUN
biotropia-182	70	5	bacteria	bacteria	NOUN
biotropia-182	70	6	were	be	AUX
biotropia-182	70	7	grown	grow	VERB
biotropia-182	70	8	for	for	ADP
biotropia-182	70	9	24	24	NUM
biotropia-182	70	10	and	and	CCONJ
biotropia-182	70	11	48	48	NUM
biotropia-182	70	12	h	h	NOUN
biotropia-182	70	13	for	for	ADP
biotropia-182	70	14	a.	a.	NOUN
biotropia-182	70	15	caviae	caviae	PROPN
biotropia-182	70	16	ws7b	ws7b	PROPN
biotropia-182	70	17	(	(	PUNCT
biotropia-182	70	18	malik	malik	PROPN
biotropia-182	70	19	et	et	PROPN
biotropia-182	70	20	al	al	PROPN
biotropia-182	70	21	.	.	PROPN
biotropia-182	71	1	in	in	ADP
biotropia-182	71	2	press	press	NOUN
biotropia-182	71	3	)	)	PUNCT
biotropia-182	71	4	and	and	CCONJ
biotropia-182	71	5	p.	p.	NOUN
biotropia-182	71	6	fluorescens	fluorescen	NOUN
biotropia-182	71	7	strains	strain	VERB
biotropia-182	71	8	,	,	PUNCT
biotropia-182	71	9	respectively	respectively	ADV
biotropia-182	71	10	,	,	PUNCT
biotropia-182	71	11	in	in	ADP
biotropia-182	71	12	luria	luria	NOUN
biotropia-182	71	13	or	or	CCONJ
biotropia-182	71	14	nutrient	nutrient	ADJ
biotropia-182	71	15	broth	broth	NOUN
biotropia-182	71	16	at	at	ADP
biotropia-182	71	17	30	30	NUM
biotropia-182	72	1	°	°	ADP
biotropia-182	72	2	c	c	NOUN
biotropia-182	72	3	with	with	ADP
biotropia-182	72	4	aeration	aeration	NOUN
biotropia-182	72	5	.	.	PUNCT
biotropia-182	73	1	the	the	DET
biotropia-182	73	2	suspension	suspension	NOUN
biotropia-182	73	3	of	of	ADP
biotropia-182	73	4	cells	cell	NOUN
biotropia-182	73	5	was	be	AUX
biotropia-182	73	6	streaked	streak	VERB
biotropia-182	73	7	in	in	ADP
biotropia-182	73	8	a	a	DET
biotropia-182	73	9	line	line	NOUN
biotropia-182	73	10	at	at	ADP
biotropia-182	73	11	the	the	DET
biotropia-182	73	12	centre	centre	NOUN
biotropia-182	73	13	of	of	ADP
biotropia-182	73	14	a	a	DET
biotropia-182	73	15	pda	pda	NOUN
biotropia-182	73	16	plate	plate	NOUN
biotropia-182	73	17	and	and	CCONJ
biotropia-182	73	18	incubated	incubate	VERB
biotropia-182	73	19	at	at	ADP
biotropia-182	73	20	30	30	NUM
biotropia-182	73	21	°	°	ADP
biotropia-182	73	22	c	c	NOUN
biotropia-182	73	23	for	for	ADP
biotropia-182	73	24	24	24	NUM
biotropia-182	73	25	and	and	CCONJ
biotropia-182	73	26	48	48	NUM
biotropia-182	73	27	h	h	NOUN
biotropia-182	73	28	for	for	ADP
biotropia-182	73	29	ws7b	ws7b	PROPN
biotropia-182	73	30	and	and	CCONJ
biotropia-182	73	31	p.	p.	PROPN
biotropia-182	73	32	fluorescens	fluorescens	PROPN
biotropia-182	73	33	strains	strain	VERB
biotropia-182	73	34	,	,	PUNCT
biotropia-182	73	35	respectively	respectively	ADV
biotropia-182	73	36	.	.	PUNCT
biotropia-182	74	1	after	after	ADP
biotropia-182	74	2	placing	place	VERB
biotropia-182	74	3	3	3	NUM
biotropia-182	74	4	-	-	PUNCT
biotropia-182	74	5	mm	mm	NOUN
biotropia-182	74	6	diameter	diameter	NOUN
biotropia-182	74	7	agar	agar	NOUN
biotropia-182	74	8	disks	disk	NOUN
biotropia-182	74	9	of	of	ADP
biotropia-182	74	10	an	an	DET
biotropia-182	74	11	actively	actively	ADV
biotropia-182	74	12	growing	grow	VERB
biotropia-182	74	13	fungal	fungal	ADJ
biotropia-182	74	14	culture	culture	NOUN
biotropia-182	74	15	of	of	ADP
biotropia-182	74	16	either	either	DET
biotropia-182	74	17	botrytis	botrytis	NOUN
biotropia-182	74	18	cinerea	cinerea	NOUN
biotropia-182	74	19	tom98ld	tom98ld	NOUN
biotropia-182	74	20	and	and	CCONJ
biotropia-182	74	21	fusarium	fusarium	NOUN
biotropia-182	74	22	solani	solani	PROPN
biotropia-182	74	23	f.	f.	PROPN
biotropia-182	74	24	sp	sp	PROPN
biotropia-182	74	25	.	.	PROPN
biotropia-182	74	26	pisi	pisi	X
biotropia-182	74	27	,	,	PUNCT
biotropia-182	74	28	at	at	ADP
biotropia-182	74	29	3	3	NUM
biotropia-182	74	30	to	to	PART
biotropia-182	74	31	4	4	NUM
biotropia-182	74	32	cm	cm	NOUN
biotropia-182	74	33	away	away	ADV
biotropia-182	74	34	from	from	ADP
biotropia-182	74	35	each	each	DET
biotropia-182	74	36	side	side	NOUN
biotropia-182	74	37	of	of	ADP
biotropia-182	74	38	the	the	DET
biotropia-182	74	39	bacterial	bacterial	ADJ
biotropia-182	74	40	growth	growth	NOUN
biotropia-182	74	41	area	area	NOUN
biotropia-182	74	42	,	,	PUNCT
biotropia-182	74	43	the	the	DET
biotropia-182	74	44	plates	plate	NOUN
biotropia-182	74	45	were	be	AUX
biotropia-182	74	46	incubated	incubate	VERB
biotropia-182	74	47	for	for	ADP
biotropia-182	74	48	3	3	NUM
biotropia-182	74	49	to	to	PART
biotropia-182	74	50	25	25	NUM
biotropia-182	74	51	days	day	NOUN
biotropia-182	74	52	,	,	PUNCT
biotropia-182	74	53	until	until	SCONJ
biotropia-182	74	54	mycelium	mycelium	NOUN
biotropia-182	74	55	growing	grow	VERB
biotropia-182	74	56	from	from	ADP
biotropia-182	74	57	the	the	DET
biotropia-182	74	58	two	two	NUM
biotropia-182	74	59	sides	side	NOUN
biotropia-182	74	60	on	on	ADP
biotropia-182	74	61	a	a	DET
biotropia-182	74	62	control	control	NOUN
biotropia-182	74	63	plate	plate	NOUN
biotropia-182	74	64	came	come	VERB
biotropia-182	74	65	into	into	ADP
biotropia-182	74	66	contact	contact	NOUN
biotropia-182	74	67	.	.	PUNCT
biotropia-182	75	1	results	result	VERB
biotropia-182	75	2	construction	construction	NOUN
biotropia-182	75	3	of	of	ADP
biotropia-182	75	4	sail	sail	NOUN
biotropia-182	75	5	-	-	PUNCT
biotropia-182	75	6	cassette	cassette	NOUN
biotropia-182	75	7	ofchi	ofchi	NOUN
biotropia-182	75	8	constitutive	constitutive	ADJ
biotropia-182	75	9	promoter	promoter	NOUN
biotropia-182	75	10	of	of	ADP
biotropia-182	75	11	kanamycin	kanamycin	PROPN
biotropia-182	75	12	resistance	resistance	NOUN
biotropia-182	75	13	gene	gene	NOUN
biotropia-182	75	14	(	(	PUNCT
biotropia-182	75	15	pkmr	pkmr	NOUN
biotropia-182	75	16	)	)	PUNCT
biotropia-182	75	17	from	from	ADP
biotropia-182	75	18	tn903	tn903	PROPN
biotropia-182	75	19	used	use	VERB
biotropia-182	75	20	in	in	ADP
biotropia-182	75	21	this	this	DET
biotropia-182	75	22	study	study	NOUN
biotropia-182	75	23	possesses	possess	VERB
biotropia-182	75	24	a	a	DET
biotropia-182	75	25	unique	unique	ADJ
biotropia-182	75	26	restriction	restriction	NOUN
biotropia-182	75	27	site	site	NOUN
biotropia-182	75	28	,	,	PUNCT
biotropia-182	75	29	xhol	xhol	PROPN
biotropia-182	75	30	(	(	PUNCT
biotropia-182	75	31	oka	oka	NOUN
biotropia-182	75	32	et	et	PROPN
biotropia-182	75	33	al	al	PROPN
biotropia-182	75	34	.	.	PROPN
biotropia-182	75	35	1981	1981	NUM
biotropia-182	75	36	)	)	PUNCT
biotropia-182	75	37	.	.	PUNCT
biotropia-182	76	1	this	this	DET
biotropia-182	76	2	xhol	xhol	PROPN
biotropia-182	76	3	site	site	NOUN
biotropia-182	76	4	was	be	AUX
biotropia-182	76	5	employed	employ	VERB
biotropia-182	76	6	to	to	PART
biotropia-182	76	7	construct	construct	VERB
biotropia-182	76	8	a	a	DET
biotropia-182	76	9	transcriptional	transcriptional	ADJ
biotropia-182	76	10	fusion	fusion	NOUN
biotropia-182	76	11	of	of	ADP
biotropia-182	76	12	a.	a.	NOUN
biotropia-182	76	13	caviae	caviae	PROPN
biotropia-182	76	14	ws7b	ws7b	PROPN
biotropia-182	76	15	chitinase	chitinase	NOUN
biotropia-182	76	16	gene	gene	NOUN
biotropia-182	76	17	chi	chi	NOUN
biotropia-182	76	18	(	(	PUNCT
biotropia-182	76	19	accession	accession	NOUN
biotropia-182	76	20	number	number	NOUN
biotropia-182	76	21	aj431785	aj431785	PROPN
biotropia-182	76	22	)	)	PUNCT
biotropia-182	76	23	under	under	ADP
biotropia-182	76	24	the	the	DET
biotropia-182	76	25	control	control	NOUN
biotropia-182	76	26	of	of	ADP
biotropia-182	76	27	pkmr	pkmr	PROPN
biotropia-182	76	28	(	(	PUNCT
biotropia-182	76	29	fig.l	fig.l	PROPN
biotropia-182	76	30	)	)	PUNCT
biotropia-182	76	31	.	.	PUNCT
biotropia-182	77	1	the	the	DET
biotropia-182	77	2	chi	chi	PROPN
biotropia-182	77	3	gene	gene	NOUN
biotropia-182	77	4	in	in	ADP
biotropia-182	77	5	pws506	pws506	PROPN
biotropia-182	77	6	was	be	AUX
biotropia-182	77	7	cloned	clone	VERB
biotropia-182	77	8	without	without	ADP
biotropia-182	77	9	its	its	PRON
biotropia-182	77	10	indigenous	indigenous	ADJ
biotropia-182	77	11	promoter	promoter	NOUN
biotropia-182	77	12	(	(	PUNCT
biotropia-182	77	13	malik	malik	PROPN
biotropia-182	77	14	et	et	PROPN
biotropia-182	77	15	al	al	PROPN
biotropia-182	77	16	.	.	PROPN
biotropia-182	78	1	in	in	ADP
biotropia-182	78	2	press	press	NOUN
biotropia-182	78	3	)	)	PUNCT
biotropia-182	78	4	.	.	PUNCT
biotropia-182	79	1	plasmid	plasmid	ADJ
biotropia-182	79	2	pam201	pam201	PROPN
biotropia-182	79	3	-	-	PUNCT
biotropia-182	79	4	a	a	PRON
biotropia-182	79	5	carrying	carry	VERB
biotropia-182	79	6	a	a	DET
biotropia-182	79	7	sail	sail	NOUN
biotropia-182	79	8	cassette	cassette	NOUN
biotropia-182	79	9	of	of	ADP
biotropia-182	79	10	chi	chi	NOUN
biotropia-182	79	11	was	be	AUX
biotropia-182	79	12	constructed	construct	VERB
biotropia-182	79	13	through	through	ADP
biotropia-182	79	14	several	several	ADJ
biotropia-182	79	15	intermediate	intermediate	ADJ
biotropia-182	79	16	-	-	PUNCT
biotropia-182	79	17	cloning	cloning	NOUN
biotropia-182	79	18	steps	step	NOUN
biotropia-182	79	19	(	(	PUNCT
biotropia-182	79	20	fig	fig	NOUN
biotropia-182	79	21	.	.	NOUN
biotropia-182	79	22	1	1	NUM
biotropia-182	79	23	)	)	PUNCT
biotropia-182	79	24	.	.	PUNCT
biotropia-182	80	1	the	the	DET
biotropia-182	80	2	first	first	ADJ
biotropia-182	80	3	step	step	NOUN
biotropia-182	80	4	was	be	AUX
biotropia-182	80	5	the	the	DET
biotropia-182	80	6	insertion	insertion	NOUN
biotropia-182	80	7	of	of	ADP
biotropia-182	80	8	trimethoprim	trimethoprim	ADJ
biotropia-182	80	9	resistance	resistance	NOUN
biotropia-182	80	10	gene	gene	NOUN
biotropia-182	80	11	(	(	PUNCT
biotropia-182	80	12	tpr	tpr	NOUN
biotropia-182	80	13	)	)	PUNCT
biotropia-182	80	14	isolated	isolate	VERB
biotropia-182	80	15	from	from	ADP
biotropia-182	80	16	pass	pass	PROPN
biotropia-182	80	17	96	96	NUM
biotropia-182	80	18	(	(	PUNCT
biotropia-182	80	19	suwanto	suwanto	NOUN
biotropia-182	80	20	and	and	CCONJ
biotropia-182	80	21	kaplan	kaplan	PROPN
biotropia-182	80	22	1992	1992	NUM
biotropia-182	80	23	)	)	PUNCT
biotropia-182	80	24	,	,	PUNCT
biotropia-182	80	25	as	as	ADP
biotropia-182	80	26	a	a	DET
biotropia-182	80	27	marker	marker	NOUN
biotropia-182	80	28	downstream	downstream	ADJ
biotropia-182	80	29	ofchi	ofchi	NOUN
biotropia-182	80	30	fragment	fragment	NOUN
biotropia-182	80	31	at	at	ADP
biotropia-182	80	32	hpal	hpal	ADJ
biotropia-182	80	33	site	site	NOUN
biotropia-182	80	34	in	in	ADP
biotropia-182	80	35	pws506	pws506	PROPN
biotropia-182	80	36	.	.	PUNCT
biotropia-182	81	1	the	the	DET
biotropia-182	81	2	chi	chi	PROPN
biotropia-182	81	3	-	-	PUNCT
biotropia-182	81	4	tpr	tpr	PROPN
biotropia-182	81	5	fragment	fragment	NOUN
biotropia-182	81	6	was	be	AUX
biotropia-182	81	7	then	then	ADV
biotropia-182	81	8	isolated	isolate	VERB
biotropia-182	81	9	subsequently	subsequently	ADV
biotropia-182	81	10	employing	employ	VERB
biotropia-182	81	11	hindhl+ecorv	hindhl+ecorv	PROPN
biotropia-182	81	12	double	double	ADJ
biotropia-182	81	13	digestion	digestion	NOUN
biotropia-182	81	14	.	.	PUNCT
biotropia-182	82	1	the	the	DET
biotropia-182	82	2	protruding	protrude	VERB
biotropia-182	82	3	end	end	NOUN
biotropia-182	82	4	of	of	ADP
biotropia-182	82	5	hinalll	hinalll	NOUN
biotropia-182	82	6	was	be	AUX
biotropia-182	82	7	made	make	VERB
biotropia-182	82	8	blunt	blunt	ADJ
biotropia-182	82	9	by	by	ADP
biotropia-182	82	10	means	mean	NOUN
biotropia-182	82	11	of	of	ADP
biotropia-182	82	12	t4	t4	PROPN
biotropia-182	82	13	dna	dna	PROPN
biotropia-182	82	14	polymerase	polymerase	NOUN
biotropia-182	82	15	to	to	PART
biotropia-182	82	16	obtain	obtain	VERB
biotropia-182	82	17	blunt	blunt	ADJ
biotropia-182	82	18	end	end	NOUN
biotropia-182	82	19	chi	chi	ADJ
biotropia-182	82	20	-	-	PUNCT
biotropia-182	82	21	tpr	tpr	NOUN
biotropia-182	82	22	fragment	fragment	NOUN
biotropia-182	82	23	,	,	PUNCT
biotropia-182	82	24	which	which	PRON
biotropia-182	82	25	was	be	AUX
biotropia-182	82	26	then	then	ADV
biotropia-182	82	27	ligated	ligate	VERB
biotropia-182	82	28	with	with	ADP
biotropia-182	82	29	blunt	blunt	ADJ
biotropia-182	82	30	-	-	PUNCT
biotropia-182	82	31	end	end	NOUN
biotropia-182	82	32	hpal	hpal	NOUN
biotropia-182	82	33	of	of	ADP
biotropia-182	82	34	vector	vector	NOUN
biotropia-182	82	35	pas385	pas385	PROPN
biotropia-182	82	36	(	(	PUNCT
biotropia-182	82	37	suwanto	suwanto	NOUN
biotropia-182	82	38	and	and	CCONJ
biotropia-182	82	39	kaplan	kaplan	PROPN
biotropia-182	82	40	1992	1992	NUM
biotropia-182	82	41	)	)	PUNCT
biotropia-182	82	42	,	,	PUNCT
biotropia-182	82	43	generated	generate	VERB
biotropia-182	82	44	recombinant	recombinant	ADJ
biotropia-182	82	45	plasmid	plasmid	ADJ
biotropia-182	82	46	pam201	pam201	PROPN
biotropia-182	82	47	(	(	PUNCT
biotropia-182	82	48	fig	fig	NOUN
biotropia-182	82	49	1	1	NUM
biotropia-182	82	50	)	)	PUNCT
biotropia-182	82	51	.	.	PUNCT
biotropia-182	83	1	plasmid	plasmid	ADJ
biotropia-182	83	2	pam201	pam201	PROPN
biotropia-182	83	3	-	-	PUNCT
biotropia-182	83	4	a	a	PRON
biotropia-182	83	5	carries	carry	VERB
biotropia-182	83	6	c	c	NOUN
biotropia-182	83	7	/	/	SYM
biotropia-182	83	8	z/-tor	z/-tor	NOUN
biotropia-182	83	9	figure	figure	NOUN
biotropia-182	83	10	1	1	NUM
biotropia-182	83	11	.	.	PUNCT
biotropia-182	83	12	schematic	schematic	ADJ
biotropia-182	83	13	structure	structure	NOUN
biotropia-182	83	14	of	of	ADP
biotropia-182	83	15	sail	sail	NOUN
biotropia-182	83	16	cassette	cassette	NOUN
biotropia-182	83	17	construction	construction	NOUN
biotropia-182	83	18	of	of	ADP
biotropia-182	83	19	chi	chi	NOUN
biotropia-182	83	20	.	.	PUNCT
biotropia-182	84	1	the	the	DET
biotropia-182	84	2	promoter	promoter	NOUN
biotropia-182	84	3	of	of	ADP
biotropia-182	84	4	kanamycin	kanamycin	PROPN
biotropia-182	84	5	resistance	resistance	NOUN
biotropia-182	84	6	gene	gene	NOUN
biotropia-182	84	7	(	(	PUNCT
biotropia-182	84	8	pkmr	pkmr	NOUN
biotropia-182	84	9	)	)	PUNCT
biotropia-182	84	10	from	from	ADP
biotropia-182	84	11	tn903	tn903	VERB
biotropia-182	84	12	in	in	ADP
biotropia-182	84	13	puc4k	puc4k	PRON
biotropia-182	84	14	is	be	AUX
biotropia-182	84	15	located	locate	VERB
biotropia-182	84	16	upstream	upstream	NOUN
biotropia-182	84	17	of	of	ADP
biotropia-182	84	18	the	the	DET
biotropia-182	84	19	xhol	xhol	PROPN
biotropia-182	84	20	site	site	NOUN
biotropia-182	84	21	(	(	PUNCT
biotropia-182	84	22	oka	oka	NOUN
biotropia-182	84	23	et	et	NOUN
biotropia-182	84	24	a/,1981	a/,1981	NOUN
biotropia-182	84	25	)	)	PUNCT
biotropia-182	84	26	(	(	PUNCT
biotropia-182	84	27	a	a	NOUN
biotropia-182	84	28	)	)	PUNCT
biotropia-182	84	29	.	.	PUNCT
biotropia-182	85	1	a	a	DET
biotropia-182	85	2	trimethoprim	trimethoprim	ADJ
biotropia-182	85	3	resistance	resistance	NOUN
biotropia-182	85	4	gene	gene	NOUN
biotropia-182	85	5	(	(	PUNCT
biotropia-182	85	6	tpr	tpr	NOUN
biotropia-182	85	7	)	)	PUNCT
biotropia-182	85	8	fragment	fragment	NOUN
biotropia-182	85	9	,	,	PUNCT
biotropia-182	85	10	isolated	isolate	VERB
biotropia-182	85	11	from	from	ADP
biotropia-182	85	12	pas396	pas396	PROPN
biotropia-182	85	13	(	(	PUNCT
biotropia-182	85	14	suwanto	suwanto	PROPN
biotropia-182	85	15	&	&	CCONJ
biotropia-182	85	16	kaplan	kaplan	PROPN
biotropia-182	85	17	1992	1992	NUM
biotropia-182	85	18	)	)	PUNCT
biotropia-182	85	19	,	,	PUNCT
biotropia-182	85	20	was	be	AUX
biotropia-182	85	21	inserted	insert	VERB
biotropia-182	85	22	downstream	downstream	NOUN
biotropia-182	85	23	of	of	ADP
biotropia-182	85	24	the	the	DET
biotropia-182	85	25	chi	chi	ADJ
biotropia-182	85	26	fragment	fragment	NOUN
biotropia-182	85	27	in	in	ADP
biotropia-182	85	28	pws506	pws506	PROPN
biotropia-182	85	29	(	(	PUNCT
biotropia-182	85	30	malik	malik	PROPN
biotropia-182	85	31	et	et	PROPN
biotropia-182	85	32	al	al	PROPN
biotropia-182	85	33	.	.	PROPN
biotropia-182	86	1	in	in	ADP
biotropia-182	86	2	press	press	NOUN
biotropia-182	86	3	)	)	PUNCT
biotropia-182	86	4	as	as	ADP
biotropia-182	86	5	marker	marker	NOUN
biotropia-182	86	6	at	at	ADP
biotropia-182	86	7	hpal	hpal	ADJ
biotropia-182	86	8	site	site	NOUN
biotropia-182	86	9	in	in	ADP
biotropia-182	86	10	polyl	polyl	PROPN
biotropia-182	86	11	inker	inker	NOUN
biotropia-182	86	12	site	site	NOUN
biotropia-182	86	13	(	(	PUNCT
biotropia-182	86	14	b	b	NOUN
biotropia-182	86	15	)	)	PUNCT
biotropia-182	86	16	.	.	PUNCT
biotropia-182	87	1	the	the	DET
biotropia-182	87	2	c	c	NOUN
biotropia-182	87	3	/	/	SYM
biotropia-182	87	4	i/-tpr	i/-tpr	ADJ
biotropia-182	87	5	fragment	fragment	NOUN
biotropia-182	87	6	was	be	AUX
biotropia-182	87	7	isolated	isolate	VERB
biotropia-182	87	8	employing	employ	VERB
biotropia-182	87	9	hindlll+	hindlll+	X
biotropia-182	87	10	ecorv	ecorv	NOUN
biotropia-182	87	11	double	double	ADJ
biotropia-182	87	12	digestion	digestion	NOUN
biotropia-182	87	13	.	.	PUNCT
biotropia-182	88	1	the	the	DET
biotropia-182	88	2	protruding	protrude	VERB
biotropia-182	88	3	end	end	NOUN
biotropia-182	88	4	of	of	ADP
biotropia-182	88	5	ifiruh.ll	ifiruh.ll	NOUN
biotropia-182	88	6	was	be	AUX
biotropia-182	88	7	made	make	VERB
biotropia-182	88	8	blunt	blunt	ADJ
biotropia-182	88	9	,	,	PUNCT
biotropia-182	88	10	which	which	PRON
biotropia-182	88	11	was	be	AUX
biotropia-182	88	12	then	then	ADV
biotropia-182	88	13	ligated	ligate	VERB
biotropia-182	88	14	with	with	ADP
biotropia-182	88	15	blunt	blunt	ADJ
biotropia-182	88	16	-	-	PUNCT
biotropia-182	88	17	end	end	NOUN
biotropia-182	88	18	hpal	hpal	ADJ
biotropia-182	88	19	end	end	NOUN
biotropia-182	88	20	of	of	ADP
biotropia-182	88	21	linearized	linearize	VERB
biotropia-182	88	22	vector	vector	NOUN
biotropia-182	88	23	pas385	pas385	PROPN
biotropia-182	88	24	(	(	PUNCT
biotropia-182	88	25	suwanto	suwanto	NOUN
biotropia-182	88	26	&	&	CCONJ
biotropia-182	88	27	kaplon	kaplon	PROPN
biotropia-182	88	28	1992	1992	NUM
biotropia-182	88	29	)	)	PUNCT
biotropia-182	88	30	,	,	PUNCT
biotropia-182	88	31	resulting	result	VERB
biotropia-182	88	32	in	in	ADP
biotropia-182	88	33	recombinant	recombinant	ADJ
biotropia-182	88	34	plasmids	plasmid	NOUN
biotropia-182	88	35	pam201	pam201	NOUN
biotropia-182	88	36	-	-	PUNCT
biotropia-182	88	37	a	a	DET
biotropia-182	88	38	(	(	PUNCT
biotropia-182	88	39	chi+	chi+	NOUN
biotropia-182	88	40	)	)	PUNCT
biotropia-182	88	41	and	and	CCONJ
biotropia-182	88	42	pam201	pam201	NOUN
biotropia-182	88	43	-	-	PUNCT
biotropia-182	88	44	b	b	NOUN
biotropia-182	88	45	(	(	PUNCT
biotropia-182	88	46	chi-	chi-	INTJ
biotropia-182	88	47	)	)	PUNCT
biotropia-182	88	48	that	that	PRON
biotropia-182	88	49	carry	carry	VERB
biotropia-182	88	50	sail	sail	NOUN
biotropia-182	88	51	cassette	cassette	NOUN
biotropia-182	88	52	of	of	ADP
biotropia-182	88	53	c	c	NOUN
biotropia-182	88	54	/	/	SYM
biotropia-182	88	55	i;-tpr	i;-tpr	NOUN
biotropia-182	88	56	(	(	PUNCT
biotropia-182	88	57	c	c	NOUN
biotropia-182	88	58	)	)	PUNCT
biotropia-182	88	59	.	.	PUNCT
biotropia-182	89	1	heterologous	heterologous	ADJ
biotropia-182	89	2	expression	expression	NOUN
biotropia-182	89	3	of	of	ADP
biotropia-182	89	4	a	a	DET
biotropia-182	89	5	chitinase	chitinase	NOUN
biotropia-182	89	6	gene	gene	NOUN
biotropia-182	89	7	from	from	ADP
biotropia-182	89	8	aeromonas	aeromonas	PROPN
biotropia-182	89	9	caviae	caviae	PROPN
biotropia-182	89	10	amarila	amarila	PROPN
biotropia-182	89	11	malik	malik	PROPN
biotropia-182	89	12	et	et	PROPN
biotropia-182	89	13	al	al	PROPN
biotropia-182	89	14	.	.	PUNCT
biotropia-182	90	1	construction	construction	NOUN
biotropia-182	90	2	of	of	ADP
biotropia-182	90	3	pkmr	pkmr	NOUN
biotropia-182	90	4	-	-	PUNCT
biotropia-182	90	5	chi	chi	ADJ
biotropia-182	90	6	transcriptional	transcriptional	ADJ
biotropia-182	90	7	fusion	fusion	NOUN
biotropia-182	90	8	in	in	ADP
biotropia-182	90	9	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	90	10	a	a	DET
biotropia-182	90	11	medium	medium	ADJ
biotropia-182	90	12	copy	copy	NOUN
biotropia-182	90	13	number	number	NOUN
biotropia-182	90	14	broadhost	broadhost	NOUN
biotropia-182	90	15	-	-	PUNCT
biotropia-182	90	16	range	range	NOUN
biotropia-182	90	17	plasmid	plasmid	ADJ
biotropia-182	90	18	vector	vector	NOUN
biotropia-182	90	19	,	,	PUNCT
biotropia-182	90	20	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	90	21	(	(	PUNCT
biotropia-182	90	22	kovach	kovach	NOUN
biotropia-182	90	23	et	et	PROPN
biotropia-182	90	24	al	al	PROPN
biotropia-182	90	25	.	.	PROPN
biotropia-182	90	26	1994	1994	NUM
biotropia-182	90	27	)	)	PUNCT
biotropia-182	90	28	,	,	PUNCT
biotropia-182	90	29	was	be	AUX
biotropia-182	90	30	utilized	utilize	VERB
biotropia-182	90	31	as	as	ADP
biotropia-182	90	32	cloning	cloning	NOUN
biotropia-182	90	33	vehicle	vehicle	NOUN
biotropia-182	90	34	to	to	PART
biotropia-182	90	35	introduce	introduce	VERB
biotropia-182	90	36	pkmr	pkmr	ADJ
biotropia-182	90	37	-	-	PUNCT
biotropia-182	90	38	chi	chi	ADJ
biotropia-182	90	39	transcriptional	transcriptional	ADJ
biotropia-182	90	40	fusion	fusion	NOUN
biotropia-182	90	41	into	into	ADP
biotropia-182	90	42	pseudomonas	pseudomona	NOUN
biotropia-182	90	43	fluorescens	fluorescen	NOUN
biotropia-182	90	44	5100	5100	NUM
biotropia-182	90	45	.	.	PUNCT
biotropia-182	91	1	the	the	DET
biotropia-182	91	2	c/»/-tpr	c/»/-tpr	ADJ
biotropia-182	91	3	sail	sail	NOUN
biotropia-182	91	4	cassette	cassette	NOUN
biotropia-182	91	5	from	from	ADP
biotropia-182	91	6	pam201	pam201	NOUN
biotropia-182	91	7	-	-	PUNCT
biotropia-182	91	8	a	a	PRON
biotropia-182	91	9	was	be	AUX
biotropia-182	91	10	inserted	insert	VERB
biotropia-182	91	11	into	into	ADP
biotropia-182	91	12	xhol	xhol	PROPN
biotropia-182	91	13	site	site	NOUN
biotropia-182	91	14	of	of	ADP
biotropia-182	91	15	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	91	16	under	under	ADP
biotropia-182	91	17	pkmr	pkmr	NOUN
biotropia-182	91	18	to	to	PART
biotropia-182	91	19	generate	generate	VERB
biotropia-182	91	20	pam330	pam330	NOUN
biotropia-182	91	21	,	,	PUNCT
biotropia-182	91	22	in	in	ADP
biotropia-182	91	23	which	which	PRON
biotropia-182	91	24	c/»'-tpr	c/»'-tpr	PRON
biotropia-182	91	25	will	will	AUX
biotropia-182	91	26	be	be	AUX
biotropia-182	91	27	transcribed	transcribe	VERB
biotropia-182	91	28	under	under	ADP
biotropia-182	91	29	control	control	NOUN
biotropia-182	91	30	of	of	ADP
biotropia-182	91	31	kmr	kmr	PROPN
biotropia-182	91	32	promoter	promoter	NOUN
biotropia-182	91	33	(	(	PUNCT
biotropia-182	91	34	fig	fig	NOUN
biotropia-182	91	35	2	2	NUM
biotropia-182	91	36	)	)	PUNCT
biotropia-182	91	37	.	.	PUNCT
biotropia-182	92	1	bgli	bgli	PROPN
biotropia-182	92	2	figure	figure	NOUN
biotropia-182	92	3	2	2	NUM
biotropia-182	92	4	.	.	X
biotropia-182	92	5	restriction	restriction	NOUN
biotropia-182	92	6	map	map	NOUN
biotropia-182	92	7	of	of	ADP
biotropia-182	92	8	recombinant	recombinant	ADJ
biotropia-182	92	9	plasmid	plasmid	ADJ
biotropia-182	92	10	pam330	pam330	NOUN
biotropia-182	92	11	carrying	carry	VERB
biotropia-182	92	12	pkmr	pkmr	NOUN
biotropia-182	92	13	-	-	PUNCT
biotropia-182	92	14	c	c	NOUN
biotropia-182	92	15	/	/	SYM
biotropia-182	92	16	i	i	NOUN
biotropia-182	92	17	;	;	PUNCT
biotropia-182	92	18	transcriptional	transcriptional	ADJ
biotropia-182	92	19	fusion	fusion	NOUN
biotropia-182	92	20	in	in	ADP
biotropia-182	92	21	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	92	22	.	.	PUNCT
biotropia-182	93	1	the	the	DET
biotropia-182	93	2	additional	additional	ADJ
biotropia-182	93	3	gmr	gmr	NOUN
biotropia-182	93	4	marker	marker	NOUN
biotropia-182	93	5	was	be	AUX
biotropia-182	93	6	inserted	insert	VERB
biotropia-182	93	7	at	at	ADP
biotropia-182	93	8	///«diii	///«diii	PROPN
biotropia-182	93	9	site	site	NOUN
biotropia-182	93	10	downstream	downstream	NOUN
biotropia-182	93	11	of	of	ADP
biotropia-182	93	12	tpr	tpr	NOUN
biotropia-182	93	13	,	,	PUNCT
biotropia-182	93	14	which	which	PRON
biotropia-182	93	15	generated	generate	VERB
biotropia-182	93	16	pam340	pam340	NOUN
biotropia-182	93	17	in	in	ADP
biotropia-182	93	18	addition	addition	NOUN
biotropia-182	93	19	to	to	ADP
biotropia-182	93	20	tpr	tpr	PROPN
biotropia-182	93	21	marker	marker	NOUN
biotropia-182	93	22	,	,	PUNCT
biotropia-182	93	23	a	a	DET
biotropia-182	93	24	gmr	gmr	NOUN
biotropia-182	93	25	cassette	cassette	NOUN
biotropia-182	93	26	isolated	isolate	VERB
biotropia-182	93	27	from	from	ADP
biotropia-182	93	28	p34s	p34s	PROPN
biotropia-182	93	29	-	-	PUNCT
biotropia-182	93	30	gm	gm	PROPN
biotropia-182	93	31	(	(	PUNCT
biotropia-182	93	32	dennis	dennis	PROPN
biotropia-182	93	33	and	and	CCONJ
biotropia-182	93	34	zylstra	zylstra	NOUN
biotropia-182	93	35	1998	1998	NUM
biotropia-182	93	36	)	)	PUNCT
biotropia-182	93	37	was	be	AUX
biotropia-182	93	38	used	use	VERB
biotropia-182	93	39	as	as	ADP
biotropia-182	93	40	an	an	DET
biotropia-182	93	41	alternative	alternative	ADJ
biotropia-182	93	42	marker	marker	NOUN
biotropia-182	93	43	by	by	ADP
biotropia-182	93	44	inserting	insert	VERB
biotropia-182	93	45	the	the	DET
biotropia-182	93	46	cassette	cassette	NOUN
biotropia-182	93	47	downstream	downstream	NOUN
biotropia-182	93	48	of	of	ADP
biotropia-182	93	49	tpr	tpr	NOUN
biotropia-182	93	50	resulting	result	VERB
biotropia-182	93	51	in	in	ADP
biotropia-182	93	52	pam340	pam340	PROPN
biotropia-182	93	53	(	(	PUNCT
biotropia-182	93	54	fig	fig	NOUN
biotropia-182	93	55	2	2	NUM
biotropia-182	93	56	)	)	PUNCT
biotropia-182	93	57	,	,	PUNCT
biotropia-182	93	58	since	since	SCONJ
biotropia-182	93	59	tpr	tpr	NOUN
biotropia-182	93	60	marker	marker	NOUN
biotropia-182	93	61	was	be	AUX
biotropia-182	93	62	not	not	PART
biotropia-182	93	63	sufficient	sufficient	ADJ
biotropia-182	93	64	to	to	PART
biotropia-182	93	65	select	select	VERB
biotropia-182	93	66	for	for	ADP
biotropia-182	93	67	pf	pf	PROPN
biotropia-182	93	68	exconjugants	exconjugant	NOUN
biotropia-182	93	69	after	after	ADP
biotropia-182	93	70	mobilizing	mobilize	VERB
biotropia-182	93	71	pam330	pam330	NOUN
biotropia-182	93	72	from	from	ADP
biotropia-182	93	73	the	the	DET
biotropia-182	93	74	e.	e.	PROPN
biotropia-182	93	75	coli	coli	PROPN
biotropia-182	93	76	transformant	transformant	NOUN
biotropia-182	93	77	.	.	PUNCT
biotropia-182	94	1	construction	construction	NOUN
biotropia-182	94	2	of	of	ADP
biotropia-182	94	3	ptac	ptac	NOUN
biotropia-182	94	4	-	-	ADJ
biotropia-182	94	5	chi	chi	ADJ
biotropia-182	94	6	transcriptional	transcriptional	ADJ
biotropia-182	94	7	fusion	fusion	NOUN
biotropia-182	94	8	in	in	ADP
biotropia-182	94	9	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	94	10	a	a	DET
biotropia-182	94	11	bamhl	bamhl	NOUN
biotropia-182	94	12	fragment	fragment	NOUN
biotropia-182	94	13	of	of	ADP
biotropia-182	94	14	ptac	ptac	NOUN
biotropia-182	94	15	isolated	isolate	VERB
biotropia-182	94	16	from	from	ADP
biotropia-182	94	17	pkk223	pkk223	PROPN
biotropia-182	94	18	-	-	PUNCT
biotropia-182	94	19	3	3	NUM
biotropia-182	94	20	was	be	AUX
biotropia-182	94	21	inserted	insert	VERB
biotropia-182	94	22	into	into	ADP
biotropia-182	94	23	bamhl	bamhl	NOUN
biotropia-182	94	24	site	site	NOUN
biotropia-182	94	25	in	in	ADP
biotropia-182	94	26	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	94	27	,	,	PUNCT
biotropia-182	94	28	generating	generate	VERB
biotropia-182	94	29	plasmid	plasmid	ADJ
biotropia-182	94	30	pam601	pam601	PROPN
biotropia-182	94	31	.	.	PUNCT
biotropia-182	95	1	chitinase	chitinase	PROPN
biotropia-182	95	2	gene	gene	NOUN
biotropia-182	95	3	chi	chi	PROPN
biotropia-182	95	4	,	,	PUNCT
biotropia-182	95	5	which	which	PRON
biotropia-182	95	6	was	be	AUX
biotropia-182	95	7	isolated	isolate	VERB
biotropia-182	95	8	from	from	ADP
biotropia-182	95	9	pam202	pam202	PROPN
biotropia-182	95	10	as	as	ADP
biotropia-182	95	11	hindlll	hindlll	NOUN
biotropia-182	95	12	-	-	PUNCT
biotropia-182	95	13	ecorl	ecorl	NOUN
biotropia-182	95	14	c	c	NOUN
biotropia-182	95	15	/	/	SYM
biotropia-182	95	16	i/-gmr	i/-gmr	ADJ
biotropia-182	95	17	fragment	fragment	NOUN
biotropia-182	95	18	,	,	PUNCT
biotropia-182	95	19	was	be	AUX
biotropia-182	95	20	inserted	insert	VERB
biotropia-182	95	21	subsequently	subsequently	ADV
biotropia-182	95	22	into	into	ADP
biotropia-182	95	23	this	this	DET
biotropia-182	95	24	plasmid	plasmid	ADJ
biotropia-182	95	25	vector	vector	NOUN
biotropia-182	95	26	,	,	PUNCT
biotropia-182	95	27	resulting	result	VERB
biotropia-182	95	28	in	in	ADP
biotropia-182	95	29	recombinant	recombinant	ADJ
biotropia-182	95	30	transcriptional	transcriptional	ADJ
biotropia-182	95	31	fusion	fusion	NOUN
biotropia-182	95	32	plasmid	plasmid	NOUN
biotropia-182	95	33	harboring	harbor	VERB
biotropia-182	95	34	ptac	ptac	NOUN
biotropia-182	95	35	-	-	PUNCT
biotropia-182	95	36	chi	chi	ADJ
biotropia-182	95	37	,	,	PUNCT
biotropia-182	95	38	designated	designate	VERB
biotropia-182	95	39	as	as	ADP
biotropia-182	95	40	pam630	pam630	PROPN
biotropia-182	95	41	(	(	PUNCT
biotropia-182	95	42	fig	fig	NOUN
biotropia-182	95	43	3	3	NUM
biotropia-182	95	44	)	)	PUNCT
biotropia-182	95	45	.	.	PUNCT
biotropia-182	96	1	plasmid	plasmid	NOUN
biotropia-182	96	2	pam202	pam202	PROPN
biotropia-182	96	3	was	be	AUX
biotropia-182	96	4	generated	generate	VERB
biotropia-182	96	5	from	from	ADP
biotropia-182	96	6	pam201	pam201	PROPN
biotropia-182	96	7	-	-	PUNCT
biotropia-182	96	8	b	b	NOUN
biotropia-182	96	9	by	by	ADP
biotropia-182	96	10	replacing	replace	VERB
biotropia-182	96	11	tpr	tpr	NOUN
biotropia-182	96	12	with	with	ADP
biotropia-182	96	13	gmr	gmr	PROPN
biotropia-182	96	14	.	.	PROPN
biotropia-182	96	15	biotropia	biotropia	PROPN
biotropia-182	96	16	no	no	PROPN
biotropia-182	96	17	.	.	PROPN
biotropia-182	96	18	20	20	NUM
biotropia-182	96	19	.	.	X
biotropia-182	97	1	2003	2003	NUM
biotropia-182	97	2	ecori	ecori	PROPN
biotropia-182	97	3	hind	hind	PROPN
biotropia-182	97	4	iii	iii	PROPN
biotropia-182	97	5	.	.	PUNCT
biotropia-182	97	6	abbreviations	abbreviation	NOUN
biotropia-182	97	7	:	:	PUNCT
biotropia-182	97	8	km	km	NOUN
biotropia-182	97	9	"	"	PUNCT
biotropia-182	97	10	=	=	PUNCT
biotropia-182	97	11	kanamycin	kanamycin	PROPN
biotropia-182	97	12	resistance	resistance	NOUN
biotropia-182	97	13	gene	gene	NOUN
biotropia-182	97	14	;	;	PUNCT
biotropia-182	97	15	pkmr	pkmr	NOUN
biotropia-182	97	16	=	=	SYM
biotropia-182	97	17	promotor	promotor	NOUN
biotropia-182	97	18	of	of	ADP
biotropia-182	97	19	kanamycin	kanamycin	PROPN
biotropia-182	97	20	resistance	resistance	NOUN
biotropia-182	97	21	gene	gene	NOUN
biotropia-182	97	22	;	;	PUNCT
biotropia-182	97	23	apr	apr	PROPN
biotropia-182	97	24	=	=	SYM
biotropia-182	97	25	ampicillin	ampicillin	PROPN
biotropia-182	97	26	resistance	resistance	NOUN
biotropia-182	97	27	gene	gene	NOUN
biotropia-182	97	28	;	;	PUNCT
biotropia-182	97	29	tp	tp	PART
biotropia-182	97	30	"	"	PUNCT
biotropia-182	97	31	=	=	PUNCT
biotropia-182	97	32	trimethoprim	trimethoprim	NOUN
biotropia-182	97	33	resistance	resistance	NOUN
biotropia-182	97	34	gene	gene	NOUN
biotropia-182	97	35	;	;	PUNCT
biotropia-182	97	36	gmr	gmr	PROPN
biotropia-182	97	37	=	=	PUNCT
biotropia-182	97	38	gentamycin	gentamycin	VERB
biotropia-182	97	39	resistance	resistance	NOUN
biotropia-182	97	40	gene	gene	NOUN
biotropia-182	97	41	;	;	PUNCT
biotropia-182	97	42	chi	chi	NOUN
biotropia-182	97	43	=	=	SYM
biotropia-182	97	44	chitinase	chitinase	NOUN
biotropia-182	97	45	gene	gene	NOUN
biotropia-182	97	46	figure	figure	NOUN
biotropia-182	97	47	3	3	NUM
biotropia-182	97	48	.	.	X
biotropia-182	97	49	restriction	restriction	NOUN
biotropia-182	97	50	map	map	NOUN
biotropia-182	97	51	of	of	ADP
biotropia-182	97	52	recombinant	recombinant	ADJ
biotropia-182	97	53	plasmid	plasmid	ADJ
biotropia-182	97	54	pam630	pam630	NOUN
biotropia-182	97	55	carrying	carry	VERB
biotropia-182	97	56	ftac	ftac	ADJ
biotropia-182	97	57	—	—	PUNCT
biotropia-182	97	58	chi	chi	ADJ
biotropia-182	97	59	transcriptional	transcriptional	ADJ
biotropia-182	97	60	fusion	fusion	NOUN
biotropia-182	97	61	in	in	ADP
biotropia-182	97	62	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	97	63	chitinase	chitinase	NOUN
biotropia-182	97	64	expression	expression	NOUN
biotropia-182	97	65	of	of	ADP
biotropia-182	97	66	transcriptional	transcriptional	ADJ
biotropia-182	97	67	fusions	fusion	NOUN
biotropia-182	97	68	in	in	ADP
biotropia-182	97	69	e.	e.	PROPN
biotropia-182	97	70	coli	coli	PROPN
biotropia-182	97	71	and	and	CCONJ
biotropia-182	97	72	p.	p.	NOUN
biotropia-182	97	73	fluorescens	fluorescen	NOUN
biotropia-182	97	74	.	.	PUNCT
biotropia-182	98	1	screening	screen	VERB
biotropia-182	98	2	of	of	ADP
biotropia-182	98	3	e.	e.	PROPN
biotropia-182	98	4	coli	coli	PROPN
biotropia-182	98	5	transformants	transformant	NOUN
biotropia-182	98	6	harboring	harbor	VERB
biotropia-182	98	7	pam340	pam340	NOUN
biotropia-182	98	8	,	,	PUNCT
biotropia-182	98	9	or	or	CCONJ
biotropia-182	98	10	pam630	pam630	NOUN
biotropia-182	98	11	on	on	ADP
biotropia-182	98	12	chitin	chitin	NOUN
biotropia-182	98	13	agar	agar	NOUN
biotropia-182	98	14	plate	plate	NOUN
biotropia-182	98	15	showed	show	VERB
biotropia-182	98	16	only	only	ADV
biotropia-182	98	17	weak	weak	ADJ
biotropia-182	98	18	clearing	clearing	NOUN
biotropia-182	98	19	zones	zone	NOUN
biotropia-182	98	20	observed	observe	VERB
biotropia-182	98	21	after	after	ADP
biotropia-182	98	22	7	7	NUM
biotropia-182	98	23	and	and	CCONJ
biotropia-182	98	24	11	11	NUM
biotropia-182	98	25	days	day	NOUN
biotropia-182	98	26	of	of	ADP
biotropia-182	98	27	incubation	incubation	NOUN
biotropia-182	98	28	,	,	PUNCT
biotropia-182	98	29	respectively	respectively	ADV
biotropia-182	98	30	(	(	PUNCT
biotropia-182	98	31	table	table	NOUN
biotropia-182	98	32	2	2	NUM
biotropia-182	98	33	)	)	PUNCT
biotropia-182	98	34	.	.	PUNCT
biotropia-182	99	1	on	on	ADP
biotropia-182	99	2	the	the	DET
biotropia-182	99	3	other	other	ADJ
biotropia-182	99	4	hand	hand	NOUN
biotropia-182	99	5	,	,	PUNCT
biotropia-182	99	6	e.	e.	PROPN
biotropia-182	99	7	coli	coli	PROPN
biotropia-182	99	8	harboring	harboring	NOUN
biotropia-182	99	9	pws506	pws506	PROPN
biotropia-182	99	10	showed	show	VERB
biotropia-182	99	11	strong	strong	ADJ
biotropia-182	99	12	clearing	clearing	NOUN
biotropia-182	99	13	zones	zone	NOUN
biotropia-182	99	14	after	after	ADP
biotropia-182	99	15	only	only	ADV
biotropia-182	99	16	3	3	NUM
biotropia-182	99	17	days	day	NOUN
biotropia-182	99	18	of	of	ADP
biotropia-182	99	19	incubation	incubation	NOUN
biotropia-182	99	20	.	.	PUNCT
biotropia-182	100	1	table	table	NOUN
biotropia-182	100	2	2	2	NUM
biotropia-182	100	3	.	.	PUNCT
biotropia-182	100	4	chitinolytic	chitinolytic	ADJ
biotropia-182	100	5	expression	expression	NOUN
biotropia-182	100	6	of	of	ADP
biotropia-182	100	7	chi	chi	NOUN
biotropia-182	100	8	on	on	ADP
biotropia-182	100	9	chitin	chitin	NOUN
biotropia-182	100	10	agar	agar	NOUN
biotropia-182	100	11	plates	plate	NOUN
biotropia-182	100	12	.	.	PUNCT
biotropia-182	101	1	strains	strain	VERB
biotropia-182	101	2	expression	expression	NOUN
biotropia-182	101	3	chitinolytic	chitinolytic	ADJ
biotropia-182	101	4	activity	activity	NOUN
biotropia-182	101	5	control	control	NOUN
biotropia-182	101	6	as	as	ADP
biotropia-182	101	7	clearance	clearance	NOUN
biotropia-182	101	8	zone	zone	NOUN
biotropia-182	101	9	*	*	PUNCT
biotropia-182	101	10	incubation	incubation	NOUN
biotropia-182	101	11	time	time	NOUN
biotropia-182	101	12	(	(	PUNCT
biotropia-182	101	13	days	day	NOUN
biotropia-182	101	14	)	)	PUNCT
biotropia-182	101	15	e.	e.	PROPN
biotropia-182	101	16	co//top10(pws506	co//top10(pws506	PROPN
biotropia-182	101	17	)	)	PUNCT
biotropia-182	101	18	plac	plac	NOUN
biotropia-182	102	1	+	+	PROPN
biotropia-182	102	2	+	+	PROPN
biotropia-182	102	3	+	+	PROPN
biotropia-182	102	4	3	3	NUM
biotropia-182	102	5	e.	e.	PROPN
biotropia-182	102	6	co//top10(pam340	co//top10(pam340	PROPN
biotropia-182	102	7	)	)	PUNCT
biotropia-182	102	8	pkmr	pkmr	NOUN
biotropia-182	102	9	+	+	CCONJ
biotropia-182	102	10	7	7	NUM
biotropia-182	102	11	e.	e.	NOUN
biotropia-182	102	12	co//top10(pam630	co//top10(pam630	PROPN
biotropia-182	102	13	)	)	PUNCT
biotropia-182	102	14	ptoc	ptoc	NOUN
biotropia-182	102	15	+	+	CCONJ
biotropia-182	102	16	11	11	NUM
biotropia-182	102	17	p.	p.	NOUN
biotropia-182	102	18	fluorescens	fluorescen	NOUN
biotropia-182	102	19	5100	5100	NUM
biotropia-182	102	20	(	(	PUNCT
biotropia-182	102	21	pam340	pam340	NOUN
biotropia-182	102	22	)	)	PUNCT
biotropia-182	102	23	pkmr	pkmr	NOUN
biotropia-182	103	1	+	+	PROPN
biotropia-182	103	2	+	+	ADJ
biotropia-182	103	3	7	7	NUM
biotropia-182	103	4	p.	p.	NOUN
biotropia-182	103	5	fluorescens	fluorescen	NOUN
biotropia-182	103	6	5100	5100	NUM
biotropia-182	103	7	(	(	PUNCT
biotropia-182	103	8	pam630	pam630	NOUN
biotropia-182	103	9	)	)	PUNCT
biotropia-182	103	10	ptoc	ptoc	NOUN
biotropia-182	104	1	+	+	PROPN
biotropia-182	105	1	+	+	NUM
biotropia-182	105	2	11	11	NUM
biotropia-182	105	3	*	*	PUNCT
biotropia-182	105	4	relative	relative	ADJ
biotropia-182	105	5	chitin	chitin	NOUN
biotropia-182	105	6	degradation	degradation	NOUN
biotropia-182	105	7	ability	ability	NOUN
biotropia-182	105	8	was	be	AUX
biotropia-182	105	9	indicated	indicate	VERB
biotropia-182	105	10	as	as	ADP
biotropia-182	105	11	+	+	PROPN
biotropia-182	105	12	,	,	PUNCT
biotropia-182	105	13	+	+	PROPN
biotropia-182	105	14	+	+	NOUN
biotropia-182	105	15	and	and	CCONJ
biotropia-182	105	16	+	+	PROPN
biotropia-182	105	17	+	+	PROPN
biotropia-182	105	18	+	+	X
biotropia-182	105	19	for	for	ADP
biotropia-182	105	20	slightly	slightly	ADV
biotropia-182	105	21	,	,	PUNCT
biotropia-182	105	22	moderate	moderate	ADJ
biotropia-182	105	23	,	,	PUNCT
biotropia-182	105	24	and	and	CCONJ
biotropia-182	105	25	very	very	ADV
biotropia-182	105	26	clear	clear	ADJ
biotropia-182	105	27	zone	zone	NOUN
biotropia-182	105	28	,	,	PUNCT
biotropia-182	105	29	respectively	respectively	ADV
biotropia-182	105	30	.	.	PUNCT
biotropia-182	106	1	plasmid	plasmid	ADJ
biotropia-182	106	2	pam340	pam340	PROPN
biotropia-182	106	3	and	and	CCONJ
biotropia-182	106	4	pam630	pam630	PROPN
biotropia-182	106	5	were	be	AUX
biotropia-182	106	6	mobilized	mobilize	VERB
biotropia-182	106	7	into	into	ADP
biotropia-182	106	8	spontaneous	spontaneous	ADJ
biotropia-182	106	9	rifampicin	rifampicin	VERB
biotropia-182	106	10	resistance	resistance	NOUN
biotropia-182	106	11	(	(	PUNCT
biotropia-182	106	12	rif	rif	PROPN
biotropia-182	106	13	)	)	PUNCT
biotropia-182	106	14	mutants	mutant	NOUN
biotropia-182	106	15	of	of	ADP
biotropia-182	106	16	strain	strain	NOUN
biotropia-182	106	17	pf5100	pf5100	NOUN
biotropia-182	106	18	.	.	PUNCT
biotropia-182	107	1	screen	screen	PROPN
biotropia-182	107	2	ino	ino	PROPN
biotropia-182	107	3	fnr	fnr	PROPN
biotropia-182	107	4	pf	pf	PROPN
biotropia-182	107	5	phitmr.krf;/	phitmr.krf;/	PROPN
biotropia-182	107	6	heterologous	heterologous	ADJ
biotropia-182	107	7	expression	expression	NOUN
biotropia-182	107	8	of	of	ADP
biotropia-182	107	9	a	a	DET
biotropia-182	107	10	chitinase	chitinase	NOUN
biotropia-182	107	11	gene	gene	NOUN
biotropia-182	107	12	from	from	ADP
biotropia-182	107	13	aeromonas	aeromonas	PROPN
biotropia-182	107	14	caviae	caviae	PROPN
biotropia-182	107	15	—	—	PUNCT
biotropia-182	107	16	amarila	amarila	NOUN
biotropia-182	107	17	malik	malik	PROPN
biotropia-182	107	18	et	et	PROPN
biotropia-182	107	19	al	al	PROPN
biotropia-182	107	20	.	.	PROPN
biotropia-182	107	21	selection	selection	NOUN
biotropia-182	107	22	,	,	PUNCT
biotropia-182	107	23	in	in	ADP
biotropia-182	107	24	conjunction	conjunction	NOUN
biotropia-182	107	25	with	with	ADP
biotropia-182	107	26	pcr	pcr	ADJ
biotropia-182	107	27	techniques	technique	NOUN
biotropia-182	107	28	using	use	VERB
biotropia-182	107	29	chi	chi	ADJ
biotropia-182	107	30	primers	primer	NOUN
biotropia-182	107	31	as	as	SCONJ
biotropia-182	107	32	described	describe	VERB
biotropia-182	107	33	above	above	ADV
biotropia-182	107	34	.	.	PUNCT
biotropia-182	108	1	approximately	approximately	ADV
biotropia-182	108	2	800	800	NUM
biotropia-182	108	3	bp	bp	PROPN
biotropia-182	108	4	bands	band	NOUN
biotropia-182	108	5	were	be	AUX
biotropia-182	108	6	observed	observe	VERB
biotropia-182	108	7	on	on	ADP
biotropia-182	108	8	agarose	agarose	NOUN
biotropia-182	108	9	gel	gel	NOUN
biotropia-182	108	10	after	after	ADP
biotropia-182	108	11	electrophoresis	electrophoresis	NOUN
biotropia-182	108	12	of	of	ADP
biotropia-182	108	13	exconjugants	exconjugant	NOUN
biotropia-182	108	14	(	(	PUNCT
biotropia-182	108	15	data	datum	NOUN
biotropia-182	108	16	not	not	PART
biotropia-182	108	17	shown	show	VERB
biotropia-182	108	18	)	)	PUNCT
biotropia-182	108	19	.	.	PUNCT
biotropia-182	109	1	the	the	DET
biotropia-182	109	2	latter	latter	ADJ
biotropia-182	109	3	was	be	AUX
biotropia-182	109	4	a	a	DET
biotropia-182	109	5	rapid	rapid	ADJ
biotropia-182	109	6	and	and	CCONJ
biotropia-182	109	7	easy	easy	ADJ
biotropia-182	109	8	method	method	NOUN
biotropia-182	109	9	to	to	PART
biotropia-182	109	10	verify	verify	VERB
biotropia-182	109	11	the	the	DET
biotropia-182	109	12	exconjugants	exconjugant	NOUN
biotropia-182	109	13	after	after	ADP
biotropia-182	109	14	isolating	isolate	VERB
biotropia-182	109	15	single	single	ADJ
biotropia-182	109	16	colonies	colony	NOUN
biotropia-182	109	17	.	.	PUNCT
biotropia-182	110	1	constitutive	constitutive	ADJ
biotropia-182	110	2	chitinase	chitinase	NOUN
biotropia-182	110	3	expression	expression	NOUN
biotropia-182	110	4	of	of	ADP
biotropia-182	110	5	pkmr	pkmr	NOUN
biotropia-182	110	6	-	-	PUNCT
biotropia-182	110	7	chi	chi	NOUN
biotropia-182	110	8	the	the	DET
biotropia-182	110	9	modified	modify	VERB
biotropia-182	110	10	chitin	chitin	NOUN
biotropia-182	110	11	azure	azure	NOUN
biotropia-182	110	12	assay	assay	NOUN
biotropia-182	110	13	used	use	VERB
biotropia-182	110	14	in	in	ADP
biotropia-182	110	15	this	this	DET
biotropia-182	110	16	study	study	NOUN
biotropia-182	110	17	could	could	AUX
biotropia-182	110	18	demonstrate	demonstrate	VERB
biotropia-182	110	19	the	the	DET
biotropia-182	110	20	constitutive	constitutive	ADJ
biotropia-182	110	21	expression	expression	NOUN
biotropia-182	110	22	of	of	ADP
biotropia-182	110	23	the	the	DET
biotropia-182	110	24	cloned	cloned	ADJ
biotropia-182	110	25	chitinase	chitinase	NOUN
biotropia-182	110	26	gene	gene	NOUN
biotropia-182	110	27	by	by	ADP
biotropia-182	110	28	growing	grow	VERB
biotropia-182	110	29	the	the	DET
biotropia-182	110	30	e.	e.	PROPN
biotropia-182	110	31	coli	coli	PROPN
biotropia-182	110	32	recombinant	recombinant	NOUN
biotropia-182	110	33	and	and	CCONJ
biotropia-182	110	34	pf	pf	NOUN
biotropia-182	110	35	exconjugant	exconjugant	NOUN
biotropia-182	110	36	without	without	ADP
biotropia-182	110	37	chitin	chitin	NOUN
biotropia-182	110	38	.	.	PUNCT
biotropia-182	111	1	the	the	DET
biotropia-182	111	2	colloidal	colloidal	NOUN
biotropia-182	111	3	form	form	NOUN
biotropia-182	111	4	of	of	ADP
biotropia-182	111	5	chitin	chitin	NOUN
biotropia-182	111	6	azure	azure	NOUN
biotropia-182	111	7	,	,	PUNCT
biotropia-182	111	8	which	which	PRON
biotropia-182	111	9	consists	consist	VERB
biotropia-182	111	10	of	of	ADP
biotropia-182	111	11	oligomers	oligomer	NOUN
biotropia-182	111	12	and	and	CCONJ
biotropia-182	111	13	monomers	monomer	NOUN
biotropia-182	111	14	,	,	PUNCT
biotropia-182	111	15	was	be	AUX
biotropia-182	111	16	fairly	fairly	ADV
biotropia-182	111	17	easy	easy	ADJ
biotropia-182	111	18	to	to	PART
biotropia-182	111	19	degrade	degrade	VERB
biotropia-182	111	20	by	by	ADP
biotropia-182	111	21	chitinolytic	chitinolytic	ADJ
biotropia-182	111	22	activity	activity	NOUN
biotropia-182	111	23	compared	compare	VERB
biotropia-182	111	24	to	to	ADP
biotropia-182	111	25	the	the	DET
biotropia-182	111	26	flake	flake	NOUN
biotropia-182	111	27	form	form	NOUN
biotropia-182	111	28	of	of	ADP
biotropia-182	111	29	this	this	DET
biotropia-182	111	30	compound	compound	NOUN
biotropia-182	111	31	.	.	PUNCT
biotropia-182	112	1	we	we	PRON
biotropia-182	112	2	also	also	ADV
biotropia-182	112	3	constructed	construct	VERB
biotropia-182	112	4	a	a	DET
biotropia-182	112	5	transcriptional	transcriptional	ADJ
biotropia-182	112	6	fusion	fusion	NOUN
biotropia-182	112	7	of	of	ADP
biotropia-182	112	8	the	the	DET
biotropia-182	112	9	same	same	ADJ
biotropia-182	112	10	chitinase	chitinase	NOUN
biotropia-182	112	11	gene	gene	NOUN
biotropia-182	112	12	under	under	ADP
biotropia-182	112	13	the	the	DET
biotropia-182	112	14	tac	tac	PROPN
biotropia-182	112	15	promoter	promoter	NOUN
biotropia-182	112	16	(	(	PUNCT
biotropia-182	112	17	fig	fig	NOUN
biotropia-182	112	18	.	.	PUNCT
biotropia-182	113	1	3	3	NUM
biotropia-182	113	2	)	)	PUNCT
biotropia-182	113	3	,	,	PUNCT
biotropia-182	113	4	in	in	ADP
biotropia-182	113	5	order	order	NOUN
biotropia-182	113	6	to	to	PART
biotropia-182	113	7	compare	compare	VERB
biotropia-182	113	8	the	the	DET
biotropia-182	113	9	promoter	promoter	NOUN
biotropia-182	113	10	activity	activity	NOUN
biotropia-182	113	11	.	.	PUNCT
biotropia-182	114	1	expression	expression	NOUN
biotropia-182	114	2	assays	assay	NOUN
biotropia-182	114	3	of	of	ADP
biotropia-182	114	4	both	both	DET
biotropia-182	114	5	transcriptional	transcriptional	ADJ
biotropia-182	114	6	fusions	fusion	NOUN
biotropia-182	114	7	were	be	AUX
biotropia-182	114	8	carried	carry	VERB
biotropia-182	114	9	out	out	ADP
biotropia-182	114	10	under	under	ADP
biotropia-182	114	11	uninduced	uninduced	ADJ
biotropia-182	114	12	growing	grow	VERB
biotropia-182	114	13	conditions	condition	NOUN
biotropia-182	114	14	.	.	PUNCT
biotropia-182	115	1	the	the	DET
biotropia-182	115	2	result	result	NOUN
biotropia-182	115	3	showed	show	VERB
biotropia-182	115	4	that	that	SCONJ
biotropia-182	115	5	constitutive	constitutive	ADJ
biotropia-182	115	6	expression	expression	NOUN
biotropia-182	115	7	of	of	ADP
biotropia-182	115	8	chi	chi	PROPN
biotropia-182	115	9	under	under	ADP
biotropia-182	115	10	pkmr	pkmr	NOUN
biotropia-182	115	11	was	be	AUX
biotropia-182	115	12	slightly	slightly	ADV
biotropia-182	115	13	stronger	strong	ADJ
biotropia-182	115	14	(	(	PUNCT
biotropia-182	115	15	tables	table	NOUN
biotropia-182	115	16	2	2	NUM
biotropia-182	115	17	and	and	CCONJ
biotropia-182	115	18	3	3	NUM
biotropia-182	115	19	)	)	PUNCT
biotropia-182	115	20	.	.	PUNCT
biotropia-182	116	1	by	by	ADP
biotropia-182	116	2	comparing	compare	VERB
biotropia-182	116	3	chitinolytic	chitinolytic	ADJ
biotropia-182	116	4	activity	activity	NOUN
biotropia-182	116	5	of	of	ADP
biotropia-182	116	6	these	these	DET
biotropia-182	116	7	two	two	NUM
biotropia-182	116	8	p.	p.	NOUN
biotropia-182	116	9	fluorescens	fluorescen	NOUN
biotropia-182	116	10	5100	5100	NUM
biotropia-182	116	11	exconjugants	exconjugant	NOUN
biotropia-182	116	12	to	to	ADP
biotropia-182	116	13	the	the	DET
biotropia-182	116	14	wild	wild	ADJ
biotropia-182	116	15	type	type	NOUN
biotropia-182	116	16	bacterium	bacterium	NOUN
biotropia-182	116	17	a.	a.	NOUN
biotropia-182	116	18	caviae	caviae	PROPN
biotropia-182	116	19	ws7b	ws7b	PROPN
biotropia-182	116	20	(	(	PUNCT
biotropia-182	116	21	the	the	DET
biotropia-182	116	22	origin	origin	NOUN
biotropia-182	116	23	of	of	ADP
biotropia-182	116	24	chi	chi	ADJ
biotropia-182	116	25	gene	gene	NOUN
biotropia-182	116	26	in	in	ADP
biotropia-182	116	27	this	this	DET
biotropia-182	116	28	study	study	NOUN
biotropia-182	116	29	)	)	PUNCT
biotropia-182	116	30	,	,	PUNCT
biotropia-182	116	31	it	it	PRON
biotropia-182	116	32	could	could	AUX
biotropia-182	116	33	be	be	AUX
biotropia-182	116	34	demonstrated	demonstrate	VERB
biotropia-182	116	35	that	that	SCONJ
biotropia-182	116	36	constitutive	constitutive	ADJ
biotropia-182	116	37	chitinolytic	chitinolytic	ADJ
biotropia-182	116	38	activity	activity	NOUN
biotropia-182	116	39	of	of	ADP
biotropia-182	116	40	these	these	DET
biotropia-182	116	41	two	two	NUM
biotropia-182	116	42	strains	strain	NOUN
biotropia-182	116	43	of	of	ADP
biotropia-182	116	44	p.	p.	NOUN
biotropia-182	116	45	fluorescens	fluorescen	NOUN
biotropia-182	116	46	were	be	AUX
biotropia-182	116	47	less	less	ADV
biotropia-182	116	48	active	active	ADJ
biotropia-182	116	49	.	.	PUNCT
biotropia-182	117	1	this	this	PRON
biotropia-182	117	2	is	be	AUX
biotropia-182	117	3	consistent	consistent	ADJ
biotropia-182	117	4	with	with	ADP
biotropia-182	117	5	the	the	DET
biotropia-182	117	6	result	result	NOUN
biotropia-182	117	7	of	of	ADP
biotropia-182	117	8	our	our	PRON
biotropia-182	117	9	previous	previous	ADJ
biotropia-182	117	10	study	study	NOUN
biotropia-182	117	11	on	on	ADP
biotropia-182	117	12	e.	e.	PROPN
biotropia-182	117	13	coli	coli	PROPN
biotropia-182	117	14	recombinant	recombinant	ADJ
biotropia-182	117	15	harboring	harboring	NOUN
biotropia-182	117	16	chi	chi	NOUN
biotropia-182	117	17	of	of	ADP
biotropia-182	117	18	ws7b	ws7b	PROPN
biotropia-182	117	19	in	in	ADP
biotropia-182	117	20	a	a	DET
biotropia-182	117	21	puc	puc	NOUN
biotropia-182	117	22	vector	vector	NOUN
biotropia-182	117	23	that	that	PRON
biotropia-182	117	24	ws7b	ws7b	PROPN
biotropia-182	117	25	presumably	presumably	ADV
biotropia-182	117	26	carries	carry	VERB
biotropia-182	117	27	more	more	ADJ
biotropia-182	117	28	than	than	ADP
biotropia-182	117	29	one	one	NUM
biotropia-182	117	30	chitinase	chitinase	NOUN
biotropia-182	117	31	gene	gene	NOUN
biotropia-182	117	32	,	,	PUNCT
biotropia-182	117	33	and	and	CCONJ
biotropia-182	117	34	has	have	VERB
biotropia-182	117	35	a	a	DET
biotropia-182	117	36	complex	complex	ADJ
biotropia-182	117	37	regulation	regulation	NOUN
biotropia-182	117	38	of	of	ADP
biotropia-182	117	39	chitinase	chitinase	NOUN
biotropia-182	117	40	gene	gene	NOUN
biotropia-182	117	41	expression	expression	NOUN
biotropia-182	117	42	.	.	PUNCT
biotropia-182	118	1	table	table	NOUN
biotropia-182	118	2	3	3	NUM
biotropia-182	118	3	.	.	PUNCT
biotropia-182	119	1	chitinase	chitinase	NOUN
biotropia-182	119	2	activity	activity	NOUN
biotropia-182	119	3	assay	assay	NOUN
biotropia-182	119	4	chitinase	chitinase	NOUN
biotropia-182	119	5	assay	assay	PROPN
biotropia-182	119	6	(	(	PUNCT
biotropia-182	119	7	unit	unit	NOUN
biotropia-182	119	8	/	/	SYM
biotropia-182	119	9	mg	mg	PROPN
biotropia-182	119	10	protein	protein	NOUN
biotropia-182	119	11	)	)	PUNCT
biotropia-182	119	12	strains	strain	VERB
biotropia-182	119	13	extracellular	extracellular	ADJ
biotropia-182	119	14	intracellular	intracellular	ADJ
biotropia-182	119	15	a.	a.	NOUN
biotropia-182	119	16	caviae	caviae	NOUN
biotropia-182	119	17	ws7b	ws7b	PROPN
biotropia-182	119	18	7.70	7.70	NUM
biotropia-182	119	19	"	"	PUNCT
biotropia-182	119	20	nd	nd	PRON
biotropia-182	119	21	e.	e.	PROPN
biotropia-182	119	22	co//top10	co//top10	PROPN
biotropia-182	120	1	0.29	0.29	NUM
biotropia-182	120	2	0.41	0.41	NUM
biotropia-182	120	3	e.	e.	PROPN
biotropia-182	120	4	co//top10(pws506	co//top10(pws506	PROPN
biotropia-182	120	5	)	)	PUNCT
biotropia-182	120	6	2.31	2.31	NUM
biotropia-182	120	7	4.17	4.17	NUM
biotropia-182	120	8	eco	eco	PROPN
biotropia-182	120	9	/	/	SYM
biotropia-182	120	10	itop10(pam340	itop10(pam340	PROPN
biotropia-182	120	11	)	)	PUNCT
biotropia-182	120	12	0.93	0.93	NUM
biotropia-182	120	13	1.12	1.12	NUM
biotropia-182	120	14	e.	e.	PROPN
biotropia-182	120	15	co/;top10(pam630	co/;top10(pam630	PROPN
biotropia-182	120	16	)	)	PUNCT
biotropia-182	120	17	0.91	0.91	NUM
biotropia-182	120	18	1.05	1.05	NUM
biotropia-182	120	19	p.	p.	NOUN
biotropia-182	120	20	fluorescens	fluorescen	NOUN
biotropia-182	120	21	5100	5100	NUM
biotropia-182	120	22	rif	rif	PROPN
biotropia-182	120	23	"	"	PUNCT
biotropia-182	120	24	0.77	0.77	NUM
biotropia-182	120	25	0.84	0.84	NUM
biotropia-182	120	26	p.	p.	NOUN
biotropia-182	120	27	fluorescens	fluorescen	NOUN
biotropia-182	120	28	(	(	PUNCT
biotropia-182	120	29	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	120	30	)	)	PUNCT
biotropia-182	120	31	0.78	0.78	NUM
biotropia-182	120	32	0.83	0.83	NUM
biotropia-182	120	33	p.	p.	NOUN
biotropia-182	120	34	fluorescens	fluorescen	NOUN
biotropia-182	120	35	(	(	PUNCT
biotropia-182	120	36	pam340	pam340	NOUN
biotropia-182	120	37	)	)	PUNCT
biotropia-182	120	38	1.91	1.91	NUM
biotropia-182	120	39	3.27	3.27	NUM
biotropia-182	120	40	p.	p.	NOUN
biotropia-182	120	41	fluorescens	fluorescen	NOUN
biotropia-182	120	42	(	(	PUNCT
biotropia-182	120	43	pam630	pam630	PROPN
biotropia-182	120	44	)	)	PUNCT
biotropia-182	120	45	0.97	0.97	NUM
biotropia-182	120	46	2.53	2.53	NUM
biotropia-182	120	47	#	#	NOUN
biotropia-182	120	48	as	as	ADP
biotropia-182	120	49	positive	positive	ADJ
biotropia-182	120	50	control	control	NOUN
biotropia-182	120	51	,	,	PUNCT
biotropia-182	120	52	collected	collect	VERB
biotropia-182	120	53	from90	from90	NOUN
biotropia-182	120	54	hrs	hrs	NOUN
biotropia-182	120	55	of	of	ADP
biotropia-182	120	56	growth	growth	NOUN
biotropia-182	120	57	incubation	incubation	NOUN
biotropia-182	120	58	time	time	NOUN
biotropia-182	120	59	nd	nd	ADP
biotropia-182	120	60	=	=	SYM
biotropia-182	120	61	not	not	PART
biotropia-182	120	62	determined	determine	VERB
biotropia-182	120	63	biotropia	biotropia	ADJ
biotropia-182	120	64	no	no	NOUN
biotropia-182	120	65	.	.	PUNCT
biotropia-182	121	1	20,2003	20,2003	NUM
biotropia-182	121	2	antifungal	antifungal	ADJ
biotropia-182	121	3	plate	plate	NOUN
biotropia-182	121	4	assay	assay	VERB
biotropia-182	121	5	the	the	DET
biotropia-182	121	6	antagonism	antagonism	NOUN
biotropia-182	121	7	assay	assay	NOUN
biotropia-182	121	8	of	of	ADP
biotropia-182	121	9	p.	p.	PROPN
biotropia-182	121	10	fluorescens	fluorescens	PROPN
biotropia-182	121	11	strains	strain	VERB
biotropia-182	121	12	harboring	harbor	VERB
biotropia-182	121	13	pam340	pam340	NOUN
biotropia-182	121	14	,	,	PUNCT
biotropia-182	121	15	and	and	CCONJ
biotropia-182	121	16	pam630	pam630	NOUN
biotropia-182	121	17	,	,	PUNCT
biotropia-182	121	18	was	be	AUX
biotropia-182	121	19	conducted	conduct	VERB
biotropia-182	121	20	with	with	ADP
biotropia-182	121	21	a.	a.	NOUN
biotropia-182	121	22	caviae	caviae	PROPN
biotropia-182	121	23	ws7b	ws7b	PROPN
biotropia-182	121	24	as	as	ADP
biotropia-182	121	25	positive	positive	ADJ
biotropia-182	121	26	control	control	NOUN
biotropia-182	121	27	for	for	ADP
biotropia-182	121	28	chitinolytic	chitinolytic	ADJ
biotropia-182	121	29	activity	activity	NOUN
biotropia-182	121	30	.	.	PUNCT
biotropia-182	122	1	p.	p.	NOUN
biotropia-182	122	2	fluorescens	fluorescen	NOUN
biotropia-182	122	3	5100	5100	NUM
biotropia-182	122	4	rif	rif	PROPN
biotropia-182	122	5	and	and	CCONJ
biotropia-182	122	6	p.	p.	NOUN
biotropia-182	122	7	fluorescens	fluorescen	NOUN
biotropia-182	123	1	(	(	PUNCT
biotropia-182	123	2	pbbr!mcs2	pbbr!mcs2	NOUN
biotropia-182	123	3	)	)	PUNCT
biotropia-182	123	4	were	be	AUX
biotropia-182	123	5	used	use	VERB
biotropia-182	123	6	as	as	ADP
biotropia-182	123	7	negative	negative	ADJ
biotropia-182	123	8	control	control	NOUN
biotropia-182	123	9	strains	strain	VERB
biotropia-182	123	10	.	.	PUNCT
biotropia-182	124	1	the	the	DET
biotropia-182	124	2	inhibition	inhibition	NOUN
biotropia-182	124	3	zones	zone	NOUN
biotropia-182	124	4	between	between	ADP
biotropia-182	124	5	the	the	DET
biotropia-182	124	6	pathogenic	pathogenic	ADJ
biotropia-182	124	7	fungus	fungus	NOUN
biotropia-182	124	8	botrytis	botrytis	NOUN
biotropia-182	124	9	cinerea	cinerea	NOUN
biotropia-182	124	10	tom98ld	tom98ld	NOUN
biotropia-182	124	11	and	and	CCONJ
biotropia-182	124	12	the	the	DET
biotropia-182	124	13	tested	test	VERB
biotropia-182	124	14	strains	strain	NOUN
biotropia-182	124	15	were	be	AUX
biotropia-182	124	16	observed	observe	VERB
biotropia-182	124	17	after	after	ADP
biotropia-182	124	18	3	3	NUM
biotropia-182	124	19	to	to	PART
biotropia-182	124	20	24	24	NUM
biotropia-182	124	21	days	day	NOUN
biotropia-182	124	22	of	of	ADP
biotropia-182	124	23	incubation	incubation	NOUN
biotropia-182	124	24	.	.	PUNCT
biotropia-182	125	1	the	the	DET
biotropia-182	125	2	positive	positive	ADJ
biotropia-182	125	3	control	control	NOUN
biotropia-182	125	4	,	,	PUNCT
biotropia-182	125	5	ws7b	ws7b	PROPN
biotropia-182	125	6	,	,	PUNCT
biotropia-182	125	7	showed	show	VERB
biotropia-182	125	8	an	an	DET
biotropia-182	125	9	inhibition	inhibition	NOUN
biotropia-182	125	10	zone	zone	NOUN
biotropia-182	125	11	up	up	ADP
biotropia-182	125	12	to	to	ADP
biotropia-182	125	13	17	17	NUM
biotropia-182	125	14	mm	mm	NOUN
biotropia-182	125	15	.	.	PUNCT
biotropia-182	126	1	however	however	ADV
biotropia-182	126	2	,	,	PUNCT
biotropia-182	126	3	all	all	DET
biotropia-182	126	4	bacteria	bacteria	NOUN
biotropia-182	126	5	strains	strain	NOUN
biotropia-182	126	6	tested	test	VERB
biotropia-182	126	7	did	do	AUX
biotropia-182	126	8	not	not	PART
biotropia-182	126	9	show	show	VERB
biotropia-182	126	10	any	any	DET
biotropia-182	126	11	fungal	fungal	ADJ
biotropia-182	126	12	growth	growth	NOUN
biotropia-182	126	13	inhibition	inhibition	NOUN
biotropia-182	126	14	against	against	ADP
biotropia-182	126	15	fusarium	fusarium	NOUN
biotropia-182	126	16	solani	solani	PROPN
biotropia-182	126	17	f.	f.	PROPN
biotropia-182	126	18	sp	sp	PROPN
biotropia-182	126	19	.	.	PROPN
biotropia-182	126	20	pisi	pisi	PROPN
biotropia-182	126	21	.	.	PROPN
biotropia-182	126	22	discussion	discussion	NOUN
biotropia-182	126	23	a	a	DET
biotropia-182	126	24	transcriptional	transcriptional	ADJ
biotropia-182	126	25	fusion	fusion	NOUN
biotropia-182	126	26	of	of	ADP
biotropia-182	126	27	a.	a.	NOUN
biotropia-182	126	28	caviae	caviae	PROPN
biotropia-182	126	29	chitinase	chitinase	PROPN
biotropia-182	126	30	gene	gene	NOUN
biotropia-182	126	31	(	(	PUNCT
biotropia-182	126	32	chi	chi	NOUN
biotropia-182	126	33	)	)	PUNCT
biotropia-182	126	34	under	under	ADP
biotropia-182	126	35	kanamycin	kanamycin	PROPN
biotropia-182	126	36	resistance	resistance	NOUN
biotropia-182	126	37	gene	gene	NOUN
biotropia-182	126	38	promoter	promoter	NOUN
biotropia-182	126	39	(	(	PUNCT
biotropia-182	126	40	pkmr	pkmr	NOUN
biotropia-182	126	41	)	)	PUNCT
biotropia-182	126	42	has	have	AUX
biotropia-182	126	43	been	be	AUX
biotropia-182	126	44	constructed	construct	VERB
biotropia-182	126	45	.	.	PUNCT
biotropia-182	127	1	kmr	kmr	PROPN
biotropia-182	127	2	gene	gene	PROPN
biotropia-182	127	3	is	be	AUX
biotropia-182	127	4	known	know	VERB
biotropia-182	127	5	to	to	PART
biotropia-182	127	6	be	be	AUX
biotropia-182	127	7	expressed	express	VERB
biotropia-182	127	8	constitutively	constitutively	ADV
biotropia-182	127	9	.	.	PUNCT
biotropia-182	128	1	by	by	ADP
biotropia-182	128	2	inserting	insert	VERB
biotropia-182	128	3	chi	chi	NOUN
biotropia-182	128	4	under	under	ADP
biotropia-182	128	5	this	this	DET
biotropia-182	128	6	promoter	promoter	NOUN
biotropia-182	128	7	,	,	PUNCT
biotropia-182	128	8	we	we	PRON
biotropia-182	128	9	assume	assume	VERB
biotropia-182	128	10	this	this	PRON
biotropia-182	128	11	will	will	AUX
biotropia-182	128	12	cause	cause	VERB
biotropia-182	128	13	simple	simple	ADJ
biotropia-182	128	14	constitutive	constitutive	ADJ
biotropia-182	128	15	regulation	regulation	NOUN
biotropia-182	128	16	of	of	ADP
biotropia-182	128	17	chi	chi	NOUN
biotropia-182	128	18	.	.	PUNCT
biotropia-182	129	1	the	the	DET
biotropia-182	129	2	expression	expression	NOUN
biotropia-182	129	3	of	of	ADP
biotropia-182	129	4	other	other	ADJ
biotropia-182	129	5	chitinase	chitinase	NOUN
biotropia-182	129	6	genes	gene	NOUN
biotropia-182	129	7	has	have	AUX
biotropia-182	129	8	been	be	AUX
biotropia-182	129	9	reported	report	VERB
biotropia-182	129	10	under	under	ADP
biotropia-182	129	11	nonindigenous	nonindigenous	ADJ
biotropia-182	129	12	promoter	promoter	NOUN
biotropia-182	129	13	,	,	PUNCT
biotropia-182	129	14	ptac	ptac	PROPN
biotropia-182	129	15	(	(	PUNCT
biotropia-182	129	16	koby	koby	PROPN
biotropia-182	129	17	et	et	PROPN
biotropia-182	129	18	al	al	PROPN
biotropia-182	129	19	.	.	PROPN
biotropia-182	129	20	1994	1994	NUM
biotropia-182	129	21	;	;	PUNCT
biotropia-182	129	22	downing	downing	NOUN
biotropia-182	129	23	and	and	CCONJ
biotropia-182	129	24	thomson	thomson	NOUN
biotropia-182	129	25	2000	2000	NUM
biotropia-182	129	26	)	)	PUNCT
biotropia-182	129	27	.	.	PUNCT
biotropia-182	130	1	however	however	ADV
biotropia-182	130	2	,	,	PUNCT
biotropia-182	130	3	the	the	DET
biotropia-182	130	4	expression	expression	NOUN
biotropia-182	130	5	of	of	ADP
biotropia-182	130	6	chitinase	chitinase	NOUN
biotropia-182	130	7	gene	gene	NOUN
biotropia-182	130	8	under	under	ADP
biotropia-182	130	9	the	the	DET
biotropia-182	130	10	control	control	NOUN
biotropia-182	130	11	of	of	ADP
biotropia-182	130	12	pkmr	pkmr	NOUN
biotropia-182	130	13	has	have	AUX
biotropia-182	130	14	previously	previously	ADV
biotropia-182	130	15	not	not	PART
biotropia-182	130	16	been	be	AUX
biotropia-182	130	17	reported	report	VERB
biotropia-182	130	18	.	.	PUNCT
biotropia-182	131	1	in	in	ADP
biotropia-182	131	2	this	this	DET
biotropia-182	131	3	study	study	NOUN
biotropia-182	131	4	,	,	PUNCT
biotropia-182	131	5	we	we	PRON
biotropia-182	131	6	demonstrated	demonstrate	VERB
biotropia-182	131	7	the	the	DET
biotropia-182	131	8	constitutive	constitutive	ADJ
biotropia-182	131	9	expression	expression	NOUN
biotropia-182	131	10	of	of	ADP
biotropia-182	131	11	chitinase	chitinase	NOUN
biotropia-182	131	12	under	under	ADP
biotropia-182	131	13	this	this	DET
biotropia-182	131	14	promoter	promoter	NOUN
biotropia-182	131	15	by	by	ADP
biotropia-182	131	16	a	a	DET
biotropia-182	131	17	simple	simple	ADJ
biotropia-182	131	18	semi	semi	ADJ
biotropia-182	131	19	micro	micro	ADJ
biotropia-182	131	20	-	-	ADJ
biotropia-182	131	21	quantitative	quantitative	ADJ
biotropia-182	131	22	chromogenic	chromogenic	ADJ
biotropia-182	131	23	assay	assay	NOUN
biotropia-182	131	24	using	use	VERB
biotropia-182	131	25	colloidal	colloidal	NOUN
biotropia-182	131	26	chitin	chitin	NOUN
biotropia-182	131	27	azure	azure	NOUN
biotropia-182	131	28	as	as	ADP
biotropia-182	131	29	substrate	substrate	NOUN
biotropia-182	131	30	.	.	PUNCT
biotropia-182	132	1	the	the	DET
biotropia-182	132	2	enzyme	enzyme	NOUN
biotropia-182	132	3	activity	activity	NOUN
biotropia-182	132	4	was	be	AUX
biotropia-182	132	5	determined	determine	VERB
biotropia-182	132	6	by	by	ADP
biotropia-182	132	7	measuring	measure	VERB
biotropia-182	132	8	the	the	DET
biotropia-182	132	9	amount	amount	NOUN
biotropia-182	132	10	of	of	ADP
biotropia-182	132	11	remazol	remazol	NOUN
biotropia-182	132	12	brilliant	brilliant	ADJ
biotropia-182	132	13	blue	blue	ADJ
biotropia-182	132	14	dye	dye	NOUN
biotropia-182	132	15	released	release	VERB
biotropia-182	132	16	employing	employ	VERB
biotropia-182	132	17	spectrophotometer	spectrophotometer	NOUN
biotropia-182	132	18	.	.	PUNCT
biotropia-182	133	1	the	the	DET
biotropia-182	133	2	results	result	NOUN
biotropia-182	133	3	from	from	ADP
biotropia-182	133	4	the	the	DET
biotropia-182	133	5	expression	expression	NOUN
biotropia-182	133	6	of	of	ADP
biotropia-182	133	7	chitinase	chitinase	NOUN
biotropia-182	133	8	transcriptional	transcriptional	ADJ
biotropia-182	133	9	fusion	fusion	NOUN
biotropia-182	133	10	assay	assay	NOUN
biotropia-182	133	11	demonstrated	demonstrate	VERB
biotropia-182	133	12	that	that	SCONJ
biotropia-182	133	13	both	both	DET
biotropia-182	133	14	transcriptional	transcriptional	ADJ
biotropia-182	133	15	fusions	fusion	NOUN
biotropia-182	133	16	were	be	AUX
biotropia-182	133	17	expressed	express	VERB
biotropia-182	133	18	in	in	ADP
biotropia-182	133	19	e.	e.	PROPN
biotropia-182	133	20	coli	coli	PROPN
biotropia-182	133	21	,	,	PUNCT
biotropia-182	133	22	and	and	CCONJ
biotropia-182	133	23	were	be	AUX
biotropia-182	133	24	able	able	ADJ
biotropia-182	133	25	to	to	PART
biotropia-182	133	26	degrade	degrade	VERB
biotropia-182	133	27	chitin	chitin	NOUN
biotropia-182	133	28	in	in	ADP
biotropia-182	133	29	the	the	DET
biotropia-182	133	30	medium	medium	NOUN
biotropia-182	133	31	.	.	PUNCT
biotropia-182	134	1	longer	long	ADJ
biotropia-182	134	2	incubation	incubation	NOUN
biotropia-182	134	3	time	time	NOUN
biotropia-182	134	4	for	for	ADP
biotropia-182	134	5	chitinolytic	chitinolytic	ADJ
biotropia-182	134	6	activity	activity	NOUN
biotropia-182	134	7	of	of	ADP
biotropia-182	134	8	pam340	pam340	NOUN
biotropia-182	134	9	and	and	CCONJ
biotropia-182	134	10	pam630	pam630	NOUN
biotropia-182	134	11	compared	compare	VERB
biotropia-182	134	12	to	to	ADP
biotropia-182	134	13	pws506	pws506	PROPN
biotropia-182	134	14	,	,	PUNCT
biotropia-182	134	15	might	might	AUX
biotropia-182	134	16	be	be	AUX
biotropia-182	134	17	due	due	ADJ
biotropia-182	134	18	to	to	ADP
biotropia-182	134	19	the	the	DET
biotropia-182	134	20	influence	influence	NOUN
biotropia-182	134	21	of	of	ADP
biotropia-182	134	22	plasmid	plasmid	NOUN
biotropia-182	134	23	copy	copy	NOUN
biotropia-182	134	24	number	number	NOUN
biotropia-182	134	25	in	in	ADP
biotropia-182	134	26	this	this	DET
biotropia-182	134	27	expression	expression	NOUN
biotropia-182	134	28	(	(	PUNCT
biotropia-182	134	29	table	table	NOUN
biotropia-182	134	30	1	1	NUM
biotropia-182	134	31	)	)	PUNCT
biotropia-182	134	32	.	.	PUNCT
biotropia-182	135	1	plasmid	plasmid	ADJ
biotropia-182	135	2	pam340	pam340	PROPN
biotropia-182	135	3	and	and	CCONJ
biotropia-182	135	4	pam630	pam630	PROPN
biotropia-182	135	5	were	be	AUX
biotropia-182	135	6	constructed	construct	VERB
biotropia-182	135	7	on	on	ADP
biotropia-182	135	8	a	a	DET
biotropia-182	135	9	medium	medium	ADJ
biotropia-182	135	10	copy	copy	NOUN
biotropia-182	135	11	number	number	NOUN
biotropia-182	135	12	plasmid	plasmid	VERB
biotropia-182	135	13	vector	vector	NOUN
biotropia-182	135	14	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	135	15	,	,	PUNCT
biotropia-182	135	16	while	while	SCONJ
biotropia-182	135	17	pws506	pws506	PROPN
biotropia-182	135	18	was	be	AUX
biotropia-182	135	19	constructed	construct	VERB
biotropia-182	135	20	on	on	ADP
biotropia-182	135	21	a	a	DET
biotropia-182	135	22	high	high	ADJ
biotropia-182	135	23	copy	copy	NOUN
biotropia-182	135	24	number	number	NOUN
biotropia-182	135	25	plasmid	plasmid	NOUN
biotropia-182	135	26	vector	vector	NOUN
biotropia-182	135	27	pucl	pucl	NOUN
biotropia-182	135	28	9	9	NUM
biotropia-182	135	29	.	.	PUNCT
biotropia-182	136	1	the	the	DET
biotropia-182	136	2	strategy	strategy	NOUN
biotropia-182	136	3	to	to	PART
biotropia-182	136	4	isolate	isolate	VERB
biotropia-182	136	5	and	and	CCONJ
biotropia-182	136	6	insert	insert	VERB
biotropia-182	136	7	the	the	DET
biotropia-182	136	8	promoters	promoter	NOUN
biotropia-182	136	9	into	into	ADP
biotropia-182	136	10	plasmid	plasmid	ADJ
biotropia-182	136	11	vector	vector	NOUN
biotropia-182	136	12	pbbrlmcs-2	pbbrlmcs-2	NUM
biotropia-182	136	13	before	before	ADP
biotropia-182	136	14	fusion	fusion	NOUN
biotropia-182	136	15	with	with	ADP
biotropia-182	136	16	chi	chi	NOUN
biotropia-182	136	17	,	,	PUNCT
biotropia-182	136	18	rather	rather	ADV
biotropia-182	136	19	then	then	ADV
biotropia-182	136	20	subcloned	subclone	VERB
biotropia-182	136	21	chi	chi	NOUN
biotropia-182	136	22	under	under	ADP
biotropia-182	136	23	pkmr	pkmr	NOUN
biotropia-182	136	24	directly	directly	ADV
biotropia-182	136	25	in	in	ADP
biotropia-182	136	26	puc4k	puc4k	PROPN
biotropia-182	136	27	,	,	PUNCT
biotropia-182	136	28	as	as	ADV
biotropia-182	136	29	well	well	ADV
biotropia-182	136	30	as	as	ADP
biotropia-182	136	31	under	under	ADP
biotropia-182	136	32	ptac	ptac	NOUN
biotropia-182	136	33	in	in	ADP
biotropia-182	136	34	pkk223	pkk223	PROPN
biotropia-182	136	35	-	-	PUNCT
biotropia-182	136	36	3	3	NUM
biotropia-182	136	37	,	,	PUNCT
biotropia-182	136	38	was	be	AUX
biotropia-182	136	39	carried	carry	VERB
biotropia-182	136	40	out	out	ADP
biotropia-182	136	41	to	to	PART
biotropia-182	136	42	yield	yield	VERB
biotropia-182	136	43	a	a	DET
biotropia-182	136	44	broad	broad	ADJ
biotropia-182	136	45	host	host	NOUN
biotropia-182	136	46	-	-	PUNCT
biotropia-182	136	47	range	range	NOUN
biotropia-182	136	48	plasmid	plasmid	ADJ
biotropia-182	136	49	vector	vector	NOUN
biotropia-182	136	50	that	that	PRON
biotropia-182	136	51	carry	carry	VERB
biotropia-182	136	52	a	a	DET
biotropia-182	136	53	constitutive	constitutive	ADJ
biotropia-182	136	54	promoter	promoter	NOUN
biotropia-182	136	55	pkmr	pkmr	NOUN
biotropia-182	136	56	,	,	PUNCT
biotropia-182	136	57	as	as	ADV
biotropia-182	136	58	well	well	ADV
biotropia-182	136	59	as	as	ADP
biotropia-182	136	60	the	the	DET
biotropia-182	136	61	strong	strong	ADJ
biotropia-182	136	62	promoter	promoter	NOUN
biotropia-182	136	63	ptac	ptac	NOUN
biotropia-182	136	64	,	,	PUNCT
biotropia-182	136	65	which	which	PRON
biotropia-182	136	66	will	will	AUX
biotropia-182	136	67	be	be	AUX
biotropia-182	136	68	useful	useful	ADJ
biotropia-182	136	69	in	in	ADP
biotropia-182	136	70	future	future	ADJ
biotropia-182	136	71	studies	study	NOUN
biotropia-182	136	72	of	of	ADP
biotropia-182	136	73	other	other	ADJ
biotropia-182	136	74	heterologous	heterologous	ADJ
biotropia-182	136	75	gene	gene	NOUN
biotropia-182	136	76	expressions	expression	NOUN
biotropia-182	136	77	.	.	PUNCT
biotropia-182	137	1	the	the	DET
biotropia-182	137	2	result	result	NOUN
biotropia-182	137	3	of	of	ADP
biotropia-182	137	4	the	the	DET
biotropia-182	137	5	semimicro	semimicro	NOUN
biotropia-182	137	6	-	-	PROPN
biotropia-182	137	7	quantitative	quantitative	ADJ
biotropia-182	137	8	of	of	ADP
biotropia-182	137	9	chitinase	chitinase	NOUN
biotropia-182	137	10	activity	activity	NOUN
biotropia-182	137	11	assay	assay	NOUN
biotropia-182	137	12	indicated	indicate	VERB
biotropia-182	137	13	that	that	SCONJ
biotropia-182	137	14	chi	chi	PROPN
biotropia-182	137	15	was	be	AUX
biotropia-182	137	16	expressed	express	VERB
biotropia-182	137	17	constitutively	constitutively	ADV
biotropia-182	137	18	both	both	CCONJ
biotropia-182	137	19	in	in	ADP
biotropia-182	137	20	e.	e.	PROPN
biotropia-182	137	21	coli	coli	PROPN
biotropia-182	137	22	or	or	CCONJ
biotropia-182	137	23	p.	p.	NOUN
biotropia-182	137	24	fluorescens	fluorescen	NOUN
biotropia-182	137	25	5100	5100	NUM
biotropia-182	137	26	recombinants	recombinant	NOUN
biotropia-182	137	27	(	(	PUNCT
biotropia-182	137	28	table	table	NOUN
biotropia-182	137	29	3	3	NUM
biotropia-182	137	30	)	)	PUNCT
biotropia-182	137	31	.	.	PUNCT
biotropia-182	138	1	however	however	ADV
biotropia-182	138	2	,	,	PUNCT
biotropia-182	138	3	chitinase	chitinase	NOUN
biotropia-182	138	4	activity	activity	NOUN
biotropia-182	138	5	of	of	ADP
biotropia-182	138	6	intracellular	intracellular	ADJ
biotropia-182	138	7	fractions	fraction	NOUN
biotropia-182	138	8	were	be	AUX
biotropia-182	138	9	higher	high	ADJ
biotropia-182	138	10	than	than	ADP
biotropia-182	138	11	the	the	DET
biotropia-182	138	12	extracellular	extracellular	ADJ
biotropia-182	138	13	fractions	fraction	NOUN
biotropia-182	138	14	which	which	PRON
biotropia-182	138	15	can	can	AUX
biotropia-182	138	16	be	be	AUX
biotropia-182	138	17	assumed	assume	VERB
biotropia-182	138	18	that	that	SCONJ
biotropia-182	138	19	the	the	DET
biotropia-182	138	20	chitinase	chitinase	NOUN
biotropia-182	138	21	product	product	NOUN
biotropia-182	138	22	might	might	AUX
biotropia-182	138	23	not	not	PART
biotropia-182	138	24	be	be	AUX
biotropia-182	138	25	well	well	ADV
biotropia-182	138	26	secreted	secrete	VERB
biotropia-182	138	27	in	in	ADP
biotropia-182	138	28	e.	e.	PROPN
biotropia-182	138	29	coli	coli	PROPN
biotropia-182	138	30	,	,	PUNCT
biotropia-182	138	31	as	as	SCONJ
biotropia-182	138	32	we	we	PRON
biotropia-182	138	33	have	have	AUX
biotropia-182	138	34	discussed	discuss	VERB
biotropia-182	138	35	in	in	ADP
biotropia-182	138	36	our	our	PRON
biotropia-182	138	37	previous	previous	ADJ
biotropia-182	138	38	study	study	NOUN
biotropia-182	138	39	,	,	PUNCT
biotropia-182	138	40	as	as	ADV
biotropia-182	138	41	well	well	ADV
biotropia-182	138	42	as	as	ADP
biotropia-182	138	43	in	in	ADP
biotropia-182	138	44	p.	p.	NOUN
biotropia-182	138	45	fluorescens	fluorescen	NOUN
biotropia-182	138	46	(	(	PUNCT
biotropia-182	138	47	malik	malik	PROPN
biotropia-182	138	48	et	et	PROPN
biotropia-182	138	49	al	al	PROPN
biotropia-182	138	50	.	.	PROPN
biotropia-182	139	1	in	in	ADP
biotropia-182	139	2	press	press	NOUN
biotropia-182	139	3	)	)	PUNCT
biotropia-182	139	4	.	.	PUNCT
biotropia-182	140	1	45	45	NUM
biotropia-182	140	2	heterologous	heterologous	ADJ
biotropia-182	140	3	expression	expression	NOUN
biotropia-182	140	4	of	of	ADP
biotropia-182	140	5	a	a	DET
biotropia-182	140	6	chitinase	chitinase	NOUN
biotropia-182	140	7	gene	gene	NOUN
biotropia-182	140	8	from	from	ADP
biotropia-182	140	9	aeromonas	aeromonas	PROPN
biotropia-182	140	10	caviae	caviae	PROPN
biotropia-182	140	11	amarila	amarila	PROPN
biotropia-182	140	12	malik	malik	PROPN
biotropia-182	140	13	et	et	PROPN
biotropia-182	140	14	al	al	PROPN
biotropia-182	140	15	.	.	PUNCT
biotropia-182	141	1	the	the	DET
biotropia-182	141	2	inability	inability	NOUN
biotropia-182	141	3	of	of	ADP
biotropia-182	141	4	transformed	transform	VERB
biotropia-182	141	5	p.	p.	NOUN
biotropia-182	141	6	fluorescens	fluorescen	NOUN
biotropia-182	141	7	strains	strain	VERB
biotropia-182	141	8	to	to	PART
biotropia-182	141	9	slow	slow	VERB
biotropia-182	141	10	fungal	fungal	ADJ
biotropia-182	141	11	growth	growth	NOUN
biotropia-182	141	12	is	be	AUX
biotropia-182	141	13	more	more	ADV
biotropia-182	141	14	likely	likely	ADV
biotropia-182	141	15	caused	cause	VERB
biotropia-182	141	16	by	by	ADP
biotropia-182	141	17	secretion	secretion	NOUN
biotropia-182	141	18	problem	problem	NOUN
biotropia-182	141	19	,	,	PUNCT
biotropia-182	141	20	as	as	ADV
biotropia-182	141	21	well	well	ADV
biotropia-182	141	22	as	as	ADP
biotropia-182	141	23	low	low	ADJ
biotropia-182	141	24	expression	expression	NOUN
biotropia-182	141	25	of	of	ADP
biotropia-182	141	26	chitinase	chitinase	NOUN
biotropia-182	141	27	product	product	NOUN
biotropia-182	141	28	in	in	ADP
biotropia-182	141	29	p.	p.	PROPN
biotropia-182	141	30	fluorescens	fluorescen	NOUN
biotropia-182	142	1	based	base	VERB
biotropia-182	142	2	on	on	ADP
biotropia-182	142	3	the	the	DET
biotropia-182	142	4	results	result	NOUN
biotropia-182	142	5	obtained	obtain	VERB
biotropia-182	142	6	from	from	ADP
biotropia-182	142	7	chitinolytic	chitinolytic	ADJ
biotropia-182	142	8	expression	expression	NOUN
biotropia-182	142	9	on	on	ADP
biotropia-182	142	10	agar	agar	NOUN
biotropia-182	142	11	plates	plate	NOUN
biotropia-182	142	12	and	and	CCONJ
biotropia-182	142	13	semiquantitative	semiquantitative	ADJ
biotropia-182	142	14	chitinase	chitinase	NOUN
biotropia-182	142	15	assays	assay	NOUN
biotropia-182	142	16	as	as	SCONJ
biotropia-182	142	17	shown	show	VERB
biotropia-182	142	18	in	in	ADP
biotropia-182	142	19	tables	table	NOUN
biotropia-182	142	20	2	2	NUM
biotropia-182	142	21	and	and	CCONJ
biotropia-182	142	22	3	3	NUM
biotropia-182	142	23	.	.	PUNCT
biotropia-182	143	1	the	the	DET
biotropia-182	143	2	gene	gene	NOUN
biotropia-182	143	3	was	be	AUX
biotropia-182	143	4	isolated	isolate	VERB
biotropia-182	143	5	from	from	ADP
biotropia-182	143	6	a.	a.	NOUN
biotropia-182	143	7	caviae	caviae	PROPN
biotropia-182	143	8	,	,	PUNCT
biotropia-182	143	9	and	and	CCONJ
biotropia-182	143	10	was	be	AUX
biotropia-182	143	11	cloned	clone	VERB
biotropia-182	143	12	and	and	CCONJ
biotropia-182	143	13	expressed	express	VERB
biotropia-182	143	14	in	in	ADP
biotropia-182	143	15	e.	e.	PROPN
biotropia-182	143	16	coli	coli	PROPN
biotropia-182	143	17	and	and	CCONJ
biotropia-182	143	18	p.	p.	NOUN
biotropia-182	143	19	fluorescens	fluorescen	NOUN
biotropia-182	143	20	,	,	PUNCT
biotropia-182	143	21	which	which	PRON
biotropia-182	143	22	are	be	AUX
biotropia-182	143	23	not	not	PART
biotropia-182	143	24	indigenous	indigenous	ADJ
biotropia-182	143	25	hosts	host	NOUN
biotropia-182	143	26	.	.	PUNCT
biotropia-182	144	1	nevertheless	nevertheless	ADV
biotropia-182	144	2	,	,	PUNCT
biotropia-182	144	3	the	the	DET
biotropia-182	144	4	fact	fact	NOUN
biotropia-182	144	5	that	that	SCONJ
biotropia-182	144	6	the	the	DET
biotropia-182	144	7	protein	protein	NOUN
biotropia-182	144	8	expression	expression	NOUN
biotropia-182	144	9	machinery	machinery	NOUN
biotropia-182	144	10	between	between	ADP
biotropia-182	144	11	these	these	DET
biotropia-182	144	12	three	three	NUM
biotropia-182	144	13	microorganisms	microorganism	NOUN
biotropia-182	144	14	are	be	AUX
biotropia-182	144	15	not	not	PART
biotropia-182	144	16	the	the	DET
biotropia-182	144	17	same	same	ADJ
biotropia-182	144	18	,	,	PUNCT
biotropia-182	144	19	could	could	AUX
biotropia-182	144	20	explain	explain	VERB
biotropia-182	144	21	this	this	DET
biotropia-182	144	22	low	low	ADJ
biotropia-182	144	23	chitinolytic	chitinolytic	ADJ
biotropia-182	144	24	activity	activity	NOUN
biotropia-182	144	25	.	.	PUNCT
biotropia-182	145	1	it	it	PRON
biotropia-182	145	2	could	could	AUX
biotropia-182	145	3	be	be	AUX
biotropia-182	145	4	also	also	ADV
biotropia-182	145	5	suggested	suggest	VERB
biotropia-182	145	6	that	that	SCONJ
biotropia-182	145	7	this	this	DET
biotropia-182	145	8	chitinase	chitinase	NOUN
biotropia-182	145	9	gene	gene	NOUN
biotropia-182	145	10	(	(	PUNCT
biotropia-182	145	11	chi	chi	NOUN
biotropia-182	145	12	)	)	PUNCT
biotropia-182	145	13	in	in	ADP
biotropia-182	145	14	its	its	PRON
biotropia-182	145	15	source	source	NOUN
biotropia-182	145	16	bacteria	bacteria	NOUN
biotropia-182	145	17	a.	a.	NOUN
biotropia-182	145	18	caviae	caviae	PROPN
biotropia-182	145	19	ws7b	ws7b	PROPN
biotropia-182	145	20	,	,	PUNCT
biotropia-182	145	21	did	do	AUX
biotropia-182	145	22	not	not	PART
biotropia-182	145	23	act	act	VERB
biotropia-182	145	24	alone	alone	ADV
biotropia-182	145	25	to	to	PART
biotropia-182	145	26	slow	slow	VERB
biotropia-182	145	27	fungal	fungal	ADJ
biotropia-182	145	28	growth	growth	NOUN
biotropia-182	145	29	.	.	PUNCT
biotropia-182	146	1	it	it	PRON
biotropia-182	146	2	might	might	AUX
biotropia-182	146	3	also	also	ADV
biotropia-182	146	4	require	require	VERB
biotropia-182	146	5	other	other	ADJ
biotropia-182	146	6	antifungal	antifungal	ADJ
biotropia-182	146	7	mechanisms	mechanism	NOUN
biotropia-182	146	8	as	as	SCONJ
biotropia-182	146	9	reported	report	VERB
biotropia-182	146	10	previously	previously	ADV
biotropia-182	146	11	in	in	ADP
biotropia-182	146	12	jones	jones	PROPN
biotropia-182	146	13	et	et	PROPN
biotropia-182	146	14	al	al	PROPN
biotropia-182	146	15	.	.	PROPN
biotropia-182	147	1	(	(	PUNCT
biotropia-182	147	2	1986	1986	NUM
biotropia-182	147	3	)	)	PUNCT
biotropia-182	147	4	and	and	CCONJ
biotropia-182	147	5	sundheim	sundheim	PROPN
biotropia-182	147	6	et	et	PROPN
biotropia-182	147	7	al	al	PROPN
biotropia-182	147	8	.	.	PROPN
biotropia-182	148	1	(	(	PUNCT
biotropia-182	148	2	1988	1988	NUM
biotropia-182	148	3	)	)	PUNCT
biotropia-182	148	4	.	.	PUNCT
biotropia-182	149	1	conclusions	conclusion	NOUN
biotropia-182	149	2	transcriptional	transcriptional	ADJ
biotropia-182	149	3	fusion	fusion	NOUN
biotropia-182	149	4	of	of	ADP
biotropia-182	149	5	the	the	DET
biotropia-182	149	6	chi	chi	ADJ
biotropia-182	149	7	gene	gene	NOUN
biotropia-182	149	8	under	under	ADP
biotropia-182	149	9	the	the	DET
biotropia-182	149	10	control	control	NOUN
biotropia-182	149	11	of	of	ADP
biotropia-182	149	12	kanamycin	kanamycin	PROPN
biotropia-182	149	13	resistance	resistance	NOUN
biotropia-182	149	14	gene	gene	NOUN
biotropia-182	149	15	promoter	promoter	NOUN
biotropia-182	149	16	generated	generate	VERB
biotropia-182	149	17	constitutive	constitutive	ADJ
biotropia-182	149	18	expression	expression	NOUN
biotropia-182	149	19	of	of	ADP
biotropia-182	149	20	chitinase	chitinase	NOUN
biotropia-182	149	21	in	in	ADP
biotropia-182	149	22	both	both	DET
biotropia-182	149	23	e.	e.	PROPN
biotropia-182	149	24	coli	coli	PROPN
biotropia-182	149	25	and	and	CCONJ
biotropia-182	149	26	p.	p.	NOUN
biotropia-182	149	27	fluorescens	fluorescen	NOUN
biotropia-182	149	28	5100	5100	NUM
biotropia-182	149	29	,	,	PUNCT
biotropia-182	149	30	which	which	PRON
biotropia-182	149	31	has	have	AUX
biotropia-182	149	32	previously	previously	ADV
biotropia-182	149	33	not	not	PART
biotropia-182	149	34	been	be	AUX
biotropia-182	149	35	reported	report	VERB
biotropia-182	149	36	.	.	PUNCT
biotropia-182	150	1	this	this	DET
biotropia-182	150	2	chitinase	chitinase	NOUN
biotropia-182	150	3	gene	gene	NOUN
biotropia-182	150	4	expression	expression	NOUN
biotropia-182	150	5	might	might	AUX
biotropia-182	150	6	be	be	AUX
biotropia-182	150	7	useful	useful	ADJ
biotropia-182	150	8	to	to	PART
biotropia-182	150	9	be	be	AUX
biotropia-182	150	10	developed	develop	VERB
biotropia-182	150	11	further	far	ADV
biotropia-182	150	12	for	for	ADP
biotropia-182	150	13	construction	construction	NOUN
biotropia-182	150	14	of	of	ADP
biotropia-182	150	15	a	a	DET
biotropia-182	150	16	biocontrol	biocontrol	NOUN
biotropia-182	150	17	strain	strain	NOUN
biotropia-182	150	18	to	to	PART
biotropia-182	150	19	prevent	prevent	VERB
biotropia-182	150	20	the	the	DET
biotropia-182	150	21	growth	growth	NOUN
biotropia-182	150	22	of	of	ADP
biotropia-182	150	23	phytopathogenic	phytopathogenic	ADJ
biotropia-182	150	24	fungi	fungi	PROPN
biotropia-182	150	25	.	.	PUNCT
biotropia-182	151	1	acknowledgment	acknowledgment	NOUN
biotropia-182	151	2	this	this	DET
biotropia-182	151	3	work	work	NOUN
biotropia-182	151	4	was	be	AUX
biotropia-182	151	5	supported	support	VERB
biotropia-182	151	6	by	by	ADP
biotropia-182	151	7	graduate	graduate	NOUN
biotropia-182	151	8	team	team	NOUN
biotropia-182	151	9	research	research	NOUN
biotropia-182	151	10	grant	grant	NOUN
biotropia-182	151	11	(	(	PUNCT
biotropia-182	151	12	urge	urge	NOUN
biotropia-182	151	13	)	)	PUNCT
biotropia-182	151	14	grant	grant	NOUN
biotropia-182	151	15	029	029	NUM
biotropia-182	151	16	/	/	SYM
biotropia-182	151	17	htpp	htpp	NOUN
biotropia-182	151	18	/	/	SYM
biotropia-182	151	19	ii/	ii/	NOUN
biotropia-182	151	20	urge/96	urge/96	ADJ
biotropia-182	151	21	to	to	PART
biotropia-182	151	22	b.t	b.t	PROPN
biotropia-182	151	23	.	.	PROPN
biotropia-182	151	24	,	,	PUNCT
biotropia-182	151	25	and	and	CCONJ
biotropia-182	151	26	a	a	DET
biotropia-182	151	27	higher	high	ADJ
biotropia-182	151	28	education	education	NOUN
biotropia-182	151	29	link	link	NOUN
biotropia-182	151	30	award	award	NOUN
biotropia-182	151	31	from	from	ADP
biotropia-182	151	32	the	the	DET
biotropia-182	151	33	department	department	NOUN
biotropia-182	151	34	for	for	ADP
biotropia-182	151	35	international	international	ADJ
biotropia-182	151	36	development	development	NOUN
biotropia-182	151	37	(	(	PUNCT
biotropia-182	151	38	dfid)/british	dfid)/british	ADJ
biotropia-182	151	39	council	council	NOUN
biotropia-182	151	40	to	to	ADP
biotropia-182	151	41	r.h	r.h	PROPN
biotropia-182	151	42	.	.	PROPN
biotropia-182	151	43	and	and	CCONJ
biotropia-182	151	44	a.s	a.s	PROPN
biotropia-182	151	45	.	.	PROPN
biotropia-182	151	46	references	reference	NOUN
biotropia-182	151	47	boiler	boiler	NOUN
biotropia-182	151	48	,	,	PUNCT
biotropia-182	151	49	t.	t.	PROPN
biotropia-182	151	50	,	,	PUNCT
biotropia-182	151	51	gehri	gehri	PROPN
biotropia-182	151	52	,	,	PUNCT
biotropia-182	151	53	a.	a.	PROPN
biotropia-182	151	54	,	,	PUNCT
biotropia-182	151	55	mauch	mauch	PROPN
biotropia-182	151	56	,	,	PUNCT
biotropia-182	151	57	f.	f.	PROPN
biotropia-182	151	58	and	and	CCONJ
biotropia-182	151	59	u.	u.	PROPN
biotropia-182	151	60	vogeli	vogeli	PROPN
biotropia-182	151	61	.	.	PUNCT
biotropia-182	152	1	1983	1983	NUM
biotropia-182	152	2	.	.	PUNCT
biotropia-182	153	1	chitinase	chitinase	NOUN
biotropia-182	153	2	in	in	ADP
biotropia-182	153	3	bean	bean	NOUN
biotropia-182	153	4	leaves	leave	NOUN
biotropia-182	153	5	:	:	PUNCT
biotropia-182	153	6	induction	induction	NOUN
biotropia-182	153	7	by	by	ADP
biotropia-182	153	8	ethylene	ethylene	NOUN
biotropia-182	153	9	,	,	PUNCT
biotropia-182	153	10	purification	purification	NOUN
biotropia-182	153	11	,	,	PUNCT
biotropia-182	153	12	properties	property	NOUN
biotropia-182	153	13	,	,	PUNCT
biotropia-182	153	14	and	and	CCONJ
biotropia-182	153	15	possible	possible	ADJ
biotropia-182	153	16	function	function	NOUN
biotropia-182	153	17	.	.	PUNCT
biotropia-182	154	1	planta	planta	PROPN
biotropia-182	154	2	157,22	157,22	NUM
biotropia-182	154	3	-	-	SYM
biotropia-182	154	4	31	31	NUM
biotropia-182	154	5	.	.	PUNCT
biotropia-182	155	1	bradford	bradford	PROPN
biotropia-182	155	2	,	,	PUNCT
biotropia-182	155	3	m.m	m.m	PROPN
biotropia-182	155	4	.	.	PROPN
biotropia-182	155	5	1976	1976	NUM
biotropia-182	155	6	.	.	PUNCT
biotropia-182	156	1	a	a	DET
biotropia-182	156	2	rapid	rapid	ADJ
biotropia-182	156	3	and	and	CCONJ
biotropia-182	156	4	sensitive	sensitive	ADJ
biotropia-182	156	5	method	method	NOUN
biotropia-182	156	6	for	for	ADP
biotropia-182	156	7	the	the	DET
biotropia-182	156	8	quantification	quantification	NOUN
biotropia-182	156	9	of	of	ADP
biotropia-182	156	10	microgram	microgram	ADJ
biotropia-182	156	11	quantities	quantity	NOUN
biotropia-182	156	12	of	of	ADP
biotropia-182	156	13	protein	protein	NOUN
biotropia-182	156	14	utilizing	utilize	VERB
biotropia-182	156	15	the	the	DET
biotropia-182	156	16	principle	principle	NOUN
biotropia-182	156	17	of	of	ADP
biotropia-182	156	18	protein	protein	NOUN
biotropia-182	156	19	-	-	PUNCT
biotropia-182	156	20	dye	dye	NOUN
biotropia-182	156	21	binding	binding	NOUN
biotropia-182	156	22	.	.	PUNCT
biotropia-182	157	1	anal	anal	PROPN
biotropia-182	157	2	.	.	PUNCT
biotropia-182	157	3	biochem	biochem	PROPN
biotropia-182	157	4	.	.	PUNCT
biotropia-182	158	1	72,248	72,248	NUM
biotropia-182	158	2	-	-	SYM
biotropia-182	158	3	254	254	NUM
biotropia-182	158	4	.	.	PUNCT
biotropia-182	159	1	campbell	campbell	PROPN
biotropia-182	159	2	,	,	PUNCT
biotropia-182	159	3	l.j	l.j	PROPN
biotropia-182	159	4	.	.	PROPN
biotropia-182	159	5	,	,	PUNCT
biotropia-182	159	6	marling	marling	PROPN
biotropia-182	159	7	,	,	PUNCT
biotropia-182	159	8	r.	r.	PROPN
biotropia-182	159	9	,	,	PUNCT
biotropia-182	159	10	chard	chard	NOUN
biotropia-182	159	11	,	,	PUNCT
biotropia-182	159	12	j.	j.	PROPN
biotropia-182	159	13	and	and	CCONJ
biotropia-182	159	14	m.	m.	PROPN
biotropia-182	159	15	sutton	sutton	PROPN
biotropia-182	159	16	.	.	PUNCT
biotropia-182	160	1	1995	1995	NUM
biotropia-182	160	2	.	.	PUNCT
biotropia-182	161	1	calabrese	calabrese	NOUN
biotropia-182	161	2	:	:	PUNCT
biotropia-182	161	3	factors	factor	NOUN
biotropia-182	161	4	controlling	control	VERB
biotropia-182	161	5	symptom	symptom	NOUN
biotropia-182	161	6	development	development	NOUN
biotropia-182	161	7	in	in	ADP
biotropia-182	161	8	bacterial	bacterial	ADJ
biotropia-182	161	9	spear	spear	NOUN
biotropia-182	161	10	rot	rot	VERB
biotropia-182	161	11	.	.	PUNCT
biotropia-182	162	1	final	final	ADJ
biotropia-182	162	2	report	report	NOUN
biotropia-182	162	3	,	,	PUNCT
biotropia-182	162	4	project	project	NOUN
biotropia-182	162	5	fv104b	fv104b	PROPN
biotropia-182	162	6	,	,	PUNCT
biotropia-182	162	7	horticultural	horticultural	ADJ
biotropia-182	162	8	development	development	NOUN
biotropia-182	162	9	council	council	PROPN
biotropia-182	162	10	,	,	PUNCT
biotropia-182	162	11	bradbourne	bradbourne	PROPN
biotropia-182	162	12	house	house	PROPN
biotropia-182	162	13	,	,	PUNCT
biotropia-182	162	14	east	east	PROPN
biotropia-182	162	15	mailing	mailing	NOUN
biotropia-182	162	16	,	,	PUNCT
biotropia-182	162	17	kent	kent	PROPN
biotropia-182	162	18	me	i	PRON
biotropia-182	162	19	19	19	NUM
biotropia-182	162	20	6dz	6dz	ADJ
biotropia-182	162	21	,	,	PUNCT
biotropia-182	162	22	uk	uk	PROPN
biotropia-182	162	23	.	.	PROPN
biotropia-182	162	24	cherain	cherain	PROPN
biotropia-182	162	25	,	,	PUNCT
biotropia-182	162	26	l.	l.	PROPN
biotropia-182	162	27	,	,	PUNCT
biotropia-182	162	28	z.	z.	PROPN
biotropia-182	162	29	ismailov	ismailov	PROPN
biotropia-182	162	30	,	,	PUNCT
biotropia-182	162	31	s.haran	s.haran	NOUN
biotropia-182	162	32	,	,	PUNCT
biotropia-182	162	33	and	and	CCONJ
biotropia-182	162	34	i.	i.	PROPN
biotropia-182	162	35	chet	chet	PROPN
biotropia-182	162	36	.	.	PROPN
biotropia-182	163	1	1995	1995	NUM
biotropia-182	163	2	.	.	PUNCT
biotropia-182	164	1	chitinolytic	chitinolytic	ADJ
biotropia-182	164	2	enterobacter	enterobacter	ADJ
biotropia-182	164	3	agglomerans	agglomeran	NOUN
biotropia-182	164	4	antagonistic	antagonistic	ADJ
biotropia-182	164	5	to	to	ADP
biotropia-182	164	6	fungal	fungal	ADJ
biotropia-182	164	7	plant	plant	NOUN
biotropia-182	164	8	pathogens	pathogen	NOUN
biotropia-182	164	9	.	.	PUNCT
biotropia-182	165	1	appl	appl	PROPN
biotropia-182	165	2	.	.	PUNCT
biotropia-182	166	1	environ	environ	PROPN
biotropia-182	166	2	.	.	PUNCT
biotropia-182	166	3	microbiol	microbiol	PROPN
biotropia-182	166	4	.	.	PUNCT
biotropia-182	167	1	61,1720	61,1720	PROPN
biotropia-182	167	2	-	-	PUNCT
biotropia-182	167	3	1726	1726	NUM
biotropia-182	167	4	.	.	PUNCT
biotropia-182	168	1	chernin	chernin	NOUN
biotropia-182	168	2	,	,	PUNCT
biotropia-182	168	3	l.s	l.s	PROPN
biotropia-182	168	4	.	.	PROPN
biotropia-182	168	5	,	,	PUNCT
biotropia-182	168	6	de	de	X
biotropia-182	168	7	la	la	X
biotropia-182	168	8	fuente	fuente	PROPN
biotropia-182	168	9	,	,	PUNCT
biotropia-182	168	10	l.	l.	PROPN
biotropia-182	168	11	,	,	PUNCT
biotropia-182	168	12	sobolev	sobolev	PROPN
biotropia-182	168	13	,	,	PUNCT
biotropia-182	168	14	v.	v.	PROPN
biotropia-182	168	15	,	,	PUNCT
biotropia-182	168	16	haran	haran	PROPN
biotropia-182	168	17	,	,	PUNCT
biotropia-182	168	18	s.	s.	PROPN
biotropia-182	168	19	,	,	PUNCT
biotropia-182	168	20	vorgias	vorgias	PROPN
biotropia-182	168	21	,	,	PUNCT
biotropia-182	168	22	c.e	c.e	PROPN
biotropia-182	168	23	.	.	PROPN
biotropia-182	168	24	,	,	PUNCT
biotropia-182	168	25	oppenheim	oppenheim	PROPN
biotropia-182	168	26	,	,	PUNCT
biotropia-182	168	27	a.b	a.b	PROPN
biotropia-182	168	28	.	.	PROPN
biotropia-182	168	29	and	and	CCONJ
biotropia-182	168	30	i.	i.	PROPN
biotropia-182	168	31	chet	chet	PROPN
biotropia-182	168	32	.	.	PROPN
biotropia-182	169	1	1997	1997	NUM
biotropia-182	169	2	.	.	PUNCT
biotropia-182	170	1	molecular	molecular	ADJ
biotropia-182	170	2	cloning	cloning	NOUN
biotropia-182	170	3	,	,	PUNCT
biotropia-182	170	4	structural	structural	ADJ
biotropia-182	170	5	analysis	analysis	NOUN
biotropia-182	170	6	,	,	PUNCT
biotropia-182	170	7	and	and	CCONJ
biotropia-182	170	8	expression	expression	NOUN
biotropia-182	170	9	in	in	ADP
biotropia-182	170	10	escherichia	escherichia	NOUN
biotropia-182	170	11	coli	coli	NOUN
biotropia-182	170	12	of	of	ADP
biotropia-182	170	13	a	a	DET
biotropia-182	170	14	chitinase	chitinase	NOUN
biotropia-182	170	15	gene	gene	NOUN
biotropia-182	170	16	from	from	ADP
biotropia-182	170	17	enterobacter	enterobacter	ADJ
biotropia-182	170	18	agglomerans	agglomeran	NOUN
biotropia-182	170	19	.	.	PUNCT
biotropia-182	171	1	appl	appl	PROPN
biotropia-182	171	2	.	.	PUNCT
biotropia-182	172	1	environ	environ	PROPN
biotropia-182	172	2	.	.	PUNCT
biotropia-182	172	3	microbiol	microbiol	PROPN
biotropia-182	172	4	.	.	PUNCT
biotropia-182	173	1	63	63	NUM
biotropia-182	173	2	,	,	PUNCT
biotropia-182	173	3	834	834	NUM
biotropia-182	173	4	-839	-839	PROPN
biotropia-182	173	5	.	.	PROPN
biotropia-182	173	6	46	46	NUM
biotropia-182	173	7	biotropia	biotropia	NOUN
biotropia-182	173	8	no	no	NOUN
biotropia-182	173	9	.	.	PUNCT
biotropia-182	174	1	20,2003	20,2003	NUM
biotropia-182	174	2	dennis	dennis	PROPN
biotropia-182	174	3	,	,	PUNCT
biotropia-182	174	4	j.j	j.j	PROPN
biotropia-182	174	5	.	.	PROPN
biotropia-182	174	6	and	and	CCONJ
biotropia-182	174	7	g.j	g.j	PROPN
biotropia-182	174	8	.	.	PROPN
biotropia-182	174	9	zylstra	zylstra	PROPN
biotropia-182	174	10	.	.	PUNCT
biotropia-182	174	11	1998	1998	NUM
biotropia-182	174	12	.	.	PUNCT
biotropia-182	175	1	plasposon	plasposon	NOUN
biotropia-182	175	2	:	:	PUNCT
biotropia-182	175	3	modular	modular	ADJ
biotropia-182	175	4	self	self	NOUN
biotropia-182	175	5	-	-	PUNCT
biotropia-182	175	6	cloning	cloning	NOUN
biotropia-182	175	7	minitransposon	minitransposon	NOUN
biotropia-182	175	8	derivatives	derivative	NOUN
biotropia-182	175	9	for	for	ADP
biotropia-182	175	10	rapid	rapid	ADJ
biotropia-182	175	11	genetic	genetic	ADJ
biotropia-182	175	12	analysis	analysis	NOUN
biotropia-182	175	13	of	of	ADP
biotropia-182	175	14	gram	gram	NOUN
biotropia-182	175	15	-	-	PUNCT
biotropia-182	175	16	negative	negative	ADJ
biotropia-182	175	17	bacterial	bacterial	ADJ
biotropia-182	175	18	genomes	genome	NOUN
biotropia-182	175	19	.	.	PUNCT
biotropia-182	176	1	appl	appl	PROPN
biotropia-182	176	2	.	.	PUNCT
biotropia-182	177	1	environ	environ	PROPN
biotropia-182	177	2	.	.	PUNCT
biotropia-182	177	3	microbiol	microbiol	PROPN
biotropia-182	177	4	.	.	PUNCT
biotropia-182	178	1	64,2710	64,2710	NUM
biotropia-182	178	2	-	-	PUNCT
biotropia-182	178	3	2715	2715	NUM
biotropia-182	178	4	.	.	PUNCT
biotropia-182	179	1	ditta	ditta	PROPN
biotropia-182	179	2	,	,	PUNCT
biotropia-182	179	3	g.	g.	PROPN
biotropia-182	179	4	,	,	PUNCT
biotropia-182	179	5	stanfield	stanfield	PROPN
biotropia-182	179	6	,	,	PUNCT
biotropia-182	179	7	s.	s.	PROPN
biotropia-182	179	8	,	,	PUNCT
biotropia-182	179	9	corbin	corbin	PROPN
biotropia-182	179	10	,	,	PUNCT
biotropia-182	179	11	d.	d.	PROPN
biotropia-182	179	12	and	and	CCONJ
biotropia-182	179	13	d.r	d.r	PROPN
biotropia-182	179	14	.	.	PROPN
biotropia-182	179	15	helsinski	helsinski	PROPN
biotropia-182	179	16	.	.	PUNCT
biotropia-182	180	1	1980	1980	NUM
biotropia-182	180	2	.	.	PUNCT
biotropia-182	181	1	broad	broad	ADJ
biotropia-182	181	2	-	-	PUNCT
biotropia-182	181	3	host	host	NOUN
biotropia-182	181	4	range	range	NOUN
biotropia-182	181	5	dna	dna	NOUN
biotropia-182	181	6	cloning	cloning	NOUN
biotropia-182	181	7	system	system	NOUN
biotropia-182	181	8	for	for	ADP
biotropia-182	181	9	gramnegative	gramnegative	ADJ
biotropia-182	181	10	bacteria	bacteria	NOUN
biotropia-182	181	11	:	:	PUNCT
biotropia-182	181	12	construction	construction	NOUN
biotropia-182	181	13	of	of	ADP
biotropia-182	181	14	gene	gene	NOUN
biotropia-182	181	15	bank	bank	PROPN
biotropia-182	181	16	of	of	ADP
biotropia-182	181	17	rhizobium	rhizobium	NOUN
biotropia-182	181	18	melihti	melihti	NOUN
biotropia-182	181	19	.	.	PUNCT
biotropia-182	182	1	proc	proc	NOUN
biotropia-182	182	2	.	.	PUNCT
biotropia-182	183	1	natl	natl	PROPN
biotropia-182	183	2	.	.	PUNCT
biotropia-182	184	1	acad	acad	PROPN
biotropia-182	184	2	.	.	PUNCT
biotropia-182	185	1	sci	sci	PROPN
biotropia-182	185	2	.	.	PUNCT
biotropia-182	186	1	77,7347	77,7347	NUM
biotropia-182	186	2	-	-	SYM
biotropia-182	186	3	7451	7451	NUM
biotropia-182	186	4	.	.	PUNCT
biotropia-182	187	1	downing	down	VERB
biotropia-182	187	2	,	,	PUNCT
biotropia-182	187	3	k.j	k.j	PROPN
biotropia-182	187	4	.	.	PROPN
biotropia-182	187	5	and	and	CCONJ
biotropia-182	187	6	j.a	j.a	PROPN
biotropia-182	187	7	.	.	PROPN
biotropia-182	187	8	thomson	thomson	PROPN
biotropia-182	187	9	.	.	PROPN
biotropia-182	187	10	2000	2000	NUM
biotropia-182	187	11	.	.	PUNCT
biotropia-182	188	1	introduction	introduction	NOUN
biotropia-182	188	2	of	of	ADP
biotropia-182	188	3	the	the	DET
biotropia-182	188	4	serralia	serralia	PROPN
biotropia-182	188	5	marcescensa	marcescensa	PROPN
biotropia-182	188	6	chia	chia	PROPN
biotropia-182	188	7	gene	gene	NOUN
biotropia-182	188	8	into	into	ADP
biotropia-182	188	9	an	an	DET
biotropia-182	188	10	endophytic	endophytic	ADJ
biotropia-182	188	11	pseudomonas	pseudomona	NOUN
biotropia-182	188	12	jluorescens	jluorescen	NOUN
biotropia-182	188	13	for	for	ADP
biotropia-182	188	14	the	the	DET
biotropia-182	188	15	biocontrol	biocontrol	NOUN
biotropia-182	188	16	of	of	ADP
biotropia-182	188	17	phytopathogenic	phytopathogenic	ADJ
biotropia-182	188	18	fungi	fungi	PROPN
biotropia-182	188	19	.	.	PUNCT
biotropia-182	189	1	can	can	AUX
biotropia-182	189	2	j	j	PROPN
biotropia-182	189	3	microbiol	microbiol	PROPN
biotropia-182	189	4	46	46	NUM
biotropia-182	189	5	,	,	PUNCT
biotropia-182	189	6	363	363	NUM
biotropia-182	189	7	-	-	SYM
biotropia-182	189	8	369	369	NUM
biotropia-182	189	9	.	.	PUNCT
biotropia-182	190	1	eisenstadt	eisenstadt	PROPN
biotropia-182	190	2	,	,	PUNCT
biotropia-182	190	3	e.	e.	PROPN
biotropia-182	190	4	,	,	PUNCT
biotropia-182	190	5	b.	b.	PROPN
biotropia-182	190	6	c.	c.	PROPN
biotropia-182	190	7	carlton	carlton	PROPN
biotropia-182	190	8	and	and	CCONJ
biotropia-182	190	9	b.	b.	PROPN
biotropia-182	190	10	j.	j.	PROPN
biotropia-182	190	11	brown	brown	PROPN
biotropia-182	190	12	.	.	PUNCT
biotropia-182	191	1	1994	1994	NUM
biotropia-182	191	2	.	.	PUNCT
biotropia-182	192	1	gene	gene	NOUN
biotropia-182	192	2	mutation	mutation	NOUN
biotropia-182	192	3	.	.	PUNCT
biotropia-182	193	1	in	in	ADP
biotropia-182	193	2	:	:	PUNCT
biotropia-182	193	3	gerhardt	gerhardt	ADJ
biotropia-182	193	4	,	,	PUNCT
biotropia-182	193	5	p.	p.	PROPN
biotropia-182	193	6	,	,	PUNCT
biotropia-182	193	7	r.	r.	PROPN
biotropia-182	193	8	g.	g.	PROPN
biotropia-182	193	9	e.	e.	PROPN
biotropia-182	193	10	murray	murray	PROPN
biotropia-182	193	11	,	,	PUNCT
biotropia-182	193	12	w.	w.	PROPN
biotropia-182	193	13	a.	a.	NOUN
biotropia-182	193	14	wood	wood	PROPN
biotropia-182	193	15	,	,	PUNCT
biotropia-182	193	16	n.r	n.r	PROPN
biotropia-182	193	17	.	.	PROPN
biotropia-182	193	18	krieg	krieg	PROPN
biotropia-182	193	19	(	(	PUNCT
biotropia-182	193	20	eds	ed	NOUN
biotropia-182	193	21	)	)	PUNCT
biotropia-182	193	22	.	.	PUNCT
biotropia-182	194	1	methods	method	NOUN
biotropia-182	194	2	for	for	ADP
biotropia-182	194	3	general	general	ADJ
biotropia-182	194	4	and	and	CCONJ
biotropia-182	194	5	molecular	molecular	ADJ
biotropia-182	194	6	bacteriology	bacteriology	NOUN
biotropia-182	194	7	.	.	PUNCT
biotropia-182	195	1	american	american	ADJ
biotropia-182	195	2	society	society	PROPN
biotropia-182	195	3	for	for	ADP
biotropia-182	195	4	microbiology	microbiology	NOUN
biotropia-182	195	5	,	,	PUNCT
biotropia-182	195	6	washington	washington	PROPN
biotropia-182	195	7	,	,	PUNCT
biotropia-182	195	8	d.c	d.c	PROPN
biotropia-182	195	9	.	.	PROPN
biotropia-182	195	10	:	:	PUNCT
biotropia-182	195	11	297	297	NUM
biotropia-182	195	12	-	-	SYM
biotropia-182	195	13	315	315	NUM
biotropia-182	195	14	.	.	PUNCT
biotropia-182	195	15	evrall	evrall	NOUN
biotropia-182	195	16	,	,	PUNCT
biotropia-182	195	17	c.c	c.c	PROPN
biotropia-182	195	18	.	.	PROPN
biotropia-182	195	19	,	,	PUNCT
biotropia-182	195	20	atwell	atwell	PROPN
biotropia-182	195	21	,	,	PUNCT
biotropia-182	195	22	r.w	r.w	PROPN
biotropia-182	195	23	.	.	PROPN
biotropia-182	195	24	,	,	PUNCT
biotropia-182	195	25	and	and	CCONJ
biotropia-182	195	26	c.a	c.a	PROPN
biotropia-182	195	27	.	.	PROPN
biotropia-182	195	28	smith	smith	PROPN
biotropia-182	195	29	.	.	PUNCT
biotropia-182	195	30	1990	1990	NUM
biotropia-182	195	31	.	.	PUNCT
biotropia-182	196	1	a	a	DET
biotropia-182	196	2	semi	semi	ADJ
biotropia-182	196	3	-	-	ADJ
biotropia-182	196	4	micro	micro	ADJ
biotropia-182	196	5	quantitative	quantitative	ADJ
biotropia-182	196	6	assay	assay	NOUN
biotropia-182	196	7	for	for	ADP
biotropia-182	196	8	determination	determination	NOUN
biotropia-182	196	9	of	of	ADP
biotropia-182	196	10	chitinolytic	chitinolytic	ADJ
biotropia-182	196	11	activity	activity	NOUN
biotropia-182	196	12	in	in	ADP
biotropia-182	196	13	microorganisms	microorganism	NOUN
biotropia-182	196	14	.	.	PUNCT
biotropia-182	197	1	j.	j.	PROPN
biotropia-182	197	2	microbiol	microbiol	PROPN
biotropia-182	197	3	.	.	PUNCT
biotropia-182	198	1	methods	method	NOUN
biotropia-182	198	2	12,183	12,183	NUM
biotropia-182	198	3	-	-	SYM
biotropia-182	198	4	187	187	NUM
biotropia-182	198	5	.	.	PUNCT
biotropia-182	199	1	gooday	gooday	NOUN
biotropia-182	199	2	,	,	PUNCT
biotropia-182	199	3	g.w	g.w	PROPN
biotropia-182	199	4	.	.	PROPN
biotropia-182	199	5	1990	1990	NUM
biotropia-182	199	6	.	.	PUNCT
biotropia-182	200	1	physiology	physiology	NOUN
biotropia-182	200	2	of	of	ADP
biotropia-182	200	3	microbial	microbial	ADJ
biotropia-182	200	4	degradation	degradation	NOUN
biotropia-182	200	5	of	of	ADP
biotropia-182	200	6	chitin	chitin	NOUN
biotropia-182	200	7	and	and	CCONJ
biotropia-182	200	8	chitosan	chitosan	NOUN
biotropia-182	200	9	.	.	PUNCT
biotropia-182	201	1	biodegradation	biodegradation	NOUN
biotropia-182	201	2	1	1	NUM
biotropia-182	201	3	,	,	PUNCT
biotropia-182	201	4	177	177	NUM
biotropia-182	201	5	-	-	SYM
biotropia-182	201	6	190	190	NUM
biotropia-182	201	7	.	.	PUNCT
biotropia-182	202	1	hedrick	hedrick	PROPN
biotropia-182	202	2	,	,	PUNCT
biotropia-182	202	3	s.a	s.a	PROPN
biotropia-182	202	4	.	.	PROPN
biotropia-182	202	5	,	,	PUNCT
biotropia-182	202	6	bell	bell	PROPN
biotropia-182	202	7	,	,	PUNCT
biotropia-182	202	8	j.n	j.n	PROPN
biotropia-182	202	9	..	..	PROPN
biotropia-182	202	10	,	,	PUNCT
biotropia-182	202	11	boiler	boiler	NOUN
biotropia-182	202	12	,	,	PUNCT
biotropia-182	202	13	t.	t.	PROPN
biotropia-182	202	14	and	and	CCONJ
biotropia-182	202	15	c.j	c.j	PROPN
biotropia-182	202	16	.	.	PROPN
biotropia-182	202	17	lamb	lamb	PROPN
biotropia-182	202	18	.	.	PUNCT
biotropia-182	203	1	1988	1988	NUM
biotropia-182	203	2	.	.	PUNCT
biotropia-182	204	1	chitinase	chitinase	NOUN
biotropia-182	204	2	cdna	cdna	PROPN
biotropia-182	204	3	cloning	cloning	PROPN
biotropia-182	204	4	and	and	CCONJ
biotropia-182	204	5	mrna	mrna	PROPN
biotropia-182	204	6	induction	induction	NOUN
biotropia-182	204	7	by	by	ADP
biotropia-182	204	8	fungal	fungal	ADJ
biotropia-182	204	9	elicitor	elicitor	NOUN
biotropia-182	204	10	,	,	PUNCT
biotropia-182	204	11	wounding	wounding	NOUN
biotropia-182	204	12	,	,	PUNCT
biotropia-182	204	13	and	and	CCONJ
biotropia-182	204	14	infection	infection	NOUN
biotropia-182	204	15	.	.	PUNCT
biotropia-182	205	1	plant	plant	NOUN
biotropia-182	205	2	physiol	physiol	PROPN
biotropia-182	205	3	.	.	PUNCT
biotropia-182	206	1	86,182	86,182	NUM
biotropia-182	206	2	-	-	SYM
biotropia-182	206	3	186	186	NUM
biotropia-182	206	4	.	.	PUNCT
biotropia-182	207	1	hood	hood	PROPN
biotropia-182	207	2	,	,	PUNCT
biotropia-182	207	3	m.a	m.a	PROPN
biotropia-182	207	4	.	.	PROPN
biotropia-182	207	5	1990	1990	NUM
biotropia-182	207	6	.	.	PUNCT
biotropia-182	208	1	comparison	comparison	NOUN
biotropia-182	208	2	of	of	ADP
biotropia-182	208	3	four	four	NUM
biotropia-182	208	4	methods	method	NOUN
biotropia-182	208	5	for	for	ADP
biotropia-182	208	6	measuring	measure	VERB
biotropia-182	208	7	chitinase	chitinase	NOUN
biotropia-182	208	8	activity	activity	NOUN
biotropia-182	208	9	and	and	CCONJ
biotropia-182	208	10	the	the	DET
biotropia-182	208	11	application	application	NOUN
biotropia-182	208	12	of	of	ADP
biotropia-182	208	13	the	the	DET
biotropia-182	208	14	4	4	NUM
biotropia-182	208	15	-	-	PUNCT
biotropia-182	208	16	muf	muf	NOUN
biotropia-182	208	17	assay	assay	NOUN
biotropia-182	208	18	in	in	ADP
biotropia-182	208	19	aquatic	aquatic	ADJ
biotropia-182	208	20	environments	environment	NOUN
biotropia-182	208	21	.	.	PUNCT
biotropia-182	209	1	j.	j.	PROPN
biotropia-182	209	2	microbiol	microbiol	PROPN
biotropia-182	209	3	.	.	PUNCT
biotropia-182	210	1	methods	method	NOUN
biotropia-182	210	2	13	13	NUM
biotropia-182	210	3	,	,	PUNCT
biotropia-182	210	4	51	51	NUM
biotropia-182	210	5	-	-	SYM
biotropia-182	210	6	160	160	NUM
biotropia-182	210	7	.	.	PUNCT
biotropia-182	211	1	hsu	hsu	PROPN
biotropia-182	211	2	,	,	PUNCT
biotropia-182	211	3	s.c	s.c	PROPN
biotropia-182	211	4	.	.	PROPN
biotropia-182	211	5	and	and	CCONJ
biotropia-182	211	6	j.l	j.l	PROPN
biotropia-182	211	7	.	.	PROPN
biotropia-182	211	8	lockwood	lockwood	PROPN
biotropia-182	211	9	.	.	PUNCT
biotropia-182	212	1	.1975	.1975	PROPN
biotropia-182	212	2	.	.	PUNCT
biotropia-182	213	1	powdered	powdered	ADJ
biotropia-182	213	2	chitin	chitin	NOUN
biotropia-182	213	3	agar	agar	NOUN
biotropia-182	213	4	as	as	ADP
biotropia-182	213	5	a	a	DET
biotropia-182	213	6	selective	selective	ADJ
biotropia-182	213	7	medium	medium	NOUN
biotropia-182	213	8	for	for	ADP
biotropia-182	213	9	enumeration	enumeration	NOUN
biotropia-182	213	10	of	of	ADP
biotropia-182	213	11	actinomycetes	actinomycete	NOUN
biotropia-182	213	12	in	in	ADP
biotropia-182	213	13	water	water	NOUN
biotropia-182	213	14	and	and	CCONJ
biotropia-182	213	15	soil	soil	NOUN
biotropia-182	213	16	.	.	PUNCT
biotropia-182	214	1	appl	appl	PROPN
biotropia-182	214	2	.	.	PUNCT
biotropia-182	215	1	microbiol	microbiol	PROPN
biotropia-182	215	2	.	.	PUNCT
biotropia-182	216	1	29	29	NUM
biotropia-182	216	2	,	,	PUNCT
biotropia-182	216	3	422	422	NUM
biotropia-182	216	4	-	-	SYM
biotropia-182	216	5	426	426	NUM
biotropia-182	216	6	inbar	inbar	NOUN
biotropia-182	216	7	,	,	PUNCT
biotropia-182	216	8	j	j	PROPN
biotropia-182	216	9	and	and	CCONJ
biotropia-182	216	10	i.	i.	PROPN
biotropia-182	216	11	chet	chet	PROPN
biotropia-182	216	12	.	.	PROPN
biotropia-182	217	1	1991	1991	NUM
biotropia-182	217	2	.	.	PUNCT
biotropia-182	218	1	evidence	evidence	NOUN
biotropia-182	218	2	that	that	PRON
biotropia-182	218	3	chitinase	chitinase	NOUN
biotropia-182	218	4	produced	produce	VERB
biotropia-182	218	5	by	by	ADP
biotropia-182	218	6	aeromonas	aeromonas	PROPN
biotropia-182	218	7	caviae	caviae	PROPN
biotropia-182	218	8	is	be	AUX
biotropia-182	218	9	involved	involve	VERB
biotropia-182	218	10	in	in	ADP
biotropia-182	218	11	the	the	DET
biotropia-182	218	12	biological	biological	ADJ
biotropia-182	218	13	control	control	NOUN
biotropia-182	218	14	of	of	ADP
biotropia-182	218	15	soil	soil	NOUN
biotropia-182	218	16	-	-	PUNCT
biotropia-182	218	17	borne	bear	VERB
biotropia-182	218	18	plant	plant	NOUN
biotropia-182	218	19	pathogens	pathogen	NOUN
biotropia-182	218	20	by	by	ADP
biotropia-182	218	21	this	this	DET
biotropia-182	218	22	bacterium	bacterium	NOUN
biotropia-182	218	23	.	.	PUNCT
biotropia-182	219	1	soil	soil	NOUN
biotropia-182	219	2	biol	biol	PROPN
biotropia-182	219	3	.	.	PUNCT
biotropia-182	220	1	biochem	biochem	PROPN
biotropia-182	220	2	.	.	PUNCT
biotropia-182	221	1	23	23	NUM
biotropia-182	221	2	,	,	PUNCT
biotropia-182	221	3	973	973	NUM
biotropia-182	221	4	-	-	SYM
biotropia-182	221	5	978	978	NUM
biotropia-182	221	6	.	.	PUNCT
biotropia-182	222	1	jones	jones	PROPN
biotropia-182	222	2	,	,	PUNCT
biotropia-182	222	3	j.d.g	j.d.g	PROPN
biotropia-182	222	4	.	.	PUNCT
biotropia-182	222	5	,	,	PUNCT
biotropia-182	222	6	k.l.grady	k.l.grady	PROPN
biotropia-182	222	7	,	,	PUNCT
biotropia-182	222	8	t.v.suslow	t.v.suslow	VERB
biotropia-182	222	9	,	,	PUNCT
biotropia-182	222	10	and	and	CCONJ
biotropia-182	222	11	j.r.bedbrook	j.r.bedbrook	NOUN
biotropia-182	222	12	.	.	PUNCT
biotropia-182	223	1	1986	1986	NUM
biotropia-182	223	2	.	.	PUNCT
biotropia-182	224	1	isolation	isolation	NOUN
biotropia-182	224	2	and	and	CCONJ
biotropia-182	224	3	characterization	characterization	NOUN
biotropia-182	224	4	of	of	ADP
biotropia-182	224	5	genes	gene	NOUN
biotropia-182	224	6	encoding	encode	VERB
biotropia-182	224	7	two	two	NUM
biotropia-182	224	8	chitinase	chitinase	NOUN
biotropia-182	224	9	enzymes	enzyme	NOUN
biotropia-182	224	10	from	from	ADP
biotropia-182	224	11	serratia	serratia	PROPN
biotropia-182	224	12	marcescens	marcescens	PROPN
biotropia-182	224	13	.	.	PUNCT
biotropia-182	225	1	embo	embo	PROPN
biotropia-182	225	2	j.	j.	PROPN
biotropia-182	225	3	5	5	NUM
biotropia-182	225	4	:	:	PUNCT
biotropia-182	225	5	467	467	NUM
biotropia-182	225	6	-	-	SYM
biotropia-182	225	7	473	473	NUM
biotropia-182	225	8	.	.	PUNCT
biotropia-182	226	1	king	king	PROPN
biotropia-182	226	2	,	,	PUNCT
biotropia-182	226	3	e.o	e.o	PROPN
biotropia-182	226	4	.	.	PROPN
biotropia-182	226	5	,	,	PUNCT
biotropia-182	226	6	ward	ward	PROPN
biotropia-182	226	7	,	,	PUNCT
biotropia-182	226	8	m.k	m.k	PROPN
biotropia-182	226	9	.	.	PROPN
biotropia-182	226	10	and	and	CCONJ
biotropia-182	226	11	d.e	d.e	PROPN
biotropia-182	226	12	.	.	PROPN
biotropia-182	226	13	raney	raney	PROPN
biotropia-182	226	14	.	.	PUNCT
biotropia-182	226	15	1954	1954	NUM
biotropia-182	226	16	.	.	PUNCT
biotropia-182	227	1	two	two	NUM
biotropia-182	227	2	simple	simple	ADJ
biotropia-182	227	3	media	medium	NOUN
biotropia-182	227	4	for	for	ADP
biotropia-182	227	5	the	the	DET
biotropia-182	227	6	demonstration	demonstration	NOUN
biotropia-182	227	7	of	of	ADP
biotropia-182	227	8	pyocyanin	pyocyanin	NOUN
biotropia-182	227	9	and	and	CCONJ
biotropia-182	227	10	fluoreescin	fluoreescin	PRON
biotropia-182	227	11	.	.	PUNCT
biotropia-182	228	1	j.	j.	PROPN
biotropia-182	228	2	labor	labor	PROPN
biotropia-182	228	3	.	.	PUNCT
biotropia-182	229	1	clin	clin	PROPN
biotropia-182	229	2	.	.	PUNCT
biotropia-182	230	1	med	med	PROPN
biotropia-182	230	2	.	.	PROPN
biotropia-182	230	3	44	44	NUM
biotropia-182	230	4	,	,	PUNCT
biotropia-182	230	5	301	301	NUM
biotropia-182	230	6	-	-	SYM
biotropia-182	230	7	307	307	NUM
biotropia-182	230	8	.	.	PUNCT
biotropia-182	231	1	koby	koby	PROPN
biotropia-182	231	2	,	,	PUNCT
biotropia-182	231	3	s.	s.	PROPN
biotropia-182	231	4	,	,	PUNCT
biotropia-182	231	5	schickler	schickler	PROPN
biotropia-182	231	6	,	,	PUNCT
biotropia-182	231	7	h.	h.	PROPN
biotropia-182	231	8	,	,	PUNCT
biotropia-182	231	9	chet	chet	PROPN
biotropia-182	231	10	,	,	PUNCT
biotropia-182	231	11	i.	i.	PROPN
biotropia-182	231	12	and	and	CCONJ
biotropia-182	231	13	a.b	a.b	PROPN
biotropia-182	231	14	.	.	PROPN
biotropia-182	231	15	oppenheim	oppenheim	PROPN
biotropia-182	231	16	.	.	PUNCT
biotropia-182	231	17	1994	1994	NUM
biotropia-182	231	18	.	.	PUNCT
biotropia-182	232	1	the	the	DET
biotropia-182	232	2	chitinase	chitinase	NOUN
biotropia-182	232	3	encoding	encode	VERB
biotropia-182	232	4	tn7	tn7	NOUN
biotropia-182	232	5	-	-	PUNCT
biotropia-182	232	6	based	base	VERB
biotropia-182	232	7	chia	chia	NOUN
biotropia-182	232	8	gene	gene	NOUN
biotropia-182	232	9	endows	endow	VERB
biotropia-182	232	10	pseudomonas	pseudomona	NOUN
biotropia-182	232	11	jluorescens	jluorescen	NOUN
biotropia-182	232	12	with	with	ADP
biotropia-182	232	13	the	the	DET
biotropia-182	232	14	capacity	capacity	NOUN
biotropia-182	232	15	to	to	PART
biotropia-182	232	16	control	control	VERB
biotropia-182	232	17	plant	plant	NOUN
biotropia-182	232	18	pathogens	pathogen	NOUN
biotropia-182	232	19	in	in	ADP
biotropia-182	232	20	soil	soil	NOUN
biotropia-182	232	21	.	.	PUNCT
biotropia-182	233	1	gene	gene	NOUN
biotropia-182	233	2	147,81	147,81	NOUN
biotropia-182	233	3	-	-	SYM
biotropia-182	233	4	83	83	NUM
biotropia-182	233	5	.	.	PUNCT
biotropia-182	234	1	kovach	kovach	PROPN
biotropia-182	234	2	,	,	PUNCT
biotropia-182	234	3	m.e	m.e	PROPN
biotropia-182	234	4	.	.	PROPN
biotropia-182	234	5	,	,	PUNCT
biotropia-182	234	6	phillips	phillips	PROPN
biotropia-182	234	7	,	,	PUNCT
biotropia-182	234	8	r.w	r.w	PROPN
biotropia-182	234	9	.	.	PROPN
biotropia-182	234	10	,	,	PUNCT
biotropia-182	234	11	elzer	elzer	PROPN
biotropia-182	234	12	,	,	PUNCT
biotropia-182	234	13	p.h	p.h	PROPN
biotropia-182	234	14	.	.	PROPN
biotropia-182	234	15	,	,	PUNCT
biotropia-182	234	16	roop	roop	PROPN
biotropia-182	234	17	ii	ii	PROPN
biotropia-182	234	18	,	,	PUNCT
biotropia-182	234	19	r.m	r.m	PROPN
biotropia-182	234	20	.	.	PROPN
biotropia-182	234	21	and	and	CCONJ
biotropia-182	234	22	k.m	k.m	PROPN
biotropia-182	234	23	.	.	PROPN
biotropia-182	234	24	peterson	peterson	PROPN
biotropia-182	234	25	.	.	PROPN
biotropia-182	234	26	1994	1994	NUM
biotropia-182	234	27	.	.	PUNCT
biotropia-182	235	1	pbbrlmcs	pbbrlmcs	NOUN
biotropia-182	235	2	:	:	PUNCT
biotropia-182	235	3	a	a	DET
biotropia-182	235	4	broad	broad	ADJ
biotropia-182	235	5	-	-	PUNCT
biotropia-182	235	6	host	host	NOUN
biotropia-182	235	7	range	range	NOUN
biotropia-182	235	8	cloning	cloning	NOUN
biotropia-182	235	9	vector	vector	NOUN
biotropia-182	235	10	.	.	PUNCT
biotropia-182	236	1	biotechniques	biotechnique	NOUN
biotropia-182	236	2	16	16	NUM
biotropia-182	236	3	,	,	PUNCT
biotropia-182	236	4	800	800	NUM
biotropia-182	236	5	-	-	SYM
biotropia-182	236	6	802	802	NUM
biotropia-182	236	7	.	.	PUNCT
biotropia-182	236	8	malik	malik	PROPN
biotropia-182	236	9	,	,	PUNCT
biotropia-182	236	10	a.	a.	PROPN
biotropia-182	236	11	,	,	PUNCT
biotropia-182	236	12	wenuganen	wenuganen	NOUN
biotropia-182	236	13	,	,	PUNCT
biotropia-182	236	14	s.	s.	PROPN
biotropia-182	236	15	,	,	PUNCT
biotropia-182	236	16	suwanto	suwanto	PROPN
biotropia-182	236	17	,	,	PUNCT
biotropia-182	236	18	a.	a.	NOUN
biotropia-182	236	19	and	and	CCONJ
biotropia-182	236	20	b.	b.	PROPN
biotropia-182	236	21	tjahjono	tjahjono	PROPN
biotropia-182	236	22	.	.	PUNCT
biotropia-182	237	1	cloning	cloning	NOUN
biotropia-182	237	2	,	,	PUNCT
biotropia-182	237	3	dna	dna	NOUN
biotropia-182	237	4	sequence	sequence	NOUN
biotropia-182	237	5	and	and	CCONJ
biotropia-182	237	6	expression	expression	NOUN
biotropia-182	237	7	of	of	ADP
biotropia-182	237	8	aeromonas	aeromonas	PROPN
biotropia-182	237	9	caviae	caviae	PROPN
biotropia-182	237	10	chitinase	chitinase	PROPN
biotropia-182	237	11	gene	gene	NOUN
biotropia-182	237	12	.	.	PUNCT
biotropia-182	238	1	molec	molec	PROPN
biotropia-182	238	2	.	.	PUNCT
biotropia-182	238	3	biotech	biotech	PROPN
biotropia-182	238	4	.	.	PUNCT
biotropia-182	239	1	(	(	PUNCT
biotropia-182	239	2	in	in	ADP
biotropia-182	239	3	press	press	NOUN
biotropia-182	239	4	)	)	PUNCT
biotropia-182	239	5	oka	oka	PROPN
biotropia-182	239	6	,	,	PUNCT
biotropia-182	239	7	a.	a.	NOUN
biotropia-182	239	8	,	,	PUNCT
biotropia-182	239	9	sugisaki	sugisaki	NOUN
biotropia-182	239	10	,	,	PUNCT
biotropia-182	239	11	h.	h.	PROPN
biotropia-182	239	12	and	and	CCONJ
biotropia-182	239	13	m.	m.	NOUN
biotropia-182	239	14	takanami	takanami	NOUN
biotropia-182	239	15	.	.	PUNCT
biotropia-182	240	1	1981	1981	NUM
biotropia-182	240	2	.	.	PUNCT
biotropia-182	241	1	nucleotide	nucleotide	NOUN
biotropia-182	241	2	sequence	sequence	NOUN
biotropia-182	241	3	of	of	ADP
biotropia-182	241	4	the	the	DET
biotropia-182	241	5	kanamycin	kanamycin	PROPN
biotropia-182	241	6	resistance	resistance	NOUN
biotropia-182	241	7	transposon	transposon	NOUN
biotropia-182	241	8	tn905	tn905	PROPN
biotropia-182	241	9	.	.	PUNCT
biotropia-182	242	1	mol	mol	PROPN
biotropia-182	242	2	.	.	PUNCT
biotropia-182	243	1	biol	biol	PROPN
biotropia-182	243	2	147	147	NUM
biotropia-182	243	3	:	:	PUNCT
biotropia-182	243	4	217	217	NUM
biotropia-182	243	5	-	-	SYM
biotropia-182	243	6	226	226	NUM
biotropia-182	243	7	.	.	PUNCT
biotropia-182	244	1	oppenheim	oppenheim	PROPN
biotropia-182	244	2	,	,	PUNCT
biotropia-182	244	3	a.	a.	PROPN
biotropia-182	244	4	b.	b.	PROPN
biotropia-182	244	5	and	and	CCONJ
biotropia-182	244	6	i.	i.	PROPN
biotropia-182	244	7	chet	chet	PROPN
biotropia-182	244	8	.	.	PROPN
biotropia-182	244	9	1992	1992	NUM
biotropia-182	244	10	.	.	PUNCT
biotropia-182	245	1	cloned	clone	VERB
biotropia-182	245	2	chitinases	chitinase	NOUN
biotropia-182	245	3	in	in	ADP
biotropia-182	245	4	fungal	fungal	ADJ
biotropia-182	245	5	plant	plant	NOUN
biotropia-182	245	6	pathogen	pathogen	NOUN
biotropia-182	245	7	control	control	NOUN
biotropia-182	245	8	strategies	strategy	NOUN
biotropia-182	245	9	.	.	PUNCT
biotropia-182	246	1	trends	trend	NOUN
biotropia-182	246	2	biotechnol	biotechnol	VERB
biotropia-182	246	3	.	.	PUNCT
biotropia-182	247	1	10,392	10,392	NUM
biotropia-182	247	2	-	-	SYM
biotropia-182	247	3	394	394	NUM
biotropia-182	247	4	.	.	PUNCT
biotropia-182	247	5	sambrook	sambrook	PROPN
biotropia-182	247	6	,	,	PUNCT
biotropia-182	247	7	j.	j.	PROPN
biotropia-182	247	8	,	,	PUNCT
biotropia-182	247	9	fritsch	fritsch	PROPN
biotropia-182	247	10	,	,	PUNCT
biotropia-182	247	11	e.f	e.f	PROPN
biotropia-182	247	12	.	.	PROPN
biotropia-182	247	13	and	and	CCONJ
biotropia-182	247	14	t.	t.	PROPN
biotropia-182	247	15	maniatis	maniatis	PROPN
biotropia-182	247	16	.	.	PUNCT
biotropia-182	247	17	1989	1989	NUM
biotropia-182	247	18	.	.	PUNCT
biotropia-182	248	1	molecular	molecular	ADJ
biotropia-182	248	2	cloning	cloning	NOUN
biotropia-182	248	3	.	.	PUNCT
biotropia-182	249	1	cold	cold	ADJ
biotropia-182	249	2	spring	spring	NOUN
biotropia-182	249	3	harbor	harbor	PROPN
biotropia-182	249	4	laboratory	laboratory	PROPN
biotropia-182	249	5	press	press	PROPN
biotropia-182	249	6	.	.	PUNCT
biotropia-182	250	1	cold	cold	ADJ
biotropia-182	250	2	spring	spring	NOUN
biotropia-182	250	3	harbor	harbor	NOUN
biotropia-182	250	4	.	.	PUNCT
biotropia-182	251	1	new	new	PROPN
biotropia-182	251	2	york	york	PROPN
biotropia-182	251	3	.	.	PUNCT
biotropia-182	252	1	usa	usa	PROPN
biotropia-182	252	2	47	47	NUM
biotropia-182	252	3	heterologous	heterologous	ADJ
biotropia-182	252	4	expression	expression	NOUN
biotropia-182	252	5	of	of	ADP
biotropia-182	252	6	a	a	DET
biotropia-182	252	7	chitinase	chitinase	NOUN
biotropia-182	252	8	gene	gene	NOUN
biotropia-182	252	9	from	from	ADP
biotropia-182	252	10	aeromonas	aeromonas	PROPN
biotropia-182	252	11	caviae	caviae	PROPN
biotropia-182	252	12	amarila	amarila	PROPN
biotropia-182	252	13	malik	malik	PROPN
biotropia-182	252	14	el	el	PROPN
biotropia-182	252	15	al	al	PROPN
biotropia-182	252	16	.	.	PROPN
biotropia-182	252	17	sitrit	sitrit	PROPN
biotropia-182	252	18	,	,	PUNCT
biotropia-182	252	19	y.	y.	PROPN
biotropia-182	252	20	,	,	PUNCT
biotropia-182	252	21	vorgias	vorgias	PROPN
biotropia-182	252	22	,	,	PUNCT
biotropia-182	252	23	c.e	c.e	PROPN
biotropia-182	252	24	.	.	PROPN
biotropia-182	252	25	,	,	PUNCT
biotropia-182	252	26	chet	chet	PROPN
biotropia-182	252	27	,	,	PUNCT
biotropia-182	252	28	i.	i.	PROPN
biotropia-182	252	29	and	and	CCONJ
biotropia-182	252	30	a.b	a.b	PROPN
biotropia-182	252	31	.	.	PROPN
biotropia-182	252	32	oppenherm	oppenherm	PROPN
biotropia-182	252	33	.	.	PUNCT
biotropia-182	253	1	1995	1995	NUM
biotropia-182	253	2	.	.	PUNCT
biotropia-182	254	1	cloning	cloning	NOUN
biotropia-182	254	2	and	and	CCONJ
biotropia-182	254	3	primary	primary	ADJ
biotropia-182	254	4	structure	structure	NOUN
biotropia-182	254	5	of	of	ADP
biotropia-182	254	6	the	the	DET
biotropia-182	254	7	chia	chia	NOUN
biotropia-182	254	8	gene	gene	NOUN
biotropia-182	254	9	from	from	ADP
biotropia-182	254	10	aeromonas	aeromonas	PROPN
biotropia-182	254	11	caviae	caviae	PROPN
biotropia-182	254	12	.	.	PUNCT
biotropia-182	255	1	j.	j.	PROPN
biotropia-182	255	2	bacteriol	bacteriol	PROPN
biotropia-182	255	3	.	.	PUNCT
biotropia-182	256	1	177	177	NUM
biotropia-182	256	2	,	,	PUNCT
biotropia-182	256	3	4187	4187	NUM
biotropia-182	256	4	-	-	SYM
biotropia-182	256	5	4189	4189	NUM
biotropia-182	256	6	sundheim	sundheim	NOUN
biotropia-182	256	7	,	,	PUNCT
biotropia-182	256	8	l.	l.	PROPN
biotropia-182	256	9	,	,	PUNCT
biotropia-182	256	10	a.r	a.r	PROPN
biotropia-182	256	11	.	.	PROPN
biotropia-182	256	12	poplawsky	poplawsky	PROPN
biotropia-182	256	13	and	and	CCONJ
biotropia-182	256	14	a.h	a.h	PROPN
biotropia-182	256	15	.	.	PROPN
biotropia-182	256	16	ellingboe	ellingboe	PROPN
biotropia-182	256	17	.	.	PUNCT
biotropia-182	257	1	1988	1988	NUM
biotropia-182	257	2	.	.	PUNCT
biotropia-182	258	1	molecular	molecular	ADJ
biotropia-182	258	2	cloning	cloning	NOUN
biotropia-182	258	3	of	of	ADP
biotropia-182	258	4	two	two	NUM
biotropia-182	258	5	chitinase	chitinase	NOUN
biotropia-182	258	6	genes	gene	NOUN
biotropia-182	258	7	from	from	ADP
biotropia-182	258	8	serratia	serratia	PROPN
biotropia-182	258	9	marcescens	marcescen	NOUN
biotropia-182	258	10	and	and	CCONJ
biotropia-182	258	11	their	their	PRON
biotropia-182	258	12	expression	expression	NOUN
biotropia-182	258	13	in	in	ADP
biotropia-182	258	14	pseudomonas	pseudomona	NOUN
biotropia-182	258	15	species	specie	NOUN
biotropia-182	258	16	.	.	PUNCT
biotropia-182	259	1	physiol	physiol	PROPN
biotropia-182	259	2	.	.	PUNCT
biotropia-182	260	1	mol	mol	PROPN
biotropia-182	260	2	.	.	PROPN
biotropia-182	260	3	plant	plant	NOUN
biotropia-182	260	4	.	.	PUNCT
biotropia-182	261	1	pathol	pathol	NOUN
biotropia-182	261	2	.	.	PUNCT
biotropia-182	262	1	33	33	NUM
biotropia-182	262	2	:	:	SYM
biotropia-182	262	3	483	483	NUM
biotropia-182	262	4	-	-	SYM
biotropia-182	262	5	491	491	NUM
biotropia-182	262	6	.	.	PUNCT
biotropia-182	263	1	suwanto	suwanto	PROPN
biotropia-182	263	2	,	,	PUNCT
biotropia-182	263	3	a.	a.	NOUN
biotropia-182	263	4	and	and	CCONJ
biotropia-182	263	5	s.	s.	PROPN
biotropia-182	263	6	kaplan	kaplan	PROPN
biotropia-182	263	7	.	.	PROPN
biotropia-182	263	8	1992	1992	NUM
biotropia-182	263	9	.	.	PUNCT
biotropia-182	264	1	a	a	DET
biotropia-182	264	2	self	self	NOUN
biotropia-182	264	3	-	-	PUNCT
biotropia-182	264	4	transmissable	transmissable	ADJ
biotropia-182	264	5	narrow	narrow	ADJ
biotropia-182	264	6	-	-	PUNCT
biotropia-182	264	7	host	host	NOUN
biotropia-182	264	8	range	range	NOUN
biotropia-182	264	9	endogenous	endogenous	ADJ
biotropia-182	264	10	plasmid	plasmid	NOUN
biotropia-182	264	11	of	of	ADP
biotropia-182	264	12	rhodobacter	rhodobacter	NOUN
biotropia-182	264	13	sphaeroides	sphaeroide	NOUN
biotropia-182	264	14	2.4.1	2.4.1	NUM
biotropia-182	264	15	:	:	PUNCT
biotropia-182	264	16	physical	physical	ADJ
biotropia-182	264	17	structure	structure	NOUN
biotropia-182	264	18	,	,	PUNCT
biotropia-182	264	19	incompatibility	incompatibility	NOUN
biotropia-182	264	20	determinants	determinant	NOUN
biotropia-182	264	21	,	,	PUNCT
biotropia-182	264	22	origin	origin	NOUN
biotropia-182	264	23	of	of	ADP
biotropia-182	264	24	replication	replication	NOUN
biotropia-182	264	25	,	,	PUNCT
biotropia-182	264	26	and	and	CCONJ
biotropia-182	264	27	transfer	transfer	NOUN
biotropia-182	264	28	function	function	NOUN
biotropia-182	264	29	.	.	PUNCT
biotropia-182	265	1	j.	j.	PROPN
biotropia-182	265	2	bacteriol	bacteriol	PROPN
biotropia-182	265	3	.	.	PUNCT
biotropia-182	266	1	174	174	NUM
biotropia-182	266	2	,	,	PUNCT
biotropia-182	266	3	1124	1124	NUM
biotropia-182	266	4	-	-	SYM
biotropia-182	266	5	113	113	NUM
biotropia-182	266	6	wirth	wirth	PROPN
biotropia-182	266	7	,	,	PUNCT
biotropia-182	266	8	s.j	s.j	PROPN
biotropia-182	266	9	.	.	PROPN
biotropia-182	266	10	and	and	CCONJ
biotropia-182	266	11	wolf	wolf	PROPN
biotropia-182	266	12	,	,	PUNCT
biotropia-182	266	13	g.a	g.a	PROPN
biotropia-182	266	14	.	.	PROPN
biotropia-182	266	15	(	(	PUNCT
biotropia-182	266	16	1990	1990	NUM
biotropia-182	266	17	.	.	PUNCT
biotropia-182	267	1	dye	dye	NOUN
biotropia-182	267	2	-	-	PUNCT
biotropia-182	267	3	labelled	label	VERB
biotropia-182	267	4	substrates	substrate	NOUN
biotropia-182	267	5	for	for	ADP
biotropia-182	267	6	the	the	DET
biotropia-182	267	7	assay	assay	NOUN
biotropia-182	267	8	and	and	CCONJ
biotropia-182	267	9	detection	detection	NOUN
biotropia-182	267	10	of	of	ADP
biotropia-182	267	11	chitinase	chitinase	NOUN
biotropia-182	267	12	and	and	CCONJ
biotropia-182	267	13	lysozyme	lysozyme	NOUN
biotropia-182	267	14	activity	activity	NOUN
biotropia-182	267	15	.	.	PUNCT
biotropia-182	268	1	j	j	PROPN
biotropia-182	268	2	microbiol	microbiol	PROPN
biotropia-182	268	3	.	.	PUNCT
biotropia-182	269	1	methods	method	NOUN
biotropia-182	269	2	12,197	12,197	NUM
biotropia-182	269	3	-	-	SYM
biotropia-182	269	4	205	205	NUM
biotropia-182	269	5	.	.	PUNCT
biotropia-182	270	1	48	48	NUM
biotropia-182	270	2	36.pdf	36.pdf	PROPN
biotropia-182	270	3	37.pdf	37.pdf	PROPN
biotropia-182	270	4	38.pdf	38.pdf	PROPN
biotropia-182	270	5	39.pdf	39.pdf	PROPN
biotropia-182	270	6	40.pdf	40.pdf	PROPN
biotropia-182	270	7	41.pdf	41.pdf	PROPN
biotropia-182	270	8	42.pdf	42.pdf	PROPN
biotropia-182	270	9	43.pdf	43.pdf	PROPN
biotropia-182	270	10	44.pdf	44.pdf	PROPN
biotropia-182	270	11	45.pdf	45.pdf	PROPN
biotropia-182	270	12	46.pdf	46.pdf	PROPN
biotropia-182	270	13	47.pdf	47.pdf	PROPN
biotropia-182	270	14	48.pdf	48.pdf	PROPN