id	sid	tid	token	lemma	pos
biotropia-204	1	1	microsoft	microsoft	PROPN
biotropia-204	1	2	word	word	PROPN
biotropia-204	1	3	29	29	NUM
biotropia-204	1	4	bio	bio	PROPN
biotropia-204	1	5	fropia	fropia	PROPN
biotropia-204	1	6	no	no	PROPN
biotropia-204	1	7	.	.	PROPN
biotropia-204	1	8	22	22	NUM
biotropia-204	1	9	,	,	PUNCT
biotropia-204	1	10	2004	2004	NUM
biotropia-204	1	11	:	:	PUNCT
biotropia-204	1	12	29	29	NUM
biotropia-204	1	13	-	-	SYM
biotropia-204	1	14	39	39	NUM
biotropia-204	1	15	isolation	isolation	NOUN
biotropia-204	1	16	and	and	CCONJ
biotropia-204	1	17	selection	selection	NOUN
biotropia-204	1	18	of	of	ADP
biotropia-204	1	19	alkaline	alkaline	ADJ
biotropia-204	1	20	proteolytic	proteolytic	ADJ
biotropia-204	1	21	bacteria	bacteria	NOUN
biotropia-204	1	22	from	from	ADP
biotropia-204	1	23	leather	leather	NOUN
biotropia-204	1	24	processing	processing	NOUN
biotropia-204	1	25	waste	waste	NOUN
biotropia-204	1	26	and	and	CCONJ
biotropia-204	1	27	enzyme	enzyme	NOUN
biotropia-204	1	28	characterization	characterization	NOUN
biotropia-204	1	29	budiasih	budiasih	NOUN
biotropia-204	1	30	wahyuntari	wahyuntari	PROPN
biotropia-204	1	31	'	'	PART
biotropia-204	1	32	,	,	PUNCT
biotropia-204	1	33	nisa	nisa	PROPN
biotropia-204	1	34	r	r	PROPN
biotropia-204	1	35	mubarikand	mubarikand	PROPN
biotropia-204	1	36	marita	marita	PROPN
biotropia-204	1	37	anggarani2	anggarani2	PROPN
biotropia-204	1	38	agency	agency	NOUN
biotropia-204	1	39	for	for	ADP
biotropia-204	1	40	the	the	DET
biotropia-204	1	41	assessment	assessment	NOUN
biotropia-204	1	42	&	&	CCONJ
biotropia-204	1	43	application	application	NOUN
biotropia-204	1	44	of	of	ADP
biotropia-204	1	45	technology	technology	NOUN
biotropia-204	1	46	,	,	PUNCT
biotropia-204	1	47	jakarta	jakarta	PROPN
biotropia-204	1	48	,	,	PUNCT
biotropia-204	1	49	indonesia	indonesia	PROPN
biotropia-204	1	50	²bogor	²bogor	PROPN
biotropia-204	1	51	agricultural	agricultural	ADJ
biotropia-204	1	52	university	university	NOUN
biotropia-204	1	53	,	,	PUNCT
biotropia-204	1	54	bogor	bogor	PROPN
biotropia-204	1	55	,	,	PUNCT
biotropia-204	1	56	indonesia	indonesia	PROPN
biotropia-204	1	57	abstract	abstract	VERB
biotropia-204	1	58	the	the	DET
biotropia-204	1	59	aims	aim	NOUN
biotropia-204	1	60	of	of	ADP
biotropia-204	1	61	this	this	DET
biotropia-204	1	62	experiment	experiment	NOUN
biotropia-204	1	63	were	be	AUX
biotropia-204	1	64	to	to	PART
biotropia-204	1	65	isolate	isolate	VERB
biotropia-204	1	66	alkaline	alkaline	NOUN
biotropia-204	1	67	protease	protease	NOUN
biotropia-204	1	68	producing	produce	VERB
biotropia-204	1	69	bacteria	bacteria	NOUN
biotropia-204	1	70	from	from	ADP
biotropia-204	1	71	leather	leather	NOUN
biotropia-204	1	72	processing	processing	NOUN
biotropia-204	1	73	waste	waste	NOUN
biotropia-204	1	74	,	,	PUNCT
biotropia-204	1	75	and	and	CCONJ
biotropia-204	1	76	to	to	PART
biotropia-204	1	77	study	study	VERB
biotropia-204	1	78	the	the	DET
biotropia-204	1	79	biochemical	biochemical	ADJ
biotropia-204	1	80	properties	property	NOUN
biotropia-204	1	81	of	of	ADP
biotropia-204	1	82	the	the	DET
biotropia-204	1	83	enzyme	enzyme	NOUN
biotropia-204	1	84	produced	produce	VERB
biotropia-204	1	85	by	by	ADP
biotropia-204	1	86	the	the	DET
biotropia-204	1	87	selected	select	VERB
biotropia-204	1	88	bacteria	bacteria	NOUN
biotropia-204	1	89	.	.	PUNCT
biotropia-204	2	1	nine	nine	NUM
biotropia-204	2	2	bacterial	bacterial	ADJ
biotropia-204	2	3	isolates	isolate	NOUN
biotropia-204	2	4	incubated	incubate	VERB
biotropia-204	2	5	at	at	ADP
biotropia-204	2	6	37"c	37"c	NUM
biotropia-204	2	7	,	,	PUNCT
biotropia-204	2	8	revealed	reveal	VERB
biotropia-204	2	9	proteolytic	proteolytic	ADJ
biotropia-204	2	10	activity	activity	NOUN
biotropia-204	2	11	on	on	ADP
biotropia-204	2	12	skim	skim	NOUN
biotropia-204	2	13	milk	milk	NOUN
biotropia-204	2	14	containing	contain	VERB
biotropia-204	2	15	media	medium	NOUN
biotropia-204	2	16	.	.	PUNCT
biotropia-204	3	1	four	four	NUM
biotropia-204	3	2	isolates	isolate	NOUN
biotropia-204	3	3	were	be	AUX
biotropia-204	3	4	grown	grow	VERB
biotropia-204	3	5	at	at	ADP
biotropia-204	3	6	ph	ph	PROPN
biotropia-204	3	7	9	9	NUM
biotropia-204	3	8	and	and	CCONJ
biotropia-204	3	9	another	another	DET
biotropia-204	3	10	four	four	NUM
biotropia-204	3	11	isolates	isolate	NOUN
biotropia-204	3	12	at	at	ADP
biotropia-204	3	13	ph	ph	PROPN
biotropia-204	3	14	10	10	NUM
biotropia-204	3	15	and	and	CCONJ
biotropia-204	3	16	only	only	ADV
biotropia-204	3	17	one	one	NUM
biotropia-204	3	18	isolate	isolate	NOUN
biotropia-204	3	19	at	at	ADP
biotropia-204	3	20	ph	ph	PROPN
biotropia-204	3	21	11	11	NUM
biotropia-204	3	22	.	.	PUNCT
biotropia-204	4	1	however	however	ADV
biotropia-204	4	2	,	,	PUNCT
biotropia-204	4	3	in	in	ADP
biotropia-204	4	4	further	further	ADJ
biotropia-204	4	5	subculture	subculture	NOUN
biotropia-204	4	6	,	,	PUNCT
biotropia-204	4	7	there	there	PRON
biotropia-204	4	8	were	be	VERB
biotropia-204	4	9	only	only	ADV
biotropia-204	4	10	three	three	NUM
biotropia-204	4	11	isolates	isolate	NOUN
biotropia-204	4	12	that	that	PRON
biotropia-204	4	13	showed	show	VERB
biotropia-204	4	14	proteolytic	proteolytic	ADJ
biotropia-204	4	15	activity	activity	NOUN
biotropia-204	4	16	,	,	PUNCT
biotropia-204	4	17	namely	namely	ADV
biotropia-204	4	18	,	,	PUNCT
biotropia-204	4	19	d2	d2	PROPN
biotropia-204	4	20	,	,	PUNCT
biotropia-204	4	21	d7	d7	PROPN
biotropia-204	4	22	,	,	PUNCT
biotropia-204	4	23	and	and	CCONJ
biotropia-204	4	24	dl	dl	PROPN
biotropia-204	4	25	l	l	PROPN
biotropia-204	4	26	.	.	PUNCT
biotropia-204	5	1	among	among	ADP
biotropia-204	5	2	the	the	DET
biotropia-204	5	3	three	three	NUM
biotropia-204	5	4	isolates	isolate	NOUN
biotropia-204	5	5	,	,	PUNCT
biotropia-204	5	6	isolate	isolate	PROPN
biotropia-204	5	7	d2	d2	PROPN
biotropia-204	5	8	was	be	AUX
biotropia-204	5	9	the	the	DET
biotropia-204	5	10	highest	high	ADJ
biotropia-204	5	11	protease	protease	NOUN
biotropia-204	5	12	producer	producer	NOUN
biotropia-204	5	13	.	.	PUNCT
biotropia-204	6	1	the	the	DET
biotropia-204	6	2	highest	high	ADJ
biotropia-204	6	3	protease	protease	NOUN
biotropia-204	6	4	production	production	NOUN
biotropia-204	6	5	(	(	PUNCT
biotropia-204	6	6	36.5u	36.5u	NUM
biotropia-204	6	7	/	/	SYM
biotropia-204	6	8	l	l	NOUN
biotropia-204	6	9	)	)	PUNCT
biotropia-204	6	10	was	be	AUX
biotropia-204	6	11	reached	reach	VERB
biotropia-204	6	12	after	after	ADP
biotropia-204	6	13	a	a	DET
biotropia-204	6	14	36	36	NUM
biotropia-204	6	15	-	-	PUNCT
biotropia-204	6	16	hr	hr	NOUN
biotropia-204	6	17	fermentation	fermentation	NOUN
biotropia-204	6	18	at	at	ADP
biotropia-204	6	19	ph	ph	ADJ
biotropia-204	6	20	9	9	NUM
biotropia-204	6	21	.	.	PUNCT
biotropia-204	7	1	the	the	DET
biotropia-204	7	2	optimum	optimum	ADJ
biotropia-204	7	3	activity	activity	NOUN
biotropia-204	7	4	of	of	ADP
biotropia-204	7	5	d2	d2	NOUN
biotropia-204	7	6	protease	protease	NOUN
biotropia-204	7	7	was	be	AUX
biotropia-204	7	8	observed	observe	VERB
biotropia-204	7	9	at	at	ADP
biotropia-204	7	10	ph	ph	PROPN
biotropia-204	7	11	8	8	NUM
biotropia-204	7	12	and	and	CCONJ
biotropia-204	7	13	60"c	60"c	NUM
biotropia-204	7	14	.	.	PUNCT
biotropia-204	8	1	the	the	DET
biotropia-204	8	2	enzyme	enzyme	NOUN
biotropia-204	8	3	was	be	AUX
biotropia-204	8	4	stable	stable	ADJ
biotropia-204	8	5	at	at	ADP
biotropia-204	8	6	ph	ph	ADJ
biotropia-204	8	7	range	range	NOUN
biotropia-204	8	8	of	of	ADP
biotropia-204	8	9	7	7	NUM
biotropia-204	8	10	-	-	SYM
biotropia-204	8	11	10	10	NUM
biotropia-204	8	12	,	,	PUNCT
biotropia-204	8	13	and	and	CCONJ
biotropia-204	8	14	at	at	ADP
biotropia-204	8	15	temperature	temperature	NOUN
biotropia-204	8	16	of	of	ADP
biotropia-204	8	17	52	52	NUM
biotropia-204	8	18	-	-	SYM
biotropia-204	8	19	62"c	62"c	PROPN
biotropia-204	8	20	.	.	PUNCT
biotropia-204	9	1	in	in	ADP
biotropia-204	9	2	the	the	DET
biotropia-204	9	3	presence	presence	NOUN
biotropia-204	9	4	of	of	ADP
biotropia-204	9	5	5	5	NUM
biotropia-204	9	6	mm	mm	NOUN
biotropia-204	9	7	edta	edta	NOUN
biotropia-204	9	8	or	or	CCONJ
biotropia-204	9	9	pmsf	pmsf	NOUN
biotropia-204	9	10	,	,	PUNCT
biotropia-204	9	11	the	the	DET
biotropia-204	9	12	crude	crude	ADJ
biotropia-204	9	13	enzyme	enzyme	NOUN
biotropia-204	9	14	activity	activity	NOUN
biotropia-204	9	15	decreased	decrease	VERB
biotropia-204	9	16	to	to	ADP
biotropia-204	9	17	7.04	7.04	NUM
biotropia-204	9	18	%	%	NOUN
biotropia-204	9	19	and	and	CCONJ
biotropia-204	9	20	23.29	23.29	NUM
biotropia-204	9	21	%	%	NOUN
biotropia-204	9	22	respectively	respectively	ADV
biotropia-204	9	23	,	,	PUNCT
biotropia-204	9	24	which	which	PRON
biotropia-204	9	25	indicated	indicate	VERB
biotropia-204	9	26	that	that	SCONJ
biotropia-204	9	27	the	the	DET
biotropia-204	9	28	enzyme	enzyme	NOUN
biotropia-204	9	29	might	might	AUX
biotropia-204	9	30	be	be	AUX
biotropia-204	9	31	a	a	DET
biotropia-204	9	32	metal	metal	NOUN
biotropia-204	9	33	dependent	dependent	ADJ
biotropia-204	9	34	serine	serine	NOUN
biotropia-204	9	35	protease	protease	NOUN
biotropia-204	9	36	.	.	PUNCT
biotropia-204	10	1	zymogram	zymogram	NOUN
biotropia-204	10	2	analysis	analysis	NOUN
biotropia-204	10	3	revealed	reveal	VERB
biotropia-204	10	4	the	the	DET
biotropia-204	10	5	molecular	molecular	ADJ
biotropia-204	10	6	weight	weight	NOUN
biotropia-204	10	7	of	of	ADP
biotropia-204	10	8	the	the	DET
biotropia-204	10	9	enzyme	enzyme	NOUN
biotropia-204	10	10	was	be	AUX
biotropia-204	10	11	about	about	ADV
biotropia-204	10	12	42.8kd	42.8kd	NUM
biotropia-204	10	13	.	.	PUNCT
biotropia-204	11	1	keywords	keyword	NOUN
biotropia-204	11	2	:	:	PUNCT
biotropia-204	11	3	leather/	leather/	NUM
biotropia-204	11	4	waste	waste	NOUN
biotropia-204	11	5	/	/	SYM
biotropia-204	11	6	protease	protease	NOUN
biotropia-204	11	7	/	/	SYM
biotropia-204	11	8	alkaline	alkaline	NOUN
biotropia-204	11	9	introduction	introduction	NOUN
biotropia-204	11	10	alkaline	alkaline	NOUN
biotropia-204	11	11	protease	protease	NOUN
biotropia-204	11	12	,	,	PUNCT
biotropia-204	11	13	along	along	ADP
biotropia-204	11	14	with	with	ADP
biotropia-204	11	15	other	other	ADJ
biotropia-204	11	16	alkaline	alkaline	NOUN
biotropia-204	11	17	enzymes	enzyme	NOUN
biotropia-204	11	18	such	such	ADJ
biotropia-204	11	19	as	as	ADP
biotropia-204	11	20	amylase	amylase	NOUN
biotropia-204	11	21	and	and	CCONJ
biotropia-204	11	22	cellulose	cellulose	NOUN
biotropia-204	11	23	,	,	PUNCT
biotropia-204	11	24	has	have	AUX
biotropia-204	11	25	been	be	AUX
biotropia-204	11	26	used	use	VERB
biotropia-204	11	27	on	on	ADP
biotropia-204	11	28	an	an	DET
biotropia-204	11	29	industrial	industrial	ADJ
biotropia-204	11	30	scale	scale	NOUN
biotropia-204	11	31	.	.	PUNCT
biotropia-204	12	1	microbial	microbial	ADJ
biotropia-204	12	2	akaline	akaline	NOUN
biotropia-204	12	3	protease	protease	NOUN
biotropia-204	12	4	is	be	AUX
biotropia-204	12	5	the	the	DET
biotropia-204	12	6	enzyme	enzyme	NOUN
biotropia-204	12	7	that	that	PRON
biotropia-204	12	8	dominate	dominate	VERB
biotropia-204	12	9	commercial	commercial	ADJ
biotropia-204	12	10	application	application	NOUN
biotropia-204	12	11	,	,	PUNCT
biotropia-204	12	12	mainly	mainly	ADV
biotropia-204	12	13	for	for	ADP
biotropia-204	12	14	laundry	laundry	ADJ
biotropia-204	12	15	detergents	detergent	NOUN
biotropia-204	12	16	.	.	PUNCT
biotropia-204	13	1	alkaline	alkaline	ADJ
biotropia-204	13	2	protease	protease	NOUN
biotropia-204	13	3	with	with	ADP
biotropia-204	13	4	elastolytic	elastolytic	ADJ
biotropia-204	13	5	and	and	CCONJ
biotropia-204	13	6	keratolytic	keratolytic	ADJ
biotropia-204	13	7	activity	activity	NOUN
biotropia-204	13	8	is	be	AUX
biotropia-204	13	9	used	use	VERB
biotropia-204	13	10	in	in	ADP
biotropia-204	13	11	the	the	DET
biotropia-204	13	12	tannery	tannery	NOUN
biotropia-204	13	13	industry	industry	NOUN
biotropia-204	13	14	for	for	ADP
biotropia-204	13	15	dehairing	dehaire	VERB
biotropia-204	13	16	and	and	CCONJ
biotropia-204	13	17	the	the	DET
biotropia-204	13	18	bating	bating	NOUN
biotropia-204	13	19	of	of	ADP
biotropia-204	13	20	skin	skin	NOUN
biotropia-204	13	21	and	and	CCONJ
biotropia-204	13	22	hides	hide	NOUN
biotropia-204	13	23	(	(	PUNCT
biotropia-204	13	24	taylor	taylor	PROPN
biotropia-204	13	25	et	et	PROPN
biotropia-204	13	26	al	al	PROPN
biotropia-204	13	27	.	.	PROPN
biotropia-204	13	28	1987	1987	NUM
biotropia-204	13	29	)	)	PUNCT
biotropia-204	13	30	.	.	PUNCT
biotropia-204	14	1	alkaline	alkaline	NOUN
biotropia-204	14	2	protease	protease	NOUN
biotropia-204	14	3	from	from	ADP
biotropia-204	14	4	bacillus	bacillus	NOUN
biotropia-204	14	5	spp	spp	NOUN
biotropia-204	14	6	.	.	PUNCT
biotropia-204	14	7	was	be	AUX
biotropia-204	14	8	reported	report	VERB
biotropia-204	14	9	to	to	PART
biotropia-204	14	10	be	be	AUX
biotropia-204	14	11	used	use	VERB
biotropia-204	14	12	to	to	PART
biotropia-204	14	13	decompose	decompose	VERB
biotropia-204	14	14	gelatin	gelatin	NOUN
biotropia-204	14	15	on	on	ADP
biotropia-204	14	16	x	x	ADJ
biotropia-204	14	17	-	-	NOUN
biotropia-204	14	18	ray	ray	NOUN
biotropia-204	14	19	film	film	NOUN
biotropia-204	14	20	for	for	ADP
biotropia-204	14	21	silver	silver	NOUN
biotropia-204	14	22	recovery	recovery	NOUN
biotropia-204	14	23	(	(	PUNCT
biotropia-204	14	24	horikoshi	horikoshi	PROPN
biotropia-204	14	25	1996	1996	NUM
biotropia-204	14	26	)	)	PUNCT
biotropia-204	14	27	.	.	PUNCT
biotropia-204	15	1	other	other	ADJ
biotropia-204	15	2	uses	use	NOUN
biotropia-204	15	3	of	of	ADP
biotropia-204	15	4	alkaline	alkaline	NOUN
biotropia-204	15	5	protease	protease	NOUN
biotropia-204	15	6	are	be	AUX
biotropia-204	15	7	for	for	ADP
biotropia-204	15	8	medical	medical	ADJ
biotropia-204	15	9	purposes	purpose	NOUN
biotropia-204	15	10	,	,	PUNCT
biotropia-204	15	11	food	food	NOUN
biotropia-204	15	12	and	and	CCONJ
biotropia-204	15	13	chemical	chemical	NOUN
biotropia-204	15	14	industries	industry	NOUN
biotropia-204	15	15	,	,	PUNCT
biotropia-204	15	16	as	as	ADV
biotropia-204	15	17	well	well	ADV
biotropia-204	15	18	as	as	ADP
biotropia-204	15	19	in	in	ADP
biotropia-204	15	20	waste	waste	NOUN
biotropia-204	15	21	treatment	treatment	NOUN
biotropia-204	15	22	plants	plant	NOUN
biotropia-204	15	23	.	.	PUNCT
biotropia-204	16	1	alkaline	alkaline	NOUN
biotropia-204	16	2	protease	protease	NOUN
biotropia-204	16	3	has	have	AUX
biotropia-204	16	4	been	be	AUX
biotropia-204	16	5	used	use	VERB
biotropia-204	16	6	in	in	ADP
biotropia-204	16	7	industrial	industrial	ADJ
biotropia-204	16	8	scales	scale	NOUN
biotropia-204	16	9	and	and	CCONJ
biotropia-204	16	10	has	have	AUX
biotropia-204	16	11	been	be	AUX
biotropia-204	16	12	an	an	DET
biotropia-204	16	13	attractive	attractive	ADJ
biotropia-204	16	14	option	option	NOUN
biotropia-204	16	15	for	for	ADP
biotropia-204	16	16	researchers	researcher	NOUN
biotropia-204	16	17	for	for	ADP
biotropia-204	16	18	the	the	DET
biotropia-204	16	19	last	last	ADJ
biotropia-204	16	20	three	three	NUM
biotropia-204	16	21	decades	decade	NOUN
biotropia-204	16	22	since	since	SCONJ
biotropia-204	16	23	the	the	DET
biotropia-204	16	24	first	first	ADJ
biotropia-204	16	25	publication	publication	NOUN
biotropia-204	16	26	concerning	concern	VERB
biotropia-204	16	27	the	the	DET
biotropia-204	16	28	enzyme	enzyme	NOUN
biotropia-204	16	29	by	by	ADP
biotropia-204	16	30	horikoshi	horikoshi	PROPN
biotropia-204	16	31	(	(	PUNCT
biotropia-204	16	32	1971	1971	NUM
biotropia-204	16	33	)	)	PUNCT
biotropia-204	16	34	.	.	PUNCT
biotropia-204	17	1	studies	study	NOUN
biotropia-204	17	2	on	on	ADP
biotropia-204	17	3	exploration	exploration	NOUN
biotropia-204	17	4	of	of	ADP
biotropia-204	17	5	alkaline	alkaline	NOUN
biotropia-204	17	6	protease	protease	NOUN
biotropia-204	17	7	producing	produce	VERB
biotropia-204	17	8	microorganisms	microorganism	NOUN
biotropia-204	17	9	,	,	PUNCT
biotropia-204	17	10	which	which	PRON
biotropia-204	17	11	produce	produce	VERB
biotropia-204	17	12	novel	novel	ADJ
biotropia-204	17	13	enzymes	enzyme	NOUN
biotropia-204	17	14	have	have	AUX
biotropia-204	17	15	been	be	AUX
biotropia-204	17	16	extensively	extensively	ADV
biotropia-204	17	17	carried	carry	VERB
biotropia-204	17	18	out	out	ADP
biotropia-204	17	19	by	by	ADP
biotropia-204	17	20	researchers	researcher	NOUN
biotropia-204	17	21	all	all	ADV
biotropia-204	17	22	over	over	ADP
biotropia-204	17	23	the	the	DET
biotropia-204	17	24	world	world	NOUN
biotropia-204	17	25	(	(	PUNCT
biotropia-204	17	26	banerjee	banerjee	PROPN
biotropia-204	17	27	et	et	PROPN
biotropia-204	17	28	al	al	PROPN
biotropia-204	17	29	.	.	PROPN
biotropia-204	17	30	1999	1999	NUM
biotropia-204	17	31	;	;	PUNCT
biotropia-204	17	32	singh	singh	PROPN
biotropia-204	17	33	et	et	PROPN
biotropia-204	17	34	al	al	PROPN
biotropia-204	17	35	.	.	PROPN
biotropia-204	17	36	2001	2001	NUM
biotropia-204	17	37	;	;	PUNCT
biotropia-204	17	38	johnvesly	johnvesly	ADV
biotropia-204	17	39	&	&	CCONJ
biotropia-204	17	40	naik	naik	PROPN
biotropia-204	17	41	,	,	PUNCT
biotropia-204	17	42	2001	2001	NUM
biotropia-204	17	43	;	;	PUNCT
biotropia-204	17	44	kanekar	kanekar	PROPN
biotropia-204	17	45	et	et	PROPN
biotropia-204	17	46	al	al	PROPN
biotropia-204	17	47	.	.	PROPN
biotropia-204	17	48	2002	2002	NUM
biotropia-204	17	49	,	,	PUNCT
biotropia-204	17	50	joo	joo	PROPN
biotropia-204	17	51	et	et	PROPN
biotropia-204	17	52	al	al	PROPN
biotropia-204	17	53	2002	2002	NUM
biotropia-204	17	54	;	;	PUNCT
biotropia-204	17	55	gessesse	gessesse	VERB
biotropia-204	17	56	et	et	PROPN
biotropia-204	17	57	al	al	PROPN
biotropia-204	17	58	.	.	PROPN
biotropia-204	17	59	2002	2002	NUM
biotropia-204	17	60	;	;	PUNCT
biotropia-204	17	61	moreira	moreira	PROPN
biotropia-204	17	62	et	et	PROPN
biotropia-204	17	63	al	al	PROPN
biotropia-204	17	64	.	.	PROPN
biotropia-204	17	65	2003	2003	NUM
biotropia-204	17	66	)	)	PUNCT
biotropia-204	17	67	.	.	PUNCT
biotropia-204	18	1	29	29	NUM
biotropia-204	18	2	biotropia	biotropia	NOUN
biotropia-204	18	3	no	no	NOUN
biotropia-204	18	4	.	.	PROPN
biotropia-204	18	5	22	22	NUM
biotropia-204	18	6	,	,	PUNCT
biotropia-204	18	7	2004	2004	NUM
biotropia-204	18	8	most	most	ADJ
biotropia-204	18	9	of	of	ADP
biotropia-204	18	10	the	the	DET
biotropia-204	18	11	alkaline	alkaline	NOUN
biotropia-204	18	12	protease	protease	NOUN
biotropia-204	18	13	producing	produce	VERB
biotropia-204	18	14	microorganisms	microorganism	NOUN
biotropia-204	18	15	were	be	AUX
biotropia-204	18	16	isolated	isolate	VERB
biotropia-204	18	17	from	from	ADP
biotropia-204	18	18	natural	natural	ADJ
biotropia-204	18	19	,	,	PUNCT
biotropia-204	18	20	as	as	ADV
biotropia-204	18	21	well	well	ADV
biotropia-204	18	22	as	as	ADP
biotropia-204	18	23	manmade	manmade	NOUN
biotropia-204	18	24	alkaline	alkaline	NOUN
biotropia-204	18	25	environments	environment	NOUN
biotropia-204	18	26	.	.	PUNCT
biotropia-204	19	1	such	such	ADJ
biotropia-204	19	2	microorganisms	microorganism	NOUN
biotropia-204	19	3	were	be	AUX
biotropia-204	19	4	also	also	ADV
biotropia-204	19	5	isolated	isolate	VERB
biotropia-204	19	6	from	from	ADP
biotropia-204	19	7	non	non	ADJ
biotropia-204	19	8	-	-	ADJ
biotropia-204	19	9	alkaline	alkaline	ADJ
biotropia-204	19	10	habitats	habitat	NOUN
biotropia-204	19	11	,	,	PUNCT
biotropia-204	19	12	such	such	ADJ
biotropia-204	19	13	as	as	ADP
biotropia-204	19	14	neutral	neutral	ADJ
biotropia-204	19	15	,	,	PUNCT
biotropia-204	19	16	and	and	CCONJ
biotropia-204	19	17	acidic	acidic	ADJ
biotropia-204	19	18	soil	soil	NOUN
biotropia-204	19	19	(	(	PUNCT
biotropia-204	19	20	kumar	kumar	PROPN
biotropia-204	19	21	et	et	PROPN
biotropia-204	19	22	al	al	PROPN
biotropia-204	19	23	.	.	PROPN
biotropia-204	19	24	1999	1999	NUM
biotropia-204	19	25	)	)	PUNCT
biotropia-204	19	26	.	.	PUNCT
biotropia-204	20	1	this	this	DET
biotropia-204	20	2	experiment	experiment	NOUN
biotropia-204	20	3	aims	aim	VERB
biotropia-204	20	4	to	to	PART
biotropia-204	20	5	isolate	isolate	VERB
biotropia-204	20	6	alkaline	alkaline	NOUN
biotropia-204	20	7	protease	protease	NOUN
biotropia-204	20	8	producing	produce	VERB
biotropia-204	20	9	microorganisms	microorganism	NOUN
biotropia-204	20	10	from	from	ADP
biotropia-204	20	11	alkaline	alkaline	ADJ
biotropia-204	20	12	waste	waste	NOUN
biotropia-204	20	13	of	of	ADP
biotropia-204	20	14	leather	leather	NOUN
biotropia-204	20	15	processing	processing	NOUN
biotropia-204	20	16	and	and	CCONJ
biotropia-204	20	17	to	to	PART
biotropia-204	20	18	study	study	VERB
biotropia-204	20	19	the	the	DET
biotropia-204	20	20	biochemical	biochemical	ADJ
biotropia-204	20	21	properties	property	NOUN
biotropia-204	20	22	of	of	ADP
biotropia-204	20	23	the	the	DET
biotropia-204	20	24	enzyme	enzyme	NOUN
biotropia-204	20	25	of	of	ADP
biotropia-204	20	26	the	the	DET
biotropia-204	20	27	selected	select	VERB
biotropia-204	20	28	isolate	isolate	NOUN
biotropia-204	20	29	.	.	PUNCT
biotropia-204	21	1	materials	material	NOUN
biotropia-204	21	2	and	and	CCONJ
biotropia-204	21	3	methods	method	NOUN
biotropia-204	21	4	isolation	isolation	NOUN
biotropia-204	21	5	,	,	PUNCT
biotropia-204	21	6	screening	screening	NOUN
biotropia-204	21	7	and	and	CCONJ
biotropia-204	21	8	identification	identification	NOUN
biotropia-204	21	9	of	of	ADP
biotropia-204	21	10	microbial	microbial	ADJ
biotropia-204	21	11	strains	strain	VERB
biotropia-204	21	12	the	the	DET
biotropia-204	21	13	samples	sample	NOUN
biotropia-204	21	14	for	for	ADP
biotropia-204	21	15	screening	screening	NOUN
biotropia-204	21	16	were	be	AUX
biotropia-204	21	17	taken	take	VERB
biotropia-204	21	18	from	from	ADP
biotropia-204	21	19	the	the	DET
biotropia-204	21	20	waste	waste	NOUN
biotropia-204	21	21	of	of	ADP
biotropia-204	21	22	different	different	ADJ
biotropia-204	21	23	leather	leather	NOUN
biotropia-204	21	24	processing	processing	NOUN
biotropia-204	21	25	steps	step	NOUN
biotropia-204	21	26	including	include	VERB
biotropia-204	21	27	soaking	soak	VERB
biotropia-204	21	28	(	(	PUNCT
biotropia-204	21	29	ph	ph	NOUN
biotropia-204	21	30	10	10	NUM
biotropia-204	21	31	)	)	PUNCT
biotropia-204	21	32	,	,	PUNCT
biotropia-204	22	1	liming	liming	NOUN
biotropia-204	22	2	(	(	PUNCT
biotropia-204	22	3	ph	ph	NOUN
biotropia-204	22	4	13	13	NUM
biotropia-204	22	5	)	)	PUNCT
biotropia-204	22	6	,	,	PUNCT
biotropia-204	22	7	and	and	CCONJ
biotropia-204	22	8	deliming	delime	VERB
biotropia-204	22	9	(	(	PUNCT
biotropia-204	22	10	ph	ph	NOUN
biotropia-204	22	11	8.5	8.5	NUM
biotropia-204	22	12	-	-	SYM
biotropia-204	22	13	9.0	9.0	NUM
biotropia-204	22	14	)	)	PUNCT
biotropia-204	22	15	from	from	ADP
biotropia-204	22	16	pt	pt	PROPN
biotropia-204	22	17	.	.	PROPN
biotropia-204	22	18	muhara	muhara	PROPN
biotropia-204	22	19	dwi	dwi	PROPN
biotropia-204	22	20	tunggal	tunggal	PROPN
biotropia-204	22	21	laju	laju	PROPN
biotropia-204	22	22	,	,	PUNCT
biotropia-204	22	23	and	and	CCONJ
biotropia-204	22	24	pt	pt	PROPN
biotropia-204	22	25	.	.	PROPN
biotropia-204	22	26	gunung	gunung	PROPN
biotropia-204	22	27	putri	putri	PROPN
biotropia-204	22	28	,	,	PUNCT
biotropia-204	22	29	in	in	ADP
biotropia-204	22	30	bogor	bogor	NOUN
biotropia-204	22	31	.	.	PUNCT
biotropia-204	23	1	the	the	DET
biotropia-204	23	2	microorganisms	microorganism	NOUN
biotropia-204	23	3	were	be	AUX
biotropia-204	23	4	enriched	enrich	VERB
biotropia-204	23	5	on	on	ADP
biotropia-204	23	6	agar	agar	NOUN
biotropia-204	23	7	plates	plate	NOUN
biotropia-204	23	8	containing	contain	VERB
biotropia-204	23	9	(	(	PUNCT
biotropia-204	23	10	gram	gram	NOUN
biotropia-204	23	11	per	per	ADP
biotropia-204	23	12	liter	liter	NOUN
biotropia-204	23	13	)	)	PUNCT
biotropia-204	23	14	2	2	NUM
biotropia-204	23	15	gr	gr	NOUN
biotropia-204	23	16	yeast	yeast	NOUN
biotropia-204	23	17	extract	extract	NOUN
biotropia-204	23	18	,	,	PUNCT
biotropia-204	23	19	0.4	0.4	NUM
biotropia-204	23	20	gr	gr	NOUN
biotropia-204	23	21	(	(	PUNCT
biotropia-204	23	22	nh4)2so4	nh4)2so4	PROPN
biotropia-204	23	23	,	,	PUNCT
biotropia-204	23	24	1	1	NUM
biotropia-204	23	25	gr	gr	ADP
biotropia-204	23	26	mgso4.7h2o	mgso4.7h2o	PROPN
biotropia-204	23	27	,	,	PUNCT
biotropia-204	23	28	0.5	0.5	NUM
biotropia-204	23	29	gr	gr	NUM
biotropia-204	23	30	cacl^.2	cacl^.2	PROPN
biotropia-204	23	31	h2o	h2o	PROPN
biotropia-204	23	32	,	,	PUNCT
biotropia-204	23	33	0.8	0.8	NUM
biotropia-204	23	34	gr	gr	NUM
biotropia-204	23	35	kh2po4	kh2po4	NOUN
biotropia-204	23	36	,	,	PUNCT
biotropia-204	23	37	40	40	NUM
biotropia-204	23	38	gr	gr	NUM
biotropia-204	23	39	agar	agar	NOUN
biotropia-204	23	40	,	,	PUNCT
biotropia-204	23	41	and	and	CCONJ
biotropia-204	23	42	8	8	NUM
biotropia-204	23	43	gr	gr	NOUN
biotropia-204	23	44	skimmed	skim	VERB
biotropia-204	23	45	milk	milk	NOUN
biotropia-204	23	46	.	.	PUNCT
biotropia-204	24	1	the	the	DET
biotropia-204	24	2	ph	ph	PROPN
biotropia-204	24	3	medium	medium	NOUN
biotropia-204	24	4	was	be	AUX
biotropia-204	24	5	adjusted	adjust	VERB
biotropia-204	24	6	to	to	ADP
biotropia-204	24	7	8	8	NUM
biotropia-204	24	8	-	-	SYM
biotropia-204	24	9	11	11	NUM
biotropia-204	24	10	using	use	VERB
biotropia-204	24	11	naoh	naoh	NOUN
biotropia-204	24	12	.	.	PUNCT
biotropia-204	25	1	the	the	DET
biotropia-204	25	2	microorganisms	microorganism	NOUN
biotropia-204	25	3	were	be	AUX
biotropia-204	25	4	incubated	incubate	VERB
biotropia-204	25	5	at	at	ADP
biotropia-204	25	6	24	24	NUM
biotropia-204	25	7	-	-	SYM
biotropia-204	25	8	40	40	NUM
biotropia-204	25	9	°	°	NUM
biotropia-204	25	10	c	c	NOUN
biotropia-204	25	11	for	for	ADP
biotropia-204	25	12	3	3	NUM
biotropia-204	25	13	days	day	NOUN
biotropia-204	25	14	.	.	PUNCT
biotropia-204	26	1	the	the	DET
biotropia-204	26	2	colonies	colony	NOUN
biotropia-204	26	3	which	which	PRON
biotropia-204	26	4	revealed	reveal	VERB
biotropia-204	26	5	proteolytic	proteolytic	ADJ
biotropia-204	26	6	activity	activity	NOUN
biotropia-204	26	7	were	be	AUX
biotropia-204	26	8	then	then	ADV
biotropia-204	26	9	isolated	isolate	VERB
biotropia-204	26	10	and	and	CCONJ
biotropia-204	26	11	screened	screen	VERB
biotropia-204	26	12	further	far	ADV
biotropia-204	26	13	,	,	PUNCT
biotropia-204	26	14	using	use	VERB
biotropia-204	26	15	the	the	DET
biotropia-204	26	16	same	same	ADJ
biotropia-204	26	17	composition	composition	NOUN
biotropia-204	26	18	of	of	ADP
biotropia-204	26	19	medium	medium	NOUN
biotropia-204	26	20	.	.	PUNCT
biotropia-204	27	1	the	the	DET
biotropia-204	27	2	isolates	isolate	NOUN
biotropia-204	27	3	were	be	AUX
biotropia-204	27	4	screened	screen	VERB
biotropia-204	27	5	based	base	VERB
biotropia-204	27	6	on	on	ADP
biotropia-204	27	7	proteolytic	proteolytic	ADJ
biotropia-204	27	8	index	index	NOUN
biotropia-204	27	9	which	which	PRON
biotropia-204	27	10	defined	define	VERB
biotropia-204	27	11	as	as	ADP
biotropia-204	27	12	a	a	DET
biotropia-204	27	13	ratio	ratio	NOUN
biotropia-204	27	14	of	of	ADP
biotropia-204	27	15	clear	clear	ADJ
biotropia-204	27	16	zone	zone	NOUN
biotropia-204	27	17	and	and	CCONJ
biotropia-204	27	18	colony	colony	NOUN
biotropia-204	27	19	diameter	diameter	NOUN
biotropia-204	27	20	.	.	PUNCT
biotropia-204	28	1	the	the	DET
biotropia-204	28	2	isolates	isolate	NOUN
biotropia-204	28	3	that	that	PRON
biotropia-204	28	4	had	have	VERB
biotropia-204	28	5	the	the	DET
biotropia-204	28	6	highest	high	ADJ
biotropia-204	28	7	proteolytic	proteolytic	ADJ
biotropia-204	28	8	index	index	NOUN
biotropia-204	28	9	were	be	AUX
biotropia-204	28	10	screened	screen	VERB
biotropia-204	28	11	further	far	ADV
biotropia-204	28	12	for	for	SCONJ
biotropia-204	28	13	protease	protease	NOUN
biotropia-204	28	14	production	production	NOUN
biotropia-204	28	15	in	in	ADP
biotropia-204	28	16	luria	luria	PROPN
biotropia-204	28	17	broth	broth	NOUN
biotropia-204	28	18	containing	contain	VERB
biotropia-204	28	19	1	1	NUM
biotropia-204	28	20	%	%	NOUN
biotropia-204	28	21	peptone	peptone	NOUN
biotropia-204	28	22	,	,	PUNCT
biotropia-204	28	23	0.5	0.5	NUM
biotropia-204	28	24	gr	gr	NOUN
biotropia-204	28	25	yeast	yeast	NOUN
biotropia-204	28	26	extract	extract	NOUN
biotropia-204	28	27	and	and	CCONJ
biotropia-204	28	28	0.5	0.5	NUM
biotropia-204	28	29	%	%	NOUN
biotropia-204	28	30	nacl	nacl	NOUN
biotropia-204	28	31	at	at	ADP
biotropia-204	28	32	ph	ph	ADJ
biotropia-204	28	33	9	9	NUM
biotropia-204	28	34	,	,	PUNCT
biotropia-204	28	35	10	10	NUM
biotropia-204	28	36	and	and	CCONJ
biotropia-204	28	37	11	11	NUM
biotropia-204	28	38	.	.	PUNCT
biotropia-204	29	1	in	in	ADP
biotropia-204	29	2	the	the	DET
biotropia-204	29	3	same	same	ADJ
biotropia-204	29	4	medium	medium	ADJ
biotropia-204	29	5	composition	composition	NOUN
biotropia-204	29	6	,	,	PUNCT
biotropia-204	29	7	they	they	PRON
biotropia-204	29	8	were	be	AUX
biotropia-204	29	9	then	then	ADV
biotropia-204	29	10	incubated	incubate	VERB
biotropia-204	29	11	in	in	ADP
biotropia-204	29	12	a	a	DET
biotropia-204	29	13	shaking	shake	VERB
biotropia-204	29	14	incubator	incubator	NOUN
biotropia-204	29	15	for	for	ADP
biotropia-204	29	16	19	19	NUM
biotropia-204	29	17	hours	hour	NOUN
biotropia-204	29	18	at	at	ADP
biotropia-204	29	19	37	37	NUM
biotropia-204	29	20	°	°	NOUN
biotropia-204	29	21	c	c	NOUN
biotropia-204	29	22	.	.	PUNCT
biotropia-204	30	1	the	the	DET
biotropia-204	30	2	fermented	ferment	VERB
biotropia-204	30	3	broth	broth	NOUN
biotropia-204	30	4	was	be	AUX
biotropia-204	30	5	then	then	ADV
biotropia-204	30	6	centrifuged	centrifuge	VERB
biotropia-204	30	7	at	at	ADP
biotropia-204	30	8	4000	4000	NUM
biotropia-204	30	9	rpm	rpm	NOUN
biotropia-204	30	10	for	for	ADP
biotropia-204	30	11	20	20	NUM
biotropia-204	30	12	minutes	minute	NOUN
biotropia-204	30	13	.	.	PUNCT
biotropia-204	31	1	the	the	DET
biotropia-204	31	2	protease	protease	NOUN
biotropia-204	31	3	activity	activity	NOUN
biotropia-204	31	4	was	be	AUX
biotropia-204	31	5	finally	finally	ADV
biotropia-204	31	6	assayed	assayed	ADJ
biotropia-204	31	7	and	and	CCONJ
biotropia-204	31	8	the	the	DET
biotropia-204	31	9	isolates	isolate	NOUN
biotropia-204	31	10	were	be	AUX
biotropia-204	31	11	selected	select	VERB
biotropia-204	31	12	based	base	VERB
biotropia-204	31	13	on	on	ADP
biotropia-204	31	14	the	the	DET
biotropia-204	31	15	protease	protease	NOUN
biotropia-204	31	16	production	production	NOUN
biotropia-204	31	17	.	.	PUNCT
biotropia-204	32	1	protease	protease	NOUN
biotropia-204	32	2	assay	assay	NOUN
biotropia-204	32	3	protease	protease	NOUN
biotropia-204	32	4	activity	activity	NOUN
biotropia-204	32	5	was	be	AUX
biotropia-204	32	6	measured	measure	VERB
biotropia-204	32	7	using	use	VERB
biotropia-204	32	8	a	a	DET
biotropia-204	32	9	method	method	NOUN
biotropia-204	32	10	suggested	suggest	VERB
biotropia-204	32	11	by	by	ADP
biotropia-204	32	12	walter	walter	PROPN
biotropia-204	32	13	(	(	PUNCT
biotropia-204	32	14	1984	1984	NUM
biotropia-204	32	15	)	)	PUNCT
biotropia-204	32	16	.	.	PUNCT
biotropia-204	33	1	the	the	DET
biotropia-204	33	2	method	method	NOUN
biotropia-204	33	3	was	be	AUX
biotropia-204	33	4	based	base	VERB
biotropia-204	33	5	on	on	ADP
biotropia-204	33	6	amino	amino	NOUN
biotropia-204	33	7	acid	acid	NOUN
biotropia-204	33	8	hydrolyzed	hydrolyze	VERB
biotropia-204	33	9	by	by	ADP
biotropia-204	33	10	the	the	DET
biotropia-204	33	11	enzyme	enzyme	NOUN
biotropia-204	33	12	and	and	CCONJ
biotropia-204	33	13	was	be	AUX
biotropia-204	33	14	calculated	calculate	VERB
biotropia-204	33	15	as	as	ADP
biotropia-204	33	16	tyrosine	tyrosine	NOUN
biotropia-204	33	17	.	.	PUNCT
biotropia-204	34	1	one	one	NUM
biotropia-204	34	2	unit	unit	NOUN
biotropia-204	34	3	of	of	ADP
biotropia-204	34	4	enzyme	enzyme	NOUN
biotropia-204	34	5	activity	activity	NOUN
biotropia-204	34	6	was	be	AUX
biotropia-204	34	7	defined	define	VERB
biotropia-204	34	8	as	as	ADP
biotropia-204	34	9	the	the	DET
biotropia-204	34	10	amount	amount	NOUN
biotropia-204	34	11	of	of	ADP
biotropia-204	34	12	enzyme	enzyme	NOUN
biotropia-204	34	13	which	which	PRON
biotropia-204	34	14	liberate	liberate	VERB
biotropia-204	34	15	one	one	NUM
biotropia-204	34	16	micromol	micromol	NOUN
biotropia-204	34	17	tyrosine	tyrosine	NOUN
biotropia-204	34	18	per	per	ADP
biotropia-204	34	19	minute	minute	NOUN
biotropia-204	34	20	at	at	ADP
biotropia-204	34	21	certain	certain	ADJ
biotropia-204	34	22	condition	condition	NOUN
biotropia-204	34	23	.	.	PUNCT
biotropia-204	35	1	preparation	preparation	NOUN
biotropia-204	35	2	of	of	ADP
biotropia-204	35	3	protease	protease	NOUN
biotropia-204	35	4	the	the	DET
biotropia-204	35	5	selected	select	VERB
biotropia-204	35	6	isolate	isolate	NOUN
biotropia-204	35	7	was	be	AUX
biotropia-204	35	8	cultured	cultured	ADJ
biotropia-204	35	9	in	in	ADP
biotropia-204	35	10	5	5	NUM
biotropia-204	35	11	l	l	NOUN
biotropia-204	35	12	fermentor	fermentor	NOUN
biotropia-204	35	13	containing	contain	VERB
biotropia-204	35	14	2.5	2.5	NUM
biotropia-204	35	15	l	l	NOUN
biotropia-204	35	16	enzyme	enzyme	NOUN
biotropia-204	35	17	media	medium	NOUN
biotropia-204	35	18	production	production	NOUN
biotropia-204	35	19	with	with	ADP
biotropia-204	35	20	the	the	DET
biotropia-204	35	21	same	same	ADJ
biotropia-204	35	22	composition	composition	NOUN
biotropia-204	35	23	as	as	SCONJ
biotropia-204	35	24	mentioned	mention	VERB
biotropia-204	35	25	above	above	ADV
biotropia-204	35	26	with	with	ADP
biotropia-204	35	27	addition	addition	NOUN
biotropia-204	35	28	of	of	ADP
biotropia-204	35	29	0.2	0.2	NUM
biotropia-204	35	30	%	%	NOUN
biotropia-204	35	31	skim	skim	NOUN
biotropia-204	35	32	milk	milk	NOUN
biotropia-204	35	33	as	as	ADP
biotropia-204	35	34	an	an	DET
biotropia-204	35	35	inducer	inducer	NOUN
biotropia-204	35	36	.	.	PUNCT
biotropia-204	36	1	after	after	ADP
biotropia-204	36	2	a	a	DET
biotropia-204	36	3	36	36	NUM
biotropia-204	36	4	-	-	PUNCT
biotropia-204	36	5	hr	hr	NOUN
biotropia-204	36	6	incubation	incubation	NOUN
biotropia-204	36	7	at	at	ADP
biotropia-204	36	8	37	37	NUM
biotropia-204	36	9	°	°	NOUN
biotropia-204	36	10	c	c	NOUN
biotropia-204	36	11	,	,	PUNCT
biotropia-204	36	12	the	the	DET
biotropia-204	36	13	fermented	ferment	VERB
biotropia-204	36	14	broth	broth	NOUN
biotropia-204	36	15	was	be	AUX
biotropia-204	36	16	filtered	filter	VERB
biotropia-204	36	17	using	use	VERB
biotropia-204	36	18	microfilter	microfilter	NOUN
biotropia-204	36	19	,	,	PUNCT
biotropia-204	36	20	and	and	CCONJ
biotropia-204	36	21	then	then	ADV
biotropia-204	36	22	concentrated	concentrate	VERB
biotropia-204	36	23	further	far	ADV
biotropia-204	36	24	using	use	VERB
biotropia-204	36	25	10	10	NUM
biotropia-204	36	26	kd	kd	PROPN
biotropia-204	36	27	membrane	membrane	NOUN
biotropia-204	36	28	ultrafilter	ultrafilter	NOUN
biotropia-204	36	29	module	module	NOUN
biotropia-204	36	30	(	(	PUNCT
biotropia-204	36	31	minitan	minitan	ADJ
biotropia-204	36	32	,	,	PUNCT
biotropia-204	36	33	milipore	milipore	PROPN
biotropia-204	36	34	)	)	PUNCT
biotropia-204	36	35	.	.	PUNCT
biotropia-204	37	1	30	30	NUM
biotropia-204	37	2	isolation	isolation	NOUN
biotropia-204	37	3	and	and	CCONJ
biotropia-204	37	4	selection	selection	NOUN
biotropia-204	37	5	of	of	ADP
biotropia-204	37	6	alkaline	alkaline	ADJ
biotropia-204	37	7	proteolytic	proteolytic	ADJ
biotropia-204	37	8	bacteria	bacteria	NOUN
biotropia-204	37	9	budiasih	budiasih	VERB
biotropia-204	37	10	wahyuntari	wahyuntari	PROPN
biotropia-204	37	11	et	et	PROPN
biotropia-204	37	12	al	al	PROPN
biotropia-204	37	13	.	.	PROPN
biotropia-204	37	14	effect	effect	NOUN
biotropia-204	37	15	of	of	ADP
biotropia-204	37	16	ph	ph	ADJ
biotropia-204	37	17	and	and	CCONJ
biotropia-204	37	18	temperature	temperature	NOUN
biotropia-204	37	19	studies	study	NOUN
biotropia-204	37	20	the	the	DET
biotropia-204	37	21	effect	effect	NOUN
biotropia-204	37	22	of	of	ADP
biotropia-204	37	23	ph	ph	NOUN
biotropia-204	37	24	on	on	ADP
biotropia-204	37	25	protease	protease	NOUN
biotropia-204	37	26	activity	activity	NOUN
biotropia-204	37	27	produced	produce	VERB
biotropia-204	37	28	by	by	ADP
biotropia-204	37	29	the	the	DET
biotropia-204	37	30	selected	select	VERB
biotropia-204	37	31	isolates	isolate	NOUN
biotropia-204	37	32	was	be	AUX
biotropia-204	37	33	determined	determine	VERB
biotropia-204	37	34	at	at	ADP
biotropia-204	37	35	37	37	NUM
biotropia-204	37	36	°	°	NOUN
biotropia-204	37	37	c	c	NOUN
biotropia-204	37	38	in	in	ADP
biotropia-204	37	39	50	50	NUM
biotropia-204	37	40	mm	mm	NUM
biotropia-204	37	41	tris	tris	PROPN
biotropia-204	37	42	-	-	PUNCT
biotropia-204	37	43	hcl	hcl	NOUN
biotropia-204	37	44	buffer	buffer	NOUN
biotropia-204	37	45	at	at	ADP
biotropia-204	37	46	ph	ph	ADJ
biotropia-204	37	47	7	7	NUM
biotropia-204	37	48	-	-	SYM
biotropia-204	37	49	8	8	NUM
biotropia-204	37	50	and	and	CCONJ
biotropia-204	37	51	in	in	ADP
biotropia-204	37	52	glycine	glycine	NOUN
biotropia-204	37	53	-	-	PUNCT
biotropia-204	37	54	naoh	naoh	NOUN
biotropia-204	37	55	at	at	ADP
biotropia-204	37	56	ph	ph	PROPN
biotropia-204	37	57	9	9	NUM
biotropia-204	37	58	-	-	SYM
biotropia-204	37	59	11	11	NUM
biotropia-204	37	60	.	.	PUNCT
biotropia-204	38	1	to	to	PART
biotropia-204	38	2	determine	determine	VERB
biotropia-204	38	3	the	the	DET
biotropia-204	38	4	effect	effect	NOUN
biotropia-204	38	5	of	of	ADP
biotropia-204	38	6	temperature	temperature	NOUN
biotropia-204	38	7	on	on	ADP
biotropia-204	38	8	protease	protease	NOUN
biotropia-204	38	9	activity	activity	NOUN
biotropia-204	38	10	,	,	PUNCT
biotropia-204	38	11	the	the	DET
biotropia-204	38	12	assay	assay	NOUN
biotropia-204	38	13	was	be	AUX
biotropia-204	38	14	performed	perform	VERB
biotropia-204	38	15	in	in	ADP
biotropia-204	38	16	50	50	NUM
biotropia-204	38	17	mm	mm	NUM
biotropia-204	38	18	tris	tris	PROPN
biotropia-204	38	19	-	-	PUNCT
biotropia-204	38	20	hcl	hcl	NOUN
biotropia-204	38	21	buffer	buffer	NOUN
biotropia-204	38	22	(	(	PUNCT
biotropia-204	38	23	ph	ph	NOUN
biotropia-204	38	24	8)	8)	NUM
biotropia-204	38	25	at	at	ADP
biotropia-204	38	26	temperature	temperature	NOUN
biotropia-204	38	27	range	range	NOUN
biotropia-204	38	28	of	of	ADP
biotropia-204	38	29	30	30	NUM
biotropia-204	38	30	-	-	SYM
biotropia-204	38	31	70	70	NUM
biotropia-204	38	32	°	°	NOUN
biotropia-204	38	33	c	c	NOUN
biotropia-204	38	34	.	.	PUNCT
biotropia-204	39	1	effect	effect	NOUN
biotropia-204	39	2	of	of	ADP
biotropia-204	39	3	protease	protease	NOUN
biotropia-204	39	4	inhibitors	inhibitor	NOUN
biotropia-204	39	5	and	and	CCONJ
biotropia-204	39	6	metal	metal	NOUN
biotropia-204	39	7	ions	ion	NOUN
biotropia-204	39	8	the	the	DET
biotropia-204	39	9	concentrated	concentrated	ADJ
biotropia-204	39	10	enzyme	enzyme	NOUN
biotropia-204	39	11	solution	solution	NOUN
biotropia-204	39	12	was	be	AUX
biotropia-204	39	13	incubated	incubate	VERB
biotropia-204	39	14	in	in	ADP
biotropia-204	39	15	2	2	NUM
biotropia-204	39	16	and	and	CCONJ
biotropia-204	39	17	5	5	NUM
biotropia-204	39	18	mm	mm	NOUN
biotropia-204	39	19	edta	edta	NOUN
biotropia-204	39	20	or	or	CCONJ
biotropia-204	39	21	pmsf	pmsf	NOUN
biotropia-204	39	22	in	in	ADP
biotropia-204	39	23	50	50	NUM
biotropia-204	39	24	mm	mm	NOUN
biotropia-204	39	25	tris	tris	PROPN
biotropia-204	39	26	-	-	PUNCT
biotropia-204	39	27	hcl	hcl	PROPN
biotropia-204	39	28	at	at	ADP
biotropia-204	39	29	ph	ph	PROPN
biotropia-204	39	30	8	8	NUM
biotropia-204	39	31	for	for	ADP
biotropia-204	39	32	30	30	NUM
biotropia-204	39	33	minutes	minute	NOUN
biotropia-204	39	34	at	at	ADP
biotropia-204	39	35	5	5	NUM
biotropia-204	39	36	°	°	NOUN
biotropia-204	39	37	c	c	NOUN
biotropia-204	39	38	.	.	PUNCT
biotropia-204	40	1	the	the	DET
biotropia-204	40	2	same	same	ADJ
biotropia-204	40	3	concentration	concentration	NOUN
biotropia-204	40	4	of	of	ADP
biotropia-204	40	5	2	2	NUM
biotropia-204	40	6	and	and	CCONJ
biotropia-204	40	7	5	5	NUM
biotropia-204	40	8	mm	mm	NOUN
biotropia-204	40	9	mgcl2	mgcl2	PROPN
biotropia-204	40	10	,	,	PUNCT
biotropia-204	40	11	cacl2	cacl2	NOUN
biotropia-204	40	12	,	,	PUNCT
biotropia-204	40	13	zncl2	zncl2	NOUN
biotropia-204	40	14	,	,	PUNCT
biotropia-204	40	15	mncl2	mncl2	PROPN
biotropia-204	40	16	,	,	PUNCT
biotropia-204	40	17	fecl3	fecl3	PROPN
biotropia-204	40	18	and	and	CCONJ
biotropia-204	40	19	feso4	feso4	NOUN
biotropia-204	40	20	were	be	AUX
biotropia-204	40	21	also	also	ADV
biotropia-204	40	22	incubated	incubate	VERB
biotropia-204	40	23	with	with	ADP
biotropia-204	40	24	the	the	DET
biotropia-204	40	25	enzyme	enzyme	NOUN
biotropia-204	40	26	at	at	ADP
biotropia-204	40	27	5	5	NUM
biotropia-204	40	28	°	°	ADP
biotropia-204	40	29	c	c	NOUN
biotropia-204	40	30	for	for	ADP
biotropia-204	40	31	30	30	NUM
biotropia-204	40	32	minutes	minute	NOUN
biotropia-204	40	33	.	.	PUNCT
biotropia-204	41	1	residual	residual	ADJ
biotropia-204	41	2	activity	activity	NOUN
biotropia-204	41	3	was	be	AUX
biotropia-204	41	4	measured	measure	VERB
biotropia-204	41	5	under	under	ADP
biotropia-204	41	6	optimum	optimum	ADJ
biotropia-204	41	7	ph	ph	ADJ
biotropia-204	41	8	and	and	CCONJ
biotropia-204	41	9	temperature	temperature	NOUN
biotropia-204	41	10	.	.	PUNCT
biotropia-204	42	1	protease	protease	NOUN
biotropia-204	42	2	activity	activity	NOUN
biotropia-204	42	3	assayed	assay	VERB
biotropia-204	42	4	in	in	ADP
biotropia-204	42	5	the	the	DET
biotropia-204	42	6	absence	absence	NOUN
biotropia-204	42	7	of	of	ADP
biotropia-204	42	8	inhibitors	inhibitor	NOUN
biotropia-204	42	9	and	and	CCONJ
biotropia-204	42	10	metal	metal	NOUN
biotropia-204	42	11	ions	ion	NOUN
biotropia-204	42	12	was	be	AUX
biotropia-204	42	13	considered	consider	VERB
biotropia-204	42	14	as	as	ADP
biotropia-204	42	15	100	100	NUM
biotropia-204	42	16	%	%	NOUN
biotropia-204	42	17	.	.	PUNCT
biotropia-204	43	1	polyacrylamide	polyacrylamide	NOUN
biotropia-204	43	2	gel	gel	PROPN
biotropia-204	43	3	electrophoresis	electrophoresis	NOUN
biotropia-204	43	4	and	and	CCONJ
biotropia-204	43	5	zymogram	zymogram	NOUN
biotropia-204	43	6	analysis	analysis	NOUN
biotropia-204	43	7	zymogram	zymogram	NOUN
biotropia-204	43	8	method	method	NOUN
biotropia-204	43	9	was	be	AUX
biotropia-204	43	10	employed	employ	VERB
biotropia-204	43	11	to	to	PART
biotropia-204	43	12	determine	determine	VERB
biotropia-204	43	13	the	the	DET
biotropia-204	43	14	molecular	molecular	ADJ
biotropia-204	43	15	weight	weight	NOUN
biotropia-204	43	16	of	of	ADP
biotropia-204	43	17	the	the	DET
biotropia-204	43	18	protease	protease	NOUN
biotropia-204	43	19	.	.	PUNCT
biotropia-204	44	1	the	the	DET
biotropia-204	44	2	concentrated	concentrate	VERB
biotropia-204	44	3	enzyme	enzyme	NOUN
biotropia-204	44	4	solution	solution	NOUN
biotropia-204	44	5	was	be	AUX
biotropia-204	44	6	loaded	load	VERB
biotropia-204	44	7	into	into	ADP
biotropia-204	44	8	page	page	NOUN
biotropia-204	44	9	containing	contain	VERB
biotropia-204	44	10	skimmed	skim	VERB
biotropia-204	44	11	milk	milk	NOUN
biotropia-204	44	12	(	(	PUNCT
biotropia-204	44	13	0.2	0.2	NUM
biotropia-204	44	14	%	%	NOUN
biotropia-204	44	15	)	)	PUNCT
biotropia-204	44	16	which	which	PRON
biotropia-204	44	17	copolymerized	copolymerize	VERB
biotropia-204	44	18	with	with	ADP
biotropia-204	44	19	7.5	7.5	NUM
biotropia-204	44	20	%	%	NOUN
biotropia-204	44	21	acrylamide	acrylamide	NOUN
biotropia-204	44	22	.	.	PUNCT
biotropia-204	45	1	electrophoresis	electrophoresis	NOUN
biotropia-204	45	2	was	be	AUX
biotropia-204	45	3	run	run	VERB
biotropia-204	45	4	at	at	ADP
biotropia-204	45	5	110	110	NUM
biotropia-204	45	6	v	v	NOUN
biotropia-204	45	7	,	,	PUNCT
biotropia-204	45	8	90	90	NUM
biotropia-204	45	9	ma	ma	NOUN
biotropia-204	45	10	for	for	ADP
biotropia-204	45	11	1.5	1.5	NUM
biotropia-204	45	12	hours	hour	NOUN
biotropia-204	45	13	.	.	PUNCT
biotropia-204	46	1	after	after	ADP
biotropia-204	46	2	completing	complete	VERB
biotropia-204	46	3	electrophoresis	electrophoresis	NOUN
biotropia-204	46	4	,	,	PUNCT
biotropia-204	46	5	the	the	DET
biotropia-204	46	6	gel	gel	NOUN
biotropia-204	46	7	was	be	AUX
biotropia-204	46	8	soaked	soak	VERB
biotropia-204	46	9	in	in	ADP
biotropia-204	46	10	50	50	NUM
biotropia-204	46	11	mm	mm	NOUN
biotropia-204	46	12	tris	tris	PROPN
biotropia-204	46	13	-	-	PUNCT
biotropia-204	46	14	hcl	hcl	NOUN
biotropia-204	46	15	containing	contain	VERB
biotropia-204	46	16	5	5	NUM
biotropia-204	46	17	mm	mm	NOUN
biotropia-204	46	18	cacl2	cacl2	NOUN
biotropia-204	46	19	then	then	ADV
biotropia-204	46	20	incubated	incubate	VERB
biotropia-204	46	21	at	at	ADP
biotropia-204	46	22	optimum	optimum	ADJ
biotropia-204	46	23	ph	ph	NOUN
biotropia-204	46	24	and	and	CCONJ
biotropia-204	46	25	temperature	temperature	NOUN
biotropia-204	46	26	of	of	ADP
biotropia-204	46	27	the	the	DET
biotropia-204	46	28	enzyme	enzyme	NOUN
biotropia-204	46	29	.	.	PUNCT
biotropia-204	47	1	visualization	visualization	NOUN
biotropia-204	47	2	of	of	ADP
biotropia-204	47	3	the	the	DET
biotropia-204	47	4	enzyme	enzyme	NOUN
biotropia-204	47	5	staining	stain	VERB
biotropia-204	47	6	activity	activity	NOUN
biotropia-204	47	7	was	be	AUX
biotropia-204	47	8	carried	carry	VERB
biotropia-204	47	9	out	out	ADP
biotropia-204	47	10	by	by	ADP
biotropia-204	47	11	staining	stain	VERB
biotropia-204	47	12	the	the	DET
biotropia-204	47	13	gel	gel	NOUN
biotropia-204	47	14	in	in	ADP
biotropia-204	47	15	commassie	commassie	DET
biotropia-204	47	16	brilliant	brilliant	ADJ
biotropia-204	47	17	blue	blue	ADJ
biotropia-204	47	18	solution	solution	NOUN
biotropia-204	47	19	.	.	PUNCT
biotropia-204	48	1	clear	clear	ADJ
biotropia-204	48	2	band	band	NOUN
biotropia-204	48	3	on	on	ADP
biotropia-204	48	4	the	the	DET
biotropia-204	48	5	skim	skim	NOUN
biotropia-204	48	6	milk	milk	NOUN
biotropia-204	48	7	containing	contain	VERB
biotropia-204	48	8	electrophoresis	electrophoresis	NOUN
biotropia-204	48	9	gel	gel	NOUN
biotropia-204	48	10	indicated	indicate	VERB
biotropia-204	48	11	the	the	DET
biotropia-204	48	12	protein	protein	NOUN
biotropia-204	48	13	band	band	NOUN
biotropia-204	48	14	which	which	PRON
biotropia-204	48	15	had	have	AUX
biotropia-204	48	16	shown	show	VERB
biotropia-204	48	17	proteolytic	proteolytic	ADJ
biotropia-204	48	18	activity	activity	NOUN
biotropia-204	48	19	.	.	PUNCT
biotropia-204	49	1	the	the	DET
biotropia-204	49	2	standard	standard	ADJ
biotropia-204	49	3	molecular	molecular	ADJ
biotropia-204	49	4	weight	weight	NOUN
biotropia-204	49	5	marker	marker	NOUN
biotropia-204	49	6	used	use	VERB
biotropia-204	49	7	was	be	AUX
biotropia-204	49	8	hmw	hmw	PROPN
biotropia-204	49	9	protein	protein	NOUN
biotropia-204	49	10	standard	standard	ADJ
biotropia-204	49	11	marker	marker	NOUN
biotropia-204	49	12	(	(	PUNCT
biotropia-204	49	13	amersham	amersham	PROPN
biotropia-204	49	14	)	)	PUNCT
biotropia-204	49	15	.	.	PUNCT
biotropia-204	50	1	results	result	NOUN
biotropia-204	50	2	and	and	CCONJ
biotropia-204	50	3	discussion	discussion	NOUN
biotropia-204	50	4	isolation	isolation	NOUN
biotropia-204	50	5	of	of	ADP
biotropia-204	50	6	protease	protease	NOUN
biotropia-204	50	7	-	-	PUNCT
biotropia-204	50	8	producing	produce	VERB
biotropia-204	50	9	bacteria	bacteria	NOUN
biotropia-204	50	10	there	there	PRON
biotropia-204	50	11	were	be	VERB
biotropia-204	50	12	9	9	NUM
biotropia-204	50	13	isolates	isolate	NOUN
biotropia-204	50	14	which	which	PRON
biotropia-204	50	15	showed	show	VERB
biotropia-204	50	16	proteolytic	proteolytic	ADJ
biotropia-204	50	17	activity	activity	NOUN
biotropia-204	50	18	based	base	VERB
biotropia-204	50	19	on	on	ADP
biotropia-204	50	20	clear	clear	ADJ
biotropia-204	50	21	zone	zone	NOUN
biotropia-204	50	22	formation	formation	NOUN
biotropia-204	50	23	on	on	ADP
biotropia-204	50	24	skim	skim	NOUN
biotropia-204	50	25	milk	milk	NOUN
biotropia-204	50	26	containing	contain	VERB
biotropia-204	50	27	media	medium	NOUN
biotropia-204	50	28	.	.	PUNCT
biotropia-204	51	1	four	four	NUM
biotropia-204	51	2	isolates	isolate	NOUN
biotropia-204	51	3	grew	grow	VERB
biotropia-204	51	4	well	well	ADV
biotropia-204	51	5	on	on	ADP
biotropia-204	51	6	media	medium	NOUN
biotropia-204	51	7	at	at	ADP
biotropia-204	51	8	ph	ph	PROPN
biotropia-204	51	9	9	9	NUM
biotropia-204	51	10	m	m	NOUN
biotropia-204	51	11	,	,	PUNCT
biotropia-204	51	12	another	another	DET
biotropia-204	51	13	four	four	NUM
biotropia-204	51	14	isolates	isolate	NOUN
biotropia-204	51	15	at	at	ADP
biotropia-204	51	16	ph	ph	PROPN
biotropia-204	51	17	10	10	NUM
biotropia-204	51	18	,	,	PUNCT
biotropia-204	51	19	and	and	CCONJ
biotropia-204	51	20	only	only	ADV
biotropia-204	51	21	one	one	NUM
biotropia-204	51	22	isolate	isolate	NOUN
biotropia-204	51	23	at	at	ADP
biotropia-204	51	24	ph	ph	PROPN
biotropia-204	51	25	11	11	NUM
biotropia-204	51	26	.	.	PUNCT
biotropia-204	51	27	table	table	NOUN
biotropia-204	51	28	1	1	NUM
biotropia-204	51	29	shows	show	VERB
biotropia-204	51	30	the	the	DET
biotropia-204	51	31	physical	physical	ADJ
biotropia-204	51	32	properties	property	NOUN
biotropia-204	51	33	,	,	PUNCT
biotropia-204	51	34	proteolytic	proteolytic	ADJ
biotropia-204	51	35	index	index	NOUN
biotropia-204	51	36	,	,	PUNCT
biotropia-204	51	37	and	and	CCONJ
biotropia-204	51	38	protease	protease	NOUN
biotropia-204	51	39	activity	activity	NOUN
biotropia-204	51	40	of	of	ADP
biotropia-204	51	41	the	the	DET
biotropia-204	51	42	isolates	isolate	NOUN
biotropia-204	51	43	.	.	PUNCT
biotropia-204	52	1	proteolytic	proteolytic	ADJ
biotropia-204	52	2	activity	activity	NOUN
biotropia-204	52	3	of	of	ADP
biotropia-204	52	4	isolate	isolate	PROPN
biotropia-204	52	5	t4	t4	PROPN
biotropia-204	52	6	decreased	decrease	VERB
biotropia-204	52	7	in	in	ADP
biotropia-204	52	8	the	the	DET
biotropia-204	52	9	following	follow	VERB
biotropia-204	52	10	experiment	experiment	NOUN
biotropia-204	52	11	,	,	PUNCT
biotropia-204	52	12	and	and	CCONJ
biotropia-204	52	13	therefore	therefore	ADV
biotropia-204	52	14	the	the	DET
biotropia-204	52	15	isolate	isolate	NOUN
biotropia-204	52	16	was	be	AUX
biotropia-204	52	17	excluded	exclude	VERB
biotropia-204	52	18	.	.	PUNCT
biotropia-204	53	1	isolate	isolate	PROPN
biotropia-204	53	2	d2	d2	PROPN
biotropia-204	53	3	,	,	PUNCT
biotropia-204	53	4	d7	d7	PROPN
biotropia-204	53	5	and	and	CCONJ
biotropia-204	53	6	dl	dl	PROPN
biotropia-204	53	7	l	l	PROPN
biotropia-204	53	8	were	be	AUX
biotropia-204	53	9	selected	select	VERB
biotropia-204	53	10	based	base	VERB
biotropia-204	53	11	on	on	ADP
biotropia-204	53	12	their	their	PRON
biotropia-204	53	13	proteolytic	proteolytic	ADJ
biotropia-204	53	14	index	index	NOUN
biotropia-204	53	15	and	and	CCONJ
biotropia-204	53	16	ability	ability	NOUN
biotropia-204	53	17	to	to	PART
biotropia-204	53	18	produce	produce	VERB
biotropia-204	53	19	protease	protease	NOUN
biotropia-204	53	20	in	in	ADP
biotropia-204	53	21	submerged	submerged	ADJ
biotropia-204	53	22	culture	culture	NOUN
biotropia-204	53	23	.	.	PUNCT
biotropia-204	54	1	biochemical	biochemical	ADJ
biotropia-204	54	2	characteristics	characteristic	NOUN
biotropia-204	54	3	of	of	ADP
biotropia-204	54	4	the	the	DET
biotropia-204	54	5	selected	select	VERB
biotropia-204	54	6	isolates	isolate	NOUN
biotropia-204	54	7	are	be	AUX
biotropia-204	54	8	displayed	display	VERB
biotropia-204	54	9	in	in	ADP
biotropia-204	54	10	table	table	NOUN
biotropia-204	54	11	2	2	NUM
biotropia-204	54	12	.	.	PUNCT
biotropia-204	54	13	based	base	VERB
biotropia-204	54	14	on	on	ADP
biotropia-204	54	15	their	their	PRON
biotropia-204	54	16	physical	physical	ADJ
biotropia-204	54	17	,	,	PUNCT
biotropia-204	54	18	and	and	CCONJ
biotropia-204	54	19	biochemical	biochemical	ADJ
biotropia-204	54	20	characteristics	characteristic	NOUN
biotropia-204	54	21	,	,	PUNCT
biotropia-204	54	22	it	it	PRON
biotropia-204	54	23	can	can	AUX
biotropia-204	54	24	be	be	AUX
biotropia-204	54	25	concluded	conclude	VERB
biotropia-204	54	26	that	that	SCONJ
biotropia-204	54	27	the	the	DET
biotropia-204	54	28	isolates	isolate	NOUN
biotropia-204	54	29	(	(	PUNCT
biotropia-204	54	30	d2	d2	PROPN
biotropia-204	54	31	,	,	PUNCT
biotropia-204	54	32	d7	d7	PROPN
biotropia-204	54	33	and	and	CCONJ
biotropia-204	54	34	dll	dll	NOUN
biotropia-204	54	35	)	)	PUNCT
biotropia-204	54	36	most	most	ADV
biotropia-204	54	37	likely	likely	ADV
biotropia-204	54	38	belong	belong	VERB
biotropia-204	54	39	to	to	ADP
biotropia-204	54	40	bacillus	bacillus	NOUN
biotropia-204	54	41	spp	spp	NOUN
biotropia-204	54	42	.	.	PROPN
biotropia-204	55	1	31	31	NUM
biotropia-204	55	2	selection	selection	NOUN
biotropia-204	55	3	of	of	ADP
biotropia-204	55	4	bacterial	bacterial	ADJ
biotropia-204	55	5	strain	strain	NOUN
biotropia-204	55	6	.	.	PUNCT
biotropia-204	56	1	further	further	ADJ
biotropia-204	56	2	selection	selection	NOUN
biotropia-204	56	3	of	of	ADP
biotropia-204	56	4	the	the	DET
biotropia-204	56	5	best	good	ADJ
biotropia-204	56	6	proteolytic	proteolytic	ADJ
biotropia-204	56	7	bacteria	bacteria	NOUN
biotropia-204	56	8	was	be	AUX
biotropia-204	56	9	based	base	VERB
biotropia-204	56	10	on	on	ADP
biotropia-204	56	11	the	the	DET
biotropia-204	56	12	productivity	productivity	NOUN
biotropia-204	56	13	of	of	ADP
biotropia-204	56	14	the	the	DET
biotropia-204	56	15	isolate	isolate	NOUN
biotropia-204	56	16	at	at	AUX
biotropia-204	56	17	die	die	VERB
biotropia-204	56	18	highest	high	ADJ
biotropia-204	56	19	ph	ph	VERB
biotropia-204	56	20	.	.	PUNCT
biotropia-204	57	1	all	all	DET
biotropia-204	57	2	the	the	DET
biotropia-204	57	3	isolates	isolate	NOUN
biotropia-204	57	4	screened	screen	VERB
biotropia-204	57	5	were	be	AUX
biotropia-204	57	6	grown	grow	VERB
biotropia-204	57	7	at	at	ADP
biotropia-204	57	8	three	three	NUM
biotropia-204	57	9	different	different	ADJ
biotropia-204	57	10	levels	level	NOUN
biotropia-204	57	11	of	of	ADP
biotropia-204	57	12	ph	ph	NOUN
biotropia-204	57	13	(	(	PUNCT
biotropia-204	57	14	9	9	NUM
biotropia-204	57	15	;	;	PUNCT
biotropia-204	57	16	10	10	NUM
biotropia-204	57	17	and	and	CCONJ
biotropia-204	57	18	11	11	NUM
biotropia-204	57	19	)	)	PUNCT
biotropia-204	57	20	.	.	PUNCT
biotropia-204	58	1	however	however	ADV
biotropia-204	58	2	,	,	PUNCT
biotropia-204	58	3	all	all	DET
biotropia-204	58	4	isolates	isolate	NOUN
biotropia-204	58	5	did	do	AUX
biotropia-204	58	6	not	not	PART
biotropia-204	58	7	grow	grow	VERB
biotropia-204	58	8	well	well	ADV
biotropia-204	58	9	at	at	ADP
biotropia-204	58	10	ph	ph	ADJ
biotropia-204	58	11	32	32	NUM
biotropia-204	58	12	isolation	isolation	NOUN
biotropia-204	58	13	and	and	CCONJ
biotropia-204	58	14	selection	selection	NOUN
biotropia-204	58	15	of	of	ADP
biotropia-204	58	16	alkaline	alkaline	ADJ
biotropia-204	58	17	proteolytic	proteolytic	ADJ
biotropia-204	58	18	bacteria	bacteria	NOUN
biotropia-204	58	19	budiasih	budiasih	VERB
biotropia-204	58	20	wahyuntari	wahyuntari	PROPN
biotropia-204	58	21	el	el	PROPN
biotropia-204	58	22	al	al	PROPN
biotropia-204	58	23	.	.	PROPN
biotropia-204	59	1	11	11	NUM
biotropia-204	59	2	.	.	PUNCT
biotropia-204	60	1	figures	figure	NOUN
biotropia-204	60	2	1	1	NUM
biotropia-204	60	3	,	,	PUNCT
biotropia-204	60	4	2	2	NUM
biotropia-204	60	5	and	and	CCONJ
biotropia-204	60	6	3	3	NUM
biotropia-204	60	7	reveal	reveal	VERB
biotropia-204	60	8	growth	growth	NOUN
biotropia-204	60	9	and	and	CCONJ
biotropia-204	60	10	protease	protease	NOUN
biotropia-204	60	11	production	production	NOUN
biotropia-204	60	12	rates	rate	NOUN
biotropia-204	60	13	of	of	ADP
biotropia-204	60	14	d2	d2	PROPN
biotropia-204	60	15	,	,	PUNCT
biotropia-204	60	16	d7	d7	PROPN
biotropia-204	60	17	,	,	PUNCT
biotropia-204	60	18	and	and	CCONJ
biotropia-204	60	19	dl	dl	PROPN
biotropia-204	60	20	l	l	NOUN
biotropia-204	60	21	,	,	PUNCT
biotropia-204	60	22	respectively	respectively	ADV
biotropia-204	60	23	.	.	PUNCT
biotropia-204	61	1	the	the	DET
biotropia-204	61	2	growth	growth	NOUN
biotropia-204	61	3	rate	rate	NOUN
biotropia-204	61	4	of	of	ADP
biotropia-204	61	5	isolate	isolate	PROPN
biotropia-204	61	6	d2	d2	PROPN
biotropia-204	61	7	was	be	AUX
biotropia-204	61	8	only	only	ADV
biotropia-204	61	9	slightly	slightly	ADV
biotropia-204	61	10	better	well	ADJ
biotropia-204	61	11	at	at	ADP
biotropia-204	61	12	ph	ph	ADJ
biotropia-204	61	13	9	9	NUM
biotropia-204	61	14	as	as	SCONJ
biotropia-204	61	15	compared	compare	VERB
biotropia-204	61	16	to	to	ADP
biotropia-204	61	17	ph	ph	PROPN
biotropia-204	61	18	10	10	NUM
biotropia-204	61	19	.	.	PUNCT
biotropia-204	62	1	however	however	ADV
biotropia-204	62	2	,	,	PUNCT
biotropia-204	62	3	its	its	PRON
biotropia-204	62	4	protease	protease	NOUN
biotropia-204	62	5	production	production	NOUN
biotropia-204	62	6	rate	rate	NOUN
biotropia-204	62	7	at	at	ADP
biotropia-204	62	8	ph	ph	PROPN
biotropia-204	62	9	9	9	NUM
biotropia-204	62	10	was	be	AUX
biotropia-204	62	11	higher	high	ADJ
biotropia-204	62	12	than	than	ADP
biotropia-204	62	13	that	that	PRON
biotropia-204	62	14	at	at	ADP
biotropia-204	62	15	ph	ph	PROPN
biotropia-204	62	16	10	10	NUM
biotropia-204	62	17	.	.	PUNCT
biotropia-204	63	1	the	the	DET
biotropia-204	63	2	highest	high	ADJ
biotropia-204	63	3	protease	protease	NOUN
biotropia-204	63	4	production	production	NOUN
biotropia-204	63	5	of	of	ADP
biotropia-204	63	6	isolate	isolate	PROPN
biotropia-204	63	7	d2	d2	PROPN
biotropia-204	63	8	was	be	AUX
biotropia-204	63	9	at	at	ADP
biotropia-204	63	10	36	36	NUM
biotropia-204	63	11	-	-	PUNCT
biotropia-204	63	12	hour	hour	NOUN
biotropia-204	63	13	fermentation	fermentation	NOUN
biotropia-204	63	14	with	with	ADP
biotropia-204	63	15	a	a	DET
biotropia-204	63	16	protease	protease	NOUN
biotropia-204	63	17	concentration	concentration	NOUN
biotropia-204	63	18	of	of	ADP
biotropia-204	63	19	3.65	3.65	NUM
biotropia-204	63	20	u	u	NOUN
biotropia-204	63	21	/	/	SYM
biotropia-204	63	22	l.	l.	PROPN
biotropia-204	63	23	the	the	DET
biotropia-204	63	24	growth	growth	NOUN
biotropia-204	63	25	rate	rate	NOUN
biotropia-204	63	26	of	of	ADP
biotropia-204	63	27	isolate	isolate	NOUN
biotropia-204	63	28	d7	d7	PROPN
biotropia-204	63	29	at	at	ADP
biotropia-204	63	30	ph	ph	PROPN
biotropia-204	63	31	9	9	NUM
biotropia-204	63	32	was	be	AUX
biotropia-204	63	33	slightly	slightly	ADV
biotropia-204	63	34	better	well	ADJ
biotropia-204	63	35	than	than	ADP
biotropia-204	63	36	at	at	ADP
biotropia-204	63	37	ph	ph	PROPN
biotropia-204	63	38	10	10	NUM
biotropia-204	63	39	.	.	PUNCT
biotropia-204	64	1	however	however	ADV
biotropia-204	64	2	,	,	PUNCT
biotropia-204	64	3	the	the	DET
biotropia-204	64	4	protease	protease	NOUN
biotropia-204	64	5	production	production	NOUN
biotropia-204	64	6	rate	rate	NOUN
biotropia-204	64	7	was	be	AUX
biotropia-204	64	8	higher	high	ADJ
biotropia-204	64	9	at	at	ADP
biotropia-204	64	10	ph	ph	PROPN
biotropia-204	64	11	10	10	NUM
biotropia-204	64	12	after	after	ADP
biotropia-204	64	13	36	36	NUM
biotropia-204	64	14	hours	hour	NOUN
biotropia-204	64	15	incubation	incubation	NOUN
biotropia-204	64	16	with	with	ADP
biotropia-204	64	17	the	the	DET
biotropia-204	64	18	activity	activity	NOUN
biotropia-204	64	19	of	of	ADP
biotropia-204	64	20	10.6	10.6	NUM
biotropia-204	64	21	u	u	NOUN
biotropia-204	64	22	/	/	SYM
biotropia-204	64	23	l.	l.	PROPN
biotropia-204	64	24	figure	figure	NOUN
biotropia-204	64	25	3	3	NUM
biotropia-204	64	26	shows	show	VERB
biotropia-204	64	27	that	that	SCONJ
biotropia-204	64	28	the	the	DET
biotropia-204	64	29	growth	growth	NOUN
biotropia-204	64	30	rate	rate	NOUN
biotropia-204	64	31	and	and	CCONJ
biotropia-204	64	32	protease	protease	NOUN
biotropia-204	64	33	production	production	NOUN
biotropia-204	64	34	of	of	ADP
biotropia-204	64	35	isolate	isolate	NOUN
biotropia-204	64	36	dl	dl	PROPN
biotropia-204	64	37	1	1	NUM
biotropia-204	64	38	was	be	AUX
biotropia-204	64	39	better	well	ADJ
biotropia-204	64	40	at	at	ADP
biotropia-204	64	41	ph	ph	NOUN
biotropia-204	64	42	9	9	NUM
biotropia-204	64	43	and	and	CCONJ
biotropia-204	64	44	with	with	ADP
biotropia-204	64	45	the	the	DET
biotropia-204	64	46	highest	high	ADJ
biotropia-204	64	47	protease	protease	NOUN
biotropia-204	64	48	production	production	NOUN
biotropia-204	64	49	at	at	ADP
biotropia-204	64	50	24	24	NUM
biotropia-204	64	51	hour	hour	NOUN
biotropia-204	64	52	incubation	incubation	NOUN
biotropia-204	64	53	time	time	NOUN
biotropia-204	64	54	with	with	ADP
biotropia-204	64	55	enzyme	enzyme	NOUN
biotropia-204	64	56	activity	activity	NOUN
biotropia-204	64	57	of	of	ADP
biotropia-204	64	58	5.8	5.8	NUM
biotropia-204	64	59	u	u	NOUN
biotropia-204	64	60	/	/	SYM
biotropia-204	64	61	l.	l.	PROPN
biotropia-204	65	1	the	the	DET
biotropia-204	65	2	protease	protease	NOUN
biotropia-204	65	3	produced	produce	VERB
biotropia-204	65	4	by	by	ADP
biotropia-204	65	5	all	all	DET
biotropia-204	65	6	isolates	isolate	NOUN
biotropia-204	65	7	in	in	ADP
biotropia-204	65	8	skim	skim	NOUN
biotropia-204	65	9	milk	milk	NOUN
biotropia-204	65	10	containing	contain	VERB
biotropia-204	65	11	medium	medium	NOUN
biotropia-204	65	12	was	be	AUX
biotropia-204	65	13	much	much	ADV
biotropia-204	65	14	higher	high	ADJ
biotropia-204	65	15	as	as	ADP
biotropia-204	65	16	compared	compare	VERB
biotropia-204	65	17	to	to	ADP
biotropia-204	65	18	enzyme	enzyme	NOUN
biotropia-204	65	19	produced	produce	VERB
biotropia-204	65	20	in	in	ADP
biotropia-204	65	21	luria	luria	ADJ
biotropia-204	65	22	broth	broth	NOUN
biotropia-204	65	23	used	use	VERB
biotropia-204	65	24	in	in	ADP
biotropia-204	65	25	early	early	ADJ
biotropia-204	65	26	selection	selection	NOUN
biotropia-204	65	27	of	of	ADP
biotropia-204	65	28	isolates	isolate	NOUN
biotropia-204	65	29	.	.	PUNCT
biotropia-204	66	1	the	the	DET
biotropia-204	66	2	data	datum	NOUN
biotropia-204	66	3	indicated	indicate	VERB
biotropia-204	66	4	that	that	SCONJ
biotropia-204	66	5	the	the	DET
biotropia-204	66	6	enzyme	enzyme	NOUN
biotropia-204	66	7	produced	produce	VERB
biotropia-204	66	8	by	by	ADP
biotropia-204	66	9	all	all	DET
biotropia-204	66	10	isolates	isolate	NOUN
biotropia-204	66	11	observed	observe	VERB
biotropia-204	66	12	were	be	AUX
biotropia-204	66	13	inducible	inducible	ADJ
biotropia-204	66	14	ones	one	NOUN
biotropia-204	66	15	.	.	PUNCT
biotropia-204	67	1	among	among	ADP
biotropia-204	67	2	the	the	DET
biotropia-204	67	3	three	three	NUM
biotropia-204	67	4	isolates	isolate	NOUN
biotropia-204	67	5	observed	observe	VERB
biotropia-204	67	6	,	,	PUNCT
biotropia-204	67	7	isolate	isolate	PROPN
biotropia-204	67	8	d2	d2	PROPN
biotropia-204	67	9	produced	produce	VERB
biotropia-204	67	10	the	the	DET
biotropia-204	67	11	highest	high	ADJ
biotropia-204	67	12	protease	protease	NOUN
biotropia-204	67	13	,	,	PUNCT
biotropia-204	67	14	which	which	PRON
biotropia-204	67	15	showed	show	VERB
biotropia-204	67	16	that	that	SCONJ
biotropia-204	67	17	the	the	DET
biotropia-204	67	18	isolate	isolate	NOUN
biotropia-204	67	19	was	be	AUX
biotropia-204	67	20	the	the	DET
biotropia-204	67	21	most	most	ADV
biotropia-204	67	22	tolerable	tolerable	ADJ
biotropia-204	67	23	to	to	ADP
biotropia-204	67	24	alkaline	alkaline	NOUN
biotropia-204	67	25	condition	condition	NOUN
biotropia-204	67	26	.	.	PUNCT
biotropia-204	68	1	it	it	PRON
biotropia-204	68	2	was	be	AUX
biotropia-204	68	3	assumed	assume	VERB
biotropia-204	68	4	that	that	SCONJ
biotropia-204	68	5	the	the	DET
biotropia-204	68	6	enzyme	enzyme	NOUN
biotropia-204	68	7	belonged	belong	VERB
biotropia-204	68	8	to	to	ADP
biotropia-204	68	9	alkaline	alkaline	ADJ
biotropia-204	68	10	protease	protease	NOUN
biotropia-204	68	11	family	family	NOUN
biotropia-204	68	12	.	.	PUNCT
biotropia-204	69	1	therefore	therefore	ADV
biotropia-204	69	2	,	,	PUNCT
biotropia-204	69	3	t	t	PROPN
biotropia-204	69	4	he	he	PRON
biotropia-204	69	5	enzyme	enzyme	NOUN
biotropia-204	69	6	of	of	ADP
biotropia-204	69	7	the	the	DET
biotropia-204	69	8	isolate	isolate	NOUN
biotropia-204	69	9	was	be	AUX
biotropia-204	69	10	selected	select	VERB
biotropia-204	69	11	for	for	ADP
biotropia-204	69	12	further	further	ADJ
biotropia-204	69	13	study	study	NOUN
biotropia-204	69	14	.	.	PUNCT
biotropia-204	69	15	  	  	SPACE
biotropia-204	70	1	isolation	isolation	NOUN
biotropia-204	70	2	and	and	CCONJ
biotropia-204	70	3	selection	selection	NOUN
biotropia-204	70	4	of	of	ADP
biotropia-204	70	5	alkaline	alkaline	ADJ
biotropia-204	70	6	proteolytic	proteolytic	ADJ
biotropia-204	70	7	bacteria	bacteria	NOUN
biotropia-204	70	8	budiasih	budiasih	VERB
biotropia-204	70	9	wahyuntari	wahyuntari	PROPN
biotropia-204	70	10	et	et	PROPN
biotropia-204	70	11	al	al	PROPN
biotropia-204	70	12	.	.	PROPN
biotropia-204	70	13	effect	effect	NOUN
biotropia-204	70	14	of	of	ADP
biotropia-204	70	15	ph	ph	ADJ
biotropia-204	70	16	and	and	CCONJ
biotropia-204	70	17	temperature	temperature	NOUN
biotropia-204	70	18	study	study	NOUN
biotropia-204	70	19	on	on	ADP
biotropia-204	70	20	protease	protease	NOUN
biotropia-204	70	21	activity	activity	NOUN
biotropia-204	70	22	optimum	optimum	ADJ
biotropia-204	70	23	activity	activity	NOUN
biotropia-204	70	24	of	of	ADP
biotropia-204	70	25	d2	d2	PROPN
biotropia-204	70	26	isolate	isolate	NOUN
biotropia-204	70	27	protease	protease	NOUN
biotropia-204	70	28	was	be	AUX
biotropia-204	70	29	observed	observe	VERB
biotropia-204	70	30	at	at	ADP
biotropia-204	70	31	ph	ph	PROPN
biotropia-204	70	32	8	8	NUM
biotropia-204	70	33	and	and	CCONJ
biotropia-204	70	34	60uc	60uc	ADJ
biotropia-204	70	35	(	(	PUNCT
biotropia-204	70	36	figure	figure	NOUN
biotropia-204	70	37	4	4	NUM
biotropia-204	70	38	)	)	PUNCT
biotropia-204	70	39	.	.	PUNCT
biotropia-204	71	1	the	the	DET
biotropia-204	71	2	enzyme	enzyme	NOUN
biotropia-204	71	3	relative	relative	ADJ
biotropia-204	71	4	activity	activity	NOUN
biotropia-204	71	5	was	be	AUX
biotropia-204	71	6	70	70	NUM
biotropia-204	71	7	%	%	NOUN
biotropia-204	71	8	at	at	ADP
biotropia-204	71	9	ph	ph	PROPN
biotropia-204	71	10	7	7	NUM
biotropia-204	71	11	and	and	CCONJ
biotropia-204	71	12	9.5	9.5	NUM
biotropia-204	71	13	and	and	CCONJ
biotropia-204	71	14	at	at	ADP
biotropia-204	71	15	52	52	NUM
biotropia-204	71	16	and	and	CCONJ
biotropia-204	71	17	62	62	NUM
biotropia-204	71	18	°	°	NOUN
biotropia-204	71	19	c	c	NOUN
biotropia-204	71	20	.	.	PUNCT
biotropia-204	72	1	these	these	DET
biotropia-204	72	2	data	datum	NOUN
biotropia-204	72	3	show	show	VERB
biotropia-204	72	4	that	that	SCONJ
biotropia-204	72	5	the	the	DET
biotropia-204	72	6	enzyme	enzyme	NOUN
biotropia-204	72	7	is	be	AUX
biotropia-204	72	8	an	an	DET
biotropia-204	72	9	alkalo	alkalo	NOUN
biotropia-204	72	10	and	and	CCONJ
biotropia-204	72	11	thermotolerant	thermotolerant	NOUN
biotropia-204	72	12	one	one	NUM
biotropia-204	72	13	.	.	PUNCT
biotropia-204	73	1	studies	study	NOUN
biotropia-204	73	2	on	on	ADP
biotropia-204	73	3	isolation	isolation	NOUN
biotropia-204	73	4	and	and	CCONJ
biotropia-204	73	5	characterization	characterization	NOUN
biotropia-204	73	6	of	of	ADP
biotropia-204	73	7	alkaline	alkaline	NOUN
biotropia-204	73	8	protease	protease	NOUN
biotropia-204	73	9	have	have	AUX
biotropia-204	73	10	been	be	AUX
biotropia-204	73	11	ongoing	ongoing	ADJ
biotropia-204	73	12	for	for	ADP
biotropia-204	73	13	the	the	DET
biotropia-204	73	14	last	last	ADJ
biotropia-204	73	15	three	three	NUM
biotropia-204	73	16	decades	decade	NOUN
biotropia-204	73	17	(	(	PUNCT
biotropia-204	73	18	horikoshi	horikoshi	PROPN
biotropia-204	73	19	1996	1996	NUM
biotropia-204	73	20	and	and	CCONJ
biotropia-204	73	21	kumar	kumar	PROPN
biotropia-204	73	22	and	and	CCONJ
biotropia-204	73	23	takagi	takagi	PROPN
biotropia-204	73	24	,	,	PUNCT
biotropia-204	73	25	1998	1998	NUM
biotropia-204	73	26	)	)	PUNCT
biotropia-204	73	27	.	.	PUNCT
biotropia-204	74	1	these	these	PRON
biotropia-204	74	2	are	be	AUX
biotropia-204	74	3	the	the	DET
biotropia-204	74	4	most	most	ADV
biotropia-204	74	5	recent	recent	ADJ
biotropia-204	74	6	studies	study	NOUN
biotropia-204	74	7	on	on	ADP
biotropia-204	74	8	the	the	DET
biotropia-204	74	9	subject	subject	NOUN
biotropia-204	74	10	.	.	PUNCT
biotropia-204	75	1	singh	singh	PROPN
biotropia-204	75	2	et	et	PROPN
biotropia-204	75	3	al	al	PROPN
biotropia-204	75	4	.	.	PROPN
biotropia-204	76	1	(	(	PUNCT
biotropia-204	76	2	2001	2001	NUM
biotropia-204	76	3	)	)	PUNCT
biotropia-204	76	4	reported	report	VERB
biotropia-204	76	5	that	that	SCONJ
biotropia-204	76	6	optimum	optimum	ADJ
biotropia-204	76	7	activity	activity	NOUN
biotropia-204	76	8	of	of	ADP
biotropia-204	76	9	bacillus	bacillus	NOUN
biotropia-204	76	10	sp	sp	NOUN
biotropia-204	76	11	.	.	PUNCT
biotropia-204	76	12	ssr1	ssr1	PROPN
biotropia-204	76	13	was	be	AUX
biotropia-204	76	14	reached	reach	VERB
biotropia-204	76	15	at	at	ADP
biotropia-204	76	16	the	the	DET
biotropia-204	76	17	range	range	NOUN
biotropia-204	76	18	of	of	ADP
biotropia-204	76	19	ph	ph	ADJ
biotropia-204	76	20	8	8	NUM
biotropia-204	76	21	-	-	SYM
biotropia-204	76	22	11	11	NUM
biotropia-204	76	23	,	,	PUNCT
biotropia-204	76	24	40	40	NUM
biotropia-204	76	25	°	°	NOUN
biotropia-204	76	26	c	c	NOUN
biotropia-204	76	27	,	,	PUNCT
biotropia-204	76	28	the	the	DET
biotropia-204	76	29	optimum	optimum	ADJ
biotropia-204	76	30	activity	activity	NOUN
biotropia-204	76	31	was	be	AUX
biotropia-204	76	32	shifted	shift	VERB
biotropia-204	76	33	up	up	ADP
biotropia-204	76	34	to	to	ADP
biotropia-204	76	35	45	45	NUM
biotropia-204	76	36	°	°	NOUN
biotropia-204	76	37	c	c	NOUN
biotropia-204	76	38	due	due	ADP
biotropia-204	76	39	to	to	ADP
biotropia-204	76	40	the	the	DET
biotropia-204	76	41	presence	presence	NOUN
biotropia-204	76	42	of	of	ADP
biotropia-204	76	43	calcium	calcium	NOUN
biotropia-204	76	44	ions	ion	NOUN
biotropia-204	76	45	.	.	PUNCT
biotropia-204	77	1	thermostable	thermostable	ADJ
biotropia-204	77	2	alkaline	alkaline	NOUN
biotropia-204	77	3	protease	protease	NOUN
biotropia-204	77	4	from	from	ADP
biotropia-204	77	5	bacillus	bacillus	NOUN
biotropia-204	77	6	sp	sp	NOUN
biotropia-204	77	7	.	.	PUNCT
biotropia-204	77	8	jb-99	jb-99	NOUN
biotropia-204	77	9	reported	report	VERB
biotropia-204	77	10	by	by	ADP
biotropia-204	77	11	johnvesly	johnvesly	ADV
biotropia-204	77	12	and	and	CCONJ
biotropia-204	77	13	naik	naik	PROPN
biotropia-204	77	14	(	(	PUNCT
biotropia-204	77	15	2001	2001	NUM
biotropia-204	77	16	)	)	PUNCT
biotropia-204	77	17	had	have	VERB
biotropia-204	77	18	an	an	DET
biotropia-204	77	19	optimum	optimum	ADJ
biotropia-204	77	20	activity	activity	NOUN
biotropia-204	77	21	at	at	ADP
biotropia-204	77	22	ph	ph	PROPN
biotropia-204	77	23	11	11	NUM
biotropia-204	77	24	and	and	CCONJ
biotropia-204	77	25	70	70	NUM
biotropia-204	78	1	°	°	NOUN
biotropia-204	78	2	c	c	NOUN
biotropia-204	78	3	.	.	PUNCT
biotropia-204	79	1	this	this	DET
biotropia-204	79	2	bacterium	bacterium	NOUN
biotropia-204	79	3	was	be	AUX
biotropia-204	79	4	isolated	isolate	VERB
biotropia-204	79	5	from	from	ADP
biotropia-204	79	6	sugarcane	sugarcane	NOUN
biotropia-204	79	7	molasses	molasse	NOUN
biotropia-204	79	8	at	at	ADP
biotropia-204	79	9	ph	ph	PROPN
biotropia-204	79	10	5.8	5.8	NUM
biotropia-204	79	11	.	.	PUNCT
biotropia-204	80	1	but	but	CCONJ
biotropia-204	80	2	with	with	ADP
biotropia-204	80	3	a	a	DET
biotropia-204	80	4	chemically	chemically	ADV
biotropia-204	80	5	defined	define	VERB
biotropia-204	80	6	medium	medium	NOUN
biotropia-204	80	7	,	,	PUNCT
biotropia-204	80	8	the	the	DET
biotropia-204	80	9	bacterium	bacterium	NOUN
biotropia-204	80	10	was	be	AUX
biotropia-204	80	11	able	able	ADJ
biotropia-204	80	12	to	to	PART
biotropia-204	80	13	produce	produce	VERB
biotropia-204	80	14	alkaline	alkaline	NOUN
biotropia-204	80	15	thermostable	thermostable	NOUN
biotropia-204	80	16	protease	protease	NOUN
biotropia-204	80	17	.	.	PUNCT
biotropia-204	81	1	gessesse	gessesse	VERB
biotropia-204	81	2	et	et	PROPN
biotropia-204	81	3	al	al	PROPN
biotropia-204	81	4	.	.	PROPN
biotropia-204	82	1	(	(	PUNCT
biotropia-204	82	2	2002	2002	NUM
biotropia-204	82	3	)	)	PUNCT
biotropia-204	82	4	succeeded	succeed	VERB
biotropia-204	82	5	in	in	ADP
biotropia-204	82	6	isolating	isolate	VERB
biotropia-204	82	7	two	two	NUM
biotropia-204	82	8	bacterial	bacterial	ADJ
biotropia-204	82	9	strains	strain	NOUN
biotropia-204	82	10	from	from	ADP
biotropia-204	82	11	lake	lake	PROPN
biotropia-204	82	12	abjata	abjata	PROPN
biotropia-204	82	13	(	(	PUNCT
biotropia-204	82	14	an	an	DET
biotropia-204	82	15	alkaline	alkaline	NOUN
biotropia-204	82	16	soda	soda	NOUN
biotropia-204	82	17	lake	lake	NOUN
biotropia-204	82	18	in	in	ADP
biotropia-204	82	19	ethiopia	ethiopia	PROPN
biotropia-204	82	20	)	)	PUNCT
biotropia-204	82	21	,	,	PUNCT
biotropia-204	82	22	using	use	VERB
biotropia-204	82	23	chicken	chicken	NOUN
biotropia-204	82	24	feather	feather	NOUN
biotropia-204	82	25	meal	meal	NOUN
biotropia-204	82	26	as	as	ADP
biotropia-204	82	27	the	the	DET
biotropia-204	82	28	sole	sole	ADJ
biotropia-204	82	29	source	source	NOUN
biotropia-204	82	30	of	of	ADP
biotropia-204	82	31	nitrogen	nitrogen	NOUN
biotropia-204	82	32	and	and	CCONJ
biotropia-204	82	33	carbon	carbon	NOUN
biotropia-204	82	34	.	.	PUNCT
biotropia-204	83	1	the	the	DET
biotropia-204	83	2	bacteria	bacteria	NOUN
biotropia-204	83	3	were	be	AUX
biotropia-204	83	4	identified	identify	VERB
biotropia-204	83	5	as	as	ADP
biotropia-204	83	6	nestemkonia	nestemkonia	NOUN
biotropia-204	83	7	sp	sp	PROPN
biotropia-204	83	8	.	.	PROPN
biotropia-204	83	9	al-20	al-20	NOUN
biotropia-204	83	10	and	and	CCONJ
biotropia-204	83	11	b.	b.	PROPN
biotropia-204	83	12	pseudofirmus	pseudofirmus	PROPN
biotropia-204	83	13	al-89	al-89	PUNCT
biotropia-204	83	14	.	.	PUNCT
biotropia-204	84	1	the	the	DET
biotropia-204	84	2	optimum	optimum	ADJ
biotropia-204	84	3	enzyme	enzyme	NOUN
biotropia-204	84	4	activities	activity	NOUN
biotropia-204	84	5	of	of	ADP
biotropia-204	84	6	al-20	al-20	NOUN
biotropia-204	84	7	and	and	CCONJ
biotropia-204	84	8	al-89	al-89	ADV
biotropia-204	84	9	were	be	AUX
biotropia-204	84	10	observed	observe	VERB
biotropia-204	84	11	at	at	ADP
biotropia-204	84	12	ph	ph	PROPN
biotropia-204	84	13	10	10	NUM
biotropia-204	84	14	,	,	PUNCT
biotropia-204	84	15	70	70	NUM
biotropia-204	84	16	°	°	NOUN
biotropia-204	84	17	c	c	NOUN
biotropia-204	84	18	and	and	CCONJ
biotropia-204	84	19	at	at	ADP
biotropia-204	84	20	ph	ph	ADJ
biotropia-204	84	21	11	11	NUM
biotropia-204	84	22	,	,	PUNCT
biotropia-204	84	23	50	50	NUM
biotropia-204	84	24	°	°	NOUN
biotropia-204	84	25	c	c	NOUN
biotropia-204	84	26	,	,	PUNCT
biotropia-204	84	27	respectively	respectively	ADV
biotropia-204	84	28	.	.	PUNCT
biotropia-204	85	1	the	the	DET
biotropia-204	85	2	al-20	al-20	ADJ
biotropia-204	85	3	protease	protease	NOUN
biotropia-204	85	4	was	be	AUX
biotropia-204	85	5	calcium	calcium	NOUN
biotropia-204	85	6	independent	independent	ADJ
biotropia-204	85	7	whereas	whereas	SCONJ
biotropia-204	85	8	the	the	DET
biotropia-204	85	9	al-89	al-89	X
biotropia-204	85	10	was	be	AUX
biotropia-204	85	11	calcium	calcium	NOUN
biotropia-204	85	12	dependent	dependent	ADJ
biotropia-204	85	13	.	.	PUNCT
biotropia-204	86	1	both	both	DET
biotropia-204	86	2	enzymes	enzyme	NOUN
biotropia-204	86	3	were	be	AUX
biotropia-204	86	4	indicated	indicate	VERB
biotropia-204	86	5	as	as	ADP
biotropia-204	86	6	serine	serine	NOUN
biotropia-204	86	7	protease	protease	NOUN
biotropia-204	86	8	.	.	PUNCT
biotropia-204	87	1	bacillus	bacillus	NOUN
biotropia-204	87	2	,	,	PUNCT
biotropia-204	87	3	horikoshii	horikoshii	NOUN
biotropia-204	87	4	protease	protease	NOUN
biotropia-204	87	5	had	have	VERB
biotropia-204	87	6	optimum	optimum	ADJ
biotropia-204	87	7	activity	activity	NOUN
biotropia-204	87	8	at	at	ADP
biotropia-204	87	9	ph	ph	ADJ
biotropia-204	87	10	9	9	NUM
biotropia-204	87	11	,	,	PUNCT
biotropia-204	87	12	45"c	45"c	NUM
biotropia-204	87	13	,	,	PUNCT
biotropia-204	87	14	and	and	CCONJ
biotropia-204	87	15	the	the	DET
biotropia-204	87	16	enzyme	enzyme	NOUN
biotropia-204	87	17	was	be	AUX
biotropia-204	87	18	stable	stable	ADJ
biotropia-204	87	19	at	at	ADP
biotropia-204	87	20	the	the	DET
biotropia-204	87	21	ph	ph	ADJ
biotropia-204	87	22	range	range	NOUN
biotropia-204	87	23	of	of	ADP
biotropia-204	87	24	5.5	5.5	NUM
biotropia-204	87	25	-	-	SYM
biotropia-204	87	26	12	12	NUM
biotropia-204	87	27	and	and	CCONJ
biotropia-204	87	28	temperature	temperature	NOUN
biotropia-204	87	29	up	up	ADP
biotropia-204	87	30	to	to	PART
biotropia-204	87	31	50	50	NUM
biotropia-204	88	1	°	°	NOUN
biotropia-204	88	2	c	c	NOUN
biotropia-204	88	3	(	(	PUNCT
biotropia-204	88	4	joo	joo	PROPN
biotropia-204	88	5	et	et	PROPN
biotropia-204	88	6	al	al	PROPN
biotropia-204	88	7	,	,	PUNCT
biotropia-204	88	8	2002	2002	NUM
biotropia-204	88	9	)	)	PUNCT
biotropia-204	88	10	.	.	PUNCT
biotropia-204	89	1	effect	effect	NOUN
biotropia-204	89	2	of	of	ADP
biotropia-204	89	3	protease	protease	NOUN
biotropia-204	89	4	inhibitor	inhibitor	NOUN
biotropia-204	89	5	and	and	CCONJ
biotropia-204	89	6	divalent	divalent	NOUN
biotropia-204	89	7	ions	ion	NOUN
biotropia-204	89	8	the	the	DET
biotropia-204	89	9	presence	presence	NOUN
biotropia-204	89	10	of	of	ADP
biotropia-204	89	11	5	5	NUM
biotropia-204	89	12	mm	mm	NOUN
biotropia-204	89	13	phenyl	phenyl	NOUN
biotropia-204	89	14	methyl	methyl	NOUN
biotropia-204	89	15	sulfonyl	sulfonyl	NOUN
biotropia-204	89	16	fluoride	fluoride	NOUN
biotropia-204	89	17	(	(	PUNCT
biotropia-204	89	18	pmsf	pmsf	NOUN
biotropia-204	89	19	)	)	PUNCT
biotropia-204	89	20	reduced	reduce	VERB
biotropia-204	89	21	the	the	DET
biotropia-204	89	22	protease	protease	NOUN
biotropia-204	89	23	activity	activity	NOUN
biotropia-204	89	24	down	down	ADV
biotropia-204	89	25	to	to	ADP
biotropia-204	89	26	23.3	23.3	NUM
biotropia-204	89	27	%	%	NOUN
biotropia-204	89	28	which	which	PRON
biotropia-204	89	29	indicated	indicate	VERB
biotropia-204	89	30	the	the	DET
biotropia-204	89	31	enzyme	enzyme	NOUN
biotropia-204	89	32	belongs	belong	VERB
biotropia-204	89	33	to	to	ADP
biotropia-204	89	34	serine	serine	ADJ
biotropia-204	89	35	protease	protease	NOUN
biotropia-204	89	36	.	.	PUNCT
biotropia-204	90	1	five	five	NUM
biotropia-204	90	2	mm	mm	PROPN
biotropia-204	90	3	eot	eot	PROPN
biotropia-204	90	4	a	a	PRON
biotropia-204	90	5	almost	almost	ADV
biotropia-204	90	6	totally	totally	ADV
biotropia-204	90	7	inhibiting	inhibit	VERB
biotropia-204	90	8	the	the	DET
biotropia-204	90	9	enzyme	enzyme	NOUN
biotropia-204	90	10	with	with	ADP
biotropia-204	90	11	a	a	DET
biotropia-204	90	12	remaining	remain	VERB
biotropia-204	90	13	activity	activity	NOUN
biotropia-204	90	14	of	of	ADP
biotropia-204	90	15	7	7	NUM
biotropia-204	90	16	%	%	NOUN
biotropia-204	90	17	only	only	ADV
biotropia-204	90	18	,	,	PUNCT
biotropia-204	90	19	which	which	PRON
biotropia-204	90	20	showed	show	VERB
biotropia-204	90	21	that	that	SCONJ
biotropia-204	90	22	the	the	DET
biotropia-204	90	23	enzyme	enzyme	NOUN
biotropia-204	90	24	activity	activity	NOUN
biotropia-204	90	25	depends	depend	VERB
biotropia-204	90	26	on	on	ADP
biotropia-204	90	27	the	the	DET
biotropia-204	90	28	presence	presence	NOUN
biotropia-204	90	29	of	of	ADP
biotropia-204	90	30	metal	metal	NOUN
biotropia-204	90	31	ion	ion	NOUN
biotropia-204	90	32	.	.	PUNCT
biotropia-204	91	1	the	the	DET
biotropia-204	91	2	study	study	NOUN
biotropia-204	91	3	on	on	ADP
biotropia-204	91	4	the	the	DET
biotropia-204	91	5	effect	effect	NOUN
biotropia-204	91	6	of	of	ADP
biotropia-204	91	7	several	several	ADJ
biotropia-204	91	8	metal	metal	NOUN
biotropia-204	91	9	salts	salt	NOUN
biotropia-204	91	10	revealed	reveal	VERB
biotropia-204	91	11	that	that	SCONJ
biotropia-204	91	12	calcium	calcium	NOUN
biotropia-204	91	13	,	,	PUNCT
biotropia-204	91	14	zinc	zinc	NOUN
biotropia-204	91	15	and	and	CCONJ
biotropia-204	91	16	manganese	manganese	ADJ
biotropia-204	91	17	ion	ion	NOUN
biotropia-204	91	18	increased	increase	VERB
biotropia-204	91	19	the	the	DET
biotropia-204	91	20	activity	activity	NOUN
biotropia-204	91	21	of	of	ADP
biotropia-204	91	22	the	the	DET
biotropia-204	91	23	enzyme	enzyme	NOUN
biotropia-204	91	24	up	up	ADP
biotropia-204	91	25	to	to	PART
biotropia-204	91	26	117.2	117.2	NUM
biotropia-204	91	27	%	%	NOUN
biotropia-204	91	28	,	,	PUNCT
biotropia-204	91	29	111.3	111.3	NUM
biotropia-204	91	30	%	%	NOUN
biotropia-204	91	31	,	,	PUNCT
biotropia-204	91	32	and	and	CCONJ
biotropia-204	91	33	114.4	114.4	NUM
biotropia-204	91	34	%	%	NOUN
biotropia-204	91	35	,	,	PUNCT
biotropia-204	91	36	respectively	respectively	ADV
biotropia-204	91	37	.	.	PUNCT
biotropia-204	92	1	whereas	whereas	SCONJ
biotropia-204	92	2	,	,	PUNCT
biotropia-204	92	3	magnesium	magnesium	NOUN
biotropia-204	92	4	,	,	PUNCT
biotropia-204	92	5	ferry	ferry	NOUN
biotropia-204	92	6	and	and	CCONJ
biotropia-204	92	7	ferrous	ferrous	ADJ
biotropia-204	92	8	ions	ion	NOUN
biotropia-204	92	9	at	at	ADP
biotropia-204	92	10	5	5	NUM
biotropia-204	92	11	mm	mm	NOUN
biotropia-204	92	12	decreased	decrease	VERB
biotropia-204	92	13	the	the	DET
biotropia-204	92	14	enzyme	enzyme	NOUN
biotropia-204	92	15	activity	activity	NOUN
biotropia-204	92	16	down	down	ADV
biotropia-204	92	17	to	to	ADP
biotropia-204	92	18	91.9	91.9	NUM
biotropia-204	92	19	%	%	NOUN
biotropia-204	92	20	,	,	PUNCT
biotropia-204	92	21	64.8	64.8	NUM
biotropia-204	92	22	%	%	NOUN
biotropia-204	92	23	,	,	PUNCT
biotropia-204	92	24	and	and	CCONJ
biotropia-204	92	25	71.3	71.3	NUM
biotropia-204	92	26	%	%	NOUN
biotropia-204	92	27	,	,	PUNCT
biotropia-204	92	28	respectively	respectively	ADV
biotropia-204	92	29	.	.	PUNCT
biotropia-204	93	1	previous	previous	ADJ
biotropia-204	93	2	studies	study	NOUN
biotropia-204	93	3	on	on	ADP
biotropia-204	93	4	alkaline	alkaline	NOUN
biotropia-204	93	5	proteases	protease	NOUN
biotropia-204	93	6	reported	report	VERB
biotropia-204	93	7	that	that	SCONJ
biotropia-204	93	8	for	for	ADP
biotropia-204	93	9	maximum	maximum	ADJ
biotropia-204	93	10	activity	activity	NOUN
biotropia-204	93	11	,	,	PUNCT
biotropia-204	93	12	some	some	DET
biotropia-204	93	13	serine	serine	ADJ
biotropia-204	93	14	alkaline	alkaline	NOUN
biotropia-204	93	15	proteases	protease	NOUN
biotropia-204	93	16	were	be	AUX
biotropia-204	93	17	dependent	dependent	ADJ
biotropia-204	93	18	on	on	ADP
biotropia-204	93	19	divalent	divalent	ADJ
biotropia-204	93	20	ion	ion	NOUN
biotropia-204	93	21	such	such	ADJ
biotropia-204	93	22	as	as	ADP
biotropia-204	93	23	calcium	calcium	NOUN
biotropia-204	93	24	,	,	PUNCT
biotropia-204	93	25	magnesium	magnesium	NOUN
biotropia-204	93	26	and	and	CCONJ
biotropia-204	93	27	manganese	manganese	NOUN
biotropia-204	93	28	(	(	PUNCT
biotropia-204	93	29	kumar	kumar	PROPN
biotropia-204	93	30	&	&	CCONJ
biotropia-204	93	31	takagi	takagi	PROPN
biotropia-204	93	32	,	,	PUNCT
biotropia-204	93	33	1998	1998	NUM
biotropia-204	93	34	)	)	PUNCT
biotropia-204	93	35	.	.	PUNCT
biotropia-204	94	1	however	however	ADV
biotropia-204	94	2	,	,	PUNCT
biotropia-204	94	3	gessesse	gessesse	VERB
biotropia-204	94	4	et	et	PROPN
biotropia-204	94	5	al	al	PROPN
biotropia-204	94	6	(	(	PUNCT
biotropia-204	94	7	2002	2002	NUM
biotropia-204	94	8	)	)	PUNCT
biotropia-204	94	9	reported	report	VERB
biotropia-204	94	10	that	that	SCONJ
biotropia-204	94	11	serine	serine	NOUN
biotropia-204	94	12	alkaline	alkaline	NOUN
biotropia-204	94	13	protease	protease	NOUN
biotropia-204	94	14	activity	activity	NOUN
biotropia-204	94	15	produced	produce	VERB
biotropia-204	94	16	by	by	ADP
biotropia-204	94	17	nesternkonia	nesternkonia	NOUN
biotropia-204	94	18	sp	sp	PROPN
biotropia-204	94	19	was	be	AUX
biotropia-204	94	20	not	not	PART
biotropia-204	94	21	dependent	dependent	ADJ
biotropia-204	94	22	on	on	ADP
biotropia-204	94	23	the	the	DET
biotropia-204	94	24	presence	presence	NOUN
biotropia-204	94	25	of	of	ADP
biotropia-204	94	26	calcium	calcium	NOUN
biotropia-204	94	27	ion	ion	NOUN
biotropia-204	94	28	.	.	PUNCT
biotropia-204	95	1	the	the	DET
biotropia-204	95	2	enzyme	enzyme	NOUN
biotropia-204	95	3	was	be	AUX
biotropia-204	95	4	not	not	PART
biotropia-204	95	5	affected	affect	VERB
biotropia-204	95	6	by	by	ADP
biotropia-204	95	7	pmsf	pmsf	NOUN
biotropia-204	95	8	,	,	PUNCT
biotropia-204	95	9	but	but	CCONJ
biotropia-204	95	10	,	,	PUNCT
biotropia-204	95	11	it	it	PRON
biotropia-204	95	12	was	be	AUX
biotropia-204	95	13	inhibited	inhibit	VERB
biotropia-204	95	14	by	by	ADP
biotropia-204	95	15	another	another	DET
biotropia-204	95	16	serine	serine	ADJ
biotropia-204	95	17	protease	protease	NOUN
biotropia-204	95	18	inhibitor	inhibitor	NOUN
biotropia-204	95	19	3,4	3,4	NUM
biotropia-204	95	20	-	-	NUM
biotropia-204	95	21	dichlorocoumarine	dichlorocoumarine	NOUN
biotropia-204	95	22	at	at	ADP
biotropia-204	95	23	concentration	concentration	NOUN
biotropia-204	95	24	of	of	ADP
biotropia-204	95	25	100	100	NUM
biotropia-204	95	26	(	(	PUNCT
biotropia-204	95	27	j.m	j.m	PROPN
biotropia-204	95	28	,	,	PUNCT
biotropia-204	95	29	.	.	PUNCT
biotropia-204	96	1	36	36	NUM
biotropia-204	96	2	isolation	isolation	NOUN
biotropia-204	96	3	and	and	CCONJ
biotropia-204	96	4	selection	selection	NOUN
biotropia-204	96	5	of	of	ADP
biotropia-204	96	6	alkaline	alkaline	ADJ
biotropia-204	96	7	proteolytic	proteolytic	ADJ
biotropia-204	96	8	bacteria	bacteria	NOUN
biotropia-204	96	9	budiasih	budiasih	VERB
biotropia-204	96	10	wahyuntari	wahyuntari	PROPN
biotropia-204	96	11	et	et	PROPN
biotropia-204	96	12	al	al	PROPN
biotropia-204	96	13	.	.	PROPN
biotropia-204	96	14	control	control	PROPN
biotropia-204	96	15	edta	edta	PROPN
biotropia-204	96	16	pmsf	pmsf	NOUN
biotropia-204	96	17	mg2	mg2	PROPN
biotropia-204	96	18	+	+	PROPN
biotropia-204	96	19	ca2	ca2	PROPN
biotropia-204	96	20	+	+	PROPN
biotropia-204	96	21	zn	zn	PROPN
biotropia-204	96	22	2	2	NUM
biotropia-204	96	23	+	+	NUM
biotropia-204	96	24	mn	mn	PROPN
biotropia-204	96	25	2	2	NUM
biotropia-204	96	26	+	+	NUM
biotropia-204	96	27	fe	fe	NOUN
biotropia-204	96	28	3	3	NUM
biotropia-204	96	29	+	+	NUM
biotropia-204	96	30	fe	fe	X
biotropia-204	96	31	2	2	NUM
biotropia-204	96	32	+	+	NOUN
biotropia-204	96	33	inhibitor	inhibitor	NOUN
biotropia-204	96	34	/	/	SYM
biotropia-204	96	35	cation	cation	NOUN
biotropia-204	96	36	d	d	NOUN
biotropia-204	96	37	:	:	PUNCT
biotropia-204	96	38	2	2	NUM
biotropia-204	96	39	mm	mm	NOUN
biotropia-204	96	40	,	,	PUNCT
biotropia-204	96	41	•	•	NUM
biotropia-204	96	42	:	:	SYM
biotropia-204	96	43	5	5	NUM
biotropia-204	96	44	mm	mm	NOUN
biotropia-204	96	45	figure	figure	NOUN
biotropia-204	96	46	6	6	NUM
biotropia-204	96	47	.	.	PUNCT
biotropia-204	97	1	et'fecl	et'fecl	PROPN
biotropia-204	97	2	of	of	ADP
biotropia-204	97	3	protease	protease	NOUN
biotropia-204	97	4	inhibitor	inhibitor	NOUN
biotropia-204	97	5	and	and	CCONJ
biotropia-204	97	6	cation	cation	NOUN
biotropia-204	97	7	on	on	ADP
biotropia-204	97	8	enzyme	enzyme	NOUN
biotropia-204	97	9	activity	activity	NOUN
biotropia-204	97	10	molecular	molecular	ADJ
biotropia-204	97	11	weight	weight	NOUN
biotropia-204	97	12	of	of	ADP
biotropia-204	97	13	the	the	DET
biotropia-204	97	14	enzyme	enzyme	NOUN
biotropia-204	97	15	zymogram	zymogram	NOUN
biotropia-204	97	16	analysis	analysis	NOUN
biotropia-204	97	17	revealed	reveal	VERB
biotropia-204	97	18	that	that	SCONJ
biotropia-204	97	19	the	the	DET
biotropia-204	97	20	molecular	molecular	ADJ
biotropia-204	97	21	weight	weight	NOUN
biotropia-204	97	22	of	of	ADP
biotropia-204	97	23	the	the	DET
biotropia-204	97	24	enzyme	enzyme	NOUN
biotropia-204	97	25	was	be	AUX
biotropia-204	97	26	approximately	approximately	ADV
biotropia-204	97	27	42.8kd	42.8kd	NUM
biotropia-204	97	28	(	(	PUNCT
biotropia-204	97	29	figure	figure	NOUN
biotropia-204	97	30	7	7	NUM
biotropia-204	97	31	)	)	PUNCT
biotropia-204	97	32	.	.	PUNCT
biotropia-204	98	1	that	that	DET
biotropia-204	98	2	molecular	molecular	ADJ
biotropia-204	98	3	weight	weight	NOUN
biotropia-204	98	4	of	of	ADP
biotropia-204	98	5	most	most	ADJ
biotropia-204	98	6	bacillus	bacillus	NOUN
biotropia-204	98	7	alkaline	alkaline	NOUN
biotropia-204	98	8	proteases	protease	NOUN
biotropia-204	98	9	reported	report	VERB
biotropia-204	98	10	ranges	range	NOUN
biotropia-204	98	11	from	from	ADP
biotropia-204	98	12	15	15	NUM
biotropia-204	98	13	to	to	PART
biotropia-204	98	14	45	45	NUM
biotropia-204	98	15	kd	kd	PROPN
biotropia-204	98	16	(	(	PUNCT
biotropia-204	98	17	kumar	kumar	PROPN
biotropia-204	98	18	&	&	CCONJ
biotropia-204	98	19	takagi	takagi	PROPN
biotropia-204	98	20	,	,	PUNCT
biotropia-204	98	21	1998	1998	NUM
biotropia-204	98	22	)	)	PUNCT
biotropia-204	98	23	.	.	PUNCT
biotropia-204	99	1	a	a	DET
biotropia-204	99	2	more	more	ADV
biotropia-204	99	3	recent	recent	ADJ
biotropia-204	99	4	report	report	NOUN
biotropia-204	99	5	by	by	ADP
biotropia-204	99	6	singh	singh	PROPN
biotropia-204	99	7	et	et	PROPN
biotropia-204	99	8	al	al	PROPN
biotropia-204	99	9	(	(	PUNCT
biotropia-204	99	10	2001	2001	NUM
biotropia-204	99	11	)	)	PUNCT
biotropia-204	99	12	also	also	ADV
biotropia-204	99	13	stated	state	VERB
biotropia-204	99	14	that	that	SCONJ
biotropia-204	99	15	the	the	DET
biotropia-204	99	16	molecular	molecular	ADJ
biotropia-204	99	17	weight	weight	NOUN
biotropia-204	99	18	of	of	ADP
biotropia-204	99	19	serine	serine	ADJ
biotropia-204	99	20	alkaline	alkaline	NOUN
biotropia-204	99	21	protease	protease	NOUN
biotropia-204	99	22	produced	produce	VERB
biotropia-204	99	23	by	by	ADP
biotropia-204	99	24	bacillus	bacillus	NOUN
biotropia-204	99	25	sp	sp	ADP
biotropia-204	99	26	ssr1	ssr1	PROPN
biotropia-204	99	27	was	be	AUX
biotropia-204	99	28	29kd	29kd	ADJ
biotropia-204	99	29	which	which	PRON
biotropia-204	99	30	was	be	AUX
biotropia-204	99	31	also	also	ADV
biotropia-204	99	32	lower	low	ADJ
biotropia-204	99	33	than	than	ADP
biotropia-204	99	34	the	the	DET
biotropia-204	99	35	previous	previous	ADJ
biotropia-204	99	36	reports	report	NOUN
biotropia-204	99	37	.	.	PUNCT
biotropia-204	100	1	figure	figure	VERB
biotropia-204	100	2	7	7	NUM
biotropia-204	100	3	.	.	PUNCT
biotropia-204	100	4	zymogram	zymogram	NOUN
biotropia-204	100	5	otisolate	otisolate	ADJ
biotropia-204	100	6	d2	d2	PROPN
biotropia-204	100	7	protease	protease	NOUN
biotropia-204	100	8	37	37	NUM
biotropia-204	100	9	biotropia	biotropia	NOUN
biotropia-204	100	10	no	no	NOUN
biotropia-204	100	11	.	.	PROPN
biotropia-204	100	12	22	22	NUM
biotropia-204	100	13	,	,	PUNCT
biotropia-204	100	14	2004	2004	NUM
biotropia-204	100	15	conclusion	conclusion	NOUN
biotropia-204	100	16	in	in	ADP
biotropia-204	100	17	this	this	DET
biotropia-204	100	18	exploration	exploration	NOUN
biotropia-204	100	19	of	of	ADP
biotropia-204	100	20	protease	protease	NOUN
biotropia-204	100	21	-	-	PUNCT
biotropia-204	100	22	producing	produce	VERB
biotropia-204	100	23	bacteria	bacteria	NOUN
biotropia-204	100	24	from	from	ADP
biotropia-204	100	25	leather	leather	NOUN
biotropia-204	100	26	processing	processing	NOUN
biotropia-204	100	27	waste	waste	NOUN
biotropia-204	100	28	,	,	PUNCT
biotropia-204	100	29	only	only	ADV
biotropia-204	100	30	few	few	ADJ
biotropia-204	100	31	bacteria	bacteria	NOUN
biotropia-204	100	32	that	that	PRON
biotropia-204	100	33	produce	produce	VERB
biotropia-204	100	34	alkaloprotease	alkaloprotease	NOUN
biotropia-204	100	35	were	be	AUX
biotropia-204	100	36	discovered	discover	VERB
biotropia-204	100	37	.	.	PUNCT
biotropia-204	101	1	among	among	ADP
biotropia-204	101	2	the	the	DET
biotropia-204	101	3	three	three	NUM
biotropia-204	101	4	isolates	isolate	NOUN
biotropia-204	101	5	screened	screen	VERB
biotropia-204	101	6	.	.	PUNCT
biotropia-204	102	1	isolate	isolate	PROPN
biotropia-204	102	2	d2	d2	PROPN
biotropia-204	102	3	produced	produce	VERB
biotropia-204	102	4	the	the	DET
biotropia-204	102	5	highest	high	ADJ
biotropia-204	102	6	protease	protease	NOUN
biotropia-204	102	7	in	in	ADP
biotropia-204	102	8	submerged	submerge	VERB
biotropia-204	102	9	culture	culture	NOUN
biotropia-204	102	10	medium	medium	NOUN
biotropia-204	102	11	.	.	PUNCT
biotropia-204	103	1	based	base	VERB
biotropia-204	103	2	on	on	ADP
biotropia-204	103	3	physical	physical	ADJ
biotropia-204	103	4	and	and	CCONJ
biotropia-204	103	5	biochemical	biochemical	ADJ
biotropia-204	103	6	properties	property	NOUN
biotropia-204	103	7	of	of	ADP
biotropia-204	103	8	the	the	DET
biotropia-204	103	9	isolate	isolate	NOUN
biotropia-204	103	10	,	,	PUNCT
biotropia-204	103	11	d2	d2	PROPN
biotropia-204	103	12	most	most	ADV
biotropia-204	103	13	likely	likely	ADV
biotropia-204	103	14	belongs	belong	VERB
biotropia-204	103	15	to	to	ADP
biotropia-204	103	16	bacillus	bacillus	NOUN
biotropia-204	103	17	sp	sp	NOUN
biotropia-204	103	18	.	.	PUNCT
biotropia-204	104	1	the	the	DET
biotropia-204	104	2	optimum	optimum	ADJ
biotropia-204	104	3	activity	activity	NOUN
biotropia-204	104	4	of	of	ADP
biotropia-204	104	5	the	the	DET
biotropia-204	104	6	enzyme	enzyme	NOUN
biotropia-204	104	7	produced	produce	VERB
biotropia-204	104	8	was	be	AUX
biotropia-204	104	9	observed	observe	VERB
biotropia-204	104	10	at	at	ADP
biotropia-204	104	11	ph	ph	ADJ
biotropia-204	104	12	8	8	NUM
biotropia-204	104	13	,	,	PUNCT
biotropia-204	104	14	60	60	NUM
biotropia-204	104	15	°	°	NUM
biotropia-204	104	16	c	c	NOUN
biotropia-204	104	17	.	.	PUNCT
biotropia-204	105	1	the	the	DET
biotropia-204	105	2	enzyme	enzyme	NOUN
biotropia-204	105	3	retained	retain	VERB
biotropia-204	105	4	70	70	NUM
biotropia-204	105	5	%	%	NOUN
biotropia-204	105	6	of	of	ADP
biotropia-204	105	7	its	its	PRON
biotropia-204	105	8	activity	activity	NOUN
biotropia-204	105	9	at	at	ADP
biotropia-204	105	10	the	the	DET
biotropia-204	105	11	range	range	NOUN
biotropia-204	105	12	ph	ph	NOUN
biotropia-204	105	13	of	of	ADP
biotropia-204	105	14	7	7	NUM
biotropia-204	105	15	-	-	SYM
biotropia-204	105	16	10	10	NUM
biotropia-204	105	17	.	.	PUNCT
biotropia-204	106	1	at	at	ADP
biotropia-204	106	2	ph	ph	PROPN
biotropia-204	106	3	8	8	NUM
biotropia-204	106	4	,	,	PUNCT
biotropia-204	106	5	the	the	DET
biotropia-204	106	6	enzyme	enzyme	NOUN
biotropia-204	106	7	also	also	ADV
biotropia-204	106	8	retained	retain	VERB
biotropia-204	106	9	more	more	ADJ
biotropia-204	106	10	than	than	ADP
biotropia-204	106	11	70	70	NUM
biotropia-204	106	12	%	%	NOUN
biotropia-204	106	13	of	of	ADP
biotropia-204	106	14	its	its	PRON
biotropia-204	106	15	activity	activity	NOUN
biotropia-204	106	16	at	at	ADP
biotropia-204	106	17	the	the	DET
biotropia-204	106	18	range	range	NOUN
biotropia-204	106	19	temperature	temperature	NOUN
biotropia-204	106	20	of	of	ADP
biotropia-204	106	21	52	52	NUM
biotropia-204	106	22	-	-	SYM
biotropia-204	106	23	62	62	NUM
biotropia-204	106	24	°	°	NOUN
biotropia-204	106	25	c	c	NOUN
biotropia-204	106	26	.	.	PUNCT
biotropia-204	107	1	the	the	DET
biotropia-204	107	2	enzyme	enzyme	NOUN
biotropia-204	107	3	was	be	AUX
biotropia-204	107	4	identified	identify	VERB
biotropia-204	107	5	as	as	ADP
biotropia-204	107	6	serine	serine	NOUN
biotropia-204	107	7	protease	protease	NOUN
biotropia-204	107	8	and	and	CCONJ
biotropia-204	107	9	its	its	PRON
biotropia-204	107	10	activity	activity	NOUN
biotropia-204	107	11	was	be	AUX
biotropia-204	107	12	affected	affect	VERB
biotropia-204	107	13	by	by	ADP
biotropia-204	107	14	the	the	DET
biotropia-204	107	15	presence	presence	NOUN
biotropia-204	107	16	of	of	ADP
biotropia-204	107	17	calcium	calcium	NOUN
biotropia-204	107	18	,	,	PUNCT
biotropia-204	107	19	zink	zink	NOUN
biotropia-204	107	20	and	and	CCONJ
biotropia-204	107	21	manganese	manganese	ADJ
biotropia-204	107	22	ion	ion	NOUN
biotropia-204	107	23	.	.	PUNCT
biotropia-204	108	1	the	the	DET
biotropia-204	108	2	molecular	molecular	ADJ
biotropia-204	108	3	weight	weight	NOUN
biotropia-204	108	4	of	of	ADP
biotropia-204	108	5	the	the	DET
biotropia-204	108	6	enzyme	enzyme	NOUN
biotropia-204	108	7	as	as	SCONJ
biotropia-204	108	8	determined	determined	ADJ
biotropia-204	108	9	using	use	VERB
biotropia-204	108	10	zymogram	zymogram	NOUN
biotropia-204	108	11	analysis	analysis	NOUN
biotropia-204	108	12	was	be	AUX
biotropia-204	108	13	approximately	approximately	ADV
biotropia-204	108	14	42.8	42.8	NUM
biotropia-204	108	15	kd	kd	NOUN
biotropia-204	108	16	.	.	PUNCT
biotropia-204	109	1	further	further	ADJ
biotropia-204	109	2	investigation	investigation	NOUN
biotropia-204	109	3	is	be	AUX
biotropia-204	109	4	needed	need	VERB
biotropia-204	109	5	to	to	PART
biotropia-204	109	6	identify	identify	VERB
biotropia-204	109	7	the	the	DET
biotropia-204	109	8	isolate	isolate	NOUN
biotropia-204	109	9	using	use	VERB
biotropia-204	109	10	more	more	ADV
biotropia-204	109	11	reliable	reliable	ADJ
biotropia-204	109	12	means	mean	NOUN
biotropia-204	109	13	such	such	ADJ
biotropia-204	109	14	as	as	ADP
biotropia-204	109	15	16rrna	16rrna	NUM
biotropia-204	109	16	sequencing	sequence	VERB
biotropia-204	109	17	the	the	DET
biotropia-204	109	18	enzyme	enzyme	NOUN
biotropia-204	109	19	can	can	AUX
biotropia-204	109	20	be	be	AUX
biotropia-204	109	21	used	use	VERB
biotropia-204	109	22	in	in	ADP
biotropia-204	109	23	a	a	DET
biotropia-204	109	24	condition	condition	NOUN
biotropia-204	109	25	that	that	PRON
biotropia-204	109	26	is	be	AUX
biotropia-204	109	27	slightly	slightly	ADV
biotropia-204	109	28	alkaline	alkaline	NOUN
biotropia-204	109	29	at	at	ADP
biotropia-204	109	30	around	around	ADP
biotropia-204	109	31	ph	ph	NOUN
biotropia-204	109	32	8	8	NUM
biotropia-204	109	33	and	and	CCONJ
biotropia-204	109	34	at	at	ADP
biotropia-204	109	35	50	50	NUM
biotropia-204	109	36	-	-	SYM
biotropia-204	109	37	60	60	NUM
biotropia-204	109	38	°	°	NOUN
biotropia-204	109	39	c	c	NOUN
biotropia-204	109	40	.	.	PUNCT
biotropia-204	110	1	however	however	ADV
biotropia-204	110	2	,	,	PUNCT
biotropia-204	110	3	to	to	PART
biotropia-204	110	4	find	find	VERB
biotropia-204	110	5	suitable	suitable	ADJ
biotropia-204	110	6	application	application	NOUN
biotropia-204	110	7	of	of	ADP
biotropia-204	110	8	the	the	DET
biotropia-204	110	9	enzyme	enzyme	NOUN
biotropia-204	110	10	,	,	PUNCT
biotropia-204	110	11	other	other	ADJ
biotropia-204	110	12	considerations	consideration	NOUN
biotropia-204	110	13	have	have	VERB
biotropia-204	110	14	to	to	PART
biotropia-204	110	15	be	be	AUX
biotropia-204	110	16	taken	take	VERB
biotropia-204	110	17	into	into	ADP
biotropia-204	110	18	account	account	NOUN
biotropia-204	110	19	such	such	ADJ
biotropia-204	110	20	as	as	ADP
biotropia-204	110	21	type	type	NOUN
biotropia-204	110	22	of	of	ADP
biotropia-204	110	23	substrate	substrate	NOUN
biotropia-204	110	24	of	of	ADP
biotropia-204	110	25	the	the	DET
biotropia-204	110	26	respected	respect	VERB
biotropia-204	110	27	process	process	NOUN
biotropia-204	110	28	.	.	PUNCT
biotropia-204	111	1	hence	hence	ADV
biotropia-204	111	2	,	,	PUNCT
biotropia-204	111	3	it	it	PRON
biotropia-204	111	4	is	be	AUX
biotropia-204	111	5	necessary	necessary	ADJ
biotropia-204	111	6	to	to	PART
biotropia-204	111	7	investigate	investigate	VERB
biotropia-204	111	8	different	different	ADJ
biotropia-204	111	9	kinds	kind	NOUN
biotropia-204	111	10	of	of	ADP
biotropia-204	111	11	protein	protein	NOUN
biotropia-204	111	12	substrates	substrate	NOUN
biotropia-204	111	13	such	such	ADJ
biotropia-204	111	14	as	as	ADP
biotropia-204	111	15	hemoglobin	hemoglobin	NOUN
biotropia-204	111	16	,	,	PUNCT
biotropia-204	111	17	collagen	collagen	NOUN
biotropia-204	111	18	,	,	PUNCT
biotropia-204	111	19	gelatin	gelatin	NOUN
biotropia-204	111	20	,	,	PUNCT
biotropia-204	111	21	keratin	keratin	NOUN
biotropia-204	111	22	etc	etc	X
biotropia-204	111	23	.	.	X
biotropia-204	111	24	selecting	select	VERB
biotropia-204	111	25	the	the	DET
biotropia-204	111	26	best	good	ADJ
biotropia-204	111	27	medium	medium	NOUN
biotropia-204	111	28	to	to	PART
biotropia-204	111	29	produce	produce	VERB
biotropia-204	111	30	the	the	DET
biotropia-204	111	31	enzyme	enzyme	NOUN
biotropia-204	111	32	is	be	AUX
biotropia-204	111	33	also	also	ADV
biotropia-204	111	34	necessary	necessary	ADJ
biotropia-204	111	35	,	,	PUNCT
biotropia-204	111	36	as	as	ADV
biotropia-204	111	37	well	well	ADV
biotropia-204	111	38	as	as	ADP
biotropia-204	111	39	more	more	ADV
biotropia-204	111	40	thorough	thorough	ADJ
biotropia-204	111	41	characterization	characterization	NOUN
biotropia-204	111	42	of	of	ADP
biotropia-204	111	43	the	the	DET
biotropia-204	111	44	enzyme	enzyme	NOUN
biotropia-204	111	45	such	such	ADJ
biotropia-204	111	46	as	as	ADP
biotropia-204	111	47	the	the	DET
biotropia-204	111	48	stability	stability	NOUN
biotropia-204	111	49	of	of	ADP
biotropia-204	111	50	the	the	DET
biotropia-204	111	51	enzyme	enzyme	NOUN
biotropia-204	111	52	toward	toward	ADP
biotropia-204	111	53	ph	ph	ADJ
biotropia-204	111	54	,	,	PUNCT
biotropia-204	111	55	temperature	temperature	NOUN
biotropia-204	111	56	,	,	PUNCT
biotropia-204	111	57	and	and	CCONJ
biotropia-204	111	58	some	some	DET
biotropia-204	111	59	additives	additive	NOUN
biotropia-204	111	60	that	that	PRON
biotropia-204	111	61	might	might	AUX
biotropia-204	111	62	be	be	AUX
biotropia-204	111	63	used	use	VERB
biotropia-204	111	64	in	in	ADP
biotropia-204	111	65	enzyme	enzyme	NOUN
biotropia-204	111	66	application	application	NOUN
biotropia-204	111	67	.	.	PUNCT
biotropia-204	112	1	references	reference	NOUN
biotropia-204	112	2	banerjee	banerjee	PROPN
biotropia-204	112	3	uc	uc	PROPN
biotropia-204	112	4	,	,	PUNCT
biotropia-204	112	5	rk	rk	PROPN
biotropia-204	112	6	sani	sani	PROPN
biotropia-204	112	7	,	,	PUNCT
biotropia-204	112	8	w	w	PROPN
biotropia-204	112	9	azmi	azmi	PROPN
biotropia-204	112	10	and	and	CCONJ
biotropia-204	112	11	r	r	PROPN
biotropia-204	112	12	soni	soni	PROPN
biotropia-204	112	13	.	.	PUNCT
biotropia-204	113	1	1999	1999	NUM
biotropia-204	113	2	.	.	PUNCT
biotropia-204	114	1	thermostable	thermostable	ADJ
biotropia-204	114	2	alkaline	alkaline	NOUN
biotropia-204	114	3	protease	protease	NOUN
biotropia-204	114	4	from	from	ADP
biotropia-204	114	5	bacillus	bacillus	NOUN
biotropia-204	114	6	brevi.t	brevi.t	NOUN
biotropia-204	114	7	and	and	CCONJ
biotropia-204	114	8	its	its	PRON
biotropia-204	114	9	characterization	characterization	NOUN
biotropia-204	114	10	as	as	ADP
biotropia-204	114	11	a	a	DET
biotropia-204	114	12	laundry	laundry	ADJ
biotropia-204	114	13	detergent	detergent	NOUN
biotropia-204	114	14	additive	additive	NOUN
biotropia-204	114	15	,	,	PUNCT
biotropia-204	114	16	process	process	NOUN
biotropia-204	114	17	biochem	biochem	NOUN
biotropia-204	114	18	.	.	PUNCT
biotropia-204	115	1	35:213	35:213	NUM
biotropia-204	115	2	-	-	SYM
biotropia-204	115	3	219	219	NUM
biotropia-204	115	4	.	.	PUNCT
biotropia-204	116	1	gessesse	gessesse	VERB
biotropia-204	116	2	a	a	DET
biotropia-204	116	3	,	,	PUNCT
biotropia-204	116	4	hk	hk	PROPN
biotropia-204	116	5	rajni	rajni	PROPN
biotropia-204	116	6	,	,	PUNCT
biotropia-204	116	7	ba	ba	PROPN
biotropia-204	116	8	gashe	gashe	PROPN
biotropia-204	116	9	arid	arid	PROPN
biotropia-204	117	1	b	b	PROPN
biotropia-204	117	2	mattiasson	mattiasson	NOUN
biotropia-204	117	3	.	.	PUNCT
biotropia-204	118	1	2002	2002	NUM
biotropia-204	118	2	.	.	PUNCT
biotropia-204	119	1	novel	novel	ADJ
biotropia-204	119	2	alkaline	alkaline	NOUN
biotropia-204	119	3	proteases	protease	NOUN
biotropia-204	119	4	from	from	ADP
biotropia-204	119	5	alkaliphilic	alkaliphilic	ADJ
biotropia-204	119	6	bacteria	bacteria	NOUN
biotropia-204	119	7	grown	grow	VERB
biotropia-204	119	8	on	on	ADP
biotropia-204	119	9	chicken	chicken	NOUN
biotropia-204	119	10	feather	feather	NOUN
biotropia-204	119	11	.	.	PUNCT
biotropia-204	120	1	enzyme	enzyme	NOUN
biotropia-204	120	2	&	&	CCONJ
biotropia-204	120	3	microbial	microbial	ADJ
biotropia-204	120	4	tech	tech	NOUN
biotropia-204	120	5	.	.	PUNCT
biotropia-204	121	1	6250:1	6250:1	NUM
biotropia-204	121	2	-	-	SYM
biotropia-204	121	3	6	6	NUM
biotropia-204	121	4	.	.	PUNCT
biotropia-204	122	1	horikoshi	horikoshi	PROPN
biotropia-204	122	2	,	,	PUNCT
biotropia-204	122	3	k	k	NOUN
biotropia-204	122	4	,	,	PUNCT
biotropia-204	122	5	1971	1971	NUM
biotropia-204	122	6	.	.	PUNCT
biotropia-204	123	1	production	production	NOUN
biotropia-204	123	2	of	of	ADP
biotropia-204	123	3	alkaline	alkaline	NOUN
biotropia-204	123	4	enzymes	enzyme	NOUN
biotropia-204	123	5	by	by	ADP
biotropia-204	123	6	alkalophilic	alkalophilic	ADJ
biotropia-204	123	7	microorganisms	microorganism	NOUN
biotropia-204	123	8	.	.	PUNCT
biotropia-204	124	1	part	part	NOUN
biotropia-204	124	2	1	1	NUM
biotropia-204	124	3	.	.	PUNCT
biotropia-204	124	4	alkaline	alkaline	NOUN
biotropia-204	124	5	protease	protease	NOUN
biotropia-204	124	6	produced	produce	VERB
biotropia-204	124	7	by	by	ADP
biotropia-204	124	8	bacillus	bacillus	NOUN
biotropia-204	124	9	no	no	NOUN
biotropia-204	124	10	.	.	PUNCT
biotropia-204	125	1	221	221	NUM
biotropia-204	125	2	.	.	PUNCT
biotropia-204	126	1	agric	agric	PROPN
biotropia-204	126	2	.	.	PUNCT
biotropia-204	127	1	biol	biol	PROPN
biotropia-204	127	2	.	.	PUNCT
biotropia-204	128	1	chem	chem	NOUN
biotropia-204	128	2	.	.	PUNCT
biotropia-204	129	1	36:1407	36:1407	NOUN
biotropia-204	129	2	-	-	SYM
biotropia-204	129	3	1414	1414	NUM
biotropia-204	129	4	.	.	PUNCT
biotropia-204	130	1	horikoshi	horikoshi	PROPN
biotropia-204	130	2	k	k	PROPN
biotropia-204	130	3	,	,	PUNCT
biotropia-204	130	4	1996	1996	NUM
biotropia-204	130	5	.	.	PUNCT
biotropia-204	130	6	alkaliphiles	alkaliphile	VERB
biotropia-204	130	7	from	from	ADP
biotropia-204	130	8	an	an	DET
biotropia-204	130	9	industrial	industrial	ADJ
biotropia-204	130	10	point	point	NOUN
biotropia-204	130	11	of	of	ADP
biotropia-204	130	12	view	view	NOUN
biotropia-204	130	13	,	,	PUNCT
biotropia-204	130	14	eems	eem	VERB
biotropia-204	130	15	microbiol	microbiol	PROPN
biotropia-204	130	16	riev	riev	ADV
biotropia-204	130	17	,	,	PUNCT
biotropia-204	130	18	18:259	18:259	PROPN
biotropia-204	130	19	-	-	SYM
biotropia-204	130	20	270	270	NUM
biotropia-204	130	21	.	.	PUNCT
biotropia-204	131	1	johnvesly	johnvesly	ADV
biotropia-204	131	2	b.	b.	PROPN
biotropia-204	132	1	and	and	CCONJ
biotropia-204	133	1	gr	gr	PROPN
biotropia-204	133	2	naik	naik	PROPN
biotropia-204	133	3	.	.	PROPN
biotropia-204	133	4	2001	2001	NUM
biotropia-204	133	5	,	,	PUNCT
biotropia-204	134	1	studies	study	NOUN
biotropia-204	134	2	on	on	ADP
biotropia-204	134	3	production	production	NOUN
biotropia-204	134	4	of	of	ADP
biotropia-204	134	5	thermostable	thermostable	ADJ
biotropia-204	134	6	alkaline	alkaline	NOUN
biotropia-204	134	7	protease	protease	NOUN
biotropia-204	134	8	from	from	ADP
biotropia-204	134	9	thermophilic	thermophilic	ADJ
biotropia-204	134	10	bacillus	bacillus	NOUN
biotropia-204	134	11	sp	sp	ADP
biotropia-204	134	12	jb-99	jb-99	NOUN
biotropia-204	134	13	in	in	ADP
biotropia-204	134	14	a	a	DET
biotropia-204	134	15	chemically	chemically	ADV
biotropia-204	134	16	defined	define	VERB
biotropia-204	134	17	medium	medium	ADJ
biotropia-204	134	18	,	,	PUNCT
biotropia-204	134	19	process	process	NOUN
biotropia-204	134	20	biochem	biochem	NOUN
biotropia-204	134	21	.	.	PUNCT
biotropia-204	135	1	37:139	37:139	NUM
biotropia-204	135	2	-	-	SYM
biotropia-204	135	3	144	144	NUM
biotropia-204	135	4	.	.	PUNCT
biotropia-204	136	1	joo	joo	PROPN
biotropia-204	136	2	hs	hs	PROPN
biotropia-204	136	3	,	,	PUNCT
biotropia-204	136	4	sg	sg	PROPN
biotropia-204	136	5	kumar	kumar	PROPN
biotropia-204	136	6	,	,	PUNCT
biotropia-204	136	7	gc	gc	PROPN
biotropia-204	136	8	park	park	PROPN
biotropia-204	136	9	,	,	PUNCT
biotropia-204	136	10	kt	kt	PROPN
biotropia-204	136	11	kirn	kirn	PROPN
biotropia-204	136	12	,	,	PUNCT
biotropia-204	136	13	sr	sr	PROPN
biotropia-204	136	14	paik	paik	PROPN
biotropia-204	136	15	and	and	CCONJ
biotropia-204	136	16	cs	cs	PROPN
biotropia-204	136	17	chang	chang	PROPN
biotropia-204	136	18	.	.	PUNCT
biotropia-204	137	1	2002	2002	NUM
biotropia-204	137	2	.	.	PUNCT
biotropia-204	138	1	optimization	optimization	NOUN
biotropia-204	138	2	of	of	ADP
biotropia-204	138	3	the	the	DET
biotropia-204	138	4	production	production	NOUN
biotropia-204	138	5	of	of	ADP
biotropia-204	138	6	an	an	DET
biotropia-204	138	7	extracellular	extracellular	ADJ
biotropia-204	138	8	alkaline	alkaline	NOUN
biotropia-204	138	9	protease	protease	NOUN
biotropia-204	138	10	from	from	ADP
biotropia-204	138	11	bacillus	bacillus	NOUN
biotropia-204	138	12	horikoshii	horikoshii	NOUN
biotropia-204	138	13	,	,	PUNCT
biotropia-204	138	14	process	process	NOUN
biotropia-204	138	15	biochem	biochem	NOUN
biotropia-204	138	16	,	,	PUNCT
biotropia-204	138	17	38:155	38:155	NUM
biotropia-204	138	18	-	-	SYM
biotropia-204	138	19	159	159	NUM
biotropia-204	138	20	.	.	PUNCT
biotropia-204	139	1	kanekar	kanekar	PROPN
biotropia-204	139	2	pp	pp	PROPN
biotropia-204	139	3	,	,	PUNCT
biotropia-204	139	4	ss	ss	NOUN
biotropia-204	139	5	nilegaonkar	nilegaonkar	NOUN
biotropia-204	139	6	,	,	PUNCT
biotropia-204	139	7	ss	ss	DET
biotropia-204	139	8	sarnaik	sarnaik	NOUN
biotropia-204	139	9	and	and	CCONJ
biotropia-204	139	10	as	as	ADP
biotropia-204	139	11	kelkar	kelkar	NOUN
biotropia-204	139	12	.	.	PUNCT
biotropia-204	140	1	2002	2002	NUM
biotropia-204	140	2	.	.	PUNCT
biotropia-204	141	1	optimization	optimization	NOUN
biotropia-204	141	2	of	of	ADP
biotropia-204	141	3	protease	protease	NOUN
biotropia-204	141	4	activity	activity	NOUN
biotropia-204	141	5	of	of	ADP
biotropia-204	141	6	alkaliphilic	alkaliphilic	ADJ
biotropia-204	141	7	bacteria	bacteria	NOUN
biotropia-204	141	8	isolated	isolate	VERB
biotropia-204	141	9	from	from	ADP
biotropia-204	141	10	an	an	DET
biotropia-204	141	11	alkaline	alkaline	NOUN
biotropia-204	141	12	lake	lake	NOUN
biotropia-204	141	13	in	in	ADP
biotropia-204	141	14	india	india	PROPN
biotropia-204	141	15	,	,	PUNCT
biotropia-204	141	16	bioresource	bioresource	PROPN
biotropia-204	141	17	tech	tech	NOUN
biotropia-204	141	18	.	.	PUNCT
biotropia-204	142	1	85:87	85:87	NUM
biotropia-204	142	2	-	-	SYM
biotropia-204	142	3	93	93	NUM
biotropia-204	142	4	.	.	PUNCT
biotropia-204	143	1	kumar	kumar	PROPN
biotropia-204	143	2	cg	cg	PROPN
biotropia-204	143	3	,	,	PUNCT
biotropia-204	143	4	and	and	CCONJ
biotropia-204	143	5	h	h	PROPN
biotropia-204	143	6	takagi	takagi	PROPN
biotropia-204	143	7	1998	1998	NUM
biotropia-204	143	8	.	.	PUNCT
biotropia-204	144	1	microbial	microbial	ADJ
biotropia-204	144	2	alkaline	alkaline	NOUN
biotropia-204	144	3	proteases	protease	NOUN
biotropia-204	144	4	:	:	PUNCT
biotropia-204	144	5	from	from	ADP
biotropia-204	144	6	a	a	DET
biotropia-204	144	7	bioindustrial	bioindustrial	ADJ
biotropia-204	144	8	viewpoint	viewpoint	NOUN
biotropia-204	144	9	,	,	PUNCT
biotropia-204	144	10	biochem	biochem	PROPN
biotropia-204	144	11	adv	adv	PROPN
biotropia-204	144	12	,	,	PUNCT
biotropia-204	144	13	17:561	17:561	NUM
biotropia-204	144	14	-	-	SYM
biotropia-204	144	15	594	594	NUM
biotropia-204	144	16	.	.	PUNCT
biotropia-204	144	17	38	38	NUM
biotropia-204	144	18	isolation	isolation	NOUN
biotropia-204	144	19	and	and	CCONJ
biotropia-204	144	20	selection	selection	NOUN
biotropia-204	144	21	of	of	ADP
biotropia-204	144	22	alkaline	alkaline	ADJ
biotropia-204	144	23	proteolytic	proteolytic	ADJ
biotropia-204	144	24	bacteria	bacteria	NOUN
biotropia-204	144	25	budiasih	budiasih	VERB
biotropia-204	144	26	wahyuntari	wahyuntari	PROPN
biotropia-204	144	27	et	et	PROPN
biotropia-204	144	28	al	al	PROPN
biotropia-204	144	29	.	.	PROPN
biotropia-204	144	30	moreira	moreira	PROPN
biotropia-204	144	31	ka	ka	PROPN
biotropia-204	144	32	,	,	PUNCT
biotropia-204	144	33	ts	ts	ADP
biotropia-204	144	34	porto	porto	PROPN
biotropia-204	144	35	,	,	PUNCT
biotropia-204	144	36	mfs	mfs	PROPN
biotropia-204	144	37	teixeira	teixeira	PROPN
biotropia-204	144	38	,	,	PUNCT
biotropia-204	144	39	alf	alf	PROPN
biotropia-204	144	40	porto	porto	PROPN
biotropia-204	144	41	and	and	CCONJ
biotropia-204	144	42	jl	jl	PROPN
biotropia-204	144	43	lima	lima	PROPN
biotropia-204	144	44	filho	filho	PROPN
biotropia-204	144	45	.	.	PUNCT
biotropia-204	145	1	2003	2003	NUM
biotropia-204	145	2	.	.	PUNCT
biotropia-204	146	1	new	new	ADJ
biotropia-204	146	2	alkaline	alkaline	NOUN
biotropia-204	146	3	protease	protease	NOUN
biotropia-204	146	4	from	from	ADP
biotropia-204	146	5	nocardiopsis	nocardiopsis	NOUN
biotropia-204	146	6	sp	sp	ADP
biotropia-204	146	7	partial	partial	ADJ
biotropia-204	146	8	purification	purification	NOUN
biotropia-204	146	9	and	and	CCONJ
biotropia-204	146	10	characterization	characterization	NOUN
biotropia-204	146	11	,	,	PUNCT
biotropia-204	146	12	process	process	NOUN
biotropia-204	146	13	biochem.oo	biochem.oo	NOUN
biotropia-204	146	14	:	:	PUNCT
biotropia-204	146	15	1	1	NUM
biotropia-204	146	16	-	-	SYM
biotropia-204	146	17	6	6	NUM
biotropia-204	146	18	.	.	PUNCT
biotropia-204	147	1	singh	singh	PROPN
biotropia-204	147	2	j	j	PROPN
biotropia-204	147	3	,	,	PUNCT
biotropia-204	147	4	n	n	X
biotropia-204	147	5	batra	batra	NOUN
biotropia-204	147	6	,	,	PUNCT
biotropia-204	147	7	and	and	CCONJ
biotropia-204	147	8	rc	rc	PROPN
biotropia-204	147	9	sobti	sobti	PROPN
biotropia-204	147	10	2001	2001	NUM
biotropia-204	147	11	,	,	PUNCT
biotropia-204	147	12	serine	serine	ADJ
biotropia-204	147	13	alkaline	alkaline	NOUN
biotropia-204	147	14	protease	protease	NOUN
biotropia-204	147	15	from	from	ADP
biotropia-204	147	16	newly	newly	ADV
biotropia-204	147	17	isolated	isolate	VERB
biotropia-204	147	18	bacillus	bacillus	NOUN
biotropia-204	147	19	sp	sp	ADP
biotropia-204	147	20	ssr1	ssr1	PROPN
biotropia-204	147	21	,	,	PUNCT
biotropia-204	147	22	process	process	NOUN
biotropia-204	147	23	biochem	biochem	NOUN
biotropia-204	147	24	,	,	PUNCT
biotropia-204	147	25	36:781	36:781	NUM
biotropia-204	147	26	-	-	SYM
biotropia-204	147	27	785	785	NUM
biotropia-204	147	28	.	.	PUNCT
biotropia-204	148	1	taylor	taylor	PROPN
biotropia-204	148	2	mm	mm	PROPN
biotropia-204	148	3	,	,	PUNCT
biotropia-204	148	4	dg	dg	PROPN
biotropia-204	148	5	bailey	bailey	NOUN
biotropia-204	148	6	and	and	CCONJ
biotropia-204	148	7	sh	sh	PROPN
biotropia-204	148	8	feaiheller	feaiheller	NOUN
biotropia-204	148	9	.	.	PUNCT
biotropia-204	149	1	1987	1987	NUM
biotropia-204	149	2	.	.	PUNCT
biotropia-204	150	1	a	a	DET
biotropia-204	150	2	review	review	NOUN
biotropia-204	150	3	of	of	ADP
biotropia-204	150	4	the	the	DET
biotropia-204	150	5	use	use	NOUN
biotropia-204	150	6	of	of	ADP
biotropia-204	150	7	enzymes	enzyme	NOUN
biotropia-204	150	8	in	in	ADP
biotropia-204	150	9	tannery	tannery	NOUN
biotropia-204	150	10	,	,	PUNCT
biotropia-204	150	11	j.	j.	PROPN
biotropia-204	150	12	ame	ame	PROPN
biotropia-204	150	13	leather	leather	PROPN
biotropia-204	150	14	chem	chem	PROPN
biotropia-204	150	15	assoc	assoc	NOUN
biotropia-204	150	16	,	,	PUNCT
biotropia-204	150	17	82:153	82:153	NUM
biotropia-204	150	18	-	-	SYM
biotropia-204	150	19	165	165	NUM
biotropia-204	150	20	.	.	PUNCT
biotropia-204	151	1	walter	walter	PROPN
biotropia-204	151	2	he	he	PRON
biotropia-204	151	3	.	.	PUNCT
biotropia-204	152	1	1984	1984	NUM
biotropia-204	152	2	.	.	PUNCT
biotropia-204	153	1	proteinases	proteinase	NOUN
biotropia-204	153	2	in	in	ADP
biotropia-204	153	3	methods	method	NOUN
biotropia-204	153	4	of	of	ADP
biotropia-204	153	5	enzymatic	enzymatic	ADJ
biotropia-204	153	6	analysis	analysis	NOUN
biotropia-204	153	7	,	,	PUNCT
biotropia-204	153	8	(	(	PUNCT
biotropia-204	153	9	ed	ed	NOUN
biotropia-204	153	10	.	.	PUNCT
biotropia-204	154	1	h.u	h.u	NOUN
biotropia-204	154	2	.	.	PUNCT
biotropia-204	155	1	bergmeyer	bergmeyer	NOUN
biotropia-204	155	2	and	and	CCONJ
biotropia-204	155	3	m	m	PRON
biotropia-204	155	4	qrassl	qrassl	ADJ
biotropia-204	155	5	)	)	PUNCT
biotropia-204	155	6	,	,	PUNCT
biotropia-204	155	7	verlag	verlag	PROPN
biotropia-204	155	8	chemie	chemie	PROPN
biotropia-204	155	9	.	.	PUNCT
biotropia-204	155	10	weinheim	weinheim	PROPN
biotropia-204	155	11	-	-	PUNCT
biotropia-204	155	12	deerfield	deerfield	PROPN
biotropia-204	155	13	,	,	PUNCT
biotropia-204	155	14	florida	florida	PROPN
biotropia-204	155	15	-	-	PUNCT
biotropia-204	155	16	basse	basse	PROPN
biotropia-204	155	17	!	!	PROPN
biotropia-204	155	18	,	,	PUNCT
biotropia-204	155	19	vol	vol	NOUN
biotropia-204	155	20	.	.	PUNCT
biotropia-204	156	1	5:271	5:271	NUM
biotropia-204	156	2	-	-	SYM
biotropia-204	156	3	276	276	NUM
biotropia-204	156	4	.	.	PUNCT
biotropia-204	156	5	39	39	NUM
biotropia-204	157	1	29.pdf	29.pdf	PROPN
biotropia-204	157	2	30.pdf	30.pdf	PROPN
biotropia-204	157	3	31.pdf	31.pdf	PROPN
biotropia-204	157	4	32.pdf	32.pdf	PROPN
biotropia-204	157	5	33.pdf	33.pdf	PROPN
biotropia-204	157	6	34.pdf	34.pdf	PROPN
biotropia-204	157	7	35.pdf	35.pdf	PROPN
biotropia-204	157	8	36.pdf	36.pdf	PROPN
biotropia-204	157	9	37.pdf	37.pdf	PROPN
biotropia-204	157	10	38.pdf	38.pdf	PROPN
biotropia-204	157	11	39.pdf	39.pdf	PROPN