Untitled 1 Volume 16 2017 e17079 Original Article 1 Graduate student, Graduate Program in Dentistry, Universidade Positivo, Curitiba, PR, Brazil. 2 Professor, Graduate Program in Dentistry, Universidade Positivo, Curitiba, PR, Brazil. Corresponding author: Carla Castiglia Gonzaga Universidade Positivo Rua Prof. Pedro Viriato Parigot de Souza, 5300 – 81280-330 – Curitiba – PR – Brasil Phone: (5541) 3317-3180 Fax: (5541) 3317-3082 E-mail: carlacgonzaga2@gmail.com Received: July 25, 2017 Accepted: November 23, 2017 Effect of hydrogen peroxide and universal adhesive application on the bond strength of glass ƼFIV�TSWXW Fernando Dalitz, DDS, MSc1; Orides Ferrari de Oliveira Junior, DDS1; Raisa Carolina Hintz, DDS, MSc1; Gisele Maria Correr, DDS, MSc, PhD2; Leonardo Fernandes da Cunha, DDS, MSc, PhD2; Carla Castiglia Gonzaga DDS, PhD2 Aim: This study aims to evaluate the effect of different WYVJEGI�XVIEXQIRXW�SR�XLI�FSRH�WXVIRKXL�SJ�KPEWW�ƼFIV�TSWXW�� Methods: 2MRIX]� YRMVVEHMGYPEV� TVIQSPEVW� LEH� XLIMV� GVS[RW� VIQSZIH� ERH� [IVI� IRHSHSRXMGEPP]� XVIEXIH�� %JXIV� ��L�� TSWX� WTEGIW�[IVI�TVITEVIH�ERH�XLI�VSSXW��R!�� �[IVI�HMZMHIH�MRXS� WM\�KVSYTW�EGGSVHMRK�XS�XLI�TSWX�WYVJEGI�XVIEXQIRX��28�Ɓ�RS� XVIEXQIRX�� 9%� Ɓ� YRMZIVWEP� EHLIWMZI� �7MRKPI� &SRH� 9RMZIVWEP �� 79%�Ɓ�WMPERI��4VSWMP ��JSPPS[IH�F]�YRMZIVWEP�EHLIWMZI��,�Ɓ�, 2 O 2 �;LMXRIWW�,4�1E\\���� ��KIP����QMR ��,9%�Ɓ�, 2 O 2 �� JSPPS[IH� F]� YRMZIVWEP� EHLIWMZI�� ERH� ,79%� Ɓ� , 2 O 2 �� JSPPS[IH� F]� WMPERI� ERH�YRMZIVWEP�EHLIWMZI��8LI�TSWXW�[IVI�GIQIRXIH�[MXL�HYEP� GYVIH� VIWMR� GIQIRX� �6IP]<� 9PXMQEXI �� &SRH� WXVIRKXL� [EW� IZEPYEXIH�[MXL�TYPP�SYX�XIWX������QQ�QMQ �EJXIV���L�WXSVEKI� MR�HMWXMPPIH�[EXIV�EX���o'��(EXE�[IVI�WYFQMXXIH�XS�%23:%�ERH� Tukey’s test (D!� ��Results: ,79%�KVSYT�WLS[IH�XLI�LMKLIWX� FSRH�WXVIRKXL�ZEPYI��������2 �JSPPS[IH�F]�,9%��������2 ��28� �����2 �ERH�79%������2 �KVSYTW��9%�KVSYT�WLS[IH�XLI�PS[IWX� bond strength value (60 N). Conclusion:�8LI�YWI�SJ�, 2 O 2 , the WMPERI�ETTPMGEXMSR�SV�XLI�GSQFMREXMSR�SJ�XLIWI�X[S�XVIEXQIRXW� WMKRMƼGERXP]�MRGVIEWIH�FSRH�WXVIRKXL�ZEPYIW�MR�KVSYTW�MR�[LMGL� XLI�YRMZIVWEP�EHLIWMZI�[EW�YWIH��%�WMQTPMƼIH�TVSGIHYVI��WYGL� EW� GPIERMRK� [MXL� EPGSLSP�� GER� FI� VIGSQQIRHIH� EW� WYVJEGI� XVIEXQIRX�JSV�XLI�GIQIRXEXMSR�SJ�KPEWW�ƼFIV�TSWXW� Keywords:� 4SWX� ERH� GSVI� XIGLRMUYI�� ,]HVSKIR� TIVS\MHI�� Dentin-bonding agents. http://dx.doi.org/10.20396/bjos.v16i0.8651187 mailto:carlacgonzaga2@gmail.com 2 Dalitz et al. Introduction The dental restoration of endodontically treated teeth is considered one of the most GVMXMGEP�VIWXSVEXMZI�TVSGIHYVIW��HYI�XS�MXW�GSQTPI\�REXYVI�ERH�MRZSPZIQIRX�SJ�HMJJIVIRX� WTIGMEPXMIW��WYGL�EW�)RHSHSRXMGW�ERH�3TIVEXMZI�(IRXMWXV]��-R�WSQI�WMXYEXMSRW��TEVXMGY- PEVP]�[LIR�XLIVI�MW�WMKRMƼGERX�PSWW�SJ�GSVSREP�XSSXL�WXVYGXYVI��XLI�YWI�SJ�TSWX�ERH�GSVI� W]WXIQW�MW�VIGSQQIRHIH��+PEWW�ƼFIV�TSWXW�KEMRIH�TSTYPEVMX]� MR�XLI�PEWX�HIGEHIW� FIGEYWI�EVI�QSVI�ƽI\MFPI�XLI�QIXEP�TSWX�GSVIW�ERH�LEZI�IPEWXMG�QSHYPYW�WMQMPEV�XS� the dentin1��1SVISZIV��WMRGI�XLI]�GER�FI�EHLIWMZIP]�GIQIRXIH��QEWXMGEXSV]�WXVIWWIW� XIRH�XS�FI�QSVI�IZIRP]�HMWXVMFYXIH�MR�XLI�VSSX��VIWYPXMRK�MR�JI[IV�JVEGXYVIW�SV�QSVI� JEZSVEFPI�SRIW��RSX�GEXEWXVSTLMG ��[LMGL�GER�FI�WYFNIGXIH�XS�VITEMV1-3. -R�SVHIV�XS�TVSZMHI�FIXXIV�VIXIRXMSR�JSV�KPEWW�ƼFIV�TSWXW��PMXIVEXYVI�VIGSQQIRHW�YWMRK� ZEVMSYW�WYVJEGI�XVIEXQIRX�XIGLRMUYIW��IWTIGMEPP]�GPIERMRK�TSWX�WYVJEGI�[MXL�EPGSLSP�� GSRHMXMSRMRK�[MXL�TLSWTLSVMG�EGMH�SV�L]HVSƽYSVMG�EGMH��WERHFPEWX�[MXL�EPYQMRYQ�S\MHI�� WMPMGEXMPM^EXMSR�� WMPERI� ERH�SV� L]HVSTLSFMG� EHLIWMZI� ETTPMGEXMSR�� ERH�� QSVI� VIGIRXP]�� ETTPMGEXMSR�SJ�L]HVSKIR�TIVS\MHI��, 2 O 2 ) at different concentrations and times4-10. 0MXIVEXYVI�TVIWIRXW�WMKRMƼGERX�VIWYPXW�VIKEVHMRK�FSRH�WXVIRKXL�ZEPYIW�XS�HIRXMR�[LIR� KPEWW� ƼFIV� TSWXW� [IVI� IXGLIH� [MXL� L]HVSKIR� TIVS\MHI5,9,11-14�� ,]HVSKIR� TIVS\MHI� EX� GSRGIRXVEXMSRW�SJ��� �XS��� �MW�ORS[R�XS�IJJIGXMZIP]�VIQSZI�E�WYVJEGI�PE]IV�SJ�ITS\]� VIWMR�SJ�KPEWW�ƼFIV�TSWXW9��,S[IZIV��XLI�ETTPMGEXMSR�XMQI�SJ���XS����QMRYXIW�YWIH�MR� earlier studies can be considered long9,15��*I[�WXYHMIW�IZEPYEXIH�XLI�YWI�SJ�, 2 O 2 at HMJJIVIRX�GSRGIRXVEXMSRW�ERH�PS[IV�ETTPMGEXMSR�XMQIW5,8��-X�MW�EPWS�MQTSVXERX�XS�RSXI�XLEX� , 2 O 2 �MW�GSQQSRP]�YWIH�MR�HIRXEP�TVEGXMGI�JSV�XSSXL�[LMXIRMRK�ERH��XLIVIJSVI��IEWMP]� EGGIWWMFPI�JSV�YWI�EW�TSWX�WYVJEGI�XVIEXQIRX5��1SVISZIV��MX�MW�MQTSVXERX�XS�IQTLEWM^I� XLEX�XLIVI�MW�RSX�]IX�IRSYKL�MRJSVQEXMSR�EFSYX�XLI�FIWX�GSRGIRXVEXMSR�ERH�ETTPMGEXMSR� XMQI�SJ�L]HVSKIR�TIVS\MHI�MR�XLI�WYVJEGI�XVIEXQIRX�SJ�KPEWW�ƼFIV�TSWXW5,9,11,13-15. 9RMZIVWEP� EHLIWMZIW� EVI� WMQTPMƼIH� EHLIWMZI� W]WXIQW� XLEX� GER� FI� YWIH� [MXL� FSXL� ETTVSEGLIW��XSXEP��ERH�W�WIPJ�IXGLMRK �ERH��FIGEYWI�SJ�XLMW��EVI�GSRWMHIVIH�QSVI�ZIV- satile16,17. These universal adhesives differ from current self-etching systems by incor- TSVEXMRK�QSRSQIVW�GETEFPI�SJ�TVSHYGMRK�E�GLIQMGEP�FSRH�[MXL�XLI�HIRXEP�XMWWYIW17,18. ,S[IZIV��XLIVI�MW�PMXXPI�MRJSVQEXMSR�SR�XLI�TIVJSVQERGI�SJ�XLIWI�RI[�YRMZIVWEP�EHLI- sives. Chen et al.19������ �VITSVXIH�XLEX�XLI�FSRH�WXVIRKXL�SJ�E�YRMZIVWEP�EHLIWMZI�XS� dentin is not different from that of total- and self-etching adhesives. This study also WLS[IH�XLEX�XLIVI�[EW�RS�WXEXMWXMGEP�HMJJIVIRGI�FIX[IIR�XLI�QSHIW�SJ�ETTPMGEXMSR�� %�QIXE�EREP]WMW�IZEPYEXIH����WXYHMIW�MR�ER�EXXIQTX�XS�IWXEFPMWL�[LEX�XLI�FIWX�TVS- XSGSP�JSV�YRMZIVWEP�EHLIWMZIW�[EW��ERH�VITSVXIH�RS�WXEXMWXMGEPP]�WMKRMƼGERX�HMJJIVIRGI� FIX[IIR�XLI�WXVEXIKMIW��XSXEP��ERH�WIPJ�IXGLMRK ��%PWS��XLI�FSRH�WXVIRKXL�XS�IREQIP� [EW�MQTVSZIH�[MXL�TVMSV�GSRHMXMSRMRK�[MXL�TLSWTLSVMG�EGMH�YRMZIVWEP�[LIR�YRMZIVWEP� EHLIWMZIW�[IVI�YWIH20. 7MRKPI�&SRH�9RMZIVWEP�MW�ER�I\EQTPI�SJ�YRMZIVWEP�EHLIWMZI��[LMGL�EPWS�LEW�WMPERI�MR� MXW�GSQTSWMXMSR��8LMW�[SYPH�WMQTPMJ]�XLI�GPMRMGEP�WXITW��IPMQMREXMRK�XLI�RIIH�JSV�WMPERI� ETTPMGEXMSR� EW� E� WITEVEXI� WXIT� HYVMRK� VIWXSVEXMSRW� VITEMV� TVSXSGSPW�� JSV� I\EQTPI21. 7MRGI�XLI�YWI�SJ�XLMW�YRMZIVWEP�EHLIWMZI�[MXL�WMPERI�EW�WYVJEGI�XVIEXQIRX�GSYPH�MRƽY- 3 Dalitz et al. IRGI�XLI�FSRH�WXVIRKXL�SJ�GSQTSWMXIW�ERH�GIVEQMGW21��MX�GER�FI�L]TSXLIWM^IH�XLEX�MXW� YWI�GSYPH�EPWS�FI�ER�STXMSR�JSV�XLI�WYVJEGI�XVIEXQIRX�SJ�KPEWW�ƼFIV�TSWXW� 8LYW��XLI�SFNIGXMZI�SJ�XLMW�WXYH]�MW�XS�IZEPYEXI�XLI�IJJIGX�SJ�HMJJIVIRX�WYVJEGI�XVIEX- QIRXW��YRMZIVWEP�EHLIWMZI�ETTPMGEXMSR�EWWSGMEXIH�SV�RSX�XS�XLI�ETTPMGEXMSR�SJ�WMPERI�� [MXL�ERH�[MXLSYX�TVIZMSYW�YWI�SJ�L]HVSKIR�TIVS\MHI �SR�XLI�FSRH�WXVIRKXL�SJ�KPEWW� ƼFIV�TSWXW�XS�VSSX�HIRXMRI�YWMRK�XLI�TYPP�SYX�XIWX��8LI�L]TSXLIWMW�XIWXIH�MW�XLEX�XLI� HMJJIVIRX�WYVJEGI�XVIEXQIRXW�SJ�XLI�KPEWW�ƼFIV�TSWXW�[SYPH�MRGVIEWI�XLI�FSRH�WXVIRKXL� values to root dentine. 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Table 1. Mean values and standard deviations of bond-strength, according to the glass fiber surface treatment. Surface treatment Bond strength (N) No treatment (NT) 194. 0 r 54.2 ab Universal adhesive (UA) 60.0 r 18.8 c Silane and universal adhesive (SUA) 184.0 r 38.6 ab Hydrogen peroxide (H) 177.5 r 45.3 b Hydrogen peroxide and universal adhesive (HUA) 206.5 r 64.6 ab Hydrogen peroxide, silane and universal adhesive (HSUA) 236.5 r 70.1 a Different superscript letters indicate statistically significant differences among the groups (p < 0.05). 5 Dalitz et al. Discussion 8LI�L]TSXLIWMW�WXYHMIH�MR�XLI�TVIWIRX�[SVO�[EW�VINIGXIH�FIGEYWI�XLI�HMJJIVIRX�WYV- JEGI�XVIEXQIRXW�SJ�KPEWW�ƼFIV�TSWXW�MRGVIEWIH�XLI�FSRH�WXVIRKXL�ZEPYIW�XS�VSSX�HIRXMR�� *SYV�KVSYTW�WLS[IH�WXEXMWXMGEPP]�WMQMPEV�FSRH�WXVIRKXL�ZEPYIW�XS�XLI�GSRXVSP�KVSYT�� 3RP]�XLI�YRMZIVWEP�EHLIWMZI�KVSYT�WLS[IH�PS[IV�FSRH�WXVIRKXL�ZEPYIW�XLEX�[IVI�WXE- XMWXMGEPP]�HMJJIVIRX�JVSQ�XLI�SXLIV�KVSYTW�ERH�EPWS�JVSQ�GSRXVSP�KVSYT� 8LI�YWI�SJ�HMJJIVIRX�WYVJEGI�XVIEXQIRXW�MR�KPEWW�ƼFIV�TSWXW�EMQW�XS�TVSQSXI�E�FIXXIV� FSRH�FIX[IIR�XLI�TSWX�ERH�XLI�VIWMR�GIQIRXW�YWIH�JSV�PYXMRK��+PEWW�ƼFIV�TSWXW�EVI� GSZIVIH�F]�ER�ITS\]�VIWMR�PE]IV�XLEX�LEW�LMKL�HIKVII�SJ�GSRZIVWMSR�ERH�JI[�VIEGXMZI� sites for chemical adhesion to other resin materials22,23��8LMW�LMKLP]�VIXMGYPEXIH�ERH�PS[� FMRHMRK�XS�XLI�VIWMRSYW�QEXIVMEP�WXVYGXYVI�QE]�FI�GSQTIRWEXIH�F]�QMGVSQIGLERMGEP� VIXIRXMSR�SR�XLI�WYVJEGI�SJ�KPEWW�ƼFIV�TSWXW�GVIEXIH��JSV�I\EQTPI��F]�, 2 O 2 ��JSPPS[IH�F]� WMPERI�GSYTPMRK�EKIRX�ETTPMGEXMSR5,7,8��8LI�I\TSWYVI�SJ�XLI�KPEWW�ƼFIVW�ERH�XLI�ETTPMGE- XMSR�SJ�WMPERI�GSYTPMRK�EKIRX�MQTVSZI�XLI�WYVJEGI�[IXXEFMPMX]�ERH�JEGMPMXEXI�XLI�GLIQM- GEP�FSRH�FIX[IIR�ƼFIVW�ERH�VIWMRSYW�QEXIVMEPW6,7,9. %PXLSYKL�PMXIVEXYVI�VITSVXW�XLEX�HMJJIVIRX�WYVJEGI�XVIEXQIRXW�TIVJSVQIH�SR�XLI�KPEWW� ƼFIV�TSWXW�GER�MQTVSZI�FSRH�WXVIRKXL�XS�HIRXMR5,9,11-13,24��E�W]WXIQEXMG�VIZMI[�SJ�XLI�PMX- IVEXYVI�XLEX�EMQIH�XS�MHIRXMJ]�JEGXSVW�XLEX�QE]�EJJIGX�XLI�VIXIRXMSR�SJ�KPEWW�ƼFIV�TSWXW� XS�VSSX�HIRXMR�WLS[IH�XLEX�IRHSHSRXMG�XVIEXQIRX��VIWMR�GIQIRX�ETTPMGEXMSR�QIXLSH� ERH�TSWX�WYVJEGI�XVIEXQIRX�QE]�WMKRMƼGERXP]� MRƽYIRGI�XLI�VIXIRXMSR�SJ�KPEWW�ƼFIV� TSWX25��8LI�WXYH]�EPWS�MRHMGEXIH�XLEX�XLI�MRƽYIRGI�SJ�XLIWI�JEGXSVW�HITIRHW�SR�XLI� X]TI�SJ�VIWMR�GIQIRX�YWIH��ERH�XLMW�MW�QSVI�MQTSVXERX�[LIR�XLI�TSWXW�EVI�GIQIRXIH� [MXL�GSRZIRXMSREP�VIWMR�GIQIRXW��7IPJ�EHLIWMZI�GIQIRXW�[IVI�GSRWMHIVIH�PIWW�WIRWM- XMZI�XS�ZEVMEXMSRW�MR�XLI�GIQIRXEXMSR�XIGLRMUYI�[LIR�GSQTEVIH�XS�GSRZIRXMSREP�VIWMR� GIQIRXW��8LI�VIWYPXW�SJ�XLI�W]WXIQEXMG�VIZMI[�WLS[IH�XLEX��JSV�GSRZIRXMSREP�VIWMR� GIQIRXW��GPIERMRK�XLI�TSWXW�MRGVIEWIH�WMKRMƼGERXP]��F]����� �XLI�FSRH�WXVIRKXL�ZEP- YIW�[LIR�GSQTEVIH�XS�WMPERI�GSYTPMRK�EKIRX�ETTPMGEXMSR�[MXLSYX�GPIERMRK��IXLERSP�� EMV�EFVEWMSR��SV�TLSWTLSVMG�EGMH�ETTPMGEXMSR ��3R�XLI�SXLIV�LERH��GSRWMHIVMRK�SRP]�XLI� self-adhesive cements and all data (conventional and self-adhesive cements), the sur- JEGI�XVIEXQIRX�SJ�TSWXW��GPIERMRK�SV�WMPERM^EXMSR �HMH�RSX�EJJIGX�FSRH�WXVIRKXL�[LIR� GSQTEVIH�[MXL�XLI�YWI�SJ�XLIWI�X[S�XVIEXQIRXW�XSKIXLIV25. Table 2. Post surface analysis after pull-out test. Surface treatment Score 1 Score 2 NT 88.9% 11.1% UA 70% 30% SUA 75% 25% H 88.9% 11.1% HUA 81,8% 18.2% HSUA 80% 20% 6 Dalitz et al. 8LIWI�IZMHIRGIW�QE]�TVSZMHI�ER�I\TPEREXMSR�JSV�XLI�VIWYPXW�JSYRH�MR�XLI�TVIWIRX�WXYH]�� %�GSRZIRXMSREP�VIWMR�GIQIRX��6IP]�<�9PXMQEXI��XSKIXLIV�[MXL�XLI�7MRKPI�&SRH�9RMZIVWEP� EHLIWMZI�W]WXIQ �[EW�YWIH�XS�PYXI�XLI�KPEWW�ƼFIV�TSWXW�ERH�EPP�TSWXW�[IVI�GPIERIH� [MXL�EPGSLSP�FIJSVI�XLI�WYVJEGI�XVIEXQIRXW�[IVI�TIVJSVQIH��%W�WIIR�MR�8EFPI����XLI� FSRH�WXVIRKXL�ZEPYIW�JSYRH�JSV�XLI�GSRXVSP�KVSYT��28��RS�XVIEXQIRX��SRP]�GPIERMRK�[MXL� EPGSLSP �[IVI�VIPEXMZIP]�LMKL�ERH�XLI]�HMH�RSX�WLS[�WMKRMƼGERX�HMJJIVIRGIW�MR�VIPEXMSR�XS� SXLIV�KVSYTW��I\GITX�XLI�KVSYT�XLEX�YWIH�XLI�YRMZIVWEP�EHLIWMZI��9% ��8LMW�JEGX�WLS[W� XLEX�SRP]�GPIERMRK�[MXL�EPGSLSP�GER�FI�WYƾGMIRX�XS�EGLMIZI�E�KSSH�FSRH�[MXL�XLI�VIWMR� GIQIRX��[LMGL�GER�WMQTPMJ]�XLI�TVSXSGSP�XS�XVIEX�XLI�WYVJEGIW�SJ�KPEWW�ƼFIV�TSWXW� %PWS� XLMROMRK� EFSYX� XLI� WMQTPMƼGEXMSR�� YRMZIVWEP� EHLIWMZIW�� [LMGL� GER� FI� YWIH� 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