1http://dx.doi.org/10.20396/bjos.v20i00.8660971 Volume 20 2021 e210971 Original Article 1 State University of Feira de Santana, Department of Health, Feira de Santana, Bahia, Brazil. 2 São Judas Tadeu University, Department of Operative Dentistry, Sao Paulo, Sao Paulo, Brazil. 3 State University of Feira de Santana, Department of Biological Sciences, Feira de Santana, Bahia, Brazil. * Corresponding author: Ynara Bosco de Oliveira Lima-Arsati UEFS – Av. Transnordestina, s/n. Bairro Novo Horizonte, Feira de Santana, Bahia, Brasil. ZIP CODE: 44036-900. Phone/Fax: (+55) 75 3161-8019. Email: ynaralima76@gmail.com Received: August 22, 2020 Accepted: December 14, 2020 In vitro determination of potentially bioavailable fluoride in diet and toothpaste after ingestion Cristiane Brandão Santos Almeida1 , José Augusto Rodrigues2 , Valéria Souza Freitas1 , Ynara Bosco de Oliveira Lima-Arsati3,* Aim: To propose a new method to determine in vitro potentially bioavailable fluoride (F) in diet and toothpaste after ingestion. Methods: Diet samples (D) were obtained from 15 portions of a meal served to children in a day care centre. To simulate the ingestion of toothpaste during brushing after meals, a specific amount of toothpaste was added to the diet samples (D + T). F was determined in D and D + T after incubation in a solution that simulated “gastric juice” (0.01 M hydrochloric acid) at 37oC for 30, 60 and 120  min. Microdiffusion facilitated by HMDS was used to determine the total F concentrations in samples D and D + T. The analyses were performed using an ion specific electrode. Results: For D samples, incubation in “gastric juice” for 30, 60 and 120 min resulted in F concentrations (μg F/mL) of 0.75 ± 0.06c, 0.77 ± 0.07c and 0.91 ± 0.09b, corresponding to 75.3, 77.3 and 90.7% of the total F (1.02 ± 0.12a), respectively (p = 0.0001; ANOVA + Tukey). For D + T samples, these values of F concentrations (μg F/mL) were 2.55 ± 0.46b, 2.83 ± 0.44ab and 3.15 ± 0.37a, corresponding to 86.9, 94.8 and 106.7% of the total F (2.99 ± 0.34a), respectively (p = 0.0023; ANOVA + Tukey). Conclusion: Then, it can be concluded that the proposed method of “gastric juice” is a promising protocol for determining potentially bioavailable fluoride in the diet and toothpaste after ingestion. However, additional studies are desirable. Keywords: Fluoride. Dentifrice. Toothpaste. Diet. Dental Fluorosis. http://dx.doi.org/10.20396/bjos.v20i00.8660971 mailto:ynaralima76@gmail.com https://orcid.org/0000-0002-7424-1160 https://orcid.org/0000-0003-4172-1499 https://orcid.org/0000-0002-7259-4827 https://orcid.org/0000-0002-1059-2797 2 Almeida et al. Introduction Several studies were developed to estimate the effect of fluoride (F) intake and risk of dental fluorosis development. The mean intake of F from diet and toothpaste observed in scientific studies ranged from 0.036 to 0.090 mg F/kg/day1-7. However, the correla- tion between high doses of F exposure and high prevalence of dental fluorosis has not been established yet8-9. This is probably because the doses determined may be over- estimated for two reasons: the daily brushing frequency is not as high as that reported3 and the fact that the dose is based on the amount of ingested F, rather than absorbed. In vivo studies demonstrate that the presence of food in the stomach decreases the absorption of F from the toothpaste ingested during brushing after regular meals10. The reason is probably the increase in the pH of the stomach or the formation of low solubility salts between F and cations calcium (Ca2+), aluminum (Al3+) and mag- nesium (Mg2+) from food. Another aspect to consider is the type of abrasive pres- ent in the toothpaste. Toothpastes with calcium-based abrasives, such as calcium carbonate (CaCO3) have part of the insoluble F, because it is bounded to calcium. The silica-based abrasive toothpastes present the entire F in the soluble form, being bioavailable for absorption11. Thus, the reported dose values may be overestimated, as discussed in some of these studies3,8. When F is needed to be quantified in food samples using ion-specific elec- trode, it is necessary to extract the F ion (F-), since the electrode can only detect F ion in solution12-13. The recommended method to determine F in food samples is micro- diffusion facilitated by HMDS14-15, originally described by Taves14 (1968), and man- ages to extract total F of the samples, even solid ones, besides concentrating them approximately 6.7 times, which increases the sensitivity of the method. However, it is a laborious, expensive, and time-consuming technique. Considering the use of different protocols for microdiffusion, Martínez-Mier  et  al.15 (2011) demonstrated that a standardization of techniques increased the recovery of F and resulted in very precise and exact values between different laboratories. For food samples, they advocated the use of microdiffusion in a protocol which has some differences from the original technique, in addition to having the same critical step of completing the final volume of the drops with deionized water to compensate for evaporation. Although the protocols for total F extraction are established, not all F is soluble in food and cannot be absorbed. Therefore, methods that can determine potentially bioavail- able fluoride are desirable to improve the discussion about F intake and the risk of dental fluorosis16. It was reported that the use of 0.01 M HCl to dissolve samples of prenatal supplements resulted in the recovery of 38.3% of the total F17. The authors suggested that body temperature and peristaltic movements should be simulated to provide more realistic results for potentially bioavailable F during digestion. Considering that the main sources of systemic F for children at risk for dental fluo- rosis are diet (food and beverages) and toothpaste 1-7, the aim of the present study was to propose a new method to determine in vitro the concentration of potentially bioavailable F in samples of diet and toothpaste. 3 Almeida et al. Methodology Experimental design This was an in vitro study. The experimental units were 15 diet samples, which were analyzed pure (D) or with toothpaste (D + T); there were four experimental groups, corresponding to the method used to extract fluoride from the samples: “gastric juice” 30 min, “gastric juice” 60 min, “gastric juice” 120 min and microdiffusion facilitated by HMDS. The response variable was the F concentration, expressed in μg / mL. Obtaining diet samples (D) The sample size was statistically determined using the Bioestat software, based on data from previous studies, as follows: minimum difference between treatments = 0.15; stan- dard deviation = 0.1; study power = 0.8; level of significance = 0.05. The  result was 11, but we used 15 just in case. After authorization from the Municipal Education Secretary of Feira de Santana, Bahia, diet samples were collected at a daycare center. The meals were prepared with optimally fluoridated water (0.76 ± 0.01 μg F/mL) and consisted of rice, beans, pasta and meat. The cook placed the meals on dishes as usual, reproducing the amount usually consumed by children aged 2 to 3 years during lunch. Fifteen dishes were selected at random and each meal was collected separately in plastic containers. In the laboratory, samples of the diet were weighed, 100 mL of distilled and deionized water were added to each sample and homogenized with a mixer, without a filtration method. The final volume was determined, and each sample was frozen (- 18oC) until analysis. Knowing its volume before and after adding water, the dilution factor was calculated for each one. Obtaining diet + toothpaste samples (D+T) The used toothpaste was Tandy® (Colgate, strawberry flavor, lot 6289BR121K, validity 10/19), containing 942.8 ± 3.8 µg F/g as total soluble fluoride (TSF), from sodium fluoride. The TSF concentration was determined by the direct method using an ion-specific electrode18. According to a previous study7, children residing in Feira de Santana, Bahia, aged between 15 and 30  months, used an average of 0.47  g of toothpaste for brushing their teeth, and ingested 70.5% of this, resulting in 0.33 g of toothpaste ingested. In the present study, the average volume of the diet collected at the daycare center, sim- ulating lunch, was 250 mL. Hypothetically considering that children would brush their teeth right after lunch, the gastric content of these children would be 250 mL of diet and 0.33 g of toothpaste. We used this proportion to determine the amount of tooth- paste to be added to the diet + toothpaste samples (D+T), considering the volume of diet used in each analytic method: Ta = Da * Ti/ Di Where: • Ta: weight of toothpaste to be added to sample in laboratory analysis (g) • Da: volume of diet used in laboratory analysis (mL) • Ti: estimated weight of toothpaste ingested during toothbrushing (g) • Di: estimated volume of diet ingested per meal (mL) 4 Almeida et al. So, for microdiffusion facilitated by HMDS, Ta = 3 * 0.33/ 250 = 0.004 g. And for the “gastric juice”, Ta = 7 * 0.33/ 250 = 0.009 g. F determination using the “gastric juice” method (shown in Fig. 1) This simulation was performed by incubation of the samples in a solution that simulated gastric juice (0.01 M hydrochloric acid) at 37oC for 30, 60 and 120 min. This solution was called “gastric juice”. This protocol was based on the reported by Fernandes and Cury17 (1993), and in a preliminary study of our group19, which showed that the 7:1 proportion of sample to “gastric juice” resulted in a pH value corresponding to the gastric content during digestion (pH = 4.7612). In addition, the preliminary study showed that the incubation in “gas- tric juice” for 120 min resulted in a recovery of 95.51% of total F concentrations in samples. So, we decided to test this incubation time (120 min) against lower times (60 and 30 min). D samples were prepared adding 1 mL of 0.01 M HCl to 7 mL of diet. D+T samples were prepared adding 1 mL of 0.01 M HCl to 7 mL of diet and 0.009 g of toothpaste (based on the formulae previously demonstrated: Ta = Da * Ti/ Di). Plastic tubes con- taining the samples were incubated for 30, 60 or 120 min in an oven (Sterilifer, SX 300) at 37°C. Then centrifugation (Thermo Scientific) was performed for 5 min at 10,000 rpm. Fat was cautiously removed from the surface using an absorbent paper, and 0.4 mL of supernatant was buffered with 0.4 mL of TISAB II for F analysis. The F concentration was determined by means of an ion-specific electrode (ISE; Orion Model 96-09, Orion Research Incorporated, Cambridge, MA, USA) and an ion analyzer (Orion Star A214, Orion Research Incorporated), previously calibrated with standards solu- tions (0.4, 0.8, 1.6, 3.2 and 6.4  μg F/ mL) in triplicate, prepared in the same conditions of the samples. The calibration and concentrations determined were tested in the linear regression curve, using Excel software, where a calculation program transformed the val- ues of mV provided by the electrode in F concentration. Blank correction was done. D and D+T samples were analyzed in separate days. For D samples, the mean variation between obtained and expected values for calibration curve was 0.0085% and R2 = 0.9998. For D+T samples, the mean variation for calibration curve was 0.001% and R2 = 0.9999. read in F-specific electrode under stirring, and calculate [F] from mV readings using a spreadsheet D samples: 1 mL of 0.01 M HCl + 7 mL of diet or D + T samples: 0.009 g of toothpaste + 1 mL of 0.01 M HCl + 7 mL of diet mix vigorously ("vortex") 30, 60 or 120 min in an oven at 37o C centrifugate 5 min at 10,000 rpm remove fat from the surface with absorbent paper add 0.4 mL of supernatant + 0.4 mL of TISAB II Figure 1. F determination using the “gastric juice” method. 5 Almeida et al. F determination using microdiffusion facilitated by HMDS (shown in Fig. 2) It was based on the method described by Taves14 (1968). A cap from a plastic tube was placed, using vaseline, in the center of a plastic petri dish. For D samples, 3 mL of homogenized diet was added to the petri dish. For D+T samples, before the diet, 0.004 g of toothpaste was added to the petri dish (based on the formulae previously demonstrated: Ta = Da * Ti/ Di). For calibration curve, 1 mL of standard solution and 2 mL of distilled and deionized water were added to the petri dish. Then, 0.10 mL of 1.65 M sodium hydroxide was placed in the cap. The petri dish was closed, sealed with vaseline and 1.0 mL of 6 M hydrochloric acid saturated with HMDS added to the sample through a hole made in the petri dish cap. The hole was sealed with vaseline and the petri dish was shaken at room temperature for 14 h in an orbital shaker (Kline, NT-150). Then, the plastic cap containing F diffused from the sample was dried at 60°C for 2 h in an oven (Sterilifer, SX 300). This cap was then used to close a plastic tube containing 0.40 mL of 0.66 M acetic acid. The tube was inverted and vigorously shaken to dissolve the fluoride crystals present in the cap. The F concentration was determined by means of ISE, previously calibrated with standards solutions (0.4, 0.8, 1.6, 3.2 and 6.4 μg F/ mL) in triplicate, prepared in the same conditions of the samples. Blank correction was done. D and D+T samples were analyzed in separate days. For D samples, the mean variation between obtained and expected values for calibration curve was 0.054% and R2 = 0.9989. For D+T sam- ples, the mean variation for calibration curve was 1.423% and R2 = 0.9527. read in F-specific electrode and calculate [F] from mV readings using a spreadsheet fix a cap, using vaseline, in the center of a plastic petri dish add 3 mL of sample or 1 mL standard solution + 2 mL distilled and deionized water in the petri dish add 0.1 mL of 1.65 M NaOH in the central cap Close the petri dish, sealing with vaseline add 1 mL of 6 M HCl/ HMDS by the hole of petri´s dish, sealing imediatelly with vaseline leave 12-14h under agitation (orbital shaker) at room temperature remove the central cap and dry in an oven at 60o C for 2 h Use the cap to close a plastic tube containing 0.40 mL of 0.66 M acetic acid, invert and shake vigorously Figure 2. F determination using microdiffusion facilitated by HMDS. Statistical analysis A descriptive statistical analysis was performed to obtain values of central tendency and dispersion. Then, Analysis of Variance was used to compare the results of F concentration obtained for the different methods (one-way ANOVA + Tukey test). The coefficient of variation (CV%) and the intraclass correlation coefficient (ICC) were determined for all samples, analyzed in triplicate, to evaluate reproducibility. The soft- ware BioEstat 5.0 and SPSS were used; the level of significance was 5%. 6 Almeida et al. Results Results are expressed in table 1. Regarding diet (D) samples, “gastric juice” resulted in lower F concentrations than the microdiffusion facilitated by HMDS. Comparison among incubation times in “gastric juice” showed that 120  min resulted in higher F concentration than 30 and 60 min. Both the “gastric juice” method (CV% = 4.79% and ICC = 0.86) and the HMDS-facilitated microdiffusion (CV% = 6.58% and ICC = 0.89) showed high reproducibility20. The diet + toothpaste (D+T) samples comparison showed that the “gastric juice” with 60 and 120 min of incubation did not differ from HMDS-facilitated microdiffusion. The “gastric juice” method (CV% = 6.68% and ICC = 0.88) showed high reproducibility, but the HMDS-fa- cilitated microdiffusion (CV% = 10.45% and ICC = 0.42) showed low reproducibility. Table 1. Mean, standard deviation and range of F concentration (μg F/mL) in diet samples (D) and diet+toothpaste samples (D + T), using different methods of F extractiona. Method Diet (D) (n = 15) Diet+Toothpaste(D+T); (n = 15) microdiffusion facilitated by HMDS 1.02 ± 0.12 a (0.77 - 1.25) 2.99 ± 0.34 a (2.70 - 4.06) “gastric juice” 30 min 0.75 ± 0.06 c (0.63 - 0.88) 2.55 ± 0.46 b (1.25 – 3.16) 60 min 0.77 ± 0.07 c (0.69 - 0.92) 2.83 ± 0.44 ab (2.24 – 3.85) 120 min 0.91 ± 0.09 b (0.78 - 1.09) 3.15 ± 0.37 a (2.49 - 3.96) a Values followed by distinct letters indicate a statistically significant difference between the analytical techniques within each group (D or D+T) (P < 0.05; One-way ANOVA and Tukey’s test). Considering the results of the microdiffusion method as total F concentration, it was found that, using “gastric juice” for 30 min, 75.25% (± 10.77) of the total F were extracted from the samples of the diet. These values for 60 and 120 min were 77.33% (± 12.80) and 90.70% (± 9.69), respectively. For the samples of diet + toothpaste, using “gastric juice” for 30, 60 and 120 min extracted 86.90% (± 18.39), 94.83% (± 10.06) and 106.65% (± 15.96) of total F, respectively. Discussion After ingestion, the potentially bioavailable fluoride is represented by its soluble and in ionic form (F-), which can be converted into hydrofluoric acid (HF) and absorbed, having some systemic effect. The formation of HF depends on the pH of the medium. Gastric pH is acidic due to the presence of hydrochloric acid (HCl) in gastric juice. In  addition to HCl, gastric juice is composed of pepsinogen, intrinsic factor and mucus. The rate of HCl secretion varies depending on the stimuli. When the stomach is “empty”, it contains about 50  mL of gastric juice and its pH is approximately 221. After eating, the pH increases to 4.1 - 6.322. Therefore, it is necessary to assess the presence of potentially bioavailable fluoride in these conditions. In laboratory analysis, the electrode can detect F-. TISAB II is generally used as a buffer, in order to regulate the pH about 5.5, in which most of the soluble F will be 7 Almeida et al. in ionic form (F-)23. It must be considered that not all the F detected by the electrode represents the absorbed F, as there are different conditions for it to be detected in the laboratory (to be in the F- form)23 and to be absorbed in vivo (to be in the HF form)24. In an in vivo situation, perhaps the main reason for the difference between ingested and absorbed F is related to gastric pH. The F will only be absorbed in the form of hydrofluoric acid (HF)24, which is predominant when the pH of the gastrointestinal tract is less than 3.2 (pka value of HF). Therefore, depending on the gastric content, the pH might be different and therefore the absorption of F might also be different. Considering the gastric pH between 4.1 and 6.3, the predominant F will be in the ionic form (F-), therefore, it will not be absorbed24.The “gastric juice” method was proposed to simulate gastric conditions during digestion (pH and temperature). Thus, when the samples were subjected to these conditions, it was assumed that F would be solubi- lized in the same way that it occurs in real conditions. The results showed that there was no difference in F concentration obtained by using microdiffusion ou “gastric juice” for 120 min to extract F from samples of diet. It means that “gastric juice” method, with 120 min of incubation, can extract total F from diet samples. This is an important result, considering the advantages of the “gastric juice” method (simplicity and cost). The amount of F detected by the elec- trode analysis represents the ionic F, which might not be totally absorbed, since not all F- will form HF. Therefore, despite being potentially bioavailable, most of the F in the diet will not be absorbed. This could also justify the lack of correlation between F intake and dental fluorosis reported in the literature8-9. However, when the diet samples were incubated in “gastric juice” for shorter periods, less potentially bioavailable F was detected, showing that in the “gastric juice” method, the time of incubation affected the extraction of F from the diet samples. In fact, this method actually extracts fluoride from the samples, instead of just detecting the one already sol- uble; it is only a matter of time. Then additional studies are needed, to correlate shorter and longer incubation periods to in vivo conditions and assess the relationship between incubation time and potentially bioavailable F extraction from diet samples. Considering the samples of diet + toothpaste, it is possible that the duration of the incubation was important only for F in the diet, since the F concentrations were lower than the total F concentration only for the group incubated for 30 min. Perhaps the F in toothpaste, which contained sodium fluoride (NaF) and was already in ionic form, remained so, without being inactivated by cations present in food. It is also necessary to note that D + T analyzes using microdiffusion showed low reproducibility (ICC = 0.42). This was not expected, since microdiffusion is a well-es- tablished methodology for food15, and the toothpaste used contained sodium fluo- ride. Our hypothesis is that the toothpaste samples dried out because they were the first to be added (weighed). Only after toothpaste was added to all Petri dishes, the diet (homogenized food) was added. Although the Petri dish remained under agitation (orbital shaker) overnight, the toothpaste may not have adequately solubilized in food. To avoid this, it is suggested to add toothpaste after the diet. Among the limitations of the “gastric juice” method, is the fact that it is not as dynamic as the digestion process, where HCl secretions are not constant, so neither is the 8 Almeida et al. pH. In addition, gastric juice is not exclusively composed of HCl. Another limitation was that the samples were not under agitation while incubated at 37oC, therefore, peristaltic movements were not simulated, as suggested by Fernandes and Cury17 (1993). For the “gastric juice” method to be considered adequate to determine poten- tially bioavailable F, additional studies must be conducted, evaluating the effect of the following factors: absence of incubation time; repetition of analyzes over time (short periods) in the same sample until [F] reaches a plateau; addition and recovery of known amounts of F to samples. In addition, it must be considered that not all potentially bioavailable F will be absorbed, the “gastric juice” technique would also overestimate the dose of systemic exposure to fluoride and, consequently, the risk of dental fluorosis. Therefore, clinical studies, using validated biological samples that reflect fluoride metabolism are essential to complement in vitro studies16. Then, it can be concluded that the proposed method of “gastric juice” is a promising protocol for determining potentially bioavailable fluoride in the diet and toothpaste after ingestion. However, additional studies are desirable. Acknowledgements We are grateful to FAPESB (Bahia Research Foundation) for the master’s degree grant- ing (Process n. BOL 93/2016) and financial support (FAPESB/CNPq, n. 485/2011). References 1. Guha-Chowdhury N, Drummond BK, Smillie AC. Total fluoride intake in children aged 3 to 4 years - a longitudinal study. J Dent Res. 1996 Jul;75(7):1451-7. doi: 10.1177/00220345960750070401. 2. Rojas-Sanchez F, Kelly SA, Drake KM, Eckert GJ, Stookey GK, Dunipace AJ. Fluoride intake from foods, beverages and dentifrice by young children in communities with negligibly and optimally fluoridated water: a pilot study. Community Dent Oral Epidemiol. 1999 Aug;27(4):288-97. doi: 10.1111/j.1600-0528.1998.tb02023.x. 3. Lima YBO, Cury JA. [Fluoride intake by children from water and dentifrice]. Rev Saude Publica. 2001;35(6):576-81. doi: 10.1590/S0034-89102001000600012. Portuguese. 4. Paiva SM, Lima YBO, Cury JA. Fluoride intake by Brazilian children from two communities with fluoridated water. Comm Dent Oral Epidemiol. 2003 Jun;31(3):184-91. doi: 10.1034/j.1600- 0528.2003.00035.x. 5. Omena LMF, Silva MF, Pinheiro CC, Cavalcante JC, Sampaio FC. Fluoride intake from drinking water and dentifrice by children living in a tropical area of Brazil. J Appl Oral Sci. 2006 Oct;14(5):382-7. doi: 10.1590/s1678-77572006000500015. 6. Lima CV, Cury JA, Vale GC, Lima MD, Moura LF, Moura MS. Total fluoride intake by children frmo a tropical Brazilian city. Caries Res. 2015;49(6):640-6. doi: 10.1159/000442029. 7. Lima-Arsati YBO, Gomes ARLF, Santos HKA, Arsati F, Oliveira MC, Freitas VS. Exposure to fluoride of children during the critical age for dental fluorosis, in the semiarid region of Brazil. Cien Saude Colet. 2018 Apr;23(4):1045-1054. doi: 10.1590/1413-81232018234.07952016. Portuguese. 8. Martins CC, Paiva SM, Lima-Arsati YBO, Ramos-Jorge ML, Cury JA. Prospective study of the association between fluoride intake and dental fluorosis in permanent teeth. Caries Res. 2008;42(2):125-33. doi: 10.1159/000119520. https://doi.org/10.1590/S0034-89102001000600012 9 Almeida et al. 9. Warren JJ, Levy SM, Broffitt B, Cavanaugh JE, Kanellis MJ, Weber-Gasparoni K. Considerations on optimal fluoride intake using dental fluorosis and dental caries outcomes - a longitudinal study. J Public Health Dent. 2009;69(2):111-5. doi: 10.1111/j.1752-7325.2008.00108.x. 10. Cury JA, Del Fiol FS, Tenuta LMA. Low-fluoride dentifrice and gastrointestinal fluoride absorption after meals. J Dent Res. 2005 Dec;84(12):1133-7. doi: 10.1177/154405910508401208. 11. Falcão A, Tenuta LMA, Cury JA. Fluoride gastrointestinal absorption from Na2FPO3 /CaCO3 - and NaF/SiO2 – based toothpastes. Caries Res. 2013;47(3):226-33. doi: 10.1159/000346006. 12. Martinez-Mier EA, Tenuta LMA, Carey CM, Cury JA, Van Loveren C, Ekstrand KR, et al. ORCA Fluoride in Toothpaste Analysis Work Group. European Organization for Caries Research Workshop: Methodology for Determination of Potentially Available Fluoride in Toothpastes. Caries Res. 2019;53(2):119-36. doi: 10.1159/000490196. 13. Reshetnyak VY, Nesterova OV, Admakin OI, Dobrokhotov DA, Avertseva IN, Dostdar SA, et al. Evaluation of free and total fluoride concentration in mouthwashes via measurement with ion- selective electrode. BMC Oral Health. 2019 Nov 20;19(1):251. doi: 10.1186/s12903-019-0908-0. 14. Taves DR. Separation of fluoride by rapid diffusion using hexamethyldisiloxane. Talanta. 1968 Sep;15(9):969-74. doi: 10.1016/0039-9140(68)80097-9. 15. Martínez-Mier EA, Cury JA, Heilman JR, Katz BP, Levy SM, Li Y, et al. Development of gold standard ion-selective electrode-based methods for fluoride analysis. Caries Res. 2011;45(1):3-12. doi: 10.1159/000321657. 16. Idowu OS, Azevedo LB, Valentine RA, Swan J, Vasantavada PV, Maguire A, et al. The use of urinary fluoride excretion to facilitate monitoring fluoride intake: a systematic scoping review. PLoS One. 2019 Sep;14(9):e0222260. doi: 10.1371/journal.pone.0222260. 17. Fernandes LMAG, Cury JA. [Prenatal fluoride - metabolic evaluation]. RBM Rev Bras Med. 1993 Nov;50(11):1546-52. Portuguese. 18. Cury JA, Oliveira MJL, Martins CC, Tenuta LM, Paiva SM. Available fluoride in toothpastes used by Brazilian children. Braz. Dent. J. 2010;21(5):396-400. doi: 10.1590/s0103-64402010000500003. 19. Lima-Arsati YBO, Santos HKA, Gomes ARLF. Evaluation of a protocol to simulate in vitro the decreased absorption of fluoride from toothpaste due to gastric content. In: XIX ABOPREV Meeting. Braz J Oral Sci. 2015;15(1):87. doi: 10.20396/bjos.v14i1.8641314. 20. Munro BH. Statistical methods for health care research. 3rd ed. New York: Lippincott Williams & Wilkins; 1997. 21. Hall JE. Guyton and Hall textbook of medical physiology. 13. ed. Philadelphia, PA: Elsevier; 2016. 22. McLauchlan G, Fullarton GM, Crean GP, McColl KE. Comparison of gastric body and antral pH: a 24 hour ambulatory study in healthy volunteers. Gut. 1989 May;30(5):573-8. doi: 10.1136/ gut.30.5.573. 23. Thermo Fisher Scientific. Thermo Scientific Orion Fluoride Ion Selective Electrode User Guide. 254792-001, Revision B. 2016 Sep [cited 2020 Jul 23]. Available from: https://www.thermofisher.com/document-connect/document-connect. html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FLSG%2Fmanuals% 2FD15872~.pdf&title=Rmx1b3JpZGUgSW9uIFNlbGVjdGl2ZSBFbGVjdHJvZGUgLSBVc2VyIEd1aWRl. 24. Whitford GM. Absorption and plasma concentrations of fluoride. In: Whitford GM. The metabolism and toxicity of fluoride. 2. ed. Basel: Karger; 1996. Chapter 2, p.10-29. doi: 10.1159/ isbn.978-3-318-04022-7.